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Amniotic fluid stem cells

Sophie Wecht, Mauricio Rojas
Mesenchymal stem cells (MSCs) are a populace of non-haematopoietic multipotent stromal cells, which have the ability to differentiate into tissue derived from a single germ layer. MSCs have been isolated from various sites, including adipose tissue, skeletal muscle, synovium, spleen, thymus, lung and amniotic fluid, but are most often isolated from bone marrow. MSCs have several valuable functions that make them a promising therapeutic option in the field of regenerative medicine, including the secretion of anti-inflammatory cytokines and growth factors, the migration of cells to the site of injury when administered and the ability to 'rescue' cells through the transfer of functional mitochondria...
November 2016: Respirology: Official Journal of the Asian Pacific Society of Respirology
Shiva Gholizadeh-Ghaleh Aziz, Ezzatollah Fathi, Mohammad Rahmati-Yamchi, Abolfazl Akbarzadeh, Zahra Fardyazar, Maryam Pashaiasl
Recent studies have elucidated that cell-based therapies are promising for cancer treatments. The human amniotic fluid stem (AFS) cells are advantageous cells for such therapeutic schemes that can be innately changed to express therapeutic proteins. HAFSCs display a natural tropism to cancer cells in vivo. They can be useful in cancer cells targeting. Moreover, they are easily available from surplus diagnostic samples during pregnancy and less ethical and legal concern are associated with the collection and application than other putative cells are subjected...
August 10, 2016: Artificial Cells, Nanomedicine, and Biotechnology
Enrica Zucca, Emanuela Corsini, Valentina Galbiati, Anna Lange-Consiglio, Francesco Ferrucci
BACKGROUND: Data obtained in both animal models and clinical trials suggest that cell-based therapies represent a potential therapeutic strategy for lung repair and remodeling. Recently, new therapeutic approaches based on the use of stem cell derivatives (e.g., conditioned medium (CM) and microvesicles (MVs)) to regenerate tissues and improve their functions were proposed. The aim of this study was to investigate the immunomodulatory effects of equine amniotic mesenchymal cell derivatives on lipopolysaccharide (LPS)-induced cytokine production in equine alveolar macrophages, which may be beneficial in lung inflammatory disorders such as recurrent airway obstruction (RAO) in horses...
2016: Stem Cell Research & Therapy
M Zavatti, M Guida, T Maraldi, F Beretti, L Bertoni, G B La Sala, A De Pol
AIMS: Ferutinin is a daucane sesquiterpene with a high estrogenic activity. Since ferutinin is able to enhance osteoblastic differentiation of human amniotic fluid stem cells (hAFSCs), the aim of this study was to evaluate the role of the estrogen receptors α (ERα) and G-protein coupled receptor 30 (GPR30) in ferutinin-mediated osteoblastic differentiation. Moreover, it was investigated if MEK/ERK and PI3K/Akt signaling pathways are involved in ferutinin-induced effects. MAIN METHODS: hAFSCs were cultured in a standard medium or in an osteoblastic medium for 14 or 21days and ferutinin was added at 10(-8)M...
September 11, 2016: Life Sciences
Christopher R Nitkin, Tracey L Bonfield
: : Mesenchymal stem cells (MSCs) represent a potentially revolutionary therapy for a wide variety of pediatric diseases, but the optimal cell-based therapeutics for such diversity have not yet been specified. The published clinical trials for pediatric pulmonary, cardiac, orthopedic, endocrine, neurologic, and hematologic diseases provide evidence that MSCs are indeed efficacious, but the significant heterogeneity in therapeutic approaches between studies raises new questions. The purpose of this review is to stimulate new preclinical and clinical trials to investigate these factors...
September 13, 2016: Stem Cells Translational Medicine
Rehab H Ashour, Mohamed-Ahdy Saad, Mohamed-Ahmed Sobh, Fatma Al-Husseiny, Mohamed Abouelkheir, Amal Awad, Doaa Elghannam, Hassan Abdel-Ghaffar, Mohamed Sobh
BACKGROUND: The paracrine and regenerative activities of mesenchymal stem cells (MSCs) may vary with different stem cell sources. The aim of the present study is to compare the effects of MSCs from different sources on acute kidney injury (AKI) induced by cisplatin and their influence on renal regeneration. METHODS: A single intraperitoneal injection of cisplatin (5 mg/kg) was used to induce AKI in 120 Sprague-Dawley rats. Rats were treated with either rat bone marrow stem cells (rBMSCs), human adipose tissue-derived stem cells (hADSCs), or human amniotic fluid-derived stem cells (hAFSCs)...
2016: Stem Cell Research & Therapy
Marina Gabriela Monteiro Carvalho Mori da Cunha, Silvia Zia, Diego Vilibaldo Beckmann, Marianne Sylvia Carlon, Fanny Oliveira Arcolino, Maarten Albersen, Ney Luis Pippi, Dominguita Lühers Graça, Conny Gysemans, Peter Carmeliet, Elena Levtchenko, Jan Deprest, Jaan Toelen
OBJECTIVE: To evaluate if the up-regulation of vascular endothelial growth factor strengthens the protective effect of amniotic fluid stem cells in a renal ischemia-reperfusion injury model. DESIGN: Randomized animal study. SETTINGS: University research laboratory. SUBJECTS: A total of 40 males 12-week-old Wistar rats were subjected to ischemia-reperfusion and assigned to four groups: amniotic fluid stem cells, vascular endothelial growth factor-amniotic fluid stem cells in two different doses, and vehicle...
August 19, 2016: Critical Care Medicine
E Legaki, M G Roubelakis, G E Theodoropoulos, A Lazaris, A Kollia, G Karamanolis, E Marinos, M Gazouli
Inflammatory bowel diseases (IBDs) are the result of pathological immune responses due to environmental factors or microbial antigens into a genetically predisposed individual. Mainly due to their trophic properties, a mounting interest is focused on the use of human mesenchymal stem/stromal cells (hMSCs) to treat IBD disease in animal models. The aim of the study is to test whether the secreted molecules, derived from a specific population of second trimester amniotic fluid mesenchymal stem/stromal cells, the spindle-shaped MSCs (SS-AF-MSCs), could be utilized as a novel therapeutic, cell free approach for IBD therapy...
October 2016: Stem Cell Reviews
Guihua Jiang, Todd J Herron, Julie Di Bernardo, Kendal A Walker, K Sue O'Shea, Shaun M Kunisaki
: : The establishment of an abundant source of autologous cardiac progenitor cells would represent a major advance toward eventual clinical translation of regenerative medicine strategies in children with prenatally diagnosed congenital heart disease. In support of this concept, we sought to examine whether functional, transgene-free human cardiomyocytes (CMs) with potential for patient-specific and autologous applications could be reliably generated following routine amniocentesis. Under institutional review board approval, amniotic fluid specimens (8-10 ml) at 20 weeks gestation were expanded and reprogrammed toward pluripotency using nonintegrating Sendai virus (SeV) expressing OCT4, SOX2, cMYC, and KLF4...
July 27, 2016: Stem Cells Translational Medicine
Margaret Dziadosz, Michael Chan, Ross Basch, Bruce K Young
Amniotic fluid contains multipotent cells and could be a source of stem cells for clinical use. Amniotic fluid cells (AFC) are made up of a heterogeneous population of fetal cells that can be retrieved during pregnancy without ethical concernsas it is a standard clinical procedure-. Studies of proliferation, multipotent marker expression, differentiation, and gene expression were performed after culturing with dexamethasone, valproic acid and magnesium sulfate. There were correlations between known drug effects on the human fetus and changes seen in human AFC in culture, as well as previously undescribed observations in neural and chondrogenic inducibility...
July 22, 2016: Stem Cells and Development
Edoardo Lazzarini, Carolina Balbi, Paola Altieri, Ulrich Pfeffer, Elisa Gambini, Marco Canepa, Luigi Varesio, Maria Carla Bosco, Domenico Coviello, Giulio Pompilio, Claudio Brunelli, Ranieri Cancedda, Pietro Ameri, Sveva Bollini
The anthracycline doxorubicin (Dox) is widely used in oncology, but it may cause a cardiomyopathy with bleak prognosis that cannot be effectively prevented. The secretome of human amniotic fluid-derived stem cells (hAFS) has previously been demonstrated to significantly reduce ischemic cardiac damage. Here it is shown that, following hypoxic preconditioning, hAFS conditioned medium (hAFS-CM) antagonizes senescence and apoptosis of cardiomyocytes and cardiac progenitor cells, two major features of Dox cardiotoxicity...
2016: Scientific Reports
Chien-Yi Chiang, Shih-An Liu, Meei-Ling Sheu, Fu-Chou Chen, Chun-Jung Chen, Hong-Lin Su, Hung-Chuan Pan
PURPOSE: The neurobehavior of neuropathic pain by chronic constriction injury (CCI) of sciatic nerve is very similar to that in humans, and it is accompanied by a profound local inflammation response. In this study, we assess the potentiality of human amniotic fluid derived mesenchymal stem cells (hAFMSCs) for alleviating the neuropathic pain in a chronic constriction nerve injury model. METHODS AND METHODS: This neuropathic pain animal model was conducted by four 3-0 chromic gut ligatures loosely ligated around the left sciatic nerve in Sprague-Dawley rats...
2016: PloS One
Shiva Gholizadeh-Ghaleh Aziz, Fatima Pashaei-Asl, Zahra Fardyazar, Maryam Pashaiasl
Human stem cells and progenitor cells can be used to treat cancer and replace dysfunctional cells within a tissue or organ. The objective of this study was to identify the appropriate cells type in regenerative medicine and targeted therapy. As an alternative to embryonic and bone marrow stem cells, we examined human amniotic fluid stem cells (hAFSCs), one of the potential source of multipotent stem cells isolated from both cell pellet (using single-stage method), and supernatant of human amniotic fluid. Source of isolation and unique property of the cells emphasize that these cells are one of the promising new tools in therapeutic field...
2016: PloS One
Fatma Al-Husseiny, Mohamed Ahmed Sobh, Rehab H Ashour, Samah Foud, Tarek Medhat, Abdel-Hady El-Gilany, Doaa Elghannam, Hassan Abdel-Ghaffar, Mohamed-Ahdy Saad, Mohamed Sobh
BACKGROUND AND OBJECTIVES: Cisplatin is a nephrotoxic chemotherapeutic agent. So, preventive measures worth to be evaluated. Human amniotic fluid stem cells (hAFSCs) in prevention or amelioration of cisplatin-induced acute kidney injury (AKI) in Sprague-Dawley rates have been tested. METHODS: 80 Sprague-Dawley rats (250~300 g) were used and divided into 4 major groups, 20 rats each. Group I: Saline-injected group. Group II: Cisplatin-injected group (5 mg/kg I.P)...
May 30, 2016: International Journal of Stem Cells
A Pantalone, I Antonucci, M Guelfi, P Pantalone, F G Usuelli, L Stuppia, V Salini
OBJECTIVE: Skeletal diseases, both degenerative and secondary to trauma, infections or tumors, represent an ideal target for regenerative medicine and in the last years, stem cells have been considered as good candidates for in vitro and in vivo bone regeneration. To date, several stem cell sources, such as adult mesenchymal stem cells, embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have shown significant osteogenic potential. MATERIALS AND METHODS: In this narrative review, we analyze the possible advantages of the use of AFSCs in the treatment of skeletal diseases, especially through the application of tissue engineering and biomaterials...
July 2016: European Review for Medical and Pharmacological Sciences
Aleksander Skardal, Sean V Murphy, Kathryn Crowell, David Mack, Anthony Atala, Shay Soker
For many cellular therapies being evaluated in preclinical and clinical trials, the mechanisms behind their therapeutic effects appear to be the secretion of growth factors and cytokines, also known as paracrine activity. Often, delivered cells are transient, and half-lives of the growth factors that they secrete are short, limiting their long-term effectiveness. The goal of this study was to optimize a hydrogel system capable of in situ cell delivery that could sequester and release growth factors secreted from those cells after the cells were no longer present...
June 28, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Liyang Gao, Mingyan Zhao, Wei Ye, Jinzhi Huang, Jiaqi Chu, Shouquan Yan, Chaojun Wang, Rong Zeng
The amniotic fluid has a heterogeneous population of cells. Some human amniotic fluid-derived stem (hAFS) cells have been shown to harbor the potential to differentiate into neural cells. However, the neural differentiation efficiency of hAFS cells remains low. In this study, we isolated CD117-positive hAFS cells from amniotic fluid and then examined the pluripotency of these cells through the formation of embryoid bodies (EBs). Additionally, we induced the neural differentiation of these cells using neuroectodermal medium...
August 2016: Tissue & Cell
Juwon Jung, Jin Ah Baek, Hye Won Seol, Young Min Choi
Human embryonic stem cells (hESCs) have been routinely cultured on mouse embryonic fibroblast feederlayers with a medium containing animal materials. For clinical application of hESCs, animal-derived products from the animal feeder cells, animal substrates such as gelatin or Matrigel and animal serum are strictly to be eliminated in the culture system. In this study, we performed that SNUhES32 and H1 were cultured on human amniotic fluid cells (hAFCs) with KOSR XenoFree and a humanized substrate. All of hESCs were relatively well propagated on hAFCs feeders with xeno-free conditions and they expressed pluripotent stem cell markers, alkaline phosphatase, SSEA-4, TRA1-60, TRA1-81, Oct-4, and Nanog like hESCs cultured on STO or human foreskin fibroblast feeders...
March 2016: Balsaeng'gwa Saengsig
M Ghafarzadeh, A Eatemadi, Z Fakhravar
Human amniotic fluid stem cells (hAFSCs) have the ability to self-renew, and multipotent differentiation into three germ layer cells. We obtained 5 ml amniotic fluid from ten 16-20 week pregnant women undergoing amniocentesis. hAFSCs were isolated from all samples, co-cultured with T47D breast cancer cell line and characterized using flow cytometry and RT-PCR. After 3, 4 and 5 days, T47D and HSFCs viability were evaluated with MTT assay. After 5 days of co-culture T47D cells viability were decreased. Our findings showed that hAFSCs can release soluble factors in cell culture, causing an efficient anticancer effect...
2016: Cellular and Molecular Biology
Bajek Anna, Olkowska Joanna, Walentowicz-Sadłecka Małgorzata, Walentowicz Paweł, Sadłecki Paweł, Grabiec Marek, Bodnar Magdalena, Marszałek Andrzej, Dębski Robert, Porowińska Dorota, Czarnecka Joanna, Kaźmierski Łukasz, Drewa Tomasz
AIMS: The aim of the study was to extend the potential use of human stem cells isolated from amniotic fluid in medical applications by confirming their high homogeneity and quality. MATERIALS AND METHODS: Amniotic fluid samples were collected during amniocentesis from 165 women during pregnancy. The proliferation rate, clonogenicity, karyotype, aging process, pluripotent cell markers, expression of surface markers and the potential to differentiate into adipose, bone and cartilage cells of hAFSCs were analyzed...
June 4, 2016: Journal of Cellular Biochemistry
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