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https://www.readbyqxmd.com/read/28932264/high-level-expression-and-characterization-of-a-novel-cutinase-from-malbranchea-cinnamomea-suitable-for-butyl-butyrate-production
#1
Xiaojie Duan, Yu Liu, Xin You, Zhengqiang Jiang, Shaoxiang Yang, Shaoqing Yang
BACKGROUND: Butyl butyrate has been considered as a promising fuel source because it is a kind of natural ester which can be converted from renewable and sustainable lignocellulosic biomass. Compared with the conventional chemical methods for butyl butyrate production, the enzymatic approach has been demonstrated to be more attractive, mainly owing to the mild reaction conditions, high specificity, low energy consumption, and environmental friendliness. Cutinases play an important role in the butyl butyrate production process...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28931206/metabolic-engineering-a-yeast-to-produce-astaxanthin
#2
Yu-Ju Lin, Jui-Jen Chang, Hao-Yeh Lin, Caroline Thia, Yi-Ying Kao, Chieh-Chen Huang, Wen-Hsiung Li
In this study, an astaxanthin-biosynthesis Kluyveromyces marxianus strain Sm23 was first constructed, which could produce 31µg/g DCW astaxanthin. Then, repeated genome integration of the key astaxanthin biosynthesis genes Hpchyb and bkt was done to increase gene copy number and astaxanthin yield. Four improved strains were obtained and the yield of astaxanthin and the total yield of carotenoids in a strain increased with the copy numbers of Hpchyb and bkt. To improve the yield further, the gene Hpchyb from Haematococcus pluvialis was modified by site-directed mutagenesis to increase the enzyme efficiency or/and to prevent the heterologous protein degradation by ubiquitination...
July 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28920400/-production-of-gastric-mucosa-protective-collagen-%C3%A2-by-pichia-pastoris
#3
Weina Li, Zifang Shang, Zhiguang Duan, Linbo Li, Jing He, Daidi Fan
To improve collagen production by recombinant Pichia pastoris, we applied Placket-Burman and Box-Behnken design to optimize the fermentation medium. Through Placket-Burman design, three variables in the medium (concentration of yeast extract, peptone and glycerol) were selected for having significant effect on cell dry weight. Through Box-Behnken design regression coefficients analysis, a secondary degree polynomial equation was established, and the optimum levels of the three variables were yeast extract 1...
April 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28912077/electron-receptor-addition-enhances-butanol-synthesis-in-abe-fermentation-by-clostridium-acetobutylicum
#4
Jian Ding, Hongzhen Luo, Fang Xie, Hao Wang, Meng Xu, Zhongping Shi
The techniques for enhancing butanol production in ABE fermentation by Clostridium acetobutylicum generally focus on adding electron carrier to strengthen NADH synthesis, repressing hydrogenase by aerating CO, supplementing butyrate, etc. However, those methods suffer from the problems of total solvent decrease, high purification cost, using expensive supplemental substances, etc. In this study, we added small amount of electron receptors (Na2SO4/CaSO4, 2g/L) into ABE fermentation broth: to alter electron/proton distributions in the intracellular electron transport shuttle system, directing more electron/proton pairs into NADH synthesis route; to stimulate intracellular accumulation of those amino acids favorable for cells survival/butanol synthesis...
September 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28876030/-overexpression-and-characterization-of-endo-cellulase-a10-from-aspergillus-nidulans-in-pichia-pastoris
#5
Jianjun Li, Xiaolian Li, Ziqiang Wang, Jianjun Li
Endo-cellulases are important to efficiently hydrolyze cellulose, and widely used in biotechnology. In this study, we overexpressed and characterized an endo-cellulase from Aspergillus nidulans. This endo-cellulase was successfully overexpressed in flasks and fermentor, and its concentration in fermentor reached 0.89 mg/mL. The optimal pH and temperature of the were 4.0 and 80 ℃ respectively, and it was very stable between pH 2.0 and 12.0. It was thermally stable below 60 ℃, whereas it was inactivated very quickly above 70 ℃...
May 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28861520/arginine-exposure-decreases-acidogenesis-in-long-term-oral-biofilm-microcosms
#6
Ruth G Ledder, Hitesh Mistry, Prem K Sreenivasan, Gavin Humphreys, Andrew J McBain
Arginine is an important pH-elevating agent in the oral cavity. It has been incorporated in oral hygiene formulations to mitigate sensitivity and to prevent caries. In this investigation, the effects of sustained arginine dosing of dental plaque microcosms on bacteriological composition and pH were evaluated under controlled conditions. Plaque microcosms were established in constant-depth film fermentors (CDFFs) using salivary inocula and fed continuously with artificial saliva. To simulate resting and cariogenic states, the CDFFs were supplemented with sterile water or 5% sucrose, respectively...
July 2017: MSphere
https://www.readbyqxmd.com/read/28831560/a-homofermentative-bacillus-sp-bc-001-and-its-performance-as-a-potential-l-lactate-industrial-strain
#7
Sitanan Thitiprasert, Kentaro Kodama, Somboon Tanasupawat, Phatthanon Prasitchoke, Tanapawarin Rampai, Budsabathip Prasirtsak, Vasana Tolieng, Jiraporn Piluk, Suttichai Assabumrungrat, Nuttha Thongchul
Bacillus sp. BC-001 was first reported as a potent thermotolerant and homofermentative strain for an industrial-scale L-lactate production. In a flask culture, this isolate fermented both glucose and sucrose to lactate with high yield (0.96 and 0.87 g/g) and productivity (2.8 and 2.6 g/L h), respectively. The higher lactate production performance was obtained in the simultaneous saccharification and fermentation of liquefied starch (150.1 g/L final titer, 0.98 g/g yield, 3.2 g/L h productivity) and the fed-batch glucose fermentation (139...
August 22, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28803103/engineering-halomonas-bluephagenesis-td01-for-non-sterile-production-of-poly-3-hydroxybutyrate-co-4-hydroxybutyrate
#8
Xiangbin Chen, Jin Yin, Jianwen Ye, Haoqian Zhang, Xuemei Che, Yiming Ma, Mengyi Li, Lin-Ping Wu, Guo-Qiang Chen
Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), short as P(3HB-co-4HB), was successfully produced by engineered Halomonas bluephagenesis TD01 grown in glucose and γ-butyrolactone under open non-sterile conditions. Gene orfZ encoding 4HB-CoA transferase of Clostridium kluyveri was integrated into the genome to achieve P(3HB-co-4HB) accumulation comparable to that of strains encoding orfZ on plasmids. Fed-batch cultivations conducted in 1-L and 7-L fermentors, respectively, resulted in over 70g/L cell dry weight (CDW) containing 63% P(3HB-co-12mol% 4HB) after 48h under non-sterile conditions...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28743657/vinegar-production-from-post-distillation-slurry-deriving-from-rice-shochu-production-with-the-addition-of-caproic-acid-producing-bacteria-consortium-and-lactic-acid-bacterium
#9
Hua-Wei Yuan, Li Tan, Hao Chen, Zhao-Yong Sun, Yue-Qin Tang, Kenji Kida
To establish a zero emission process, the post-distillation slurry of a new type of rice shochu (NTRS) was used for the production of health promoting vinegar. Since the NTRS post-distillation slurry contained caproic acid and lactic acid, the effect of these two organic acids on acetic acid fermentation was first evaluated. Based on these results, Acetobacter aceti CICC 21684 was selected as a suitable strain for subsequent production of vinegar. At the laboratory scale, acetic acid fermentation of the NTRS post-distillation slurry in batch mode resulted in an acetic acid concentration of 41...
July 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28723386/foam-adsorption-as-an-ex-situ-capture-step-for-surfactants-produced-by-fermentation
#10
Iva Anic, Arijit Nath, Pedro Franco, Rolf Wichmann
In this report, a method for a simultaneous production and separation of a microbially synthesized rhamnolipid biosurfactant is presented. During the aerobic cultivation of flagella-free Pseudomonas putida EM383 in a 3.1L stirred tank reactor on glucose as a sole carbon source, rhamnolipids are produced and excreted into the fermentation liquid. Here, a strategy for biosurfactant capture from rhamnolipid enriched fermentation foam using hydrophobic-hydrophobic interaction was investigated. Five adsorbents were tested independently for the application of this capture technique and the best performing adsorbent was tested in a fermentation process...
July 16, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28710630/scale-up-of-phytosterols-bioconversion-into-androstenedione
#11
Sonia Martínez-Cámara, Esther Bahíllo, José-Luis Barredo, Marta Rodríguez-Sáiz
Phytosterols, generated as a by-product of vegetable oils or wood pulp, contain the cyclopentane-perhydro-phenanthrene nucleus, and can be converted into steroid intermediates by removing the C17 side chain. This chapter shows the scale-up, from flask to fermentor, of the phytosterols bioconversion into 4-androstene-3,17-dione (androstenedione; AD) with Mycobacterium neoaurum B-3805. Due to the fact that phytosterols and AD are nearly insoluble in water, two-phase systems and the use of chemically modified cyclodextrins have been described as methods to solve it...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28708454/improving-the-production-of-s-adenosyl-l-methionine-in-escherichia-coli-by-overexpressing-metk
#12
Ping Yu, Pengzhi Zhu
S-adenosyl-L-methionine (SAM) has important applications in many fields including the chemical therapy and the pharmaceutical industry. In this study, the recombinant Escherichia coli strain was constructed for effective production of SAM by introducing the SAM synthase gene (metK). This strain produced 34.5 mg/l of SAM in basic medium in shake flask. Yeast extract, pH and loaded volume had a significant positive effect on the yield of SAM. Their optimal values were 35 g/l, 7.5 and 30 ml, respectively...
July 14, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28667643/upscale-production-of-a-recombinant-cyclodextrin-glycosyltransferase-from-paenibacillus-macerans-in-escherichia-coli
#13
Yi-Nan Yang, Wen-Xin Shan, Pi-Wu Wang
Cyclodextrin glucanotransferase (CGTase) is an important enzyme with multiple functions in starch utilization. In the present study, a fermentation process for the production of CGTase from Escherichia coli harboring the recombinant plasmid pET28b(+)-CGTase was investigated and optimized. The optimal fermentation and expression conditions were 10.0 g/L glycerol, 20.0 g/L tryptone, and 10.0 g/L yeast extract with an initial pH of 7.0, an IPTG concentration of 0.1 mM and an induction temperature of 28 °C for 10 h...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28656630/deleting-the-first-disulphide-bond-in-an-arenicin-derivative-enhances-its-expression-in-pichia-pastoris
#14
N Yang, X Wang, D Teng, R Mao, Y Hao, X Feng, J Wang
The marine antimicrobial peptide NZ17074, a variant of arenicin-3 from Arenicola marina that has broad antimicrobial activity and high bioavailability, can be designed to treat bacterial and fungal diseases. To reduce the toxicity of NZ17074, N6 was designed by replacing a cysteine in positions 3 and 20 with alanine, fused to the C-terminus of the small ubiquitin-like modifier tag (SUMO), and expressed in yeast. SUMO-N6 yielded as much as 921 mg l(-1) at 72 h after induction in a fermentor and increased 1·8-fold over SUMO-NZ17074...
June 28, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28616451/dataset-on-potential-large-scale-production-of-biosurfactant-using-bacillus-sp
#15
Hesty Heryani, Meilana Dharma Putra
Surfactants are very important in industry. The cost of commercial surfactant production is still high and the surfactant demand is constantly increasing. Microbial production of surfactant known as biosurfactant shows commercial potency. Utilization of Bacillus sp. strain on glucose fermentation for biosurfactant production was then studied. This type of microbe was isolated from soil contaminated with palm oil. The selection of the strain was based on its ability to form emulsifying zone around the colony and its capability to grow compared with those for commercial bacteria of Bacillus pumilus JCM 2508...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28612265/regular-enzyme-recovery-enhances-cellulase-production-by-trichoderma-reesei-in-fed-batch-culture
#16
Bucheng Wang, Lu Jiang, Hechao Bai, Qiang Yong, Shiyuan Yu
OBJECTIVE: To protect the enzymes during fed-batch cellulase production by means of partial enzyme recovery at regular intervals. RESULTS: Extracellular enzymes were partially recovered at the intervals of 1, 2, or 3 days. Mycelia were also removed to avoid contamination. Increases in the total harvested cellulase (24-62%) and β-glucosidase (22-76%) were achieved. In fermentor cultivation when the enzymes were recovered every day with 15% culture broth. The total harvested cellulase and β-glucosidase activity increased by 43 and 58%, respectively, with fungal cell concentration maintained at 3...
June 13, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28608479/biosynthesis-of-novel-7-8-dihydroxyflavone-glycoside-derivatives-and-in-silico-study-of-their-effects-on-bace1-inhibition
#17
Ramesh Prasad Pandey, Prakash Parajuli, Anaya Raj Pokhrel, Jae Kyung Sohng
7,8-Dihydroxyflavone (7,8-DHF) has been conjugated with glucose moiety to produce glucoside derivatives. Three analogues of 7,8-DHF (7-O-β-d-glucosyl-8-hydroxyflavone, 7-hydroxy-8-O-β-d-glucosyl flavone, and 7,8-di-O-β-d-glucosylflavone) have been successfully produced from in vitro reaction using glycosyltransferase of Bacillus licheniformis. Production of these 7,8-DHF derivatives were shifted to cheaper and easier approach in this study by using engineered Escherichia coli BL21 (DE3) ΔpgiΔzwfΔushA cells in which the flow of glucose-6-phospahte toward glycolysis and pentose phosphate pathway and hydrolysis of UDP-α-d-glucose were blocked while directing the carbon flux toward UDP-α-d-glucose by overexpressing UDP-α-d-glucose pathway genes...
June 12, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28593603/optimization-of-inexpensive-agricultural-by-products-as-raw-materials-for-bacitracin-production-in-bacillus-licheniformis-dw2
#18
Qin Wang, Hui Zheng, Xia Wan, Hongfeng Huang, Junhui Li, Christopher T Nomura, Changjun Wang, Shouwen Chen
Bacitracin is a broad-spectrum antibiotic used extensively as a feed additive. In this study, inexpensive agricultural by-products were used as nitrogen sources for bacitracin production. Based on both the orthogonal tests, a combination of 7% soybean meal (SBM) +2% low protein rapeseed cake (LPRC) was optimal for bacitracin production. Compared to the original formula, the titer of bacitracin increased by 20.5% reaching 910.4 U/ml in flasks. The titer of bacitracin and the ratio of bacitracin A increased by 12...
June 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28548573/inulin-type-fructan-degradation-capacity-of-clostridium-cluster-iv-and-xiva-butyrate-producing-colon-bacteria-and-their-associated-metabolic-outcomes
#19
F Moens, L De Vuyst
Four selected butyrate-producing colon bacterial strains belonging to Clostridium cluster IV (Butyricicoccus pullicaecorum DSM 23266(T) and Faecalibacterium prausnitzii DSM 17677(T)) and XIVa (Eubacterium hallii DSM 17630 and Eubacterium rectale CIP 105953(T)) were studied as to their capacity to degrade inulin-type fructans and concomitant metabolite production. Cultivation of these strains was performed in bottles and fermentors containing a modified medium for colon bacteria, including acetate, supplemented with either fructose, oligofructose, or inulin as the sole energy source...
May 30, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28484256/improved-thermostability-of-an-acidic-xylanase-from-aspergillus-sulphureus-by-combined-disulphide-bridge-introduction-and-proline-residue-substitution
#20
Wenhan Yang, Yongzhi Yang, Lingdi Zhang, Hang Xu, Xiaojing Guo, Xu Yang, Bing Dong, Yunhe Cao
As a feed additive, xylanase has been widely applied in the feed of monogastric animals, which contains multiple plant polysaccharides. However, during feed manufacture, the high pelleting temperatures challenge wild-type xylanases. The aim of this study was to improve the thermostability of Aspergillus sulphureus acidic xylanase. According to the predicted protein structure, a series of disulphide bridges and proline substitutions were created in the xylanase by PCR, and the mutants were expressed in Pichia pastoris...
May 8, 2017: Scientific Reports
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