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https://www.readbyqxmd.com/read/28645700/antagonistic-regulation-of-spermatogonial-differentiation-in-zebrafish-danio-rerio-by-igf3-and-amh
#1
R D V S Morais, D Crespo, R H Nóbrega, M S Lemos, H J G van de Kant, L R de França, R Male, J Bogerd, R W Schulz
Fsh-mediated regulation of zebrafish spermatogenesis includes modulating the expression of testicular growth factors. Here, we study if and how two Sertoli cell-derived Fsh-responsive growth factors, anti-Müllerian hormone (Amh; inhibiting steroidogenesis and germ cell differentiation) and insulin-like growth factor 3 (Igf3; stimulating germ cell differentiation), cooperate in regulating spermatogonial development. In dose response and time course experiments with primary testis tissue cultures, Fsh up-regulated igf3 transcript levels and down-regulated amh transcript levels; igf3 transcript levels were more rapidly up-regulated and responded to lower Fsh concentrations than were required to decrease amh mRNA levels...
June 20, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28644726/cytotoxic-and-genotoxic-actions-of-casiopeina-iii-ea-cas-iii-ea-in-somatic-and-germ-cells-of-drosophila-melanogaster
#2
L M Vidal, E Pimentel, M P Cruces, S Hernández, L Ruiz-Azuara
Casiopeinas® are a group of newly synthesized drugs designed to treat cancer. These copper (Cu) complexes exhibit cytostatic, cytotoxic, genotoxic, and antineoplastic activities through different mechanisms of action. To evaluate the influence of these compounds, some in vivo studies were performed using predominantly somatic cells. The aim of the present study was to examine the cytotoxic and genotoxic actions of Casiopeina III-Ea (Cas III-Ea) in somatic as well as germ cells of Drosophila melanogaster. For cytotoxicity, the productivity and some morphometric parameters were measured and genotoxicity was assessed by means of the somatic mutation and recombination test assay in the wing...
June 23, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28629181/knockout-of-murine-mamld1-impairs-testicular-growth-and-daily-sperm-production-but-permits-normal-postnatal-androgen-production-and-fertility
#3
Mami Miyado, Kaoru Yoshida, Kenji Miyado, Momori Katsumi, Kazuki Saito, Shigeru Nakamura, Tsutomu Ogata, Maki Fukami
MAMLD1 has been implicated in testicular function in both human and mouse fetuses. Although three patients with MAMLD1 mutations were reported to have hypergonadotropic hypogonadism in their teens, the functional significance of MAMLD1 in the postnatal testis remains unclear. Here, we analyzed the phenotype of Mamld1 knockout (KO) male mice at reproductive ages. The reproductive organs of KO male mice were morphologically unremarkable, except for relatively small testes. Seminiferous tubule size and number of proliferating spermatogonia/spermatocytes were reduced in the KO testis...
June 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28624768/a-revised-asingle-model-to-explain-stem-cell-dynamics-in-the-mouse-male-germline
#4
REVIEW
Tessa Lord, Jon M Oatley
Spermatogonial stem cells (SSCs) and progenitor spermatogonia encompass the undifferentiated spermatogonial pool in mammalian testes. In rodents, this population is comprised of Asingle, Apaired and chains of 4-16 Aaligned spermatogonia. Although traditional models propose that the entire Asingle pool represents SSCs, and formation of an Apaired syncytium symbolizes irreversible entry to a progenitor state destined for differentiation; recent models have emerged that suggest that the Asingle pool is heterogeneous, and Apaired/Aaligned can fragment to produce new SSCs...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28615530/in-vivo-autophagy-and-biogenesis-of-autophagosomes-within-male-haploid-cells-during-spermiogenesis
#5
Ping Yang, Nisar Ahmed, Lingling Wang, Hong Chen, Yasir Waqas, Tengfei Liu, Abdul Haseeb, Nasrullah Bangulzai, Yufei Huang, Qiusheng Chen
Autophagy is a unique catabolic pathway that is linked to several physiological processes. However, its role in the process of spermiogenesis is largely unknown. The aim of the current study was to determine the in vivo role of autophagy and the origin of autophagosome membrane biogenesis within male haploid cells. Our immunohistochemistry results demonstrated that LC3 and ATG7 localization were increased dramatically in round to elongated spermatids (haploid cells) towards the lumen of seminiferous tubules, however, poorly expressed in the early stages of germ cells near the basal membrane...
May 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28611311/genome-wide-5-hydroxymethylcytosine-patterns-in-human-spermatogenesis-are-associated-with-semen-quality
#6
Olga A Efimova, Anna A Pendina, Andrei V Tikhonov, Sergey E Parfenyev, Irina D Mekina, Evgeniia M Komarova, Mariia A Mazilina, Eugene V Daev, Olga G Chiryaeva, Ilona A Galembo, Mikhail I Krapivin, Oleg S Glotov, Irina S Stepanova, Svetlana A Shlykova, Igor Yu. Kogan, Alexander M Gzgzyan, Tatyana V Kuznetzova, Vladislav S Baranov
We performed immunofluorescent analysis of DNA hydroxymethylation and methylation in human testicular spermatogenic cells from azoospermic patients and ejaculated spermatozoa from sperm donors and patients from infertile couples. In contrast to methylation which was present throughout spermatogenesis, hydroxymethylation was either high or almost undetectable in both spermatogenic cells and ejaculated spermatozoa. On testicular cytogenetic preparations, 5-hydroxymethylcytosine was undetectable in mitotic and meiotic chromosomes, and was present exclusively in interphase spermatogonia Ad and in a minor spermatid population...
June 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28611113/active-paraoxonase-1-is-synthesised-throughout-the-internal-boar-genital-organs
#7
Isabel Barranco, Cristina Perez-Patiño, Asta Tvarijonaviciute, Inmaculada Parrilla, Alejandro Vicente-Carrillo, Manuel Alvarez-Rodriguez, Jose J Ceron, Emilio Martinez, Heriberto Rodriguez-Martinez, Jordi Roca
The paraoxonase type 1 (PON1) is an enzyme with antioxidant properties recently identified in the seminal plasma (SP) of several species, including the porcine. The aims of the present study were to (1) describe the inmunohistochemical localization of PON1 in the genital organs of fertile boars and (2) evaluate the relationship among PON1 activity and high-density lipoprotein cholesterol (HDL-C) concentration in fluids of the boar genital organs. Immunohistochemical analysis demonstrated that PON1 was present in testis (specifically in Leydig cells, blood vessels, spermatogonia and elongated spermatids), epididymis (specifically in the cytoplasm of the principal epithelial cells, luminal secretion and in the surrounding smooth muscle) and the lining epithelia of the accessory sexual glands (cytoplasmic location in the prostate and membranous in the seminal vesicle and bulbourethral glands)...
June 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28608322/long-evans-rat-spermatogonial-lines-are-effective-germline-vectors-for-transgenic-rat-production
#8
F Kent Hamra, Christopher T Richie, Brandon K Harvey
Long Evans rat strains are applied as research models in a broad spectrum of biomedical fields (>15,800 citations, NCBI PubMed). Here, we report an approach to genetically modify the Long Evans rat germline in donor spermatogonial stem cells. Long Evans rat spermatogonial lines were derived from freshly isolated laminin-binding spermatogonia. Laminin-binding spermatogonia were cultured over multiple passages on fibroblast feeder layers in serum-free culture medium containing GDNF and FGF2. Long Evans rat spermatogonial lines were genetically modified by transposon transduction to express a germline, tdTomato reporter gene...
June 12, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28600885/immunolocalization-of-tar-dna-binding-protein-of-43%C3%A2-kda-tdp-43-in-mouse-seminiferous-epithelium
#9
Hari Prasad Osuru, Patcharin Pramoonjago, Mayuresh Abhyankar, Eric Swanson, LaToya Ann Roker, Helen Cathro, Prabhakara P Reddi
TDP-43 (TAR DNA-binding protein of 43 kDa) is an evolutionarily conserved, ubiquitously expressed, multi-functional DNA/RNA-binding protein with roles in gene transcription, mRNA splicing, stability, transport, micro RNA biogenesis, and suppression of transposons. Aberrant expression of TDP-43 in testis and sperm was recently shown to be associated with male infertility, which highlights the need to understand better the expression of TDP-43 in the testis. We previously cloned TDP-43 from a mouse testis cDNA library, and showed that it functions as a transcriptional repressor, and regulates the precise spatiotemporal expression of the Acrv1 gene, which encodes the acrosomal protein SP-10, during spermatogenesis...
June 10, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28585553/nedd4-controls-spermatogonial-stem-cell-homeostasis-and-stress-response-by-regulating-messenger-ribonucleoprotein-complexes
#10
Zhi Zhou, Hiroshi Kawabe, Atsushi Suzuki, Kaori Shinmyozu, Yumiko Saga
P bodies (PBs) and stress granules (SGs) are conserved cytoplasmic aggregates of cellular messenger ribonucleoprotein complexes (mRNPs) that are implicated in mRNA metabolism and play crucial roles in adult stem cell homeostasis and stress responses. However, the mechanisms underlying the dynamics of mRNP granules are poorly understood. Here, we report NEDD4, an E3 ubiquitin ligase, as a key regulator of mRNP dynamics that controls the size of the spermatogonial progenitor cell (SPC) pool. We find that NEDD4 targets an RNA-binding protein, NANOS2, in spermatogonia to destabilize it, leading to cell differentiation...
June 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/28583375/short-term-storage-of-human-testicular-tissue-effect-of-storage-temperature-and-tissue-size
#11
Katrien Faes, Ellen Goossens
During short-term storage, before cryopreservation, testicular tissue quality can be affected by storage medium, duration, temperature and tissue size. We previously established the best storage medium and time for short-term maintenance of tissue. In this study, three different storage temperatures (4°C, room temperature, 37°C) and four tissue sizes (~6 mm(3); ~ 15 mm(3); ~ 50 mm(3) or ~80 mm(3)) were assessed over the course of a fixed period of 3 days. Storing human testicular tissue at 37°C caused a significant increase in the number of apoptotic cells per tubule (P = 0...
May 22, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28583077/identification-of-conserved-genes-triggering-puberty-in-european-sea-bass-males-dicentrarchus-labrax-by-microarray-expression-profiling
#12
Mercedes Blázquez, Paula Medina, Berta Crespo, Ana Gómez, Silvia Zanuy
BACKGROUND: Spermatogenesis is a complex process characterized by the activation and/or repression of a number of genes in a spatio-temporal manner. Pubertal development in males starts with the onset of the first spermatogenesis and implies the division of primary spermatogonia and their subsequent entry into meiosis. This study is aimed at the characterization of genes involved in the onset of puberty in European sea bass, and constitutes the first transcriptomic approach focused on meiosis in this species...
June 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28575466/dose-response-mutation-and-spectrum-analyses-reveal-similar-responses-at-two-microsatellite-loci-in-benzo-a-pyrene-exposed-mouse-spermatogonia
#13
Andrea Rowan-Carroll, Marc A Beal, Andrew Williams, Francesco Marchetti, Carole L Yauk
Identifying chemical exposures that can cause germline mutations is important as these mutations can be inherited, impacting both individual and population health. However, germline mutations are extremely rare and difficult to detect. Chemically induced germline mutations can be detected through analysis of highly unstable tandem repeat DNA. We recently developed a single-molecule PCR (SM-PCR) approach to quantify mutations at a mouse microsatellite locus (Mm2.2.1) in sperm for such purposes. In this study, we refine this approach through the combined analysis of mouse microsatellites Mm2...
May 30, 2017: Mutagenesis
https://www.readbyqxmd.com/read/28575233/stem-cell-activity-of-type-a-spermatogonia-is-seasonally-regulated-in-rainbow-trout
#14
Mana Sato, Makoto Hayashi, Goro Yoshizaki
Spermatogonial stem cells (SSCs) support continuous production of sperm throughout the male's life. However, the biological characteristics of SSCs are poorly understood in animals exhibiting seasonal reproduction, even though most wild animals are seasonal breeders. During the spermiation season in rainbow trout, the lumen of the testes contain only spermatozoa and scattered type A spermatogonia (ASG) along the walls of the testicular lobules. These few remaining ASG, designated "residual ASG," are the only germ cells capable of supporting the next spermatogenesis, suggesting that the residual ASG are true SSCs...
May 30, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28569396/mir-100-promotes-the-proliferation-of-spermatogonial-stem-cells-via-regulating-stat3
#15
Yong-Li Huang, Guan-You Huang, Jing Lv, Li-Na Pan, Xi Luo, Jie Shen
Micro RNAs play important roles during mammalian spermatogenesis, but the function(s) of specific miRNAs remain largely unknown. Here, we report that miR-100 is predominantly expressed in undifferentiated murine spermatogonia, including spermatogonial stem cells (SSCs). We utilized a miRNA mimic and inhibitor to knock down and overexpress miR-100 in cultured SSCs, respectively, finding that the miR-100 promotes the proliferation of SSCs in vitro. Furthermore, signals promoting SSC maintenance induced, whereas retinoic acid repressed, expression of miR-100...
June 1, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28559472/spermatogonial-deubiquitinase-usp9x-is-essential-for-proper-spermatogenesis-in-mice
#16
Kasane Kishi, Aya Uchida, Hinako M Takase, Hitomi Suzuki, Masamichi Kurohmaru, Naoki Tsunekawa, Masami Kanai-Azuma, Stephen A Wood, Yoshiakira Kanai
USP9X (ubiquitin-specific peptidase 9, X chromosome) is the mammalian orthologue of Drosophila deubiquitinase fat facets that was previously shown to regulate the maintenance of the germ cell lineage partially through stabilizing Vasa, one of the widely conserved factors crucial for gametogenesis. Here, we demonstrate that USP9X is expressed in the gonocytes and spermatogonia in mouse testes from newborn to adult stages. By using Vasa-Cre mice, germ cell-specific conditional deletion of Usp9x from the embryonic stage showed no abnormality in the developing testes by 1 week and no appreciable defects in the undifferentiated and differentiating spermatogonia at postnatal and adult stages...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28543866/testicular-structure-and-seminal-pathway-in-the-yellowtail-tetra-astyanax-altiparanae-characiforms-characidae
#17
D H de Siqueira-Silva, A P Dos Santos-Silva, C Bashiyo-Silva, M S Rodrigues, C A Vicentini, A Ninhaus-Silveira, R Veríssimo-Silveira
This study aimed to describe testicular and its main ducts structure in the yellowtail tetra Astyanax altiparanae, contributing to the knowledge of the region in which semen is produced, storage and released, focusing mainly on the dynamic of germinal epithelium and Sertoli cells during germ cell maturation. Ten sexually mature male A. altiparanae had their testes processed according to the routine protocols to optical microscopy. Moreover, spermatic ducts and tubular compartment of the testes of three specimens were perfused with vinyl resin for gross anatomy and scanning electron microscopy...
May 22, 2017: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/28542371/whole-genome-sequencing-of-spermatocytic-tumors-provides-insights-into-the-mutational-processes-operating-in-the-male-germline
#18
Eleni Giannoulatou, Geoffrey J Maher, Zhihao Ding, Ad J M Gillis, Lambert C J Dorssers, Alexander Hoischen, Ewa Rajpert-De Meyts, Gilean McVean, Andrew O M Wilkie, Leendert H J Looijenga, Anne Goriely
Adult male germline stem cells (spermatogonia) proliferate by mitosis and, after puberty, generate spermatocytes that undertake meiosis to produce haploid spermatozoa. Germ cells are under evolutionary constraint to curtail mutations and maintain genome integrity. Despite constant turnover, spermatogonia very rarely form tumors, so-called spermatocytic tumors (SpT). In line with the previous identification of FGFR3 and HRAS selfish mutations in a subset of cases, candidate gene screening of 29 SpTs identified an oncogenic NRAS mutation in two cases...
2017: PloS One
https://www.readbyqxmd.com/read/28534180/the-c-terminal-kinesin-motor-kifc1-may-participate-in-nuclear-reshaping-and-flagellum-formation-during-spermiogenesis-of-larimichthys-crocea
#19
Dan-Dan Zhang, Xin-Ming Gao, Yong-Qiang Zhao, Cong-Cong Hou, Jun-Quan Zhu
Spermatogenesis is a highly ordered process in the differentiation of male germ cells. Nuclear morphogenesis is one of the most fundamental cellular transformations to take place during spermatogenesis. These striking transformations from spermatogonia to spermatozoa are a result of phase-specific adaption of the cytoskeleton and its association with molecular motor proteins. KIFC1 is a C-terminal kinesin motor protein that plays an essential role in acrosome formation and nuclear reshaping during spermiogenesis in mammals...
May 23, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28532845/characterization-of-undifferentiated-spermatogonia-and-the-spermatogonial-niche-in-the-lambari-fish-astyanax-altiparanae
#20
Marília de Paiva Camargo, Mônica Cassel, Lázaro Wender Oliveira de Jesus, Rafael Henrique Nóbrega, Maria Inês Borella
Undifferentiated type A spermatogonia are the foundation of fish spermatogenesis. This cell population includes the spermatogonial stem cell population, which is able to either self-renew or differentiate into cells that will generate the male gamete - the spermatozoa. Spermatogonia stem cells are located in a specific region of the testes known as the spermatogonial niche, which regulates spermatogonial stem cell function. This study characterizes undifferentiated type A spermatogonia and their S-phase label-retaining cell properties in Astyanax altiparanae testes...
July 1, 2017: Theriogenology
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