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Bone marrow aspirate concentrate

Graeme P Whyte, Alberto Gobbi, Boguslaw Sadlik
Cartilage lesions of the knee are a frequent finding; however, treatment options that are capable of restoring hyaline-like tissue are not routinely used. Cell-based technology such as autologous chondrocyte implantation may in some cases provide durable cartilage repair, but availability of this procedure is often restricted due to cost constraints. There have been promising outcomes reported with the use of scaffolds seeded with activated bone marrow aspirate concentrate in cases of chondral injury. There are clear advantages to cell-based cartilage repair techniques that are performed as a single-stage procedure, particularly when the repair technology can be used in a minimally invasive manner...
August 2016: Arthroscopy Techniques
Muthu Gopal, Sathishkumar Elumalai, Suresh Arumugam, Vishnuprabu Durairajpandian, Munirajan Arasambattu Kannan, Esthermary Selvam, Srivani Seetharaman
INTRODUCTION: Typhoid fever is endemic in India and other developing countries, causing major public health problems with high morbidity and mortality. The resistance of Salmonella enterica serovar Typhi (S. Typhi) towards commonly prescribed antimicrobials is increasing in developing countries. However, there have been several reports of the therapeutic failure of fluoroquinolones in patients with Salmonella infection. Resistance to quinolones/ fluoroquinolones commonly arises due to target site mutation...
July 2016: Journal of Clinical and Diagnostic Research: JCDR
Chester Joyner, Alberto Moreno, Esmeralda V S Meyer, Monica Cabrera-Mora, Jessica C Kissinger, John W Barnwell, Mary R Galinski
BACKGROUND: Plasmodium vivax infections in humans or in new world monkeys pose research challenges that necessitate the use of alternative model systems. Plasmodium cynomolgi is a closely related species that shares genetic and biological characteristics with P. vivax, including relapses. Here, the haematological dynamics and clinical presentation of sporozoite-initiated P. cynomolgi infections in Macaca mulatta (rhesus macaques) are evaluated over a 100-day period. METHODS: Five M...
2016: Malaria Journal
Janina Frisch, Patrick Orth, Jagadeesh Kumar Venkatesan, Ana Rey-Rico, Gertrud Schmitt, Dieter Kohn, Henning Madry, Magali Cucchiarini
: : Transplantation of genetically modified peripheral blood aspirates that carry chondrogenically competent progenitor cells may offer new, convenient tools to treat articular cartilage lesions compared with the more complex and invasive application of bone marrow concentrates or of bone marrow-derived mesenchymal stem cells. Here, we show that recombinant adeno-associated viral (rAAV) vectors are powerful gene vehicles capable of successfully targeting primary human peripheral blood aspirates in a stable and safe manner, allowing for an efficient and long-term transgene expression in such samples (up to 63 days with use of a lacZ reporter gene and for at least 21 days with application of the pleiotropic, chondrogenic factor transforming growth factor-β [TGF-β])...
August 29, 2016: Stem Cells Translational Medicine
Shane A Shapiro, Shari E Kazmerchak, Michael G Heckman, Abba C Zubair, Mary I O'Connor
BACKGROUND: Bone marrow aspirate concentrate (BMAC) is increasingly used as a regenerative therapy for musculoskeletal pathological conditions despite limited evidence-based support. HYPOTHESIS: BMAC will prove feasible, safe, and efficacious for the treatment of pain due to mild to moderate degenerative joint disease of the knee. STUDY DESIGN: Randomized controlled trial; Level of evidence, 2. METHODS: In this prospective, single-blind, placebo-controlled trial, 25 patients with bilateral knee pain from bilateral osteoarthritis were randomized to receive BMAC into one knee and saline placebo into the other...
August 26, 2016: American Journal of Sports Medicine
Steven Sampson, Jay Smith, Hunter Vincent, Danielle Aufiero, Mona Zall, Angie Botto-van-Bemden
AIM: Evaluate intra-articular injection of bone marrow concentrate (BMC), followed by platelet-rich plasma (PRP) injection at 8 weeks follow-up in moderate/severe osteoarthritis. DESIGN: Single center, retrospective Case Series (n = 125). METHODS: Bone marrow was aspirated/concentrated using a standardized technique. Patients received a single intra-articular injection of BMC, with follow-up injection of PRP at 8 weeks. RESULTS: Median absolute pain reduction in all joints was five points (71...
September 2016: Regenerative Medicine
Emily T Camilleri, Michael P Gustafson, Amel Dudakovic, Scott M Riester, Catalina Galeano Garces, Christopher R Paradise, Hideki Takai, Marcel Karperien, Simon Cool, Hee-Jeong Im Sampen, A Noelle Larson, Wenchun Qu, Jay Smith, Allan B Dietz, Andre J van Wijnen
BACKGROUND: Clinical translation of mesenchymal stromal cells (MSCs) necessitates basic characterization of the cell product since variability in biological source and processing of MSCs may impact therapeutic outcomes. Although expression of classical cell surface markers (e.g., CD90, CD73, CD105, and CD44) is used to define MSCs, identification of functionally relevant cell surface markers would provide more robust release criteria and options for quality control. In addition, cell surface expression may distinguish between MSCs from different sources, including bone marrow-derived MSCs and clinical-grade adipose-derived MSCs (AMSCs) grown in human platelet lysate (hPL)...
2016: Stem Cell Research & Therapy
Sung Woo Huh, Asode Ananthram Shetty, Saif Ahmed, Dong Hwan Lee, Seok Jung Kim
Degenerative and traumatic articular cartilage defects are common, difficult to treat, and progressive lesions that cause significant morbidity in the general population. There have been multiple approaches to treat such lesions, including arthroscopic debridement, microfracture, multiple drilling, osteochondral transplantation and autologous chondrocyte implantation (ACI) that are currently being used in clinical practice. Autologous bone-marrow mesenchymal cell induced chondrogenesis (MCIC) is a single-staged arthroscopic procedure...
July 2016: Journal of Clinical Orthopaedics and Trauma
Alberto Gobbi, Graeme P Whyte
BACKGROUND: Articular cartilage injury is frequently encountered, yet treatment options capable of providing durable cartilage repair are limited. PURPOSE: To investigate the medium-term clinical outcomes of cartilage repair using a 1-stage technique of a hyaluronic acid-based scaffold with activated bone marrow aspirate concentrate (HA-BMAC) and compare results with those of microfracture. A secondary aim of this study was to identify specific patient demographic factors and cartilage lesion characteristics that are associated with superior outcomes...
July 29, 2016: American Journal of Sports Medicine
Janina Frisch, Jagadeesh K Venkatesan, Ana Rey-Rico, Adam M Zawada, Gertrud Schmitt, Henning Madry, Magali Cucchiarini
BACKGROUND: Application of genetically modified bone marrow concentrates in articular cartilage lesions is a promising approach to enhance cartilage repair by stimulating the chondrogenic differentiation processes in sites of injury. METHOD: In the present study, we examined the potential benefits of transferring the proliferative and pro-chondrogenic basic fibroblast growth factor (FGF-2) to human bone marrow aspirates in vitro using the clinically adapted recombinant adeno-associated virus (rAAV) vectors to monitor the biological and chondrogenic responses over time to the treatment compared with control (lacZ) gene application...
December 2016: Journal of Experimental Orthopaedics
Kenneth K Hansraj
INTRODUCTION: Spine surgeons are embracing advanced biologic technologies in an attempt to help millions of people achieve a better outcome in spine surgery. These new technologies may be complicated to understand, partly because the contribution of different types of cells has not been definitively identified. This paper describes the characteristics of the stem cells used in spine surgery, including their actions and possible complications. The description necessitates an overview of all studies to date on the use of stem cells in spine surgery, as well as other cells used in cellular therapy...
July 29, 2016: Surgical Technology International
Todd P Pierce, Casey Wagner, Vincent K McInerney, Anthony Festa, Anthony J Scillia
Tears of the meniscal capsular junction present unique diagnostic and treatment challenges for practitioners because they are rich in blood supply yet often not visualized on magnetic resonance imaging. The authors report the case of a young man with a meniscal capsular junction tear, difficulties related to his diagnosis, and his short-term outcomes following surgical treatment. An 18-year-old male cross-country runner presented to the authors for nonradiating lateral right knee pain after a failed course of physical therapy...
July 19, 2016: Orthopedics
Jeanette Johnson, Jonathon Jundt, Issa Hanna, Jonathan W Shum, Gary Badger, James C Melville
BACKGROUND AND OVERVIEW: Ameloblastoma is an odontogenic tumor predominantly occurring in patients who are in their 20s and 30s. Approximately 10% to 15% of ameloblastomas occur in patients younger than 18 years. Although it is a benign tumor, an ameloblastoma can have a devastating effect on children both physically and emotionally. The aim of this case report is to demonstrate how tissue engineering and surgical techniques can minimize morbidity and recovery time after extirpation and immediate reconstruction of a mandibular ameloblastoma...
July 16, 2016: Journal of the American Dental Association
M Alonso, L Builes, P Morán, A Ortega, E Fernández, F Reinoso-Barbero
BACKGROUND: Desflurane has been used in paediatric patients for several surgical indications. This article analyses the efficacy and safety of desflurane for diagnostic-therapeutic procedures in remote areas far from operating room in a group of selected patients with no known associated respiratory disease. MATERIAL AND METHODS: A retrospective analysis was performed on 2,072 general anaesthesia procedures stored in a computer database, in which desflurane was used in a Paediatric Pain Unit during the years 2013 and 2014...
July 2, 2016: Revista Española de Anestesiología y Reanimación
Gherardo Pagliazzi, Matteo Baldassarri, Luca Perazzo, Francesca Vannini, Francesco Castagnini, Roberto Buda
BACKGROUND: Juvenile osteochondritis dissecans of the talus (JOCDT) is a focal idiopathic lesion primarily of the subchondral bone leading to subsequent cartilaginous damage. The majority of the papers dealing with JOCDT reported heterogeneous case studies of patients treated with different cartilage repair techniques. The purpose of this paper is to retrospectively review both clinical and radiologic results among 7 patients affected by JOCDT treated with arthroscopic bone marrow aspirate concentrate (BMAC) transplantation with the 1-step technique...
July 2, 2016: Journal of Pediatric Orthopedics
James Holton, Mohamed A Imam, Martin Snow
The ability of mesenchymal stem cells (MSCs) to transdifferentiate into a desired cell lineage has captured the imagination of scientists and clinicians alike. The limited ability for chondrocytes to regenerate in chondral injuries has raised the concept of using MSCs to help regenerate and repair damaged tissue. The expansion of cells in a laboratory setting to be delivered back to the patient is too costly for clinical use in the present tough economic climate. This process is slow with due to the complexity of trying to imitate the natural environment and biological stimulation of chondral cell replication and proliferation...
2016: Frontiers in Surgery
Jorge Chahla, Robert F LaPrade, Rodrigo Mardones, Johnny Huard, Marc J Philippon, Shane Nho, Omer Mei-Dan, Cecilia Pascual-Garrido
Treatment of hip cartilage disease is challenging, and there is no clear algorithm to address this entity. Biomarkers are arising as promising diagnostic tools because they could play a role in the early assessment of the prearthritic joint and as a prognostic factor before and after treatment. The potential effect of biomarkers may be used to categorize individuals at risk of evolving to severe osteoarthritis, to develop new measures for clinical progression of the disease, and to develop new treatment options for the prevention of osteoarthritis progression...
July 1, 2016: Orthopedics
Amanda M Meppelink, Xing-Hua Wang, Gino Bradica, Kathryn Barron, Kathleen Hiltz, Xiang-Hong Liu, Scott M Goldman, Joseph P Vacanti, Armand Keating, David M Hoganson
BACKGROUND AIMS: The use of bone marrow-derived mesenchymal stromal cells (MSCs) in cell-based therapies is currently being developed for a number of diseases. Thus far, the clinical results have been inconclusive and variable, in part because of the variety of cell isolation procedures and culture conditions used in each study. A new isolation technique that streamlines the method of concentration and demands less time and attention could provide clinical and economic advantages compared with current methodologies...
June 2016: Cytotherapy
Iva Hoffmanová, Petra Kačírková, Irena Kučerová, Rudolf Ševčík, Daniel Sánchez
This article reports on patient that has been presented with sudden onset of constipation, abdominal pain and normocytic anemia. Gastroscopy and colonoscopy ruled out an organic diseases. In peripheral blood and bone marrow aspirates mears, coarse basophilic stippling of erythrocyte (and erythroblasts) point out a possibility of heavy metal poisoning. The level of plumbemia exceeded 8.4 times the maximal permitted value for common (non-professional) population. A source of poisoning was indentified from a glaze on a ceramic jug, from which the patient had drank tea with lemon for three months...
February 2016: Vnitr̆ní Lékar̆ství
Giovanna Desando, Gianluca Giavaresi, Carola Cavallo, Isabella Bartolotti, Federica Sartoni, Nicolò Nicoli Aldini, Lucia Martini, Annapaola Parrilli, Erminia Mariani, Milena Fini, Brunella Grigolo
INTRODUCTION: Cell-based therapies are becoming a valuable tool to treat osteoarthritis (OA). This study investigated and compared the regenerative potential of bone marrow concentrate (BMC) and mesenchymal stem cells (MSC), both engineered with Hyaff(®)-11 (HA) for OA treatment in a sheep model. METHODS: OA was induced via unilateral medial meniscectomy. Bone marrow was aspirated from the iliac crest, followed by concentration processes or cell isolation and expansion to obtain BMC and MSC, respectively...
June 2016: Tissue Engineering. Part C, Methods
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