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https://www.readbyqxmd.com/read/28934124/n-halamine-biocidal-materials-with-superior-antimicrobial-efficacies-for-wound-dressings
#1
Buket Demir, Roy M Broughton, Mingyu Qiao, Tung-Shi Huang, S D Worley
This work demonstrated the successful application of N-halamine technology for wound dressings rendered antimicrobial by facile and inexpensive processes. Four N-halamine compounds, which possess different functional groups and chemistry, were synthesized. The N-halamine compounds, which contained oxidative chlorine, the source of antimicrobial activity, were impregnated into or coated onto standard non-antimicrobial wound dressings. N-halamine-employed wound dressings inactivated about 6 to 7 logs of Staphylococcus aureus and Pseudomonas aeruginosa bacteria in brief periods of contact time...
September 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28932812/comparison-of-the-microbiota-of-older-adults-living-in-nursing-homes-and-the-community
#2
Mary-Claire Roghmann, Alison D Lydecker, Lauren Hittle, Robert T DeBoy, Rebecca G Nowak, J Kristie Johnson, Emmanuel F Mongodin
Our objective for this study was to characterize the microbial communities of the anterior nares (nose), posterior pharynx (throat), and skin of the femoral and subclavian areas in older adults from nursing homes and the community. Older adults (≥65 years) without antibiotic use for the past 3 months were recruited from nursing homes (NH; n = 16) and from the community (CB; n = 51). Specimens were taken from nose, throat, and skin sites for culture and bacterial profiling using 16S rRNA gene sequencing...
September 2017: MSphere
https://www.readbyqxmd.com/read/28927409/biological-and-physicochemical-properties-of-biosurfactants-produced-by-lactobacillus-jensenii-p6a-and-lactobacillus-gasseri-p65
#3
I M C Morais, A L Cordeiro, G S Teixeira, V S Domingues, R M D Nardi, A S Monteiro, R J Alves, E P Siqueira, V L Santos
BACKGROUND: Lactobacillus species produce biosurfactants that can contribute to the bacteria's ability to prevent microbial infections associated with urogenital and gastrointestinal tracts and the skin. Here, we described the biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65. RESULTS: The biosurfactants produced by L. jensenii P6A and L. gasseri P65 reduced the water surface tension from 72 to 43...
September 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28912020/intestinal-dysbiosis-featuring-abundance-of-ruminococcus-gnavus-associates-with-allergic-diseases-in-infants
#4
Huey-Huey Chua, Hung-Chieh Chou, Ya-Ling Tung, Bor-Luen Chiang, Chien-Chia Liao, Hong-Hsing Liu, Yen-Hsuan Ni
BACKGROUND & AIMS: Dysbiosis of the intestinal microbiota has been associated with development of allergies in infants. However, it is not clear what microbes might contribute to this process. We investigated what microbe(s) might be involved in analyses of infant twins and mice. METHODS: We studied fecal specimens prospectively in a twin cohort (n=30) and age-matched singletons (n=14) born at National Taiwan University Children's Hospital, Taipei, Taiwan, from April 2011 to March 2013...
September 11, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28911341/rapid-detection-of-staphylococcus-aureus-and-related-species-isolated-from-food-environment-cosmetics-a-medical-device-and-clinical-samples-using-the-vitek-ms-microbial-identification-system
#5
Irshad M Sulaiman, Pratik Banerjee, Ying-Hsin Hsieh, Nancy Miranda, Steven Simpson, Khalil Kerdahi
Staphylococcus spp. is considered as one of the most common human-pathogenic bacteria, causing illnesses ranging from nonthreatening skin infections to lethal diseases, including sepsis, pneumonia, bloodstream infections, and food poisoning. The emergence of methicillin-resistant Staphylococcus aureus strains has increased morbidity and mortality and resulted in a major healthcare burden worldwide. Single and multilocus sequence typing have been extensively used in the identification of Staphylococcus species...
September 15, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28887976/nanoclay-cross-linked-semi-ipn-silk-sericin-poly-nipam-lmsh-nanocomposite-hydrogel-an-outstanding-antibacterial-wound-dressing
#6
Chaochao Yang, Rui Xue, Qingsong Zhang, Shulin Yang, Pengfei Liu, Li Chen, Ke Wang, Xiaoyong Zhang, Yen Wei
High antibacterial and skin-like hydrogels have always been the perfect wound dressing for human to protect wound from infection. Here, based on silk sericin, we design a series of nanoclay lithium magnesium silicate hydrate (LMSH) cross-linked semi-IPN sericin/poly(NIPAm/LMSH) (HSP) nanocomposite hydrogels and demonstrate advantages in serving as antibacterial wound dressing in comparison with gauze. Firstly, the effect of mass ratios of sericin/(sericin+NIPAm) upon pore structure, feasibility of mechanics and gas permeability of HSP nanocomposite hydrogels were evaluated...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28885829/membrane-active-amphipathic-peptide-wrl3-with-in-vitro-antibiofilmcapability-and-in-vivo-efficacy-in-treating-mrsa-burn-wound-infections
#7
Zhi Ma, Jinzhi Han, Bingxue Chang, Ling Gao, Zhaoxin Lu, Fengxia Lu, Haizhen Zhao, Chong Zhang, Xiaomei Bie
Methicillin-resistant Staphylococcus aureus (MRSA) has become increasingly prevalent in hospitals, clinics, and the community. MRSA can cause significant and even lethal infections, especially in skin burn wounds. The currently available topical agents have largely failed to eliminate MRSA infections due to resistance. Therefore, there is an urgent need for new and effective approaches for treating MRSA. Here, we show that a novel engineered amphipathic peptide, WRL3, exhibits potent antimicrobial activity against MRSA, even in the presence of various salts or serum...
September 8, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28885820/strategies-for-editing-virulent-staphylococcal-phages-using-crispr-cas10
#8
S M Nayeemul Bari, Forrest C Walker, Katie Cater, Barbaros Aslan, Asma Hatoum-Aslan
Staphylococci are prevalent skin-dwelling bacteria that are also leading causes of antibiotic-resistant infections. Viruses that infect and lyse these organisms (virulent staphylococcal phages) can be used as alternatives to conventional antibiotics and represent promising tools to eliminate or manipulate specific species in the microbiome. However, since over half their genes have unknown functions, virulent staphylococcal phages carry inherent risk to cause unknown downstream side effects. Further, their swift and destructive reproductive cycle make them intractable by current genetic engineering techniques...
September 21, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28883811/culture-media-and-individual-hosts-affect-the-recovery-of-culturable-bacterial-diversity-from-amphibian-skin
#9
Daniel Medina, Jenifer B Walke, Zachary Gajewski, Matthew H Becker, Meredith C Swartwout, Lisa K Belden
One current challenge in microbial ecology is elucidating the functional roles of the large diversity of free-living and host-associated bacteria identified by culture-independent molecular methods. Importantly, the characterization of this immense bacterial diversity will likely require merging data from culture-independent approaches with work on bacterial isolates in culture. Amphibian skin bacterial communities have become a recent focus of work in host-associated microbial systems due to the potential role of these skin bacteria in host defense against the pathogenic fungus Batrachochytrium dendrobatidis (Bd), which is associated with global amphibian population declines and extinctions...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28879609/-bacterial-skin-and-soft-tissue-infections
#10
REVIEW
Frank Hanses
Bacterial skin and soft tissue infections are frequent and represent one of the most important differential diagnoses in patients presenting with reddened and swollen skin and soft tissue. Among these infections, cellulitis (phlegmon), erysipelas and abscesses are the most frequent. Whereas erysipelas is almost exclusively caused by streptococci and can be successfully treated with penicillin, an effective antibiotic therapy targeted at Staphylococcus aureus should be empirically considered for abscesses and phlegmon; other pathogens, such as gram negative and anaerobic bacteria can be found in patients with the corresponding underlying disease and certain risk factors...
September 6, 2017: Zeitschrift Für Rheumatologie
https://www.readbyqxmd.com/read/28875068/skin-bacterial-microbiome-of-a-generalist-puerto-rican-frog-varies-along-elevation-and-land-use-gradients
#11
Myra C Hughey, Janelle A Pena, Roberto Reyes, Daniel Medina, Lisa K Belden, Patricia A Burrowes
Host-associated microbial communities are ubiquitous among animals, and serve important functions. For example, the bacterial skin microbiome of amphibians can play a role in preventing or reducing infection by the amphibian chytrid fungus, Batrachochytrium dendrobatidis. Evidence suggests that environmental bacteria likely serve as a source pool for at least some of the members of the amphibian skin bacterial community, underscoring the potential for local environmental changes to disrupt microbial community source pools that could be critical to the health of host organisms...
2017: PeerJ
https://www.readbyqxmd.com/read/28873062/a-prospective-randomized-controlled-clinical-trial-to-assess-use-of-2-lidocaine-irrigation-to-prevent-abdominal-surgical-site-infection
#12
Alejandro Quiroga-Garza, Juan Manuel Valdivia-Balderas, Miguel Ángel Trejo-Sánchez, Abraham Guadalupe Espinosa-Uribe, Cynthia Guadalupe Reyes-Hernández, Rodrigo Enrique Elizondo-Omaña
Surgical site infections (SSI) are the third most common nosocomial infection, increasing morbidity and mortality rates of patients as well as their costs of care, but longer-term follow up studies and data are limited. Lidocaine, in addition to being a readily available and accessible local anesthetic, is known to have bacteriostatic properties. A prospective, descriptive, controlled, randomized clinical trial was conducted among patients scheduled to undergo abdominal sur- gery in the general surgical unit of a Mexican hospital...
August 2017: Ostomy/wound Management
https://www.readbyqxmd.com/read/28865506/-anaerobic-bacteria-with-clinical-relevance-morphologic-and-taxonomic-classification-distribution-among-human-microbiota-and-microbiologic-diagnosis
#13
João Alves, Susana Peres, Elsa Gonçalves, Kamal Mansinho
The wide burden of anaerobic bacteria colonizing human body comprises about 90% of its total biomass. The biotic relationship between humans and its microbiota sets reciprocal benefits, albeit with pathogenic potencial for the human being in particular dysbiosis situations. Infections adjacent to or originating from the skin or mucous membranes of the intestinal, genitourinary and upper respiratory tracts are often polymicrobial in nature, whereby should anaerobes be invariably included in the etiological differential diagnosis of these conditions...
May 31, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28864654/exogenous-polyunsaturated-fatty-acids-pufas-impact-membrane-remodeling-and-affect-virulence-phenotypes-among-pathogenic-vibrio-species
#14
Anna R Moravec, Andrew W Siv, Chelsea R Hobby, Emily N Lindsay, Layla V Norbash, Daniel J Shults, Steven J K Symes, David K Giles
The pathogenic Vibrio species (cholerae, parahaemolyticus and vulnificus) represent a constant threat to human health, causing food-borne and skin wound infections as a result of ingestion or exposure to contaminated water and seafood. Recent studies have highlighted Vibrio's ability to acquire fatty acids from environmental sources and assimilate them into cell membranes. The possession and conservation of such machinery provokes consideration of fatty acids as important factors in the pathogenic lifestyle of Vibrio species...
September 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28860757/photodynamic-antimicrobial-chemotherapy-for-staphylococcus-aureus-and-multidrug-resistant-bacterial-burn-infection-in-vitro-and-in-vivo
#15
Bingjie Mai, Yiru Gao, Min Li, Xiaobing Wang, Kun Zhang, Quanhong Liu, Chuanshan Xu, Pan Wang
BACKGROUND AND OBJECTIVES: Antibiotic resistance has emerged as one of the most important determinants of outcome in patients with serious infections, along with the virulence of the underlying pathogen. Photodynamic antimicrobial chemotherapy (PACT) has been proposed as an alternative approach for the inactivation of bacteria. This study aims to evaluate the antibacterial effect of sinoporphyrin sodium (DVDMS)-mediated PACT on Staphylococcus aureus and multidrug resistant S. aureus in vitro and in vivo...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28844382/antibacterial-effect-and-proposed-mechanism-of-action-of-a-topical-surgical-adhesive
#16
Daniel Prince, Zankhna Solanki, Remy Varughese, Jozef Mastej, Derek Prince
BACKGROUND: Medical adhesives effectively hold closed approximated skin edges of wounds from surgical incisions, including punctures from minimally invasive surgery. In addition, they have been reported to be antibacterial against gram-positive bacteria. METHODS: Using membrane filtration to capture all organisms after contact with 2-octyl cyanoacrylate product for 3 minutes, we quantified the number of survivors. Controls were performed to rule out that the noted level of kill was caused by carryover product in the test system...
August 22, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28842374/immobilization-of-bacteriophage-in-wound-dressing-nanostructure
#17
Frederico Nogueira, Natia Karumidze, Ia Kusradze, Marina Goderdzishvili, Pilar Teixeira, Isabel C Gouveia
Opportunistic bacteria that cause life-threatening infections are still a central problem associated with a healthcare setting. Bacteriophage capsid immobilization on nanostructured polymers maximizes its tail exposure and looks promising in applications toward skin-infections as alternative to antibiotics standardly used. The main goal of this work was to investigate the covalent immobilization of vB_Pae_Kakheti25 bacteriophage capsid on polycaprolactone (PCL) nanofibers (non-woven textile), as a potential effective antimicrobial, laundry resistant and non-toxic dressing for biomedical use...
August 25, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28837757/systemic-antibiotics-for-treating-malignant-wounds
#18
REVIEW
Darshini A Ramasubbu, Valerie Smith, Fiona Hayden, Patricia Cronin
BACKGROUND: Malignant wounds are a devastating complication of cancer. They usually develop in the last six months of life, in the breast, chest wall or head and neck regions. They are very difficult to treat successfully, and the commonly associated symptoms of pain, exudate, malodour, and the risk of haemorrhage are extremely distressing for those with advanced cancer. Treatment and care of malignant wounds is primarily palliative, and focuses on alleviating pain, controlling infection and odour from the wound, managing exudate and protecting the surrounding skin from further deterioration...
August 24, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28835801/synthetic-antibacterial-peptide-exhibits-synergy-with-oxacillin-against-mrsa
#19
John C Lainson, Seth M Daly, Kathleen Triplett, Stephen Albert Johnston, Pamela R Hall, Chris W Diehnelt
One proposed solution to the crisis of antimicrobial resistant (AMR) infections is the development of molecules that potentiate the activity of antibiotics for AMR bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA). Rather than develop broad spectrum compounds, we developed a peptide that could potentiate the activity of a narrow spectrum antibiotic, oxacillin. In this way, the combination treatment could narrowly target the resistant pathogen and limit impact on host flora. We developed a peptide, ASU014, composed of a S...
August 10, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28835551/land-cover-and-forest-connectivity-alter-the-interactions-among-host-pathogen-and-skin-microbiome
#20
C G Becker, A V Longo, C F B Haddad, K R Zamudio
Deforestation has detrimental consequences on biodiversity, affecting species interactions at multiple scales. The associations among vertebrates, pathogens and their commensal/symbiotic microbial communities (i.e. microbiomes) have important downstream effects for biodiversity conservation, yet we know little about how deforestation contributes to changes in host microbial diversity and pathogen abundance. Here, we tested the effects of landcover, forest connectivity and infection by the chytrid fungus Batrachochytrium dendrobatidis (Bd) on amphibian skin bacterial diversity along deforestation gradients in Brazilian landscapes...
August 30, 2017: Proceedings. Biological Sciences
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