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https://www.readbyqxmd.com/read/28644873/circular-rna-expression-profiling-of-human-granulosa-cells-during-maternal-aging-reveals-novel-transcripts-associated-with-assisted-reproductive-technology-outcomes
#1
Jing Cheng, Jia Huang, Suzhen Yuan, Su Zhou, Wei Yan, Wei Shen, Yun Chen, Xi Xia, Aiyue Luo, Da Zhu, Shixuan Wang
Circular RNAs (circRNAs) are a unique class of endogenous RNAs which could be used as potential diagnostic and prognostic biomarkers of many diseases. Our study aimed to investigate circRNA profiles in human granulosa cells (GCs) during maternal aging and to uncover age-related circRNA variations that potentially reflect decreased oocyte competence. CircRNAs in GCs from in vitro fertilization (IVF) patients with young age (YA, ≤ 30 years) and advanced age (AA, ≥ 38 years) were profiled by microarray, and validated in 20 paired samples...
2017: PloS One
https://www.readbyqxmd.com/read/28644353/extraintestinal-manifestations-of-celiac-disease-effectiveness-of-the-gluten-free-diet
#2
Hilary Jericho, Naire Sansotta, Stefano Guandalini
OBJECTIVE: The aim of the study was to evaluate the effectiveness of the gluten-free diet (GFD) on extraintestinal symptoms in pediatric and adult celiac populations at the University of Chicago. METHODS: We conducted a retrospective chart review of the University of Chicago Celiac Center clinic charts from January 2002 to October 2014. Demographics, serologic testing, intestinal biopsies, and extraintestinal symptoms at presentation, 12, 24, and >24 months were recorded...
July 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28643089/time-lapse-observation-and-transcriptome-analysis-of-a-case-with-repeated-multiple-pronuclei-after-ivf-icsi
#3
J Dai, L Z Leng, C F Lu, F Gong, S P Zhang, W Zheng, G X Lu, G Lin
PURPOSE: The purpose of this study was to investigate the cause of repeated multipronucleus (MPN) formation in zygotes in a patient after both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). METHOD: This is a case study. A patient had unexplained primary infertility with recurring total MPN zygotes after IVF and ICSI cycles. Time-lapse monitoring of pronucleus formation was carried out. Embryos developed from MPN zygotes were analyzed by fluorescence in situ hybridization (FISH)...
June 22, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28641947/effectiveness-of-human-spermatozoa-biomarkers-as-indicators-of-structural-damage-during-cryopreservation
#4
María José Gómez-Torres, Llanos Medrano, Alejandro Romero, Pedro José Fernández-Colom, Jon Aizpurúa
Human spermatozoa cryopreservation techniques are used to maintain and protect male fertility in cases such as infertility and malignancy treatments. However, during cryopreservation, the spermatozoa's metabolic rate is reduced and they undergo dramatic functional and structural changes owing to exposure to cryoprotectants and freezing-thawing procedures. While the effects of cryopreservation on cells are documented, to date the induced cryodamage on structural and/or functional sperm biomarkers is not well established at multivariate scale...
June 19, 2017: Cryobiology
https://www.readbyqxmd.com/read/28641583/prevalence-and-risk-factors-of-infertility-in-turkey-evidence-from-demographic-and-health-surveys-1993-2013
#5
Melike Sarac, Ismet Koc
The inability to have children affects couples worldwide and causes emotional and psychological distress in both men and women. Turkey is a country that lays particular emphasis on the issue of infertility, especially after experiencing a dramatic fertility decline over the last two decades. This study aimed to understand the changes in the prevalence of infertility in Turkey using three different approaches: the DHS Approach, the Constructed Approach and the Current Duration Approach. Furthermore, the factors contributing to elevated risks of infertility as derived from the Constructed Approach were investigated using four different logistic regression models...
June 23, 2017: Journal of Biosocial Science
https://www.readbyqxmd.com/read/28641096/hematopoietic-stem-cell-transplantation-for-sickle-cell-anemia-the-changing-landscape
#6
REVIEW
Adetola A Kassim, Deva Sharma
Allogeneic hematopoietic stem cell transplantation (HSCT) remains the only curative therapy for sickle cell disease (SCD); however, its use is limited by lack of suitable human leukocyte antigen (HLA)-matched donors and decreased application in older patients with significant morbidity. Myeloablative, HLA-identical sibling transplantation in children with SCD offers excellent long-term survival, with overall and event-free survival rates of 95% and 92%, respectively. However, the risk of graft-versus-host-disease, infections, infertility, and other long-term transplant complications, further limits its widespread use...
June 15, 2017: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/28640964/targeted-mutagenesis-in-tetraploid-switchgrass-panicum-virgatum-l-using-crispr-cas9
#7
Yang Liu, Paul Merrick, Zhengzhi Zhang, Chonghui Ji, Bing Yang, Shui-Zhang Fei
The CRISPR/Cas9 system has become a powerful tool for targeted mutagenesis. Switchgrass (Panicum virgatum L.) is a high yielding perennial grass species that has been designated as a model biomass crop by the U.S. Department of Energy. The self infertility and high ploidy level make it difficult to study gene function or improve germplasm. To overcome these constraints, we explored the feasibility of using CRISPR/Cas9 for targeted mutagenesis in a tetraploid cultivar 'Alamo' switchgrass. We first developed a transient assay by which a non-functional green fluorescent protein gene containing a 1 bp frameshift insertion in its 5' coding region was successfully mutated by a Cas9/sgRNA complex resulting in its restored function...
June 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28638960/comparative-diagnostic-evaluation-of-omp31-gene-based-taqman%C3%A2-real-time-pcr-assay-with-visual-lamp-assay-and-indirect-elisa-for-caprine-brucellosis
#8
Suman Saini, V K Gupta, K Gururaj, D D Singh, R V S Pawaiya, N K Gangwar, A K Mishra, Deepak Dwivedi, Dimple Andani, Ashok Kumar, T K Goswami
Brucellosis is one of the leading causes of abortion in domestic animals that imposes costs on both economy and society. The disease is highly zoonotic and poses risk to animal handlers due to its zoonotic nature. It causes stillbirth, loss of kids and abortion in last term of pregnancy. Reproductive damage includes infertility in does and orchitis and epididymitis in breeding bucks, which result in high financial losses to farmers and the agriculture industry as a whole. It requires highly sensitive and specific assays to diagnose the disease at field level...
June 21, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28638413/uterine-cavity-abnormalities-in-patients-with-endometriosis-in-alexandria-a-diagnostic-test-accuracy-study
#9
Eman Aly Abd El Fattah
Endometriosis is strongly associated with infertility. Endometrial polyps are prevalent in infertile women and they have similar pathological characteristics to endometriosis, suggesting a possible association. Uterine malformations as uterine septum and hypoplastic uterus are also linked to endometriosis. Hysterosalpingogram and transvaginal ultrasonography are used to diagnose endometrial lesions. Hysteroscopy can detect small lesions that might be missed. Recently, 4D ultrasonography is being used, but which is superior has not been established yet...
2017: Obstetrics and Gynecology International
https://www.readbyqxmd.com/read/28638367/gnrh-agonist-trigger-and-lh-activity-luteal-phase-support-versus-hcg-trigger-and-conventional-luteal-phase-support-in-fresh-embryo-transfer-ivf-icsi-cycles-a-systematic-prisma-review-and-meta-analysis
#10
REVIEW
Thor Haahr, Matheus Roque, Sandro C Esteves, Peter Humaidan
INTRODUCTION: The use of GnRH agonist (GnRHa) for final oocyte maturation trigger in oocyte donation and elective frozen embryo transfer cycles is well established due to lower ovarian hyperstimulation syndrome (OHSS) rates as compared to hCG trigger. A recent Cochrane meta-analysis concluded that GnRHa trigger was associated with reduced live birth rates (LBRs) in fresh autologous IVF cycles compared to hCG trigger. However, the evidence is not unequivocal, and recent trials have found encouraging reproductive outcomes among couples undergoing GnRHa trigger and individualized luteal LH activity support...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28638213/comment-on-disability-psychiatric-symptoms-and-quality-of-life-in-infertile-women-a-cross-sectional-study-in-turkey
#11
EDITORIAL
Salvatore Giovanni Vitale, Valentina Lucia La Rosa, Agnese Maria Chiara Rapisarda, Antonio Simone Laganà
The aim of this work is to comment on the study about disability, psychiatric symptoms and quality of life in infertile women of Turkey that was published in this Journal. We suggest that future studies use larger samples, consider the influence of factors such as cause of infertility and assisted reproductive techniques (ARTs) and, last but not least, exclude other comorbidities which may influence the data analysis.
December 25, 2016: Shanghai Archives of Psychiatry
https://www.readbyqxmd.com/read/28638108/pregnancy-outcomes-in-nulliparous-women-after-ultrasound-ablation-of-uterine-fibroids-a-single-central-retrospective-study
#12
Jun Shu Li, Yong Wang, Jin Yun Chen, Wen Zhi Chen
To explore the impact of high-intensity focused ultrasound (HIFU) ablation of uterine fibroids in nulliparous women on subsequent pregnancy and delivery. A retrospective analysis was conducted of nulliparous women who received HIFU treatment at Chongqing Medical University, Chongqing,China, from January 1, 2010, to January 1, 2015. A total of 189 cases were enrolled, the median follow-up time was three years. Among them,there were 131 cases pregnancy with a total of 133 times,the pregnancy rate was 69.3% (131/189),and the spontaneous conception rate was 95...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28638075/mycobacterial-heat-shock-protein-65-mediated-metabolic-shift-in-decidualization-of-human-endometrial-stromal-cells
#13
Elavarasan Subramani, Arun Prabhu Rameshbabu, Manivannan Jothiramajayam, Bhuvaneshwaran Subramanian, Debangana Chakravorty, Gunja Bose, Mamata Joshi, Chaitali Datta Ray, Indrani Lodh, Ratna Chattopadhyay, Sudipto Saha, Anita Mukherjee, Santanu Dhara, Baidyanath Chakravarty, Koel Chaudhury
Successful implantation is dependent on the appropriate decidualization of endometrial stromal cells for the establishment of pregnancy in women. Mycobacterial heat shock protein 65 (HSP65) is involved in pathogenesis of the genital tuberculosis (GTB), one of the common causes of infertility in emerging countries. Though implantation failure appears to be the major cause, understanding the status of decidualizaiton process in women diagnosed with GTB has not been thoroughly addressed. We, therefore, explored the effect of HSP65 protein on the endometrial cell metabolism during in vitro decidualization...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28637085/successful-delivery-derived-from-cryopreserved-rare-human-spermatozoa-with-novel-cryopiece
#14
J Sun, W Chen, L Zhou, J Hu, Z Li, Z Zhang, Y Wu
Herein, we report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with cryopiece cryopreserved rare human spermatozoa from severe male factor infertility patients. We established a novel cryopiece system on the basis of previous studies. In this study, 126 spermatozoa from four patients with non-obstructiveazoospermia (NOA) or severe oligozoospermia were stored in cryopiece and then thawed on the day of the oocyte retrieval, 88 (83%) spermatozoa were recovered with a 47.5% (38 of 80) motile rate...
June 21, 2017: Andrology
https://www.readbyqxmd.com/read/28636237/microperforate-hymen-presenting-with-incomplete-abortion-a-case-report
#15
Maya Padhi, Priyadarshini Tripathy, Asutosh Sahu
A 20-year-old patient with 3 months of amenorrhea presented in the labor room with bleeding per vaginum and having experienced lower abdominal pain for 2 h. She had a history of difficult intercourse and prolonged menstrual flow. Inspection revealed a bleeding point over an obstructing membrane at the level of the vaginal introitus. Subsequent vaginal examination confirmed the diagnosis of microperforate hymen. Her urine pregnancy test was positive and an ultrasound examination revealed the presence of retained products of conception in the endometrial cavity...
June 21, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28635535/effect-of-the-previous-reproductive-outcomes-in-subfertile-women-after-in-vitro-fertilization-ivf-and-or-intracytoplasmic-sperm-injection-icsi-treatments-on-perinatal-anxious-and-depressive-symptomatology
#16
Francesca Agostini, Fiorella Monti, Marcella Paterlini, Federica Andrei, Stefano Palomba, Giovanni Battista La Sala
INTRODUCTION: The present study aims to investigate the effects of previous reproductive outcomes on the levels of depression, anxiety and perceived social support in subfertile women who conceived after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatment. METHODS: A prospective cohort study was developed. Data were collected on subfertile patients who conceived after IVF/ICSI treatment. All demographic and clinical data were recorded...
February 21, 2017: Journal of Psychosomatic Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28635527/whose-fault-is-it-blame-predicting-psychological-adjustment-and-couple-satisfaction-in-couples-seeking-fertility-treatment
#17
Katherine Péloquin, Audrey Brassard, Virginie Arpin, Stéphane Sabourin, John Wright
Infertility bears psychological and relational consequences for couples who face this problem. Few studies have examined the role of self- and partner blaming to explain psychological and relationship adjustment in couple presenting with a fertility problem. This study used a dyadic approach to explore the links between blaming oneself and one's partner and both partners' symptoms of depression and anxiety, and couple satisfaction in 279 couples enrolled in fertility treatments. Partners were questioned about the extent to which they blamed themselves and their partner for the fertility problem...
February 9, 2017: Journal of Psychosomatic Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28635411/single-nucleotide-polymorphisms-in-the-human-rad21l-gene-may-be-a-genetic-risk-factor-for-japanese-patients-with-azoospermia-caused-by-meiotic-arrest-and-sertoli-cell-only-syndrome
#18
Gaku Minase, Toshinobu Miyamoto, Yasushi Miyagawa, Masashi Iijima, Hiroto Ueda, Yasuaki Saijo, Mikio Namiki, Kazuo Sengoku
Genetic mechanisms are implicated in some cases of male infertility. Recently, it was demonstrated that male mice lacking the gene for RAD21L exhibited azoospermia caused by meiotic arrest. Mouse RAD21L is a functionally relevant meiotic α-kleisin that is essential for male fertility. Therefore, we hypothesized that RAD21L mutations or polymorphisms may be associated with male infertility, especially azoospermia secondary to meiotic arrest. To determine if RAD21L defects are associated with azoospermia in groups of patients with meiotic arrest, we performed direct sequencing of the RAD21L coding regions in 38 Japanese patients with meiotic arrest and in 200 normal controls...
February 21, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28635410/pelvic-floor-dysfunction-and-polycystic-ovary-syndrome
#19
Seyed Abdolvahab Taghavi, Fatemeh Bazarganipour, Helen Allan, Zahra Khashavi, Nasrin Reisi, Nadiyeh Dosha, Fatemeh Aghili, Mohadeseh Keramati, Sedigheh Zahedi, Amireh Aji-Ramkani
The purpose of this study was to compare the prevalence of pelvic floor dysfunction (PFD) symptoms in patients with and without polycystic ovary syndrome (PCOS) and among different PCOS phenotypes. This was a case-control study. All participants were assessed using ultrasonography to determine the presence or absence of polycystic ovaries (PCO) and allocated to the case or control group (having healthy females whose husbands were diagnosed with male infertility). The case group was then further subdivided into the three phenotypes according to the presence or absence of menstrual dysfunction (M), hyperandrogenism (HA) and PCO on ultrasonography...
February 21, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28635409/confronting-infertility-in-iranian-clients-a-grounded-theory
#20
Mitra Zandi, Eesa Mohammadi, Zohreh Vanaki, Marziyeh Shiva, Narges Bagheri Lankarani, Faezeh Zarei
The present study examined how infertile Iranian clients confront infertility. Research on infertility has confirmed the significance of a socio-cultural context in the formation of responses to infertility. This study used the grounded theory approach to explore the experiences of infertile individuals presenting at the Royan Institute in Iran from October 2013 to September 2014. Clients with infertility of at least two years in duration were recruited using purposive sampling followed by theoretical sampling...
February 10, 2017: Human Fertility: Journal of the British Fertility Society
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