keyword
MENU ▼
Read by QxMD icon Read
search

Human testis

keyword
https://www.readbyqxmd.com/read/29332656/germ-cell-tumor-causing-pleural-effusion-a-diagnostic-dilemma
#1
Surabhi Jaggi, Reetu Kundu, Sanjeev Binji, Uma Handa, Varinder Saini
Straw colored pleural fluid with raised adenosine deaminase (ADA) levels in young healthy adults usually raises suspicion of tuberculosis, sometimes leading to laxity in carrying thorough physical examination and missing out some important clues with potential disastrous consequences. A 35-year-old male was diagnosed to have left pleural effusion and anti-tubercular treatment was started on the basis of straw colored, lymphocyte-predominant pleural fluid with significantly raised ADA levels. When there was no improvement after 1 month of treatment he was investigated further and found to have a mediastinal mass along with hydro-pneumothorax...
January 2018: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/29331980/dmc1-mutation-that-causes-human-non-obstructive-azoospermia-and-premature-ovarian-insufficiency-identified-by-whole-exome-sequencing
#2
Wen-Bin He, Chao-Feng Tu, Qiang Liu, Lan-Lan Meng, Shi-Min Yuan, Ai-Xiang Luo, Fu-Sheng He, Juan Shen, Wen Li, Juan Du, Chang-Gao Zhong, Guang-Xiu Lu, Ge Lin, Li-Qing Fan, Yue-Qiu Tan
BACKGROUND: The genetic causes of the majority of male and female infertility caused by human non-obstructive azoospermia (NOA) and premature ovarian insufficiency (POI) with meiotic arrest are unknown. OBJECTIVE: To identify the genetic cause of NOA and POI in two affected members from a consanguineous Chinese family. METHODS: We performed whole-exome sequencing of DNA from both affected patients. The identified candidate causative gene was further verified by Sanger sequencing for pedigree analysis in this family...
January 13, 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/29331483/gata4-is-a-transcriptional-regulator-of-r-spondin1-in-japanese-flounder-paralichthys-olivaceus
#3
Xiumei Liu, Zan Li, Bo Wang, He Zhu, Yuezhong Liu, Jie Qi, Quanqi Zhang
GATA4 is a well-known transcription factor of the GATA family implicated in regulation of sex determination and gonadal development in mammals. In this study, we cloned the full-length cDNA of Paralichthys olivaceus gata4 (Po-gata4). Phylogenetic, gene structure, and synteny analysis showed that Po-GATA4 is homologous to GATA4 of teleost and tetrapod. Po-gata4 transcripts were detected in Sertoli cells, spermatogonia, oogonia and oocytes, with higher transcript levels overall in the testis than the ovary. The promoter region of P...
January 10, 2018: Gene
https://www.readbyqxmd.com/read/29329550/systematic-quantitative-analysis-of-h2a-and-h2b-variants-by-targeted-proteomics
#4
Sara El Kennani, Annie Adrait, Olga Permiakova, Anne-Marie Hesse, Côme Ialy-Radio, Myriam Ferro, Virginie Brun, Julie Cocquet, Jérôme Govin, Delphine Pflieger
BACKGROUND: Histones organize DNA into chromatin through a variety of processes. Among them, a vast diversity of histone variants can be incorporated into chromatin and finely modulate its organization and functionality. Classically, the study of histone variants has largely relied on antibody-based assays. However, antibodies have a limited efficiency to discriminate between highly similar histone variants. RESULTS: In this study, we established a mass spectrometry-based analysis to address this challenge...
January 12, 2018: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/29311296/ibuprofen-alters-human-testicular-physiology-to-produce-a-state-of-compensated-hypogonadism
#5
David Møbjerg Kristensen, Christèle Desdoits-Lethimonier, Abigail L Mackey, Marlene Danner Dalgaard, Federico De Masi, Cecilie Hurup Munkbøl, Bjarne Styrishave, Jean-Philippe Antignac, Bruno Le Bizec, Christian Platel, Anders Hay-Schmidt, Tina Kold Jensen, Laurianne Lesné, Séverine Mazaud-Guittot, Karsten Kristiansen, Søren Brunak, Michael Kjaer, Anders Juul, Bernard Jégou
Concern has been raised over increased male reproductive disorders in the Western world, and the disruption of male endocrinology has been suggested to play a central role. Several studies have shown that mild analgesics exposure during fetal life is associated with antiandrogenic effects and congenital malformations, but the effects on the adult man remain largely unknown. Through a clinical trial with young men exposed to ibuprofen, we show that the analgesic resulted in the clinical condition named "compensated hypogonadism," a condition prevalent among elderly men and associated with reproductive and physical disorders...
January 8, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29311018/melanoma-associated-antigen-a11-reduces-erlotinib-and-afatinib-efficacy-in-head-and-neck-cancer
#6
Stefan Hartmann, Leonie Zwick, Katja Maurus, Andreas R Fuchs, Roman C Brands, Axel Seher, Alexander C Kübler, Urs D A Müller-Richter
Melanoma-associated antigen A (MAGE-A) proteins are members of the cancer/testis antigens (CTA), and the expression of these proteins is almost exclusively limited to malignant cells, making them an attractive treatment target. MAGE-A expression is correlated with poor overall survival in several cancers, including head and neck squamous cell carcinoma (HNSCC). Among others, MAGE-A11 was found to be associated with resistance to different antineoplastic and targeted compounds, such as epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs)...
December 24, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29309804/experimental-models-of-testicular-development-and-function-using-human-tissue-and-cells
#7
REVIEW
Melissa Tharmalingam, Anne Jorgensen, Rod T Mitchell
The mammalian testis has two main roles, production of gametes for reproduction and synthesis of steroid- and peptide hormones for masculinization. These processes are tightly regulated and involve complex interactions between a number of germ and somatic cell-types that comprise a unique microenvironment known as the germ stem cell niche. In humans, failure of normal testicular development or function is associated with susceptibility to a variety of male reproductive disorders including disorders of sex development, infertility and testicular cancer...
January 5, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29304256/dynamics-ultrastructure-and-gene-expression-of-human-in-vitro-organized-testis-cells-from-testicular-sperm-extraction-biopsies
#8
Kathrein von Kopylow, Wolfgang Schulze, Andrea Salzbrunn, Matthias Schaks, Elke Schäfer, Beate Roth, Stefan Schlatt, Andrej-Nikolai Spiess
STUDY QUESTION: Is it possible to induce in-vitro reorganization of primary human testis cells from testicular sperm extraction (TESE) biopsies, maintain their long-term cultivation in a 2D system and identify cellular compositions? SUMMARY ANSWER: In-vitro reorganization of primary human testis cells from TESE biopsies and their long-term cultivation on uncoated cell culture dishes is feasible and the cellular compositions can be uncovered through gene expression and microscopic analyses...
January 3, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29298896/mechanistic-insights-into-acephalic-spermatozoa-syndrome-associated-mutations-in-the-human-sun5-gene
#9
Yongliang Shang, Jie Yan, Wenhao Tang, Chao Liu, Sai Xiao, Yueshuai Guo, Li Yuan, Liang Chen, Hui Jiang, Xuejiang Guo, Jie Qiao, Wei Li
Acephalic spermatozoa syndrome has been reported for many decades; it is characterized by very few intact spermatozoa and tailless sperm heads in the semen and causes severe male infertility. The only gene whose mutations have been found to be associated with this syndrome encodes Sad1 and UNC84 domain-containing 5 (SUN5), a testis-specific nuclear envelope protein. The functional role of SUN5 has been well studied in mouse models, but the molecular basis for the pathogenic effects of mutations in the human SUN5 gene remain elusive...
January 3, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29298885/recombinant-chimpanzee-adenovirus-vaccine-adc7-m-e-protects-against-zika-virus-infection-and-testis-damage
#10
Kun Xu, Yufeng Song, Lianpan Dai, Yongli Zhang, Xuancheng Lu, Yijia Xie, Hangjie Zhang, Tao Cheng, Qihui Wang, Qingrui Huang, Yuhai Bi, William J Liu, Wenjun Liu, Xiangdong Li, Chuan Qin, Yi Shi, Jinghua Yan, Dongming Zhou, George F Gao
The recent outbreak of Zika virus (ZIKV) has emerged as a global health concern. ZIKV can persist in human semen and be transmitted by sexual contact, as well as by mosquitos as seen for classical arboviruses. We and others have previously demonstrated that ZIKV infection leads to testis damage and infertility in mouse models. So far, no prophylactics or therapeutics are available, therefore, vaccine development is urgently demanded. Recombinant chimpanzee adenovirus has been explored as the preferred vaccine vector for many pathogens due to the low pre-existing immunity against the vector among the human population...
January 3, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29288959/ivermectin-acute-administration-impaired-the-spermatogenesis-and-spermiogenesis-of-adult-rats
#11
Flora Cordeiro, Vagner Gonçalves, Natalia Moreira, João Iosif Slobodticov, Nathalia de Andrade Galvão, Helenice de Souza Spinosa, Leoni Villano Bonamin, Eduardo Fernandes Bondan, Claudia Helena Pastor Ciscato, Cláudia Maria Barbosa, Maria Martha Bernardi
Ivermectin (IVM) is an antiparasitic agent widely used in agricultural, domestic animals and in human clinical practice. In the present study, the temporal effects of therapeutic doses of IVM in the morphometric and histological assessment of testis were studied to verify if IVM acute administration impaired the spermatogenesis and spermiogenesis of adult rats, if these effects are reversible. The testosterone levels and the plasmatic IVM levels were assessed. The results show: 1) IVM acute exposure, mainly in the higher dose, reduced the testicular volume, the tubular diameter and the germinal epithelium height; 2) no interferences on Leydig cells frequency; 3) histological studies show that tubular sections containing several histological changes indicative of spermatogenesis interruption, such as disorganization of germinal epithelium, vacuolar degeneration of the germ cells and sloughing of cells into the tubular lumen; 4) no differences in testosterone levels; 5) The IVM plasmatic levels were significantly reduced at 72h after the 0...
December 18, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29259455/human-male-infertility-and-its-genetic-causes
#12
REVIEW
Toshinobu Miyamoto, Gaku Minase, Takeshi Shin, Hiroto Ueda, Hiroshi Okada, Kazuo Sengoku
Background: Infertility affects about 15% of couples who wish to have children and half of these cases are associated with male factors. Genetic causes of azoospermia include chromosomal abnormalities, Y chromosome microdeletions, and specific mutations/deletions of several Y chromosome genes. Many researchers have analyzed genes in the AZF region on the Y chromosome; however, in 2003 the SYCP3 gene on chromosome 12 (12q23) was identified as causing azoospermia by meiotic arrest through a point mutation...
April 2017: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29250030/cytokines-in-male-fertility-and-reproductive-pathologies-immunoregulation-and-beyond
#13
REVIEW
Kate L Loveland, Britta Klein, Dana Pueschl, Sivanjah Indumathy, Martin Bergmann, Bruce E Loveland, Mark P Hedger, Hans-Christian Schuppe
Germline development in vivo is dependent on the environment formed by somatic cells and the differentiation cues they provide; hence, the impact of local factors is highly relevant to the production of sperm. Knowledge of how somatic and germline cells interact is central to achieving biomedical goals relating to restoring, preserving or restricting fertility in humans. This review discusses the growing understanding of how cytokines contribute to testicular function and maintenance of male reproductive health, and to the pathologies associated with their abnormal activity in this organ...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29247201/sox30-is-required-for-male-fertility-in-mice
#14
Chun-Wei Allen Feng, Cassy Spiller, Donna J Merriner, Moira K O'Bryan, Josephine Bowles, Peter Koopman
Male infertility is a major and growing problem and, in most cases, the specific root cause is unknown. Here we show that the transcription factor SOX30 plays a critical role in mouse spermatogenesis. Sox30-null mice are healthy and females are fertile, but males are sterile. In the absence of Sox30 meiosis initiates normally in both sexes but, in males, germ cell development arrests during the post-meiotic round spermatid period. In the mutant testis, acrosome and axoneme development are aberrant, multinucleated germ cells (symplasts) form and round spermatids unable to process beyond step 3 of spermiogenesis...
December 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29245011/oncogenic-role-of-thor-a-conserved-cancer-testis-long-non-coding-rna
#15
Yasuyuki Hosono, Yashar S Niknafs, John R Prensner, Matthew K Iyer, Saravana M Dhanasekaran, Rohit Mehra, Sethuramasundaram Pitchiaya, Jean Tien, June Escara-Wilke, Anton Poliakov, Shih-Chun Chu, Sahal Saleh, Keerthana Sankar, Fengyun Su, Shuling Guo, Yuanyuan Qiao, Susan M Freier, Huynh-Hoa Bui, Xuhong Cao, Rohit Malik, Timothy M Johnson, David G Beer, Felix Y Feng, Weibin Zhou, Arul M Chinnaiyan
Large-scale transcriptome sequencing efforts have vastly expanded the catalog of long non-coding RNAs (lncRNAs) with varying evolutionary conservation, lineage expression, and cancer specificity. Here, we functionally characterize a novel ultraconserved lncRNA, THOR (ENSG00000226856), which exhibits expression exclusively in testis and a broad range of human cancers. THOR knockdown and overexpression in multiple cell lines and animal models alters cell or tumor growth supporting an oncogenic role. We discovered a conserved interaction of THOR with IGF2BP1 and show that THOR contributes to the mRNA stabilization activities of IGF2BP1...
December 14, 2017: Cell
https://www.readbyqxmd.com/read/29244804/conditional-knockout-mice-for-the-distal-appendage-protein-cep164-reveal-its-essential-roles-in-airway-multiciliated-cell-differentiation
#16
Saul S Siller, Himanshu Sharma, Shuai Li, June Yang, Yong Zhang, Michael J Holtzman, Wipawee Winuthayanon, Holly Colognato, Bernadette C Holdener, Feng-Qian Li, Ken-Ichi Takemaru
Multiciliated cells of the airways, brain ventricles, and female reproductive tract provide the motive force for mucociliary clearance, cerebrospinal fluid circulation, and ovum transport. Despite their clear importance to human biology and health, the molecular mechanisms underlying multiciliated cell differentiation are poorly understood. Prior studies implicate the distal appendage/transition fiber protein CEP164 as a central regulator of primary ciliogenesis; however, its role in multiciliogenesis remains unknown...
December 15, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29240449/mir-19-5p-enhances-tumorigenesis-in-human-colorectal-cancer-cells-by-targeting-tspyl5
#17
Chao Huang, Hesheng Luo
The cancer suppressor gene, testis-specific protein Y-encoded-like 5 (TSPYL5), has been implicated in various cancers, including colorectal cancer (CRC). In this study, we investigated the role of TSPYL5 in the development of CRC in vitro. First, we used bioinformatics to predict the binding target of TSPYL5, and found that the microRNA, miR-19-5p, bound to the 3' untranslated region (UTR) of TSPYL5. This interaction was further validated by the dual-luciferase assay. Second, we found that overexpressed TSPYL5 enhanced apoptosis in HT29 cells and reduced cell proliferation, reduced cell migration/invasion, and most of the cells accumulated in the G0/G1 phase of the cell cycle...
December 14, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/29237021/human-specific-subcellular-compartmentalization-of-p-element-induced-wimpy-testis-like-piwil-granules-during-germ-cell-development-and-spermatogenesis
#18
Maria Gomes Fernandes, Nannan He, Fang Wang, Liesbeth Van Iperen, Cristina Eguizabal, Roberto Matorras, Bernard A J Roelen, Susana M Chuva De Sousa Lopes
STUDY QUESTION: What is the dynamics of expression of P-element induced wimpy testis-like (PIWIL) proteins in the germline during human fetal development and spermatogenesis? SUMMARY ANSWER: PIWIL1, PIWIL2, PIWIL3 and PIWIL4 were expressed in a sex-specific fashion in human germ cells (GC) during development and adulthood. PIWILs showed a mutually exclusive pattern of subcellular localization. PIWILs were present in the intermitochondrial cement and a single large granule in meiotic GC and their expression was different from that observed in mice, highlighting species-differences...
December 11, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29234547/testicular-cancer-presenting-as-gastric-variceal-hemorrhage
#19
Carlos Eduardo Salazar-Mejía, David Hernández-Barajas, Edio Llerena-Hernández, José Luis González-Vela, María Inés Contreras-Salcido, Adriana González-Gutiérrez, Omar David Borjas-Almaguer, Luis Alberto Pérez-Arredondo, Blanca Otilia Wimer-Castillo
Testicular cancer is the most common solid malignancy affecting males between the ages of 15 and 35. The symptomatology caused by this tumor varies according to the site of metastasis. We present the case of a 26-year-old male who arrived to the emergency department with hematemesis. He had no previous medical history. On arrival, we noted enlargement of the left scrotal sac. There was also a mass in the left scrotum which provoked displacement of the penis and right testis. The serum alpha-fetoprotein level was 17,090 ng/mL, lactate dehydrogenase was 1480 U/L, and human chorionic gonadotropin was 287...
2017: Case Reports in Gastrointestinal Medicine
https://www.readbyqxmd.com/read/29231996/molecular-mechanisms-of-tributyltin-induced-alterations-in-cholesterol-homeostasis-and-steroidogenesis-in-hamster-testis-in-vivo-and-in-vitro-studies
#20
V Kanimozhi, K Palanivel, M A Akbarsha, B Kadalmani
Tributyltins (TBT) are ubiquitous and persistent environmental contaminants that disturb normal endocrine function including gonadal function in humans and marine organisms. TBT was administered through oral route to male Syrian hamsters at daily doses of 50, 100 and 150 ppm/kg for 65 days. Changes in testis morphology, immunohistochemistry of iNOS, 3β-HSD and 17β-HSD, cholesterol transport receptor, nuclear receptors, and transcription factors were analyzed. TBT treatment affected each of these parameters to significant levels in a dose-dependent manner compared to vehicle treated control...
December 12, 2017: Journal of Cellular Biochemistry
keyword
keyword
17906
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"