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https://www.readbyqxmd.com/read/28220148/individual-signatures-define-canine-skin-microbiota-composition-and-variability
#1
Anna Cuscó, Armand Sánchez, Laura Altet, Lluís Ferrer, Olga Francino
Dogs present almost all their skin sites covered by hair, but canine skin disorders are more common in certain skin sites and breeds. The goal of our study is to characterize the composition and variability of the skin microbiota in healthy dogs and to evaluate the effect of the breed, the skin site, and the individual. We have analyzed eight skin sites of nine healthy dogs from three different breeds by massive sequencing of 16S rRNA gene V1-V2 hypervariable regions. The main phyla inhabiting the skin microbiota in healthy dogs are Proteobacteria, Firmicutes, Fusobacteria, Actinobacteria, and Bacteroidetes...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28183168/effects-of-alfalfa-flavonoids-extract-on-the-microbial-flora-of-dairy-cow-rumen
#2
Jinshun Zhan, Mingmei Liu, Caixia Wu, Xiaoshuang Su, Kang Zhan, Guo Qi Zhao
Objective: The effect of flavonoids from alfalfa on the microbial flora was determined using molecular techniques of 16S ribosome deoxyribonucleic acid (rDNA) analysis. Methods:Four primiparous Holstein heifers fitted with ruminal cannulas were used in a 4 × 4 Latin square design and fed a total mixed ration (TMR) to which alfalfa flavonoids extract (AFE) was added at the rates of 0 (A, control), 20 (B), 60 (C) or 100 (D) mg per kg of heifer body weight (BW) . Results: The number of OTUs in heifers given higher levels of flavonoid extract (C and D) was higher than for the two other treatments...
February 1, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28161185/the-bovine-colostrum-microbiome-and-its-association-with-clinical-mastitis
#3
Svetlana F Lima, Andre G V Teixeira, Fabio S Lima, Erika K Ganda, Catherine H Higgins, Georgios Oikonomou, Rodrigo C Bicalho
In an effort to characterize colostrum microbial diversity and its potential associations with early-lactation clinical mastitis, we used high-throughput sequencing of the 16S rRNA gene to investigate the bovine colostrum microbiome. A prospective observational study was conducted that included 70 Holstein cows; colostrum samples were collected from all 4 mammary gland quarters. Colostrum samples were categorized according to whether the quarter was diagnosed (CMC) or not diagnosed (NCMC) with clinical mastitis during the first 30 d postpartum...
February 1, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28124157/impact-of-the-microbiota-and-gastric-disease-development-by-helicobacter-pylori
#4
Teresa Alarcón, Laura Llorca, Guillermo Perez-Perez
Microorganisms in humans form complex communities with important functions and differences in each part of the body. The stomach was considered to be a sterile organ until the discovery of Helicobacter pylori, but nowadays, it is possible to demonstrate that other microorganisms beyond H. pylori can colonize the gastric mucosa and that the diverse microbiota ecosystem of the stomach is different from the mouth and the esophagus, and also from the small intestine and large intestine. H. pylori seems to be the most important member of the gastric microbiota with the highest relative abundance when present, but when it is absent, the stomach has a diverse microbiota...
2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28120199/a-comprehensive-profiling-of-supragingival-bacterial-composition-in-chinese-twin-children-and-their-mothers
#5
Qin Du, Mingyun Li, Xuedong Zhou, Kun Tian
To investigate the factors influencing the oral microbiome, 16 twin pairs and their mothers were enrolled in this study. There were 7 monozygotic (MZ) twins and 9 dizygotic (DZ) twins, with an average age of 6.18 ± 3.5. Supragingival plaques and caries tissues were collected and the 16S rDNA was analyzed by 454 pyrosequencing. A total of 18 phyla and 179 genuses were found. Caries children harbored a higher portion of Actinobacteria than caries-free ones, and one lineage was found to be less abundant in the caries tissues compared to the supragingival plaque at each level from the phylum down to the genus level, which was Fusobacteria at Phylum, Fusobacteriia at Class, Fusobacteriales at Order, Leptotrichiaceae at Family and Leptotrichia at genus level...
January 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28103814/the-respiratory-microbiome-in-bronchial-mucosa-and-secretions-from-severe-ige-mediated-asthma-patients
#6
Laura Millares, Guadalupe Bermudo, Vicente Pérez-Brocal, Christian Domingo, Marian Garcia-Nuñez, Xavier Pomares, Andrés Moya, Eduard Monsó
BACKGROUND: The bronchial microbiome in chronic lung diseases presents an abnormal pattern, but its microbial composition and regional differences in severe asthma have not been sufficiently addressed. The aim of the study was to describe the bacterial community in bronchial mucosa and secretions of patients with severe chronic asthma chronically treated with corticosteroids in addition to usual care according to Global Initiative for Asthma. Bacterial community composition was obtained by 16S rRNA gene amplification and sequencing, and functional capabilities through PICRUSt...
January 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28093882/the-gastrointestinal-tract-of-farmed-mink-neovison-vison-maintains-a-diverse-mucosa-associated-microbiota-following-a-3-day-fasting-period
#7
Martin I Bahl, Anne S Hammer, Tove Clausen, Anabelle Jakobsen, Søren Skov, Lars Andresen
Although it is well documented that the gut microbiota plays an important role in health and disease in mammalian species, this area has been poorly studied among carnivorous animals, especially within the mustelidae family. The gastrointestinal tract of carnivores is characterized by its short length and fast transit time, as compared to omnivores and herbivores, which is due to the low level of inherent fermentation. Mink represents an example of this, which have a GI tract only four times the length of the body and a transit time of approximately 4-5 hr...
January 16, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28058599/epiphytic-bacterial-communities-of-the-alga-fucus-vesiculosus-in-oil-contaminated-water-areas-of-the-barents-sea
#8
D V Pugovkin, A Liaimer, J B Jensen
Taxonomic compositions of epiphytic bacterial communities in water areas differing in levels of oil pollution were revealed. In total, 82 bacterial genera belonging to 16 classes and 11 phyla were detected. All detected representatives of epiphytic bacterial communities belonged to the phyla Actinobacteria, Bacteroidetes, Planctomycetes, Proteobacteria, Verrucomicrobia, Acidobacteria, Cyanobacteria, Firmicutes, and Fusobacteria and candidate division TM7. The ratio of the phyla in the communities varied depending on the levels of oil pollution...
November 2016: Doklady Biological Sciences: Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections
https://www.readbyqxmd.com/read/28024525/gut-content-microbiota-of-introduced-bigheaded-carps-hypophthalmichthys-spp-inhabiting-the-largest-shallow-lake-in-central-europe
#9
Andrea K Borsodi, Attila Szabó, Gergely Krett, Tamás Felföldi, András Specziár, Gergely Boros
Studying the microbiota in the alimentary tract of bigheaded carps (Hypophthalmichthys spp.) gained special interest recently, as these types of investigations on non-native fish species may lead to a better understanding of their ecological role and feeding habits in an invaded habitat. For microbiological examinations, bigheaded carp gut contents and water column samples from Lake Balaton (Hungary) were collected from spring to autumn in 2013. Denaturing Gradient Gel Electrophoresis (DGGE) and pyrosequencing of the 16S rRNA gene were performed to reveal the composition...
January 2017: Microbiological Research
https://www.readbyqxmd.com/read/27976528/streptococcus-mutans-spap-binds-to-radd-of-fusobacterium-nucleatum-ssp-polymorphum
#10
Lihong Guo, Bhumika Shokeen, Xuesong He, Wenyuan Shi, Renate Lux
Adhesin-mediated bacterial interspecies interactions are important elements in oral biofilm formation. They often occur on a species-specific level, which could determine health- or disease association of a biofilm community. Among the key players involved in these processes are the ubiquitous fusobacteria that have been recognized for their ability to interact with numerous different binding partners. Fusobacterial interactions with Streptococcus mutans, an important oral cariogenic pathogen, have previously been described but most studies focused on binding to non-mutans streptococci and specific cognate adhesin pairs remain to be identified...
December 15, 2016: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/27957630/characterization-of-gastric-microbiota-in-twins
#11
COMPARATIVE STUDY
Quanjiang Dong, Yongning Xin, Lili Wang, Xinying Meng, Xinjuan Yu, Linlin Lu, Shiying Xuan
Contribution of host genetic backgrounds in the development of gastric microbiota has not been clearly defined. This study was aimed to characterize the biodiversity, structure and composition of gastric microbiota among twins. A total of four pairs of twins and eight unrelated individuals were enrolled in the study. Antral biopsies were obtained during endoscopy. The bacterial 16S rRNA gene was amplified and pyrosequenced. Sequences were analyzed for the composition, structure, and α and β diversities of gastric microbiota...
February 2017: Current Microbiology
https://www.readbyqxmd.com/read/27894254/divergent-selection-induced-obesity-alters-the-composition-and-functional-pathways-of-chicken-gut-microbiota
#12
Jinmei Ding, Lele Zhao, Lifeng Wang, Wenjing Zhao, Zhengxiao Zhai, Li Leng, Yuxiang Wang, Chuan He, Yan Zhang, Heping Zhang, Hui Li, He Meng
BACKGROUND: The gastrointestinal tract is populated by a complex and vast microbial network, with a composition that reflects the relationships of the symbiosis, co-metabolism, and co-evolution of these microorganisms with their host. The mechanism that underlies such interactions between the genetics of the host and gut microbiota remains elusive. RESULTS: To understand how genetic variation of the host shapes the gut microbiota and interacts with it to affect the metabolic phenotype of the host, we compared the abundance of microbial taxa and their functional performance between two lines of chickens (fat and lean) that had undergone long-term divergent selection for abdominal fat pad weight, which resulted in a 4...
28, 2016: Genetics, Selection, Evolution: GSE
https://www.readbyqxmd.com/read/27846300/ontogenetic-characterization-of-the-intestinal-microbiota-of-channel-catfish-through-16s-rrna-gene-sequencing-reveals-insights-on-temporal-shifts-and-the-influence-of-environmental-microbes
#13
Jacob W Bledsoe, Brian C Peterson, Kelly S Swanson, Brian C Small
Aquaculture recently overtook capture fisheries as the largest producer of food fish, but to continue increasing fish production the industry is in search of better methods of improving fish health and growth. Pre- and probiotic supplementation has gained attention as a means of solving these issues, however, for such approaches to be successful, we must first gain a more holistic understanding of the factors influencing the microbial communities present in the intestines of fish. In this study, we characterize the bacterial communities associated with the digestive tract of a highly valuable U...
2016: PloS One
https://www.readbyqxmd.com/read/27799062/middle-ear-microbiome-differences-in-indigenous-filipinos-with-chronic-otitis-media-due-to-a-duplication-in-the-a2ml1-gene
#14
Regie Lyn P Santos-Cortez, Diane S Hutchinson, Nadim J Ajami, Ma Rina T Reyes-Quintos, Ma Leah C Tantoco, Patrick John Labra, Sheryl Mae Lagrana, Melquiadesa Pedro, Erasmo Gonzalo D V Llanes, Teresa Luisa Gloria-Cruz, Abner L Chan, Eva Maria Cutiongco-de la Paz, John W Belmont, Tasnee Chonmaitree, Generoso T Abes, Joseph F Petrosino, Suzanne M Leal, Charlotte M Chiong
BACKGROUND: Previously rare A2ML1 variants were identified to confer otitis media susceptibility in an indigenous Filipino community and in otitis-prone US children. The goal of this study is to describe differences in the middle ear microbiome between carriers and non-carriers of an A2ML1 duplication variant that increases risk for chronic otitis media among indigenous Filipinos with poor health care access. METHODS: Ear swabs were obtained from 16 indigenous Filipino individuals with chronic otitis media, of whom 11 carry the A2ML1 duplication variant...
November 1, 2016: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/27797671/nutritional-correlates-of-human-oral-microbiome
#15
Ikuko Kato, Adrian Vasquez, Gregory Moyerbrailean, Susan Land, Zora Djuric, Jun Sun, Ho-Sheng Lin, Jeffrey L Ram
BACKGROUND: Despite many potential effects of the oral microbiome on oral and systemic health, scant information is available regarding the associations between diet and the oral microbiome. METHODS: Oral rinse DNA samples from 182 participants in a population-based case-control study for colorectal cancer were used to amplify a V3-V4 region of bacterial 16S rRNA gene. The amplicons were sequenced using Illumina MiSeq paired end chemistry on 2 runs, yielding approximately 33 million filtered reads that were assigned to bacterial classes...
February 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/27742762/human-oral-microbiome-and-prospective-risk-for-pancreatic-cancer-a-population-based-nested-case-control-study
#16
Xiaozhou Fan, Alexander V Alekseyenko, Jing Wu, Brandilyn A Peters, Eric J Jacobs, Susan M Gapstur, Mark P Purdue, Christian C Abnet, Rachael Stolzenberg-Solomon, George Miller, Jacques Ravel, Richard B Hayes, Jiyoung Ahn
OBJECTIVE: A history of periodontal disease and the presence of circulating antibodies to selected oral pathogens have been associated with increased risk of pancreatic cancer; however, direct relationships of oral microbes with pancreatic cancer have not been evaluated in prospective studies. We examine the relationship of oral microbiota with subsequent risk of pancreatic cancer in a large nested case-control study. DESIGN: We selected 361 incident adenocarcinoma of pancreas and 371 matched controls from two prospective cohort studies, the American Cancer Society Cancer Prevention Study II and the National Cancer Institute Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial...
October 14, 2016: Gut
https://www.readbyqxmd.com/read/27734626/comparative-analysis-of-the-fecal-bacterial-community-of%C3%A2-five-harbor-seals-phoca-vitulina
#17
Daniela Numberger, Daniel P R Herlemann, Klaus Jürgens, Guido Dehnhardt, Heide Schulz-Vogt
The gut microbiota has many beneficial effects on host metabolism and health, and its composition is determined by numerous factors. It is also assumed that there was a co-evolution of mammals and the bacteria inhabiting their gut. Current knowledge of the mammalian gut microbiota mainly derives from studies on humans and terrestrial animals, whereas those on marine mammals are sparse. However, they could provide additional information on influencing factors, such as the role of diet and co-evolution with the host...
October 2016: MicrobiologyOpen
https://www.readbyqxmd.com/read/27732961/the-microbial-community-shifts-of-subgingival-plaque-in-patients-with-generalized-aggressive-periodontitis-following-non-surgical-periodontal-therapy-a-pilot-study
#18
Jing Han, Peng Wang, Shaohua Ge
The object of this study is to characterize the bacterial community of subgingival plaque of two subjects with generalized aggressive periodontitis (GAgP) pre- and post-treatment. We picked two patients with GAgP and used high-throughput 16S rDNA sequencing. V4 hypervariable region was picked for PCR amplification of subgingival samples. Then, the PCR products were sequenced through Illumina MiSeq platform. One month after therapy, both the clinical features and periodontal parameters improved obviously. Moreover, the composition and structure of subgingival bacterial community changed after initial periodontal therapy...
February 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/27701432/influence-of-endogenous-and-exogenous-estrogenic-endocrine-on-intestinal-microbiota-in-zebrafish
#19
Yukun Liu, Yayun Yao, Huan Li, Fang Qiao, Junlin Wu, Zhen-Yu Du, Meiling Zhang
Gender is one of the factors influencing the intestinal microbial composition in mammals, but whether fish also have gender-specific intestinal microbial patterns remains unknown. In this decade, endocrine disrupting chemicals in surface and ground water of many areas and increasing observation of freshwater male fish displaying female sexual characteristics have been reported. Here we identified the difference in intestinal microbiota between male and female zebrafish, and revealed the influence of endocrine disrupting chemicals on zebrafish intestinal microbiota by using high-throughput sequencing...
2016: PloS One
https://www.readbyqxmd.com/read/27695351/lemierre-s-syndrome-current-perspectives-on-diagnosis-and-management
#20
REVIEW
Katrine M Johannesen, Uffe Bodtger
This is a systematic review of cases with Lemierre's syndrome (LS) in the past 5 years. LS is characterized by sepsis often evolving after a sore throat or tonsillitis and then complicated by various septic emboli and thrombosis of the internal jugular vein. Symptoms include sepsis, pain, and/or swelling in the throat or neck, as well as respiratory symptoms. Laboratory findings show elevated infectious parameters and radiological findings show thrombosis of the internal jugular vein and emboli in the lungs or other organs...
2016: Infection and Drug Resistance
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