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https://www.readbyqxmd.com/read/28089450/a-structural-model-for-vinculin-insertion-into-pip2-containing-membranes-and-the-effect-of-insertion-on-vinculin-activation-and-localization
#1
Peter M Thompson, Srinivas Ramachandran, Lindsay B Case, Caitlin E Tolbert, Arpit Tandon, Mihir Pershad, Nikolay V Dokholyan, Clare M Waterman, Sharon L Campbell
Vinculin, a scaffolding protein that localizes to focal adhesions (FAs) and adherens junctions, links the actin cytoskeleton to the adhesive super-structure. While vinculin binds to a number of cytoskeletal proteins, it can also associate with phosphatidylinositol 4,5-bisphosphate (PIP2) to drive membrane association. To generate a structural model for PIP2-dependent interaction of vinculin with the lipid bilayer, we conducted lipid-association, nuclear magnetic resonance, and computational modeling experiments...
December 31, 2016: Structure
https://www.readbyqxmd.com/read/28088458/non-thermal-atmospheric-pressure-plasma-functionalized-dental-implant-for-enhancement-of-bacterial-resistance-and-osseointegration
#2
Jung-Hwan Lee, Won-Seok Jeong, Seog-Jin Seo, Hae-Won Kim, Kyoung-Nam Kim, Eun-Ha Choi, Kwang-Mahn Kim
OBJECTIVE: Even though roughened titanium (Ti) and Ti alloys have been clinically used as dental implant, they encourage bacterial adhesion, leading to failure of the initial stability. Here, the non-thermal atmospheric pressure plasma jet (NTAPPJ) functionalized Ti and Ti alloy were investigated to promote cellular activities but inhibit the initial attachment of the adherent pioneer bacterium, Streptococcus sanguinis, without topographical changes. METHODS: After the produced radicals from NTAPPJ were characterized, bacterial adhesion to specimens was assessed by PrestoBlue assay and live-dead staining with or without the NTAPPJ functionalizing...
January 11, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28076902/decellularized-extracellular-matrix-of-human-umbilical-vein-endothelial-cells-promotes-endothelial-differentiation-of-stem-cells-from-exfoliated-deciduous-teeth
#3
Ting Gong, Boon Chin Heng, Jianguang Xu, Shaoyue Zhu, Changyong Yuan, Edward Chin Man Lo, Chengfei Zhang
Dental stem cells can serve as a potential source of functional endothelial cells (ECs) for tissue engineering applications, but the endothelial-lineage differentiation efficiency is rather low even with growth factors and mechanical stimuli, which greatly limits their clinical applications. This is partly due to the deficiency of standard two-dimensional (2-D) culture systems, which is unable to recapitulate the three-dimensional (3-D) in vivo milieu that is rich in extracellular matrix. Hence we extracted decellularized extracellular matrix from human umbilical vein endothelial cells (HUVECs-DECM) to provide a bioactive substratum conducive to the endothelial differentiation of dental stem cells...
January 11, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28068967/enterolactone-alters-fak-src-signaling-and-suppresses-migration-and-invasion-of-lung-cancer-cell-lines
#4
Shireen Chikara, Kaitlin Lindsey, Pawel Borowicz, Melpo Christofidou-Solomidou, Katie M Reindl
BACKGROUND: Systemic toxicity of chemotherapeutic agents and the challenges associated with targeting metastatic tumors are limiting factors for current lung cancer therapeutic approaches. To address these issues, plant-derived bioactive components have been investigated for their anti-cancer properties because many of these agents are non-toxic to healthy tissues. Enterolactone (EL) is a flaxseed-derived mammalian lignan that has demonstrated anti-migratory properties for various cancers, but EL has not been investigated in the context of lung cancer, and its anticancer mechanisms are ill-defined...
January 9, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28063985/revealing-the-three-dimensional-architecture-of-focal-adhesion-components-to-explain-ca-2-mediated-turnover-of-focal-adhesions
#5
Shu-Jing Chang, Ying-Chi Chen, Chi-Hsun Yang, Soon-Cen Huang, Ho-Kai Huang, Chun-Chun Li, Hans I-Chen Harn, Wen-Tai Chiu
BACKGROUND: Focal adhesions (FAs) are large, dynamic protein complexes located close to the plasma membrane, which serve as the mechanical linkages and a biochemical signaling hub of cells. The coordinated and dynamic regulation of focal adhesion is required for cell migration. Degradation, or turnover, of FAs is a major event at the trailing edge of a migratory cell, and is mediated by Ca(2+)/calpain-dependent proteolysis and disassembly. Here, we investigated how Ca(2+) influx induces cascades of FA turnover in living cells...
January 5, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28062282/coupling-of-vinculin-to-f-actin-demands-syndecan-4-proteoglycan
#6
R P Cavalheiro, M A Lima, T R Jarrouge-Bouças, G M Viana, C C Lopes, V J Coulson-Thomas, J L Dreyfuss, E A Yates, I L S Tersariol, H B Nader
Syndecans are heparan sulfate proteoglycans characterized as transmembrane receptors that act cooperatively with the cell surface and extracellular matrix proteins. Syn4 knockdown was performed in order to address its role in endothelial cells (EC) behavior. Normal EC and shRNA-Syn4-EC cells were studied comparatively using complementary confocal, super-resolution and non-linear microscopic techniques. Confocal and super-resolution microscopy revealed that Syn4 knockdown alters the level and arrangement of essential proteins for focal adhesion, evidenced by the decoupling of vinculin from F-actin filaments...
January 3, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28029098/mesenchymal-stem-cells-cultured-on-magnetic-nanowire-substrates
#7
Jose E Perez, Timothy Ravasi, Jürgen Kosel
Stem cells have been shown to respond to extracellular mechanical stimuli by regulating their fate through the activation of specific signaling pathways. In this work, an array of iron nanowires (NWs) aligned perpendicularly to the surface was fabricated by pulsed electrodepositon in porous alumina templates followed by a partial removal of the alumina to reveal 2-3 μm of the NWs. This resulted in alumina substrates with densely arranged NWs of 33 nm in diameter separated by 100 nm. The substrates were characterized by scanning electron microscopy (SEM) energy dispersive x-ray analysis and vibrating sample magnetometer...
February 3, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28027315/non-human-primate-schlafen11-inhibits-production-of-both-host-and-viral-proteins
#8
Alex C Stabell, John Hawkins, Manqing Li, Xia Gao, Michael David, William H Press, Sara L Sawyer
Schlafen11 (encoded by the SLFN11 gene) has been shown to inhibit the accumulation of HIV-1 proteins. We show that the SLFN11 gene is under positive selection in simian primates and is species-specific in its activity against HIV-1. The activity of human Schlafen11 is relatively weak compared to that of some other primate versions of this protein, with the versions encoded by chimpanzee, orangutan, gibbon, and marmoset being particularly potent inhibitors of HIV-1 protein production. Interestingly, we find that Schlafen11 is functional in the absence of infection and reduces protein production from certain non-viral (GFP) and even host (Vinculin and GAPDH) transcripts...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/28026000/low-magnitude-high-frequency-vibration-promotes-the-adhesion-and-the-osteogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells-cultured-on-a-hydroxyapatite-coated-surface-the-direct-role-of-wnt-%C3%AE-catenin-signaling-pathway-activation
#9
Bailing Chen, Tao Lin, Xiaoxi Yang, Yiqiang Li, Denghui Xie, Wenhui Zheng, Haowen Cui, Weimin Deng, Xin Tan
The positive effect of low-magnitude, high‑frequency (LMHF) vibration on implant osseointegration has been demonstrated; however, the underlying cellular and molecular mechanisms remain unknown. The aim of this study was to explore the effect of LMHF vibration on the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) cultured on hydroxyapatite (HA)-coated surfaces in an in vitro model as well as to elucidate the molecular mechanism responsible for the effects of LMHF vibration on osteogenesis...
November 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28024610/alkalescent-nanotube-films-on-a-titanium-based-implant-a-novel-approach-to-enhance-biocompatibility
#10
Yanxian Zhang, Chaofang Dong, Sefei Yang, Junsheng Wu, Kui Xiao, Yunhua Huang, Xiaogang Li
The interfacial pH value has a marked effect on cell viability because the pro-mineralization activity of osteoblasts increases at alkaline extracellular pH, whereas the pro-resorptive activity of osteoclasts increases under more acidic conditions. To obtain the more favorable alkaline interface, we developed a novel nanotube layer that was incorporated with magnesium oxide on a titanium implant substrate (MgO/NT/Ti) via ethylenediamine tetraacetic acid (EDTA) chelation. This facile immersion-annealing process successfully created a homogeneous magnesium oxide layer with sustained release kinetics and superior hydrophilicity according to the surface characterization and microenvironment measurement...
March 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28008179/resolving-the-cadherin-f-actin-connection
#11
Mitchell K L Han, Johan de Rooij
Cadherin adhesion complexes have recently emerged as sensors of tissue tension that regulate key developmental processes. Super-resolution microscopy experiments now unravel the spatial organization of the interface between cadherins and the actin cytoskeleton and reveal how vinculin, a central component in cadherin mechanotransduction, is regulated by mechanical and biochemical signals.
December 23, 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/28000113/adhesion-and-differentiation-of-saos-2-osteoblast-like-cells-on-chromium-doped-diamond-like-carbon-coatings
#12
Elena Filova, Marta Vandrovcova, Miroslav Jelinek, Josef Zemek, Jana Houdkova, Jan Remsa, Tomas Kocourek, Lubica Stankova, Lucie Bacakova
Diamond-like carbon (DLC) thin films are promising for use in coating orthopaedic, dental and cardiovascular implants. The problem of DLC layers lies in their weak layer adhesion to metal implants. Chromium is used as a dopant for improving the adhesion of DLC films. Cr-DLC layers were prepared by a hybrid technology, using a combination of pulsed laser deposition (PLD) from a graphite target and magnetron sputtering. Depending on the deposition conditions, the concentration of Cr in the DLC layers moved from zero to 10...
January 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27999452/microrna-regulation-of-endothelial-junction-proteins-and-clinical-consequence
#13
REVIEW
Yugang Zhuang, Hu Peng, Victoria Mastej, Weiguo Chen
Cellular junctions play a critical role in structural connection and signal communication between cells in various tissues. Although there are structural and functional varieties, cellular junctions include tight junctions, adherens junctions, focal adhesion junctions, and tissue specific junctions such as PECAM-1 junctions in endothelial cells (EC), desmosomes in epithelial cells, and hemidesmosomes in EC. Cellular junction dysfunction and deterioration are indicative of clinical diseases. MicroRNAs (miRNA) are ~20 nucleotide, noncoding RNAs that play an important role in posttranscriptional regulation for almost all genes...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27998102/three-dimensional-stiff-graphene-scaffold-on-neural-stem-cells-behavior
#14
Qinqin Ma, Lingyan Yang, Ziyun Jiang, Qin Song, Miao Xiao, Dong Zhang, Xun Ma, Tieqiao Wen, Guosheng Cheng
Physical cues of the scaffolds, elasticity, and stiffness significantly guide adhesion, proliferation, and differentiation of stem cells. In addressable microenvironments constructed by three-dimensional graphene foams (3D-GFs), neural stem cells (NSCs) interact with and respond to the structural geometry and mechanical properties of porous scaffolds. Our studies aim to investigate NSC behavior on the various stiffness of 3D-GFs. Two kinds of 3D-GFs scaffolds present soft and stiff properties with elasticity moduli of 30 and 64 kPa, respectively...
December 21, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27992423/angiotensin-converting-enzyme-regulates-cell-proliferation-and-migration
#15
Erika Costa de Alvarenga, Matheus de Castro Fonseca, Clarissa Coelho Carvalho, Rodrigo Machado Florentino, Andressa França, Eveline Matias, Paola Bianchi Guimarães, Carolina Batista, Valder Freire, Adriana Karaoglanovic Carmona, João Bosco Pesquero, Ana Maria de Paula, Giselle Foureaux, Maria de Fatima Leite
BACKGROUND: The angiotensin-I converting enzyme (ACE) plays a central role in the renin-angiotensin system, acting by converting the hormone angiotensin-I to the active peptide angiotensin-II (Ang-II). More recently, ACE was shown to act as a receptor for Ang-II, and its expression level was demonstrated to be higher in melanoma cells compared to their normal counterparts. However, the function that ACE plays as an Ang-II receptor in melanoma cells has not been defined yet. AIM: Therefore, our aim was to examine the role of ACE in tumor cell proliferation and migration...
2016: PloS One
https://www.readbyqxmd.com/read/27992406/nanoscale-architecture-of-cadherin-based-cell%C3%A2-adhesions
#16
Cristina Bertocchi, Yilin Wang, Andrea Ravasio, Yusuke Hara, Yao Wu, Talgat Sailov, Michelle A Baird, Michael W Davidson, Ronen Zaidel-Bar, Yusuke Toyama, Benoit Ladoux, Rene-Marc Mege, Pakorn Kanchanawong
Multicellularity in animals requires dynamic maintenance of cell-cell contacts. Intercellularly ligated cadherins recruit numerous proteins to form supramolecular complexes that connect with the actin cytoskeleton and support force transmission. However, the molecular organization within such structures remains unknown. Here we mapped protein organization in cadherin-based adhesions by super-resolution microscopy, revealing a multi-compartment nanoscale architecture, with the plasma-membrane-proximal cadherin-catenin compartment segregated from the actin cytoskeletal compartment, bridged by an interface zone containing vinculin...
January 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/27990827/coordination-between-intra-and-extracellular-forces-regulates-focal-adhesion-dynamics
#17
Bibhu Ranjan Sarangi, Mukund Gupta, Bryant L Doss, Nicolas Tissot, France Lam, René-Marc Mège, Nicolas Borghi, Benoît Ladoux
Focal adhesions (FAs) are important mediators of cell-substrate interactions. One of their key functions is the transmission of forces between the intracellular acto-myosin network and the substrate. However, the relationships between cell traction forces, FA architecture, and molecular forces within FAs are poorly understood. Here, by combining Förster resonance energy transfer (FRET)-based molecular force biosensors with micropillar-based traction force sensors and high-resolution fluorescence microscopy, we simultaneously map molecular tension across vinculin, a key protein in FAs, and traction forces at FAs...
December 23, 2016: Nano Letters
https://www.readbyqxmd.com/read/27987338/effect-of-interleukin-1%C3%AE-and-lipoxin-a4-in-human-endometriotic-stromal-cells-proteomic-analysis
#18
Rong-Feng Wu, Hui-Ming Yang, Wei-Dong Zhou, Li-Rong Zhang, Jian-Bing Bai, Dian-Chao Lin, Tai-Wei Ng, Song-Juan Dai, Qiong-Hua Chen, Qing-Xi Chen
AIM: Lipoxin A4 (LXA4 ) can function as an endogenous 'breaking signal' in inflammation and plays an important role in the progression of endometriosis. The proteome responses to interleukin-1β (IL-1β) or LXA4 in human endometriotic stromal cells (ESC) are not well understood. METHODS: In this study, primary ESC were cultured from ovarian endometriosis tissue. Three groups were established: the control group; the IL-1β stimulation group; and the IL-1β and LXA4 incubation group...
December 17, 2016: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/27980057/%C3%AE-catenin-and-vinculin-in-dorsal-closure
#19
(no author information available yet)
No abstract text is available yet for this article.
December 15, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27956359/chemical-group-dependent-plasma-polymerisation-preferentially-directs-adipose-stem-cell-differentiation-towards-osteogenic-or-chondrogenic-lineages
#20
M F Griffin, A Ibrahim, A M Seifalian, P E M Butler, D M Kalaskar, P Ferretti
: Human adipose derived stem cells (ADSCs) are being explored for the repair of craniofacial defects due to their multi-differentiation potential and ease of isolation and expansion. Crucial to using ADSCs for craniofacial repair is the availability of materials with appropriate biomechanical properties that can support their differentiation into bone and cartilage. We tested the hypothesis that different modifications of chemical groups on the surface of a nanocomposite polymer could increase human ADSC adhesion and selectively enhance their osteogenic and chondrogenic differentiation...
December 9, 2016: Acta Biomaterialia
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