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https://www.readbyqxmd.com/read/28721614/low-doses-of-chlorpyrifos-interfere-with-spermatogenesis-of-rats-through-reduction-of-sex-hormones
#1
Dinithi Champika Peiris, Thamali Dhanushka
Use of pesticides results in indirect effects on human health. We aimed to evaluate implications of toxicological effects of subchronic chlorpyrifos exposure on reproductive function in male rats. A total of 48 adult Wistar male rats were separated into four groups (n = 12). Animals were gavaged with 2.5 mg/kg (T1), 5 mg/kg (T2), or 10 mg/kg (T3) body weight of chlorpyrifos (CPF) or distilled water (control) daily for 30 days. Organ weights, epididymal sperm parameters, DNA integrity, sex hormonal (FHS and LH) levels, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), and creatinine concentrations were determined on day 31...
July 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28720768/gilgamesh-is-required-for-the-maintenance-of-germline-stem-cells-in-drosophila-testis
#2
Dongsheng Chen, Xiangxiang Zhu, Lijuan Zhou, Jian Wang, Xiaoqian Tao, Shuang Wang, Fuling Sun, Xianzhao Kan, Zhengqi Han, Yuelin Gu
Emerging evidence supports that stem cells are regulated by both intrinsic and extrinsic mechanisms. However, factors that determine the fate of stem cells remain incompletely understood. The Drosophila testis provides an exclusive powerful model in searching for potential important regulatory factors and their underlying mechanisms for controlling the fate of germline stem cells (GSCs). In this study, we have found that Drosophila gilgamesh (gish), which encodes a homologue of human CK1-γ (casein kinase 1-gamma), is required intrinsically for GSC maintenance...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28716148/prevalence-of-plasma-autoantibody-against-cancer-testis-antigen-ny-eso-1-in-htlv-1-infected-individuals-with-different-clinical-status
#3
Yasuo Shiohama, Tadasuke Naito, Toshio Matsuzaki, Reiko Tanaka, Takeaki Tomoyose, Hiroshi Takashima, Takuya Fukushima, Yuetsu Tanaka, Mineki Saito
BACKGROUND: Detection of specific immune responses against cancer/testis antigen NY-ESO-1 was recently reported in patients with adult T-cell leukemia/lymphoma (ATL) and human T-cell leukemia virus type 1 (HTLV-1)-infected asymptomatic carriers (ACs). However, the relationship of the responses with the HTLV-1 proviral load (PVL) and the levels of viral gene expression remain unclear. FINDINGS: We measured plasma levels of autoantibodies to NY-ESO-1 immunogenic tumor antigen in HTLV-1-infected individuals with different clinical status, and in healthy controls...
July 17, 2017: Virology Journal
https://www.readbyqxmd.com/read/28713914/systematic-identification-of-human-housekeeping-genes-possibly-useful-as-references-in-gene-expression-studies
#4
Maria Caracausi, Allison Piovesan, Francesca Antonaros, Pierluigi Strippoli, Lorenza Vitale, Maria Chiara Pelleri
The ideal reference, or control, gene for the study of gene expression in a given organism should be expressed at a medium‑high level for easy detection, should be expressed at a constant/stable level throughout different cell types and within the same cell type undergoing different treatments, and should maintain these features through as many different tissues of the organism. From a biological point of view, these theoretical requirements of an ideal reference gene appear to be best suited to housekeeping (HK) genes...
July 6, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28712647/perinatal-exposure-to-2-2-4-4-tetrabromodiphenyl-ether-induces-testicular-toxicity-in-adult-rats
#5
Ahmed Khalil, Mikhail Parker, Sarah E Brown, Sebnem E Cevik, Lian W Guo, Jake Jensen, Alexandra Olmsted, Daneal Portman, Haotian Wu, Alexander Suvorov
Since 1965, polybrominated diphenyl ethers (PBDEs) have been used internationally as flame-retardant additives. PBDEs were recently withdrawn from commerce in North America and Europe due to their environmental persistence, bioaccumulative properties and endocrine-disrupting effects. Generations exposed perinatally to the highest environmental doses of PBDE account for one-fifth of the total United States population. While, toxicity of PBDE for the male reproductive system has been demonstrated in several human and animal studies, the long-lasting effects of perinatal exposures on male reproduction are still poorly understood...
July 13, 2017: Toxicology
https://www.readbyqxmd.com/read/28711705/bmp15-in-catfish-testis-cellular-distribution-seasonal-variation-and-its-role-in-steroidogenesis
#6
Himanshu Yadav, Bechan Lal
Considering the absence of information on testicular growth factors in fishes, present study was aimed to elucidate the existence of BMP15, an important member of TGF-β superfamily, in the testis of a seasonally breeding freshwater catfish, Clarias batrachus and its role in regulation of testicular activities. The study demonstrated the expression of BMP15 in the somatic cells (Sertoli and interstitial cells) in fish testis. The expression varied with changing testicular activity; the expression was very high in the quiescent and early recrudescing testis coinciding with the renewal of spermatogonial cells...
July 12, 2017: Steroids
https://www.readbyqxmd.com/read/28710511/cerebellar-degeneration-related-proteins-2-and-2-like-are-present-in-ovarian-cancer-in-patients-with-and-without-yo-antibodies
#7
Margrethe Raspotnig, Mette Haugen, Maria Thorsteinsdottir, Ingunn Stefansson, Helga B Salvesen, Anette Storstein, Christian A Vedeler
BACKGROUND: Cerebellar degeneration-related protein 2 (CDR2) has been presumed to be the main antigen for the onconeural antibody Yo, which is strongly associated with ovarian cancer and paraneoplastic cerebellar degeneration (PCD). Recent data show that Yo antibodies also target the CDR2-like protein (CDR2L). We, therefore, examined the expression of CDR2 and CDR2L in ovarian cancer tissue from patients with and without Yo antibodies and from various other cancerous and normal human tissues...
July 14, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28707023/oxidative-inactivation-of-the-endogenous-antioxidant-protein-dj-1-by-the-food-contaminants-3-mcpd-and-2-mcpd
#8
Thorsten Buhrke, Linn Voss, Anja Briese, Heike Stephanowitz, Eberhard Krause, Albert Braeuning, Alfonso Lampen
3-Chloro-1,2-propanediol (3-MCPD) and 2-chloro-1,3-propanediol (2-MCPD) are heat-induced food contaminants being present either as free substances or as fatty acid esters in numerous foods. 3-MCPD was classified to be possibly carcinogenic to humans (category 2B) with kidney and testis being the primary target organs according to animal studies. A previous 28-day oral feeding study with rats revealed that the endogenous antioxidant protein DJ-1 was strongly deregulated at the protein level in kidney, liver, and testis of the experimental animals that had been treated either with 3-MCPD, 2-MCPD or their dipalmitate esters...
July 13, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28701892/a-pilot-study-of-post-operative-adjuvant-vaccine-for-advanced-gastric-cancer
#9
Yoshiyuki Fujiwara, Keijiro Sugimura, Hiroshi Miyata, Takeshi Omori, Hiroyuki Nakano, Chie Mochizuki, Katsuji Shimizu, Hiroaki Saito, Keigo Ashida, Soichiro Honjyo, Yusuke Nakamura, Masahiko Yano
BACKGROUND: Previously, we had performed a clinical study using human leukocyte antigen (HLA)-A24-binding peptide vaccines containing a combination of novel cancer-testis antigens and anti-angiogenic peptides derived from DEPDC1, URLC10, FOXM1, KIF20A and VEGFR1 for advanced gastric cancer (AGC) patients who were refractory to chemotherapy. We applied the cocktail vaccine to the combination therapy with S-1 for patients with AGC as a post-operative adjuvant therapy and performed this clinical pilot study...
June 2017: Yonago Acta Medica
https://www.readbyqxmd.com/read/28688488/support-for-taverna-workflows-in-the-vph-share-cloud-platform
#10
Marek Kasztelnik, Ernesto Coto, Marian Bubak, Maciej Malawski, Piotr Nowakowski, Juan Arenas, Alfredo Saglimbeni, Debora Testi, Alejandro F Frangi
BACKGROUND AND OBJECTIVE: To address the increasing need for collaborative endeavours within the Virtual Physiological Human (VPH) community, the VPH-Share collaborative cloud platform allows researchers to expose and share sequences of complex biomedical processing tasks in the form of computational workflows. The Taverna Workflow System is a very popular tool for orchestrating complex biomedical & bioinformatics processing tasks in the VPH community. This paper describes the VPH-Share components that support the building and execution of Taverna workflows, and explains how they interact with other VPH-Share components to improve the capabilities of the VPH-Share platform...
July 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28680856/sertoli-cells-are-susceptible-to-zikv-infection-in-mouse-testis
#11
Zi-Yang Sheng, Na Gao, Zhao-Yang Wang, Xiao-Yun Cui, De-Shan Zhou, Dong-Ying Fan, Hui Chen, Pei-Gang Wang, Jing An
Flaviviruses including Dengue virus (DENV), Yellow fever virus (YFV), West Nile virus (WNV), and Japanese encephalitis virus (JEV) are global health problems that caused several serious diseases such as fever, hemorrhagic fever, and encephalitis in the past century. Recently, Zika virus (ZIKV) which spreads from Asia to American and causes millions of infections emerges as a new dangerous member of the genus of Flavivirus. Unlike other well-known flaviviruses, ZIKV can be transmitted sexually and infect testes in murine models...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28676534/expression-patterns-of-henmt1-and-piwil1-in-human-testis-implications-for-transposon-expression
#12
Anna-Lena Hempfling, Shu Ly Lim, David Adelson, Jemma Evans, Anne E O'Connor, Zhipeng Qu, Sabine Kliesch, Wolfgang Weidner, M K O'Bryan, Martin Bergmann
This study aimed to define the expression patterns of HENMT1 and PIWI proteins in human testis and investigate their association with transposon expression, infertility sub-type or development of testicular germ cell tumours (TGCT). Testis biopsies showing normal spermatogenesis were used to identify normal localization patterns of HENMT1 and PIWIL1 by immunolocalisation and RT-PCR after laser microdissection. 222 testis biopsies representing normal spermatogenesis, hypospermatogenesis, spermatogenic arrests, Sertoli cell-only (SCO) and testicular germ cell tumours (TGCT) were analysed by RT-qPCR for expression of HENMT1/PIWIL1/PIWIL2/PIWIL3/PIWIL4 and LINE-1...
July 4, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28674937/scaffold-based-and-scaffold-free-testicular-organoids-from-primary-human-testicular-cells
#13
Yoni Baert, Charlotte Rombaut, Ellen Goossens
Organoid systems take advantage of the self-organizing capabilities of cells to create diverse multi-cellular tissue surrogates that constitute a powerful novel class of biological models. Clearly, the formation of a testicular organoid (TO) in which human spermatogenesis can proceed from a single-cell suspension would exert a tremendous impact on research and development, clinical treatment of infertility, and screening of potential drugs and toxic agents. Recently, we showed that primary adult and pubertal human testicular cells auto-assembled in TOs either with or without the support of a natural testis scaffold...
July 4, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28670489/heat-shock-protein-70-2-hsp70-2-a-novel-cancer-testis-antigen-that-promotes-growth-of-ovarian-cancer
#14
Namita Gupta, Nirmala Jagadish, Avadhesha Surolia, Anil Suri
Heat shock protein 70-2 (HSP70-2) is known to be involved in tumor progression. However, its molecular role and mechanism in epithelial ovarian cancer (EOC) remains unknown. In the present investigation, we examined the role of HSP70-2 in cell cycle, apoptosis and epithelial mesenchymal transition pathways in EOC cells in in vitro and in-vivo xenograft mouse model. To investigate the role of HSP70-2 in ovarian cancer, plasmid driven short hairpin RNA approach was used to examine HSP70-2 gene and protein expression in ovarian cancer cell line A-10 (origin: serous papillary cystadenocarcinoma), Caov-3 (origin: adenocarcinoma) and SKOV3 (origin: adenocarcinoma; derived from metastatic site: ascites) by RT-PCR, quantitative-PCR, immunohistochemistry and Western blotting...
2017: American Journal of Cancer Research
https://www.readbyqxmd.com/read/28669898/relevance-of-tissue-specific-subunit-expression-in-channelopathies
#15
REVIEW
Hartwig Seitter, Alexandra Koschak
Channelopathies are a diverse group of human disorders that are caused by mutations in genes coding for ion channels or channel-regulating proteins. Several dozen channelopathies have been identified that involve both non-excitable cells as well as electrically active tissues like brain, skeletal and smooth muscle or the heart. In this review, we start out from the general question which ion channel genes are expressed tissue-selectively. We mined the human gene expression database Human Protein Atlas (HPA) for tissue-enriched ion channel genes and found 85 genes belonging to the ion channel families...
June 29, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28667465/the-role-of-vitamin-d-in-male-fertility-a-focus-on-the-testis
#16
REVIEW
Cristina de Angelis, Mariano Galdiero, Claudia Pivonello, Francesco Garifalos, Davide Menafra, Federica Cariati, Ciro Salzano, Giacomo Galdiero, Mariangela Piscopo, Alfonso Vece, Annamaria Colao, Rosario Pivonello
In the last decade, vitamin D has emerged as a pleiotropic molecule with a multitude of autocrine, paracrine and endocrine functions, mediated by classical genomic as well as non-classical non-genomic actions, on multiple target organs and systems. The expression of vitamin D receptor and vitamin D metabolizing enzymes in male reproductive system, particularly in the testis, suggests the occurrence of vitamin D synthesis and regulation as well as function in the testis. The role of vitamin D in the modulation of testis functions, including hormone production and spermatogenesis, has been investigated in animals and humans...
July 1, 2017: Reviews in Endocrine & Metabolic Disorders
https://www.readbyqxmd.com/read/28667125/induction-of-eao-in-mice-responses-to-elevated-circulating-levels-of-the-follistatin
#17
Nour Nicolas, Julie A Muir, Susan Hayward, Justin L Chen, Peter G Stanton, Paul Gregorevic, David M de Kretser, Kate L Loveland, Sudhanshu Bhushan, Andreas Meinhardt, Monika Fijak, Mark P Hedger
Experimental autoimmune orchitis (EAO) is a rodent model of chronic testicular inflammation that mimics the pathology observed in some types of human infertility. In a previous study, testicular expression of the inflammatory/immunoregulatory cytokine, activin A, was elevated in adult mice during the onset of EAO, indicating a potential role in regulation of the disease. Consequently, we examined the development of EAO in mice with elevated levels of follistatin, an endogenous activin antagonist, as a potential therapeutic approach to testicular inflammation...
June 30, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28646208/a-comprehensive-mouse-transcriptomic-bodymap-across-17-tissues-by-rna-seq
#18
Bin Li, Tao Qing, Jinhang Zhu, Zhuo Wen, Ying Yu, Ryutaro Fukumura, Yuanting Zheng, Yoichi Gondo, Leming Shi
The mouse has been widely used as a model organism for studying human diseases and for evaluating drug safety and efficacy. Many diseases and drug effects exhibit tissue specificity that may be reflected by tissue-specific gene-expression profiles. Here we construct a comprehensive mouse transcriptomic BodyMap across 17 tissues of six-weeks old C57BL/6JJcl mice using RNA-seq. We find different expression patterns between protein-coding and non-coding genes. Liver expressed the least complex transcriptomes, that is, the smallest number of genes detected in liver across all 17 tissues, whereas testis and ovary harbor more complex transcriptomes than other tissues...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28645691/maternal-exposure-to-a-mixture-of-persistent-organic-pollutants-pops-affects-testis-histology-epididymal-sperm-count-and-induces-sperm-dna-fragmentation-in-mice
#19
Abdolrahman Khezri, Birgitte Lindeman, Anette K Krogenæs, Hanne F Berntsen, Karin E Zimmer, Erik Ropstad
Persistent organic pollutants (POPs) are widespread throughout the environment and some are suspected to induce reproductive toxicity. As animals and humans are exposed to complex mixtures of POPs, it is reasonable to assess how such mixtures could interact with the reproductive system. Our aim is to investigate how maternal exposure to a mixture of 29 different persistent organic pollutants, formulated to mimic the relative POP levels in the food basket of the Scandinavian population, could alter reproductive endpoints...
June 20, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28643774/insufficient-antibody-validation-challenges-oestrogen-receptor-beta-research
#20
Sandra Andersson, Mårten Sundberg, Nusa Pristovsek, Ahmed Ibrahim, Philip Jonsson, Borbala Katona, Carl-Magnus Clausson, Agata Zieba, Margareta Ramström, Ola Söderberg, Cecilia Williams, Anna Asplund
The discovery of oestrogen receptor β (ERβ/ESR2) was a landmark discovery. Its reported expression and homology with breast cancer pharmacological target ERα (ESR1) raised hopes for improved endocrine therapies. After 20 years of intense research, this has not materialized. We here perform a rigorous validation of 13 anti-ERβ antibodies, using well-characterized controls and a panel of validation methods. We conclude that only one antibody, the rarely used monoclonal PPZ0506, specifically targets ERβ in immunohistochemistry...
June 15, 2017: Nature Communications
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