keyword
MENU ▼
Read by QxMD icon Read
search

Molecular profiling

keyword
https://www.readbyqxmd.com/read/29241027/when-forensic-odontology-met-biochemistry-multidisciplinary-approach-in-forensic-human-identification
#1
REVIEW
Joe Adserias-Garriga, Christian Thomas, Douglas H Ubelaker, Sara C Zapico
When human remains are found, the priority of the investigation is to ascertain the identity of the deceased. A positive identification is a key factor in providing closure for the family of the deceased; it is also required to issue the death certificate and therefore, to settle legal affairs. Moreover, it is difficult for any forensic investigation involving human remains to be solved without the determination of an identity. Therefore, personal identification is necessary for social, legal and forensic reasons...
December 9, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29240458/genome-wide-analysis-of-uveal-melanoma-metastasis-associated-lncrnas-and-their-functional-network
#2
Binbin Xu, Ruiqi Ma, Hui Ren, Jiang Qian
Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. Up to 50% of primary UM cases will develop distant metastasis, but no effective therapies are currently available. The present study aimed to characterize the expression profile of the long noncoding RNAs (lncRNAs) and screen the potential metastasis-associated lncRNAs in UM. A genome-wide analysis of the transcriptome was performed on 11 primary UM tissues (6 metastasized and 5 nonmetastasized) through RNA sequencing. A total of 40,878 lncRNAs were detected in UM, 4,983 of which were novel candidates...
December 14, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/29239603/thermodynamics-of-amyloid-%C3%AE-fibril-elongation-atomistic-details-of-the-transition-state
#3
Roberto A Rodriguez, Liao Y Chen, Germán Plascencia-Villa, George Perry
Amyloid-β (Aβ) fibrils and plaques are one of the hallmarks of Alzheimer's disease. While the kinetics of fibrillar growth of Aβ have been extensively studied, several vital questions remain. In particular, the atomistic origins of the Arrhenius barrier observed in experiments have not been elucidated. Employing the familiar thermodynamic integration method, we have directly simulated the dissociation of an Aβ(15-40) (D23N mutant) peptide from the surface of a filament along its most probable path (MPP) using all-atom molecular dynamics...
December 14, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29239310/micrornas-as-diagnostic-markers-and-therapeutic-targets-for-traumatic-brain-injury
#4
REVIEW
Bridget Martinez, Philip V Peplow
Traumatic brain injury (TBI) is characterized by primary damage to the brain from the external mechanical force and by subsequent secondary injury due to various molecular and pathophysiological responses that eventually lead to neuronal cell death. Secondary brain injury events may occur minutes, hours, or even days after the trauma, and provide valuable therapeutic targets to prevent further neuronal degeneration. At the present time, there is no effective treatment for TBI due, in part, to the widespread impact of numerous complex secondary biochemical and pathophysiological events occurring at different time points following the initial injury...
November 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/29238707/drug-repurposing-for-the-treatment-of-acute-myeloid-leukemia
#5
REVIEW
Vibeke Andresen, Bjørn T Gjertsen
Acute myeloid leukemia (AML) is a heterogeneous disease characterized by the accumulation of immature myeloid progenitor cells in the bone marrow, compromising of normal blood cell production and ultimately resulting in bone marrow failure. With a 20% overall survival rate at 5 years and 50% in the 18- to 65-year-old age group, new medicines are needed. It is proposed that development of repurposed drugs may be a part of the new therapy needed. AML is subdivided into recurrent molecular entities based on molecular genetics increasingly accessible for precision medicine...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/29238371/application-of-the-amlprofiler-diagnostic-microarray-in-the-south-african-setting
#6
S S Kappala, M Alessandrini, T Matlhako, E Beltchev, R Pool, M S Pepper
Acute myeloid leukemia (AML) is characterized by proliferation of the myeloid lineage and accumulation of immature hematopoietic cells in the bone marrow and is typified by marked heterogeneity both in response to treatment and survival. AMLprofiler is a qualitative in vitro diagnostic microarray incorporating seven molecular biomarkers used to diagnose and predict posttherapy survival rates. In this study, we compared AMLprofiler to routine AML diagnostic methodologies employed in South Africa, focusing on consistency of the results, cost, and time to result...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29238047/negative-control-of-the-hgf-c-met-pathway-by-tgf-%C3%AE-a-new-look-at-the-regulation-of-stemness-in-glioblastoma
#7
Eleanna Papa, Michael Weller, Tobias Weiss, Elisa Ventura, Isabel Burghardt, Emese Szabó
Multiple target inhibition has gained considerable interest in combating drug resistance in glioblastoma, however, understanding the molecular mechanisms of crosstalk between signaling pathways and predicting responses of cancer cells to targeted interventions has remained challenging. Despite the significant role attributed to transforming growth factor (TGF)-β family and hepatocyte growth factor (HGF)/c-MET signaling in glioblastoma pathogenesis, their functional interactions have not been well characterized...
December 13, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29237833/neutralizing-activity-of-broadly-neutralizing-anti-hiv-1-antibodies-against-clade-b-clinical-isolates-produced-in-peripheral-blood-mononuclear-cells
#8
Yehuda Z Cohen, Julio C C Lorenzi, Michael S Seaman, Lilian Nogueira, Till Schoofs, Lisa Krassnig, Allison Butler, Katrina Millard, Tomas Fitzsimons, Xiaoju Daniell, Juan P Dizon, Irina Shimeliovich, David C Montefiori, Marina Caskey, Michel C Nussenzweig
Recently discovered broadly neutralizing anti-HIV-1 antibodies (bNAbs) demonstrate extensive breadth and potency against diverse HIV-1 strains, and represent a promising approach for the treatment and prevention of HIV-1 infection. The breadth and potency of these antibodies have primarily been evaluated using panels of HIV-1 Env-pseudotyped viruses produced in 293T cells expressing molecularly cloned Envs. Here we report on the ability of 5 bNAbs currently in clinical development to neutralize circulating primary HIV-1 isolates derived from peripheral blood mononuclear cells (PBMC), and compare the results to the pseudovirus panels used to characterize the bNAbs...
December 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29237735/the-expanding-toolkit-of-translating-ribosome-affinity-purification
#9
Joseph D Dougherty
Translating ribosome affinity purification is a method initially developed for profiling mRNA from genetically defined cell types in complex tissues. It has been applied both to identify target molecules in cell types that are important for controlling a variety of behaviors in the brain, and to understand the molecular consequences on those cells due to experimental manipulations, ranging from drugs of abuse to disease-causing mutations. Since its inception, a variety of methodological advances are opening new avenues of investigation...
December 13, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29237679/statin-effects-on-metabolic-profiles-data-from-the-prevend-it-prevention-of-renal-and-vascular-end-stage-disease-intervention-trial
#10
Daniel Kofink, Ruben N Eppinga, Wiek H van Gilst, Stephan J L Bakker, Robin P F Dullaart, Pim van der Harst, Folkert W Asselbergs
BACKGROUND: Statins lower cholesterol by inhibiting HMG-CoA reductase, the rate-limiting enzyme of the metabolic pathway that produces cholesterol and other isoprenoids. Little is known about their effects on metabolite and lipoprotein subclass profiles. We, therefore, investigated the molecular changes associated with pravastatin treatment compared with placebo administration using a nuclear magnetic resonance-based metabolomics platform. METHODS AND RESULTS: We performed metabolic profiling of 231 lipoprotein and metabolite measures in the PREVEND IT (Prevention of Renal and Vascular End-stage Disease Intervention Trial) study, a placebo-controlled randomized clinical trial designed to test the effects of pravastatin (40 mg once daily) on cardiovascular risk...
December 2017: Circulation. Cardiovascular Genetics
https://www.readbyqxmd.com/read/29237567/integrated-metabolomics-and-proteomics-analysis-of-hippocampus-in-a-rat-model-of-depression
#11
Yuqing Zhang, Shuai Yuan, Juncai Pu, Lining Yang, Xinyu Zhou, Lanxiang Liu, Xiaofeng Jiang, Hanping Zhang, Teng Teng, Lu Tian, Peng Xie
Major depressive disorder (MDD) is a prevalent and serious mental disorder with high rates of suicide and disability. However, the underlying pathogenesis of MDD is complicated and remains largely unclear. An integrated analysis of multiple types of omics data may improve comprehensive understanding of the entire molecular mechanism of MDD. In this study, we applied an integrated analysis of gas chromatography/mass spectrometry (GC-MS)-based metabolomics and isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics to investigate changes in the hippocampus in the chronic unpredictable mild stress (CUMS) rat model of depression...
December 10, 2017: Neuroscience
https://www.readbyqxmd.com/read/29237426/long-non-coding-rna-malat1-protects-preterm-infants-with-bronchopulmonary-dysplasia-by-inhibiting-cell-apoptosis
#12
Cheng Cai, Jiajun Qiu, Gang Qiu, Yihuan Chen, Zhijun Song, Juan Li, Xiaohui Gong
BACKGROUND: Bronchopulmonary dysplasia (BPD) is a neonatal chronic lung disease characterized by impaired pulmonary alveolar development in preterm infants. Until now, little is known about the molecular and cellular basis of BPD. There is increasing evidence that lncRNAs regulate cell proliferation and apoptosis during lung organogenesis. The potential role of lncRNAs in the pathogenesis of BPD is unclear. This study aims to clarify the role of MALAT1 during the process of BPD in preterm infants and illustrate the protective effect of MALAT1 involved in preterm infants...
December 13, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/29237412/evaluation-of-epidermal-growth-factor-receptor-signaling-effects-in-gastric-cancer-cell-lines-by-detailed-motility-focused-phenotypic-characterization-linked-with-molecular-analysis
#13
Simone Keller, Julia Kneissl, Verena Grabher-Meier, Stefan Heindl, Jan Hasenauer, Dieter Maier, Julian Mattes, Peter Winter, Birgit Luber
BACKGROUND: Gastric cancers frequently overexpress the epidermal growth factor receptor (EGFR), which has been implicated in pathological processes including tumor cell motility, invasion and metastasis. Targeting EGFR with the inhibitory antibody cetuximab may affect the motile and invasive behavior of tumor cells. Here, we evaluated the effects of EGFR signaling in gastric cancer cell lines to link the phenotypic behavior of the cells with their molecular characteristics. METHODS: Phenotypic effects were analyzed in four gastric cancer cell lines (AGS, Hs746T, LMSU and MKN1) by time-lapse microscopy and transwell invasion assay...
December 13, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29237260/explaining-the-transition-from-diffusion-limited-to-reaction-limited-surface-assembly-of-molecular-species-through-spatial-variation
#14
Keith M Carroll, Armin W Knoll, Heiko Wolf, Urs Duerig
Surface assembly is often decomposed into two classes: reaction and diffusion limited processes. The transition between the two cases is complex because the dynamics for the two cases are so different. In this letter, we simulate, explain, and experimentally discuss the evolution of the spatial distribution for surface assemblies with diffusion limited and reaction limited processes. Explicitly, we demonstrate that diffusion limited processes and reaction limited process show some temporal differences, but more importantly, we show the spatial arrangement between the two scenarios are different enough to discriminate between the two cases...
December 14, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29237170/functional-impact-of-novel-androgen-receptor-mutations-on-the-clinical-manifestation-of-androgen-insensitivity-syndrome
#15
Reginaldo J Petroli, Olaf Hiort, Dagmar Struve, Julia K Gesing, Fernanda C Soardi, Angela M Spínola-Castro, Karla Melo, Ivo J Prado Arnhold, Andréa T Maciel-Guerra, Gil Guerra-Junior, Ralf Werner, Maricilda P de Mello
Androgens are responsible for the development and maintenance of male sex characteristics. Dysfunctions in androgen action due to mutations in the androgen receptor gene (AR) can lead to androgen insensitivity syndrome (AIS) that can be classified as mild (MAIS), partial (PAIS), or complete (CAIS). We have analyzed functional effects of p.Ser760Thr, p.Leu831Phe, p.Ile899Phe, p.Leu769Val, and p.Pro905Arg mutations and the combination p.Gln799Glu + p.Cys807Phe that were identified in patients with PAIS or CAIS...
December 14, 2017: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
https://www.readbyqxmd.com/read/29236939/a-proposal-to-explain-how-the-circatidal-rhythm-of-the-arabidopsis-thaliana-root-elongation-rate-could-be-mediated-by-the-lunisolar-gravitational-force-a-quantum-physical-approach
#16
Joachim Fisahn, Peter Barlow, Gerhard Dorda
Background and Aims: Roots of Arabidopsis thaliana exhibit a 24.8 h oscillation of elongation rate when grown under free-running conditions. This growth rhythm is synchronized with the time course of the local lunisolar tidal acceleration. The present study aims at a physiological/physical model to describe the interaction of weak gravitational fields with cellular water dynamics that mediate rhythmic root growth profiles. Methods: Fundamental physical laws are applied to model the water dynamics within single plant cells in an attempt to mimic the 24...
December 10, 2017: Annals of Botany
https://www.readbyqxmd.com/read/29236788/aflatoxin-and-viral-hepatitis-exposures-in-guatemala-molecular-biomarkers-reveal-a-unique-profile-of-risk-factors-in-a-region-of-high-liver-cancer-incidence
#17
Joshua W Smith, Maria F Kroker-Lobos, Mariana Lazo, Alvaro Rivera-Andrade, Patricia A Egner, Heiner Wedemeyer, Olga Torres, Neal D Freedman, Katherine A McGlynn, Eliseo Guallar, John D Groopman, Manuel Ramirez-Zea
Liver cancer is an emerging global health issue, with rising incidence in both the United States and the economically developing world. Although Guatemala experiences the highest rates of this disease in the Western hemisphere and a unique 1:1 distribution in men and women, few studies have focused on this population. Thus, we determined the prevalence and correlates of aflatoxin B1 (AFB1) exposure and hepatitis virus infection in Guatemalan adults. Healthy men and women aged ≥40 years (n = 461), residing in five departments of Guatemala, were enrolled in a cross-sectional study from May-October of 2016...
2017: PloS One
https://www.readbyqxmd.com/read/29236054/genome-wide-profiling-of-small-rnas-and-degradome-revealed-conserved-regulations-of-mirnas-on-auxin-responsive-genes-during-fruit-enlargement-in-peaches
#18
Mengya Shi, Xiao Hu, Yu Wei, Xu Hou, Xue Yuan, Jun Liu, Yueping Liu
Auxin has long been known as a critical phytohormone that regulates fruit development in plants. However, due to the lack of an enlarged ovary wall in the model plants Arabidopsis and rice, the molecular regulatory mechanisms of fruit division and enlargement remain unclear. In this study, we performed small RNA sequencing and degradome sequencing analyses to systematically explore post-transcriptional regulation in the mesocarp at the hard core stage following treatment of the peach (Prunus persica L.) fruit with the synthetic auxin α-naphthylacetic acid (NAA)...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29236047/hasc-and-dfat-multipotent-stem-cells-for-regenerative-medicine-a-comparison-of-their-potential-differentiation-in-vitro
#19
Marco Saler, Laura Caliogna, Laura Botta, Francesco Benazzo, Federica Riva, Giulia Gastaldi
Adipose tissue comprises both adipose and non-adipose cells such as mesenchymal stem cells. These cells show a surface antigenic profile similar to that of bone-marrow-derived MSC. The cells derived from the dedifferentiation of mature adipocytes (DFAT) are another cell population with characteristics of stemness. The aim of this study is to provide evidence of the stemness, proliferation, and differentiation of human adipose stem cells (hASC) and DFAT obtained from human subcutaneous AT and evaluate their potential use in regenerative medicine...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29235553/pro-inflammatory-gene-expression-profile-in-obese-adults-with-high-plasma-gip-levels
#20
J Góralska, U Raźny, A Polus, J Stancel-Możwiłło, M Chojnacka, A Gruca, A Zdzienicka, A Dembińska-Kieć, B Kieć-Wilk, B Solnica, M Malczewska-Malec
BACKGROUND: GIP provides a novel link between the immune system and the gut, although results from different experimental and observational studies are contradictory, ranging from anti-inflammatory, through neutral to pro-inflammatory action of GIP. Thus, the aim of this study was to analyze inflammatory pathways on the level of gene expression and circulating inflammatory markers in relation to plasma GIP level. SUBJECTS/METHODS: The study included 128 obese adults...
December 13, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
keyword
keyword
17809
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"