keyword
MENU ▼
Read by QxMD icon Read
search

Molecular profiling

keyword
https://www.readbyqxmd.com/read/28108917/quantitative-proteomics-analysis-reveals-the-tolerance-of-mirabilis-jalapa-l-to-petroleum-contamination
#1
Shuisen Chen, Hui Ma, Zhifu Guo, Yaping Feng, Jingwei Lin, Menghua Zhang, Ming Zhong
Petroleum is not only an important energy resource but is also a major soil pollutant. To gain better insight into the adaptability mechanism of Mirabilis jalapa to petroleum-contaminated soil, the protein profiles of M. jalapa root were investigated using label-free quantitative proteomics technique. After exposing to petroleum-contaminated soil for 24 h, 34 proteins significantly changed their protein abundance and most of the proteins increased in protein abundance (91.18%). Combined with gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses as well as data from previous studies, our results revealed that M...
January 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28108659/yamat-seq-an-efficient-method-for-high-throughput-sequencing-of-mature-transfer-rnas
#2
Megumi Shigematsu, Shozo Honda, Phillipe Loher, Aristeidis G Telonis, Isidore Rigoutsos, Yohei Kirino
Besides translation, transfer RNAs (tRNAs) play many non-canonical roles in various biological pathways and exhibit highly variable expression profiles. To unravel the emerging complexities of tRNA biology and molecular mechanisms underlying them, an efficient tRNA sequencing method is required. However, the rigid structure of tRNA has been presenting a challenge to the development of such methods. We report the development of Y-shaped Adapter-ligated MAture TRNA sequencing (YAMAT-seq), an efficient and convenient method for high-throughput sequencing of mature tRNAs...
January 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28108384/molecular-features-of-the-l-type-amino-acid-transporter-2-determine-different-import-and-export-profiles-for-thyroid-hormones-and-amino-acids
#3
Katrin M Hinz, Dominik Neef, Claudia Rutz, Jens Furkert, Josef Köhrle, Ralf Schülein, Gerd Krause
The L-type amino acid transporter 2 (LAT2) imports amino acids (AA) and also certain thyroid hormones (TH), e.g. 3,3'-T2 and T3, but not rT3 and T4. We utilized LAT2 mutations (Y130A, N133S, F242W) that increase 3,3'-T2 import and focus here on import and export capacity for AA, T4, T3, BCH and derivatives thereof to delineate molecular features. Transport studies and analysis of competitive inhibition of import by radiolabelled TH and AA were performed in Xenopus laevis oocytes. Only Y130A, a pocket widening mutation, enabled import for T4 and increased it for T3...
January 17, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28108350/molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-isolated-from-blood-in-rio-de-janeiro-displaying-susceptibility-profiles-to-non-%C3%AE-lactam-antibiotics
#4
Alexandra Vidal Pedinotti Zuma, Danielle Ferreira Lima, Ana Paula D'Alincourt Carvalho Assef, Elizabeth Andrade Marques, Robson Souza Leão
The distinction between healthcare-associated MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA) infections has become increasingly blurred. We assessed the molecular characterization and antimicrobial resistance profile for MRSA isolates from blood. Most of all (81.9%) isolates are related to known HA-MRSA and CA-MRSA epidemic lineages, such as, USA300, USA400, USA600, USA800 and USA1100. This is the first multicenter study in Rio de Janeiro.
December 21, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28108340/rna-seq-transcriptome-analysis-of-the-olive-flounder-paralichthys-olivaceus-kidney-response-to-vaccination-with-heat-inactivated-viral-hemorrhagic-septicemia-virus
#5
Jee Youn Hwang, Mun Gyeoung Kwon, Sung-Hee Jung, Myoung Ae Park, Dong-Wook Kim, Wang Sik Cho, Jeong Woo Park, Maeng-Hyun Son
Viral hemorrhagic septicemia (VHS) is a highly contagious disease of cultured flounder caused by VHS virus (VHSV). To develop effective VHSV vaccines, it is essential to understand the molecular mechanisms underlying the host's protective response against VHSV. The purpose of this study is to clarify which genes are involved in the protective response of olive flounder after VHSV vaccination. We first injected olive flounder intraperitoneally with 10(7) TCID50 heat-inactivated VHSV vaccine and evaluated the vaccine efficacy at 20 °C...
January 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28108297/the-leukotriene-b4-and-its-receptor-blt1-act-as-critical-drivers-of-neutrophil-recruitment-in-murine-bullous-pemphigoid-like-epidermolysis-bullosa-acquisita
#6
Tanya Sezin, Matthias Krajewski, Adam Wutkowski, Sadegh Mousavi, Lenche Chakievska, Katja Bieber, Ralf J Ludwig, Markus Dahlke, Dirk Rades, Franziska S Schulze, Enno Schmidt, Kathrin Kalies, Yask Gupta, Paul Schilf, Saleh M Ibrahim, Peter König, Dominik Schwudke, Detlef Zillikens, Christian D Sadik
Recruitment of neutrophils and eosinophils into the skin is a hallmark of pemphigoid diseases. The molecular cues regulating granulocyte recruitment into the skin as well as the individual contribution of neutrophils and eosinophils to pemphigoid diseases are, however, poorly understood. The lipid mediator leukotriene B4 (LTB4) is a potent granulocyte chemoattractant and abundant in the skin blister fluid of bullous pemphigoid (BP) patients, but its pathogenic significance is unknown. Using mouse models of (BP)-like epidermolysis bullosa acquisita (EBA) and of BP, we show that LTB4 and its receptor BLT1 act as critical drivers of neutrophil entry into the skin upon antibody deposition at the dermal-epidermal junction...
January 17, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28108277/carbon-monoxide-reverses-the-metabolic-adaptation-of-microglia-cells-to-an-inflammatory-stimulus
#7
Jayne Louise Wilson, Frédéric Bouillaud, Ana S Almeida, Helena L Vieira, Mohand Ouidir Ouidja, Jean-Luc Dubois-Randé, Roberta Foresti, Roberto Motterlini
Microglia fulfill important immunological functions in the brain by responding to pathological stresses and modulating their activities according to pro- or anti-inflammatory stimuli. Recent evidence indicates that changes in metabolism accompany the switch in microglia activation state, favoring glycolysis over oxidative phosphorylation when cells exhibit a pro-inflammatory phenotype. Carbon monoxide (CO), a byproduct of heme breakdown by heme oxygenase, exerts anti-inflammatory action and affects mitochondrial function in cells and tissues...
January 18, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28108062/chronic-vortioxetine-treatment-in-rodents-modulates-gene-expression-of-neurodevelopmental-and-plasticity-markers
#8
Jessica A Waller, Joseph A Tamm, Aicha Abdourahman, Alan L Pehrson, Yan Li, Manuel Cajina, Connie Sánchez
The multimodal antidepressant vortioxetine displays an antidepressant profile distinct from those of conventional selective serotonin reuptake inhibitors (SSRIs) and serotonin-norepinephrine reuptake inhibitors (SNRIs) and possesses cognitive-enhancing properties in preclinical and clinical studies. Recent studies have begun to investigate molecular mechanisms that may differentiate vortioxetine from other antidepressants. Acute studies in adult rats and chronic studies in a middle-aged mouse model reveal upregulation of several markers that play a central role in synaptic plasticity...
January 17, 2017: European Neuropsychopharmacology: the Journal of the European College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28107965/loopback-rolling-circle-amplification-for-ultrasensitive-detection-of-kras-gene
#9
Huo Xu, Dong Wu, Yifan Jiang, Rongbo Zhang, Qingzheng Wu, Yiyun Liu, Feng Li, Zai-Sheng Wu
Mutations in Kras gene may be used as a diagnostic marker and a target for treatment of the broad spectrum of human cancers. In this study, we developed a new class of amplification assay, double-hairpin molecular beacon (DHMB)-based cascade rolling circle amplification (RCA), for ultrasensitive and selective detection of Kras gene in a homogenous solution. Specifically, target DNA can hybridize with DHMB and activate cyclical target strand-displacement polymerization (CTDP) and nicking-mediated strand-displacement polymerization (NMDP)...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107645/a-cu-rious-ribosomal-profiling-pattern-leads-to-the-discovery-of-ribosomal-frameshifting-in-the-synthesis-of-a-copper-chaperone
#10
John F Atkins, Gary Loughran, Pavel V Baranov
In many bacteria, separate genes encode a copper binding chaperone and a copper efflux pump, but in some the chaperone encoding gene has been elusive. In this issue of Molecular Cell, Meydan et al. (2017) report that ribosomes translating the ORF that encodes the copper pump frequently frameshift and terminate to produce the copper chaperone.
January 19, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28107482/a-circulating-microrna-signature-predicts-age-based-development-of-lymphoma
#11
Afshin Beheshti, Charles Vanderburg, J Tyson McDonald, Charusheila Ramkumar, Tatenda Kadungure, Hong Zhang, Ronald B Gartenhaus, Andrew M Evens
Extensive epidemiological data have demonstrated an exponential rise in the incidence of non-Hodgkin lymphoma (NHL) that is associated with increasing age. The molecular etiology of this remains largely unknown, which impacts the effectiveness of treatment for patients. We proposed that age-dependent circulating microRNA (miRNA) signatures in the host influence diffuse large B cell lymphoma (DLBCL) development. Our objective was to examine tumor development in an age-based DLBCL system using an inventive systems biology approach...
2017: PloS One
https://www.readbyqxmd.com/read/28106823/integrated-microrna-mrna-profiling-identifies-oncostatin-m-as-a-marker-of-mesenchymal-like-er-negative-her2-negative-breast-cancer
#12
Giulia Bottai, Lixia Diao, Keith A Baggerly, Laura Paladini, Balázs Győrffy, Carlotta Raschioni, Lajos Pusztai, George A Calin, Libero Santarpia
MicroRNAs (miRNAs) simultaneously modulate different oncogenic networks, establishing a dynamic system of gene expression and pathway regulation. In this study, we analyzed global miRNA and messenger RNA (mRNA) expression profiles of 17 cell lines representing different molecular breast cancer subtypes. Spearman's rank correlation test was used to evaluate the correlation between miRNA and mRNA expression. Hierarchical clustering and pathway analysis were also performed. Publicly available gene expression profiles (n = 699) and tumor tissues (n = 80) were analyzed to assess the relevance of key miRNA-regulated pathways in human breast cancer...
January 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28106813/transcriptome-profiling-of-the-lungs-reveals-molecular-clock-genes-expression-changes-after-chronic-exposure-to-ambient-air-particles
#13
Pengcheng Song, Zhigang Li, Xiaoqian Li, Lixin Yang, Lulu Zhang, Nannan Li, Chen Guo, Shuyu Lu, Yongjie Wei
The symptoms of asthma, breathlessness, insomnia, etc. all have relevance to pulmonary rhythmic disturbances. Epidemiology and toxicology studies have demonstrated that exposure to ambient air particles can result in pulmonary dysfunction. However, there are no data directly supporting a link between air pollution and circadian rhythm disorder. In the present study, we found that breathing highly polluted air resulted in changes of the molecular clock genes expression in lung by transcriptome profiling analyses in a rodent model...
January 18, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28106290/returning-to-normal-assessing-transcriptome-recovery-over-time-in-male-rainbow-darter-etheostoma-caeruleum-liver-in-response-to-wastewater-treatment-plant-upgrades
#14
Patricija Marjan, Christopher J Martyniuk, Meghan L M Fuzzen, Deborah L MacLatchy, Mark E McMaster, Mark R Servos
The present study measured hepatic transcriptome responses in male rainbow darter (Etheostoma caeruleum) exposed to 2 municipal wastewater treatment plants (MWWTPs) (Kitchener and Waterloo) over 4 fall seasons (2011 - 2014) in the Grand River Ontario. The overall goal was to determine if upgrades at the Kitchener MWWTP (in 2012) resulted in transcriptome responses indicative of improved effluent quality. The number of differentially expressed probes in fish downstream of the Kitchener outfall (904-1,223), remained comparable to that downstream of Waterloo (767-3,867)...
January 20, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28106078/comparative-phosphoproteomics-reveals-an-important-role-of-mkk2-in-banana-musa-spp-cold-signal-network
#15
Jie Gao, Sheng Zhang, Wei-Di He, Xiu-Hong Shao, Chun-Yu Li, Yue-Rong Wei, Gui-Ming Deng, Rui-Bin Kuang, Chun-Hua Hu, Gan-Jun Yi, Qiao-Song Yang
Low temperature is one of the key environmental stresses, which greatly affects global banana production. However, little is known about the global phosphoproteomes in Musa spp. and their regulatory roles in response to cold stress. In this study, we conducted a comparative phosphoproteomic profiling of cold-sensitive Cavendish Banana and relatively cold tolerant Dajiao under cold stress. Phosphopeptide abundances of five phosphoproteins involved in MKK2 interaction network, including MKK2, HY5, CaSR, STN7 and kinesin-like protein, show a remarkable difference between Cavendish Banana and Dajiao in response to cold stress...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28106042/diverse-feather-shape-evolution-enabled-by-coupling-anisotropic-signalling-modules-with-self-organizing-branching-programme
#16
Ang Li, Seth Figueroa, Ting-Xin Jiang, Ping Wu, Randall Widelitz, Qing Nie, Cheng-Ming Chuong
Adaptation of feathered dinosaurs and Mesozoic birds to new ecological niches was potentiated by rapid diversification of feather vane shapes. The molecular mechanism driving this spectacular process remains unclear. Here, through morphology analysis, transcriptome profiling, functional perturbations and mathematical simulations, we find that mesenchyme-derived GDF10 and GREM1 are major controllers for the topologies of rachidial and barb generative zones (setting vane boundaries), respectively, by tuning the periodic-branching programme of epithelial progenitors...
January 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28105957/identification-of-nuclear-genes-controlling-chlorophyll-synthesis-in%C3%A2-barley-by-rna-seq
#17
Nickolay A Shmakov, Gennadiy V Vasiliev, Natalya V Shatskaya, Alexey V Doroshkov, Elena I Gordeeva, Dmitry A Afonnikov, Elena K Khlestkina
BACKGROUND: Albinism in plants is characterized by lack of chlorophyll and results in photosynthesis impairment, abnormal plant development and premature death. These abnormalities are frequently encountered in interspecific crosses and tissue culture experiments. Analysis of albino mutant phenotypes with full or partial chlorophyll deficiency can shed light on genetic determinants and molecular mechanisms of albinism. Here we report analysis of RNA-seq transcription profiling of barley (Hordeum vulgare L...
November 16, 2016: BMC Plant Biology
https://www.readbyqxmd.com/read/28105955/small-rna-profiles-in-soybean-primary-root-tips-under-water-deficit
#18
Yun Zheng, Vandana Hivrale, Xiaotuo Zhang, Babu Valliyodan, Christine Lelandais-Brière, Andrew D Farmer, Gregory D May, Martin Crespi, Henry T Nguyen, Ramanjulu Sunkar
BACKGROUND: Soybean (Glycine max) production is significantly hampered by frequent droughts in many regions of the world including the United States. Identifying microRNA (miRNA)-controlled posttranscriptional gene regulation under drought will enhance our understanding of molecular basis of drought tolerance in this important cash crop. Indeed, miRNA profiles in soybean exposed to drought were studied but not from the primary root tips, which is not only a main zone of water uptake but also critical for water stress sensing and signaling...
December 5, 2016: BMC Systems Biology
https://www.readbyqxmd.com/read/28105926/the-gene-expression-profile-of-renal-medulla-in-isiah-rats-with-inherited-stress-induced-arterial-hypertension
#19
Marina A Ryazanova, Larisa A Fedoseeva, Nikita I Ershov, Vadim M Efimov, Arcady L Markel, Olga E Redina
BACKGROUND: The changes in the renal function leading to a reduction of medullary blood flow can have a great impact on sodium and water homeostasis and on the long-term control of arterial blood pressure. The RNA-Seq approach was used for transcriptome profiling of the renal medulla from hypertensive ISIAH and normotensive WAG rats to uncover the genetic basis of the changes underlying the renal medulla function in the ISIAH rats being a model of the stress-sensitive arterial hypertension and to reveal the genes which possibly may contribute to the alterations in medullary blood flow...
December 22, 2016: BMC Genetics
https://www.readbyqxmd.com/read/28105924/molecular-determinants-of-the-adrenal-gland-functioning-related-to-stress-sensitive-hypertension-in-isiah-rats
#20
Larisa A Fedoseeva, Leonid O Klimov, Nikita I Ershov, Yury V Alexandrovich, Vadim M Efimov, Arcady L Markel, Olga E Redina
BACKGROUND: The adrenals are known as an important link in pathogenesis of arterial hypertensive disease. The study was directed to the adrenal transcriptome analysis in ISIAH rats with stress-sensitive arterial hypertension and predominant involvement in pathogenesis of the hypothalamic-pituitary-adrenal and sympathoadrenal systems. RESULTS: The RNA-Seq approach was used to perform the comparative adrenal transcriptome profiling in hypertensive ISIAH and normotensive WAG rats...
December 28, 2016: BMC Genomics
keyword
keyword
17809
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"