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Spermatogonia

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https://www.readbyqxmd.com/read/28087628/id4-levels-dictate-the-stem-cell-state-in-mouse-spermatogonia
#1
Aileen R Helsel, Qi-En Yang, Melissa J Oatley, Tessa Lord, Fred Sablitzky, Jon M Oatley
Spermatogenesis is a classic model of cycling cell lineages that depend on a balance between stem cell self-renewal for continuity and formation of progenitors as the initial step in production of differentiated cells. However, the mechanisms guiding the continuum of spermatogonial stem cell (SSC) to progenitor spermatogonial transition and precise identifiers of subtypes in the process are undefined. Here we used an Id4-eGfp reporter mouse to discover that EGFP intensity is predictive of the subsets with the ID4-EGFP(Bright) population being mostly, if not purely, SSCs while the ID4-EGFP(Dim) population is in transition to the progenitor state...
January 13, 2017: Development
https://www.readbyqxmd.com/read/28077131/granulocyte-colony-stimulating-factor-g-csf-promotes-spermatogenic-regeneration-from-surviving-spermatogonia-after-high-dose-alkylating-chemotherapy
#2
Travis Kotzur, Roberto Benavides-Garcia, Jennifer Mecklenburg, Jamila R Sanchez, Matthew Reilly, Brian P Hermann
BACKGROUND: The lifesaving chemotherapy and radiation treatments that allow patients to survive cancer can also result in a lifetime of side-effects, including male infertility. Infertility in male cancer survivors is thought to primarily result from killing of the spermatogonial stem cells (SSCs) responsible for producing spermatozoa since SSCs turn over slowly and are thereby sensitive to antineoplastic therapies. We previously demonstrated that the cytokine granulocyte colony-stimulating factor (G-CSF) can preserve spermatogenesis after alkylating chemotherapy (busulfan)...
January 11, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28073973/human-foetal-ovary-shares-meiotic-preventing-factors-with-the-developing-testis
#3
Nelly Frydman, Marine Poulain, Brahim Arkoun, Clotilde Duquenne, Sophie Tourpin, Sébastien Messiaen, René Habert, Virginie Rouiller-Fabre, Alexandra Benachi, Gabriel Livera
STUDY QUESTION: How can pre-meiotic germ cells persist in the human foetal ovary? SUMMARY ANSWER: Numerous oogonia escaping meiotic entry were retrieved throughout human ovarian development simultaneously with the expression of signalling pathways preventing meiosis, typically described in the rodent embryonic testis. WHAT IS KNOWN ALREADY: The transition from mitosis to meiosis is a key event in female germ cells that remains poorly documented in research on the human ovary...
January 9, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28071722/spict-a-cyst-cell-specific-gene-regulates-starvation-induced-spermatogonial-cell-death-in-the-drosophila-testis
#4
Ason C-Y Chiang, Heiko Yang, Yukiko M Yamashita
Tissues are maintained in a homeostatic state by balancing the constant loss of old cells with the continued production of new cells. Tissue homeostasis can shift between high and low turnover states to cope with environmental changes such as nutrient availability. Recently, we discovered that the elimination of transit-amplifying cells plays a critical role in maintaining the stem cell population during protein starvation in the Drosophila testis. Here, we identify spict, a gene expressed specifically in differentiating cyst cells, as a regulator of spermatogonial death...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28053620/establishment-of-a-surgically-induced-cryptorchidism-canine-recipient-model-for-spermatogonial-stem-cell-transplantation
#5
Won-Young Lee, Ran Lee, Hyuk Song, Tai-Young Hur, Seunghoon Lee, Jiyun Ahn, Hyunjhung Jhun
Transplantation of spermatogonial stem cells (SSCs) in experimental animal models has been used to study germ line stem cell biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of SSCs and the production of offspring. Here, we investigated the effects of surgically induced cryptorchidism in dog as a new recipient model for spermatogonial stem cell transplantation. Artificially unilateral or bilateral cryptorchidism was induced in ten mature male dogs by surgically returning the testis and epididymis to the abdominal cavity...
December 2016: Laboratory Animal Research
https://www.readbyqxmd.com/read/28045098/effects-of-elevated-%C3%AE-estradiol-levels-on-the-functional-morphology-of-the-testis-new-insights
#6
Myles Leavy, Matthias Trottmann, Bernhard Liedl, Sven Reese, Christian Stief, Benjamin Freitag, John Baugh, Giulio Spagnoli, Sabine Kölle
Elevated estradiol levels are correlated with male infertility. Causes of hyperestrogenism include diseases of the adrenal cortex, testis or medications affecting the hypothalamus-pituitary-gonadal axis. The aim of our study was to elucidate the effects of estradiol treatment on testicular cellular morphology and function, with reference to the treatment regimen received. Testes samples (n = 9) were obtained post-orchiectomy from male-to-female transsexuals within the age range of 26-52 years. Each patient had a minimum of 1-6 years estradiol treatment...
January 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28017656/primary-human-testicular-cells-self-organize-into-organoids-with-testicular-properties
#7
Yoni Baert, Joery De Kock, João P Alves-Lopes, Olle Söder, Jan-Bernd Stukenborg, Ellen Goossens
So far, successful de novo formation of testicular tissue followed by complete spermatogenesis in vitro has been achieved only in rodents. Our findings reveal that primary human testicular cells are able to self-organize into human testicular organoids (TOs), i.e., multi-cellular tissue surrogates, either with or without support of a biological scaffold. Despite lacking testis-specific topography, these mini-tissues harbored spermatogonia and their important niche cells, which retained specific functionalities during long-term culture...
January 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28003832/identification-of-chd1l-as-an-important-regulator-for-spermatogonial-stem-cell-survival-and-self-renewal
#8
Shan-Shan Liu, Yin-Shan Bai, Li Feng, Wen-Wei Dong, Yang Li, Li-Ping Xu, Ning-Fang Ma
Chromodomain helicase/ATPase DNA binding protein 1-like gene (Chd1l) participates in chromatin-dependent processes, including transcriptional activation and DNA repair. In this study, we have found for the first time that Chd1l is mainly expressed in the testicular tissues of prepubertal and adult mice and colocalized with PLZF, OCT4, and GFRα1 in the neonatal mouse testis and THY1(+) undifferentiated spermatogonia or spermatogonial stem cells (SSCs). Knockdown of endogenous Chd1l in cultured mouse undifferentiated SSCs inhibited the expression levels of Oct4, Plzf, Gfrα1, and Pcna genes, suppressed SSC colony formation, and reduced BrdU incorporation, while increasing SSC apoptosis...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27993670/interactome-of-the-inhibitory-isoform-of-the-nuclear-transporter-importin-13
#9
Shadma Fatima, Kylie M Wagstaff, Kim G Lieu, Rebecca G Davies, Satomi S Tanaka, Yasuka L Yamaguchi, Kate L Loveland, Patrick P L Tam, David A Jans
Importin 13 (Imp13) is a bidirectional nuclear transporter of proteins involved in a range of important cellular processes, with an N-terminally truncated inhibitory isoform (tImp13) specifically expressed in testis. To gain insight into tImp13 function, we performed a yeast-2-hybrid screen from a human testis cDNA library, identifying for the first time a suite of interactors with roles in diverse cellular process. We validated the interaction of tImp13 with Eukaryotic translation initiation factor 4γ2 (EIF4G2) and High mobility group containing protein 20A (HMG20A), benchmarking that with glucocorticoid receptor (GR), a known Imp13 interactor expressed in testis...
December 16, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27990704/differentiation-of-bone-marrow-derived-stage-specific-embryonic-antigen-1-positive-pluripotent-stem-cells-into-male-germ-cells
#10
Reza Shirazi, Amir Hassan Zarnani, Masoud Soleimani, Karim Nayernia, Iraj Ragerdi Kashani
Studies published in recent years have changed the outlook on sterility and germ cell development by producing gametes from stem cells. In present study, a novel approach on differentiation of bone marrow-derived stage-specific embryonic antigen 1 positive (SSEA-1(+) ) pluripotent stem cells into male germ cells has been addressed. SSEA-1(+) stem cells were separated from murine bone marrow using magnetic-activated cell sorting (MACS) system and propagated on a feeder layer cells. To evaluate the pluripotency characteristic of the purified cells, they were differentiated toward cells of three germ layers...
December 19, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27927847/preserved-seminiferous-tubule-integrity-with-spermatogonial-survival-and-induction-of-sertoli-and-leydig-cell-maturation-after-long-term-organotypic-culture-of-prepubertal-human-testicular-tissue
#11
F de Michele, J Poels, L Weerens, C Petit, Z Evrard, J Ambroise, D Gruson, C Wyns
STUDY QUESTION: Is an organotypic culture system able to provide the appropriate testicular microenvironment for in-vitro maturation of human immature testicular tissue (ITT)? SUMMARY ANSWER: Our organotypic culture system provided a microenvironment capable of preserving seminiferous tubule (ST) integrity and Leydig cell (LC) functionality and inducing Sertoli cell (SC) maturation. WHAT IS KNOWN ALREADY: Cryopreservation of human ITT is a well-established strategy to preserve fertility in prepubertal boys affected by cancer, with a view for obtaining sperm...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27926879/in%C3%A2-vitro-derivation-and-propagation-of-spermatogonial-stem-cell-activity-from-mouse-pluripotent-stem-cells
#12
Yukiko Ishikura, Yukihiro Yabuta, Hiroshi Ohta, Katsuhiko Hayashi, Tomonori Nakamura, Ikuhiro Okamoto, Takuya Yamamoto, Kazuki Kurimoto, Kenjiro Shirane, Hiroyuki Sasaki, Mitinori Saitou
The in vitro derivation and propagation of spermatogonial stem cells (SSCs) from pluripotent stem cells (PSCs) is a key goal in reproductive science. We show here that when aggregated with embryonic testicular somatic cells (reconstituted testes), primordial germ cell-like cells (PGCLCs) induced from mouse embryonic stem cells differentiate into spermatogonia-like cells in vitro and are expandable as cells that resemble germline stem cells (GSCs), a primary cell line with SSC activity. Remarkably, GSC-like cells (GSCLCs), but not PGCLCs, colonize adult testes and, albeit less effectively than GSCs, contribute to spermatogenesis and fertile offspring...
December 6, 2016: Cell Reports
https://www.readbyqxmd.com/read/27925265/pentoxifylline-besides-naltrexone-recovers-morphine-induced-inflammation-in-male-reproductive-system-of-rats-by-regulating-toll-like-receptor-pathway
#13
S Chehrei, M Moradi, H R Ghiabi, M Falahi, S Kaviani, A Ghanbari
This study aimed to investigate the effect of pentoxifylline on complications of prolonged usage of morphine upon the testis and sperm parameters of rats. In this study, forty male Wistar rats were divided into five groups (n = 8) and treated for 56 days to only saline, only morphine, only pentoxifylline, pentoxifylline + morphine and naltrexone + morphine. The diameters of seminiferous tubules, the maturity of germ line epithelium and sperm parameters were evaluated. The expression of inflammatory-related factors in testis tissues were also investigated at gene and protein levels...
December 7, 2016: Andrologia
https://www.readbyqxmd.com/read/27919445/effective-production-of-recipient-male-pigs-for-spermatogonial-stem-cell-transplantation-by-intratesticular-injection-with-busulfan
#14
Zhuoheng Lin, Jiajing Bao, Qunfang Kong, Yaofu Bai, Fenhua Luo, Zhou Songyang, Yingji Wu, Junjiu Huang
Germ cell transplantation has facilitated spermatogonial stem cell (SSC) and spermatogenesis research and shown great potential in the seed-breeding of domestic livestock. However, little progress has been made in large animals, primarily reflecting the difficulties in preparing sterile recipients. Here, we developed a novel protocol to prepare recipient pigs through the direct injection of busulfan into the cavum vaginale of the scrotums of Landrace-Large bi-crossbreeding male pigs and Seghers male pigs, two economically-important types of pigs, to eliminate endogenous spermatogonia...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/27916459/molecular-characterization-of-sdf1-and-cxcr4-in-the-mozambique-tilapia-oreochromis-mossambicus
#15
Jorge Amat-Fernandez, Michael J Hammond, Di Liang, Tianfang Wang, Tomer Ventura, Abigail Elizur, Scott F Cummins
Animal sexual reproduction relies on primordial germ cells (PGCs), the predecessors of the germ cell lineage, giving rise to either spermatogonia or oogonia after the completion of gonadal differentiation. There is limited information on the mechanism of PGC migration leading to the formation of the primordial gonad in Perciform fish. Oreochromis mossambicus, a tilapiine species, was investigated that is a commercially important aquaculture species in many parts of the world while in other areas it has become an invasive pest...
January 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/27902973/cd147-regulates-extrinsic-apoptosis-in-spermatocytes-by-modulating-nf%C3%AE%C2%BAb-signaling-pathways
#16
Chaoqun Wang, Kin Lam Fok, Zhiming Cai, Hao Chen, Hsiao Chang Chan
CD147 null mutant male mice are infertile with arrested spermatogenesis and increased apoptotic germ cells. Our previous studies have shown that CD147 prevents apoptosis in mouse spermatocytes but not spermatogonia. However, the underlying mechanism remains elusive. In the present study, we aim to determine the CD147-regulated apoptotic pathway in mouse spermatocytes. Our results showed that immunodepletion of CD147 triggered apoptosis through extrinsic apoptotic pathway in mouse testis and spermatocyte cell line (GC-2 cells), accompanied by activation of non-canonical NFκB signaling and suppression of canonical NFκB signaling...
November 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27896558/h3k9ac-involved-in-the-decondensation-of-spermatozoal-nuclei-during-spermatogenesis-in-chinese-mitten-crab-eriocheir-sinensis
#17
Genliang Li, Xianjiang Kang, Shumei Mu, Mingshen Guo, Shiwen Huang, Qinna Chen, Song Nong, Xiaomin Huang, Hongliu Hu, Ke Sun
As a well-known crustacean model species, the Chinese mitten crab Eriocheir sinensis presents spermatozoa with decondensed DNA. Our aim was to analyze structural distribution of the histone H3 and its acetylated lysine 9 (H3K9ac) during spermatogenesis for the mechanistic understanding of the nuclear decondensation of the spermatozoa in E. sinensis. Using specific antibodies, we followed the structural distribution and acetylated lysine 9 of the histone H3 during spermatogenesis, especially spermiogenesis, of E...
November 28, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27890809/germ-cell-responses-to-doxorubicin-exposure-in-vitro
#18
Khaled Habas, Diana Anderson, Martin H Brinkworth
Anthracyclines such as doxorubicin (Dox), widely used to treat various types of tumours, may result in induced testicular toxicity and oxidative stress. The present investigation was designed to determine whether exposure of isolated and purified mouse germ cells to Dox induces DNA damage in the form of strand breaks (presumably) resulting in apoptosis and to investigate the relative sensitivity of specific cell types. DNA damage was assessed using the Comet assay and the presence of apoptosis was determined by TUNEL assay...
January 4, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/27884405/zika-virus-causes-testis-damage-and-leads-to-male-infertility-in-mice
#19
Wenqiang Ma, Shihua Li, Shuoqian Ma, Lina Jia, Fuchun Zhang, Yong Zhang, Jingyuan Zhang, Gary Wong, Shanshan Zhang, Xuancheng Lu, Mei Liu, Jinghua Yan, Wei Li, Chuan Qin, Daishu Han, Chengfeng Qin, Na Wang, Xiangdong Li, George Fu Gao
Zika virus (ZIKV) persists in the semen of male patients, a first for flavivirus infection. Here, we demonstrate that ZIKV can induce inflammation in the testis and epididymidis, but not in the prostate or seminal vesicle, and can lead to damaged testes after 60 days post-infection in mice. ZIKV induces innate immune responses in Leydig, Sertoli, and epididymal epithelial cells, resulting in the production of pro-inflammatory cytokines/chemokines. However, ZIKV does not induce a rapid and abundant cytokine production in peritubular cell and spermatogonia, suggesting that these cells are vulnerable for ZIKV infection and could be the potential repositories for ZIKV...
December 1, 2016: Cell
https://www.readbyqxmd.com/read/27880854/increased-paternal-age-at-conception-is-associated-with-transcriptomic-changes-involved-in-mitochondrial-function-in-elderly-individuals
#20
Tapio Nevalainen, Laura Kananen, Saara Marttila, Juulia Jylhävä, Marja Jylhä, Antti Hervonen, Mikko Hurme
The increased paternal age at conception (PAC) has been associated with autism spectrum disorder (ASD), schizophrenia and other neurodevelopmental disorders, thus raising questions that imply, potential health concerns in the offspring. As opposed to female oogonia, the male germ cells undergo hundreds of cell divisions during the fertile years. Thus, the advanced paternal age is associated with increase of point mutations in the male spermatogonia DNA, implying that this could be the major driving mechanism behind the paternal age effect observed in the offspring...
2016: PloS One
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