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https://www.readbyqxmd.com/read/29430138/relative-presence-of-streptococcus-mutans-veillonella-atypica-and-granulicatella-adiacens-in-biofilm-of-complete-dentures
#1
Binoy Mathews Nedumgottil
Aims and Objective: Oral biofilms in denture wearers are populated with a large number of bacteria, a few of which have been associated with medical conditions such as sepsis and infective endocarditis (IE). The present study was designed to investigate the relative presence of pathogenic bacteria in biofilms of denture wearers specifically those that are associated with IE. Methods: Biofilm samples from 88 denture wearers were collected and processed to extract total genomic DNA...
January 2018: Journal of Indian Prosthodontic Society
https://www.readbyqxmd.com/read/29409543/effects-of-predation-stress-and-food-ration-on-perch-gut-microbiota
#2
Yinghua Zha, Alexander Eiler, Frank Johansson, Richard Svanbäck
BACKGROUND: Gut microbiota provide functions of importance to influence hosts' food digestion, metabolism, and protection against pathogens. Factors that affect the composition and functions of gut microbial communities are well studied in humans and other animals; however, we have limited knowledge of how natural food web factors such as stress from predators and food resource rations could affect hosts' gut microbiota and how it interacts with host sex. In this study, we designed a two-factorial experiment exposing perch (Perca fluviatilis) to a predator (pike, Esox lucius), and different food ratios, to examine the compositional and functional changes of perch gut microbiota based on 16S rRNA amplicon sequencing...
February 6, 2018: Microbiome
https://www.readbyqxmd.com/read/29385143/longitudinal-microbiome-analysis-of-single-donor-fecal-microbiota-transplantation-in-patients-with-recurrent-clostridium-difficile-infection-and-or-ulcerative-colitis
#3
Michael Mintz, Shanawaj Khair, Suman Grewal, Joseph F LaComb, Jiyhe Park, Breana Channer, Ramona Rajapakse, Juan Carlos Bucobo, Jonathan M Buscaglia, Farah Monzur, Anupama Chawla, Jie Yang, Charlie E Robertson, Daniel N Frank, Ellen Li
BACKGROUND: Studies of colonoscopic fecal microbiota transplant (FMT) in patients with recurrent CDI, indicate that this is a very effective treatment for preventing further relapses. In order to provide this service at Stony Brook University Hospital, we initiated an open-label prospective study of single colonoscopic FMT among patients with ≥ 2 recurrences of CDI, with the intention of monitoring microbial composition in the recipient before and after FMT, as compared with their respective donor...
2018: PloS One
https://www.readbyqxmd.com/read/29374490/apple-endophytic-microbiota-of-different-rootstock-scion-combinations-suggests-a-genotype-specific-influence
#4
Jia Liu, Ahmed Abdelfattah, John Norelli, Erik Burchard, Leonardo Schena, Samir Droby, Michael Wisniewski
BACKGROUND: High-throughput amplicon sequencing spanning conserved portions of microbial genomes (16s rRNA and ITS) was used in the present study to describe the endophytic microbiota associated with three apple varieties, "Royal Gala," "Golden Delicious," and "Honey Crisp," and two rootstocks, M.9 and M.M.111. The objectives were to (1) determine if the microbiota differs in different rootstocks and apple varieties and (2) determine if specific rootstock-scion combinations influence the microbiota composition of either component...
January 27, 2018: Microbiome
https://www.readbyqxmd.com/read/29365165/microbial-community-and-short-chain-fatty-acid-profile-in-gastrointestinal-tract-of-goose
#5
H Yang, Y Xiao, G Gui, J Li, J Wang, D Li
Goose is an economically important herbivore waterfowl supplying nutritious meat and eggs, high-quality liver fat, and feathers. However, biogeograhpy of the gut microbiome of goose remains limited. The aim of this study was to investigate the microbiota inhabiting 7 different gastrointestinal locations (proventriculus, gizzard, duodenum, jejunum, ileum, cecum, and rectum) of 180-day-old geese and the short-chain fatty acids (SCFA) of their metabolites based on 16S rRNA gene sequences and gas chromatography, respectively...
January 20, 2018: Poultry Science
https://www.readbyqxmd.com/read/29349504/oral-bacterial-colonization-on-dental-implants-restored-with-titanium-or-zirconia-abutments-6-month-follow-up
#6
Alice Ramos de Freitas, Thalisson Saymo de Oliveira Silva, Ricardo Faria Ribeiro, Rubens Ferreira de Albuquerque Junior, Vinícius Pedrazzi, Cássio do Nascimento
OBJECTIVE: This investigation aimed to characterize in a 6-month follow-up the microbial profile of implants restored with either titanium or zirconia abutments at the genus or higher taxonomic levels. METHODS: Twenty healthy individuals indicative for implant-retained single restorations were investigated. Half of participants were restored with titanium and half with zirconia abutments. Biofilm was collected from the implant-related sites after 1, 3, and 6 months of loading...
January 18, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29341956/microbiome-associated-with-denture-malodour
#7
S Yitzhaki, L Reshef, U Gophna, M Rosenberg, N Sterer
In the past, our inability to cultivate most of the oral microorganisms has limited our view of this complex ecosystem. In the present study, we utilized next generation deep sequencing techniques to revisit the microbiome associated with denture malodour, a growing field with the rise in life expectancy. The study population comprised 26 full dentures patients (mean age 71 ± 6.4, 10 males, 16 females) who visited the Tel Aviv University dental geriatric clinic. Denture malodour was rated organoleptically by a single odour judge, and dentures scoring 2 and above were considered malodour positive...
January 17, 2018: Journal of Breath Research
https://www.readbyqxmd.com/read/29306533/microbial-analysis-of-endodontic-infections-in-root-filled-teeth-with-apical-periodontitis-before-and-after-irrigation-using-pyrosequencing
#8
Homan Zandi, Anne K Kristoffersen, Dag Ørstavik, Isabela N Rôças, José F Siqueira, Morten Enersen
INTRODUCTION: The root canal microbiota in root-filled teeth with post-treatment apical periodontitis before and after chemomechanical instrumentation and irrigation with either 1% sodium hypochlorite (NaOCl) or 2% chlorhexidine digluconate were analyzed by using the pyrosequencing method. METHODS: Samples from 10 root-filled teeth with apical periodontitis undergoing retreatment were taken before (S1) and after (S2) preparation using irrigation with either NaOCl (n = 5) or 2% chlorhexidine digluconate (n = 5)...
January 3, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29287254/comparison-of-palygorskite-and-struvite-supported-palygorskite-derived-from-phosphate-recovery-in-wastewater-for-in-situ-immobilization-of-cu-pb-and-cd-in-contaminated-soil
#9
Hao Wang, Xuejiang Wang, Jing Li, Huanping Jing, Siqing Xia, Fuqiang Liu, Jianfu Zhao
A phosphorus-bearing product S-PAL obtained from nutrient-rich wastewater was reused as ameliorant for Cu, Pb and Cd immobilization in contaminated soil with three different rates (1%, 5% and 10% w/w). The 0.01 mol/L CaCl2 -extractability of metals significantly reduced with increasing rate of PAL and S-PAL in the first 7-day immobilization and insignificantly changed after 14-day immobilization. Compared with PAL, the lower metal extractability was observed after S-PAL addition. The BCR sequential extraction results showed that both of amendments were beneficial to transform acid soluble fraction to residual fraction...
December 17, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29284157/characterization-of-the-cervical-bacterial-community-in-dairy-cows-with-metritis-and-during-different-physiological-phases
#10
Yu Wang, Jifang Wang, Huatao Li, Kaiqiang Fu, Binbin Pang, Yan Yang, Yuan Liu, Wenru Tian, Rongfeng Cao
For the development of disease prevention and intervention strategies, a better understanding of the dynamics and interactions within cervical bacterial communities in both healthy cows and cows with metritis is required. Understanding the complexity and ecology of microorganisms in the vagina of dairy cows with metritis and during different physiological phases is critical for developing strategies to balance microorganism content. To gain deeper insight into fluctuations within the cervical microbiota, swab samples were collected from 40 Holstein dairy cows, and16S rDNA amplicon sequencing was used to analyze cervical bacterial diversity...
December 14, 2017: Theriogenology
https://www.readbyqxmd.com/read/29250055/the-microbiome-of-potentially-malignant-oral-leukoplakia-exhibits-enrichment-for-fusobacterium-leptotrichia-campylobacter-and-rothia-species
#11
Abdrazak Amer, Sheila Galvin, Claire M Healy, Gary P Moran
Oral leukoplakia presents as a white patch on the oral mucosa and is recognized as having significant malignant potential. Although colonization of these patches with Candida albicans is common, little is known about the bacterial microbiota of these patches. In the current study we analyzed the microbiome of oral leukoplakia in 36 patients compared to healthy mucosal tissue from the same patients and healthy control subjects to determine if specific microbial enrichments could be identified early in the malignant process that could play a role in the progression of the disease...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29234140/altered-gut-microbiota-profiles-in-sows-and-neonatal-piglets-associated-with-porcine-epidemic-diarrhea-virus-infection
#12
Deping Song, Qi Peng, Yanjun Chen, Xinrong Zhou, Fanfan Zhang, Anqi Li, Dongyan Huang, Qiong Wu, Yu Ye, Houjun He, Leyi Wang, Yuxin Tang
Porcine epidemic diarrhea virus (PEDV) is a devastating cause of diarrhea in pigs worldwide. Most of studies have focused on molecular and pathogenic characterization of PEDV, whereas there were limited studies in understanding the role of gut microbiota (GM) in viral-associated diarrhea. Here, using the Illumina MiSeq platform, we examined and compared the impact of PEDV infection on the GM of sows and their piglets less than 10 days old. Our results showed that PEDV caused alternations in the structure and abundance of GM from levels of phylum to genus, and even species...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29233857/timing-of-food-intake-impacts-daily-rhythms-of-human-salivary-microbiota-a-randomized-crossover-study
#13
María Carmen Collado, Phillip A Engen, Cristina Bandín, Raúl Cabrera-Rubio, Robin M Voigt, Stefan J Green, Ankur Naqib, Ali Keshavarzian, Frank A J L Scheer, Marta Garaulet
The composition of the diet (what we eat) has been widely related to the microbiota profile. However, whether the timing of food consumption (when we eat) influences microbiota in humans is unknown. A randomized, crossover study was performed in 10 healthy normal-weight young women to test the effect of the timing of food intake on the human microbiota in the saliva and fecal samples. More specifically, to determine whether eating late alters daily rhythms of human salivary microbiota, we interrogated salivary microbiota in samples obtained at 4 specific time points over 24 h, to achieve a better understanding of the relationship between food timing and metabolic alterations in humans...
December 12, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29228609/bacteriome-and-mycobiome-associations-in-oral-tongue-cancer
#14
Pranab K Mukherjee, Hannah Wang, Mauricio Retuerto, Huan Zhang, Brian Burkey, Mahmoud A Ghannoum, Charis Eng
Squamous cell carcinoma of the oral (mobile) tongue (OMTC), a non-human papilloma virus-associated oral cancer, is rapidly increasing without clear etiology. Poor oral hygiene has been associated with oral cancers, suggesting that oral bacteriome (bacterial community) and mycobiome (fungal community) could play a role. While the bacteriome is increasingly recognized as an active participant in health, the role of the mycobiome has not been studied in OMTC. Tissue DNA was extracted from 39 paired tumor and adjacent normal tissues from patients with OMTC...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29205917/targeted-in%C3%A2-situ-metatranscriptomics-for-selected-taxa-from-mesophilic-and-thermophilic-biogas-plants
#15
Yvonne Stolze, Andreas Bremges, Irena Maus, Alfred Pühler, Alexander Sczyrba, Andreas Schlüter
Biogas production is performed anaerobically by complex microbial communities with key species driving the process. Hence, analyses of their in situ activities are crucial to understand the process. In a previous study, metagenome sequencing and subsequent genome binning for different production-scale biogas plants (BGPs) resulted in four genome bins of special interest, assigned to the phyla Thermotogae, Fusobacteria, Spirochaetes and Cloacimonetes, respectively, that were genetically analysed. In this study, metatranscriptome sequencing of the same BGP samples was conducted, enabling in situ transcriptional activity determination of these genome bins...
December 4, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/29192628/a-comparative-study-of-the-oral-microbiome-compositions-of-healthy-postmenopausal-premenopausal-and-prepubertal-nigerian-females-using-16s-rrna-metagenomics-methods
#16
K C Anukam, N R Agbakoba
INTRODUCTION: There is a paucity of information on the oral microbiome compositions of Nigerians, mostly due to lack of appropriate molecular techniques. In this pilot study, we sought to determine and characterize the oral bacterial compositions of "healthy" females. MATERIALS AND METHODS: Oral samples were collected from three randomly selected females aged 56, 28, and 8 years. DNA was extracted and 16S rRNA V4 region was amplified using custom-barcoded primers before sequencing with Illumina MiSeq platform...
October 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29164415/decrease-of-insoluble-glucan-formation-in-streptococcus-mutans-by-co-cultivation-with-enterococcus-faecium-t7-and-glucanase-addition
#17
Shin-Hye Yu, So-Hyung Kwak, Thi Thanh Hanh Nguyen, Ye-Seul Seo, Chaeri Song, Il Kyoon Mok, Doman Kim
OBJECTIVES: To develop preventive canine oral health bio-materials consisting of probiotics and glucanase to reduce insoluble glucan and volatile sulfur compound formation. RESULTS: Co-cultivation of Enterococcus faecium T7 with Streptococcus mutans at inoculation ratio of 3:1 (v/v) resulted in 25% reduction in the growth of Streptococcus mutans. Amounts of soluble and insoluble glucans produced by S. mutans were decreased to 70 and 55%, respectively. Insoluble glucan was decreased from 0...
November 21, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29134144/composition-diversity-and-function-of-intestinal-microbiota-in-pacific-white-shrimp-litopenaeus-vannamei-at-different-culture-stages
#18
Shenzheng Zeng, Zhijian Huang, Dongwei Hou, Jian Liu, Shaoping Weng, Jianguo He
Intestinal microbiota is an integral component of the host and plays important roles in host health. The pacific white shrimp is one of the most profitable aquaculture species commercialized in the world market with the largest production in shrimp consumption. Many studies revealed that the intestinal microbiota shifted significantly during host development in other aquaculture animals. In the present study, 22 shrimp samples were collected every 15 days from larval stage (15 day post-hatching, dph) to adult stage (75 dph) to investigate the intestinal microbiota at different culture stages by targeting the V4 region of 16S rRNA gene, and the microbial function prediction was conducted by PICRUSt...
2017: PeerJ
https://www.readbyqxmd.com/read/29124770/characterization-of-the-gut-microbiota-in-early-life-stages-of-pikeperch-sander-lucioperca
#19
T Dulski, Z Zakęś, S Ciesielski
This study characterized the gastrointestinal microbiome of nine juvenile farmed pikeperch Sander lucioperca using a metagenomics approach based on bacterial 16S rRNA gene sequencing. Potential changes in the gut microbiota during 2 months of S. lucioperca juvenile life were investigated. Results revealed that gut microbiota was dominated by Proteobacteria (95-92%), while other phyla Firmicutes (1-1·5%) and Actinobacteria (0·9-1·5%) were less abundant. At the family level, fish-gut microbiota were dominated by Enterobacteriaceae, which constituted c...
November 9, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/29121118/diversity-and-characterization-of-bacteria-associated-with-the-deep-sea-hydrothermal-vent-crab-austinograea-sp-comparing-with-those-of-two-shallow-water-crabs-by-16s-ribosomal-dna-analysis
#20
COMPARATIVE STUDY
Na Zhang, Chengwen Song, Minxiao Wang, Yuan Liu, Min Hui, Zhaoxia Cui
For deep-sea hydrothermal vent crabs, recent investigations have revealed some epibiotic bacteria, but no study has described the bacterial community associated with the gill and intestine. In this study, the microbiota attached to the gill and intestine of the hydrothermal vent crab Austinograea sp. and two shallow-water crab species (Eriocheir sinensis and Portunus trituberculatus) were compared by high-throughput sequencing of 16S rDNA genes. The highest and lowest diversity in bacterial communities were observed in the gill and intestine of Austinograea sp...
2017: PloS One
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