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https://www.readbyqxmd.com/read/29679761/expression-profiling-and-microbial-ligand-binding-analysis-of-high-mobility-group-box-1-hmgb1-in-turbot-scophthalmus-maximus-l
#1
Xin Cai, Chengbin Gao, Baofeng Su, Fenghua Tan, Ning Yang, Guodong Wang
High-mobility group box 1 (HMGB1), a highly conserved DNA-binding protein, was involved in nucleosome formation and transcriptional regulation, and can also act as an extracellular cytokine to trigger inflammation and immune responses. In this study, we identified a HMGB1 gene in turbot (Scophthalmus maximus L.). The full-length SaHMGB1 cDNA includes an open reading frame of 615 bp which encoded a 204 amino acid polypeptide with an estimated molecular mass of 23.19°kDa. SaHMGB1 was most closely related to several fish HMGB1 and shared 74...
April 18, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29679651/use-of-pcr-denaturing-gradient-gel-electrophoresis-for-the-discrimination-of-candida-species-isolated-from-natural-habitats
#2
Abd El-Latif Hesham, Vijai Kumar Gupta, Bhim Pratap Singh
Candida species are opportunistic microbes that cause chronic infections for a human being. Therefore, the exact identification of Candida species is extremely important for improved therapeutic strategy against these species. Identification based on conventional methods cannot differentiate between some of yeasts species, hence PCR based molecular techniques and sequencing could be an alternative tool for the yeasts identification. A quick molecular method based on the polymerase chain reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) was applied for distinguishing strains belonging to the Candida species...
April 18, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29679497/ewsr1-patz1-gene-fusion-may-define-a-new-glioneuronal-tumor-entity
#3
Aurore Siegfried, Audrey Rousseau, Claude-Alain Maurage, Sarah Pericart, Yvan Nicaise, Frederic Escudie, David Grand, Alix Delrieu, Anne Gomez-Brouchet, Sophie Le Guellec, Camille Franchet, Sergio Boetto, Matthieu Vinchon, Jean-Christophe Sol, Franck-Emmanuel Roux, Valerie Rigau, Anne-Isabelle Bertozzi, David Tw Jones, Dominique Figarella-Branger, Emmanuelle Uro-Coste
We investigated the challenging diagnostic case of a ventricular cystic glioneuronal tumor with papillary features, by RNA sequencing using the Illumina TruSight RNA Fusion panel. We did not retrieve the SLC44A1-PRKCA fusion gene specific for papillary glioneuronal tumor, but an EWSR1-PATZ1 fusion transcript. RT-PCR followed by Sanger sequencing confirmed the EWSR1-PATZ1 fusion. It matched with canonic EWSR1 fusion oncogene, juxtaposing the entire N terminal transcriptional activation domain of EWSR1 gene and the C terminal DNA binding domain of a transcription factor gene, PATZ1...
April 21, 2018: Brain Pathology
https://www.readbyqxmd.com/read/29679371/fitting-the-epigenome-into-the-picture-methylation-classification-for-paediatric-brain-tumours
#4
EDITORIAL
J A Cotter, A R Judkins
Meaningful classification of paediatric brain tumours has been a topic of revived discussion in the current era of molecular analysis. In recent years, we have seen the practice of diagnostic neuropathology not only incorporate routine biomarker interpretation (e.g. INI1/SMARCB1 and BRAF V600E immunohistochemistry), but also extend its reach into the molecular pathology laboratory. Next-generation sequencing, fusion analysis, and copy number profiling are now commonly-employed diagnostic tools, particularly in histologically challenging or clinically difficult cases...
April 20, 2018: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/29679203/a-window-into-extreme-longevity-the-circulating-metabolomic-signature-of-the-naked-mole-rat-a-mammal-that-shows-negligible-senescence
#5
Kaitlyn N Lewis, Nimrod D Rubinstein, Rochelle Buffenstein
Mouse-sized naked mole-rats (Heterocephalus glaber), unlike other mammals, do not conform to Gompertzian laws of age-related mortality; adults show no age-related change in mortality risk. Moreover, we observe negligible hallmarks of aging with well-maintained physiological and molecular functions, commonly altered with age in other species. We questioned whether naked mole-rats, living an order of magnitude longer than laboratory mice, exhibit different plasma metabolite profiles, which could then highlight novel mechanisms or targets involved in disease and longevity...
April 20, 2018: GeroScience
https://www.readbyqxmd.com/read/29679021/systematic-evaluation-of-rna-quality-microarray-data-reliability-and-pathway-analysis-in-fresh-fresh-frozen-and-formalin-fixed-paraffin-embedded-tissue-samples
#6
Isabella Wimmer, Anna R Tröscher, Florian Brunner, Stephen J Rubino, Christian G Bien, Howard L Weiner, Hans Lassmann, Jan Bauer
Formalin-fixed paraffin-embedded (FFPE) tissues are valuable resources commonly used in pathology. However, formalin fixation modifies nucleic acids challenging the isolation of high-quality RNA for genetic profiling. Here, we assessed feasibility and reliability of microarray studies analysing transcriptome data from fresh, fresh-frozen (FF) and FFPE tissues. We show that reproducible microarray data can be generated from only 2 ng FFPE-derived RNA. For RNA quality assessment, fragment size distribution (DV200) and qPCR proved most suitable...
April 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29678859/gsyellow-a-swiss-knife-tag-for-functional-protein-analysis-in-monocot-and-dicot-plants
#7
Nienke Besbrugge, Jelle Van Leene, Dominique Eeckhout, Bernard Cannoot, Shubhada R Kulkarni, Nancy De Winne, Geert Persiau, Eveline Van De Slijke, Michiel Bontinck, Stijn Aesaert, Francis Impens, Kris Gevaert, Daniel van Damme, Mieke Van Lijsebettens, Dirk Inze, Klaas Vandepoele, Hilde Nelissen, Geert De Jaeger
The ability to tag proteins has boosted the emergence of generic molecular methods for protein functional analysis. Fluorescent protein tags are used to visualize protein localization and affinity tags enable the mapping of molecular interactions by, for example, tandem affinity purification (TAP) or chromatin immunoprecipitation (ChIP). To apply these widely used molecular techniques on a single transgenic plant line, we developed a multifunctional TAP tag, named GSyellow, which combines the streptavidin-binding peptide (SBP) tag with citrine yellow fluorescent protein (cYFP)...
April 20, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29678793/characterisation-and-functional-comparison-of-single-crd-and-multidomain-containing-galectins-cggal-2-and-cggal-3-from-oyster-crassostrea-gigas
#8
Mengmeng Huang, Tao Zhou, Yuehong Wu, Hui Fei, Gaoyang Wang, Zhi Li, Yutong Lei, Qian Liu, Cong Sun, Zhengbing Lv, Xue-Wei Xu
Galectins are β-galactoside binding lectins that play crucial roles in innate immunity in vertebrates and invertebrates through their conserved carbohydrate-recognition domains (CRDs). In the present study, single- and four-CRD-containing galectins were identified in oyster Crassostrea gigas (designated CgGal-2 and CgGal-3). The open reading frames (ORFs) of CgGal-2 and CgGal-3 encode polypeptides of 200 and 555 amino acids, respectively. All CRDs of CgGal-3 include two consensus motifs essential for ligand-binding, and a novel motif is present in CgGal-2...
April 17, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29678717/a-tandem-mass-tag-tmt-proteomic-analysis-during-the-early-phase-of-experimental-pancreatitis-reveals-new-insights-in-the-disease-pathogenesis
#9
Violeta García-Hernández, Carmen Sánchez-Bernal, Domitille Schvartz, José J Calvo, Jean-Charles Sanchez, Jesús Sánchez-Yagüe
Changes in the protein expression occurring within the initiation phase of acute pancreatitis (AP) might be vital in the development of this complex disease. However, the exact mechanisms involved in the onset of AP remains elusive and most of our knowledge about the pathobiology of AP comes from animal models. We performed in a rat pancreatitic model a high-throughput shotgun proteomic profiling of the soluble and whole membrane fractions from the pancreas during the early phase of cerulein (Cer)-induced AP...
April 17, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29678535/design-synthesis-molecular-modelling-and-in-vitro-cytotoxicity-analysis-of-novel-carbamate-derivatives-as-inhibitors-of-monoacylglycerol-lipase
#10
Simone Lauria, Cristiana Perrotta, Silvana Casati, Ilaria Di Renzo, Roberta Ottria, Ivano Eberini, Luca Palazzolo, Chiara Parravicini, Pierangela Ciuffreda
Monoacylglycerol lipase (MAGL) has an essential role in the catabolic pathway of the endocannabinoid 2-arachidonoylglycerol, which makes it a potential target for highly specific inhibitors for the treatment of a number of diseases. We designed and synthesized a series of carbamate analogues of URB602. We evaluated their inhibitory activity toward human MAGL in vitro both in cell culture and lysates. The target compounds exhibited moderate to excellent inhibitory activity against MAGL. The most promising compound 2b showed good inhibitory activity with IC50 value of 4...
April 12, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29678299/molecular-epidemiology-of-klebsiella-pneumoniae-k1-and-k2-isolates-in-japan
#11
Sohei Harada, Yoshikazu Ishii, Tomoo Saga, Kotaro Aoki, Kazuhiro Tateda
Although severe infections caused by hypervirulent Klebsiella pneumoniae isolates, such as K1 isolates belonging to sequence type (ST) 23, have been a significant problem in Asian countries, epidemiology of these isolates in Japan remains unclear. We performed a nationwide molecular epidemiological study of K. pneumoniae K1 and K2 isolates in Japan. Of the 259K. pneumoniae isolates collected, 14 and 16 isolates were identified as capsular genotypes K1 and K2, respectively. All K1 isolates were ST23 or its closely related clones and showed high genetic similarity by pulsed-field gel electrophoresis (PFGE) and the DiversiLab system (DL)...
March 20, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29678161/an-unusually-high-frequency-of-scad-deficiency-caused-by-two-pathogenic-variants-in-the-acads-gene-and-its-relationship-to-the-ethnic-structure-in-slovakia
#12
Jana Lisyová, Ján Chandoga, Petra Jungová, Marcel Repiský, Mária Knapková, Martina Machková, Svetozár Dluholucký, Darina Behúlová, Jana Šaligová, Ľudmila Potočňáková, Miroslava Lysinová, Daniel Böhmer
BACKGROUND: Short-chain acyl-CoA dehydrogenase deficiency (SCADD) represents a rare autosomal recessive inborn metabolic disorder of mitochondrial β-oxidation of monocarboxylic acids. Clinical symptoms can vary from a severe life-threatening condition to an asymptomatic state, reported in the majority of cases. Since the expansion of newborn screenings, more than three hundred probands were admitted for molecular-genetic analysis, most selected because of elevated values of C4-acylcarnitine detected in newborn screenings in Slovakia...
April 20, 2018: BMC Medical Genetics
https://www.readbyqxmd.com/read/29678114/vertical-stratification-engineering-for-organic-bulk-heterojunction-devices
#13
Liqiang Huang, Gang Wang, Weihua Zhou, Boyi Fu, Xiaofang Cheng, Lifu Zhang, Zhibo Yuan, Sixing Xiong, Lin Zhang, Yuanpeng Xie, Andong Zhang, Youdi Zhang, Wei Ma, Weiwei Li, Yinhua Zhou, Elsa Reichmanis, Yiwang Chen
High efficiency organic solar cells (OSCs) can be produced through optimization of component molecular design, coupled with interfacial engineering and control of active layer morphology. However, vertical stratification of the bulk-heterojunction (BHJ), a spontaneous activity which occurs during the drying process, remains an intricate problem yet to be solved. Routes toward regulating the vertical separation profile and evaluating the effects on the final device should be explored to further enhance the performance of OSCs...
April 20, 2018: ACS Nano
https://www.readbyqxmd.com/read/29677477/systems-immunology-learning-the-rules-of-the-immune-system
#14
Alexandra-Chloé Villani, Siranush Sarkizova, Nir Hacohen
Given the many cell types and molecular components of the human immune system, along with vast variations across individuals, how should we go about developing causal and predictive explanations of immunity? A central strategy in human studies is to leverage natural variation to find relationships among variables, including DNA variants, epigenetic states, immune phenotypes, clinical descriptors, and others. Here, we focus on how natural variation is used to find patterns, infer principles, and develop predictive models for two areas: (a) immune cell activation-how single-cell profiling boosts our ability to discover immune cell types and states-and (b) antigen presentation and recognition-how models can be generated to predict presentation of antigens on MHC molecules and their detection by T cell receptors...
April 26, 2018: Annual Review of Immunology
https://www.readbyqxmd.com/read/29677135/molecular-evolution-and-expression-divergence-of-hmt-gene-family-in-plants
#15
Man Zhao, Peng Chen, Wenyi Wang, Fengjie Yuan, Danhua Zhu, Zhao Wang, Xiangxian Ying
Homocysteine methyltransferase (HMT) converts homocysteine to methionine using S -methylmethionine (SMM) or S -adenosylmethionine (SAM) as methyl donors in organisms, playing an important role in supplying methionine for the growth and the development of plants. To better understand the functions of the HMT genes in plants, we conducted a wide evolution and expression analysis of these genes. Reconstruction of the phylogenetic relationship showed that the HMT gene family was divided into Class 1 and Class 2...
April 20, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29676778/indirect-co-cultures-of-healthy-mesenchymal-stem-cells-restore-the-physiological-phenotypic-profile-of-psoriatic-mesenchymal-stem-cells
#16
A Campanati, M Orciani, G Sorgentoni, V Consales, M Mattioli Belmonte, R Di Primio, A Offidani
Psoriasis microenvironment, characterized by an imbalance between Th1/Th17 and Th2 cytokines, influences also mesenchymal stem cells (MSCs) phenotypic profile. MSCs from healthy donors (H-MSCs) can exert a strong paracrine effect by secreting active soluble factors, able to modulate the inflammation in the microenvironment. To evaluate the influence of H-MSCs on MSCs from psoriatic patients (PsO-MSCs), H-MSCs and PsO-MSCs were isolated and characterized. Indirect co-culture of H-MSCs with PsO-MSCs was performed; effects on proliferation and expression of cytokines linked to Th1/Th17 and Th2 pathways were assayed before and after co-culture...
April 20, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29676727/characterization-of-sccmec-type-iv-methicillin-resistant-staphylococcus-aureus-clones-increased-in-japanese-hospitals
#17
Hidemasa Nakaminami, Shunsuke Takadama, Ayumu Ito, Mariko Hasegawa, Chika Jono, Miyuki Noguchi, Manami Shoshi, Takeaki Wajima, Takeshi Fujii, Hiroshi Maruyama, Haruo Sakamoto, Yutaka Ito, Satsuki Okamoto, Yukiyoshi Masaki, Kayoko Tsuchiya, Susumu Nishinarita, Norihisa Noguchi
Recently, the prevalence of staphylococcal cassette chromosome mec (SCCmec) type IV isolates, which are the major community-acquired methicillin-resistant Staphylococcus aureus (MRSA), have increased in Japanese hospitals. The aim of this study was to elucidate the detailed molecular epidemiological features of the SCCmec type IV clones in Japanese hospitals. When 2589 MRSA isolated from four hospitals in Tokyo, Japan between 2010 and 2014 were analysed, the proportion of SCCmec type IV overtook that of type II, which was the major type of hospital-acquired MRSA in 2014...
April 20, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29676695/huge-heterogeneity-in-survival-in-a-subset-of-adult-patients-with-resected-wild-type-isocitrate-dehydrogenase-status-who-grade-ii-astrocytomas
#18
Gaëtan Poulen, Catherine Gozé, Valérie Rigau, Hugues Duffau
OBJECTIVE World Health Organization grade II gliomas are infiltrating tumors that inexorably progress to a higher grade of malignancy. However, the time to malignant transformation is quite unpredictable at the individual patient level. A wild-type isocitrate dehydrogenase (IDH-wt) molecular profile has been reported as a poor prognostic factor, with more rapid progression and a shorter survival compared with IDH-mutant tumors. Here, the oncological outcomes of a series of adult patients with IDH-wt, diffuse, WHO grade II astrocytomas (AII) who underwent resection without early adjuvant therapy were investigated...
April 20, 2018: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29676660/new-tailored-substituted%C3%A2-benzothiazole-schiff-base-cu-ii-zn-ii-antitumor-drug-entities-effect-of-substituents-on-dna-binding-profile-antimicrobial-and-cytotoxic-activity
#19
Siffeen Zehra, Mohammad Shavez Khan, Iqbal Ahmad, Farukh Arjmand
New tailored Cu(II) & Zn(II) metal-based antitumor drug entities were synthesized from substituted benzothiazole o‒vanillin Schiff base ligands. The complexes were thoroughly characterized by elemental analysis, spectroscopic studies {IR, 1 H & 13 C NMR, ESI-MS, EPR} and magnetic susceptibility measurements. The structure activity relationship (SAR) studies of benzothiazole Cu(II) & Zn(II) complexes having molecular formulas [C30 H22 CuN5 O7 S2 ], [C30 H20 Cl2 CuN5 O7 S2 ], [C30 H20 CuF2 N5 O7 S2 ], [C30 H22 N4 O4 S2 Zn], [C30 H20 Cl2 N4 O4 S2 Zn] and [C30 H20 F2 N5 O7 S2 Zn], with CT‒DNA were performed by employing absorption, emission titrations and hydrodynamic measurements...
April 20, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29676642/immunomethylomics-a-novel-cancer-risk-prediction-tool
#20
Karl T Kelsey, John K Wiencke
There is emerging evidence that the immune biology associated with lung and other solid tumors, as well as patient immune genetic traits, contributes to individual survival. At this time, dramatic advances in immunologic approaches to the study and management of human cancers are taking place, including lung and head and neck squamous cell carcinoma. However, major obstacles for therapies are the profound immune alterations in blood and in the tumor microenvironment that arise in tandem with the cancer. Although there is a significant current effort underway across the cancer research community to probe the tumor environment to uncover the dynamics of the immune response, little similar work is being done to understand the dynamics of immune alterations in peripheral blood, despite evidence showing the prognostic relevance of the neutrophil/lymphocyte ratio for these cancers...
April 2018: Annals of the American Thoracic Society
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