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Amniotic fluid stem cells

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https://www.readbyqxmd.com/read/28935121/treatment-of-acute-ocular-chemical-burns
#1
REVIEW
Namrata Sharma, Manpreet Kaur, Tushar Agarwal, Virender S Sangwan, Rasik B Vajpayee
Ocular chemical burns are an ophthalmic emergency and are responsible for 11.5% to 22.1% of ocular injuries. Immediate copious irrigation is universally recommended in acute ocular burns to remove the offending agent and minimize damage. Conventional medical therapy consists of the use of agents that promote epithelialization, minimize inflammation, and prevent cicatricial complications. Biological fluids such as autologous serum, umbilical cord blood serum, platelet-rich plasma, and amniotic membrane suspension are a rich source of growth factors and promote healing when used as adjuncts to conventional therapy...
September 18, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/28928772/tissue-engineering-to-repair-diaphragmatic-defect-in-a-rat-model
#2
G P Liao, Y Choi, K Vojnits, H Xue, K Aroom, F Meng, H Y Pan, R A Hetz, C J Corkins, T G Hughes, F Triolo, A Johnson, Kenneth J Moise, K P Lally, C S Cox, Y Li
Tissue engineering is an emerging strategy for repairing damaged tissues or organs. The current study explored using decellularized rat diaphragm scaffolds combined with human amniotic fluid-derived multipotent stromal cells (hAFMSC) to provide a scaffold, stem cell construct that would allow structural barrier function during tissue ingrowth/regeneration. We created an innovative cell infusion system that allowed hAFMSC to embed into scaffolds and then implanted the composite tissues into rats with surgically created left-sided diaphragmatic defects...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28928383/human-stem-cells-alter-the-invasive-properties-of-somatic-cells-via-paracrine-activation-of-mtorc1
#3
Margit Rosner, Ha Thi Thanh Pham, Richard Moriggl, Markus Hengstschläger
Controlled invasion is essential during many physiological processes, whereas its deregulation is a hallmark of cancer. Here we demonstrate that embryonic, induced pluripotent and amniotic fluid stem cells share the property to induce the invasion of primary somatic cells of various origins through insulin-like growth factor I (IGF-I)- or II (IGF-II)-mediated paracrine activation of mechanistic target of rapamycin complex 1 (mTORC1). We propose a model in which downstream of mTORC1 this stem cell-induced invasion is mediated by hypoxia-inducible factor 1-alpha (HIF-1α)-regulated matrix metalloproteinases...
September 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28915235/transamniotic-stem-cell-therapy-trascet-a-novel-strategy-for-the-prenatal-management-of-congenital-anomalies
#4
REVIEW
Dario O Fauza
Transamniotic Stem Cell Therapy, or TRASCET, is an emerging therapeutic concept for the management of congenital anomalies based on the augmentation of the biological role of select populations of stem cells that already occur in the amniotic fluid, for targeted therapeutic benefit. Amniotic fluid-derived mesenchymal stem cells (afMSCs) play a central role in the enhanced ability of the fetus to repair tissue damage. This germane recent finding constitutes the biological foundation for the use of afMSCs in TRASCET...
September 15, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28854517/the-effect-of-meconium-exposure-on%C3%A2-the%C3%A2-expression-and-differentiation-of%C3%A2-amniotic-fluid-mesenchymal-stem-cells
#5
T J Jensen, J E Shui, C M Finck
BACKGROUND: The goal of this study was to determine if exposure to meconium would alter the phenotype of amniotic fluid mesenchymal stem cells (AF-MSCs) and the ability of these cells to be differentiated into distal airway type cells. METHODS: Meconium was collected, lyophilized and resuspended in PBS at 3 different concentrations (high, medium, and low). AF-MSCs were cultured in the presence of this meconium suspension for 8 hours and then analyzed for changes in gene expression...
August 23, 2017: Journal of Neonatal-perinatal Medicine
https://www.readbyqxmd.com/read/28842991/the-amniotic-fluid-as-a-source-of-mesenchymal-stem-cells-with-lung-specific-characteristics
#6
Flore Lesage, Silvia Zia, Julio Jiménez, Jan Deprest, Jaan Toelen
The amniotic fluid is a clinically accessible source of mesenchymal stem cells (AF-MSC) during gestation, which enables autologous cellular therapy for perinatal disorders. The origin of AF-MSC remains elusive: renal and neuronal progenitors have been isolated from the AF-MSC pool, yet no cells with pulmonary characteristics. We analyzed gene expression of pulmonary and renal markers of 212 clonal lines of AF-MSC isolated from amniocentesis samples. AF-MSC were cultured on dishes coated with extracellular matrix (ECM) proteins from decellularized fetal rabbit lungs...
August 26, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28841906/term-amniotic-fluid-an-unexploited-reserve-of-mesenchymal-stromal-cells-for-reprogramming-and-potential-cell-therapy-applications
#7
Roksana Moraghebi, Agnete Kirkeby, Patricia Chaves, Roger E Rönn, Ewa Sitnicka, Malin Parmar, Marcus Larsson, Andreas Herbst, Niels-Bjarne Woods
BACKGROUND: Mesenchymal stromal cells (MSCs) are currently being evaluated in numerous pre-clinical and clinical cell-based therapy studies. Furthermore, there is an increasing interest in exploring alternative uses of these cells in disease modelling, pharmaceutical screening, and regenerative medicine by applying reprogramming technologies. However, the limited availability of MSCs from various sources restricts their use. Term amniotic fluid has been proposed as an alternative source of MSCs...
August 25, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28835139/generation-of-induced-pluripotent-stem-cells-from-amniotic-fluid-cells-of-a-fetus-with-hb-bart-s-disease
#8
Jin-Mei Yan, Dong-Zhi Li
Induced pluripotent stem cells (iPSCs) derived from diseased patients behave as a powerful tool for biomedical research and may provide a source for replacement therapies. In this study, we generated iPSCs from amniotic fluid cells of a fetus with Hb Bart's (γ4) disease (- -/- -). The established iPSCs showed pluripotency similar to that of human embryonic stem cells. They were able to differentiate into various somatic cell types and maintained normal karyotypes after long periods of culture in vitro. The patient-specific iPSCs offer a valuable model for advancing α-thalassemia (α-thal) research and early treatment of the affected fetuses...
August 24, 2017: Hemoglobin
https://www.readbyqxmd.com/read/28752425/minimal-modulation-of-the-host-immune-response-to-sis-matrix-implants-by-mesenchymal-stem-cells-from-the-amniotic-fluid
#9
F Lesage, S Pranpanus, F M Bosisio, M Jacobs, S Ospitalieri, J Toelen, J Deprest
PURPOSE: Surgical restoration of soft tissue defects often requires implantable devices. The clinical outcome of the surgery is determined by the properties inherent to the used matrix. Mesenchymal stem cells (MSC) modulate the immune processes after in vivo transplantation and their addition to matrices is associated with constructive remodeling. Herein we evaluate the potential of MSC derived from the amniotic fluid (AF-MSC), an interesting MSC source for cell therapeutic applications in the perinatal period, for immune modulation when added to a biomaterial...
July 27, 2017: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/28747757/tgf%C3%AE-induced-osteogenic-potential-of-human-amniotic-fluid-stem-cells-via-cd73-generated-adenosine-production
#10
Kwan-Leong Hau, Anna Maria Ranzoni, Filipa Vlahova, Kate Hawkins, Paolo De Coppi, Anna L David, Pascale V Guillot
The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28708036/amniotic-fluid-cells-show-higher-pluripotency-related-gene-expression-than-allantoic-fluid-cells
#11
Debora Kehl, Melanie Generali, Sabrina Görtz, Diego Geering, Jaroslav Slamecka, Simon P Hoerstrup, Ulrich Bleul, Benedikt Weber
Amniotic fluid represents an abundant source of multipotent stem cells, referred as broadly multipotent given their differentiation potential and expression of pluripotency-related genes. However, the origin of this broadly multipotent cellular fraction is not fully understood. Several sources have been proposed so far, including embryonic and extraembryonic tissues. In this regard, the ovine developmental model uniquely allows for direct comparison of fetal fluid-derived cells from two separate fetal fluid cavities, the allantois and the amnion, over the entire duration of gestation...
August 14, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28679310/protein-and-molecular-characterization-of-a-clinically-compliant-amniotic-fluid-stem-cell-derived-extracellular-vesicle-fraction-capable-of-accelerating-muscle-regeneration-through-enhancement-of-angiogenesis
#12
Ben Mellows, Robert Mitchell, Manuela Antonioli, Oliver Kretz, David Chambers, Marie-Theres Zeuner, Bernd Denecke, Luca Musante, Durrgah L Ramachandra, Florence Debacq-Chainiaux, Harry Holthofer, Barbara Joch, Steve Ray, Darius Widera, Anna L David, Tobias B Huber, Joern Dengjel, Paolo De Coppi, Ketan Patel
The secretome of human amniotic fluid stem cells (AFSCs) has great potential as a therapeutic agent in regenerative medicine. However, it must be produced in a clinically compliant manner before it can be used in humans. In this study, we developed a means of producing a biologically active secretome from AFSCs that is free of all exogenous molecules. We demonstrate that the full secretome is capable of promoting stem cell proliferation, migration, and protection of cells against senescence. Furthermore, it has significant anti-inflammatory properties...
September 15, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28675062/lung-regeneration-using-amniotic-fluid-mesenchymal-stem-cells
#13
Alireza Azargoon, Babak Negahdari
Respiratory diseases, such as chronic obstructive pulmonary disease (COPD), pulmonary hypertension and lung fibrosis, are yet a major challenge in the world and they result in irreversible structural lung damage. Lung transplantation as the only therapeutic option face some major challenges like graft rejection and cancer, arising as a result of immunosuppression. A low survival rate faced by lung transplantation patients is presently limited to approximately 5 years. Lungs shortage therefore calls for a mechanism that would increase the availability of suitable organs for transplantation...
July 4, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28653478/different-rna-and-protein-expression-of-surface-markers-in-rabbit-amniotic-fluid-derived-mesenchymal-stem-cells
#14
Michal Kovac, Jaromir Vasicek, Barbora Kulikova, Miroslav Bauer, Jozef Curlej, Andrej Balazi, Peter Chrenek
Over the years, there has been much confusion in defining molecular markers of mesenchymal stem cells (MSCs) for other than human species due to a lack of species-specific antibodies. Therefore, the aim of our study was to define rabbit amniotic fluid-derived mesenchymal stem cells (rAF-MSCs) and to reflect upon the current identification of AF-MSCs by providing a summary of detected surface markers in different species. The expression of rAF-MSC surface markers was analyzed at the protein and mRNA level. Flow cytometry analyses showed that rAF-MSCs were positive for CD29 and CD44, low positive for CD90, but negative for CD73, CD105 and CD166...
June 27, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28629288/p53-mutation-and-epigenetic-imprinted-igf2-h19-gene-analysis-in-mesenchymal-stem-cells-derived-from-amniotic-fluid-amnion-endometrium-and-wharton-s-jelly
#15
Tatsanee Phermthai, Puttachart Pokathikorn, Suparat Wichitwiengrat, Sasiprapa Thongbopit, Kittima Tungprasertpol, Suphakde Julavijitphong
Mesenchymal stem cells (MSC) are promising cells for medical therapy. In in vitro expansion, MSC can give rise to progeny with genomic and epigenomic alterations, resulting in senescence, loss of terminal differentiation, and transformation to cancer. However, MSC genome protects its genetic instability by a guardian function of the P53 tumor suppressor gene and epigenetic balance system during MSC culture. Mutations of P53 and epigenetic alterations have been reported to disrupt the quality and quantity of MSC and initiate tumorigenesis...
September 15, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28624056/evaluating-the-efficacy-of-different-types-of-stem-cells-in-preserving-gut-barrier-function-in-necrotizing-enterocolitis
#16
COMPARATIVE STUDY
Christopher J McCulloh, Jacob K Olson, Yijie Wang, Jennifer Vu, Sarah Gartner, Gail E Besner
BACKGROUND: Necrotizing enterocolitis (NEC) is a leading cause of morbidity and mortality in premature infants. Increased intestinal permeability is central to NEC development. We have shown that stem cells (SCs) can reduce the incidence and severity of NEC. Our current goal was to investigate the efficacy of four different types of SC in preservation of gut barrier function during NEC. MATERIALS AND METHODS: We compared (1) amniotic fluid-derived mesenchymal SC, (2) bone marrow-derived mesenchymal SC, (3) amniotic fluid-derived neural SC, and (4) enteric neural SC...
June 15, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28620747/amniotic-fluid-cells-current-progress-and-emerging-challenges-in-renal-regeneration
#17
REVIEW
Stefano Da Sacco, Laura Perin, Sargis Sedrakyan
Amniotic fluid (AF) contains a heterogeneous population of cells that have been identified to possess pluripotent and progenitor-like characteristics. These cells have been applied in various regenerative medicine applications ranging from in vitro cell differentiation to tissue engineering to cellular therapies for different organs including the heart, the liver, the lung, and the kidneys. In this review, we examine the different methodologies used for the derivation of amniotic fluid stem cells and renal progenitors, and their application in renal repair and regeneration...
June 15, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28605059/amniotic-fluid-stem-cells-ameliorate-bladder-dysfunction-induced-by-chronic-bladder-ischemia-in-rat
#18
Ching-Chung Liang, Sheng-Wen Steven Shaw, Yi-Hao Lin, Tsong-Hai Lee
AIMS: This study investigated the protective effect of human amniotic fluid-derived stem cells (hAFSCs) against bladder overactivity in rat model of atherosclerosis-induced chronic bladder ischemia. METHODS: Adult female Sprague-Dawley rats were divided into six groups: (1) Normal control with a regular diet for 8 weeks, (2) Sham-operation, (3) arterial balloon endothelial injury (AEI) of common iliac artery (AEI only), and post-AEI consecutive hAFSCs treatment for (4) 1 day, (5) 3 days, and (6) 7 days...
June 12, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/28591652/fetal-therapy-model-of-myelomeningocele-with-three-dimensional-skin-using-amniotic-fluid-cell-derived-induced-pluripotent-stem-cells
#19
Kazuhiro Kajiwara, Tomohiro Tanemoto, Seiji Wada, Jurii Karibe, Norimasa Ihara, Yu Ikemoto, Tomoyuki Kawasaki, Yoshie Oishi, Osamu Samura, Kohji Okamura, Shuji Takada, Hidenori Akutsu, Haruhiko Sago, Aikou Okamoto, Akihiro Umezawa
Myelomeningocele (MMC) is a congenital disease without genetic abnormalities. Neurological symptoms are irreversibly impaired after birth, and no effective treatment has been reported to date. Only surgical repairs have been reported so far. In this study, we performed antenatal treatment of MMC with an artificial skin using induced pluripotent stem cells (iPSCs) generated from a patient with Down syndrome (AF-T21-iPSCs) and twin-twin transfusion syndrome (AF-TTTS-iPSCs) to a rat model. We manufactured three-dimensional skin with epidermis generated from keratinocytes derived from AF-T21-iPSCs and AF-TTTS-iPSCs and dermis of human fibroblasts and collagen type I...
June 6, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28571656/development-of-a-novel-method-for-amniotic-fluid-stem-cell-storage
#20
Manuela Zavatti, Francesca Beretti, Francesca Casciaro, Giuseppina Comitini, Fabrizia Franchi, Veronica Barbieri, Laura Bertoni, Anto De Pol, Giovanni B La Sala, Tullia Maraldi
BACKGROUND AIMS: Current procedures for collection of human amniotic fluid stem cells (hAFSCs) indicate that cells cultured in a flask for 2 weeks can then be used for research. However, hAFSCs can be retrieved directly from a small amount of amniotic fluid that can be obtained at the time of diagnostic amniocentesis. The aim of this study was to determine whether direct freezing of amniotic fluid cells is able to maintain or improve the potential of a sub-population of stem cells. METHODS: We compared the potential of the hAFSCs regarding timing of freezing, cells obtained directly from amniotic fluid aspiration (D samples) and cells cultured in a flask before freezing (C samples)...
May 29, 2017: Cytotherapy
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