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https://www.readbyqxmd.com/read/28548573/inulin-type-fructan-degradation-capacity-of-clostridium-cluster-iv-and-xiva-butyrate-producing-colon-bacteria-and-their-associated-metabolic-outcomes
#1
F Moens, L De Vuyst
Four selected butyrate-producing colon bacterial strains belonging to Clostridium cluster IV (Butyricicoccus pullicaecorum DSM 23266(T) and Faecalibacterium prausnitzii DSM 17677(T)) and XIVa (Eubacterium hallii DSM 17630 and Eubacterium rectale CIP 105953(T)) were studied as to their capacity to degrade inulin-type fructans and concomitant metabolite production. Cultivation of these strains was performed in bottles and fermentors containing a modified medium for colon bacteria, including acetate, supplemented with either fructose, oligofructose, or inulin as the sole energy source...
May 30, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28484256/improved-thermostability-of-an-acidic-xylanase-from-aspergillus-sulphureus-by-combined-disulphide-bridge-introduction-and-proline-residue-substitution
#2
Wenhan Yang, Yongzhi Yang, Lingdi Zhang, Hang Xu, Xiaojing Guo, Xu Yang, Bing Dong, Yunhe Cao
As a feed additive, xylanase has been widely applied in the feed of monogastric animals, which contains multiple plant polysaccharides. However, during feed manufacture, the high pelleting temperatures challenge wild-type xylanases. The aim of this study was to improve the thermostability of Aspergillus sulphureus acidic xylanase. According to the predicted protein structure, a series of disulphide bridges and proline substitutions were created in the xylanase by PCR, and the mutants were expressed in Pichia pastoris...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28456470/simple-and-rapid-method-for-isolation-and-quantitation-of-polyhydroxyalkanoate-by-sds-sonication-treatment
#3
Hisashi Arikawa, Shunsuke Sato, Tetsuya Fujiki, Keiji Matsumoto
We developed a new method for isolation and quantitation of polyhydroxyalkanoate (PHA) from culture broth. In this method, the cells were sonicated in sodium dodecyl sulfate (SDS) solution and centrifuged to recover PHA. The recovered PHA was rinsed with deionized water and ethanol, and then weighed after drying. Hazardous chemicals such as chloroform, methanol, and sulfuric acid were not used, and no expensive analytical instruments were needed. We applied this method to Cupriavidus necator culture broths that included various amounts of poly(3-hydroxybutyrate) (PHB) or poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) from flasks and jar fermentors...
April 26, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28456412/association-of-aqueous-hydrogen-concentration-with-methane-production-in-continuous-cultures-modulated-to-vary-ph-and-solids-passage-rate
#4
B A Wenner, J de Souza, F Batistel, T J Hackmann, Z Yu, J L Firkins
The objective of this study was to evaluate the effects of altering pH and solids passage rate (kp) on concentration of aqueous H2 [H2(aq)], CH4 production, volatile fatty acids (VFA) production, and fiber digestibility in a continuous culture fermentation system. The present study was conducted as a 2 × 2 factorial treatment arrangement in a Latin square design using continuous culture fermentors (n = 4). Our continuous culture system was converted to a closed system to measure CH4 and H2 emission while measuring H2(aq) concentration and VFA production for complete stoichiometric assessment of fermentation pattern...
April 26, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28456405/effects-of-replacing-soybean-meal-with-canola-meal-differing-in-rumen-undegradable-protein-content-on-ruminal-fermentation-and-gas-production-kinetics-using-2-in-vitro-systems
#5
E M Paula, H F Monteiro, L G Silva, P D B Benedeti, J L P Daniel, T Shenkoru, G A Broderick, A P Faciola
Previous research indicated that there were significant differences in rumen-undegradable protein (RUP) among canola meals (CM), which could influence the nutritional value of CM. The objectives of this study were to (1) evaluate the effects of feeding CM with different RUP contents on ruminal fermentation, nutrient digestion, and microbial growth using a dual-flow continuous culture system (experiment 1) and (2) evaluate ruminal gas production kinetics, in vitro organic matter (OM) digestibility, and methane (CH4) production of soybean meal (SBM) and CM with low or high RUP in the diet or as a sole ingredient using a gas production system (experiments 2 and 3)...
April 26, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28396694/synthesis-of-umbelliferone-derivatives-in-escherichia-coli-and-their-biological-activities
#6
Luan Luong Chu, Ramesh Prasad Pandey, Haet Nim Lim, Hye Jin Jung, Nguyen Huy Thuan, Tae-Su Kim, Jae Kyung Sohng
BACKGROUND: Umbelliferone, also known as 7-hydroxycoumarin, is a phenolic metabolite found in many familiar plants. Its derivatives have been shown to have various pharmacological and chemo-preventive effects on human health. A uridine diphosphate glycosyltransferase YjiC from Bacillus licheniformis DSM 13, a cytochrome P450BM3 (CYP450 BM3) variant namely mutant 13 (M13) from Bacillus megaterium, and an O-methyltransferase from Streptomyces avermitilis (SaOMT2) were used for modifications of umbelliferone...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28372242/green-synthesis-of-isomaltulose-from-cane-molasses-by-bacillus-subtilis-wb800-pha01-pali-in-a-biologic-membrane-reactor
#7
Lingtian Wu, Shanshan Wu, Juanjuan Qiu, Chuanxue Xu, Sha Li, Hong Xu
A green process and environmentally benign process is highly desirable in the development of enzymatic catalysis. In this work, the shuttle plasmid pHA01 was constructed and the sucrose isomerase (SIase) was expressed in Bacillus subtilis WB800. The optimal nitrogen and carbon sources for SIase expression were yeast extract (15g/L) and un-pretreated cane molasses (UCM, 20g/L), respectively. After the UCM fed, the whole cell activity reached 5.2U/mL in a 7.5L fermentor. Optimum catalytic temperature and pH of whole cell were 35°C and 5...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28369693/a-bicistronic-vector-with-destabilized-mrna-secondary-structure-yields-scalable-higher-titer-expression-of-human-neurturin-in-e-coli
#8
Varnika Roy, Robert Roth, Mark Berge, Rajesh Chitta, Sucheta Vajrala, Srilatha Kuntumalla, Albert E Schmelzer, Ron Schoner
Human neurturin (NTN) is a cystine knot growth factor with potential therapeutic use in diseases such as Parkinson's and diabetes. Scalable high titer production of native NTN is particularly challenging because of the cystine knot structure which consists of an embedded ring comprised of at least three disulfide bonds. We sought to pursue enhanced scalable production of NTN in Escherichia coli. Our initial efforts focused on codon optimization of the first two codons following AUG, but these studies resulted in only a marginal increase in NTN expression...
March 28, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28365855/bioconversion-of-welan-gum-from-kitchen-waste-by-a-two-step-enzymatic-hydrolysis-pretreatment
#9
Panyu Li, Yi Xie, Yu Zeng, Wanrong Hu, Yan Kang, Xiang Li, Yabo Wang, Tonghui Xie, Yongkui Zhang
Kitchen waste (KW) is a worldwide issue, which can lead to environment pollution. Nevertheless, it is also a low-cost and sustainable resource for bio-production. Meanwhile, welan gum (WG) is one kind of the most important exopolysaccharide but with high material cost. The aim of this study was to adopt two-step enzymatic hydrolysis to improve the release and recovery of both sugar and protein in KW for subsequent WG production. As the results, the recovery rates of sugar and protein reached 81.07 and 77.38%, which were both satisfactory...
April 1, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28365562/responses-of-mixed-methanotrophic-consortia-to-variable-cu-2-fe-2-ratios
#10
Karthigeyan Chidambarampadmavathy, Obulisamy Parthiba Karthikeyan, Roger Huerlimann, Gregory E Maes, Kirsten Heimann
Methane mitigation in landfill top cover soils is mediated by methanotrophs whose optimal methane (CH4) oxidation capacity is governed by environmental and complex microbial community interactions. Optimization of CH4 remediating bio-filters need to take microbial responses into account. Divalent copper (Cu(2+)) and iron (Fe(2+)) are present in landfills at variable ratios and play a vital role in methane oxidation capacity and growth of methanotrophs. This study, as a first of its kind, therefore quantified effects of variable Cu(2+) and Fe(2+) (5:5, 5:25 and 5:50 μM) ratios on mixed methanotrophic communities enriched from landfill top cover (LB) and compost soils (CB)...
March 30, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28348799/community-acquired-urinary-tract-infections-caused-by-burkholderiacepacia-complex-in-patients-with-no-underlying-risk-factor
#11
Laila Nimri, Mamuno Sulaiman, Osama Bani Hani
Introduction. Urinary tract infections (UTIs) remain common infections diagnosed in outpatients as well as hospitalized patients. Community-acquired UTIs are generally caused by Escherichia coli and other members of the family Enterobacteriaceae.Burkholderiacepacia is an opportunistic pathogen mainly affecting immunocompromised and hospitalized patients, particularly those who have received prior broad-spectrum antibacterial therapy. Case presentation. Urine samples were collected from 157 outpatients clinically diagnosed with UTI and from 100 healthy control subjects...
January 2017: JMM Case Reports
https://www.readbyqxmd.com/read/28330311/scaling-up-a-virginiamycin-production-by-a-high-yield-streptomyces-virginiae-vkm-ac-2738d-strain-using-adsorbing-resin-addition-and-fed-batch-fermentation-under-controlled-conditions
#12
Vakhtang Dzhavakhiya, Vyacheslav Savushkin, Alexander Ovchinnikov, Vladislav Glagolev, Veronika Savelyeva, Evgeniya Popova, Nikita Novak, Elena Glagoleva
Virginiamycin produced by Streptomyces virginiae as a natural mix of macrocyclic peptidolactones M and S is widely used in the industrial production of ethanol fuel and as an antibiotic feed additive for cattle and poultry. Its main antimicrobial components, M1 and S1 factors, act synergistically if the M1:S1 ratio in the final product is 70-75:25-30. This fact significantly complicates the development of stable high-yield strains suitable for industrial application. In the previous work, authors obtained a mutant S...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330286/effect-of-highly-branched-hyphal-morphology-on-the-enhanced-production-of-cellulase-in-trichoderma-reesei-des-15
#13
Ronglin He, Chen Li, Lijuan Ma, Dongyuan Zhang, Shulin Chen
The morphology of Trichoderma reesei is a vitally important factor for cellulase productivity. This study investigated the effect of hyphal morphology on cellulase production in the hyper-cellulolytic mutant, T. reesei DES-15. With a distinct morphology, T. reesei DES-15 was obtained through Diethyl sulfite (DES) mutagenesis. The hyphal morphology of DES-15 batch-cultured in a 5-L fermentor was significantly shorter and more branched than the parental strain RUT C30. The cellulase production of DES-15 during batch fermentation was 66 % greater than that of RUT C30 when cultured the same conditions...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28322433/a-9-pool-metabolic-structured-kinetic-model-describing-days-to-seconds-dynamics-of-growth-and-product-formation-by-penicillium-chrysogenum
#14
Wenjun Tang, Amit T Deshmukh, Cees Haringa, Guan Wang, Walter van Gulik, Wouter van Winden, Matthias Reuss, Joseph J Heijnen, Jianye Xia, Ju Chu, Henk J Noorman
A powerful approach for the optimization of industrial bioprocesses is to perform detailed simulations integrating large scale computational fluid dynamics (CFD) and cellular reaction dynamics (CRD). However, complex metabolic kinetic models containing a large number of equations pose formidable challenges in CFD-CRD coupling and computation time afterward. This necessitates to formulate a relatively simple but yet representative model structure. Such a kinetic model should be able to reproduce metabolic responses for short-term (mixing time scale of tens of seconds) and long-term (fed-batch cultivation of hours/days) dynamics in industrial bioprocesses...
March 21, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28285222/efficient-production-of-lactulose-from-whey-powder-by-cellobiose-2-epimerase-in-an-enzymatic-membrane-reactor
#15
Lingtian Wu, Cen Xu, Sha Li, Jinfeng Liang, Hong Xu, Zheng Xu
In this study, the gene encoding cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus (CsCE) was successfully expressed in Bacillus subtilis WB800. After the fermentation medium optimization, the activity of recombinant strain was 4.5-fold higher than the original medium in a 7.5L fermentor. The optimal catalytic pH and temperature of crude CsCE were 7.0 and 80°C, respectively. An enzymatic synthesis of lactulose was developed using cheese-whey lactose as its substrate. The maximum conversion rate of whey powder obtained was 58...
March 2, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28278108/influence-of-al-3-on-the-titer-of-spiramycin-and-effective-components-in-fermentor
#16
Zhen Li, Fengxian Hu, Ruifang Ye, Heping Lv, Jun Zeng
Spiramycin is a multicomponent antibiotic, and different components have different antibacterial activities. In Streptomyces spiramyceticus 16-10-2, spiramycin II and spiramycin III (SPMII and SPMIII) are the main components, while spiramycin I (SPMI) needs to be controlled below 12%. Based on this, the influences of Al(3+) on total spiramycin titer and components were investigated in this work. Those experiments were mainly performed in 15 L fermentor and Al(3+) made a great improvement in spiramycin titer...
February 17, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28274103/expression-and-characterization-of-a-single-chain-variable-fragment-against-human-lox-1-in-escherichia-coli-and-brevibacillus-choshinensis
#17
Wei Hu, Jun-Yan Xiang, Ping Kong, Ling Liu, Qiuhong Xie, Hongyu Xiang
The single-chain variable fragment (scFv) against lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a promising molecule for its potential use in the diagnosis and immunotherapy of atherosclerosis. Producing this scFv in several milligram amounts could be the starting point for further engineering and application of the scFv. In this study, the abundant expression of the anti-LOX-1 scFv was attempted using Escherichia coli (E. coli) and Brevibacillus choshinensis (B. choshinensis). The scFv had limited soluble yield in E...
March 9, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28261538/efficient-expression-of-sortase-a-from-staphylococcus-aureus-in-escherichia-coli-and-its-enzymatic-characterizations
#18
Zhimeng Wu, Haofei Hong, Xinrui Zhao, Xun Wang
BACKGROUND: Sortase A (SrtA) is a transpeptidase found in Staphylococcus aureus, which is widely used in site-specific protein modification. However, SrtA was expressed in Escherichia coli (E. coli) in rather low level (ranging from several milligrams to 76.9 mg/L at most). The present study aims to optimize fermentation conditions for improving SrtA expression in E. coli. RESULTS: Under the optimized media (0.48 g/L glycerol, 1.37 g/L tryptone, 0.51 g/L yeast extract, MOPS 0...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28191448/a-food-grade-expression-system-for-d-psicose-3-epimerase-production-in-bacillus-subtilis-using-an-alanine-racemase-encoding-selection-marker
#19
Jingqi Chen, Zhaoxia Jin, Yuanming Gai, Jibin Sun, Dawei Zhang
BACKGROUND: Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expression system for d-psicose 3-epimerase production, we use an alanine racemase-encoding gene as selection marker in Bacillus subtilis. RESULTS: In this study, the d-alanine racemase-encoding gene dal was deleted from the chromosome of B. subtilis 1A751 using Cre/lox system to generate the food-grade host...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28186321/plasmid-encoded-biosynthetic-genes-alleviate-metabolic-disadvantages-while-increasing-glucose-conversion-to-shikimate-in-an-engineered-escherichia-coli-strain
#20
Alberto Rodriguez, Juan A Martínez, Pierre Millard, Guillermo Gosset, Jean-Charles Portais, Fabien Létisse, Francisco Bolivar
Metabolic engineering strategies applied over the last two decades to produce shikimate (SA) in Escherichia coli have resulted in a battery of strains bearing many expression systems. However, the effects that these systems have on the host physiology and how they impact the production of SA are still not well understood. In this work we utilized an engineered E. coli strain to determine the consequences of carrying a vector that promotes SA production from glucose with a high-yield but that is also expected to impose a significant cellular burden...
June 2017: Biotechnology and Bioengineering
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