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https://www.readbyqxmd.com/read/29773926/comparative-analysis-of-airborne-bacteria-and-fungi-in-two-salt-mines-in-poland
#1
Elżbieta Gębarowska, Wojciech Pusz, Jolanta Kucińska, Włodzimierz Kita
The aim of this work was to determine the genera or species composition and the number of colony forming units of airborne bacteria and fungi, respectively, in two salt mines in Poland "Wieliczka" (Lesser Poland) and "Polkowice-Sieroszowice" (Lower Silesia). Both of them are working environments characterized by extreme conditions, and additionally "Wieliczka," officially placed on the UNESCO World Heritage Sites' list, plays a role of tourist attraction. There are also some curative chambers located in this mine...
2018: Aerobiologia
https://www.readbyqxmd.com/read/29758310/chemical-synthesis-and-characterization-of-chitosan-silver-nanocomposites-films-and-their-potential-antibacterial-activity
#2
Aamna Shah, Izhar Hussain, Ghulam Murtaza
This study provides the optimum preparation parameters for functional chitosan silver nanocomposite (CSN) films with promising antibacterial efficacy though prepared with very low silver nitrate (AgNO3 ) concentration. Chitosan nano‑silver composites were fabricated by In-situ chemical method utilizing the reducing ability of sodium borohydride (NaBH4 ) and afterward casted into films. Utilization of response surface methodology, NCSS, and SigmaPlot for the optimization of CSN and their predicted antibacterial efficacy assessment of the selected bacterial strains (standard and clinical) was the essential part of the study...
May 11, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29742926/iclaprim-a-dihydrofolate-reductase-inhibitor-antibiotic-in-phase-iii-of-clinical-development-a-review-of-its-pharmacology-microbiology-and-clinical-efficacy-and-safety
#3
David B Huang, Matthew Dryden
Iclaprim is under clinical development for treating acute bacterial skin and skin structure infections (ABSSSI) and nosocomial pneumonia most often due to Gram-positive bacteria, including infections due to drug-resistant bacteria. In two recent Phase III studies of patients with acute bacterial skin and skin structure infections, intravenous iclaprim 80 mg every 12 h was noninferior to dose-adjusted vancomycin. Additional studies are planned for patients with nosocomial pneumonia. Iclaprim represents an alternative for the treatment of severe skin and pulmonary infections due to Gram-positive bacteria...
May 10, 2018: Future Microbiology
https://www.readbyqxmd.com/read/29734776/an-ointment-consisting-of-the-phage-lysin-lysgh15-and-apigenin-for-decolonization-of-methicillin-resistant-staphylococcus-aureus-from-skin-wounds
#4
Mengjun Cheng, Lei Zhang, Hao Zhang, Xinwei Li, Yanmei Wang, Feifei Xia, Bin Wang, Ruopeng Cai, Zhimin Guo, Yufeng Zhang, Yalu Ji, Changjiang Sun, Xin Feng, Liancheng Lei, Yongjun Yang, Wenyu Han, Jingmin Gu
Staphylococcus aureus ( S. aureus ) is a common and dangerous pathogen that causes various infectious diseases. Skin damage, such as burn wounds, are at high risk of Staphylococcus aureus colonization and infection, which increases morbidity and mortality. The phage lysin LysGH15 exhibits highly efficient lytic activity against methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains. Apigenin (api) significantly decreases haemolysis of rabbit erythrocytes caused by S. aureus and shows anti-inflammatory function...
May 6, 2018: Viruses
https://www.readbyqxmd.com/read/29725514/actinomycosis-of-distal-phalanx-twenty-years-after-flap-reconstruction-of-index-finger-a-case-report
#5
N Prashant, A Azuhairy
Actinomycosis is a chronic granulomatous suppurative infection caused by anaerobic bacteria from genus Actinomyces which are normal flora of mouth, colon and vagina. Actinomycosis of upper extremity is rare. We report a case of actinomycosis of the distal phalanx of finger many years after flap reconstruction. The patient presented with two months' history of chronic discharging sinus from the tip of his right index finger, which had sustained a degloving injury 20 years previously. It had been treated with an anterior chest wall flap which had healed uneventfully but was bulky due to excess tissue from the donor site...
March 2018: Malaysian Orthopaedic Journal
https://www.readbyqxmd.com/read/29723373/skin-manifestations-of-tick-bites-in-humans
#6
Vidal Haddad, Michel Raineri Haddad, Mônica Santos, João Luiz Costa Cardoso
Ticks are blood-sucking arthropods that attach to human skin through oral devices causing diverse initial cutaneous manifestations, and may also transmit serious infectious diseases. In certain situations, the Health Teams (and especially dermatologists) may face difficulties in identifying the lesions and associating them to the parasites. To assist them in clinical diagnosis, we suggest a classification of the skin manifestations in primary lesions, which occur by the attachment the tick to the host (for toxicity and the anticoagulant substances in the saliva and/or marked inflammation by the penetration and permanence of the mouthparts) and secondary lesions that are manifestations of infections caused by rickettsia, bacteria, protozoa and fungi inoculated by the ticks...
March 2018: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/29720571/first-in-human-topical-microbiome-transplantation-with-roseomonas-mucosa-for-atopic-dermatitis
#7
Ian A Myles, Noah J Earland, Erik D Anderson, Ian N Moore, Mark D Kieh, Kelli W Williams, Arhum Saleem, Natalia M Fontecilla, Pamela A Welch, Dirk A Darnell, Lisa A Barnhart, Ashleigh A Sun, Gulbu Uzel, Sandip K Datta
The underlying pathology of atopic dermatitis (AD) includes impaired skin barrier function, susceptibility to Staphylococcus aureus skin infection, immune dysregulation, and cutaneous dysbiosis. Our recent investigation into the potential role of Gram-negative skin bacteria in AD revealed that isolates of one particular commensal, Roseomonas mucosa, collected from healthy volunteers (HVs) improved outcomes in mouse and cell culture models of AD. In contrast, isolates of R. mucosa from patients with AD worsened outcomes in these models...
May 3, 2018: JCI Insight
https://www.readbyqxmd.com/read/29713459/effect-of-pre-milking-teat-disinfection-on-new-mastitis-infection-rates-of-dairy-cows
#8
David Gleeson, Jimmy Flynn, Bernadette O' Brien
Background: The practise of teat disinfection prior to cluster attachment for milking is being adopted by farmers in Ireland, particularly where there are herd issues with new infection rates. Pre-milking teat disinfection has been shown to reduce bacterial numbers on teat skin and to be most effective against environmental bacteria such as Escherichia coli and Streptococcus uberis. A split udder design experiment was undertaken on two research herds (A = 96 cows: B = 168 cows) to test the benefit of pre-milking teat disinfection on new mastitis infection levels...
2018: Irish Veterinary Journal
https://www.readbyqxmd.com/read/29713166/in-vivo-antimicrobial-activity-of-silver-nanoparticles-produced-via-a-green-chemistry-synthesis-using-acacia-rigidula-as-a-reducing-and-capping-agent
#9
Carlos Enrique Escárcega-González, J A Garza-Cervantes, A Vázquez-Rodríguez, Liliana Zulem Montelongo-Peralta, M T Treviño-González, E Díaz Barriga Castro, E M Saucedo-Salazar, R M Chávez Morales, D I Regalado Soto, F M Treviño González, J L Carrazco Rosales, Rocío Villalobos Cruz, José Rubén Morones-Ramírez
Introduction: One of the main issues in the medical field and clinical practice is the development of novel and effective treatments against infections caused by antibiotic-resistant bacteria. One avenue that has been approached to develop effective antimicrobials is the use of silver nanoparticles (Ag-NPs), since they have been found to exhibit an efficient and wide spectrum of antimicrobial properties. Among the main drawbacks of using Ag-NPs are their potential cytotoxicity against eukaryotic cells and the latent environmental toxicity of their synthesis methods...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29709605/catalase-a-is-involved-in-the-response-to-photooxidative-stress-in-pseudomonas-aeruginosa
#10
Viviana Teresa Orlandi, Eleonora Martegani, Fabrizio Bolognese
BACKGROUND: Pseudomonas aeruginosa is the etiological agent of systemic and skin infections that are often difficult to treat. Photodynamic therapy (PDT) and, more recently, phototherapy (PT), are emerging among antimicrobial treatments to be combined with antibiotics. Visible light, either alone or combined with a photosensitizer (PS), elicits photooxidative stress that induces microbial death. The response of bacteria to phototherapy seems to involve the antioxidant machinery. This study relies on the effects of detoxifying catalase A (KatA) in response to PDT and PT-induced photooxidative stress...
April 27, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29709592/the-skin-immune-response-of-rainbow-trout-oncorhynchus-mykiss-walbaum-associated-with-puffy-skin-disease-psd
#11
Lyndsay Christie, Ronny van Aerle, Richard K Paley, David W Verner-Jeffreys, Hannah Tidbury, Matthew Green, Stephen W Feist, Irene Cano
Puffy skin disease (PSD) is an emerging skin condition which affects rainbow trout, Oncorhynchus mykiss (Walbaum). The transmission pattern of PSD suggests an infectious aetiology, however, the actual causative infectious agent(s) remain(s) unknown. In the present study, the rainbow trout epidermal immune response to PSD was characterised. Skin samples from infected fish were analysed and classified as mild, moderate or severe PSD by gross pathology and histological assessment. The level of expression of 26 immune-associated genes including cytokines, immunoglobulins and cell markers were examined by TaqMan qPCR assays...
April 27, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29704185/the-effect-of-heated-co-2-insufflation-in-minimising-surgical-wound-contamination-during-open-surgery
#12
Monika Baumann, John E Cater
The primary source of infections in open surgeries has been found to be bacteria and viruses carried into the surgical wound on the surfaces of skin particles shed by patients and surgical staff. In open cardiac surgeries, insufflation of the wound with carbon dioxide is used to limit the quantity of air able to enter into the heart, avoiding air embolisms when the heart is restarted. This surgical technique has been evaluated as a method of limiting the number of skin particles able to enter into the wound, using computational fluid dynamics (CFD) simulations and experimental testing...
April 27, 2018: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29696813/hyaluronan-based-nanohydrogels-for-targeting-intracellular-s-aureus-in-human-keratinocytes
#13
Elita Montanari, Angela Oates, Chiara Di Meo, Josephine Meade, Rugiada Cerrone, Antonio Francioso, Deirdre Devine, Tommasina Coviello, Patrizia Mancini, Luciana Mosca, Pietro Matricardi
Staphylococcus aureus is one of the most significant human pathogens that is frequently isolated in a wide range of superficial and systemic infections. The ability of S. aureus to invade and survive within host cells such as keratinocytes and host immune cells has been increasingly recognized as a potential factor in persistent infections and treatment failures. The incorporation of antibiotics into hyaluronan-cholesterol nanohydrogels represents a novel paradigm in the delivery of therapeutic agents against intracellular bacteria...
April 25, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29695061/use-of-leukocyte-platelet-l-prf-rich-fibrin-in-diabetic-foot-ulcer-with-osteomyelitis-three-clinical-cases-report
#14
Alessandro Crisci, Giuseppe Marotta, Anna Licito, Edda Serra, Giulio Benincasa, Michela Crisci
In this study, the use of fibrin rich in leukocytes and platelets (L-PRF) was explored to heal osteomyelitis ulcers in a diabetic foot. The goal was to standardize the utilization of L-PRF in patients with osteomyelitis to direct it for healing. L-PRF was obtained autologously from the peripheral blood of the diabetic patients ( n = 3) having osteomyelitis and skin lesions for at least six months. The L-PRF and supernatant serum were inserted into the skin lesion to the bone after a surgical debridement. The evolution of lesions over time was analyzed...
April 24, 2018: Diseases (Basel)
https://www.readbyqxmd.com/read/29693635/commensal-staphylococcus-aureus-provokes-immunity-to-protect-against-skin-infection-of-methicillin-resistant-staphylococcus-aureus
#15
John-Jackson Yang, Ting-Wei Chang, Yong Jiang, Hsin-Jou Kao, Bin-Hao Chiou, Ming-Shan Kao, Chun-Ming Huang
Unlike USA300, a strain of community-acquired methicillin-resistant Staphylococcus aureus (MRSA), commensal Staphylococcus aureus ( S. aureus ) bacteria isolated from human skin demonstrated the ability to mediate the glycerol fermentation to produce short-chain fatty acids (SCFAs). Quantitative proteomic analysis of enzymes involved in glycerol fermentation demonstrated that the expression levels of six enzymes, including glycerol-3-phosphate dehydrogenase (GPDH) and phosphoglycerate mutase (PGM), in commensal S...
April 25, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29693373/fabrication-of-self-healing-hydrogel-with-on-demand-antimicrobial-activity-and-sustained-biomolecules-release-for-infected-skin-regeneration
#16
Ran Tian, Xinyu Qiu, Pingyun Yuan, Kai Lei, Lin Wang, Yongkang Bai, Shiyu Liu, Xin Chen
Microbial infection has been considered as one of the most critical challenges in bioengineering applications especially in tissue regeneration, which engender severe threat to public health. Herein, a hydrogel performing properties of rapid self-healing, on-demand antibiosis and controlled cargo release were fabricated by a simple assembly of Fe complex as crosslinker and hyaluronic acid as gel network. This hydrogel is able to locally degrade and release Fe3+ to kill bacteria as needed, due to the hyaluronidase excreted by surrounding bacteria, resulting in efficient antibacterial activity to different types of bacteria...
April 25, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29690741/-antiseptic-effect-of-compound-lysostaphin-disinfectant-and-its-preventive-effect-on-infection-of-artificial-dermis-after-graft-on-full-thickness-skin-defect-wound-in-rats
#17
J Jin, H Zhou, Z C Cui, L Wang, P F Luo, S Z Ji, X Y Hu, B Ma, G Y Wang, S H Zhu, Z F Xia
Objective: To study the antiseptic effect of compound lysostaphin disinfectant and its preventive effect on infection of artificial dermis after graft on full-thickness skin defect wound in rats. Methods: (1) Each one standard strain of Klebsiella pneumoniae, Acinetobacter baumannii, and Staphylococcus aureus were selected. Each 20 clinical strains of Klebsiella pneumoniae, Acinetobacter baumannii, and Staphylococcus aureus were collected from those isolated from wound exudates of burn patients hospitalized in our wards from January 2014 to December 2016 according to the random number table...
April 20, 2018: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/29683289/antimicrobial-effects-of-photodynamic-therapy-an-overview
#18
Vanesa Pérez-Laguna, Ana J García-Malinis, Carmen Aspiroz, Antonio Rezusta, Yolanda Gilaberte
The microorganisms that cause infections are increasing their resistance to antibiotics. In this context, alternative treatments are necessary. The antimicrobial photodynamic therapy (aPDT) is a therapeutic modality based on photosensitizing molecules that end up generating reactive oxygen species that induce the destruction of the target cells when are irradiated with light of a suitable wavelength and at a proper dose. The cells targeted by aPDT are all types of microorganisms (bacteria, fungi and parasites) including viruses and has been proven effective against representative members of all of them...
April 19, 2018: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/29675414/spondyloarthritis-acute-anterior-uveitis-and-fungi-updating-the-catterall-king-hypothesis
#19
Martin Laurence, Mark Asquith, James T Rosenbaum
Spondyloarthritis is a common type of arthritis which affects mostly adults. It consists of idiopathic chronic inflammation of the spine, joints, eyes, skin, gut, and prostate. Inflammation is often asymptomatic, especially in the gut and prostate. The HLA-B*27 allele group, which presents intracellular peptides to CD8+ T cells, is by far the strongest risk factor for spondyloarthritis. The precise mechanisms and antigens remain unknown. In 1959, Catterall and King advanced a novel hypothesis explaining the etiology of spondyloarthritis: an as-yet-unrecognized sexually acquired microbe would be causing all spondyloarthritis types, including acute anterior uveitis...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29673880/french-hospital-discharge-database-pmsi-and-bacterial-resistance-is-coding-adapted-to-hospital-epidemiology
#20
L de Léotoing, F Barbier, A Dinh, D Breilh, G Chaize, A Vainchtock, L Levy-Bachelot, C Bensoussan, S Dramard, J Fernandes
OBJECTIVE: A preliminary analysis of data consistency on different types of bacterial resistance by infection site and causative agents was conducted using the French hospital discharge database (French acronym PMSI) to assess the use of the database in a national cartography tool. MATERIAL AND METHODS: Hospital stays in medical, surgical, and obstetrical units were extracted from the 2014 PMSI database using the ICD-10 diagnosis codes. Bacterial infections, causative agents, and resistance corresponding to these stays were also identified...
April 17, 2018: Médecine et Maladies Infectieuses
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