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Spermatogonia

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https://www.readbyqxmd.com/read/28708521/flow-cytometric-enrichment-of-pacific-bluefin-tuna-type-a-spermatogonia-based-on-light-scattering-properties
#1
REVIEW
Kensuke Ichida, Kazuyoshi Kise, Tetsuro Morita, Ryosuke Yazawa, Yutaka Takeuchi, Goro Yoshizaki
We previously established surrogate broodstock in which the donor germ cells transplanted into the peritoneal cavities of xenogeneic recipients were capable of developing into functional eggs and sperm in teleost fish. In this transplantation system, only the undifferentiated germ cells such as type A spermatogonia (ASG) or a portion of the ASG population were capable of being incorporated into the genital ridges of the recipients and undergo gametogenesis. Therefore, the use of enriched ASGs can be expected to achieve efficient donor-cell incorporation...
June 22, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708515/stage-specific-expression-of-sal-like-protein-4-in-boar-testicular-germ-cells
#2
Hyun-Jung Park, Ran Lee, Won-Young Lee, Jin-Hoi Kim, Jeong Tae Do, Chankyu Park, Hyuk Song
Spermatogenesis, the complex process of sperm cell development including mitotic cell division and meiosis, relies on spermatogonial stem cells (SSCs). While markers for developing germ cells have been well investigated in mice, developmental stage-specific markers of germ cells in domestic animals have not been identified. Sal-like protein 4 (SALL4) is known as a putative marker for undifferentiated spermatogonia in rodents; however, its expression in domestic animals has not been investigated. The objective of this study was to characterize the expression of SALL4 in the developmental stages of boar testes and SSCs...
June 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/28694759/using-curcumin-to-prevent-structural-impairments-of-testicles-in-rats-induced-by-sodium-metabisulfite
#3
Reza Mahmoudi, Zahra Honarmand, Saied Karbalay-Doust, Mehrzad Jafari-Barmak, Mohsen Nikseresht, Ali Noorafshan
Sodium metabisulfite (Na-MBS) is a disinfectant and preservative agent. Some organ including testicle would be in danger in the case of Na-MBS consumption. Curcumin (CUR) is the constituent of turmeric with protective properties. The effect of CUR on testicles in rats exposed to Na-MBS evaluated using stereological methods. Sprague-Dawley rats were divided into eight groups. The rats in groups I to VIII received the following respectively: distilled water, CUR (100 mg/kg/day), low (0.7 mg/kg/day: acceptable daily intake), intermediate (7 mg/ kg/day), and high (70 mg/kg/day) doses of Na-MBS, and low, intermediate, and high doses of Na-MBS plus CUR...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28693937/spermatogenic-dynamics-of-the-spiny-rat-kannabateomys-amblyonyx-wagner-1845-rodentia-echimyidae
#4
Verônica A Siman, Raquel S M Godoy, Fernanda C R Dias, Fabiano A Silva, Gisele M L Del Giudice, Marcos L M Gomes, Sérgio L P Matta
Rodents are distributed worldwide, playing important ecological roles regarding preservation of forest areas. Thus, the study of their reproductive biology is a key for conservation initiatives that prevent extinction and/or improve species management. The present study aimed to describe the spermatogenic dynamics of the spiny rat Kannabateomys amblyonyx, an endemic species from Atlantic Rainforest areas, in Brazil. The average body weight was 418.43g with a gonadosomatic index of 0.41%. The testicular parenchyma organization followed the pattern described for other rodent species, with a large amount of seminiferous tubules occupying 93...
June 28, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28690291/geminin-deletion-in-pre-meiotic-dna-replication-stage-causes-spermatogenesis-defect-and-infertility
#5
Yue Yuan, Xue-Shan Ma, Qiu-Xia Liang, Zhao-Yang Xu, Lin Huang, Tie-Gang Meng, Fei Lin, Heide Schatten, Zhen-Bo Wang, Qing-Yuan Sun
Geminin plays a critical role in cell cycle regulation by regulating DNA replication and serves as a transcriptional molecular switch that directs cell fate decisions. Spermatogonia lacking Geminin disappear during the initial wave of mitotic proliferation, while geminin is not required for meiotic progression of spermatocytes. It is unclear whether geminin plays a role in pre-meiotic DNA replication in later-stage spermatogonia and their subsequent differentiation. Here, we selectively disrupted Geminin in the male germline using the Stra8-Cre/loxP conditional knockout system...
July 9, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28686696/morphological-and-transcriptomic-evidence-for-ammonium-induction-of-sexual-reproduction-in-thalassiosira-pseudonana-and-other-centric-diatoms
#6
Eric R Moore, Briana S Bullington, Alexandra J Weisberg, Yuan Jiang, Jeff Chang, Kimberly H Halsey
The reproductive strategy of diatoms includes asexual and sexual phases, but in many species, including the model centric diatom Thalassiosira pseudonana, sexual reproduction has never been observed. Furthermore, the environmental factors that trigger sexual reproduction in diatoms are not understood. Although genome sequences of a few diatoms are available, little is known about the molecular basis for sexual reproduction. Here we show that ammonium reliably induces the key sexual morphologies, including oogonia, auxospores, and spermatogonia, in two strains of T...
2017: PloS One
https://www.readbyqxmd.com/read/28669602/the-bile-acid-nuclear-receptor-fxr%C3%AE-is-a-critical-regulator-of-mouse-germ-cell-fate
#7
Emmanuelle Martinot, Lauriane Sèdes, Marine Baptissart, Hélène Holota, Betty Rouaisnel, Christelle Damon-Soubeyrand, Angélique De Haze, Jean-Paul Saru, Christelle Thibault-Carpentier, Céline Keime, Jean-Marc A Lobaccaro, Silvère Baron, Gérard Benoit, Françoise Caira, Claude Beaudoin, David H Volle
Spermatogenesis is the process by which spermatozoa are generated from spermatogonia. This cell population is heterogeneous, with self-renewing spermatogonial stem cells (SSCs) and progenitor spermatogonia that will continue on a path of differentiation. Only SSCs have the ability to regenerate and sustain spermatogenesis. This makes the testis a good model to investigate stem cell biology. The Farnesoid X Receptor alpha (FXRα) was recently shown to be expressed in the testis. However, its global impact on germ cell homeostasis has not yet been studied...
July 11, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28661483/gene-knockout-of-zmym3-in-mice-arrests-spermatogenesis-at-meiotic-metaphase-with-defects-in-spindle-assembly-checkpoint
#8
Xiangjing Hu, Bin Shen, Shangying Liao, Yan Ning, Longfei Ma, Jian Chen, Xiwen Lin, Daoqin Zhang, Zhen Li, Chunwei Zheng, Yanmin Feng, Xingxu Huang, Chunsheng Han
ZMYM3, a member of the MYM-type zinc finger protein family and a component of a LSD1-containing transcription repressor complex, is predominantly expressed in the mouse brain and testis. Here, we show that ZMYM3 in the mouse testis is expressed in somatic cells and germ cells until pachytene spermatocytes. Knockout (KO) of Zmym3 in mice using the CRISPR-Cas9 system resulted in adult male infertility. Spermatogenesis of the KO mice was arrested at the metaphase of the first meiotic division (MI). ZMYM3 co-immunoprecipitated with LSD1 in spermatogonial stem cells, but its KO did not change the levels of LSD1 or H3K4me1/2 or H3K9me2...
June 29, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28656658/pl10-dead-box-protein-is-expressed-during-germ-cell-differentiation-in-the-reptile-podarcis-sicula-family-lacertidae
#9
Liliana Milani, Andrea Pecci, Carmine Cifaldi, Maria Gabriella Maurizii
Among genes involved in the regulation of germ cell differentiation, those of DDX4/Vasa and the Ded1/DDX3 subfamilies encode for DEAD-box ATP-dependent RNA helicases, proteins involved in many mechanisms related to RNA processing. For the first time in reptiles, using specific antibodies at confocal microscopy, we analysed the localization pattern of a Ded1/DDX3 subfamily member in testis and ovary of Podarcis sicula (Ps-PL10) during the reproductive cycle. In testis, Ps-PL10 is expressed in the cytoplasm of spermatocytes and it is not detected in spermatogonia...
July 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28652931/piwi1-is-essential-for-gametogenesis-in-mollusk-chlamys-farreri
#10
Xiaoshi Ma, Aichang Ji, Zhifeng Zhang, Dandan Yang, Shaoshuai Liang, Yuhan Wang, Zhenkui Qin
Piwi (P-element induced wimpy testis) is an important gene involved in stem cell maintenance and gametogenesis in vertebrates. However, in most invertebrates, especially mollusks, the function of Piwi during gametogenesis remains largely unclear. To further understand the function of Piwi during gametogenesis, full-length cDNA of Piwi1 from scallop Chlamys farreri (Cf-Piwi1) was characterized, which consisted of a 2,637 bp open reading frame encoding an 878-amino acid protein. Cf-Piwi1 mRNA was mainly localized in the spermatogonia, spermatocytes, oogonia, oocytes of early development and intra-gonadal somatic cells...
2017: PeerJ
https://www.readbyqxmd.com/read/28645700/antagonistic-regulation-of-spermatogonial-differentiation-in-zebrafish-danio-rerio-by-igf3-and-amh
#11
R D V S Morais, D Crespo, R H Nóbrega, M S Lemos, H J G van de Kant, L R de França, R Male, J Bogerd, R W Schulz
Fsh-mediated regulation of zebrafish spermatogenesis includes modulating the expression of testicular growth factors. Here, we study if and how two Sertoli cell-derived Fsh-responsive growth factors, anti-Müllerian hormone (Amh; inhibiting steroidogenesis and germ cell differentiation) and insulin-like growth factor 3 (Igf3; stimulating germ cell differentiation), cooperate in regulating spermatogonial development. In dose response and time course experiments with primary testis tissue cultures, Fsh up-regulated igf3 transcript levels and down-regulated amh transcript levels; igf3 transcript levels were more rapidly up-regulated and responded to lower Fsh concentrations than were required to decrease amh mRNA levels...
June 20, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28644726/cytotoxic-and-genotoxic-actions-of-casiopeina-iii-ea-cas-iii-ea-in-somatic-and-germ-cells-of-drosophila-melanogaster
#12
L M Vidal, E Pimentel, M P Cruces, S Hernández, L Ruiz-Azuara
Casiopeinas® are a group of newly synthesized drugs designed to treat cancer. These copper (Cu) complexes exhibit cytostatic, cytotoxic, genotoxic, and antineoplastic activities through different mechanisms of action. To evaluate the influence of these compounds, some in vivo studies were performed using predominantly somatic cells. The aim of the present study was to examine the cytotoxic and genotoxic actions of Casiopeina III-Ea (Cas III-Ea) in somatic as well as germ cells of Drosophila melanogaster. For cytotoxicity, the productivity and some morphometric parameters were measured and genotoxicity was assessed by means of the somatic mutation and recombination test assay in the wing...
2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28629181/knockout-of-murine-mamld1-impairs-testicular-growth-and-daily-sperm-production-but-permits-normal-postnatal-androgen-production-and-fertility
#13
Mami Miyado, Kaoru Yoshida, Kenji Miyado, Momori Katsumi, Kazuki Saito, Shigeru Nakamura, Tsutomu Ogata, Maki Fukami
MAMLD1 has been implicated in testicular function in both human and mouse fetuses. Although three patients with MAMLD1 mutations were reported to have hypergonadotropic hypogonadism in their teens, the functional significance of MAMLD1 in the postnatal testis remains unclear. Here, we analyzed the phenotype of Mamld1 knockout (KO) male mice at reproductive ages. The reproductive organs of KO male mice were morphologically unremarkable, except for relatively small testes. Seminiferous tubule size and number of proliferating spermatogonia/spermatocytes were reduced in the KO testis...
June 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28624768/a-revised-asingle-model-to-explain-stem-cell-dynamics-in-the-mouse-male-germline
#14
REVIEW
Tessa Lord, Jon M Oatley
Spermatogonial stem cells (SSCs) and progenitor spermatogonia encompass the undifferentiated spermatogonial pool in mammalian testes. In rodents, this population is comprised of Asingle, Apaired and chains of 4-16 Aaligned spermatogonia. Although traditional models propose that the entire Asingle pool represents SSCs, and formation of an Apaired syncytium symbolizes irreversible entry to a progenitor state destined for differentiation; recent models have emerged that suggest that the Asingle pool is heterogeneous, and Apaired/Aaligned can fragment to produce new SSCs...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28615530/in-vivo-autophagy-and-biogenesis-of-autophagosomes-within-male-haploid-cells-during-spermiogenesis
#15
Ping Yang, Nisar Ahmed, Lingling Wang, Hong Chen, Yasir Waqas, Tengfei Liu, Abdul Haseeb, Nasrullah Bangulzai, Yufei Huang, Qiusheng Chen
Autophagy is a unique catabolic pathway that is linked to several physiological processes. However, its role in the process of spermiogenesis is largely unknown. The aim of the current study was to determine the in vivo role of autophagy and the origin of autophagosome membrane biogenesis within male haploid cells. Our immunohistochemistry results demonstrated that LC3 and ATG7 localization were increased dramatically in round to elongated spermatids (haploid cells) towards the lumen of seminiferous tubules, however, poorly expressed in the early stages of germ cells near the basal membrane...
May 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28611311/genome-wide-5-hydroxymethylcytosine-patterns-in-human-spermatogenesis-are-associated-with-semen-quality
#16
Olga A Efimova, Anna A Pendina, Andrei V Tikhonov, Sergey E Parfenyev, Irina D Mekina, Evgeniia M Komarova, Mariia A Mazilina, Eugene V Daev, Olga G Chiryaeva, Ilona A Galembo, Mikhail I Krapivin, Oleg S Glotov, Irina S Stepanova, Svetlana A Shlykova, Igor Yu. Kogan, Alexander M Gzgzyan, Tatyana V Kuznetzova, Vladislav S Baranov
We performed immunofluorescent analysis of DNA hydroxymethylation and methylation in human testicular spermatogenic cells from azoospermic patients and ejaculated spermatozoa from sperm donors and patients from infertile couples. In contrast to methylation which was present throughout spermatogenesis, hydroxymethylation was either high or almost undetectable in both spermatogenic cells and ejaculated spermatozoa. On testicular cytogenetic preparations, 5-hydroxymethylcytosine was undetectable in mitotic and meiotic chromosomes, and was present exclusively in interphase spermatogonia Ad and in a minor spermatid population...
June 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28611113/active-paraoxonase-1-is-synthesised-throughout-the-internal-boar-genital-organs
#17
Isabel Barranco, Cristina Perez-Patiño, Asta Tvarijonaviciute, Inmaculada Parrilla, Alejandro Vicente-Carrillo, Manuel Alvarez-Rodriguez, Jose J Ceron, Emilio Martinez, Heriberto Rodriguez-Martinez, Jordi Roca
The paraoxonase type 1 (PON1) is an enzyme with antioxidant properties recently identified in the seminal plasma (SP) of several species, including the porcine. The aims of the present study were to (1) describe the inmunohistochemical localization of PON1 in the genital organs of fertile boars and (2) evaluate the relationship among PON1 activity and high-density lipoprotein cholesterol (HDL-C) concentration in fluids of the boar genital organs. Immunohistochemical analysis demonstrated that PON1 was present in testis (specifically in Leydig cells, blood vessels, spermatogonia and elongated spermatids), epididymis (specifically in the cytoplasm of the principal epithelial cells, luminal secretion and in the surrounding smooth muscle) and the lining epithelia of the accessory sexual glands (cytoplasmic location in the prostate and membranous in the seminal vesicle and bulbourethral glands)...
June 13, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28608322/long-evans-rat-spermatogonial-lines-are-effective-germline-vectors-for-transgenic-rat-production
#18
F Kent Hamra, Christopher T Richie, Brandon K Harvey
Long Evans rat strains are applied as research models in a broad spectrum of biomedical fields (>15,800 citations, NCBI PubMed). Here, we report an approach to genetically modify the Long Evans rat germline in donor spermatogonial stem cells. Long Evans rat spermatogonial lines were derived from freshly isolated laminin-binding spermatogonia. Laminin-binding spermatogonia were cultured over multiple passages on fibroblast feeder layers in serum-free culture medium containing GDNF and FGF2. Long Evans rat spermatogonial lines were genetically modified by transposon transduction to express a germline, tdTomato reporter gene...
August 2017: Transgenic Research
https://www.readbyqxmd.com/read/28600885/immunolocalization-of-tar-dna-binding-protein-of-43%C3%A2-kda-tdp-43-in-mouse-seminiferous-epithelium
#19
Hari Prasad Osuru, Patcharin Pramoonjago, Mayuresh M Abhyankar, Eric Swanson, LaToya Ann Roker, Helen Cathro, Prabhakara P Reddi
TAR DNA-binding protein of 43 kDa (TDP-43) is an evolutionarily conserved, ubiquitously expressed, multi-functional DNA/RNA-binding protein with roles in gene transcription, mRNA splicing, stability, transport, micro RNA biogenesis, and suppression of transposons. Aberrant expression of TDP-43 in testis and sperm was recently shown to be associated with male infertility, which highlights the need to understand better the expression of TDP-43 in the testis. We previously cloned TDP-43 from a mouse testis cDNA library, and showed that it functions as a transcriptional repressor and regulates the precise spatiotemporal expression of the Acrv1 gene, which encodes the acrosomal protein SP-10, during spermatogenesis...
June 10, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28585553/nedd4-controls-spermatogonial-stem-cell-homeostasis-and-stress-response-by-regulating-messenger-ribonucleoprotein-complexes
#20
Zhi Zhou, Hiroshi Kawabe, Atsushi Suzuki, Kaori Shinmyozu, Yumiko Saga
P bodies (PBs) and stress granules (SGs) are conserved cytoplasmic aggregates of cellular messenger ribonucleoprotein complexes (mRNPs) that are implicated in mRNA metabolism and play crucial roles in adult stem cell homeostasis and stress responses. However, the mechanisms underlying the dynamics of mRNP granules are poorly understood. Here, we report NEDD4, an E3 ubiquitin ligase, as a key regulator of mRNP dynamics that controls the size of the spermatogonial progenitor cell (SPC) pool. We find that NEDD4 targets an RNA-binding protein, NANOS2, in spermatogonia to destabilize it, leading to cell differentiation...
June 6, 2017: Nature Communications
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