keyword
MENU ▼
Read by QxMD icon Read
search

biotechnology

keyword
https://www.readbyqxmd.com/read/27920772/streptococcus-thermophilus-apc151-strain-is-suitable-for-the-manufacture-of-naturally-gaba-enriched-bioactive-yogurt
#1
Daniel M Linares, Tom F O'Callaghan, Paula M O'Connor, R P Ross, Catherine Stanton
Consumer interest in health-promoting food products is a major driving force for the increasing global demand of functional (probiotic) dairy foods. Yogurt is considered the ideal medium for delivery of beneficial functional ingredients. Gamma-amino-butyric acid has potential as a bioactive ingredient in functional foods due to its health-promoting properties as an anti-stress, anti-hypertensive, and anti-diabetic agent. Here, we report the use of a novel Streptococcus thermophilus strain, isolated from the digestive tract of fish, for production of yogurt naturally enriched with 2 mg/ml of gamma-amino-butyric acid (200 mg in a standard yogurt volume of 100 ml), a dose in the same range as that provided by some commercially available gamma-amino-butyric acid supplements...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27919800/increasing-the-fidelity-of-noncanonical-amino-acid-incorporation-in-cell-free-protein-synthesis
#2
Qinglei Gan, Chenguang Fan
BACKGROUND: Cell-free protein synthesis provides a robust platform for co-translational incorporation of noncanonical amino acid (ncAA) into proteins to facilitate biological studies and biotechnological applications. Recently, eliminating the activity of release factor 1 has been shown to increase ncAA incorporation in response to amber codons. However, this approach could promote mis-incorporation of canonical amino acids by near cognate suppression. METHODS: We performed a facile protocol to remove near cognate tRNA isoacceptors of the amber codon from total tRNAs, and used the phosphoserine (Sep) incorporation system as validation...
December 2, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27919690/comparative-analysis-of-different-xanthan-samples-by-atomic-force-microscopy
#3
Julia Teckentrup, Orooba Al-Hammood, Tim Steffens, Hanna Bednarz, Volker Walhorn, Karsten Niehaus, Dario Anselmetti
The polysaccharide xanthan which is produced by the γ-proteobacterium Xanthomonas campestris is used as a food thickening agent and rheologic modifier in numerous food, cosmetics and technical applications. Its great commercial importance stimulated biotechnological approaches to optimize the xanthan production. By targeted genetic modification the metabolism of Xanthomonas can be modified in such a way that the xanthan production efficiency and/or the shear-thickening potency is optimized. Using atomic force microscopy (AFM) the secondary structure of single xanthan polymers produced by the wild type Xanthomonas campestris B100 and several genetically modified variations were analyzed...
December 2, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27917922/turning-diamagnetic-microbes-into-multinary-micro-magnets-magnetophoresis-and-spatio-temporal-manipulation-of-individual-living-cells
#4
Hojae Lee, Daewha Hong, Hyeoncheol Cho, Ji Yup Kim, Ji Hun Park, Sang Hee Lee, Ho Min Kim, Rawil F Fakhrullin, Insung S Choi
Inspired by the biogenic magnetism found in certain organisms, such as magnetotactic bacteria, magnetic nanomaterials have been integrated into living cells for bioorthogonal, magnetic manipulation of the cells. However, magnetized cells have so far been reported to be only binary system (on/off) without any control of magnetization degree, limiting their applications typically to the simple accumulation or separation of cells as a whole. In this work, the magnetization degree is tightly controlled, leading to the generation of multiple subgroups of the magnetized cells, and each subgroup is manipulated independently from the other subgroups in the pool of heterogeneous cell-mixtures...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27917165/the-chromobacterium-violaceum-arsr-arsenite-repressor-exerts-tighter-control-on-its-cognate-promoter-than-the-escherichia-coli-system
#5
Letícia M Arruda, Lummy M O Monteiro, Rafael Silva-Rocha
Environmental bacteria are endowed with several regulatory systems that have potential applications in biotechnology. In this report, we characterize the arsenic biosensing features of the ars response system from Chromobacterium violaceum in the heterologous host Escherichia coli. We show that the native Pars/arsR system of C. violaceum outperforms the chromosomal ars copy of E. coli when exposed to micromolar concentrations of arsenite. To understand the molecular basis of this phenomenon, we analyzed the interaction between ArsR regulators and their promoter target sites as well as induction of the system at saturating concentrations of the regulators...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27916886/cyanobacteria-as-chassis-for-industrial-biotechnology-progress-and-prospects
#6
REVIEW
Lamya Al-Haj, Yuen Tin Lui, Raeid M M Abed, Mohamed A Gomaa, Saul Purton
Cyanobacteria hold significant potential as industrial biotechnology (IB) platforms for the production of a wide variety of bio-products ranging from biofuels such as hydrogen, alcohols and isoprenoids, to high-value bioactive and recombinant proteins. Underpinning this technology, are the recent advances in cyanobacterial "omics" research, the development of improved genetic engineering tools for key species, and the emerging field of cyanobacterial synthetic biology. These approaches enabled the development of elaborate metabolic engineering programs aimed at creating designer strains tailored for different IB applications...
November 30, 2016: Life
https://www.readbyqxmd.com/read/27916599/deciphering-communicating-and-engineering-the-crispr-pam
#7
REVIEW
Ryan T Leenay, Chase L Beisel
Clustered regularly interspaced short palindromic repeat (CRISPR) loci and their flanking CRISPR-associated (cas) genes make up RNA-guided, adaptive immune systems in prokaryotes whose effector proteins have become powerful tools for basic research and biotechnology. While the Cas effector proteins are remarkably diverse, they commonly rely on protospacer-adjacent motifs (PAMs) as the first step in target recognition. PAM sequences are known to vary considerably between systems and have proven to be difficult to predict, spurring the need for new tools to rapidly identify and communicate these sequences...
December 1, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27916552/plenty-is-no-plague-streptomyces-symbiosis-with-crops
#8
REVIEW
Thomas Rey, Bernard Dumas
Streptomyces spp. constitute a major clade of the phylum Actinobacteria. These Gram-positive, filamentous prokaryotes are ubiquitous in soils and marine sediments, and are commonly found in the rhizosphere or inside plant roots. Plant-interacting Streptomyces have received limited attention, in contrast to Streptomyces spp. extensively investigated for decades in medicine given their rich potential for secondary metabolite biosynthesis. Recent genomic, metabolomic, and biotechnological advances have produced key insights into Streptomyces spp...
November 5, 2016: Trends in Plant Science
https://www.readbyqxmd.com/read/27915977/a-review-on-recent-patents-and-applications-of-inorganic-material-binding-peptides
#9
Veeranjaneyulu Thota, Carole C Perry
Over the past decade, significant progress has been made in the identification of novel material binding peptides having affinity to a wide range of target materials and their use in nanobiotechnological innovations. These material binding peptides (MBPs), also known as solid/ substance binding peptides (SBPs) can be isolated using combinatorial display technologies such as phage display (PD), surface display (cell, bacterial, yeast, mRNA) exhibit material specific selectivity and affinity towards a range of inorganic and organic nanomaterial surfaces including metals, metal oxides, minerals, semiconductors and biomolecules...
December 1, 2016: Recent Patents on Nanotechnology
https://www.readbyqxmd.com/read/27915455/overexpression-of-atedt1-promotes-root-elongation-and-affects-medicinal-secondary-metabolite-biosynthesis-in-roots-of-transgenic-salvia-miltiorrhiza
#10
Yu Liu, Geng Sun, Zhaohui Zhong, Linyi Ji, Yong Zhang, Jianping Zhou, Xuelian Zheng, Kejun Deng
Medicinal secondary metabolites (salvianolic acids and tanshinones) are valuable natural bioactive compounds in Salvia miltiorrhiza and have widespread applications. Improvement of medicinal secondary metabolite accumulation through biotechnology is necessary and urgent to satisfy their increasing demand. Herein, it was demonstrated that the overexpression of the transcription factor Arabidopsis thaliana-enhanced drought tolerance 1 (AtEDT1) could affect medicinal secondary metabolite accumulation. In this study, we observed that the transgenic lines significantly conferred drought tolerance phenotype...
December 3, 2016: Protoplasma
https://www.readbyqxmd.com/read/27915377/crystal-structure-and-characterization-of-esterase-est25-mutants-reveal-improved-enantioselectivity-toward-s-ketoprofen-ethyl-ester
#11
Jinyeong Kim, Seung-Hyeon Seok, Eunsoo Hong, Tae Hyeon Yoo, Min-Duk Seo, Yeonwoo Ryu
Esterases comprise a group of enzymes that catalyze the cleavage and synthesis of ester bonds. They are important in biotechnological applications owing to their enantioselectivity, regioselectivity, broad substrate specificity, and the fact that they do not require cofactors. In a previous study, we isolated the esterase Est25 from a metagenomic library. Est25 showed catalytic activity toward the (R,S)-ketoprofen ethyl ester but had low enantioselectivity toward the (S)-ketoprofen ethyl ester. Because (S)-ketoprofen has stronger anti-inflammatory effects and fewer side effects than (R)-ketoprofen, enantioselectivity of this esterase is important...
December 3, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27915147/highly-sensitive-optical-interferometric-technique-reveals-stress-dependent-instantaneous-nanometric-growth-fluctuations-of-chinese-chive-leaf-under-heavy-metal-stress
#12
Kokge Thilini Kanchana Muthumali DeSilva, Uma Maheswari Rajagopalan, Hirofumi Kadono
Plant growth apart from being a complex and highly dynamic is dependent on its immediate environment. Leaf expansion measurements using Statistical Interferometry Technique, a sensitive interferometric technique at nanometric accuracy and at sub-second levels revealed the presence of characteristic nanometric intrinsic fluctuations [Plant Biotechnology 31, 195 (2014)]. In this paper, we demonstrate that the nanometric intrinsic fluctuations are sensitive enough that they change under exposure of heavy metals, essential micronutrient zinc and non-essential element cadmium, at relatively low concentrations in the leaves of Chinese chive (Allium tuberosum)...
December 1, 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27915100/synergistic-effect-of-biogenic-fe-3-coupled-to-s%C3%A2-oxidation-on-simultaneous-bioleaching-of-cu-co-zn-and-as-from-hazardous-pyrite-ash-waste
#13
Sandeep Panda, Ata Akcil, Srabani Mishra, Ceren Erust
Pyrite ash, a waste by-product formed during roasting of pyrite ores, is a good source of valuable metals. The waste is associated with several environmental issues due to its dumping in sea and/or land filling. Although several other management practices are available for its utilization, the waste still awaits and calls for an eco-friendly biotechnological application for metal recovery. In the present study, chemolithotrophic meso-acidophilic iron and sulphur oxidisers were evaluated for the first time towards simultaneous mutli-metal recovery from pyrite ash...
November 19, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27915060/special-issue-of-new-biotechnology-in-memory-of-professor-brian-f-c-clark-1936-2014
#14
EDITORIAL
Francesco Lescai
No abstract text is available yet for this article.
November 30, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27914332/amyloid-graphene-oxide-as-immobilization-platform-of-au-nanocatalysts-and-enzymes-for-improved-glucose-sensing-activity
#15
Xiaochen Wu, Mingjie Li, Zehui Li, Lili Lv, Yan Zhang, Chaoxu Li
Two-dimensional GO nanosheets and one-dimensional lysozyme nanofibrils were hybridized through electrostatic interaction to get a novel amyloid-GO composite, which promised a biocompatible immobilization platform for Au nanocatalysts as well as enzymes. The immobilization platform could load a large and tunable amount of Au NPs while maintaining their high catalytic activity. The immobilized catalysts showed high electrochemical behaviors, being ideal as glucose sensing systems. Furthermore, enzymes could also be immobilized on the residual bare surfaces of amyloid-GO, and served by a colorimetric method for a sensitive and selective analytical glucose-detecting platform...
November 16, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27914061/probing-oligomerized-conformations-of-defensin-in-the-membrane
#16
Wenxun Gan, Dina Schneidman, Ning Zhang, Buyong Ma, Ruth Nussinov
Computational prediction and design of membrane protein-protein interactions facilitate biomedical engineering and biotechnological applications. Due to their antimicrobial activity, human defensins play an important role in the innate immune system. Human defensins are attractive pharmaceutical targets due to their small size, broad activity spectrum, reduced immunogenicity, and resistance to proteolysis. Protein engineering based modification of defensins can improve their pharmaceutical properties. Here we present an approach to computationally probe defensins' oligomerization states in the membrane...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27912244/environmental-sustainability-analysis-and-nutritional-strategies-of-animal-production-in-china
#17
Bie Tan, Yulong Yin
Animal production in China has achieved considerable progress and contributes to46%of the total agriculture output value of the country. However, this fast expansion of animal production has led to environmental pollution. In this article, we review the status of soil, water, and air pollution associated with animal production in China and analyze the main sources of the pollutants. The government has promulgated regulations and standards, and effective models and technologies have been developed to control pollution during the last 10 years...
November 21, 2016: Annual Review of Animal Biosciences
https://www.readbyqxmd.com/read/27910966/stem-cell-and-regenerative-medicine-global-conference-scrgc-2016-august-23-24-2016-gyeonggi-do-korea
#18
A Vertès
In its third edition, the Stem Cell and Regenerative Medicine Global Conference (SCRGC) organized by the Global Stem Cell & Regenerative Medicine Acceleration Center (GSRAC) was focused on breaking barriers to accelerate the pace of innovation and development of the regenerative medicine industry. GSRAC is both a think tank and a global network of key opinion leaders from the public and the private sectors. GSRAC was commissioned in 2011 by the Ministry of Health and Welfare (MOHW) of Korea. GSRAC's primary mission is to enable and accelerate the delivery of innovative technologies to patients who are affected by currently untreatable diseases...
October 2016: Drugs of Today
https://www.readbyqxmd.com/read/27910910/genome-wide-characterisation-and-expression-profile-of-the-grapevine-atl-ubiquitin-ligase-family-reveal-biotic-and-abiotic-stress-responsive-and-development-related-members
#19
Pietro Ariani, Alice Regaiolo, Arianna Lovato, Alejandro Giorgetti, Andrea Porceddu, Salvatore Camiolo, Darren Wong, Simone Castellarin, Elodie Vandelle, Annalisa Polverari
The Arabidopsis Tóxicos en Levadura (ATL) protein family is a class of E3 ubiquitin ligases with a characteristic RING-H2 Zn-finger structure that mediates diverse physiological processes and stress responses in plants. We carried out a genome-wide survey of grapevine (Vitis vinifera L.) ATL genes and retrieved 96 sequences containing the canonical ATL RING-H2 domain. We analysed their genomic organisation, gene structure and evolution, protein domains and phylogenetic relationships. Clustering revealed several clades, as already reported in Arabidopsis thaliana and rice (Oryza sativa), with an expanded subgroup of grapevine-specific genes...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910753/medicinal-value-and-taxonomy-of-the-tinder-polypore-fomes-fomentarius-agaricomycetes-a-review
#20
Ján Gáper, Svetlana Gáperová, Peter Pristas, Katerina Naplavova
The tinder polypore, Fomes fomentarius, is a wood-decaying macrofungus well known for its potential use in a wide range of biotechnological applications. The existence of 3 distinct internal transcribed spacer lineages/sublineages among its strains has been clearly established. Sublineage A1 consists of strains isolated from North America, whereas sublineage A2 consists of strains only from Europe. Lineage B comprises strains from Europe and Asia. A better understanding of the biological features of F. fomentarius lineages/sublineages could lead to improved characterization, leading to better biotechnological applications...
2016: International Journal of Medicinal Mushrooms
keyword
keyword
1770
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"