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https://www.readbyqxmd.com/read/29775925/exposure-to-tris-1-3-dichloro-2-propyl-phosphate-for-two-generations-decreases-fecundity-of-zebrafish-at-environmentally-relevant-concentrations
#1
Yongkang Zhang, Meng Li, Shuying Li, Qiangwei Wang, Guonian Zhu, Guanyong Su, Robert J Letcher, Chunsheng Liu
Previous studies reported that exposure to environmentally relevant concentrations of TDCIPP significantly decreased the number of cumulative eggs in zebrafish, but effects on the quantity of eggs and sperms remained unknown. Therefore, in this study, effects of TDCIPP on yolk diameter, surface morphology of eggs, sperm density and total motility were evaluated. First generation (F0) zebrafish larvae (Danio rerio) were exposed to 0, 50, 500 or 5000 ng/L tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) from 14 days post fertilization (dpf) to 120 dpf...
May 14, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29775756/pkm2-a-potential-target-for-regulating-cancer
#2
REVIEW
Yu-Huan Li, Xiao-Feng Li, Jia-Tao Liu, Hua Wang, Lu-Lu Fan, Jun Li, Guo-Ping Sun
Aberrated glucose metabolism is a key future of cancer cells. Unlike normal cells, tumor cells favor glycolysis even in the presence of sufficient oxygen. Pyruvate kinase (PK), a key glucose metabolic enzyme, converts phosphoenolpyruvate (PEP) to pyruvate by transferring the high-energy phosphate group to adenosine diphosphate (ADP) to produce adenosine triphosphate (ATP). Pyruvate kinase M2 (PKM2), one of the four isozyme of PK, which universally expressed in rapidly proliferating cells such as embryonic cells and cancer cells...
May 15, 2018: Gene
https://www.readbyqxmd.com/read/29775097/phosphate-solubilizing-bacteria-improve-the-phytoremediation-efficiency-of-wedelia-trilobata-for-cu-contaminated-soil
#3
Maozi Lin, Meifang Jin, Kang Xu, Lingchao He, Dongliang Cheng
In a controlled experiment, we assessed the effect of phosphate-solubilizing bacterium (PSB) on the soil metal (Cu2+ ) phytoremediation by Wedelia trilobata and examined the effect of the interaction of Cu contamination and PSB on the growth of W. trilobata. We also explored the effect of the interaction of Cu contamination and PSB on the soil microflora. The results showed that the removal efficiency of Cu from soil by W. trilobata increased with an increase in the concentration of PSB, and the translocation factors of Cu (i...
July 3, 2018: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/29774984/comments-on-recent-reports-on-infrared-spectral-detection-of-disease-markers-in-blood-components
#4
Max Diem
The search for disease markers in whole blood, or easily accessible blood components by spectral methods is a highly important aspect in the field of biophotonic research for disease diagnostics and screening, since it promises a minimally invasive approach to assess an individual's state of health. Fourier transform infrared spectroscopy, in particular, promises to be a fast, inexpensive method to search for markers of disease, since it detects variation in the proteome, lipidome and metabolome of biofluids, or activation of immune cells...
May 18, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29774402/subtrochanteric-and-diaphyseal-femoral-fractures-in-hypophosphatasia-not-atypical-at-all
#5
F Genest, L Seefried
Risk for subtrochanteric and diaphyseal femoral fractures is considered increased in patients with hypophosphatasia (HPP). Evaluating a large cohort of HPP patients, we could for the first time quantify the prevalcence and identify both morphometric features as well as predisposing factors for this complication of severe HPP. INTRODUCTION: Subtrochanteric and diaphyseal femoral fractures have been associated with both, long-term antiresorptive treatment and metabolic bone disorders, specifically Hypophosphatasia (HPP)...
May 17, 2018: Osteoporosis International
https://www.readbyqxmd.com/read/29774288/molecular-analysis-of-giardia-duodenalis-isolates-from-symptomatic-and-asymptomatic-children-from-la-habana-cuba
#6
Luis Enrique Jerez Puebla, Fidel A Núñez, Lissette Pérez Santos, Lázara Rojas Rivero, Isabel Martínez Silva, Lucía Ayllón Valdés, Iraís Atencio Millán, Norbert Müller
Giardiasis is considered the most common intestinal parasitic disease in humans worldwide. In Cuba, this infection has particularly a strong clinical impact on the child population. Giardia duodenalis is a highly diverse protozoan, which comprises a complex of eight morphologically identical genetic assemblages, further divided into sub-assemblages. The present study used triose phosphate isomerase ( tpi ) and small-subunit ribosomal RNA (SSU rRNA) genes as genetic markers for the identification of G. duodenalis assemblages and sub-assemblages in correlation with clinical and epidemiological data in children attended at the Paediatric Hospital "William Soler" and at Pedro Kouri Institute, between 2015 and 2016...
August 2017: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29774240/mannan-induced-nos2-in-macrophages-enhances-il-17-driven-psoriatic-arthritis-by-innate-lymphocytes
#7
Jianghong Zhong, Tatjana Scholz, Anthony C Y Yau, Simon Guerard, Ulrike Hüffmeier, Harald Burkhardt, Rikard Holmdahl
Previous identification of the inducible nitric oxide synthase (NOS2) gene as a risk allele for psoriasis (Ps) and psoriatic arthritis (PsA) suggests a possible pathogenic role of nitric oxide (NO). Using a mouse model of mannan-induced Ps and PsA (MIP), where macrophages play a regulatory role by releasing reactive oxygen species (ROS), we found that NO was detectable before disease onset in mice, independent of a functional nicotinamide adenine dinucleotide phosphate oxidase 2 complex. MIP was suppressed by either deletion of Nos2 or inhibition of NO synthases with NG-nitro-l-arginine methyl ester, demonstrating that Nos2-derived NO is pathogenic...
May 2018: Science Advances
https://www.readbyqxmd.com/read/29774084/a-novel-dual-well-array-chip-for-efficiently-trapping-single-cell-in-large-isolated-micro-well-without-complicated-accessory-equipment
#8
Chenyu Wang, Wenwen Liu, Qingquan Wei, Lufeng Ren, Manqing Tan, Yude Yu
Conventional cell-sized well arrays have advantages of high occupancy, simple operation, and low cost for capturing single-cells. However, they have insufficient space for including reagents required for cell treatment or analysis, which restricts the wide application of cell-sized well arrays as a single-cell research tool alone. Here, we present a novel dual-well array chip, which integrates capture-wells (20  μ m in diameter) with reaction-wells (100  μ m in diameter) and describe a flow method for convenient single-cell analysis requiring neither complicated infra-structure nor high expenditure, while enabling highly efficient single cell trapping (75...
May 2018: Biomicrofluidics
https://www.readbyqxmd.com/read/29774080/microrna-193b-3p-regulates-chondrogenesis-and-chondrocyte-metabolism-by-targeting-hdac3
#9
Fangang Meng, Zhiwen Li, Zhiqi Zhang, Zibo Yang, Yan Kang, Xiaoyi Zhao, Dianbo Long, Shu Hu, Minghui Gu, Suiwen He, Peihui Wu, Zongkun Chang, Aishan He, Weiming Liao
Histone deacetylase 3 (HDAC3) plays a pivotal role in the repression of cartilage-specific gene expression in human chondrocytes. The aim of this study was to determine whether microRNA-193b-3p (miR-193b-3p) regulates the expression of HDAC3 during chondrogenesis and chondrocyte metabolism. Methods: miR-193b-3p expression was assessed in a human mesenchymal stem cell (hMSC) model of chondrogenesis, in interleukin-1β (IL-1β)-treated primary human chondrocytes (PHCs), and in non-degraded and degraded cartilage...
2018: Theranostics
https://www.readbyqxmd.com/read/29773802/cd4-t-cells-are-activated-in-regional-lymph-nodes-and-migrate-to-skin-to-initiate-lymphedema
#10
Gabriela D García Nores, Catherine L Ly, Daniel A Cuzzone, Raghu P Kataru, Geoffrey E Hespe, Jeremy S Torrisi, Jung Ju Huang, Jason C Gardenier, Ira L Savetsky, Matthew D Nitti, Jessie Z Yu, Sonia Rehal, Babak J Mehrara
T cell-mediated responses have been implicated in the development of fibrosis, impaired lymphangiogenesis, and lymphatic dysfunction in secondary lymphedema. Here we show that CD4+ T cells are necessary for lymphedema pathogenesis by utilizing adoptive transfer techniques in CD4 knockout mice that have undergone tail skin and lymphatic excision or popliteal lymph node dissection. We also demonstrate that T cell activation following lymphatic injury occurs in regional skin-draining lymph nodes after interaction with antigen-presenting cells such as dendritic cells...
May 17, 2018: Nature Communications
https://www.readbyqxmd.com/read/29773682/water-structure-changes-in-oxime-mediated-reactivation-process-of-phosphorylated-human-acetylcholinesterase
#11
Irina V Zueva, Sofya V Lushchekina, Patrick Masson
The role of water in oxime-mediated reactivation of phosphylated cholinesterases has been asked with recurrence. To investigate oximate water structure changes in this reaction, reactivation of paraoxon-inhibited human acetylcholinesterase (AChE) was performed by the oxime HI-6 at different pH in the presence and absence of lyotropic salts: a neutral salt (NaCl), a strong chaotropic salt (LiSCN) and strong kosmotropic salts (ammonium sulfate and phosphate HPO4 2- ). At the same time, molecular dynamic simulations of enzyme reactivation under the same conditions were performed over 100 ns...
May 17, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29773679/comparative-proteomic-analysis-reveals-the-regulatory-network-of-the-vea-gene-during-asexual-and-sexual-spore-development-of-aspergillus-cristatus
#12
Hui Liu, Shilei Sang, Hui Wang, Xiyi Ren, Yumei Tan, Wei Chen, Zuoyi Liu, Yongxiang Liu
Aspergillus cristatus belongs to the homothallic fungal group that undergoes a sexual stage without asexual conidiation under hypotonic conditions, while hypertonic medium induces initiation of the asexual stage and completely blocks sexual development. However, the veA deletion mutant only produces conidia in hypotonic medium after a 24 h culture, but both asexual and sexual spores are observed after 72 h. To elucidate the molecular mechanism of how VeA regulates asexual and sexual spore development in A. cristatus , two dimensional electrophoresis combined with matrix-assisted laser desorption/ionization-tandem time of flight mass spectrometry analysis were applied to identify 173 differentially expressed proteins (DEPs) by comparing the agamotype (24 h) and teleomorph (72 h) with wild-type A...
May 17, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29773673/lysosomal-proteome-and-secretome-analysis-identifies-missorted-enzymes-and-their-non-degraded-substrates-in-mucolipidosis-iii-mouse-cells
#13
Giorgia Di Lorenzo, Renata Voltolini Velho, Dominic Winter, Melanie Thelen, Shiva Ahmadi, Michaela Schweizer, Raffaella De Pace, Kerstin Cornils, Timur Alexander Yorgan, Saskia Grüb, Irm Hermans-Borgmeyer, Thorsten Schinke, Sven Müller-Loennies, Thomas Braulke, Sandra Pohl
Targeting of soluble lysosomal enzymes requires mannose 6-phosphate (M6P) signals whose formation is initiated by the hexameric N-acetylglucosamine (GlcNAc)-1-phosphotransferase complex (α2β2γ2). Upon proteolytic cleavage by site-1 protease, the α/β-subunit precursor is catalytically activated but the functions of γ-subunits (Gnptg) in M6P modification of lysosomal enzymes are unknown. To investigate this, we analyzed the Gnptg expression in mouse tissues, primary cultured cells, and in Gnptg reporter mice in vivo, and found high amounts in the brain, eye, kidney, femur, vertebra and fibroblasts...
May 17, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29773402/hydroxypropylmethyl-cellulose-hpmc-crosslinked-chitosan-ch-based-scaffolds-containing-bioactive-glass-bg-and-zinc-oxide-zno-for-alveolar-bone-repair
#14
Rabia Zeeshan, Zeeshan Mutahir, Haffsah Iqbal, Moazzam Ali, Farasat Iqbal, Kashif Ijaz, Faiza Sharif, Asma Tufail Shah, Aqif Anwar Chaudhry, Muhammad Yar, Shifang Luan, Ather Farooq Khan, Ihtesham-Ur-Rehman
The success of a dental implant relies on the presence of an optimal alveolar ridge. The aim of this study was to fabricate HPMC crosslinked chitosan based scaffolds for alveolar bone repair. Our results indicated that HPMC crosslinked CH/BG foams presented better morphological structure (132-90.5 μm) and mechanical responses (0.451 MPa with 100 mg BG) as confirmed by SEM analysis and fatigue testing respectively. Cytotoxicity analysis at day 2, 4 and 8 demonstrated that all composites were non-toxic and supported cellular viability...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29773401/optimization-of-orodispersible-and-conventional-tablets-using-simplex-lattice-design-relationship-among-excipients-and-banana-extract
#15
Sureewan Duangjit, Pakorn Kraisit
The objective of this study was focused on the optimization of the pharmaceutical excipients and banana extract in the preparation of orally disintegrating banana extract tablets (OD-BET) and conventional banana extract tablets (CO-BET) using a simplex lattice design. Various ratios of banana extract (BE), dibasic calcium phosphate (DCP) and microcrystalline cellulose (MCC) were used to prepare banana extract tablets (BET). The results indicated that the optimal OD-BET and CO-BET consisted of BE: DCP: MCC at 10...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29773373/environmentally-friendly-pathways-towards-the-synthesis-of-vinyl-based-oligocelluloses
#16
Azis Adharis, Dejan M Petrović, Ibrahim Özdamar, Albert J J Woortman, Katja Loos
The synthesis of vinyl-based oligocelluloses using cellodextrin phosphorylase as biocatalyst in buffer solution is presented. Various types of vinyl glucosides bearing (meth)acrylates/(meth)acrylamides functionalities served as the glucosyl acceptor in the enzyme catalyzed reverse phosphorolysis reaction and α-glucose 1-phosphate as the glucosyl donor. The enzymatic reaction was followed by thin layer chromatography and the isolated product yields were about 65%. The synthesized vinyl-based oligocelluloses had an average number of repeating glucosyl units and a number average molecular weight up to 8...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29773003/antioxidant-action-of-an-aqueous-extract-of-royal-sun-medicinal-mushroom-agaricus-brasiliensis-agaricomycetes-in-rats-with-adjuvant-induced-arthritis
#17
Aline Cristine da Silva de Souza, Geferson de Almeida Gonсalves, Andréia Assunсão Soares, Anacharis B de Sá-Nakanishi, Ana Paula de Santi-Rampazzo, Maria Raquel Marсal Natali, Rosane Marina Peralta, Adelar Bracht
The purpose of this study was to evaluate possible effects of the administration of an aqueous Agaricus brasiliensis extract on the oxidative state of the liver, brain, and plasma in adjuvant-induced arthritic rats, a model for human rheumatoid arthritis. Daily doses of 400 mg · kg-1 were administered by gavage for 23 days. This treatment produced significant diminutions in protein carbonylation and lipid damage in the liver, brain, and plasma. It also diminished the tissue reactive oxygen species and increased the antioxidant capacity of the plasma...
2018: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29772789/downregulation-of-the-s1p-transporter-spinster-homology-protein-2-spns2-exerts-an-anti-fibrotic-and-anti-inflammatory-effect-in-human-renal-proximal-tubular-epithelial-cells
#18
Olivier Blanchard, Bisera Stepanovska, Manuel Starck, Martin Erhardt, Isolde Römer, Dagmar Meyer Zu Heringdorf, Josef Pfeilschifter, Uwe Zangemeister-Wittke, Andrea Huwiler
Sphingosine kinase (SK) catalyses the formation of sphingosine 1-phosphate (S1P), which acts as a key regulator of inflammatory and fibrotic reactions, mainly via S1P receptor activation. Here, we show that in the human renal proximal tubular epithelial cell line HK2, the profibrotic mediator transforming growth factor β (TGFβ) induces SK-1 mRNA and protein expression, and in parallel, it also upregulates the expression of the fibrotic markers connective tissue growth factor (CTGF) and fibronectin. Stable downregulation of SK-1 by RNAi resulted in the increased expression of CTGF, suggesting a suppressive effect of SK-1-derived intracellular S1P in the fibrotic process, which is lost when SK-1 is downregulated...
May 17, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29772783/atpap2-a-unique-member-of-the-pap-family-functions-in-the-plasma-membrane
#19
Qingqing Sun, Jinyu Li, Wenzhen Cheng, Huihong Guo, Xiaomin Liu, Hongbo Gao
Purple acid phosphatases (PAPs) play various physiological roles in plants. AtPAP2 was previously shown to localize to both chloroplasts and mitochondria and to modulate carbon metabolism in Arabidopsis . Over-expression of AtPAP2 resulted in faster growth and increased biomass in several plant species, indicating its great potential for crop improvement of phosphate use and yield. Here, we studied the localization of AtPAP2 by transient expression in tobacco leaves. The results showed AtPAP2 was localized to the plasma membrane through the secretory pathway, which is different from previous studies...
May 17, 2018: Genes
https://www.readbyqxmd.com/read/29772449/recent-advances-in-the-synthesis-of-cyclic-5-nornucleoside-phosphonate-analogues
#20
Guang Huan Shen, Joon Hee Hong
Nucleoside phosphonates are isosteric, isopolar, and isoelectronic with phosphates. Nucleoside phosphonates can undergo enzymatic phosphorylation for conversion into the corresponding diphosphoryl phosphonates, which are naturally occurring nucleoside triphosphate analogues. The biological activity, which is mostly antiviral and antitumor but sometimes is as specific enzyme inhibitor, is briefly presented to help discover compounds with increased activity over natural nucleosides to provide structure-activity data...
April 28, 2018: Carbohydrate Research
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