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https://www.readbyqxmd.com/read/28823044/diagnostic-implications-of-tert-promoter-mutation-status-in-diffuse-gliomas-in-a-routine-clinical-setting
#1
Ekkehard Hewer, Nadine Prebil, Sabina Berezowska, Marielena Gutt-Will, Philippe Schucht, Matthias S Dettmer, Erik Vassella
IDH (isocitrate dehydrogenase) gene mutations are present in most diffuse low-grade gliomas and define the clinico-pathological core of the respective morphologically defined entities. Conversely, according to the 2016 WHO classification, the majority of glioblastomas belong to the IDH-wildtype category, which is defined by exclusion. TERT (telomerase reverse transcriptase gene) promoter mutations have been suggested as a molecular marker for primary glioblastomas. We analyzed molecular, histopathological, and clinical profiles of a series of 110 consecutive diffuse gliomas (WHO grades II-IV) diagnosed at our institution, in which TERT promoter mutation analysis had been performed as part of diagnostic work-up...
August 19, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28822947/cell-metabolomics-reveals-the-neurotoxicity-mechanism-of-cadmium-in-pc12-cells
#2
Li Zong, Junpeng Xing, Shu Liu, Zhiqiang Liu, Fengrui Song
The heavy metals such as cadmium (Cd) can induce neurotoxicity. Extensive studies about the effects of Cd on human health have been reported, however, a systematic investigation on the molecular mechanisms of the effects of Cd on central nervous system is still needed. In this paper, the neuronal PC-12 cells were treated with a series of concentrations of CdCl2 for 48h. Then the cytotoxicity was evaluated by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. The IC15 value (15% inhibiting concentration) was selected for further mechanism studies...
August 17, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28822354/3d-brain-organoids-derived-from-pluripotent-stem-cells-promising-experimental-models-for-brain-development-and-neurodegenerative-disorders
#3
REVIEW
Chun-Ting Lee, Raphael M Bendriem, Wells W Wu, Rong-Fong Shen
Three-dimensional (3D) brain organoids derived from human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), appear to recapitulate the brain's 3D cytoarchitectural arrangement and provide new opportunities to explore disease pathogenesis in the human brain. Human iPSC (hiPSC) reprogramming methods, combined with 3D brain organoid tools, may allow patient-derived organoids to serve as a preclinical platform to bridge the translational gap between animal models and human clinical trials...
August 20, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28822308/the-acute-effects-of-ultraviolet-radiation-on-the-blood-transcriptome-are-independent-of-plasma-25ohd3
#4
Mariona Bustamante, Carles Hernandez-Ferrer, Yaris Sarria, Graham I Harrison, Lara Nonell, Wenjing Kang, Marc R Friedländer, Xavier Estivill, Juan R González, Mark Nieuwenhuijsen, Antony R Young
The molecular basis of many health outcomes attributed to solar ultraviolet radiation (UVR) is unknown. We tested the hypothesis that they may originate from transcriptional changes in blood cells. This was determined by assessing the effect of fluorescent solar simulated radiation (FSSR) on the transcriptional profile of peripheral blood pre- and 6h, 24h and 48h post-exposure in nine healthy volunteers. Expression of 20 genes was down-regulated and one was up-regulated at 6h after FSSR. All recovered to baseline expression at 24h or 48h...
August 16, 2017: Environmental Research
https://www.readbyqxmd.com/read/28822241/transcript-analysis-identifies-differential-uterine-gene-expression-profile-beyond-the-normal-implantation-window-in-mice
#5
Qiliang Xin, Meiling Li, Haibin Wang, Sheng Cui, Guoliang Xia, Shuangbo Kong
Uterine receptivity is defined as a state when the uterine milieu is favorable for blastocyst implantation and it can only last for a limited time period. In this study, by utilizing the embryo transfer model, it was observed that a portion of blastocysts could initiate implantation even when transferred beyond the timing of normal uterine receptivity, while their mid-gestational embryo development exhibited severe retardation, suggesting that the uterine status beyond the normal implantation window is unconducive for normal implantation...
August 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822204/comparative-2-d-electrophoresis-of-salivary-proteins-in-triatoma-dimidiata-and-rhodnius-prolixus-hemiptera-reduviidae-and-major-cross-reactive-antigens
#6
Victor Monteon, Ivan May-Gil, Karla Acosta-Viana, Angel Ramos-Logonio, Oscar Hernandez, Ruth Lopez
An immune response to triatomine’s saliva is an immunological marker of exposure to triatomine bites. However, considerable variability in salivary protein profiles did exist among species. In the present work, we compare salivary proteins from Mexican Triatoma dimidiata and Rhodnius prolixus using 2-D electrophoresis. A clear differential saliva profile was found to exist between these two triatomine species. Fewer protein spots were detected in R. prolixus than in T. dimidiata. More than half of the proteins had an isoelectric point between 5 and 7 and a molecular weight between 10 and 30 kDa in T...
2017: Annals of Parasitology
https://www.readbyqxmd.com/read/28822081/expression-profiling-of-the-map-kinase-phosphatase-family-reveals-a-role-for-dusp1-in-the-glioblastoma-stem-cell-niche
#7
Bradley N Mills, George P Albert, Marc W Halterman
The dual specificity phosphatases (DUSPs) constitute a family of stress-induced enzymes that provide feedback inhibition on mitogen-activated protein kinases (MAPKs) critical in key aspects of oncogenic signaling. While described in other tumor types, the landscape of DUSP mRNA expression in glioblastoma (GB) remains largely unexplored. Interrogation of the REpository for Molecular BRAin Neoplasia DaTa (REMBRANDT) revealed induction (DUSP4, DUSP6), repression (DUSP2, DUSP7-9), or mixed (DUSP1, DUSP5, DUSP10, DUSP15) DUSP transcription of select DUSPs in bulk tumor specimens...
August 18, 2017: Cancer Microenvironment: Official Journal of the International Cancer Microenvironment Society
https://www.readbyqxmd.com/read/28821798/unconventional-mapk-gsk-3%C3%AE-pathway-behind-atypical-epithelial-mesenchymal-transition-in-hepatocellular-carcinoma
#8
Sana Parveen, Dhiviya Vedagiri, Hitha Gopalan Nair, Haripriya Parthasarathy, Krishnan Harinivas Harshan
We recently reported an atypical epithelial mesenchymal transition (EMT) in human hepatoma cell culture Huh7.5, which was non-responsive to the canonical EMT-transcription factors. Here we characterize major pathways regulating this atypical EMT through whole genome transcriptome profiling and molecular analysis, and identify a unique regulation of EMT by GSK-3β. Our analysis reveals remarkable suppression of several key liver-specific markers in Huh7.5M cells indicating that EMT not only changes the epithelial properties, but alters the characteristics associated with hepatocytes as well...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821665/molecular-and-functional-properties-of-regional-astrocytes-in-the-adult-brain
#9
Lydie Morel, Ming Sum R Chiang, Haruki Higashimori, Temitope Shoneye, Lakshmanan K Iyer, Julia Yelick, Albert Tai, Yongjie Yang
The molecular signature and functional properties of astroglial subtypes in the adult central nervous system (CNS) remain largely undefined. By employing translational ribosome affinity purification (TRAP) followed by RNA-seq, we profiled astroglial ribosome-associated (presumably translating) mRNAs in major cortical and subcortical brain regions (cortex, hippocampus, caudate putamen, nucleus accumbens, thalamus, and hypothalamus) of BAC aldh1l1-TRAP mice (both sexes). We found that the expression of astroglial translating mRNAs closely follows the dorsoventral axis especially from cortex/hippocampus to thalamus/hypothalamus posteriorly...
August 7, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28821643/changes-in-the-excitability-of-neocortical-neurons-in-a-mouse-model-of-amyotrophic-lateral-sclerosis-are-not-specific-to-corticospinal-neurons-and-are-modulated-by-advancing-disease
#10
Juhyun Kim, Ethan G Hughes, Ashwin S Shetty, Paola Arlotta, Loyal A Goff, Dwight E Bergles, Solange P Brown
Cell-type specific changes in neuronal excitability have been proposed to contribute to the selective degeneration of corticospinal neurons in amyotrophic lateral sclerosis (ALS) and to neocortical hyperexcitability, a prominent feature of both inherited and sporadic variants of the disease, but the mechanisms underlying selective loss of specific cell types in ALS are not known. We analyzed the physiological properties of distinct classes of cortical neurons in the motor cortex of hSOD1(G93A) mice of both sexes and found that they all exhibit increases in intrinsic excitability that depend on disease stage...
August 17, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28821607/two-plant-derived-aporphinoid-alkaloids-exert-their-antifungal-activity-by-disrupting-mitochondrial-iron-sulfur-cluster-biosynthesis
#11
Siddharth K Tripathi, Tao Xu, Qin Feng, Bharathi Avula, Xiaomin Shi, Xuewen Pan, Melanie M Mask, Scott R Baerson, Melissa R Jacob, Ranga Rao Ravu, Shabana I Khan, Xing-Cong Li, Ikhlas A Khan, Alice M Clark, Ameeta K Agarwal
Eupolauridine and liriodenine are plant-derived aporphinoid alkaloids that exhibit potent inhibitory activity against the opportunistic fungal pathogens Candida albicans and Cryptococcus neoformans However, the molecular mechanism of this antifungal activity is unknown. In this study, we show that eupolauridine 9591 (E9591), a synthetic analog of eupolauridine, and liriodenine methiodide (LMT), a methiodide salt of liriodenine, mediate their antifungal activities by disrupting mitochondrial iron-sulfur (Fe-S) cluster synthesis...
August 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28821602/large-scale-proteomics-combined-with-transgenic-experiments-demonstrates-an-important-role-of-jasmonic-acid-nbsp-in-potassium-nbsp-deficiency-response-in-wheat-and-rice
#12
Gezi Li, Yufang Wu, Guoyu Liu, Xianghong Xiao, Pengfei Wang, Tian Gao, Mengjun Xu, Qiaoxia Han, Yonghua Wang, Tiancai Guo, Guozhang Kang
Potassium (K+) is the most abundant inorganic cation in plants, and molecular dissection of K+ deficiency has received considerable interest in order to minimize K+ fertilizer input and develop high quality K+-efficient crops. However, the molecular mechanism of plant responses to K+ deficiency is still poorly understood. In this study, two-week-old bread wheat seedlings grown hydroponically in Hoagland solution were transferred to K+-free conditions for 8 d, and their root and leaf proteome profiles were assessed using the iTRAQ proteome method...
August 18, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28821299/pknb-remains-an-essential-and-a-conserved-target-for-drug-development-in-susceptible-and-mdr-strains-of-m-tuberculosis
#13
Anamika Gupta, Sudhir K Pal, Divya Pandey, Najneen A Fakir, Sunita Rathod, Dhiraj Sinha, S SivaKumar, Pallavi Sinha, Mycal Periera, Shilpa Balgam, Gomathi Sekar, K R UmaDevi, Shampa Anupurba, Vijay Nema
BACKGROUND: The Mycobacterium tuberculosis (M.tb) protein kinase B (PknB) which is now proved to be essential for the growth and survival of M.tb, is a transmembrane protein with a potential to be a good drug target. However it is not known if this target remains conserved in otherwise resistant isolates from clinical origin. The present study describes the conservation analysis of sequences covering the inhibitor binding domain of PknB to assess if it remains conserved in susceptible and resistant clinical strains of mycobacteria picked from three different geographical areas of India...
August 18, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28821232/genomic-transcriptomic-and-proteomic-approaches-towards-understanding-the-molecular-mechanisms-of-salt-tolerance-in-frankia-strains-isolated-from-casuarina-trees
#14
Rediet Oshone, Mariama Ngom, Feixia Chu, Samira Mansour, Mame Ourèye Sy, Antony Champion, Louis S Tisa
BACKGROUND: Soil salinization is a worldwide problem that is intensifying because of the effects of climate change. An effective method for the reclamation of salt-affected soils involves initiating plant succession using fast growing, nitrogen fixing actinorhizal trees such as the Casuarina. The salt tolerance of Casuarina is enhanced by the nitrogen-fixing symbiosis that they form with the actinobacterium Frankia. Identification and molecular characterization of salt-tolerant Casuarina species and associated Frankia is imperative for the successful utilization of Casuarina trees in saline soil reclamation efforts...
August 18, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28821226/comprehensive-proteome-profiling-in-aedes-albopictus-to-decipher-wolbachia-arbovirus-interference-phenomenon
#15
Yoann Saucereau, Claire Valiente Moro, Cindy Dieryckx, Jean-William Dupuy, Florence-Hélène Tran, Vincent Girard, Patrick Potier, Patrick Mavingui
BACKGROUND: Aedes albopictus is a vector of arboviruses that cause severe diseases in humans such as Chikungunya, Dengue and Zika fevers. The vector competence of Ae. albopictus varies depending on the mosquito population involved and the virus transmitted. Wolbachia infection status in believed to be among key elements that determine viral transmission efficiency. Little is known about the cellular functions mobilized in Ae. albopictus during co-infection by Wolbachia and a given arbovirus...
August 18, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28820711/molecular-epidemiology-of-cholera-outbreaks-during-the-rainy-season-in-mandalay-myanmar
#16
Amonrattana Roobthaisong, Kazuhisa Okada, Nilar Htun, Wah Wah Aung, Warawan Wongboot, Watcharaporn Kamjumphol, Aye Aye Han, Yi Yi, Shigeyuki Hamada
Cholera, caused by Vibrio cholerae, remains a global threat to public health. In Myanmar, the availability of published information on the occurrence of the disease is scarce. We report here that cholera incidence in Mandalay generally exhibited a single annual peak, with an annual average of 312 patients with severe dehydration over the past 5 years (since 2011) and was closely associated with the rainy season. We analyzed cholera outbreaks, characterized 67 isolates of V. cholerae serogroup O1 in 2015 from patients from Mandalay, and compared them with 22 V...
August 14, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820406/-results-of-preparation-and-implementation-of-ivf-program-in-patients-with-thrombophilia-and-history-of-failed-ivf
#17
G Abrahamyan
The problem of infertility and reproductive losses maintains its urgency, as well as medical and social significance. Frequency of infertility in overall population, according to the data from different authors, varies from 9 to 18 per cent. Methods of aided reproductive technologies (ART) opened a new era in the field of correction of infertile marriage. As a result, more and more couples choose to solve this problem by means of aided reproductive technologies (ART): in-vitro fertilization (IVF) and embryo transfer (ET)...
July 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28820289/recent-advances-in-quantitative-and-chemical-proteomics-for-autophagy-studies
#18
Yin-Kwan Wong, Jianbin Zhang, Zi-Chun Hua, Qingsong Lin, Han-Ming Shen, Jigang Wang
Macroautophagy/autophagy is an evolutionarily well-conserved cellular degradative process with important biological functions that is closely implicated in health and disease. In recent years, quantitative mass spectrometry-based proteomics and chemical proteomics have emerged as important tools for the study of autophagy, through large-scale unbiased analysis of the proteome or through highly specific and accurate analysis of individual proteins of interest. At present, a variety of approaches have been successfully applied, including (i) expression and interaction proteomics for the study of protein post-translational modifications, (ii) investigating spatio-temporal dynamics of protein synthesis and degradation, and (iii) direct determination of protein activity and profiling molecular targets in the autophagic process...
August 18, 2017: Autophagy
https://www.readbyqxmd.com/read/28820086/stenotrophomonas-bentonitica-sp-nov-isolated-from-bentonite-formations
#19
Iván Sánchez-Castro, Miguel Angel Ruiz-Fresneda, Mohammed Bakkali, Peter Kämpfer, Stefanie P Glaeser, Hans Jürgen Busse, Margarita López-Fernández, Pablo Martínez-Rodríguez, Mohamed Larbi Merroun
A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7T, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence analysis showed that BII-R7T represented a member of the genus Stenotrophomonas (class Gammaproteobacteria), and was related most closely to Stenotrophomonas rhizophila e-p10T (99.2 % sequence similarity), followed by Stenotrophomonas pavanii ICB 89T (98.5 %), Stenotrophomonas maltophilia IAM 12423T, Stenotrophomonas chelatiphaga LPM-5T and Stenotrophomonas tumulicola T5916-2-1bT (all 98...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820051/bisthiourea-derivatives-of-dipeptide-conjugated-benzo-d-isoxazole-as-a-new-class-of-therapeutics-synthesis-and-molecular-docking-studies
#20
H K Kumara, D M Suyoga Vardhan, J Shiva Kumar, D Channe Gowda
We have synthesized a series of bisthiourea derivatives of dipeptides (Lys/lys-Asp and Lys/lys-Trp) conjugated benzo[d]isoxazole and the structures of synthesized compounds were confirmed by correlating their physical and spectroscopic data with earlier data. The molecules 1-24 were examined for their in vitro anti-inflammatory activity by employing human erythrocyte suspension test and it was found that the IC50 values of 9, 12, 21 and 24 were lower than the IC50 of standard references indomethacin and ibuprofen...
August 16, 2017: Anti-inflammatory & Anti-allergy Agents in Medicinal Chemistry
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