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Amniotic fluid stem cells

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https://www.readbyqxmd.com/read/27873362/influence-of-mesenchymal-stem-cells-conditioned-media-on-proliferation-of-urinary-tract-cancer-cell-lines-and-their-sensitivity-to-ciprofloxacin
#1
Malgorzata Maj, Anna Bajek, Ewelina Nalejska, Dorota Porowinska, Tomasz Kloskowski, Lidia Gackowska, Tomasz Drewa
Mesenchymal stem cells (MSCs) are known to interact with cancer cells through direct cell-to-cell contact and secretion of paracrine factors, although their exact influence on tumor progression in vivo remains unclear. To better understand how fetal and adult stem cells affect tumors, we analyzed viability of human renal (786-0) and bladder (T24) carcinoma cell lines cultured in conditioned media harvested from amniotic fluid-derived stem cells (AFSCs) and adipose-derived stem cells (ASCs). Both media reduced metabolic activity of 786-0 cells, however, decreased viability of T24 cells was noted only after incubation with conditioned medium from ASCs...
November 22, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27845396/first-steps-to-define-murine-amniotic-fluid-stem-cell-microenvironment
#2
E Bertin, M Piccoli, C Franzin, G Spiro, S Donà, A Dedja, F Schiavi, E Taschin, P Bonaldo, P Braghetta, P De Coppi, M Pozzobon
Stem cell niche refers to the microenvironment where stem cells reside in living organisms. Several elements define the niche and regulate stem cell characteristics, such as stromal support cells, gap junctions, soluble factors, extracellular matrix proteins, blood vessels and neural inputs. In the last years, different studies demonstrated the presence of cKit(+) cells in human and murine amniotic fluid, which have been defined as amniotic fluid stem (AFS) cells. Firstly, we characterized the murine cKit(+) cells present both in the amniotic fluid and in the amnion...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27836365/transamniotic-stem-cell-therapy-trascet-in-a-leporine-model-of-gastroschisis
#3
Christina Feng, Christopher D Graham, Hester F Shieh, Joseph A Brazzo, John Patrick Connors, Lucas Rohrer, Alexander Papadakis, David Zurakowski, Dario O Fauza
BACKGROUND/PURPOSE: Transamniotic stem cell therapy (TRASCET) with amniotic fluid mesenchymal stem cells (afMSCs) has been shown to mitigate bowel damage in a rodent model of gastroschisis. As a prerequisite to clinical translation, we sought to study TRASCET in a larger animal model. METHODS: New Zealand rabbit fetuses (n=64) with surgically created gastroschisis were divided into three groups. One group (untreated) had no further manipulations. Two groups received volume-matched intraamniotic injections of either saline or a suspension of afMSCs...
October 26, 2016: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/27818691/osteogenic-differentiation-of-human-amniotic-fluid-mesenchymal-stem-cells-is-determined-by-epigenetic-changes
#4
Monika Glemžaitė, Rūta Navakauskienė
Osteogenic differentiation of human amniotic fluid derived mesenchymal stem cells (AF-MSCs) has been widely studied in vitro and in vivo as a potential tool for regenerative medicine and tissue engineering. While most of the studies analyze changes in transcriptional profile during differentiation to date there is not much information regarding epigenetic changes in AF-MSCs during differentiation. The aim of our study was to evaluate epigenetic changes during osteogenic differentiation of AF-MS cells. Isolated AF-MSCs were characterized morphologically and osteogenic differentiation was confirmed by cell staining and determining expression of alkaline phosphatase and osteopontin by RT-qPCR...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27803714/senescence-associated-molecular-and-epigenetic-alterations-in-mesenchymal-stem-cell-cultures-from-amniotic-fluid-of-normal-and-fetus-affected-pregnancy
#5
Jūratė Savickienė, Sandra Baronaitė, Aistė Zentelytė, Gražina Treigytė, Rūta Navakauskienė
Human amniotic-fluid-derived mesenchymal stem cells (AF-MSCs) are interesting for their multilineage differentiation potential and wide range of therapeutic applications due to the ease of culture expansion. However, MSCs undergo replicative senescence. So far, the molecular mechanisms that underlie fetal diseases and cell senescence are still poorly understood. Here, we analyzed senescence-associated morphologic, molecular, and epigenetic characteristics during propagation of MSCs derived from AF of normal and fetus-affected pregnancy...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27795433/efficient-transformation-of-primary-human-mesenchymal-stromal-cells-by-adenovirus-early-region-1-oncogenes
#6
Thomas Speiseder, Helga Hofmann-Sieber, Estefanía Rodríguez, Anna Schellenberg, Nuray Akyüz, Judith Dierlamm, Thilo Spruss, Claudia Lange, Thomas Dobner
: Previous observations that human amniotic fluid cells (AFC) can be transformed by human adenovirus type 5 (HAdV-5) E1A/E1B oncogenes prompted us to identify the target cells in the AFC population that are susceptible for transformation. Our results demonstrate that one cell type corresponding to mesenchymal stem/stroma cells (hMSC), can be reproducibly transformed by HAdV-5 E1A/E1B oncogenes as efficient as primary rodent cultures. HAdV-5 E1-transformed hMSCs exhibit all properties commonly associated with a high grade of oncogenic transformation, including enhanced cell proliferation, anchorage-independent growth, increased growth rate, high telomerase activity as well as numerical and structural chromosomal aberrations...
October 19, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27688156/mesenchymal-stem-cells-in-the-treatment-of-chronic-lung-disease
#7
Sophie Wecht, Mauricio Rojas
Mesenchymal stem cells (MSCs) are a populace of non-haematopoietic multipotent stromal cells, which have the ability to differentiate into tissue derived from a single germ layer. MSCs have been isolated from various sites, including adipose tissue, skeletal muscle, synovium, spleen, thymus, lung and amniotic fluid, but are most often isolated from bone marrow. MSCs have several valuable functions that make them a promising therapeutic option in the field of regenerative medicine, including the secretion of anti-inflammatory cytokines and growth factors, the migration of cells to the site of injury when administered and the ability to 'rescue' cells through the transfer of functional mitochondria...
November 2016: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/27684534/an-update-clinical-application-of-amniotic-fluid-derived-stem-cells-afscs-in-cancer-cell-therapy-and-tissue-engineering
#8
Shiva Gholizadeh-Ghaleh Aziz, Ezzatollah Fathi, Mohammad Rahmati-Yamchi, Abolfazl Akbarzadeh, Zahra Fardyazar, Maryam Pashaiasl
Recent studies have elucidated that cell-based therapies are promising for cancer treatments. The human amniotic fluid stem (AFS) cells are advantageous cells for such therapeutic schemes that can be innately changed to express therapeutic proteins. HAFSCs display a natural tropism to cancer cells in vivo. They can be useful in cancer cells targeting. Moreover, they are easily available from surplus diagnostic samples during pregnancy and less ethical and legal concern are associated with the collection and application than other putative cells are subjected...
August 10, 2016: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/27651133/evaluation-of-amniotic-mesenchymal-cell-derivatives-on-cytokine-production-in-equine-alveolar-macrophages-an-in-vitro-approach-to-lung-inflammation
#9
Enrica Zucca, Emanuela Corsini, Valentina Galbiati, Anna Lange-Consiglio, Francesco Ferrucci
BACKGROUND: Data obtained in both animal models and clinical trials suggest that cell-based therapies represent a potential therapeutic strategy for lung repair and remodeling. Recently, new therapeutic approaches based on the use of stem cell derivatives (e.g., conditioned medium (CM) and microvesicles (MVs)) to regenerate tissues and improve their functions were proposed. The aim of this study was to investigate the immunomodulatory effects of equine amniotic mesenchymal cell derivatives on lipopolysaccharide (LPS)-induced cytokine production in equine alveolar macrophages, which may be beneficial in lung inflammatory disorders such as recurrent airway obstruction (RAO) in horses...
September 20, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27629493/estrogen-receptor-signaling-in-the-ferutinin-induced-osteoblastic-differentiation-of-human-amniotic-fluid-stem-cells
#10
M Zavatti, M Guida, T Maraldi, F Beretti, L Bertoni, G B La Sala, A De Pol
AIMS: Ferutinin is a daucane sesquiterpene with a high estrogenic activity. Since ferutinin is able to enhance osteoblastic differentiation of human amniotic fluid stem cells (hAFSCs), the aim of this study was to evaluate the role of the estrogen receptors α (ERα) and G-protein coupled receptor 30 (GPR30) in ferutinin-mediated osteoblastic differentiation. Moreover, it was investigated if MEK/ERK and PI3K/Akt signaling pathways are involved in ferutinin-induced effects. MAIN METHODS: hAFSCs were cultured in a standard medium or in an osteoblastic medium for 14 or 21days and ferutinin was added at 10(-8)M...
September 11, 2016: Life Sciences
https://www.readbyqxmd.com/read/27625040/concise-review-mesenchymal-stem-cell-therapy-for-pediatric-disease-perspectives-on-success-and-potential-improvements
#11
Christopher R Nitkin, Tracey L Bonfield
: : Mesenchymal stem cells (MSCs) represent a potentially revolutionary therapy for a wide variety of pediatric diseases, but the optimal cell-based therapeutics for such diversity have not yet been specified. The published clinical trials for pediatric pulmonary, cardiac, orthopedic, endocrine, neurologic, and hematologic diseases provide evidence that MSCs are indeed efficacious, but the significant heterogeneity in therapeutic approaches between studies raises new questions. The purpose of this review is to stimulate new preclinical and clinical trials to investigate these factors...
September 13, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27585525/comparative-study-of-allogenic-and-xenogeneic-mesenchymal-stem-cells-on-cisplatin-induced-acute-kidney-injury-in-sprague-dawley-rats
#12
Rehab H Ashour, Mohamed-Ahdy Saad, Mohamed-Ahmed Sobh, Fatma Al-Husseiny, Mohamed Abouelkheir, Amal Awad, Doaa Elghannam, Hassan Abdel-Ghaffar, Mohamed Sobh
BACKGROUND: The paracrine and regenerative activities of mesenchymal stem cells (MSCs) may vary with different stem cell sources. The aim of the present study is to compare the effects of MSCs from different sources on acute kidney injury (AKI) induced by cisplatin and their influence on renal regeneration. METHODS: A single intraperitoneal injection of cisplatin (5 mg/kg) was used to induce AKI in 120 Sprague-Dawley rats. Rats were treated with either rat bone marrow stem cells (rBMSCs), human adipose tissue-derived stem cells (hADSCs), or human amniotic fluid-derived stem cells (hAFSCs)...
September 1, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27548820/vascular-endothelial-growth-factor-up-regulation-in-human-amniotic-fluid-stem-cell-enhances-nephroprotection-after-ischemia-reperfusion-injury-in-the-rat
#13
Marina Gabriela Monteiro Carvalho Mori da Cunha, Silvia Zia, Diego Vilibaldo Beckmann, Marianne Sylvia Carlon, Fanny Oliveira Arcolino, Maarten Albersen, Ney Luis Pippi, Dominguita Lühers Graça, Conny Gysemans, Peter Carmeliet, Elena Levtchenko, Jan Deprest, Jaan Toelen
OBJECTIVE: To evaluate if the up-regulation of vascular endothelial growth factor strengthens the protective effect of amniotic fluid stem cells in a renal ischemia-reperfusion injury model. DESIGN: Randomized animal study. SETTINGS: University research laboratory. SUBJECTS: A total of 40 males 12-week-old Wistar rats were subjected to ischemia-reperfusion and assigned to four groups: amniotic fluid stem cells, vascular endothelial growth factor-amniotic fluid stem cells in two different doses, and vehicle...
August 19, 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27503204/therapeutic-potential-of-secreted-molecules-derived-from-human-amniotic-fluid-mesenchymal-stem-stroma-cells-in-a-mice-model-of-colitis
#14
E Legaki, M G Roubelakis, G E Theodoropoulos, A Lazaris, A Kollia, G Karamanolis, E Marinos, M Gazouli
Inflammatory bowel diseases (IBDs) are the result of pathological immune responses due to environmental factors or microbial antigens into a genetically predisposed individual. Mainly due to their trophic properties, a mounting interest is focused on the use of human mesenchymal stem/stromal cells (hMSCs) to treat IBD disease in animal models. The aim of the study is to test whether the secreted molecules, derived from a specific population of second trimester amniotic fluid mesenchymal stem/stromal cells, the spindle-shaped MSCs (SS-AF-MSCs), could be utilized as a novel therapeutic, cell free approach for IBD therapy...
October 2016: Stem Cell Reviews
https://www.readbyqxmd.com/read/27465073/human-cardiomyocytes-prior-to-birth-by-integration-free-reprogramming-of-amniotic-fluid-cells
#15
Guihua Jiang, Todd J Herron, Julie Di Bernardo, Kendal A Walker, K Sue O'Shea, Shaun M Kunisaki
: : The establishment of an abundant source of autologous cardiac progenitor cells would represent a major advance toward eventual clinical translation of regenerative medicine strategies in children with prenatally diagnosed congenital heart disease. In support of this concept, we sought to examine whether functional, transgene-free human cardiomyocytes (CMs) with potential for patient-specific and autologous applications could be reliably generated following routine amniocentesis. Under institutional review board approval, amniotic fluid specimens (8-10 ml) at 20 weeks gestation were expanded and reprogrammed toward pluripotency using nonintegrating Sendai virus (SeV) expressing OCT4, SOX2, cMYC, and KLF4...
July 27, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27451014/effects-of-pharmacological-agents-on-human-amniotic-fluid-derived-stem-cells-in-culture
#16
Margaret Dziadosz, Michael Chan, Ross Basch, Bruce K Young
Amniotic fluid contains multipotent cells and could be a source of stem cells for clinical use. Amniotic fluid cells (AFC) are made up of a heterogeneous population of fetal cells that can be retrieved during pregnancy without ethical concernsas it is a standard clinical procedure-. Studies of proliferation, multipotent marker expression, differentiation, and gene expression were performed after culturing with dexamethasone, valproic acid and magnesium sulfate. There were correlations between known drug effects on the human fetus and changes seen in human AFC in culture, as well as previously undescribed observations in neural and chondrogenic inducibility...
July 22, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27444332/the-human-amniotic-fluid-stem-cell-secretome-effectively-counteracts-doxorubicin-induced-cardiotoxicity
#17
Edoardo Lazzarini, Carolina Balbi, Paola Altieri, Ulrich Pfeffer, Elisa Gambini, Marco Canepa, Luigi Varesio, Maria Carla Bosco, Domenico Coviello, Giulio Pompilio, Claudio Brunelli, Ranieri Cancedda, Pietro Ameri, Sveva Bollini
The anthracycline doxorubicin (Dox) is widely used in oncology, but it may cause a cardiomyopathy with bleak prognosis that cannot be effectively prevented. The secretome of human amniotic fluid-derived stem cells (hAFS) has previously been demonstrated to significantly reduce ischemic cardiac damage. Here it is shown that, following hypoxic preconditioning, hAFS conditioned medium (hAFS-CM) antagonizes senescence and apoptosis of cardiomyocytes and cardiac progenitor cells, two major features of Dox cardiotoxicity...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27441756/feasibility-of-human-amniotic-fluid-derived-stem-cells-in-alleviation-of-neuropathic-pain-in-chronic-constrictive-injury-nerve-model
#18
Chien-Yi Chiang, Shih-An Liu, Meei-Ling Sheu, Fu-Chou Chen, Chun-Jung Chen, Hong-Lin Su, Hung-Chuan Pan
PURPOSE: The neurobehavior of neuropathic pain by chronic constriction injury (CCI) of sciatic nerve is very similar to that in humans, and it is accompanied by a profound local inflammation response. In this study, we assess the potentiality of human amniotic fluid derived mesenchymal stem cells (hAFMSCs) for alleviating the neuropathic pain in a chronic constriction nerve injury model. METHODS AND METHODS: This neuropathic pain animal model was conducted by four 3-0 chromic gut ligatures loosely ligated around the left sciatic nerve in Sprague-Dawley rats...
2016: PloS One
https://www.readbyqxmd.com/read/27434028/isolation-characterization-cryopreservation-of-human-amniotic-stem-cells-and-differentiation-to-osteogenic-and-adipogenic-cells
#19
Shiva Gholizadeh-Ghaleh Aziz, Fatima Pashaei-Asl, Zahra Fardyazar, Maryam Pashaiasl
Human stem cells and progenitor cells can be used to treat cancer and replace dysfunctional cells within a tissue or organ. The objective of this study was to identify the appropriate cells type in regenerative medicine and targeted therapy. As an alternative to embryonic and bone marrow stem cells, we examined human amniotic fluid stem cells (hAFSCs), one of the potential source of multipotent stem cells isolated from both cell pellet (using single-stage method), and supernatant of human amniotic fluid. Source of isolation and unique property of the cells emphasize that these cells are one of the promising new tools in therapeutic field...
2016: PloS One
https://www.readbyqxmd.com/read/27426088/amniotic-fluid-derived-mesenchymal-stem-cells-cut-short-the-acuteness-of-cisplatin-induced-nephrotoxicity-in-sprague-dawley-rats
#20
Fatma Al-Husseiny, Mohamed Ahmed Sobh, Rehab H Ashour, Samah Foud, Tarek Medhat, Abdel-Hady El-Gilany, Doaa Elghannam, Hassan Abdel-Ghaffar, Mohamed-Ahdy Saad, Mohamed Sobh
BACKGROUND AND OBJECTIVES: Cisplatin is a nephrotoxic chemotherapeutic agent. So, preventive measures worth to be evaluated. Human amniotic fluid stem cells (hAFSCs) in prevention or amelioration of cisplatin-induced acute kidney injury (AKI) in Sprague-Dawley rates have been tested. METHODS: 80 Sprague-Dawley rats (250~300 g) were used and divided into 4 major groups, 20 rats each. Group I: Saline-injected group. Group II: Cisplatin-injected group (5 mg/kg I.P)...
May 30, 2016: International Journal of Stem Cells
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