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https://www.readbyqxmd.com/read/29925031/influence-of-pulsed-electric-fields-and-mitochondria-cytoskeleton-interactions-on-cell-respiration
#1
Ishan Goswami, Justin B Perry, Mitchell E Allen, David A Brown, Michael R von Spakovsky, Scott S Verbridge
Pulsed electric fields with microsecond pulse width (μsPEFs) are used clinically; namely, irreversible electroporation/Nanoknife is used for soft tissue tumor ablation. The μsPEF pulse parameters used in irreversible electroporation (0.5-1 kV/cm, 80-100 pulses, ∼100 μs each, 1 Hz frequency) may cause an internal field to develop within the cell because of the disruption of the outer cell membrane and subsequent penetration of the electric field. An internal field may disrupt voltage-sensitive mitochondria, although the research literature has been relatively unclear regarding whether such disruptions occur with μsPEFs...
June 19, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29924719/functional-and-patient-reported-outcomes-improve-over-the-course-of-rehabilitation-a-secondary-analysis-of-the-acl-sports-trial
#2
Amelia J H Arundale, Jacob J Capin, Ryan Zarzycki, Angela Smith, Lynn Snyder-Mackler
BACKGROUND: The Anterior Cruciate Ligament-Specialized Post-Operative Return to Sports (ACL-SPORTS) randomized controlled trial was designed to address deficits in functional and patient-reported outcomes. The trial examined the effects of a secondary ACL prevention program that included progressive strengthening, agility training, plyometrics (SAP), and other components of current primary prevention protocols, with perturbation training (SAP + PERT group) and without PERT (SAP group)...
June 1, 2018: Sports Health
https://www.readbyqxmd.com/read/29922590/a-pcr-based-quantitative-assay-for-the-evaluation-of-mrna-integrity-in-rat-samples
#3
Bhaja K Padhi, Manjeet Singh, Marianela Rosales, Guillaume Pelletier, Sabit Cakmak
Reverse Transcription quantitative real-time PCR (RT-qPCR) is applied to quantify gene transcript levels in a wide range of investigations. Proper assessment of RNA integrity is essential for reliable assessment of gene expression levels, as RNA molecules are acutely vulnerable to degradation. However, RNA quality control measures are still infrequently reported in rat toxicological studies, which impede proper evaluation of gene expression data reliability. The high operational cost of microfluidic capillary electrophoresis systems along with paucity of alternative methods for the quantitative assessment of rat RNA integrity constitute potential hurdles to the systematic implementation and reporting of RNA integrity assessment in rat studies...
May 2018: Biomolecular Detection and Quantification
https://www.readbyqxmd.com/read/29920474/the-short-chain-fatty-acids-and-lipopolysaccharides-status-in-sprague-dawley-rats-fed-with-high-fat-and-high-cholesterol-diet
#4
D Maciejewska, K Skonieczna-Zydecka, A Lukomska, I Gutowska, K Dec, P Kupnicka, J Palma, A Pilutin, W Marlicz, E Stachowska
Short chain fatty acids (SCFA) are produced by the gut microbiota during the fermentation of non-digestible polysaccharides. Diet is a major factor driving the composition and metabolism of the colonic microbiota. The aim of our study was to examine how a fat-rich and cholesterol-rich diet that, which leads to many metabolic disorders, affects the SCFA profile and lipopolysaccharide (LPS) concentration. The experiment was carried out on 72 male, 8-weeks-old Sprague-Dawley rats. The study group (n = 30 rats) received high-fat and high cholesterol diet (HFHCh)...
April 2018: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/29916182/a-novel-approach-to-enhance-acl-injury-prevention-programs
#5
EDITORIAL
Alli Gokeler, Romain Seil, Gino Kerkhoffs, Evert Verhagen
Efficacy studies have demonstrated decreased anterior cruciate ligament (ACL) injury rates for athletes participating in injury prevention programs. Typically, ACL injury prevention programs entail a combination of plyometrics, strength training, agility and balance exercises. Unfortunately, improvements of movement patterns are not sustained over time. The reason may be related to the type of instructions given during training. Encouraging athletes to consciously control knee movements during exercises may not be optimal for the acquisition of complex motor skills as needed in complex sports environments...
June 18, 2018: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/29913097/age-and-maturity-related-differences-in-motor-coordination-among-male-elite-youth-soccer-players
#6
Nikki Rommers, Mireille Mostaert, Lennert Goossens, Roel Vaeyens, Erik Witvrouw, Matthieu Lenoir, Eva D'Hondt
This study investigated differences in generic and soccer specific motor coordination, as well as speed and agility depending on age and maturity in elite youth soccer players (U10-U15, N = 619). Measurements included body height, body weight and sitting height to estimate age at peak height velocity (APHV); three Körperkoordinationstest für Kinder subtests (i.e. jumping sideways (JS), moving sideways (MS), balancing backwards (BB)) to assess generic motor coordination; the UGent dribbling test for soccer specific motor coordination; a 5m/30m sprint and T-test for speed and agility, respectively...
June 18, 2018: Journal of Sports Sciences
https://www.readbyqxmd.com/read/29912440/predictors-of-wellness-behaviors-in-u-s-army-physicians
#7
Daniel P Hsu, Shana L Hansen, Timothy A Roberts, Clinton K Murray, Vincent Mysliwiec
Introduction: In 2013, the U.S. Army Surgeon General implemented the Performance Triad (P3), an educational initiative to improve health-related behaviors of soldiers throughout the U.S. Army. The components of P3 are Sleep, Activity, and Nutrition with tenet behaviors for each component. This study reports the results of the 2015 U.S. Army Medical Corps survey regarding physician knowledge and adherence to the tenet behaviors of P3. Methods: In 2015, an anonymous survey was sent to all active duty U...
April 18, 2018: Military Medicine
https://www.readbyqxmd.com/read/29912081/combined-effects-of-self-myofascial-release-and-dynamic-stretching-on-range-of-motion-jump-sprint-and-agility-performance
#8
Erick D Richman, Brian M Tyo, Clayton R Nicks
Richman, ED, Tyo, BM, and Nicks, CR. Combined effects of self-myofascial release and dynamic stretching on range of motion, jump, sprint, and agility performance. J Strength Cond Res XX(X): 000-000, 2018-Massage has been used as both a pre- and post-exercise modality with purported benefits to flexibility and athletic performance. This study was designed to determine the effect of a 6-minute protocol of self massage known as self-myofascial release (SMR) using a foam rolling device in conjunction with a general warm-up and sport-specific dynamic stretching (DS) session on flexibility and explosive athletic performance in a sample of 14 female collegiate athletes...
June 14, 2018: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29911496/multicomponent-training-program-with-high-speed-movement-execution-of-ankle-muscles-reduce-risk-of-falls-in-older-adults
#9
Roberta Castilhos Detanico Bohrer, Gleber Pereira, Joice Katiane Mendes Beck, Angélica Lodovico, André Rodacki
The purpose of this study was to investigate the effects of multicomponent training program, designed to improve the torque around the ankle joint performing high-speed movement execution, on healthy older adults. Participants were balanced by torque around the ankle joint and randomly allocated to either exercise (n=12, 69.7±4.8 years, 74.6±16.8 kg, 1.63±0.10 m) or control group (n=14, 70.86±6.48 years; 73.5±13.4 kg, 1.56±0.05 m). The exercise group performed a multicomponent training of resistance, agility and coordination exercises, focusing on the plantar flexor muscles during 12 weeks (3 days per week)...
June 18, 2018: Rejuvenation Research
https://www.readbyqxmd.com/read/29910347/non-linear-resistance-training-program-induced-power-and-strength-but-not-linear-sprint-velocity-and-agility-gains-in-young-soccer-players
#10
Matheus Barbalho, Paulo Gentil, Rodolfo Raiol, Fabrício Boscolo Del Vecchio, Rodrigo Ramirez-Campillo, Victor Silveira Coswig
Background: The present study evaluated the effects of resistance training (RT) following a non-linear periodization model in the physical fitness of young soccer athletes. Methods: Young soccer players ( n = 23) were allocated into two groups: an RT group (RTG), and the control group (CON). The RTG underwent 15 weeks of non-linear RT periodization in three weekly sessions in addition to their specific soccer training. The CON continued performing the specific soccer training. Before and after the training period, all of the subjects performed one-repetition maximum (RM) tests for speed, agility, and power (vertical and horizontal jump)...
May 14, 2018: Sports
https://www.readbyqxmd.com/read/29910318/seasonal-variations-in-physical-fitness-and-performance-indices-of-elite-soccer-players
#11
Yoav Meckel, Ofer Doron, Eyal Eliakim, Alon Eliakim
The aim of the study was to investigate seasonal variations in fitness and performance indices of professional male soccer players. Eighteen professional male soccer players (age range 22⁻32 years) completed three similar sets of tests at three stages of the season: before preseason; after preseason and the middle of the competitive in-season. A significant decrease in body mass and percent fat was found during the preseason. A significant improvement ( p < 0.05) was found in the vertical jump (preseason: 37...
February 13, 2018: Sports
https://www.readbyqxmd.com/read/29908469/simultaneous-determination-of-six-bioactive-saponins-from-rhizoma-panacis-japonici-in-rat-plasma-by-uhplc-ms-ms-application-to-a-pharmacokinetic-study
#12
Hong Zheng, Feng Qiu, Hui Zhao, Jie Chen, Lei Wang, Haiyan Zou
A specific, sensitive and rapid ultra high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS) method was developed and validated for simultaneous determination of six major bioactive constituents in Rhizoma Panacis Japonici (RPJ), including oleanolic acid-type chikusetsusaponin V, IV, hemsgiganoside B, damarane-type ginsenoside Rb1, Rg1 and Re in rat plasma, using estazolam as the internal standard (IS). Plasma samples were pretreated with methanol/acetonitrile (1:1, V/V) for protein precipitation...
June 7, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29906417/selection-and-application-of-tissue-micrornas-for-non-endoscopic-diagnosis-of-barrett-s-esophagus
#13
Xiaodun Li, Sam Kleeman, Sally B Coburn, Carlo Fumagalli, Juliane Perner, Sriganesh Jammula, Ruth M Pfeiffer, Linda Orzolek, Haiping Hao, Philip R Taylor, Ahmad Miremadi, Núria Galeano-Dalmau, Pierre Lao-Sirieix, Maria Tennyson, Shona MacRae, Michael B Cook, Rebecca C Fitzgerald
BACKGROUND & AIMS: MicroRNA (miRNA) is highly stable in biospecimens and provides tissue-specific profiles, making it a useful biomarker of carcinogenesis. We aimed to discover a set of miRNAs that could accurately discriminate Barrett's esophagus (BE) from normal esophageal tissue and to test its diagnostic accuracy when applied to samples collected by a non-invasive esophageal cell sampling device. METHODS: We analyzed miRNA expression profiles of 2 independent sets of esophageal biopsy tissues collected during endoscopy from 38 patients with BE and 26 patients with normal esophagus (controls) using Agilent microarray and Nanostring counter assays...
June 12, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29905845/a-lc-ms-ms-assay-for-simultaneous-determination-of-two-glycopeptides-and-two-small-molecule-compounds-in-human-plasma
#14
Meiyun Shi, Xiaojun Zhao, Tingting Wang, Lei Yin, Yanyan Li
In this study, a novel and high-throughput liquid chromatography-tandem mass spectrometric (LC-MS-MS) assay was developed and validated for simultaneous determination of two glycopeptides (vancomycin, teicoplanin) and two small molecule compounds (meropenem, voriconazole) in human plasma. Only 50 μL of human plasma is used to quantify these four drugs simultaneously at clinical concentration levels. After a relative simple protein precipitation, the supernatant was then diluted with mobile phase acetonitrile: 0...
June 14, 2018: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/29903981/a-distributed-model-for-stressors-monitoring-based-on-environmental-smart-sensors
#15
Alberto de Ramón-Fernández, Daniel Ruiz-Fernández, Diego Marcos-Jorquera, Virgilio Gilart-Iglesias
Nowadays, in many countries, stress is becoming a problem that increasingly affects the health of people. Suffering stress continuously can lead to serious behavioral disorders such as anxiety or depression. Every person, in his daily routine, can face many factors which can contribute to increase his stress level. This paper describes a flexible and distributed model to monitor environmental variables associated with stress, which provides adaptability to any environment in an agile way. This model was designed to transform stress environmental variables in value added information (key stress indicator) and to provide it to external systems, in both proactive and reactive mode...
June 14, 2018: Sensors
https://www.readbyqxmd.com/read/29903209/-simultaneous-determination-of-6-kinds-of-food-additives-in-drink-by-hplc-ms-ms
#16
Hua Yin
OBJECTIVE: To establish a high performance liquid chromatographytandem mass spectrometry( HPLC-MS/MS) method for simultaneous determination of caffeine, benzoic acid, sorbic acid, acesulfame potassium, saccharin sodium and sodium cyclamate in drink. METHODS: The sample was extracted by ASPEC using extraction column( Pro Elut C_(18)500 mg/6 m L), dried by Turbo VapⅡ and dissolved in mobile phase. The supernatant was separated on Agilent SB-C_(18) column( 2. 1 mm × 50 mm, 1...
November 2017: Wei Sheng Yan Jiu, Journal of Hygiene Research
https://www.readbyqxmd.com/read/29899371/a-heterozygous-microdeletion-of-20q13-13-encompassing-adnp-gene-in-a-child-with-helsmoortel-van-der-aa-syndrome
#17
Minh-Tuan Huynh, Elise Boudry-Labis, Alfred Massard, Caroline Thuillier, Bruno Delobel, Bénédicte Duban-Bedu, Catherine Vincent-Delorme
Helsmoortel-van der Aa (SWI/SNF autism-related or ADNP syndrome) is an autosomal dominant monogenic syndrome caused by de novo variants in the last exon of ADNP gene and no deletions have been documented to date. We report the first case of a 3 years and 10 months old boy exhibiting typical features of ADNP syndrome, including intellectual disability, autistic traits, facial dysmorphism, hyperlaxity, mood disorder, behavioral problems, and severe chronic constipation. 60K Agilent array-comparative genomic hybridization (CGH) identified a heterozygous interstitial microdeletion at 20q13...
June 13, 2018: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/29897972/examination-of-the-perceived-agility-and-balance-during-a-reactive-agility-task
#18
Leia Stirling, Chika Eke, Stephen M Cain
In vehicle dynamics, it is commonly understood that there is an inverse relationship between stability and maneuverability. However, animal studies have found that stability and maneuverability can coincide. In this study, we examine humans running a reactive agility obstacle and consider the relationship between observational perceived agility and balance, as well as the relationship between quantified surrogates of agility and balance. Recreational athletes (n = 18) completed the agility task while wearing inertial measurement units (IMUs) on their body...
2018: PloS One
https://www.readbyqxmd.com/read/29897958/the-microrna-expression-signature-of-cd4-t-cells-in-the-transition-of-brucellosis-into-chronicity
#19
Ferah Budak, Salih Haldun Bal, Gulcin Tezcan, Emin Halis Akalın, Abdullah Yılmaz, Pınar Hız, Haluk Barbaros Oral
Brucellosis is a serious infectious disease that continues to be a significant cause of morbidity worldwide and across all ages. Despite early diagnosis and treatment, 10-30% of patients develop chronic brucellosis. Although there have been recent advances in our knowledge of Brucella virulence factors and hosts' immune response to the infection, there is a lack of clear data regarding how the infection bypasses the immune system and becomes chronic. The present study investigated immunological factors and their roles in the transition of brucellosis from an acute to a chronic infection in CD4+ T cells...
2018: PloS One
https://www.readbyqxmd.com/read/29897461/gdnf-induced-down-regulation-of-mir-145-5p-enhances-human-oocyte-maturation-and-cumulus-cell-viability
#20
Long Cui, Li Fang, Xiaodan Mao, Hsun-Ming Chang, Peter C K Leung, Yinghui Ye
Context: Although glial cell line-derived neurotrophic factor (GDNF) and microRNAs (miRNAs) have been shown to regulate mammalian oocyte maturation, little is known about their effects on human oocyte maturation and the underlying molecular mechanisms. Objectives: To examine the effects of GDNF on both nuclear and cytoplasmic maturation in cultured immature human oocytes and to investigate the involvement of miRNAs in GDNF-induced oocyte maturation. Design: A total of 200 human immature oocytes were used to evaluate the effects of GDNF on oocyte maturation...
April 20, 2018: Journal of Clinical Endocrinology and Metabolism
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