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https://www.readbyqxmd.com/read/29456864/curative-gnrha-treatment-has-an-unexpected-repressive-effect-on-sertoli-cell-specific-genes
#1
Katharina Gegenschatz-Schmid, Gilvydas Verkauskas, Philippe Demougin, Vytautas Bilius, Darius Dasevicius, Michael B Stadler, Faruk Hadziselimovic
Background: Follicle stimulating hormone and testosterone stimulate Sertoli cells to support germ cell function and differentiation. During mini-puberty, when gonadotropin (GnRH) stimulates increases in plasma luteinizing hormone (LH) and testosterone levels, gonocytes are transformed into Ad spermatogonia. In cryptorchidism, impaired gonadotropin secretion during mini-puberty results in insufficient LH and testosterone secretion, impaired gonocyte transition to Ad spermatogonia, and perturbed Sertoli cell proliferation...
2018: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/29453757/localization-of-epithelial-sodium-channel-enac-and-cftr-in-the-germinal-epithelium-of-the-testis-sertoli-cells-and-spermatozoa
#2
Sachin Sharma, Aaron Hanukoglu, Israel Hanukoglu
Spermatogenesis starts within the seminiferous tubules of the testis by mitotic division of spermatogonia that produces spermatocytes. Meiotic division of these spermatocytes produces haploid spermatids that differentiate into spermatozoa. In this study, we examined the expression of ENaC and CFTR (a Cl - channel) in rat testicular sections using confocal microscopic immunofluorescence. The structural integrity of the seminiferous tubule sections was verified by precise phalloidin staining of the actin fibers located abundantly at both basal and adluminal tight junctions...
February 16, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29452353/a-diagnostic-germ-cell-score-for-immature-testicular-tissue-at-risk-of-germ-cell-loss
#3
L Heckmann, D Langenstroth-Röwer, T Pock, J Wistuba, J-B Stukenborg, M Zitzmann, S Kliesch, S Schlatt, N Neuhaus
STUDY QUESTION: Can a systematic scoring procedure provide crucial information on the status of highly heterogeneous immature human testicular tissues in the context of cryopreservation for fertility preservation? SUMMARY ANSWER: We developed a systematic histological score as a novel diagnostic tool which differentiates the patient cohort according to the status of germ cell differentiation and number of spermatogonia (normal, diminished and absent), and which could be relevant in the fertility clinic...
February 14, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29443055/a-seminiferous-tubule-squash-technique-for-the-cytological-analysis-of-spermatogenesis-using-the-mouse-model
#4
Stephen R Wellard, Jessica Hopkins, Philip W Jordan
Meiotic progression in males is a process that requires the concerted action of a number of highly regulated cellular events. Errors occurring during meiosis can lead to infertility, pregnancy loss or genetic defects. Commencing at the onset of puberty and continuing throughout adulthood, continuous semi-synchronous waves of spermatocytes undergo spermatogenesis and ultimately form haploid sperm. The first wave of mouse spermatocytes undergoing meiotic initiation appear at day 10 post-partum (10 dpp) and are released into the lumen of seminiferous tubules as mature sperm at 35 dpp...
February 6, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29432730/prolyl-isomerase-pin1-is-required-sperm-production-by-promoting-mitosis-progression-of-spermatogonial-stem-cells
#5
Atsuko Kurita-Suzuki, Yuki Kamo, Chiyoko Uchida, Kentaro Tanemura, Kenshiro Hara, Takafumi Uchida
A prolyl isomerase Pin1 deficient (Pin1-/-) male mice had severe testicular atrophy. We investigated the function of Pin1 in spermatogenesis by analyzing the Pin1-/- mice at reproductive age. Pin1-/- mice had lessαPLZF positive spermatogonia (undifferentiated spermatogonia) than wild type (WT). Nevertheless, the Pin1-/- testis contained approximately the same number of GFRα1 positive spermatogonia (SSCs in steady state) as the WT testis. Furthermore, degeneration of the spermatogenia appeared in seminiferous tubules of 10 months old Pin1-/- mouse testis, and abnormal shape GFRα1 positive spermatogonia were observed...
February 9, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29415862/beyond-stem-cells-commitment-of-progenitor-cells-to-meiosis
#6
Michael D Griswold, Cathryn Hogarth
The first step in established spermatogenesis is the production of progenitor cells by the stem cell population. The progenitor cells (undifferentiated A spermatogonia) expand in number via the formation of syncytial chains by mitosis. The mechanism by which these progenitor cells commit to meiosis and spermatogenesis is tightly controlled and results in complex morphological organization all of which is designed to efficiently achieve large numbers of spermatozoa. The major extrinsic factor that triggers the commitment to meiosis and establishes the structural complexity is retinoic acid (RA)...
January 31, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29414992/can-we-induce-spermatogenesis-in-the-domestic-cat-using-an-in-vitro-tissue-culture-approach
#7
Andreia F Silva, Sara Escada-Rebelo, Sandra Amaral, Renata S Tavares, Stefan Schlatt, João Ramalho-Santos, Paula C Mota
The reduced number of animals in most wild felid populations implies a loss of genetic diversity. The death of juveniles, prior to the production of mature sperm, represents a loss of potential genetic contribution to future populations. Since 2011 mouse testicular organ culture has introduced an alternative mechanism to produce sperm in vitro from immature tissue. However, extension of this technology to other species has remained limited. We have used the domestic cat (Felis catus) as a model for wild felids to investigate spermatogenesis initiation and regulation, with the mouse serving as a control species...
2018: PloS One
https://www.readbyqxmd.com/read/29410003/pig-kdm5b-mrna-expression-profiles-of-different-tissues-and-testicular-cells-and-association-analyses-with-testicular-morphology-traits
#8
Yang Cui, Yanghai Zhang, Zhenyu Wei, Jiayang Gao, Ting Yu, Rui Chen, Xiaoyan Lv, Chuanying Pan
Lysine specific demethylase 5B gene (KDM5B, also known as JARID1B or PLU-1), encoding an enzyme of the lysine-specific histone demethylase family, has been reported to regulate androgen receptor transcriptional activity and male reproduction. To fully study the expression characteristics and genetic effects of pig KDM5B gene, the objective of this study was to investigate the mRNA expression profiles of KDM5B among different tissues and testicular cells (spermatogonia stem cells, SSCs; sertoli cells, SCs; leydig cells, LCs), as well as to explore the insertion/deletion (indel) variations of this gene...
January 30, 2018: Gene
https://www.readbyqxmd.com/read/29407897/species-specific-expression-of-phosphoglycerate-kinase-2-pgk2-in-the-developing-porcine-testis
#9
Hyun-Jung Park, Won-Young Lee, Chankyu Park, Kwon-Ho Hong, Jin-Hoi Kim, Hyuk Song
Whereas stage-specific markers for spermatogonial cells have been well investigated in mouse, the specific markers of germ cells in the testis of domestic animals have not been well defined. Phosphoglycerate kinase (PGK), an enzyme that converts 1,3-bisphosphoglycerate and adenosine diphosphate to 3-phosphoglycerate and adenosine triphosphate, has two isozymes: PGK1 and PGK2. In mouse, PGK1 exists only during the early stages of spermatogenesis, and PGK2 is then expressed during the pachytene spermatocyte stage...
February 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/29406096/exposure-to-difenoconazole-inhibits-reproductive-ability-in-male-marine-medaka-oryzias-melastigma
#10
Xiaocui Dong, Lemeng Zhang, Meng Chen, Zhibin Yang, Zhenghong Zuo, Chonggang Wang
Difenoconazole (DFZ) is a triazole fungicide which has been detected in the aquatic environment, including estuaries and embayments. However, few studies addressing the reproductive toxicity and transgenerational effects of DFZ on marine fishes are available. The present study was conducted to investigate the effects of DFZ on male marine medaka (Oryzias melastigma). After exposure of the embryo to 1, 10, 100 and 1000ng/L DFZ for 180days, the gonadosomatic index was significantly decreased in the 1000ng/L treatment...
January 2018: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/29398482/testicular-architecture-is-critical-for-mediation-of-retinoic-acid-responsiveness-by-undifferentiated-spermatogonial-subtypes-in-the-mouse
#11
Tessa Lord, Melissa J Oatley, Jon M Oatley
Spermatogenesis requires retinoic acid (RA) induction of the undifferentiated to differentiating transition in transit amplifying (TA) progenitor spermatogonia, whereas continuity of the spermatogenic lineage relies on the RA response being suppressed in spermatogonial stem cells (SSCs). Here, we discovered that, in mouse testes, both spermatogonial populations possess intrinsic RA-response machinery and exhibit hallmarks of the differentiating transition following direct exposure to RA, including loss of SSC regenerative capacity...
January 29, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29393557/talen-mediated-gene-targeting-in-porcine-spermatogonia
#12
Lin Tang, Alla Bondareva, Raquel González, Jose Rafael Rodriguez-Sosa, Daniel F Carlson, Dennis Webster, Scott Fahrenkrug, Ina Dobrinski
Spermatogonia represent a diploid germ cell population that includes spermatogonial stem cells. In this report, we describe new methods for isolation of highly enriched porcine spermatogonia based on light scatter properties, and for targeted mutagenesis in porcine spermatogonia using nucleofection and TALENs. We optimized a nucleofection protocol to deliver TALENs specifically targeting the DMD locus in porcine spermatogonia. We also validated specific sorting of porcine spermatogonia based on light scatter properties...
February 2, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29387828/effects-of-administration-of-co-trimoxazole-and-folic-acid-on-sperm-quality-and-histological-changes-of-testes-in-male-rats
#13
Ehsan Salarkia, Gholamreza Sepehri, Parvin Torabzadeh, Jalil Abshenas, Arezoo Saberi
Background: Male infertility has been reported following long-term sulfasalazine, however, the precise effects of co-trimoxazole on sperm quality is controversial. Objective: In this study, we evaluated the effects of co-trimoxazole and its co-administration with folic acid on sperm quality and histological changes of testes in male rats. Materials and Methods: In this experimental study, 136 male Wistar rats were divided into 9 groups: I (control), II (vehicle) received saline, III: received folic acid (1 mg/kg /daily i...
October 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29386567/mgat1-and-complex-n-glycans-regulate-erk-signaling-during-spermatogenesis
#14
Barnali Biswas, Frank Batista, Subha Sundaram, Pamela Stanley
Mechanisms that regulate spermatogenesis in mice are important to define as they often apply to fertility in man. We previously showed that conditional deletion of the mouse Mgat1 gene (Mgat1 cKO) in spermatogonia causes a germ-cell autonomous defect leading to infertility. MGAT1 is the N-acetylglucosaminyltransferase (GlcNAcT-I) that initiates the synthesis of complex N-glycans. Mechanistic bases of MGAT1 loss were investigated in germ cells from 22- and 23-day males, before any changes in germ cell morphology were apparent...
January 31, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29385162/the-balance-between-apoptosis-and-autophagy-regulates-testis-regression-and-recrudescence-in-the-seasonal-breeding-south-american-plains-vizcacha-lagostomus-maximus
#15
Candela R González, María L Muscarsel Isla, Alfredo D Vitullo
Mammalian testis undergoes deep changes in their architecture and function during photoregression conditions in seasonal breeders. Particularly, the testicular mechanisms that regulate the transition between the active (functional) and inactive (regression) stage vary between species. The aim of the present study was to analyze the incidence of proliferation, apoptosis and autophagy in the testicular seminiferous ephitelium of a seasonal breeder, Lagostomus maximus, during the annual reproductive cycle. We observed that proliferating spermatogonia increased from the active testis until reaching the maximum peak in the activating testis...
2018: PloS One
https://www.readbyqxmd.com/read/29384219/pirna-19128-regulates-spermatogenesis-by-silencing-of-kit-in-chicken
#16
Qixin Guo, Lu Xu, Yulin Bi, Lingling Qiu, Yin Chen, Lingling Kong, Rui Pan, Guobin Chang
Spermatogenesis is a complex process. Some studies have shown that Piwi-interacting RNAs (piRNAs) play an important role in spermatogenesis. To verify the evaluate between piRNAs and PIWI proteins in chicken and its possible role in spermatogenesis and reproductive stem cell proliferation and differentiation, we performed immunoprecipitation and deep sequencing analyses and determined the expression profiles of small RNAs in primordial germ cells (PGCs), spermatogonial stem cells (SSCs), spermatogonia (Sa) cells and spermatozoa...
January 31, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29383560/expression-profiles-of-sox-transcription-factors-within-the-postnatal-rodent-testes
#17
Pauline Roumaud, Josée Haché, Luc J Martin
SRY-related box (Sox) transcription factors are conserved among vertebrate species. These proteins regulate multiple processes including sex determination and testis differentiation of the male embryo. Members of the Sox family have been identified in pre- and postnatal testis and are known to play an important role in sex determination (Sry, Sox9), male gonadal development, and fertility (Sox4, Sox8, Sox30). However, their expression profiles per cell types remain elusive. The objectives of this research were to characterize the expression profiles of Sox family members within adult testes using publically available datasets and to determine whether these findings are consistent with literature as well as immunofluorescence and in situ hybridization results...
January 30, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29381885/differential-expression-profiles-of-conserved-snail-transcription-factors-in-the-mouse-testis
#18
D J Micati, G R Hime, E A McLaughlin, H E Abud, K L Loveland
Snail transcription factors are key regulators of cellular transitions during embryonic development and tumorigenesis. The closely related SNAI1 and SNAI2 proteins induce epithelial-mesenchymal transitions (EMTs), acting predominantly as transcriptional repressors, while the functions of SNAI3 are unknown. An initial examination of Snai2-deficient mice provided evidence of deficient spermatogenesis. To address the hypothesis that Snail proteins are important for male fertility, this study provides the first comprehensive cellular expression profiles of all three mammalian Snail genes in the post-natal mouse testis...
January 30, 2018: Andrology
https://www.readbyqxmd.com/read/29372036/histomorphometric-evaluation-of-mice-testicular-tissue-following-short-and-long-term-effects-of-lipopolysaccharide-induced-endotoxemia
#19
Oveis Jafari, Homayoon Babaei, Reza Kheirandish, Amir-Saied Samimi, Ali Zahmatkesh
Objectives: Lipopolysaccharide (LPS)-induced endotoxemia is known to cause male infertility. This study was designed to explore the effects of bacterial LPS on histomorphometric changes of mice testicular tissues. Materials and Methods: In experiment 1, a pilot dose responsive study was performed with mice that were divided into five groups, receiving 36000, 18000, 9000, and 6750 µg/kg body weight (B.W) of LPS or only saline (control). White blood cells (WBC) were observed for 3 days after LPS inoculation...
January 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29362488/transcriptional-regulation-of-p63-on-the-apoptosis-of-male-germ-cells-and-three-stages-of-spermatogenesis-in-mice
#20
Hong Wang, Qingqing Yuan, Minghui Niu, Wenhui Zhang, Liping Wen, Hongyong Fu, Fan Zhou, Zuping He
Infertility affects 10-15% of couples worldwide, and male factors account for 50%. Spermatogenesis is precisely regulated by genetic factors, and the mutations of genes result in abnormal spermatogenesis and eventual male infertility. The aim of this study was to explore the role and transcriptional regulation of P63 in the apoptosis and mouse spermatogenesis. P63 protein was decreased in male germ cells of P63(+/-) mice compared with wild-type mice. There was no obvious difference in testis weight, sperm motility, and fecundity between P63(+/-) and wild-type mice...
January 23, 2018: Cell Death & Disease
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