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https://www.readbyqxmd.com/read/29779183/molecular-characterization-and-potential-synthetic-applications-of-gh1-%C3%AE-glucosidase-from-higher-termite-microcerotermes-annandalei
#1
Siriphan Arthornthurasuk, Wantha Jenkhetkan, Eukote Suwan, Daranee Chokchaichamnankit, Chantragan Srisomsap, Pakorn Wattana-Amorn, Jisnuson Svasti, Prachumporn T Kongsaeree
A novel β-glucosidase from higher termite Microcerotermes annandalei (MaBG) was obtained via a screening method targeting β-glucosidases with increased activities in the presence of glucose. The purified natural MaBG showed a subunit molecular weight of 55 kDa and existed in a native form as a dimer without any glycosylation. Gene-specific primers designed from its partial amino acid sequences were used to amplify the corresponding 1,419-bp coding sequence of MaBG which encodes a 472-amino acid glycoside hydrolase family 1 (GH1) β-glucosidase...
May 19, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29778976/developing-two-reference-control-samples-for-the-indian-population
#2
Shruti Iyer, Priyanka Bhatia, Mahendra Rao, Odity Mukherjee
Human induced Pluripotent Stem Cells (HiPSCs) have immense potential in research and therapeutics. Under the aegis of Department of Biotechnology funded national program entitled, "The Accelerator program for Discovery in Brain Disorders using Stem Cells (ADBS)" we have established a HiPSC biorepository (https://www.ncbs.res.in/adbs/bio-repository) with an objective to study severe mental illness. The repository comprises of HiPSC lines derived from healthy control donors and individuals with life time diagnosis of severe mental illness from dense families...
May 12, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29778876/genome-sequence-analysis-of-a-novel-bacillus-thuringiensis-strain-blb406-active-against-aedes-aegypti-larvae-a-novel-potential-bioinsecticide
#3
Raida Zribi Zghal, Kais Ghedira, Jihen Elleuch, Marwa Kharrat, Slim Tounsi
BLB406 is a novel isolate of Bacillus thuringiensis with a larvicidal activity against Aedes aegypti larvae. It displays original plasmidic and crystal protein patterns. The present work reported molecular and bioinformatic analyses for the genome sequence of BLB406 using MiSeq Illumina next-generation sequencing technology. The reads were assembled by Velvet tool. Using RAST program and PGAAP the genome of BLB406 strain was shown to contain 6297 genes corresponding to 5924 protein coding sequences. The BLB406 genome investigation with BtToxin_scanner program shows that this strain has an original and different combination of toxins compared to the published ones: five cry genes (cry11, cry22, cry2, cry60, cry64) and two distinct vegetative insecticidal vip4 genes...
May 17, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29778531/solvent-tolerance-in-bacteria-fulfilling-the-promise-of-the-biotech-era
#4
REVIEW
Hadiastri Kusumawardhani, Rohola Hosseini, Johannes H de Winde
The challenge of sustainably producing highly valuable chemical compounds requires specialized microbial cell factories because many of these compounds can be toxic to microbial hosts. Therefore, solvent-tolerant bacteria are promising production hosts because of their intrinsic tolerance towards these compounds. Recent studies have helped to elucidate the molecular mechanisms involved in solvent tolerance. Advances in synthetic biological tools will enable further development of streamlined solvent-tolerant production hosts and the transfer of solvent-tolerant traits to established industrial strains...
May 16, 2018: Trends in Biotechnology
https://www.readbyqxmd.com/read/29778530/exploiting-bacteriophage-proteomes-the-hidden-biotechnological-potential
#5
REVIEW
Sílvio B Santos, Ana Rita Costa, Carla Carvalho, Franklin L Nóbrega, Joana Azeredo
Bacteriophages encode many distinct proteins for the successful infection of a bacterial host. Each protein plays a specific role in the phage replication cycle, from host recognition, through takeover of the host machinery, and up to cell lysis for progeny release. As the roles of these proteins are being revealed, more biotechnological applications can be anticipated. Phage-encoded proteins are now being explored for the control, detection, and typing of bacteria; as vehicles for drug delivery; and for vaccine development...
May 16, 2018: Trends in Biotechnology
https://www.readbyqxmd.com/read/29777738/establishment-of-an-efficient-genetic-transformation-method-in-dunaliella-tertiolecta-mediated-by-agrobacterium-tumefaciens
#6
Claudia D Norzagaray-Valenzuela, Lourdes J Germán-Báez, Marco A Valdez-Flores, Sergio Hernández-Verdugo, Luke M Shelton, Angel Valdez-Ortiz
Microalgae are photosynthetic microorganisms widely used for the production of highly valued compounds, and recently they have been shown to be promising as a system for the heterologous expression of proteins. Several transformation methods have been successfully developed, from which the Agrobacterium tumefaciens-mediated method remains the most promising. However, microalgae transformation efficiency by A. tumefaciens is shown to vary depending on several transformation conditions. The present study aimed to establish an efficient genetic transformation system in the green microalgae Dunaliella tertiolecta using the A...
May 16, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29776370/characterization-of-a-promiscuous-cadmium-and-arsenic-resistance-mechanism-in-thermus-thermophilus-hb27-and-potential-application-of-a-novel-bioreporter-system
#7
Immacolata Antonucci, Giovanni Gallo, Danila Limauro, Patrizia Contursi, Ana Luisa Ribeiro, Alba Blesa, José Berenguer, Simonetta Bartolucci, Gabriella Fiorentino
BACKGROUND: The characterization of the molecular determinants of metal resistance has potential biotechnological application in biosensing and bioremediation. In this context, the bacterium Thermus thermophilus HB27 is a metal tolerant thermophile containing a set of genes involved in arsenic resistance which, differently from other microbes, are not organized into a single operon. They encode the proteins: arsenate reductase, TtArsC, arsenic efflux membrane transporter, TtArsX, and transcriptional repressor, TtSmtB...
May 18, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29775749/a-novel-oncogene-urg4-urgcp-and-its-role-in-cancer
#8
REVIEW
Yavuz Dodurga, Mücahit Seçme, N Lale Şatıroğlu-Tufan
Oncogenes are mutated form of normal cellular genes called as proto-oncogenes and conduce to the cancer development process. Despite the fact that so many genes have been described, new genes with oncogenic characteristic and potential or tumor supressoring activity are still being defined. Recently, Up-regulated gene 4/Upregulator of cell proliferation (URG4/URGCP), a novel gene, induced by hepatitis-Bvirus-encoded X antigen (HBxAg), has been identified. URG4/URGCP gene was registered to the National Center for Biotechnology Information-GenBank (NCBI-GenBank, Entrez GeneID: 55665 and Entrez Nucleotide ID NM_017920)...
May 15, 2018: Gene
https://www.readbyqxmd.com/read/29775576/the-quest-for-stability-during-times-of-change
#9
Quincey Justman
Experimental tools that are designed to perturb biological functions are often used to understand how cells change over time. However, in two recent papers, Segall-Shapiro et al. (2018; in Nature Biotechnology) and Rullan et al. (2018; in this issue of Molecular Cell) present tools engineered to keep cells the same. Both tools achieve perfect adaptation, that is, the ability to hold a value, in this case gene expression, constant over time despite disruptions and disturbances. Together, these papers highlight how influential dynamics can be when shaping biological functions, even when the goal is to stay constant...
May 17, 2018: Molecular Cell
https://www.readbyqxmd.com/read/29773867/novel-haloarchaeon-natrinema-thermophila-having-the-highest-growth-temperature-among-haloarchaea-with-a-large-genome-size
#10
Yeon Bee Kim, Joon Yong Kim, Hye Seon Song, Changsu Lee, Seung Woo Ahn, Se Hee Lee, Min Young Jung, Jin-Kyu Rhee, Juseok Kim, Dong-Wook Hyun, Jin-Woo Bae, Seong Woon Roh
Environmental temperature is one of the most important factors for the growth and survival of microorganisms. Here we describe a novel extremely halophilic archaeon (haloarchaea) designated as strain CBA1119T isolated from solar salt. Strain CBA1119T had the highest maximum and optimal growth temperatures (66 °C and 55 °C, respectively) and one of the largest genome sizes among haloarchaea (5.1 Mb). It also had the largest number of strain-specific pan-genome orthologous groups and unique pathways among members of the genus Natrinema in the class Halobacteria...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29773775/case-study-in-international-cooperation-cuba-s-molecular-immunology-center-and-roswell-park-cancer-institute
#11
Rachel Evans, Mary Reid, Brahm Segal, Scott I Abrams, Kelvin Lee
In 1961, the USA severed diplomatic relations with Cuba, and in 1962 an embargo was imposed on trade and financial relations with that country. It was not until five decades later that the USA and Cuba would reestablish relations. This opened the way for the New York State Trade Mission to Cuba in April 2015, during which Cuba's Molecular Immunology Center and Buffalo, New York's Roswell Park Cancer Institute signed a formal agreement that would set in motion biotechnology research collaboration to address one of the most important causes of death in both countries...
April 2018: MEDICC Review
https://www.readbyqxmd.com/read/29773774/us-and-cuban-scientists-forge-collaboration-on-arbovirus-research
#12
Jorge Pérez-Ávila, Maria G Guzmán-Tirado, Jorge Fraga-Nodarse, Gray Handley, James Meegan, Jose L Pelegrino-Martínez de la Cotera, Anthony S Fauci
After December 17, 2014, when the US and Cuban governments announced their intent to restore relations, the two countries participated in various exchange activities in an effort to encourage cooperation in public health, health research and biomedical sciences. The conference entitled Exploring Opportunities for Arbovirus Research Collaboration, hosted at Havana's Hotel Nacional, was part of these efforts and was the first major US-Cuban scientific conference in over 50 years. Its purpose was to share information about current arbovirus research and recent findings, and to explore opportunities for future joint research...
April 2018: MEDICC Review
https://www.readbyqxmd.com/read/29772529/microbial-bio-fuels-a-solution-to-carbon-emissions-and-energy-crisis
#13
Arun Kumar, Sumit Kaushal, Shubhini A Saraf, Jay Shankar Singh
Increasing energy demand, limited fossil fuel resources and climate change have prompted development of alternative sustainable and economical fuel resources such as crop-based bio-ethanol and bio-diesel. However, there is concern over use of arable land that is used for food agriculture for creation of biofuel. Thus, there is a renewed interest in the use of microbes particularly microalgae for bio-fuel production. Microbes such as micro-algae and cyanobacteria that are used for biofuel production also produce other bioactive compounds under stressed conditions...
June 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/29771832/growth-factors-release-from-concentrated-growth-factors-effect-of-%C3%AE-tricalcium-phosphate-addition
#14
Veronica Bonazza, Christopher Hajistilly, Dinker Patel, Jatan Patel, Rita Woo, Marco Angelo Cocchi, Barbara Buffoli, Davide Lancini, Ezio Gheno, Rita Rezzani, Sadia Jahanzeb, Adrian Hunnisett, Maher Almasri, Alberta Greco Lucchina, Matteo Brucoli, Carmen Mortellaro, Luigi Fabrizio Rodella
BACKGROUND: Platelet concentrates represent a new approach to improve tissue regeneration and can be used alone or together with autogenous bone, recombinant human growth factors, and/or other biomaterials, to enhance tissue regeneration. Among platelet concentrates, concentrated growth factors (CGFs) exhibit an interesting clinical and biotechnological application potential. OBJECTIVE: The aim of this study was to evaluate the in vitro release of 4 growth factors (bone morphogenetic proteins [BMP] -2, BMP-7, transforming growth factor [TGF] -β1, and insulin-like growth factor [IGF] -1) by the enzyme-linked immunosorbent assay (ELISA) technique, in CGFs mixed or not with β-tricalcium phosphate (β-TCP), using or not the Round-up device, at different times...
May 15, 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29770369/nanomaterial-microbe-cross-talk-physicochemical-principles-and-patho-biological-consequences
#15
REVIEW
D Westmeier, A Hahlbrock, C Reinhardt, J Fröhlich-Nowoisky, S Wessler, C Vallet, U Pöschl, S K Knauer, R H Stauber
The applications of nanoparticles (NPs) are increasing exponentially in consumer products, biotechnology and biomedicine, and humans, as well as the environment, are increasingly being exposed to NPs. Analogously, various (pathogenic) microorganisms are present at all the major exposure and entry sites for NPs in the human body as well as in environmental habitats. However, the field has just started to explore the complex interplay between NPs and microbes and the (patho)biological consequences. Based on recent insights, herein, we critically reviewed the available knowledge about the interaction of NPs with microbes and the analytical investigations including the latest intravital imaging tools...
May 17, 2018: Chemical Society Reviews
https://www.readbyqxmd.com/read/29769587/explorations-on-the-ecological-role-of-toxin-secretion-and-delivery-in-jawless-predatory-polychaeta
#16
N Cuevas, M Martins, A P Rodrigo, C Martins, P M Costa
Motivated by biotechnological prospects, there is increasing evidence that we may just be scraping the tip of the iceberg of poisonous marine invertebrates, among which the Polychaeta are promising candidates for bioprospecting. Here we show that an inconspicuous phyllodocid uses toxins in its uncanny feeding strategy. The worm, a jawless active predator characterised by its bright green colour, preys on larger invertebrates (including conspecifics) by extracting tissue portions with its powerful proboscis through suction...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769552/a-comprehensive-bioinformatics-analysis-on-multiple-gene-expression-omnibus-datasets-of-nonalcoholic-fatty-liver-disease-and-nonalcoholic-steatohepatitis
#17
Shanzhou Huang, Chengjun Sun, Yuchen Hou, Yunhua Tang, Zebin Zhu, Zhiheng Zhang, Yixi Zhang, Linhe Wang, Qiang Zhao, Mao-Gen Chen, Zhiyong Guo, Dongping Wang, Weiqiang Ju, Qi Zhou, Linwei Wu, Xiaoshun He
Fatty liver disease is one of the leading causes of chronic damage in western countries. Approximately 25% of adults in the United States have fatty livers in the absence of excessive alcohol consumption, a condition termed nonalcoholic fatty liver disease (NAFLD). Little is known about the prevalence and genetic background of NAFLD or the factors that determine its development. In this study, we used the Gene-Cloud of Biotechnology Information bioinformatics platform to carry out a comprehensive bioinformatics analysis identifying differentially expressed genes (DEGs), key biological processes and intersecting pathways...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769325/the-ancient-phosphatidylinositol-3-kinase-signaling-system-is-a-master-regulator-of-energy-and-carbon-metabolism-in-algae
#18
Rishiram Ramanan, Quynh-Giao Tran, Dae-Hyun Cho, Jae-Eun Jeong, Byung-Hyuk Kim, Sang-Yoon Shin, Sae-Hae Choi, Kwang-Hyeon Liu, Dae-Soo Kim, Seon-Jin Lee, Jose L Crespo, Hee-Gu Lee, Hee-Mock Oh, Hee-Sik Kim
Algae undergo a complete metabolic transformation under stress by arresting cell growth, inducing autophagy, and hyper-accumulating biofuel precursors such as triacylglycerols and starch. However, the regulatory mechanisms behind this stress-induced transformation are still unclear. Here, we use biochemical, mutational, and 'omics' approaches to demonstrate that phosphatidylinositol 3-kinase (PI3K) signaling mediates the homeostasis of energy molecules and influences carbon metabolism in algae. In Chlamydomonas reinhardtii, the inhibition and knockdown (KD) of algal class III PI3K led to significantly decreased cell growth, altered cell morphology, and higher lipid and starch contents...
May 16, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29768936/nutrient-removal-and-biomass-production-advances-in-microalgal-biotechnology-for-wastewater-treatment
#19
Sudharsanam Abinandan, Suresh R Subashchandrabose, Kadiyala Venkateswarlu, Mallavarapu Megharaj
Owing to certain drawbacks, such as energy-intensive operations in conventional modes of wastewater treatment (WWT), there has been an extensive search for alternative strategies in treatment technology. Biological modes for treating wastewaters are one of the finest technologies in terms of economy and efficiency. An integrated biological approach with chemical flocculation is being conventionally practiced in several-sewage and effluent treatment plants around the world. Overwhelming responsiveness to treat wastewaters especially by using microalgae is due to their simplest photosynthetic mechanism and ease of acclimation to various habitats...
May 17, 2018: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29768221/optimised-synthesis-of-zno-nano-fertiliser-through-green-chemistry-boosted-growth-dynamics-of-economically-important-l-esculentum
#20
Nyla Jabeen, Qaisar Maqbool, Tahira Bibi, Mudassar Nazar, Syed Z Hussain, Talib Hussain, Tariq Jan, Ishaq Ahmad, Malik Maaza, Sadaf Anwaar
Mounting-up economic losses to annual crops yield due to micronutrient deficiency, fertiliser inefficiency and increasing microbial invasions (e.g. Xanthomonas cempestri attack on tomatoes) are needed to be solved via nano-biotechnology. So keeping this in view, the authors' current study presents the new horizon in the field of nano-fertiliser with highly nutritive and preservative effect of green fabricated zinc oxide-nanostructures (ZnO-NSs) during Lycopersicum esculentum (tomato) growth dynamics. ZnO-NS prepared via green chemistry possesses highly homogenous crystalline structures well-characterised through ultraviolet and visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscope...
June 2018: IET Nanobiotechnology
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