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Amniotic fluid stem cells

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https://www.readbyqxmd.com/read/29132800/fetal-bone-marrow-homing-of-donor-mesenchymal-stem-cells-after-transamniotic-stem-cell-therapy-trascet
#1
Hester F Shieh, Azra Ahmed, Sarah A Tracy, David Zurakowski, Dario O Fauza
PURPOSE: Donor cell engraftment patterns following transamniotic stem cell therapy (TRASCET) with amniotic fluid mesenchymal stem cells (afMSCs) are incompatible with solely direct amniotic seeding. We sought to determine whether fetal bone marrow is a component of such engraftment and to examine the chronology of afMSC placental trafficking. METHODS: Two groups of Sprague-Dawley rat fetuses received volume-matched intraamniotic injections on gestational day 17 (E17; term E22): either afMSCs labeled with a luciferase reporter gene or luciferase protein alone...
October 12, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29125510/regenerative-medicine-and-spina-bifida-recent-developments-in-induced-fetal-regeneration
#2
Dario O Fauza
Regenerative medicine as it applies to spina bifida is a multi-pronged endeavor involving spinal cord repair, tissue engineering and fetal regeneration, all of which can mutually overlap to variable extents. The efforts involving spinal cord repair, whether they be cell-based or not, are virtually indistinguishable from the enormous body of work related to spinal cord recovery after traumatic injury. Tissue engineering, on the other hand, can involve a variety of structures besides constructs used for covering the spina bifida defect, for example the urinary bladder, bone, muscle and skin...
October 20, 2017: Journal of Pediatric Rehabilitation Medicine
https://www.readbyqxmd.com/read/29114949/protective-effect-of-gdnf-engineered-amniotic-fluid-derived-stem-cells-on-the-renal-ischaemia-reperfusion-injury-in-vitro
#3
Jia Wang, Fengzhen Wang, Zhuojun Wang, Shulin Li, Lu Chen, Caixia Liu, Dong Sun
OBJECTIVES: Amniotic fluid-derived stem cells (AFSCs) possessing multilineage differentiation potential are proposed as a novel and accessible source for cell-based therapy and tissue regeneration. Glial-derived neurotrophic factor (GDNF) has been hypothesized to promote the therapeutic effect of AFSCs on markedly ameliorating renal dysfunction. The aim of this study was to investigate whether AFSCs equipped with GDNF (GDNF-AFSCs) had capabilities of attenuating mouse renal tubular epithelial cells (mRTECs) apoptosis and evaluate its potential mechanisms...
November 7, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29072700/the-spontaneous-differentiation-and-chromosome-loss-in-ipscs-of-human-trisomy-18-syndrome
#4
Ting Li, Hanzhi Zhao, Xu Han, Jiaying Yao, Lingling Zhang, Ying Guo, Zhen Shao, Ying Jin, Dongmei Lai
Aneuploidy including trisomy results in developmental disabilities and is the leading cause of miscarriages in humans. Unlike trisomy 21, pathogenic mechanisms of trisomy 18 remain unclear. Here, we successfully generated induced pluripotent stem cells (iPSCs) from human amniotic fluid cells (AFCs) with trisomy 18 pregnancies. We found that trisomy 18 iPSCs (18T-iPSCs) were prone to differentiate spontaneously. Intriguingly, 18T-iPSCs lost their extra 18 chromosomes and converted to diploid cells after 10 generations...
October 26, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29070548/excess-iron-considerations-related-to-development-and-early-growth
#5
REVIEW
Marianne Wessling-Resnick
What effects might arise from early life exposures to high iron? This review considers the specific effects of high iron on the brain, stem cells, and the process of erythropoiesis and identifies gaps in our knowledge of what molecular damage may be incurred by oxidative stress that is imparted by high iron status in early life. Specific areas to enhance research on this topic include the following: longitudinal behavioral studies of children to test associations between iron exposures and mood, emotion, cognition, and memory; animal studies to determine epigenetic changes that reprogram brain development and metabolic changes in early life that could be followed through the life course; and the establishment of human epigenetic markers of iron exposures and oxidative stress that could be monitored for early origins of adult chronic diseases...
October 25, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29056972/methotrexate-and-valproic-acid-affect-early-neurogenesis-of-human-amniotic-fluid-stem-cells-from-myelomeningocele
#6
Vardine Sahakyan, Enrico Pozzo, Robin Duelen, Jan Deprest, Maurilio Sampaolesi
Myelomeningocele (MMC) is a severe type of neural tube defect (NTD), in which the backbone and spinal canal do not close completely during early embryonic development. This condition results in serious morbidity and increased mortality after birth. Folic acid significantly reduces, and conversely, folate antagonist methotrexate (MTX) and valproic acid (VPA) increase the occurrence of NTDs, including MMC. How these pharmacological agents exactly influence the early neurulation process is still largely unclear...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28935121/treatment-of-acute-ocular-chemical-burns
#7
REVIEW
Namrata Sharma, Manpreet Kaur, Tushar Agarwal, Virender S Sangwan, Rasik B Vajpayee
Ocular chemical burns are an ophthalmic emergency and are responsible for 11.5% to 22.1% of ocular injuries. Immediate copious irrigation is universally recommended in acute ocular burns to remove the offending agent and minimize damage. Conventional medical therapy consists of the use of agents that promote epithelialization, minimize inflammation, and prevent cicatricial complications. Biological fluids such as autologous serum, umbilical cord blood serum, platelet-rich plasma, and amniotic membrane suspension are a rich source of growth factors and promote healing when used as adjuncts to conventional therapy...
September 18, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/28928772/tissue-engineering-to-repair-diaphragmatic-defect-in-a-rat-model
#8
G P Liao, Y Choi, K Vojnits, H Xue, K Aroom, F Meng, H Y Pan, R A Hetz, C J Corkins, T G Hughes, F Triolo, A Johnson, Kenneth J Moise, K P Lally, C S Cox, Y Li
Tissue engineering is an emerging strategy for repairing damaged tissues or organs. The current study explored using decellularized rat diaphragm scaffolds combined with human amniotic fluid-derived multipotent stromal cells (hAFMSC) to provide a scaffold, stem cell construct that would allow structural barrier function during tissue ingrowth/regeneration. We created an innovative cell infusion system that allowed hAFMSC to embed into scaffolds and then implanted the composite tissues into rats with surgically created left-sided diaphragmatic defects...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28928383/human-stem-cells-alter-the-invasive-properties-of-somatic-cells-via-paracrine-activation-of-mtorc1
#9
Margit Rosner, Ha Thi Thanh Pham, Richard Moriggl, Markus Hengstschläger
Controlled invasion is essential during many physiological processes, whereas its deregulation is a hallmark of cancer. Here we demonstrate that embryonic, induced pluripotent and amniotic fluid stem cells share the property to induce the invasion of primary somatic cells of various origins through insulin-like growth factor I (IGF-I)- or II (IGF-II)-mediated paracrine activation of mechanistic target of rapamycin complex 1 (mTORC1). We propose a model in which downstream of mTORC1 this stem cell-induced invasion is mediated by hypoxia-inducible factor 1-alpha (HIF-1α)-regulated matrix metalloproteinases...
September 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28915235/transamniotic-stem-cell-therapy-a-novel-strategy-for-the-prenatal-management-of-congenital-anomalies
#10
REVIEW
Dario O Fauza
Transamniotic stem cell therapy, or TRASCET, is an emerging therapeutic concept for the management of congenital anomalies based on the augmentation of the biological role of select populations of stem cells that already occur in the amniotic fluid, for targeted therapeutic benefit. Amniotic fluid-derived mesenchymal stem cells (afMSCs) have a central role in the enhanced ability of the fetus to repair tissue damage. This germane recent finding constitutes the biological foundation for the use of afMSCs in TRASCET...
October 11, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28854517/the-effect-of-meconium-exposure-on-the-expression-and-differentiation-of-amniotic-fluid-mesenchymal-stem-cells
#11
T J Jensen, J E Shui, C M Finck
BACKGROUND: The goal of this study was to determine if exposure to meconium would alter the phenotype of amniotic fluid mesenchymal stem cells (AF-MSCs) and the ability of these cells to be differentiated into distal airway type cells. METHODS: Meconium was collected, lyophilized and resuspended in PBS at 3 different concentrations (high, medium, and low). AF-MSCs were cultured in the presence of this meconium suspension for 8 hours and then analyzed for changes in gene expression...
2017: Journal of Neonatal-perinatal Medicine
https://www.readbyqxmd.com/read/28842991/the-amniotic-fluid-as-a-source-of-mesenchymal-stem-cells-with-lung-specific-characteristics
#12
Flore Lesage, Silvia Zia, Julio Jiménez, Jan Deprest, Jaan Toelen
The amniotic fluid is a clinically accessible source of mesenchymal stem cells (AF-MSC) during gestation, which enables autologous cellular therapy for perinatal disorders. The origin of AF-MSC remains elusive: renal and neuronal progenitors have been isolated from the AF-MSC pool, yet no cells with pulmonary characteristics. We analyzed gene expression of pulmonary and renal markers of 212 clonal lines of AF-MSC isolated from amniocentesis samples. AF-MSC were cultured on dishes coated with extracellular matrix (ECM) proteins from decellularized fetal rabbit lungs...
August 26, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28841906/term-amniotic-fluid-an-unexploited-reserve-of-mesenchymal-stromal-cells-for-reprogramming-and-potential-cell-therapy-applications
#13
Roksana Moraghebi, Agnete Kirkeby, Patricia Chaves, Roger E Rönn, Ewa Sitnicka, Malin Parmar, Marcus Larsson, Andreas Herbst, Niels-Bjarne Woods
BACKGROUND: Mesenchymal stromal cells (MSCs) are currently being evaluated in numerous pre-clinical and clinical cell-based therapy studies. Furthermore, there is an increasing interest in exploring alternative uses of these cells in disease modelling, pharmaceutical screening, and regenerative medicine by applying reprogramming technologies. However, the limited availability of MSCs from various sources restricts their use. Term amniotic fluid has been proposed as an alternative source of MSCs...
August 25, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28835139/generation-of-induced-pluripotent-stem-cells-from-amniotic-fluid-cells-of-a-fetus-with-hb-bart-s-disease
#14
Jin-Mei Yan, Dong-Zhi Li
Induced pluripotent stem cells (iPSCs) derived from diseased patients behave as a powerful tool for biomedical research and may provide a source for replacement therapies. In this study, we generated iPSCs from amniotic fluid cells of a fetus with Hb Bart's (γ4) disease (- -/- -). The established iPSCs showed pluripotency similar to that of human embryonic stem cells. They were able to differentiate into various somatic cell types and maintained normal karyotypes after long periods of culture in vitro. The patient-specific iPSCs offer a valuable model for advancing α-thalassemia (α-thal) research and early treatment of the affected fetuses...
August 24, 2017: Hemoglobin
https://www.readbyqxmd.com/read/28752425/minimal-modulation-of-the-host-immune-response-to-sis-matrix-implants-by-mesenchymal-stem-cells-from-the-amniotic-fluid
#15
F Lesage, S Pranpanus, F M Bosisio, M Jacobs, S Ospitalieri, J Toelen, J Deprest
PURPOSE: Surgical restoration of soft tissue defects often requires implantable devices. The clinical outcome of the surgery is determined by the properties inherent to the used matrix. Mesenchymal stem cells (MSC) modulate the immune processes after in vivo transplantation and their addition to matrices is associated with constructive remodeling. Herein we evaluate the potential of MSC derived from the amniotic fluid (AF-MSC), an interesting MSC source for cell therapeutic applications in the perinatal period, for immune modulation when added to a biomaterial...
July 27, 2017: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/28747757/tgf%C3%AE-induced-osteogenic-potential-of-human-amniotic-fluid-stem-cells-via-cd73-generated-adenosine-production
#16
Kwan-Leong Hau, Anna Maria Ranzoni, Filipa Vlahova, Kate Hawkins, Paolo De Coppi, Anna L David, Pascale V Guillot
The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28708036/amniotic-fluid-cells-show-higher-pluripotency-related-gene-expression-than-allantoic-fluid-cells
#17
Debora Kehl, Melanie Generali, Sabrina Görtz, Diego Geering, Jaroslav Slamecka, Simon P Hoerstrup, Ulrich Bleul, Benedikt Weber
Amniotic fluid represents an abundant source of multipotent stem cells, referred as broadly multipotent given their differentiation potential and expression of pluripotency-related genes. However, the origin of this broadly multipotent cellular fraction is not fully understood. Several sources have been proposed so far, including embryonic and extraembryonic tissues. In this regard, the ovine developmental model uniquely allows for direct comparison of fetal fluid-derived cells from two separate fetal fluid cavities, the allantois and the amnion, over the entire duration of gestation...
October 1, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28679310/protein-and-molecular-characterization-of-a-clinically-compliant-amniotic-fluid-stem-cell-derived-extracellular-vesicle-fraction-capable-of-accelerating-muscle-regeneration-through-enhancement-of-angiogenesis
#18
Ben Mellows, Robert Mitchell, Manuela Antonioli, Oliver Kretz, David Chambers, Marie-Theres Zeuner, Bernd Denecke, Luca Musante, Durrgah L Ramachandra, Florence Debacq-Chainiaux, Harry Holthofer, Barbara Joch, Steve Ray, Darius Widera, Anna L David, Tobias B Huber, Joern Dengjel, Paolo De Coppi, Ketan Patel
The secretome of human amniotic fluid stem cells (AFSCs) has great potential as a therapeutic agent in regenerative medicine. However, it must be produced in a clinically compliant manner before it can be used in humans. In this study, we developed a means of producing a biologically active secretome from AFSCs that is free of all exogenous molecules. We demonstrate that the full secretome is capable of promoting stem cell proliferation, migration, and protection of cells against senescence. Furthermore, it has significant anti-inflammatory properties...
September 15, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28675062/lung-regeneration-using-amniotic-fluid-mesenchymal-stem-cells
#19
Alireza Azargoon, Babak Negahdari
Respiratory diseases, such as chronic obstructive pulmonary disease (COPD), pulmonary hypertension and lung fibrosis, are yet a major challenge in the world and they result in irreversible structural lung damage. Lung transplantation as the only therapeutic option face some major challenges like graft rejection and cancer, arising as a result of immunosuppression. A low survival rate faced by lung transplantation patients is presently limited to approximately 5 years. Lungs shortage therefore calls for a mechanism that would increase the availability of suitable organs for transplantation...
July 4, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28653478/different-rna-and-protein-expression-of-surface-markers-in-rabbit-amniotic-fluid-derived-mesenchymal-stem-cells
#20
Michal Kovac, Jaromir Vasicek, Barbora Kulikova, Miroslav Bauer, Jozef Curlej, Andrej Balazi, Peter Chrenek
Over the years, there has been much confusion in defining molecular markers of mesenchymal stem cells (MSCs) for other than human species due to a lack of species-specific antibodies. Therefore, the aim of our study was to define rabbit amniotic fluid-derived mesenchymal stem cells (rAF-MSCs) and to reflect upon the current identification of AF-MSCs by providing a summary of detected surface markers in different species. The expression of rAF-MSC surface markers was analyzed at the protein and mRNA level. Flow cytometry analyses showed that rAF-MSCs were positive for CD29 and CD44, low positive for CD90, but negative for CD73, CD105 and CD166...
June 27, 2017: Biotechnology Progress
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