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https://www.readbyqxmd.com/read/29142545/rapid-screening-of-pyogenic-staphylococcus-aureus-for-confirmation-of-genus-and-species-methicillin-resistance-and-virulence-factors-by-using-two-novel-multiplex-pcr
#1
Abdul Haque, Asma Haque, Muhammad Saeed, Aysha Azhar, Samreen Rasool, Sidra Shan, Beenish Ehsan, Zohaib Nisar
Objectives: Emergence of methicillin resistant Staphylococcus aureus (MRSA) is a major medical problem of current era. These bacteria are resistant to most drugs and rapid diagnosis can provide a clear guideline to clinicians. They possess specific virulence factors and relevant information can be very useful. We designed this study to develop multiplex PCRs to provide rapid information. Methods: We studied 60 Staphylococcus aureus isolates and detected methicillin resistance by cefoxitin sensitivity and targeting of mecA gene...
September 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29124834/prevalence-and-characterization-of-staphylococcus-aureus-causing-community-acquired-skin-and-soft-tissue-infections-on-java-and-bali-indonesia
#2
Dewi Santosaningsih, Sanarto Santoso, Nanik Setijowati, Harun A Rasyid, Nyoman S Budayanti, Ketut Suata, Dicky B Widhyatmoko, Priyo B Purwono, Kuntaman Kuntaman, Damayanti Damayanti, Cita R S Prakoeswa, Mitchell Laurens, Josephine W I van Nierop, Geraldine L Nanninga, Neline Oudenes, Michelle de Regt, Susan V Snijders, Henri A Verbrugh, Juliëtte A Severin
OBJECTIVES: To define the role of Staphylococcus aureus in community settings among patients with skin and soft tissue infections (SSTI) in Indonesia. METHODS: S. aureus were cultured from anterior nares, throat, and wounds of 567 ambulatory patients presenting with SSTI. The mecA gene and genes encoding Panton-Valentine leukocidin (PVL; lukF-PV and lukS-PV) and exfoliative toxin (ET; eta and etb) were determined by PCR. Clonal relatedness among methicillin-resistant S...
November 10, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/29117225/genomic-comparison-between-staphylococcus-aureus-gn-strains-clinically-isolated-from-a-familial-infection-case-is1272-transposition-through-a-novel-inverted-repeat-replacing-mechanism
#3
Tsai-Wen Wan, Wataru Higuchi, Olga E Khokhlova, Wei-Chun Hung, Yasuhisa Iwao, Masataka Wakayama, Noriyoshi Inomata, Tomomi Takano, Yu-Tzu Lin, Olga V Peryanova, Kenji K Kojima, Alla B Salmina, Lee-Jene Teng, Tatsuo Yamamoto
A bacterial insertion sequence (IS) is a mobile DNA sequence carrying only the transposase gene (tnp) that acts as a mutator to disrupt genes, alter gene expressions, and cause genomic rearrangements. "Canonical" ISs have historically been characterized by their terminal inverted repeats (IRs), which may form a stem-loop structure, and duplications of a short (non-IR) target sequence at both ends, called target site duplications (TSDs). The IS distributions and virulence potentials of Staphylococcus aureus genomes in familial infection cases are unclear...
2017: PloS One
https://www.readbyqxmd.com/read/29115975/pvl-overexpression-due-to-genomic-rearrangements-and-mutations-in-the-s-aureus-reference-strain-atcc25923
#4
Bettina Stieber, Artur Sabat, Stefan Monecke, Peter Slickers, Viktoria Akkerboom, Elke Müller, Alexander W Friedrich, Ralf Ehricht
OBJECTIVE: ATCC25923 is a Staphylococcus aureus strain that is positive for the Panton Valentin leukocidin. It has been used for decades as reference strain. We observed that two separately maintained clones of ATCC25923 ("G477 and G478") differed grossly in the expression of this toxin. For that reason, both clones were sequenced using an Illumina MiSeq instrument. After assembling, the final sequences were analyzed and mapped to a previously published ATCC25923 sequence (GenBank CP009361) using bl2seq from the NCBI Blast2 package...
November 7, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29084288/closely-related-methicillin-resistant-staphylococcus-aureus-isolates-from-retail-meat-cows-with-mastitis-and-humans-in-japan
#5
Tomomi Sato, Masaru Usui, Noriko Konishi, Akemi Kai, Hidehito Matsui, Hideaki Hanaki, Yutaka Tamura
Methicillin-resistant Staphylococcus aureus (MRSA) is a pervasive healthcare-acquired (HA) pathogen with recent emergence as a community-acquired (CA) pathogen. To elucidate whether meat mediates MRSA transmission between animals and humans in Japan, this study examined MRSA isolates from retail meat (n = 8), cows with mastitis (n = 7), and humans (HA-MRSA = 46 and CA-MRSA = 54) by molecular typing, virulence gene analyses, and antimicrobial susceptibility testing. MRSA isolates from retail meat were classified into sequence type (ST) 8/spa type t1767 (n = 4), ST8/t4133 (n = 1), ST59/t3385 (n = 1), ST88/t375 (n = 1), and ST509/t375 (n = 1)...
2017: PloS One
https://www.readbyqxmd.com/read/29068720/detection-and-molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-from-table-eggs-in-haripur-pakistan
#6
Muhammad Ali Syed, Syeda Hala Hussain Shah, Yasmin Sherafzal, Syed Shafi-Ur-Rehman, Mushtaq Ahmed Khan, John B Barrett, Tiffanie A Woodley, Bushra Jamil, Shahid Ahmad Abbasi, Charlene R Jackson
Table eggs are nutritionally important food consumed globally. Despite being protected inside the hard shell and a semipermeable membrane, the egg contents may be contaminated with microbes and thus become a possible carrier of infectious agents to humans. A number of medically significant bacterial species such as Salmonella enterica, Listeria monocytogenes, and Yersinia enterocolitica have already been reported from table eggs. More important is the presence of antimicrobial-resistant bacterial strains in this food source...
October 25, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29048488/clinical-and-molecular-features-of-mdr-livestock-associated-mrsa-st9-with-staphylococcal-cassette-chromosome-mecxii-in-humans
#7
Chih-Jung Chen, Tsai-Ling Yang Lauderdale, Chin-Te Lu, Yu-Yu Chuang, Chien-Chang Yang, Ting-Shu Wu, Chun-Yi Lee, Min-Chi Lu, Wen-Chien Ko, Yhu-Chering Huang
Objectives: Clonal complex (CC) 9 is a prevalent livestock-associated (LA) MRSA clone in Asia whose pathogenicity in humans remains unknown. Methods: In 2012, we identified a patient with CC9-MRSA infection linked to livestock. After screening 3328 clinical MRSA isolates from a national database, eight isolates (0.24%) collected between 1998 and 2012 were further confirmed to be of CC9. The detailed molecular features of the nine human CC9 strains and phylogenetic relatedness to animal CC9 strains were characterized with WGS...
October 18, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28956538/epidemiology-of-panton-valentine-leukocidin-harbouring-staphylococcus-aureus-in-cutaneous-infections-from-iran-a-systematic-review-and-meta-analysis
#8
Milad Shahini Shams Abadi, Iraj Nikokar, Seyedeh Mahsan Hoseini Alfatemi, Yalda Malekzadegan, Abdolah Azizi, Hadi Sedigh Ebrahim-Saraie
Panton-Valentine Leukocidin (PVL) producing Staphylococcus aureus has been associated with severity of skin infections and pathology that suggest a major role in pathogenicity. The present study aimed to determine the overall prevalence of PVL harbouring S. aureus isolates from cutaneous infections in Iran. A systematic search was performed by using Medline electronic databases (PubMed) from the papers published by Iranian authors to the end of March 2017. Ten publications which met our inclusion criteria were then selected for data extraction and analysis by Comprehensive Meta-Analysis Software...
September 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/28946704/emergence-of-nasal-carriage-of-st80-and-st152-pvl-staphylococcus-aureus-isolates-from-livestock-in-algeria
#9
Amir Agabou, Zouleikha Ouchenane, Christelle Ngba Essebe, Salim Khemissi, Mohamed Tedj Eddine Chehboub, Ilyes Bey Chehboub, Albert Sotto, Catherine Dunyach-Remy, Jean-Philippe Lavigne
The spread of toxinogenic Staphylococcus aureus is a public health problem in Africa. The objectives of the study were to investigate the rate of S. aureus nasal carriage and molecular characteristics of these strains in livestock and humans in three Algerian provinces. Nasal samples were collected from camels, horses, cattle, sheep and monkeys, as well as humans in contact with them. S. aureus isolates were genotyped using DNA microarray. The rate of S. aureus nasal carriage varied between species: camels (53%), humans and monkeys (50%), sheep (44...
September 25, 2017: Toxins
https://www.readbyqxmd.com/read/28938255/scabies-periorbital-cellulitis-and-recurrent-skin-abscesses-due-to-panton-valentine-leukocidin-positive-staphylococcus-aureus-mimic-hyper-ige-syndrome-in-an-infant
#10
Renate Krüger, Leif G Hanitsch, Rasmus Leistner, Sylke Schneider-Burrus, Pia-Alice Hoppe, Sylvia Steinberg, Fabian Hauck, Horst von Bernuth
We describe the clinical course of a 2-month-old infant who was evaluated for autosomal dominant Hyper IgE Syndrome based on eczema, periorbital cellulitis, skin abscesses, increased total IgE levels and blood eosinophilia. However, scabies and nasal colonization by Panton-Valentine Leucocidin-positive S. aureus were eventually diagnosed. After specific treatment, the child was asymptomatic.
December 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28935494/-an-atypical-case-of-necrotizing-staphylococcal-pneumonia
#11
E Bertreau, S Grard, T Baudry, N Freymond
INTRODUCTION: Some strains of Staphylococcus aureus produce a toxin known as Panton-Valentine leukocidin. These strains notably cause a necrotizing pneumonia which is associated with a high mortality. OBSERVATION: A 70-year-old woman presented with sub-acute onset dyspnea, low-grade fever, and hemoptysis after a trip to Dubai and New Zealand. Computed tomography showed bilateral necrotizing pneumonia, suggesting the diagnosis of pneumonia caused by S. aureus producing Panton-Valentine toxin...
September 18, 2017: Revue des Maladies Respiratoires
https://www.readbyqxmd.com/read/28904790/prevalence-of-methicillin-resistant-staphylococcus-aureus-mrsa-in-retail-food-in-singapore
#12
Kyaw Thu Aung, Li Yang Hsu, Tse Hsien Koh, Hapuarachchige Chanditha Hapuarachchi, Man Ling Chau, Ramona Alikiiteaga Gutiérrez, Lee Ching Ng
We characterised 227 Staphylococcus aureus isolates from retail food and food handlers' gloves samples obtained through food surveillance and risk assessment studies between 2011 and 2014. Of 227 isolates, five (2.2%) were methicillin-resistant and belonged to sequence types ST80 (n = 3) and ST6 (n = 2). All five isolates belonged to SCCmec type IV, were Panton-Valentine leukocidin (pvl)-negative and staphylococcal enterotoxin genes-positive. Resistance to azithromycin was found in ST80 isolates, in addition to resistance to beta-lactams...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28900424/the-impact-of-the-staphylococcus-aureus-virulome-on-infection-in-a-developing-country-a-cohort-study
#13
Marthe Lebughe, Patrick Phaku, Silke Niemann, Dieudonné Mumba, Georg Peters, Jean-Jacques Muyembe-Tamfum, Alexander Mellmann, Lena Strauß, Frieder Schaumburg
We performed a cohort study to analyze the virulome of Staphylococcus aureus from the Democratic Republic of the Congo using whole genome sequencing and to assess its impact on the course of S. aureus infections. Community-associated S. aureus from nasal colonization (n = 100) and infection (n = 86) were prospectively collected. Phenotypic susceptibility testing and WGS was done for each isolate. WGS data were used to screen for 79 different virulence factors and for genotyping purposes (spa typing, multilocus sequence typing)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28892893/distribution-of-sccmec-elements-and-presence-of-panton-valentine-leukocidin-in-methicillin-resistant-staphylococcusepidermidis-isolated-from-clinical-samples-in-a-university-hospital-of-isfahan-city-iran
#14
Mehrdad Halaji, Ashkan Karimi, Parisa Shoaei, Mohammadreza Nahaei, Farzin Khorvash, Behrooz Ataei, Majid Yaran, Seyed Asghar Havaei
INTRODUCTION: Coagulase Negative Staphylococcus (CoNS) is considered as a major pathogen of nosocomial infections among immunosuppressed patients. AIM: The aim of this study was to identify the types of Staphylococcal Cassette Chromosome mec (SCCmec) and Panton-Valentine Leukocidin (PVL) gene among clinical Methicillin-Resistant S. epidermidis (MRSE) isolates collected from Isfahan. MATERIALS AND METHODS: This cross-sectional study was performed from March 2014 to January 2015 at a tertiary care hospital of Isfahan, Iran...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28886942/remitting-infections-due-to-community-acquired-panton-valentine-leukocidin-producing-staphylococcus-aureus-in-the-milan-area
#15
Sara Giordana Rimoldi, Cristina Pagani, Erika Longhi, Valentina Di Cristo, Annamaria Di Gregorio, Alessandro Mancon, Pietro Zerbi, Cristina Gervasoni, Maria Rita Gismondo, Agostino Riva
One of the most important Staphylococcus aureus virulence factors is Panton-Valentine leukocidin (PVL). We describe an outbreak of recurrent cutaneous PVL infections in different members of three family clusters. Molecular investigations were performed to confirm the presence of the mecA and PVL genes and to assign the SCCmec type, sequence type (ST) and clonal relatedness. A strain of PVL-producing methicillin-resistant S. aureus (MRSA) was responsible for infection in two related families (A and B), and a third family (C) was infected with PVL-producing methicillin-sensitive S...
September 5, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28869541/spread-of-tst-positive-staphylococcus-aureus-strains-belonging-to-st30-clone-among-patients-and-healthcare-workers-in-two-intensive-care-units
#16
Matthaios Papadimitriou-Olivgeris, Eleanna Drougka, Fotini Fligou, Vasiliki Dodou, Fevronia Kolonitsiou, Kriton S Filos, Evangelos D Anastassiou, Efthimia Petinaki, Markos Marangos, Iris Spiliopoulou
Staphylococcus aureus is a major cause of infections. Toxic shock syndrome toxin (TSST-1) and Panton-Valentine leukocidin (PVL) are associated with severe clinical syndromes. S. aureus colonizing isolates recovered from healthcare workers and patients in the intensive care unit (ICU) of a university hospital comprising Group A were compared with those from adult non-ICU carriers (Group B). mecA, lukS/lukF-PV (Panton-Valentine leukocidin, PVL), and tst (toxic shock syndrome toxin) gene carriage was detected by PCR...
September 4, 2017: Toxins
https://www.readbyqxmd.com/read/28866692/frequency-and-molecular-epidemiology-of-panton-valentine-leukocidin-gene-in-staphylococcus-aureus-colonising-hiv-infected-patients
#17
Z Mohd-Zain, S F A Mohd-Nawi, A Adnan, S Kumar
BACKGROUND: HIV-infected patients pose a high risk of contracting skin and soft tissue infections caused by Staphylococcus aureus. Those who are colonized with methicillin-resistant S. aureus (MRSA) that carry Panton-Valentine leukocidin (PVL) are predisposed to severe infections that could lead to necrotic skin infections. However the association of S. aureus specifically methicillin sensitive S. aureus carrying PVL gene in HIV patients has not been widely reported. Here, we study the prevalence and the molecular epidemiology of PVL-producing S...
August 2017: Malaysian Journal of Pathology
https://www.readbyqxmd.com/read/28865851/prevalence-antimicrobial-susceptibility-and-molecular-characterization-of-staphylococcus-aureus-isolated-from-dairy-herds-in-northern-china
#18
Huimin Liu, Songli Li, Lu Meng, Lei Dong, Shengguo Zhao, Xinyi Lan, Jiaqi Wang, Nan Zheng
Staphylococcus aureus is one of the main pathogens involved in dairy cow mastitis. Monitoring of antibiotic use would prove useful to assess the risk of Staph. aureus in raw milk. The objective of this work was to investigate the prevalence of Staph. aureus strais isolated from raw milk in northern China, and to characterize antimicrobial susceptibility of these strains and their key virulence genes. In total, 195 raw milk samples were collected from 195 dairy farms located in 4 cities of northern China from May to September 2015...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28859499/prevalence-and-molecular-typing-of-methicillin-resistant-staphylococcus-aureus-carrying-panton-valentine-leukocidin-gene
#19
Maryam Rahimpour Hesari, Ali Salehzadeh, Reza Kazemi Darsanaki
Panton-Valentine leukocidin (pvl) toxin is an important virulence factor of Staphylococcus aureus. The main genes are coa and spa for distinguishing and typing of S. aureus isolates. The aim of this study was to investigate antibiotic resistance, presence of mecA and pvl genes, as well as epidemiological typing of these isolates according to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in clinical sample isolated from Rasht city, Iran. A total of 250 clinical samples have been isolated from different hospitals...
September 1, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28834892/the-association-between-staphylococcus-aureus-nasal-colonization-and-symptomatic-infection-in-children-in-korea-where-st72-is-the-major-genotype-a-prospective-observational-study
#20
Sunghan Kang, Jina Lee, Mina Kim
This study was performed to investigate the concordance in terms of molecular characteristics and antimicrobial susceptibility between colonizing and clinical Staphylococcus aureus isolates obtained from children in Korea, where ST72 is the major genotype.This was a prospective observational descriptive study of culture-confirmed S aureus infections obtained from children ≤18 years old admitted to Asan Medical Center Children's Hospital in Seoul, Korea, from March 2014 to April 2015. Molecular studies including multilocus sequence typing (MLST), SCCmec typing, polymerase chain reaction amplification of the Panton-Valentine leukocidin (PVL) genes, and antibiotic susceptibility tests were performed on S aureus isolates obtained from nares and clinical specimens...
August 2017: Medicine (Baltimore)
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