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https://www.readbyqxmd.com/read/28191448/a-food-grade-expression-system-for-d-psicose-3-epimerase-production-in-bacillus-subtilis-using-an-alanine-racemase-encoding-selection-marker
#1
Jingqi Chen, Zhaoxia Jin, Yuanming Gai, Jibin Sun, Dawei Zhang
BACKGROUND: Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expression system for d-psicose 3-epimerase production, we use an alanine racemase-encoding gene as selection marker in Bacillus subtilis. RESULTS: In this study, the d-alanine racemase-encoding gene dal was deleted from the chromosome of B. subtilis 1A751 using Cre/lox system to generate the food-grade host...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28186321/plasmid-encoded-biosynthetic-genes-alleviate-metabolic-disadvantages-while-increasing-glucose-conversion-to-shikimate-in-an-engineered-escherichia-coli-strain
#2
Alberto Rodriguez, Juan A Martínez, Pierre Millard, Guillermo Gosset, Jean-Charles Portais, Fabien Létisse, Francisco Bolivar
Metabolic engineering strategies applied over the last two decades to produce shikimate (SA) in Escherichia coli have resulted in a battery of strains bearing many expression systems. However, the effects that these systems have on the host physiology and how they impact the production of SA are still not well understood. In this work we utilized an engineered E. coli strain to determine the consequences of carrying a vector that promotes SA production from glucose with a high yield but that is also expected to impose a significant cellular burden...
February 10, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28178978/enhanced-extracellular-production-of-recombinant-proteins-in-escherichia-coli-by-co-expression-with-bacillus-cereus-phospholipase-c
#3
Lingqia Su, Qi Jiang, Lingang Yu, Jing Wu
BACKGROUND: Our laboratory has reported a strategy for improving the extracellular production of recombinant proteins through co-expression with Thermobifida fusca cutinase, which increases membrane permeability via its phospholipid hydrolysis activity. However, the foam generated by the lysophospholipid product makes the fermentation process difficult to control in a fermentor. Phospholipase C (PLC) catalyzes the hydrolysis of phospholipids to produce sn1,2-diacylglycerides and organic phosphate, which do not induce foam formation...
February 8, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28089378/butyrate-production-under-aerobic-growth-conditions-by-engineered-escherichia-coli
#4
Naoya Kataoka, Alisa S Vangnai, Thunyarat Pongtharangkul, Toshiharu Yakushi, Kazunobu Matsushita
Butyrate is an important industrial platform chemical. Although several groups have reported butyrate production under oxygen-limited conditions by a native producer, Clostridium tyrobutylicum, and by a metabolically engineered Escherichia coli, efforts to produce butyrate under aerobic growth conditions have met limited success. Here, we constructed a novel butyrate synthetic pathway that functions under aerobic growth conditions in E. coli, by modifying the 1-butanol synthetic pathway reported previously...
January 11, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28050846/optimization-of-medium-compositions-to-improve-a-novel-glycoprotein-production-by-streptomyces-kanasenisi-zx01
#5
Yong Zhou, Yu-Bo Sun, Hong-Wei He, Jun-Tao Feng, Xing Zhang, Li-Rong Han
Streptomyces kanasenisi ZX01 was found to produce a novel glycoprotein GP-1 previously, which was secreted into medium and had significant activity against tobacco mosaic virus. However, the low production of GP-1 by strain ZX01 limited its further studies. In order to improve the yield of GP-1, a series of statistical experimental design methods were applied to optimize medium of strain ZX01 in this work. Millet medium was chosen to be the optimal original medium for optimization. Soluble starch and yeast extract were identified as the optimal carbon and nitrogen source using one-factor-at-a-time method...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28032228/sweet-sorghum-bagasse-and-corn-stover-serving-as-substrates-for-producing-sophorolipids
#6
Abdul Samad, Ji Zhang, Da Chen, Xiaowen Chen, Melvin Tucker, Yanna Liang
To make the process of producing sophorolipids by Candida bombicola truly sustainable, we investigated production of these biosurfactants on biomass hydrolysates. This study revealed: (1) yield of sophorolipds on bagasse hydrolysate decreased from 0.56 to 0.54 and to 0.37 g/g carbon source when yellow grease was dosed at 10, 40 and 60 g/L, respectively. In the same order, concentration of sophorolipids was 35.9, 41.9, and 39.3 g/L; (2) under similar conditions, sophorolipid yield was 0.12, 0.05 and 0.04 g/g carbon source when corn stover hydrolysate was mixed with soybean oil at 10, 20 and 40 g/L...
December 28, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27959775/ruficoccus-amylovorans-gen-nov-sp-nov-an-amylolytic-and-nitrate-reducing-diazotroph-of-the-family-puniceicoccaceae
#7
Shih-Yao Lin, Asif Hameed, You-Cheng Liu, Yi-Han Hsu, Mei-Hua Hung, Wei-An Lai, Chiu-Chung Young
A novel amylolytic, nitrate-reducing and diazotrophic bacterium, designated strain CC-MHH0563T, isolated from a fermentor was assessed for its taxonomic status using polyphasic taxonomy. Cells of strain CC-MHH0563T was Gram-staining-negative, catalase- and oxidase-positive, mesophilic and aerobic cocci, which produced reddish nondiffusible-pigments. Growth was observed at 15-37 ºС (optimal 25 ºС), pH 6.0-8.0 (optimal pH 7.0) and salinity (0-3 %, w/v). Strain CC-MHH0563T showed highest pair-wise 16S rRNA gene sequence similarity to the members of genera Cerasicoccus (89...
December 12, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27939544/effect-of-feeding-warm-season-annuals-with-orchardgrass-on-ruminal-fermentation-and-methane-output-in-continuous-culture
#8
S L Dillard, A N Hafla, A I Roca-Fernández, A F Brito, M D Rubano, K J Soder
A 4-unit, dual-flow continuous culture fermentor system was used to assess nutrient digestibility, volatile fatty acids (VFA) production, bacterial protein synthesis, and methane (CH4) output of warm-season annual grasses. Treatments were randomly assigned to fermentors in a 4 × 4 Latin square design using 7 d for adaptation to treatment and 3 d for sample collection. Treatments were (1) 100% orchardgrass (Dactylis glomerata L.; ORD); (2) 50% orchardgrass + 50% Japanese millet [Echinochloa esculenta (A. Braun) H...
February 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/27917102/euler-lagrange-computational-fluid-dynamics-for-bio-reactor-scale-down-an-analysis-of-organism-lifelines
#9
Cees Haringa, Wenjun Tang, Amit T Deshmukh, Jianye Xia, Matthias Reuss, Joseph J Heijnen, Robert F Mudde, Henk J Noorman
The trajectories, referred to as lifelines, of individual microorganisms in an industrial scale fermentor under substrate limiting conditions were studied using an Euler-Lagrange computational fluid dynamics approach. The metabolic response to substrate concentration variations along these lifelines provides deep insight in the dynamic environment inside a large-scale fermentor, from the point of view of the microorganisms themselves. We present a novel methodology to evaluate this metabolic response, based on transitions between metabolic "regimes" that can provide a comprehensive statistical insight in the environmental fluctuations experienced by microorganisms inside an industrial bioreactor...
October 2016: Engineering in Life Sciences
https://www.readbyqxmd.com/read/27897254/high-level-expression-of-improved-thermo-stable-alkaline-xylanase-variant-in-pichia-pastoris-through-codon-optimization-multiple-gene-insertion-and-high-density-fermentation
#10
Yihong Lu, Cheng Fang, Qinhong Wang, Yuling Zhou, Guimin Zhang, Yanhe Ma
In paper industry, xylanases are used to increase the pulp properties in bleaching process as its eco-friendly nature. The xylanases activity is hindered by high temperature and alkaline conditions with high enzyme production cost in the paper industry. Here, XynHB, an alkaline stable xylanase from Bacillus pumilus HBP8 was mutated at N188A to XynHBN188A. Expressed mutant in E. coli showed 1.5-fold higher xylanase activity than XynHB at 60 °C. The mutant expressed in Pichia pastoris was glycosylated, remained stable for 30 min at 60 °C...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27861744/omega-3-production-by-fermentation-of-yarrowia-lipolytica-from-fed-batch-to-continuous
#11
Dongming Xie, Edward Miller, Pamela Sharpe, Ethel Jackson, Quinn Zhu
The omega-3 fatty acid, cis-5,8,11,14,17-eicosapentaenoic acid (C20:5; EPA) has wide-ranging benefits in improving heart health, immune function, and mental health. A sustainable source of EPA production through fermentation of metabolically engineered Yarrowia lipolytica has been developed. In this paper, key fed-batch fermentation conditions were identified to achieve 25% EPA in the yeast biomass, which is so far the highest EPA titer reported in the literature. Dynamic models of the EPA fermentation process were established for analyzing, optimizing, and scaling up the fermentation process...
November 14, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27855390/the-role-of-the-ydib-gene-which-encodes-quinate-shikimate-dehydrogenase-in-the-production-of-quinic-dehydroshikimic-and-shikimic-acids-in-a-pts-strain-of-escherichia-coli
#12
Sofía García, Noemí Flores, Ramón De Anda, Georgina Hernández, Guillermo Gosset, Francisco Bolívar, Adelfo Escalante
The culture of engineered Escherichia coli for shikimic acid (SA) production results in the synthesis of quinic acid (QA) and dehydroshikimic acid (DHS), reducing SA yield and impairing downstream processes. The synthesis of QA by quinate/shikimate dehydrogenase (YdiB, ydiB) has been previously proposed; however, the precise role for this enzyme in the production of QA in engineered strains of E. coli for SA production remains unclear. We report the effect of the inactivation or the overexpression of ydiB in E...
2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27830360/efficient-utilization-of-eucheuma-denticulatum-hydrolysates-using-an-activated-carbon-adsorption-process-for-ethanol-production-in-a-5-l-fermentor
#13
Chae Hun Ra, Min Ji Kim, Gwi-Taek Jeong, Sung-Koo Kim
A total monosaccharide concentration of 37.8 g/L and 85.9% conversion from total fermentable monosaccharides of 44.0 g/L from 110 g dw/L Eucheuma denticulatum slurry were obtained by thermal acid hydrolysis and enzymatic saccharification. Subsequent adsorption treatment to remove 5-hydroxymethylfurfural (5-HMF) using 5% activated carbon and an adsorption time of 10 min were used to prevent a prolonged lag phase, reduced cell growth, and low ethanol production. The equilibrium adsorption capacity (q e) of HMF (58...
November 9, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27743774/consolidated-bioprocessing-of-microalgal-biomass-to-carboxylates-by-a-mixed-culture-of-cow-rumen-bacteria-using-anaerobic-sequencing-batch-reactor-asbr
#14
Baisuo Zhao, Jie Liu, Craig Frear, Mark Holtzapple, Shulin Chen
This study employed mixed-culture consolidated bioprocessing (CBP) to digest microalgal biomass in an anaerobic sequencing batch reactor (ASBR). The primary objectives are to evaluate the impact of hydraulic residence time (HRT) on the productivity of carboxylic acids and to characterize the bacterial community. HRT affects the production rate and patterns of carboxylic acids. For the 5-L laboratory-scale fermentation, a 12-day HRT was selected because it offered the highest productivity of carboxylic acids and it synthesized longer chains...
December 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27723092/in-situ-enhancement-of-surfactin-biosynthesis-in-bacillus-subtilis-using-novel-artificial-inducible-promoters
#15
Song Jiao, Xu Li, Huimin Yu, Huan Yang, Xue Li, Zhongyao Shen
Surfactin-family lipopeptides are green biosurfactants with substantial industrial potential. The major problem prohibiting surfactin use is the low titer of the wild producer, Bacillus subtilis. Using transcriptomic analysis, four strong promoters, PgroE, Pcdd, PrplK, and PsspE, were identified and cloned from the genome of B. subtilis THY-7, a novel surfactin producer that has been identified from soil with a 0.55 g/L surfactin titer. An optimal promoter, PgroE, was selected to replace the native THY-7 surfactin synthase (SrfA) promoter through single-cross homologous recombination; however, the resulting engineered strain containing the PgroE substitution did not synthesize surfactin...
October 10, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27708366/biotransformation-of-ferulic-acid-to-vanillin-in-the-packed-bed-stirred-fermentors
#16
Lei Yan, Peng Chen, Shuang Zhang, Suyue Li, Xiaojuan Yan, Ningbo Wang, Ning Liang, Hongyu Li
We performed the biotransformation of ferulic acid to vanillin using Bacillus subtilis (B. subtilis) in the stirring packed-bed reactors filled with carbon fiber textiles (CFT). Scanning electron microscope (SEM), HPLC, qRT-PCR and ATP assay indicated that vanillin biotransformation is tightly related to cell growth, cellar activity and the extent of biofilm formation. The biotransformation was affected by hydraulic retention time (HRT), temperature, initial pH, stirring speed and ferulic acid concentration, and the maximum vanillin production was obtained at 20 h, 35 °C, 9...
October 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27640008/high-level-expression-and-biochemical-characterization-of-a-novel-cold-active-lipase-from-rhizomucor-endophyticus
#17
Xiaojie Duan, Mingming Zheng, Yu Liu, Zhengqiang Jiang, Shaoqing Yang
OBJECTIVES: To identify novel cold-active lipases from fungal sources and improve their production by heterologous expression in Pichia pastoris. RESULTS: A novel cold-active lipase gene (ReLipB) from Rhizomucor endophyticus was cloned. ReLipB was expressed at a high level in Pichia pastoris using high cell-density fermentation in a 5-l fermentor with the highest lipase activity of 1395 U/ml. The recombinant lipase (RelipB) was purified and biochemically characterized...
December 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27623797/application-of-crispri-in-corynebacterium-glutamicum-for-shikimic-acid-production
#18
Bo Zhang, Zhi-Qiang Liu, Chang Liu, Yu-Guo Zheng
OBJECTIVES: To construct, test and exploit the CRISPRi system for enhancement of shikimic acid production with Corynebacterium glutamicum. RESULTS: The CRISPRi system was used to regulate C. glutamicum gene expression at the transcriptional level. Hfq protein-mediated small regulatory RNAs system was compared with CRISPRi system. The more efficient CRISPRi system was used to adjust the metabolic flux involving the shikimic acid (SA) synthetic pathway. In 11 candidate genes, including transcription regulator, three targets were effective for increasing SA production...
December 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27611026/high-efficiency-cell-recycle-continuous-sodium-gluconate-production-by-aspergillus-niger-using-on-line-physiological-parameters-association-analysis-to-regulate-feed-rate-rationally
#19
Fei Lu, Chao Li, Zejian Wang, Wei Zhao, Ju Chu, Yingping Zhuang, Siliang Zhang
In this paper, a system of cell-recycle continuous fermentation for sodium gluconate (SG) production by Aspergillus niger (A. niger) was established. Based on initial continuous fermentation result (100.0h) with constant feed rate, an automatic feedback strategy to regulate feed rate using on-line physiological parameters (OUR and DO) was proposed and applied successfully for the first time in the improved continuous fermentation (240.5h). Due to less auxiliary time, highest SG production rate (31.05±0.29gL(-1)h(-1)) and highest yield (0...
November 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27558633/improving-the-production-of-l-phenylalanine-by-identifying-key-enzymes-through-multi-enzyme-reaction-system-in-vitro
#20
Dongqin Ding, Yongfei Liu, Yiran Xu, Ping Zheng, Haixing Li, Dawei Zhang, Jibin Sun
L-Phenylalanine (L-Phe) is an important amino acid used in both food and medicinal applications. We developed an in vitro system that allowed a direct, quantitative investigation of phenylalanine biosynthesis in E. coli. Here, the absolute concentrations of six enzymes (AroK, AroL, AroA, AroC, PheA and TyrB) involved in the shikimate (SHIK) pathway were determined by a quantitative proteomics approach and in vitro enzyme titration experiments. The reconstitution of an in vitro reaction system for these six enzymes was established and their effects on the phenylalanine production were tested...
2016: Scientific Reports
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