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https://www.readbyqxmd.com/read/28214901/protective-effects-of-fluvastatin-on-reproductive-function-in-obese-male-rats-induced-by-high-fat-diet-through-enhanced-signaling-of-mtor
#1
Xiangrong Cui, Chunlan Long, Jing Zhu, Jie Tian
BACKGROUND: Statins can reduce reproductive damage induced by obesity or high-fat diet (HFD), but the specific regulatory mechanisms are largely unknown. Since mTOR/p70s6k sinaling promotes spermatogonia proliferation and spermatogenesis, we hypothesized that this pathway will be involved in the protective effects of statin in HFD-induced reproductive dysfunction. METHODS: Male Sprague Dawley rats (3 weeks old) were randomly divided into a control group (standard diet), HFD group, and a fluvastatin group (HFD + fluvastatin at 6mg/kg, once daily by oral gavage)...
February 3, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28211534/in-vitro-differentiation-of-fertile-sperm-from-cryopreserved-spermatogonia-of-the-endangered-endemic-cyprinid-honmoroko-gnathopogon-caerulescens
#2
Shogo Higaki, Manami Shimada, Kazuaki Kawamoto, Takaaki Todo, Toshihiro Kawasaki, Ikuo Tooyama, Yasuhiro Fujioka, Noriyoshi Sakai, Tatsuyuki Takada
Many endemic fish species are threatened with extinction. Conservation strategies and the restoration of endemic fish after extinction must therefore be investigated. Although sperm cryopreservation is indispensable for the conservation of endangered fishes, the limited number of mature fish and limited availability (volume and period) of sperm from small endemic fish hinders the optimization and practical use of this material. In this report, we demonstrate the in vitro differentiation of fertile sperm from cryopreserved spermatogonia of juveniles of the endangered small cyprinid honmoroko (Gnathopogon caerulescens), which is endemic to Lake Biwa in Japan...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205273/in-vitro-responses-to-known-in-vivo-genotoxic-agents-in-mouse-germ-cells
#3
Khaled Habas, Martin H Brinkworth, Diana Anderson
Genotoxic compounds have induced DNA damage in male germ cells and have been associated with adverse clinical outcomes including enhanced risks for maternal, paternal and offspring health. DNA strand breaks represent a great threat to the genomic integrity of germ cells. Such integrity is essential to maintain spermatogenesis and prevent reproduction failure. The Comet assay results revealed that the incubation of isolated germ cells with n-ethyl-n-nitrosourea (ENU), 6-mercaptopurine (6-MP) and methyl methanesulphonate (MMS) led to increase in length of Olive tail moment and % tail DNA when compared with the untreated control cells and these effects were concentration-dependent...
February 16, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/28196764/nuclear-and-membrane-progestin-receptors-in-the-european-eel-characterization-and-expression-in-vivo-through-spermatogenesis
#4
Marina Morini, David S Peñaranda, María C Vílchez, Rasoul Nourizadeh-Lillabadi, Anne-Gaëlle Lafont, Sylvie Dufour, Juan F Asturiano, Finn-Arne Weltzien, Luz Pérez
Characterization of all the progestin receptor genes (PRs) found in the European eel has been performed. There were five membrane PRs (mPRs): mPRα (alpha), mPRAL1 (alpha-like1), mPRAL2 (alpha-like2), mPRγ (gamma), mPRδ (delta) and two nuclear PRs (nPRs or PGRs): pgr1 and pgr2. In silico studies showed that the C and E(F) domains of Pgr are well conserved among vertebrates whereas the A/B domain is not. Phylogeny and synteny analyses suggest that eel duplicated pgr (pgr1 and pgr2) originated from the teleost-specific third whole genome duplication (3R)...
February 11, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28196692/shisa6-confers-resistance-to-differentiation-promoting-wnt-%C3%AE-catenin-signaling-in-mouse-spermatogenic-stem-cells
#5
Moe Tokue, Kanako Ikami, Seiya Mizuno, Chiyo Takagi, Asuka Miyagi, Ritsuko Takada, Chiyo Noda, Yu Kitadate, Kenshiro Hara, Hiroko Mizuguchi, Takuya Sato, Makoto Mark Taketo, Fumihiro Sugiyama, Takehiko Ogawa, Satoru Kobayashi, Naoto Ueno, Satoru Takahashi, Shinji Takada, Shosei Yoshida
In the seminiferous tubules of mouse testes, a population of glial cell line-derived neurotrophic factor family receptor alpha 1 (GFRα1)-positive spermatogonia harbors the stem cell functionality and supports continual spermatogenesis, likely independent of asymmetric division or definitive niche control. Here, we show that activation of Wnt/β-catenin signaling promotes spermatogonial differentiation and reduces the GFRα1(+) cell pool. We further discovered that SHISA6 is a cell-autonomous Wnt inhibitor that is expressed in a restricted subset of GFRα1(+) cells and confers resistance to the Wnt/β-catenin signaling...
February 7, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28191733/a-novel-dynamic-expression-of-vasa-in-male-germ-cells-during-spermatogenesis-in-the-chinese-soft-shell-turtle-pelidiscus-sinensis
#6
Wei Li, Piaoyi Zhang, Xuling Wu, Xinping Zhu, Hongyan Xu
vasa gene encodes a highly conserved DEAD-box RNA helicase, required for germ cell development across animal kingdom. Vasa mutations cause male infertility in mammals. It has been widely used as a biomarker for studying animal fertility or manipulating germ cells in organisms. However, in reptilians, the functions of vasa gene involved in germ cell differentiation are largely unclear; this hampers the development of biological techniques and the improvement of the productivity in these species. Here a vasa cDNA was isolated in Chinese soft-shell turtle and it predicts a protein of 691 amino acid residues, which is 72%, 69%, 58%, 59%, and 54-56% identical to its homolog from mouse, platypus, frog, chicken, and fish, respectively, and named as PsVasa...
February 13, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28190795/progress-in-understanding-the-molecular-functions-of-ddx3y-dby-in-male-germ-cell-development-and-maintenance
#7
Alexei A Kotov, Oxana M Olenkina, Baira K Godneeva, Vladimir E Adashev, Ludmila V Olenina
Human DDX3 paralogs are housed on the X chromosome (DDX3X) as well as in the non- recombining region Yq11 of the Y-chromosome (DDX3Y or DBY). A gene encoding RNA helicase DDX3Y is located in the AZoospermia Factor a (AZFa) region of the Y-chromosome and expressed only in male germ cells. Deletions encompassing the DDX3Y gene lead to azoospermia and cause Sertoli Cell-Only Syndrome (SCOS) in humans. SCOS is characterized by a complete germ cell lack with preservation of somatic Sertoli cells. This review summarizes current advances in the study of DDX3Y functions in maintenance and development of early male germ cells...
February 12, 2017: Bioscience Trends
https://www.readbyqxmd.com/read/28181310/localization-of-orexin-b-and-receptor-2-for-orexins-in-testicular-cytotypes-of-the-camelid-alpaca-vicugna-pacos
#8
G Liguori, C Squillacioti, L Assisi, N Mirabella, E Langella, A Costagliola, A Vittoria
The orexins A (OxA) and B (OxB) are two hypothalamic peptides involved in many physiological functions of the mammalian body. They act through the binding of two G-coupled receptors named receptor 1 (OX1 ) and receptor 2 (OX2 ) for orexins. The first receptor is specific for OxA, while the second binds both the substances with equal affinity. The orexins and the relative receptors have been traced by means of different techniques also at the periphery of the body and particularly in the adrenals, and in gastrointestinal and genital organs...
February 8, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28160442/xenografting-of-isolated-equine-equus-caballus-testis-cells-results-in-de-novo-morphogenesis-of-seminiferous-tubules-but-not-spermatogenesis
#9
W Zeng, W Alpaugh, D Stefanovski, K Schlingmann, I Dobrinski, R M Turner
The study of spermatogenesis in the horse is challenging because of the absence of an in vitro system that is capable of reproducing efficient spermatogenesis and because of the difficulties and costs associated with performing well-controlled studies in vivo. In an attempt to develop novel methods for the study of equine spermatogenesis, we tested whether cells from enzymatically digested pre-pubertal equine testicular tissue were capable of de novo tissue formation and spermatogenesis following xenografting under the back skin of immunocompromised mice...
February 3, 2017: Andrology
https://www.readbyqxmd.com/read/28144634/gonadogenesis-and-sex-differentiation-in-the-korean-rose-bitterling-rhodeus-uyekii
#10
In Bon Goo, Jung Eun Kim, Myung Hun Kim, Hye-Sung Choi, Hee Jeong Kong, Jeong Ho Lee, In-Seok Park
This report describes the sex differentiation of the Korean rose bitterling, Rhodeus uyekii, from hatching to 170 days post-hatch (DPH) in relation to total length (TL), body weight (BW), and integral water temperature (IWT). The growth curve of TL from just hatching to 83 DPH was 5.144e(0.045t) (R² = 0.961; t, time), and that of BW was 2.398e(0.086t) (R² = 0.725). Primordial germ cells (PGCs) were observed at 17 DPH (7.9 mm TL, 3.74 mg BW, 374°C IWT), and thereafter began to protrude into the peritoneal cavity...
December 2016: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28142151/novel-amh-and-amhr2-mutations-in-two-egyptian-families-with-persistent-m%C3%A3-llerian-duct-syndrome
#11
Inas Mazen, Mona El-Gammal, Ken McElreavey, Aya Elaidy, Mohamed S Abdel-Hamid
Anti-müllerian hormone (AMH) is produced by Sertoli cells and signals through 2 transmembrane receptors (AMHR), specific types I and II, leading to regression of müllerian ducts during fetal male sex differentiation. Mutations in AMH and AMHR2 lead to the persistence of müllerian ducts in males which is transmitted in a recessive pattern. Here, we report 2 Egyptian DSD (disorder of sex development) patients reared as males who presented with bilateral cryptorchidism and otherwise normal male external genitalia and who both had a 46,XY karyotype...
February 1, 2017: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
https://www.readbyqxmd.com/read/28130393/irradiation-affects-germ-and-somatic-cells-in-prepubertal-monkey-testis-xenografts
#12
I Tröndle, B Westernströer, J Wistuba, N Terwort, S Schlatt, N Neuhaus
STUDY QUESTION: Does irradiation evoke adverse effects in germ and somatic cells in testis xenografts from prepubertal monkeys? SUMMARY ANSWER: In addition to the expected depletion of germ cells, a dose-dependent effect of irradiation was observed at the mRNA and protein level in Sertoli and peritubular myoid cells. WHAT IS KNOWN ALREADY: Testicular irradiation studies in monkeys have focused on the dose-dependent effects on germ cells. Previous studies using intact animals or xenografts reported that germ cells are highly sensitive to irradiation...
January 27, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28128212/bcas2-is-involved-in-alternative-mrna-splicing-in-spermatogonia-and-the-transition-to-meiosis
#13
Wenbo Liu, Fengchao Wang, Qianhua Xu, Junchao Shi, Xiaoxin Zhang, Xukun Lu, Zhen-Ao Zhao, Zheng Gao, Huaixiao Ma, Enkui Duan, Fei Gao, Shaorong Gao, Zhaohong Yi, Lei Li
Breast cancer amplified sequence 2 (BCAS2) is involved in multiple biological processes, including pre-mRNA splicing. However, the physiological roles of BCAS2 are still largely unclear. Here we report that BCAS2 is specifically enriched in spermatogonia of mouse testes. Conditional disruption of Bcas2 in male germ cells impairs spermatogenesis and leads to male mouse infertility. Although the spermatogonia appear grossly normal, spermatocytes in meiosis prophase I and meiosis events (recombination and synapsis) are rarely observed in the BCAS2-depleted testis...
January 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/28109954/a-role-of-kit-receptor-signaling-for-proliferation-and-differentiation-of-rat-stem-leydig-cells-in%C3%A2-vitro
#14
Shiwen Liu, Xiaomin Chen, Yiyan Wang, Linxi Li, Guimin Wang, Xiaoheng Li, Haolin Chen, Jingjing Guo, Han Lin, Qing-Quan Lian, Ren-Shan Ge
In the testis, KIT ligand (KITL, also called stem cell factor) is expressed by Sertoli cells and its receptor (c-kit, KIT) is expressed by spermatogonia and Leydig cells. Although KITL-KIT signaling is critical for the spermatogenesis, its roles in Leydig cell development during puberty are not clear. In the present study, we investigated effects of KITL on stem Leydig cell proliferation and differentiation. Using an in vitro culture system of seminiferous tubules from Leydig cell-depleted testis, we found that KITL increased the proliferation activity of putative stem Leydig cells at higher concentration (10 and 100 ng/ml)...
January 18, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28109155/-preparation-and-application-of-rabbit-anti-mouse-setd8-polyclonal-antibody
#15
Haitao Ma, Jiaqian Guo, Jing Xia, Changmin Niu, Xueyi Shen, Hongya Sun, Ying Zheng
Objective To purify the recombinant Setd8 protein and prepare rabbit anti-mouse Setd8 polyclonal antibody. Methods The recombinant plasmid pET-30a-Setd8 was constructed by double enzyme digestion and linkage, and then transformed into E.coli BL21. The expression of the target protein was induced by IPTG and the expression product was purified by Ni-NTA affinity chromatograph. The purified protein was used to immunize New Zealand white rabbits to produce polyclonal antibody. The titer and specificity of the antibody were identified by ELISA, Western blotting and immunohistochemistry...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109031/undifferentiated-embryonic-cell-transcription-factor-1-utf1-and-deleted-in-azoospermia-like-dazl-expression-in-the-testes-of-donkeys
#16
Y S Lee, H J Jung, M J Yoon
Putative markers for each specific germ cell stage can be a useful tool to study the fate and functions of these cells. Undifferentiated embryonic cell transcription factor 1 (UTF1) is a putative marker for undifferentiated spermatogonia in humans, rats and horses. The deleted in azoospermia-like (DAZL) protein is also expressed by differentiated spermatogonia and primary spermatocytes in several species. However, whether the expression patterns of these molecular markers are identical and applicable to donkeys remains to be elucidated...
January 20, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28104696/systematic-characterization-of-human-testis-specific-actin-capping-protein-%C3%AE-3-as-a-possible-biomarker-for-male-infertility
#17
T Soda, Y Miyagawa, N Ueda, K Takezawa, H Okuda, S Fukuhara, K Fujita, H Kiuchi, M Uemura, Y Okamoto, A Tsujimura, H Tanaka, N Nonomura
STUDY QUESTION: Is actin capping protein (CP) β3 involved in human spermatogenesis and male infertility? SUMMARY ANSWER: Human CPβ3 (hCPβ3) is expressed in testis, changes its localization dynamically during spermatogenesis, and has some association with male infertility. WHAT IS KNOWN ALREADY: The testis-specific α subunit of CP (CPα3) was previously identified in human, and mutations in the cpα3 gene in mouse were shown to induce malformation of the sperm head and male infertility...
January 18, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28101891/different-expression-of-leptin-and-igf1-in-the-adult-and-prepubertal-testis-in-dogs
#18
L Müller, M P Kowalewski, I M Reichler, E Kollár, O Balogh
Leptin (Lep) and insulin-like growth factor 1 (IGF1) are implicated in the regulation of testicular function, but in dogs, our knowledge is limited to the possible role of the IGF1 system in testicular tumours. In this study, we aimed to describe and compare gene expression and protein localization of Lep, IGF1 and their receptors (LepR and IGF1R, respectively) in the testis of healthy adult and prepubertal dogs. Testes were collected from sexually healthy mature (n = 7) and from 8-week-old dogs (n = 7)...
January 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28099840/testis-specific-histone-variant-h3t-gene-is-essential-for-entry-into-spermatogenesis
#19
Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata
Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes...
January 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/28095297/dual-estrogenic-regulation-of-the-nuclear-progestin-receptor-and-spermatogonial-renewal-during-gilthead-seabream-sparus-aurata-spermatogenesis
#20
François Chauvigné, Janmejay Parhi, Judith Ollé, Joan Cerdà
Studies in teleosts suggest that progestins have crucial functions during early spermatogenesis. However, the role of the different progestin receptors in these mechanisms is poorly understood. In this work, we investigated the expression pattern and hormonal regulation of the classical nuclear progestin receptor (Pgr) in the gilthead seabream at three different stages of spermatogenesis: the resting (postspawning) phase, onset of spermatogenesis, and spermiation. Immunolocalization experiments using a seabream specific Pgr antibody revealed that the receptor was expressed in Sertoli and Leydig cells, and also in a subset of spermatogonia type A, throughout spermatogenesis...
January 15, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
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