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Spermatogonia

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https://www.readbyqxmd.com/read/29222434/embryonic-lethality-and-defective-male-germ-cell-development-in-mice-lacking-utf1
#1
Seth D Kasowitz, Mengcheng Luo, Jun Ma, N Adrian Leu, P Jeremy Wang
The germ cell lineage is specified early in embryogenesis and undergoes complex developmental programs to generate gametes. Here, we conducted genetic studies to investigate the role of Utf1 (Undifferentiated embryonic cell transcription factor 1) in mouse germ cell development. Utf1 is expressed in pluripotent embryonic stem (ES) cells and regulates ES cell differentiation. In a proteomics screen, we identified UTF1 among 38 proteins including DNMT3L and DND1 that associate with chromatin in embryonic testes...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29209278/follicle-stimulating-hormone-regulates-igfbp-gene-expression-directly-or-via-downstream-effectors-to-modulate-igf3-effects-on-zebrafish-spermatogenesis
#2
Diego Safian, Henk J G van der Kant, Diego Crespo, Jan Bogerd, Rüdiger W Schulz
Previous work showed that pharmacological inactivation of Igf-binding proteins (Igfbps), modulators of Igf activity, resulted in an excessive differentiation of type A undifferentiated (Aund) spermatogonia in zebrafish testis in tissue culture when Fsh was present in the incubation medium. Using this testis tissue culture system, we studied here the regulation of igfbp transcript levels by Fsh and two of its downstream effectors, Igf3 and 11-ketotestosterone (11-KT). We also explored how Fsh-modulated igfbp expression affected spermatogonial proliferation by adding or removing the Igfbp inhibitor NBI-31772 at different times...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29192358/cryopreservation-of-male-and-female-gonial-cells-by-vitrification-in-the-critically-endangered-cyprinid-honmoroko-gnathopogon-caerulescens
#3
Shogo Higaki, Takaaki Todo, Reiko Teshima, Ikuo Tooyama, Yasuhiro Fujioka, Noriyoshi Sakai, Tatsuyuki Takada
We investigated the feasibility of cryopreservation of spermatogonia and oogonia in the critically endangered cyprinid honmoroko Gnathopogon caerulescens using slow-cooling (freezing) and rapid-cooling (vitrification) methods. Initially, we examined the testicular cell toxicities and glass-forming properties of the five cryoprotectants: ethylene glycol (EG), glycerol (GC), dimethyl sulfoxide (DMSO), propylene glycol (PG), and 1,3-butylene glycol (BG), and we determined cryoprotectant concentrations that are suitable for freezing and vitrification solutions, respectively...
November 30, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29186436/transcriptome-profiling-of-fetal-klinefelter-testis-tissue-reveals-a-possible-involvement-of-long-non-coding-rnas-in-gonocyte-maturation
#4
Sofia B Winge, Marlene D Dalgaard, Jacob M Jensen, Niels Graem, Mikkel H Schierup, Anders Juul, Ewa Rajpert-De Meyts, Kristian Almstrup
In humans, the most common sex chromosomal disorder is Klinefelter syndrome (KS), caused by the presence of one or more extra X-chromosomes. KS patients display a varying adult phenotype but usually present with azoospermia due to testicular degeneration, which accelerates at puberty. The timing of the germ cell loss and whether it is caused by dysgenetic fetal development of the testes is not known.We investigated 8 fetal KS testes and found a marked reduction in MAGE-A4-positive pre-spermatogonia compared to testes from 15 age-matched controls, indicating a failure of the gonocytes to differentiate into pre-spermatogonia...
November 24, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29183964/regulation-of-blood-testis-barrier-assembly-in-vivo-by-germ-cells
#5
Xiao-Yu Li, Yan Zhang, Xiu-Xia Wang, Cheng Jin, Yu-Qian Wang, Tie-Cheng Sun, Jian Li, Ji-Xin Tang, Alia Batool, Shou-Long Deng, Su-Ren Chen, C Yan Cheng, Yi-Xun Liu
The assembly of the blood-testis barrier (BTB) during postnatal development is crucial to support meiosis. However, the role of germ cells in BTB assembly remains unclear. Herein, KitW/KitWV mice were used as a study model. These mice were infertile, failing to establish a functional BTB to support meiosis due to c-Kit mutation. Transplantation of undifferentiated spermatogonia derived from normal mice into the testis of KitW/KitWV mice triggered functional BTB assembly, displaying cyclic remodeling during the epithelial cycle...
November 28, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29179435/genome-wide-5-hydroxymethylcytosine-patterns-in-human-spermatogenesis-are-associated-with-semen-quality
#6
Olga A Efimova, Anna A Pendina, Andrei V Tikhonov, Sergey E Parfenyev, Irina D Mekina, Evgeniia M Komarova, Mariia A Mazilina, Eugene V Daev, Olga G Chiryaeva, Ilona A Galembo, Mikhail I Krapivin, Oleg S Glotov, Irina S Stepanova, Svetlana A Shlykova, Igor Yu Kogan, Alexander M Gzgzyan, Tatyana V Kuznetzova, Vladislav S Baranov
We performed immunofluorescent analysis of DNA hydroxymethylation and methylation in human testicular spermatogenic cells from azoospermic patients and ejaculated spermatozoa from sperm donors and patients from infertile couples. In contrast to methylation which was present throughout spermatogenesis, hydroxymethylation was either high or almost undetectable in both spermatogenic cells and ejaculated spermatozoa. On testicular cytogenetic preparations, 5-hydroxymethylcytosine was undetectable in mitotic and meiotic chromosomes, and was present exclusively in interphase spermatogonia Ad and in a minor spermatid population...
October 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/29179259/roles-of-histone-h3-5-in-human-spermatogenesis-and-spermatogenic-disorders
#7
K Shiraishi, A Shindo, A Harada, H Kurumizaka, H Kimura, Y Ohkawa, H Matsuyama
Histone H3.5 (H3.5) is a newly identified histone variant highly expressed in the human testis. We have reported the crystal structure, instability of the H3.5 nucleosome and accumulation around transcription start sites, mainly in primary spermatocytes, but its role in human spermatogenesis remains poorly understood. Testicular biopsy specimens from 30 men (mean age: 35 years) with non-obstructive azoospermia (NOA) who underwent microdissection testicular sperm extraction and 23 men with obstructive azoospermia (OA) were included...
November 27, 2017: Andrology
https://www.readbyqxmd.com/read/29179007/electron-microscopy-observations-on-testis-and-spermatozoa-of-leptodactylus-chaquensis-anura-leptodactylidae
#8
Lucrecia Iruzubieta Villagra, Inés Ramos, Susana Cisint, Claudia A Crespo, Silvia N Fernández
Scanning and transmission electron microscopy were used to investigate the fine structure of the testis and spermatozoa of toad Leptodactylus chaquensis. Our observations indicate that germinal compartment contains Sertoli (SC) and germ cells. The tight junctions and desmosomes among SC indicate the existence of an hematotesticular barrier in charge of maintaining the differences in the composition of the germinal and interstitial compartments. During spermatogenesis, SC acts as a structural support for germ cells...
November 21, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29176590/nanocarrier-mediated-delivery-of-%C3%AE-mangostin-for-non-surgical-castration-of-male-animals
#9
Jakarwan Yostawonkul, Suvimol Surassmo, Katawut Namdee, Mattaka Khongkow, Chatwalee Boonthum, Sasithon Pagseesing, Nattika Saengkrit, Uracha Rungsardthong Ruktanonchai, Kaywalee Chatdarong, Suppawiwat Ponglowhapan, Teerapong Yata
The overpopulation of abandoned and stray companion animals has become a global crisis. The main purpose of this study was to develop a novel nanomedicine-based antifertility compound for non-surgical castration of male animals. Mangosteen (Garcinia mangostana L) pericarp extract has been shown to exhibit anti-fertility property. α-mangostin (AM)-loaded nanostructured lipid carrier (AM-NLC) was developed to improve male germ cell apoptosis. This study was conducted to investigate physicochemical properties of AM-NLC and determine the biological effects of AM-NLC on spermatogonia cells and testicular explants obtained from castrated testes...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29174386/expression-and-cellular-localization-of-double-sex-and-mab-3-related-transcription-factor-1-in-testes-of-postnatal-small-tail-han-sheep-at-different-developmental-stages
#10
Taotao Li, Zengkui Lu, Ruirui Luo, Jianfeng Gao, Xingxu Zhao, Youji Ma
Double sex and mab-3 related transcription factor 1 (Dmrt1), an evolutionarily conserved gene, is a sex-related gene expressed in male gonads, that is involved in the regulation of sex differentiation, testicular development and reproductive function maintenance. Until now, functional studies on the Dmrt1 gene in sheep (Ovis aries) have been lacking. In this study, testis, heart, liver, spleen, lung, kidney and longissimus dorsi muscle tissues were collected from Small-Tail Han sheep at 0, 2, 5, 12 and 24months after birth (mab)...
November 22, 2017: Gene
https://www.readbyqxmd.com/read/29169284/vegfc-vegfr3-signaling-regulates-mouse-spermatogonial-cell-proliferation-via-the-activation-of-akt-mapk-and-cyclin-d1-pathway-and-mediates-the-apoptosis-by-affecting-caspase-3-9-and-bcl-2
#11
Liangyu Zhao, Zijue Zhu, Chencheng Yao, Yuhua Huang, Erlei Zhi, Huixing Chen, Ruhui Tian, Peng Li, Qingqing Yuan, Yunjing Xue, Zhong Wan, Chao Yang, Yuehua Gong, Zuping He, Zheng Li
We have previously shown that the transcript levels of Vegfc and its receptor Vegfr3 were high in spermatogonia and extremely low in spermatocytes and spermatids. However, it remains unknown about the functions and the mechanisms of VEGFC/VEGFR3 signaling in regulating the fate determinations of spermatogonia. To this end, here we explored the role and signaling pathways of VEGFC/VEGFR3 by using a cell line derived from immortalized mouse spermatogonia retaining markers of mitotic germ cells, namely GC-1 cells...
November 23, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29161703/expression-profile-of-notch3-in-mouse-spermatogonia
#12
Ryu Okada, Megumi Fujimagari, Eri Koya, Yoshikazu Hirose, Tomomi Sato, Yukio Nishina
Stable and sustainable spermatogenesis is supported by the strict regulation of self-renewal and differentiation of spermatogonial stem cells (SSC), which are a rare population of undifferentiated spermatogonia. It has been revealed that some signaling factors regulate the self-renewal of SSC; however, the molecular mechanism of SSC maintenance is still not completely understood. Notch signaling is an evolutionarily conserved juxtacrine signaling that plays important roles in the cell fate determination of various tissue stem cells...
January 22, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29161153/protective-effects-of-agomelatine-on-testicular-damage-caused-by-bortezomib
#13
N Akaras, T Bal, H Atilay, J Selli, M B Halici
Bortezomib is a chemotherapeutic agent used to treat several cancers; however, it exhibits severe side effects in testicular tissue. We investigated the use of agomelatine to prevent testicular tissue damage caused by bortezomib. We used 36 male Sprague-Dawley rats divided randomly into six equal groups: group 1, no treatment control; group 2, agomelatine treatment only; group 3, bortezomib treatment only for 48 h; group 4, bortezomib + agomelatine treatment for 48 h; group 5, bortezomib treatment only for 72 h; and group 6, bortezomib + agomelatine treatment for 72 h...
November 21, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29156418/characterization-of-domestic-pig-spermatogenesis-using-spermatogonial-stem-cell-markers-in-the-early-months-of-life
#14
Julio Almunia, Kotono Nakamura, Mami Murakami, Shigeo Takashima, Masaki Takasu
In pigs, spermatogonial stem cells (SSCs), which include gonocytes and undifferentiated spermatogonia, are classically defined as being present till up to 2 months of life. However, knowledge about this transition from gonocytes to undifferentiated spermatogonia in pigs is limited. In this study, we characterized pig SSCs in neonatal (n = 6), one-month-old (1-mo-old, n = 6), two-month-old (2-mo-old, n = 6), and adult testes (n = 6) anatomically, histologically, and immunohistologically. Anatomically, testicular circumference (TC) was measured at each development stage...
November 3, 2017: Theriogenology
https://www.readbyqxmd.com/read/29155330/expression-pattern-and-functional-analysis-of-r-spondin1-in-tongue-sole-cynoglossus-semilaevis
#15
Jinxiang Liu, Tiantian Liu, Jingjing Niu, Xiaolong Wu, Jieming Zhai, Quanqi Zhang, Jie Qi
R-spondin 1 (Rspo1) is a potential female-determining gene in mammals that could regulate the Wnt/β-catenin signaling pathway. The deletion of Rspo1 causes sex reversal in females. To investigate sexual determination and differentiation, we cloned and analyzed the Rspo1 gene in Cynoglossus semilaevis. Phylogenetic and gene structure analyses revealed that Rspo1 gene exhibited high sequence conservation and contained an N-terminal signal peptide, two furin-like cysteine-rich domains (FU1 and FU2), a thrombospondin type 1 repeat, and a C-terminal region enriched with basic charged amino acids...
November 16, 2017: Gene
https://www.readbyqxmd.com/read/29150651/comprehensive-functional-enrichment-analysis-of-male-infertility
#16
Seyed Morteza Razavi, Marjan Sabbaghian, Mahdi Jalili, Adeleh Divsalar, Olaf Wolkenhauer, Ali Salehzadeh-Yazdi
Spermatogenesis is a multifactorial process that forms differentiated sperm cells in a complex microenvironment. This process involves the genome, epigenome, transcriptome, and proteome to ensure the stability of the spermatogonia and supporting cells. The identification of signaling pathways linked to infertility has been hampered by the inherent complexity and multifactorial aspects of spermatogenesis. Systems biology is a promising approach to unveil underlying signaling pathways and genes and identify putative biomarkers...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29126714/testicular-steroidogenic-enzymes-in-the-lizard-podarcis-sicula-during-the-spermatogenic-cycle
#17
Luigi Rosati, Alessandra Santillo, Maria Maddalena Di Fiore, Piero Andreuccetti, Marina Prisco
Steroidogenic Acute Regulatory protein (StAR), 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD), 5α-Reductase (5α-Red), P450 aromatase are key enzymes involved in steroidogenesis. Recently, we showed the expression and the localization of P450 aromatase in Podarcis sicula testis during the different phases of the reproductive cycle, showing its involvement in the control of steroidogenesis, particularly in 17β-estradiol synthesis. Now, we have investigated the presence and distribution of the other enzymes involved in steroidogenesis, i...
November 7, 2017: Comptes Rendus Biologies
https://www.readbyqxmd.com/read/29119857/mir-638-inhibits-immature-sertoli-cell-growth-by-indirectly-inactivating-pi3k-akt-pathway-via-spag1-gene
#18
Pandi Hu, Kaifeng Guan, Yue Feng, Changping Ma, Huibin Song, Yang Li, Xuanyan Xia, Jialian Li, Fenge Li
Numerous studies have demonstrated that microRNAs (miRNAs) play important roles in cell growth, apoptosis and spermatogenesis. Our previous study showed that miR-638 was differentially expressed in sexually immature and mature testes of Large White boars. Here we reported that sperm-associated antigen 1 (SPAG1) was a direct target gene of miR-638. Moreover, miR-638 inhibited cell proliferation and cell cycle, and promoted apoptosis of porcine immature Sertoli cells. Key genes including phosphorylated phosphatidylinositide 3-kinases (p-PI3K) and phosphorylated serine/ threonine kinase (p-AKT) in PI3K/AKT pathway as well as cell cycle factors including c-MYC, cyclin-D1 (CCND1), cyclin-E1 (CCNE1) and cyclin-dependent kinase 4 (CDK4) were all significantly down-regulated after overexpression of miR-638 or RNAi of SPAG1...
November 9, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29113556/phospholipase-d-family-member-6-pld6-is-a-surface-marker-for-enrichment-of-undifferentiated-spermatogonia-in-the-pre-pubertal-boars
#19
Pengfei Zhang, Yuwei Qin, Yi Zheng, Wenxian Zeng
Spermatogonial stem cells (SSCs) have a functional ability to maintain self-renewal and sustain production of spermatozoa throughout the reproductive lifespan of a male. Studies on SSCs can thus better understandings of spermatogenesis and unravel the mechanisms for self-renewal and differentiation of male germline stem cells. However, the rarity of SSCs in the testis and the lack of reliable surface markers obstruct the related study and further application of SSCs. This is especially the case in livestock animals...
November 7, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29109271/periodic-production-of-retinoic-acid-by-meiotic-and-somatic-cells-coordinates-four-transitions-in-mouse-spermatogenesis
#20
Tsutomu Endo, Elizaveta Freinkman, Dirk G de Rooij, David C Page
Mammalian spermatogenesis is an elaborately organized differentiation process, starting with diploid spermatogonia, which include germ-line stem cells, and ending with haploid spermatozoa. The process involves four pivotal transitions occurring in physical proximity: spermatogonial differentiation, meiotic initiation, initiation of spermatid elongation, and release of spermatozoa. We report how the four transitions are coordinated in mice. Two premeiotic transitions, spermatogonial differentiation and meiotic initiation, were known to be coregulated by an extrinsic signal, retinoic acid (RA)...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
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