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Spermatogonia

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https://www.readbyqxmd.com/read/29904069/exposure-to-an-anti-androgenic-herbicide-negatively-impacts-reproductive-physiology-and-fertility-in-xenopus-tropicalis
#1
F Orton, M Säfholm, E Jansson, Y Carlsson, A Eriksson, J Fick, T Uren Webster, T McMillan, M Leishman, B Verbruggen, T Economou, C R Tyler, C Berg
Amphibians are threatened on a global scale and pollutants may be contributing to population declines, but how chemicals impact on their reproduction is poorly understood. We conducted a life cycle analysis to investigate the impacts of early life exposure to two anti-androgens (exposure until completion of metamorphosis;stage 66): flutamide, (50 µg/L)/linuron (9 and 45 µg/L)) on sexual development and breeding competence in Xenopus tropicalis. Our analyses included: mRNA levels of dmrt1, cyp17, amh, cyp19, foxl2 and ar (tadpoles/metamorphs), gonadal histomorphology (metamorphs/adults), mRNA levels of ar/gr (adult male brain/gonad/forelimb), testosterone/corticosterone levels (adult males), secondary sexual characteristics (forelimb width/nuptial pad: adult males) and breeding competence (amplexus/fertility: adult males)...
June 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29901031/ubiquitous-flame-retardant-toxicants-impair-spermatogenesis-in-a-human-stem-cell-model
#2
Alyse N Steves, Joshua M Bradner, Kristen L Fowler, Danielle Clarkson-Townsend, Brittany J Gill, Adam C Turry, W Michael Caudle, Gary W Miller, Anthony W S Chan, Charles A Easley
Sperm counts have rapidly declined in Western males over the past four decades. This rapid decline remains largely unexplained, but exposure to environmental toxicants provides one potential explanation for this decline. Flame retardants are highly prevalent and persistent in the environment, but many have not been assessed for their effects on human spermatogenesis. Using a human stem cell-based model of spermatogenesis, we evaluated two major flame retardants, hexabromocyclododecane (HBCDD) and tetrabromobisphenol A (TBBPA), under acute conditions simulating occupational-level exposures...
May 25, 2018: iScience
https://www.readbyqxmd.com/read/29896156/analysis-of-endocannabinoid-system-in-rat-testis-during-the-first-spermatogenetic-wave
#3
Marina Migliaccio, Giulia Ricci, Antonio Suglia, Francesco Manfrevola, Ken Mackie, Silvia Fasano, Riccardo Pierantoni, Teresa Chioccarelli, Gilda Cobellis
Endocannabinoids are lipid mediators, enzymatically synthesized and hydrolyzed, that bind cannabinoid receptors. Together with their receptors and metabolic enzymes, they form the "endocannabinoid system" (ECS). Anandamide (AEA) and 2-arachidonoylglycerol (2-AG) are the main endocannabinoids studied in testis. In this study, using the first wave of spermatogenesis as an in vivo model to verify the progressive appearance of germ cells in seminiferous tubules [i.e., spermatogonia, spermatocytes, and spermatids], we analyzed the expression of the main enzymes and receptors of ECS in rat testis...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29895858/a-homozygous-fancm-frameshift-pathogenic-variant-causes-male-infertility
#4
Hao Yin, Hui Ma, Sajjad Hussain, Huan Zhang, Xuefeng Xie, Long Jiang, Xiaohua Jiang, Furhan Iqbal, Ihtisham Bukhari, Hanwei Jiang, Asim Ali, Liangwen Zhong, Tao Li, Suixing Fan, Beibei Zhang, Jianing Gao, Yang Li, Jabeen Nazish, Teka Khan, Manan Khan, Muhammad Zubair, Qiaomei Hao, Hui Fang, Jun Huang, Mahmoud Huleihel, Jiahao Sha, Tej K Pandita, Yuanwei Zhang, Qinghua Shi
PURPOSE: Fanconi anemia (FA) genes play important roles in spermatogenesis. In mice, disruption of Fancm impairs male fertility and testicular integrity, but whether FANCM pathogenic variants (PV) similarly affect fertility in men is unknown. Here we characterize a Pakistani family having three infertile brothers, two manifesting oligoasthenospermia and one exhibiting azoospermia, born to first-cousin parents. A homozygous PV in FANCM (c.1946_1958del, p.P648Lfs*16) was found cosegregating with male infertility...
June 12, 2018: Genetics in Medicine: Official Journal of the American College of Medical Genetics
https://www.readbyqxmd.com/read/29890312/the-characterization-and-potential-roles-of-bone-morphogenetic-protein-7-during-spermatogenesis-in-chinese-mitten-crab-eriocheir-sinensis
#5
Ya-Ru Xu, Gao-Yuan Wang, Yi-Chao Zhou, Wan-Xi Yang
Bone morphogenetic proteins (BMPs), which belong to the transforming growth factor-β superfamily, have been implicated in various biological and physiological processes, especially in the gonad development. However, scarce studies were focused on the roles of BMPs in the reproductive system of crustaceans. In this study, the whole gene encoding BMP7 protein was cloned and characterized firstly in Chinese mitten crab Eriocheir sinensis. The bioinformatics analysis of the deduced amino acid sequence showed that Es-BMP7 was composed of prodomain/latency-associated peptide and the TGF-β characteristic domain...
June 8, 2018: Gene
https://www.readbyqxmd.com/read/29889062/obtaining-and-characterization-of-anti-testis-monoclonal-antibodies-invaluable-tools-toward-the-identification-of-testis-antigens-involved-in-fertilization
#6
Hemati Atefeh, Azarnia Mahnaz, Modaresi Mohammadhosein, Rahimi Amir
BACKGROUND: Generation and utilization of the specific monoclonal antibodies against testis antigens is reported to identify the antigens that are important in reproductive field. OBJECTIVE: Current study aimed to introduce a hybridoma that producing a specific anti-testis monoclonal antibody to identify the testis antigens that can be important in the reproduction field. METHODS: To make hybridoma against testis antigens, mice were immunized with testis cell lysate...
May 25, 2018: Human Antibodies
https://www.readbyqxmd.com/read/29886763/inhibin-b-in-seminiferous-tubules-of-human-testes-in-normal-spermatogenesis-and-in-idiopathic-infertility
#7
Grigory A Demyashkin
Inhibin B is one of the most significant serum markers of spermatogenesis, but its testicular expression has been poorly studied. Inhibin B sensitivity as well as the ability to reflect the level and condition of spermatogenesis and forecast certain changes in reproductive homeostasis in males is still a subject of active discussion. The purpose of this study is to examine the level of expression of inhibin B in cells of human seminiferous tubules in normal and in pathological spermatogenesis (idiopathic infertility) by revealing the proportion of immunostaining cells for inhibin B...
June 9, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29886483/fertility-preservation-in-klinefelter-syndrome-patients-during-the-transition-period
#8
Nathalie Rives, Aurélie Rives, Christine Rondanino, Mireille Castanet, Ariane Cuny, Louis Sibert
Spermatozoa have occasionally been identified in ejaculate of adult Klinefelter syndrome (KS) patients but very exceptionally in KS adolescents. Spermatozoa can also be retrieved in testicular tissue of KS adolescents. The testis may also harbor spermatogonia and noncompletely differentiated germ cells. Neither clinical features nor hormonal parameters could predict germ cell recovery in KS adults or adolescents. No predictive factors can actually demonstrate that early diagnosis of KS would allow increasing the chance of sperm retrieval even if it has been suggested that semen quality may decline with age in KS patients...
2018: Endocrine Development
https://www.readbyqxmd.com/read/29885464/evolutionary-conservation-and-divergence-of-vasa-dazl-and-nanos1-during-embryogenesis-and-gametogenesis-in-dark-sleeper-odontobutis-potamophila
#9
Wenxu Zhu, Tao Wang, Cheng Zhao, Dan Wang, Xinyu Zhang, Hongyan Zhang, Meili Chi, Shaowu Yin, Yongyi Jia
Germline-specific genes, Vasa, Dazl and Nanos1, have highly conserved functions in germline development and fertility across animal phyla. In this study, the full-length sequences of Opvasa, Opdazl and Opnanos1 were cloned and characterized from the dark sleeper (Odontobutis potamophila). Gonad-specific expression patterns of Opvasa and Opdazl were confirmed in adult tissues by quantitative real-time PCR (qRT-PCR). Different from Opvasa and Opdazl, the expression of Opnanos1 was ubiquitously detected in all examined tissues except for the liver and spleen...
June 6, 2018: Gene
https://www.readbyqxmd.com/read/29882505/review-spermatogenesis-in-the-bull
#10
C Staub, L Johnson
Spermatogenesis is a finely regulated process of germ cell multiplication and differentiation leading to the production of spermatozoa in the seminiferous tubules. Spermatogenesis can be divided into three parts: spermatocytogenesis, meiosis and spermiogenesis. During spermatocytogenesis, germ cells engage in a cycle of several mitotic divisions that increases the yield of spermatogenesis and to renew stem cells and produce spermatogonia and primary spermatocytes. Meiosis involves duplication and exchange of genetic material and two cell divisions that reduce the chromosome number and yield four haploid round spermatids...
June 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29849985/isolation-and-proliferation-of-spermatogonial-cells-from-ghezel-sheep
#11
Babak Qasemi-Panahi, Mansoureh Movahedin, Gholamali Moghaddam, Parviz Tajik, Mortaza Koruji, Javad Ashrafi-Helan, Seyed Abbas Rafat
Background: Sheep industry has taken steps toward transforming itself into a more efficient and competitive field. There are many varieties of sheep breeds in the world that each of them serves a useful purpose in the economies of different civilizations. Ghezel sheep is one of the Iranian important breeds that are raised for meat, milk and wool. Field of spermatogonial cell technologies provides tools for genetic improvement of sheep herd and multiple opportunities for research. Spermatogonial cells are the only stem cells capable of transmitting genetic information to future generations...
April 2018: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/29842884/novel-localization-of-aurora-a-kinase-in-mouse-testis-suggests-multiple-roles-in-spermatogenesis
#12
Marquita L Johnson, Rong Wang, Ann O Sperry
Male germ cells are transformed from undifferentiated stem cells into spermatozoa through a series of highly regulated steps together termed spermatogenesis. Spermatogonial stem cells undergo mitosis and differentiation followed by two rounds of meiotic division and then proceed through a series of dramatic cell shape changes to form highly differentiated spermatozoa. Using indirect immunofluorescence, we investigated a role for the mitotic kinase, Aurora A (AURKA) in these events through localization of this protein in mouse testis and spermatozoa...
May 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29805545/effect-of-aromatase-inhibitor-letrozole-on-the-proliferation-of-spermatogonia-by-regulating-the-mapk-pathway
#13
Shunde Wang, Shuhong Wang, Hang Li, Xiaoxia Li, Menglin Xie, Jiayu Wen, Meicai Li, Tengbo Long
The molecular mechanism of the aromatase inhibitor letrozole was investigated. It promotes the proliferation of spermatogonia by regulating the mitogen-activated protein kinase (MAPK) pathway. Six different concentrations were selected for letrozole in order to incubate mouse spermatogonia [GC-1 spermatogonia (spg)] for 24, 48 and 72 h, respectively. Cell Counting Kit-8 (CCK-8) was used to observe the effect of letrozole on the proliferation of GC-1 spg cells, and the effect was further verified by cell plate clone formation assay...
June 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29794024/reproductive-performance-of-male-mice-after-hypothalamic-ghrelin-administration
#14
Maria Belen Poretti, Camila Frautschi, Eugenia Mercedes Luque, Santiago Bianconi, Ana Carolina Martini, Graciela Stutz, Laura Maria Vincenti, María Emilia Santillán, Marina Flavia Ponzio, Helgi Schiöth, Marta Haydee Fiol De Cuneo, Valeria Paola Carlini
It has been demonstrated that food intake and reproductive physiology are both simultaneously modulated to optimize reproductive success under fluctuating metabolic conditions. Ghrelin (Ghr) is an orexigenic peptide identified as the endogenous ligand of the growth hormone secretagogue receptor that is being investigated for its potential role on reproduction. Considering that data available so far are still limited and characterization of Ghr action mechanism on the reproductive system has not been fully elucidated, we studied the hypothalamus participation in Ghr effects on sperm functional activity, plasma levels of gonodotropins and histological morphology in mice testes after hypothalamic infusion of 0...
May 23, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29789776/effect-of-electromagnetic-waves-from-mobile-phones-on-spermatogenesis-in-the-era-of-4g-lte
#15
Jong Jin Oh, Seok-Soo Byun, Sang Eun Lee, Gheeyoung Choe, Sung Kyu Hong
Objective: To investigate the effect of long duration exposure to electromagnetic field from mobile phones on spermatogenesis in rats using 4G-LTE. Methods: Twenty Sprague-Dawley male rats were placed into 4 groups according to the intensity and exposure duration: Group 1 (sham procedure), Group 2 (3 cm distance + 6 h exposure daily), Group 3 (10 cm distance + 18 h exposure daily), and Group 4 (3 cm distance + 18 h exposure daily). After 1 month, we compared sperm parameters and histopathological findings of the testis...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29788325/ancestral-tcdd-exposure-induces-multigenerational-histologic-and-transcriptomic-alterations-in-gonads-of-male-zebrafish
#16
Danielle N Meyer, Bridget B Baker, Tracie R Baker
TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin), the classic AhR (aryl hydrocarbon receptor) agonist, is a potent environmental toxicant and endocrine-disrupting chemical (EDC) with known developmental toxicity in humans, rodents, and fish. Early life exposure to some EDCs, including TCDD, is linked to the occurrence of adult-onset and multigenerational disease. Previous work exposing juvenile F0 zebrafish (Danio rerio) to 50 ppt (parts per trillion) TCDD during reproductive development has shown male-mediated transgenerational decreases in fertility (F0-F2) and histological and transcriptomic alterations in F0 testes...
May 21, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29781095/gasoline-exhaust-damages-spermatogenesis-through-downregulating-%C3%AE-6-integrin-and-%C3%AE-1-integrin-in-the-rat-model
#17
L Luo, E Li, S Zhao, J Wang, Z Zhu, Y Liu, J Luo, Z Zhao
The health influence of air pollution has been an international public health concern. Increasing evidence has suggested that air pollution has been associated with decreased sperm quality. However, the underlying molecular mechanisms are still not fully elucidated. We aimed to verify whether gasoline exhaust leads to reproductive impairment by injuring spermatogonial stem cells and explore its underlying molecular mechanism. Twenty male Sprague-Dawley rats were randomly divided into two groups: the exposure group (n = 10) and the control group (n = 10)...
May 20, 2018: Andrologia
https://www.readbyqxmd.com/read/29772210/in-silico-analysis-seasonal-variation-and-gonadotropic-regulation-of-jag1-and-its-receptor-notch1-in-testis-of-spotted-snakehead-channa-punctatus
#18
Alivia Roy, Reetuparna Basak, Umesh Rai
The present study in seasonally breeding spotted snakehead Channa punctatus, for the first time in nonmammalian vertebrates, demonstrated correlation between reproductive phase-dependent testicular expression of ligand Jag1/receptor Notch1 and spermatogenic events. Testicular transcriptome sequencing data from our earlier study in C. punctatus was used in the present study to select the best transcript for jag1 (cpjag1) and notch1 (cpnotch1). The transcripts cpjag1 and cpnotch1 encoded full-length putative proteins of 1215 (cpJag1) and 2475 (cpNotch1) amino acids, respectively...
May 14, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29769589/conserved-and-non-conserved-characteristics-of-porcine-glial-cell-line-derived-neurotrophic-factor-expressed-in-the-testis
#19
Kazue Kakiuchi, Kazumi Taniguchi, Hiroshi Kubota
Glial cell line-derived neurotrophic factor (GDNF) is essential for the self-renewal and proliferation of spermatogonial stem cells (SSCs) in mice, rats, and rabbits. Although the key extrinsic factors essential for spermatogonial proliferation in other mammals have not been determined, GDNF is one of the potential candidates. In this study, we isolated porcine GDNF (pGDNF) cDNAs from neonatal testis and generated recombinant pGDNF to investigate its biological activity on gonocytes/undifferentiated spermatogonia, including SSCs...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769570/in-utero-and-postnatal-exposure-to-high-fat-high-sucrose-diet-suppressed-testis-apoptosis-and-reduced-sperm-count
#20
Jiude Mao, Kathleen A Pennington, Omonseigho O Talton, Laura C Schulz, Miriam Sutovsky, Yan Lin, Peter Sutovsky
Obesity affects male fertility and maternal diabetes affects the offspring sperm epigenome. However, the effects of in utero exposure to maternal glucose intolerance in combination with postnatal high fat, high sucrose (HFHS) diet consumption on offspring spermatogenesis is not clear. The present study was designed to test these effects. One week before and during pregnancy, dams were fed either control or HFHS diet to induce gestational glucose intolerance, and returned to standard diet during lactation. Male offspring from each maternal group were split into control and HFHS-fed groups for eight weeks prior to sacrifice at 11, 19 or 31 weeks of age, and reproductive tissues were harvested for analysis of testicular germ cell apoptosis and sperm output...
May 16, 2018: Scientific Reports
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