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https://www.readbyqxmd.com/read/29333753/comparative-performance-of-different-scale-down-simulators-of-substrate-gradients-in-penicillium-chrysogenum-cultures-the-need-of-a-biological-systems-response-analysis
#1
Guan Wang, Junfei Zhao, Cees Haringa, Wenjun Tang, Jianye Xia, Ju Chu, Yingping Zhuang, Siliang Zhang, Amit T Deshmukh, Walter van Gulik, Joseph J Heijnen, Henk J Noorman
In a 54 m3 large-scale penicillin fermentor, the cells experience substrate gradient cycles at the timescales of global mixing time about 20-40 s. Here, we used an intermittent feeding regime (IFR) and a two-compartment reactor (TCR) to mimic these substrate gradients at laboratory-scale continuous cultures. The IFR was applied to simulate substrate dynamics experienced by the cells at full scale at timescales of tens of seconds to minutes (30 s, 3 min and 6 min), while the TCR was designed to simulate substrate gradients at an applied mean residence time (τc) of 6 min...
January 15, 2018: Microbial Biotechnology
https://www.readbyqxmd.com/read/29324690/effects-of-agitation-aeration-and-temperature-on-production-of-a-novel-glycoprotein-gp-1-by-streptomyces-kanasenisi-zx01-and-scale-up-based-on-volumetric-oxygen-transfer-coefficient
#2
Yong Zhou, Li-Rong Han, Hong-Wei He, Bu Sang, Dai-Lin Yu, Jun-Tao Feng, Xing Zhang
The effects of temperature, agitation and aeration on glycoprotein GP-1 production by Streptomyces kanasenisi ZX01 in bench-scale fermentors were systematically investigated. The maximum final GP-1 production was achieved at an agitation speed of 200 rpm, aeration rate of 2.0 vvm and temperature of 30 °C. By using a dynamic gassing out method, the effects of agitation and aeration on volumetric oxygen transfer coefficient (kLa) were also studied. The values of volumetric oxygen transfer coefficient in the logarithmic phase increased with increase of agitation speed (from 14...
January 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29320442/optimization-of-fermentation-conditions-and-bench-scale-for-improvement-of-a-novel-glycoprotein-gp-1-production-by-streptomyces-kanasenisi-zx01
#3
Yong Zhou, Xin Zhou, Dai-Lin Yu, Bu Sang, Jun-Tao Feng, Li-Rong Han, Xing Zhang
GP-1 is a novel glycoprotein produced by Streptomyces kanasenisi ZX01 that was isolated from soil near Kanas Lake with significant bioactivity against tobacco mosaic virus. However, extremely low fermentation production has largely hindered further research and market applications on glycoprotein GP-1. In this study, response surface methodology was used to optimize fermentation conditions in a shake flask for higher glycoprotein GP-1 production. When the optimized fermentation conditions were inoculum volume of 6%, initial pH of 6...
January 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29308528/biosynthesis-of-flavone-c-glucosides-in-engineered-escherichia-coli
#4
Anil Shrestha, Ramesh Prasad Pandey, Dipesh Dhakal, Prakash Parajuli, Jae Kyung Sohng
Two plant-originated C-glucosyltransferases (CGTs) UGT708D1 from Glycine max and GtUF6CGT1 from Gentiana triflora were accessed for glucosylation of selected flavones chrysin and luteolin. Uridine diphosphate (UDP)-glucose pool was enhanced in Escherichia coli cell cytosol by introducing heterologous UDP-glucose biosynthetic genes, i.e., glucokinase (glk), phosphoglucomutase (pgm2), and glucose 1-phosphate uridylyltransferase (galU), along with glucose facilitator diffusion protein from (glf) from different organisms, in a multi-monocistronic vector with individual T7 promoter, ribosome binding site, and terminator for each gene...
January 7, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29305513/production-of-4-hydroxybenzoic-acid-by-an-aerobic-growth-arrested-bioprocess-using-metabolically-engineered-corynebacterium-glutamicum
#5
Yukihiro Kitade, Ryoma Hashimoto, Masako Suda, Kazumi Hiraga, Masayuki Inui
Corynebacterium glutamicum was metabolically engineered to produce 4-hydroxybenzoic acid (4-HBA), a valuable aromatic compound used as a raw material for the production of liquid crystal polymers and paraben. C. glutamicum was found to have a higher tolerance to 4-HBA toxicity than previously reported hosts used for the production of genetically engineered 4-HBA. To obtain higher titers of 4-HBA, we employed a stepwise overexpression of all seven target genes in the shikimate pathway in C. glutamicum Specifically, multiple chromosomal integrations of a mutated aroG from Escherichia coli, encoding a DAHP synthase, and wild-type aroCKB from C...
January 5, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29202526/-production-of-l-citrulline-by-a-recombinant-corynebacterium-crenatum-sypa-5-5-whole-cell-biocatalyst
#6
Qianni Liu, Meijuan Xu, Rongzhen Zhang, Meizhou Wang, Xian Zhang, Taowei Yang, Zhiming Rao
Arginine deiminase (ADI) was first high-efficient expressed in Corynebacterium crenatum SYPA 5-5. The ADI was purified by Ni-NTA affinity chromatography and SDS-PAGE analysis showed the molecular weight (MW) was 46.8 kDa. The optimal temperature and pH of ADI were 37 ℃ and 6.5 respectively. The Michaelis constant was 12.18 mmol/L and the maximum velocity was 0.36 μmol/(min·mL). Under optimal conditions, 300 g/L of arginine was transformed and the productivity reach 8 g/(L·h). The recombinant strain was cultivated in a 5-L fermentor and used for whole-cell transformation of 300 g/L arginine, under repeated-batch bioconversion, the cumulative production reached 1 900 g/L...
November 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29194020/an-anaerobic-detoxification-fermentation-by-rhodospirillum-rubrum-for-rice-straw-as-feed-with-moderate-pretreatment
#7
Jian Zhang, Jie Yuan, Wen-Xue Zhang, Fang Tu, Ya Jiang, Chuan-Ze Sun
A novel and effective process was put forward for converting rice straw into feed by combining diluted acid hydrolysis and ammonization with Rhodospirillum rubrum fermentation. After pretreatment with dilute sulfuric or phosphoric acid (1%, w/w) at 100°C, materials were subjected to fermentation under several gases (N2, CO2, and air) and different light intensities in a 2-L fermentor. The key indexes of feed for fermented materials were estimated and several toxic substances were investigated during the fermentation...
December 1, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29177886/production-of-poly-%C3%AE-glutamic-acid-by-a-thermotolerant-glutamate-independent-strain-and-comparative-analysis-of-the-glutamate-dependent-difference
#8
Wei Zeng, Guiguang Chen, Ye Guo, Bin Zhang, Mengna Dong, Yange Wu, Jun Wang, Zhiqun Che, Zhiqun Liang
Poly-γ-glutamic acid (γ-PGA) is a promising microbial polymer with wide applications in industry, agriculture and medicine. In this study, a novel glutamate-independent γ-PGA producing strain with thermotolerant characteristics was isolated and identified as Bacillus subtilis GXG-5, then its product was also characterized. The fermentation process was optimized by single-factor tests, and results showed that high temperature (50 °C) was especially suitable for the γ-PGA production by GXG-5. The γ-PGA yield reached 19...
November 25, 2017: AMB Express
https://www.readbyqxmd.com/read/29150708/production-of-arabitol-from-enzymatic-hydrolysate-of-soybean-flour-by-debaryomyces-hansenii-fermentation
#9
Abdullah A Loman, S M M Islam, Lu-Kwang Ju
Arabitol is a low-calorie sugar alcohol with anti-cariogenic properties. Enzymatic hydrolysate of soybean flour is a new renewable biorefinery feedstock containing hexose, pentose, and organic nitrogen sources. Arabitol production by Debaryomyces hansenii using soybean flour hydrolysate was investigated. Effects of medium composition, operating conditions, and culture stage (growing or stationary phase) were studied. Production was also compared at different culture volumes to understand the effect of dissolved oxygen concentration (DO)...
November 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29074856/engineering-a-synthetic-pathway-for-maleate-in-escherichia-coli
#10
Shuhei Noda, Tomokazu Shirai, Yutaro Mori, Sachiko Oyama, Akihiko Kondo
Maleate is one of the most important dicarboxylic acids and is used to produce various polymer compounds and pharmaceuticals. Herein, microbial production of maleate is successfully achieved, to our knowledge for the first time, using genetically modified Escherichia coli. A synthetic pathway of maleate is constructed in E. coli by combining the polyketide biosynthesis pathway and benzene ring cleavage pathway. The metabolic engineering approach used to fine-tune the synthetic pathway drastically improves maleate production and demonstrates that one of the rate limiting steps exists in the conversion of chorismate to gentisate...
October 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/28992119/a-reproducible-and-scalable-procedure-for-preparing-bacterial-extracts-for-cell-free-protein-synthesis
#11
Kazushige Katsura, Takayoshi Matsuda, Yuri Tomabechi, Mayumi Yonemochi, Kazuharu Hanada, Noboru Ohsawa, Kensaku Sakamoto, Chie Takemoto, Mikako Shirouzu
Cell-free protein synthesis is a useful method for preparing proteins for functional or structural analyses. However, batch-to-batch variability with regard to protein synthesis activity remains a problem for large-scale production of cell extract in the laboratory. To address this issue, we have developed a novel procedure for large-scale preparation of bacterial cell extract with high protein synthesis activity. The developed procedure comprises cell cultivation using a fermentor, harvesting and washing of cells by tangential flow filtration, cell disruption with high-pressure homogenizer and continuous diafiltration...
November 1, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28948457/an-aerobic-detoxification-photofermentation-by-rhodospirillum-rubrum-for-converting-soy-sauce-residue-into-feed-with-moderate-pretreatment
#12
Jian Zhang, Jie Yuan, Wen-Xue Zhang, Wen-You Zhu, Fang Tu, Ya Jiang, Chuan-Ze Sun
This paper reports an effective process for converting soy sauce residue into feeds by combining moderate acid hydrolysis and ammonization with Rhodospirillum rubrum fermentation. After pretreatment with dilute sulfuric or phosphoric acid (1%, w/w) at 100 °C, materials were subjected to fermentation under several gases (N2, CO2, and air) and different light intensities in a 2-L fermentor. Following sulfuric acid treatment, the true protein increased from 188 to 362 g kg(-1) and the crude fiber decreased from 226 to 66 g kg(-1) after fermentation at 0...
September 25, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28932264/high-level-expression-and-characterization-of-a-novel-cutinase-from-malbranchea-cinnamomea-suitable-for-butyl-butyrate-production
#13
Xiaojie Duan, Yu Liu, Xin You, Zhengqiang Jiang, Shaoxiang Yang, Shaoqing Yang
BACKGROUND: Butyl butyrate has been considered as a promising fuel source because it is a kind of natural ester which can be converted from renewable and sustainable lignocellulosic biomass. Compared with the conventional chemical methods for butyl butyrate production, the enzymatic approach has been demonstrated to be more attractive, mainly owing to the mild reaction conditions, high specificity, low energy consumption, and environmental friendliness. Cutinases play an important role in the butyl butyrate production process...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28931206/metabolic-engineering-a-yeast-to-produce-astaxanthin
#14
Yu-Ju Lin, Jui-Jen Chang, Hao-Yeh Lin, Caroline Thia, Yi-Ying Kao, Chieh-Chen Huang, Wen-Hsiung Li
In this study, an astaxanthin-biosynthesis Kluyveromyces marxianus strain Sm23 was first constructed, which could produce 31µg/g DCW astaxanthin. Then, repeated genome integration of the key astaxanthin biosynthesis genes Hpchyb and bkt was done to increase gene copy number and astaxanthin yield. Four improved strains were obtained and the yield of astaxanthin and the total yield of carotenoids in a strain increased with the copy numbers of Hpchyb and bkt. To improve the yield further, the gene Hpchyb from Haematococcus pluvialis was modified by site-directed mutagenesis to increase the enzyme efficiency or/and to prevent the heterologous protein degradation by ubiquitination...
December 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28920400/-production-of-gastric-mucosa-protective-collagen-%C3%A2-by-pichia-pastoris
#15
Weina Li, Zifang Shang, Zhiguang Duan, Linbo Li, Jing He, Daidi Fan
To improve collagen production by recombinant Pichia pastoris, we applied Placket-Burman and Box-Behnken design to optimize the fermentation medium. Through Placket-Burman design, three variables in the medium (concentration of yeast extract, peptone and glycerol) were selected for having significant effect on cell dry weight. Through Box-Behnken design regression coefficients analysis, a secondary degree polynomial equation was established, and the optimum levels of the three variables were yeast extract 1...
April 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28912077/electron-receptor-addition-enhances-butanol-synthesis-in-abe-fermentation-by-clostridium-acetobutylicum
#16
Jian Ding, Hongzhen Luo, Fang Xie, Hao Wang, Meng Xu, Zhongping Shi
The techniques for enhancing butanol production in ABE fermentation by Clostridium acetobutylicum generally focus on adding electron carrier to strengthen NADH synthesis, repressing hydrogenase by aerating CO, supplementing butyrate, etc. However, those methods suffer from the problems of total solvent decrease, high purification cost, using expensive supplemental substances, etc. In this study, we added small amount of electron receptors (Na2SO4/CaSO4, 2g/L) into ABE fermentation broth: to alter electron/proton distributions in the intracellular electron transport shuttle system, directing more electron/proton pairs into NADH synthesis route; to stimulate intracellular accumulation of those amino acids favorable for cells survival/butanol synthesis...
September 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28876030/-overexpression-and-characterization-of-endo-cellulase-a10-from-aspergillus-nidulans-in-pichia-pastoris
#17
Jianjun Li, Xiaolian Li, Ziqiang Wang, Jianjun Li
Endo-cellulases are important to efficiently hydrolyze cellulose, and widely used in biotechnology. In this study, we overexpressed and characterized an endo-cellulase from Aspergillus nidulans. This endo-cellulase was successfully overexpressed in flasks and fermentor, and its concentration in fermentor reached 0.89 mg/mL. The optimal pH and temperature of the were 4.0 and 80 ℃ respectively, and it was very stable between pH 2.0 and 12.0. It was thermally stable below 60 ℃, whereas it was inactivated very quickly above 70 ℃...
May 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28861520/arginine-exposure-decreases-acidogenesis-in-long-term-oral-biofilm-microcosms
#18
Ruth G Ledder, Hitesh Mistry, Prem K Sreenivasan, Gavin Humphreys, Andrew J McBain
Arginine is an important pH-elevating agent in the oral cavity. It has been incorporated in oral hygiene formulations to mitigate sensitivity and to prevent caries. In this investigation, the effects of sustained arginine dosing of dental plaque microcosms on bacteriological composition and pH were evaluated under controlled conditions. Plaque microcosms were established in constant-depth film fermentors (CDFFs) using salivary inocula and fed continuously with artificial saliva. To simulate resting and cariogenic states, the CDFFs were supplemented with sterile water or 5% sucrose, respectively...
July 2017: MSphere
https://www.readbyqxmd.com/read/28831560/a-homofermentative-bacillus-sp-bc-001-and-its-performance-as-a-potential-l-lactate-industrial-strain
#19
Sitanan Thitiprasert, Kentaro Kodama, Somboon Tanasupawat, Phatthanon Prasitchoke, Tanapawarin Rampai, Budsabathip Prasirtsak, Vasana Tolieng, Jiraporn Piluk, Suttichai Assabumrungrat, Nuttha Thongchul
Bacillus sp. BC-001 was first reported as a potent thermotolerant and homofermentative strain for an industrial-scale L-lactate production. In a flask culture, this isolate fermented both glucose and sucrose to lactate with high yield (0.96 and 0.87 g/g) and productivity (2.8 and 2.6 g/L h), respectively. The higher lactate production performance was obtained in the simultaneous saccharification and fermentation of liquefied starch (150.1 g/L final titer, 0.98 g/g yield, 3.2 g/L h productivity) and the fed-batch glucose fermentation (139...
December 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28803103/engineering-halomonas-bluephagenesis-td01-for-non-sterile-production-of-poly-3-hydroxybutyrate-co-4-hydroxybutyrate
#20
Xiangbin Chen, Jin Yin, Jianwen Ye, Haoqian Zhang, Xuemei Che, Yiming Ma, Mengyi Li, Lin-Ping Wu, Guo-Qiang Chen
Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), short as P(3HB-co-4HB), was successfully produced by engineered Halomonas bluephagenesis TD01 grown in glucose and γ-butyrolactone under open non-sterile conditions. Gene orfZ encoding 4HB-CoA transferase of Clostridium kluyveri was integrated into the genome to achieve P(3HB-co-4HB) accumulation comparable to that of strains encoding orfZ on plasmids. Fed-batch cultivations conducted in 1-L and 7-L fermentors, respectively, resulted in over 70g/L cell dry weight (CDW) containing 63% P(3HB-co-12mol% 4HB) after 48h under non-sterile conditions...
November 2017: Bioresource Technology
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