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https://www.readbyqxmd.com/read/29785739/in-vitro-formation-of-the-merkel-cell-neurite-complex-in-embryonic-mouse-whiskers-using-organotypic-co-cultures
#1
Kentaro Ishida, Tetsuichiro Saito, Toshiyuki Mitsui
A Merkel cell-neurite complex is a touch receptor composed of specialized epithelial cells named Merkel cells and peripheral sensory nerves in the skin. Merkel cells are found in touch-sensitive skin components including whisker follicles. The nerve fibers that innervate Merkel cells of a whisker follicle extend from the maxillary branch of the trigeminal ganglion. Whiskers as a sensory organ attribute to the complicated architecture of the Merkel cell-neurite complex, and therefore it is intriguing how the structure is formed...
May 22, 2018: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/29785565/supplementation-strategies-for-nellore-female-calves-in-creep-feeding-to-improve-the-performance-nutritional-and-metabolic-responses
#2
Daniel Mageste de Almeida, Marcos Inácio Marcondes, Luciana Navajas Rennó, Leandro Soares Martins, David Esteban Contreras Marquez, Faider Castaño Villadiego, Felipe Velez Saldarriaga, Román Maza Ortega, Deilen Paff Sotelo Moreno, Sidnei Antônio Lopes, Manuela Acevedo Cardozo, Mário Fonseca Paulino
The objective of this study was to evaluate the effects of supplementation strategy on performance, nutritional and metabolic characteristics of Nellore suckling female calves, on grazing Urochloa decumbens during the rainy-dry transition period. Forty-four Nellore female calves, averaging 147.6 ± 1.34 kg (4 months), were used. A single supplement 20% of crude protein of dry matter was provided, consisting of daily supplement at 0.0, 0.2, 0.4, or 0.6% of body weight. A positive linear effect (P < 0...
May 21, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29783766/finite-element-analysis-of-electrospun-nanofibrous-mats-under-biaxial-tension
#3
Yunlei Yin, Jie Xiong
Due to the non-uniform material properties of electrospun nanofibrous mats and the non-linear characteristics of single fibers, establishing a numerical model that can fully explain these features and correctly describe their properties is difficult. Based on the microstructure of electrospun nanofibrous mats, two macroscopic continuum finite element (FE) models with a uniform or oriented nanofiber distribution were established to describe the mechanical behavior of nanofibrous mats under biaxial tension. The FE models were verified by biaxial tension experiments on silk fibroin/polycaprolactone nanofibrous mats...
May 19, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29781944/early-comparative-outcomes-of-carbon-fiber-reinforced-polymer-plate-in-the-fixation-of-distal-femur-fractures
#4
Phillip M Mitchell, Adam K Lee, Cory A Collinge, Bruce H Ziran, Kate G Hartley, A Alex Jahangir
OBJECTIVE: To evaluate the early clinical results of distal femur fractures treated with carbon fiber reinforced - polyetheretherketone (CFR-PEEK) plates compared to stainless steel (SS) lateral locking plates. DESIGN: Retrospective comparative cohort study SETTING:: ACS Level I trauma center. PATIENTS/PARTICIPANTS: Twenty-two patients (11 SS, 11 CFR-PEEK) with closed distal femur fractures treated by a single surgeon over a 6-year period...
May 16, 2018: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29781580/neuromuscular-electrical-stimulation-compared-to-volitional-exercise-in-improving-muscle-function-in-rheumatoid-arthritis-a-randomized-pilot-study
#5
Sara R Piva, Samannaaz S Khoja, Frederico G S Toledo, Mary Chester Wasko, G Kelley Fitzgerald, Bret H Goodpaster, Clair N Smith, Anthony Delitto
OBJECTIVES: To compare the feasibility and effectiveness of Neuromuscular Electrical Stimulation (NMES) with high-intensity volitional resistance training in improving muscle structure and function, and physical function in patients with rheumatoid arthritis (RA). We also described pre- and post-intervention values of myocyte characteristics. METHODS: Two-group single-blinded pilot randomized study in adults with RA. Participants were assigned to 36 sessions over 16 weeks of NMES (n=31) or volitional training (n=28)...
May 21, 2018: Arthritis Care & Research
https://www.readbyqxmd.com/read/29781171/fiber-based-fluorescence-lifetime-imaging-of-recellularization-processes-on-vascular-tissue-constructs
#6
Alba Alfonso-Garcia, Jeny Shklover, Benjamin E Sherlock, Alyssa Panitch, Leigh G Griffiths, Laura Marcu
New techniques able to monitor the maturation of tissue engineered constructs over time are needed for a more efficient control of developmental parameters. Here, a label-free fluorescence lifetime imaging (FLIm) approach implemented through a single fiber-optic interface is reported for non-destructive in situ assessment of vascular biomaterials. Recellularization processes of antigen removed bovine pericardium scaffolds with endothelial cells and mesenchymal stem cells were evaluated on the serous and the fibrous sides of the scaffolds, two distinct extracellular matrix niches, over the course of a 7 day culture period...
May 21, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29780879/altered-cerebellar-short-term-plasticity-but-no-change-in-postsynaptic-ampa-type-glutamate-receptors-in-a-mouse-model-of-juvenile-batten-disease
#7
Dorota Studniarczyk, Elizabeth L Needham, Hannah M Mitchison, Mark Farrant, Stuart G Cull-Candy
Juvenile Batten disease is the most common progressive neurodegenerative disorder of childhood. It is associated with mutations in the CLN3 gene, causing loss of function of CLN3 protein and degeneration of cerebellar and retinal neurons. It has been proposed that changes in granule cell AMPA-type glutamate receptors (AMPARs) contribute to the cerebellar dysfunction. In this study, we compared AMPAR properties and synaptic transmission in cerebellar granule cells from wild-type and Cln3 knock-out mice. In Cln3 Δ ex1-6 cells, the amplitude of AMPA-evoked whole-cell currents was unchanged...
March 2018: ENeuro
https://www.readbyqxmd.com/read/29780501/a-bi-terminal-protein-ligation-strategy-to-probe-chromatin-structure-during-dna-damage
#8
Sinan Kilic, Iuliia Boichenko, Carolin C Lechner, Beat Fierz
The cellular response to DNA damage results in a signaling cascade that primes chromatin for repair. Combinatorial post-translational modifications (PTMs) play an important role in this process by altering the physical properties of chromatin and recruiting downstream factors. One key signal integrator is the histone variant H2A.X, which is phosphorylated at a C-terminal serine (S139ph), and ubiquitylated within its N-terminal tail at lysines 13 and 15 (K13/15ub). How these PTMs directly impact chromatin structure and thereby facilitate DNA repair is not well understood...
April 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780222/role-of-concentric-needle-single-fiber-electromyography-in-detection-of-subclinical-motor-involvement-in-carpal-tunnel-syndrome
#9
Aliaa A Tawfeek, Amani M Nawito, Radwa M Azmy, Amr Hassan, Lamia M Afifi, Saly H Elkholy
Background: Conventional motor nerve conduction studies are usually normal in early and mild carpal tunnel syndrome (CTS). Single-fiber electromyography (SFEMG) measures the mean consecutive difference (MCD) as an expression of the variability in impulse transmission over the motor endplates and along the nerve fibers distally to the last branching point and along the muscle fibers.Application of concentric needle SFEMG in a group of CTS patients who showed pure sensory abnormalities in nerve conduction studies to examine for subclinical motor involvement...
2018: The Egyptian journal of neurology, psychiatry and neurosurgery
https://www.readbyqxmd.com/read/29777431/modified-anterior-palatoplasty-and-double-suspension-sutures-with-or-without-tonsillectomy-in-selected-patients-with-obstructive-sleep-apnea-a-preliminary-report
#10
Sherif M Askar, Mohammad W El-Anwar, Ali Awad
BACKGROUND: Retropalatal region might be considered the most common site of obstruction in patients with snoring and OSA. We aimed at presentation of two combined techniques: modified anterior palatoplasty (MAP) and bilateral double suspension suture (DSS). METHODS: This prospective study was conducted on OSA patients with retro-palatal collapse only that was confirmed by nasoendoscopy during Muller's maneuver and drug induced sleep endoscopy. MAP and DSS were performed for all patients...
May 18, 2018: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/29775854/a-wavelength-modulated-localized-surface-plasmon-resonance-lspr-optical-fiber-sensor-for-sensitive-detection-of-mercury-ii-ion-by-gold-nanoparticles-dna-conjugates
#11
Shuo Jia, Chao Bian, Jizhou Sun, Jianhua Tong, Shanhong Xia
The study presented herein investigated an easy preparation, high performance, wavelength-modulated LSPR optical fiber chemosensor coated by gold nanospheres(AuNS) for Hg2+ detection based on thymine-Hg2+ -thymine base pair mismatches and the coupled plasmonic resonance effect.Utilizing electrostatic self-assembly method, the high density and dispersivity monolayer AuNS coated LSPR fiber sensor had the near field refractive index sensitivity up to 2016 nm/RIU. The single-strand probe DNA served as a binding element for free AuNS labelled-target DNA conjugates was attached to the monolayer AuNS by Au-S bond...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29775410/apelin-and-apj-orchestrate-complex-tissue-specific-control-of-cardiomyocyte-hypertrophy-and-contractility-in-the-hypertrophy-heart-failure-transition
#12
Victoria Nicole Parikh, Jing Liu, Ching Shang, Christopher Woods, Alex Chia Yu Chang, Mingming Zhao, David N Charo, Zachary Grunwald, Yong Huang, Kinya Seo, Philip S Tsao, Daniel Bernstein, Pilar Ruiz-Lozano, Thomas Quertermous, Euan A Ashley
The G protein coupled receptor APJ is a promising therapeutic target for heart failure. Constitutive deletion of APJ in the mouse is protective against the hypertrophy-heart failure transition via elimination of ligand-independent, β-arrestin dependent stretch transduction. However, the cellular origin of this stretch transduction and the details of its interaction with apelin signaling remain unknown. We generated mice with conditional elimination of APJ in the endothelium (APJendo-/- ) and myocardium (APJmyo-/- )...
May 18, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29774991/aggregate-mesenchymal-stem-cell-delivery-ameliorates-the-regenerative-niche-for-muscle-repair
#13
Marissa A Ruehle, Hazel Y Stevens, Aaron M Beedle, Robert E Guldberg, Jarrod A Call
Duchenne muscular dystrophy (DMD) is a severe muscle wasting disease due to the absence of the dystrophin protein from the muscle cell membrane which renders the muscle susceptible to continuous damage. In DMD patients, muscle weakness, together with cycles of degeneration/regeneration and replacement with non-contractile tissue, limit mobility and lifespan. Since the loss of dystrophin result in loss of polarity and a reduction in the number of self-renewing satellite cells, it is postulated that these patients could achieve an improved quality of life if delivered cells could restore satellite cell function...
May 18, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29773387/levan-based-fibrous-scaffolds-electrospun-via-co-axial-and-single-needle-techniques-for-tissue-engineering-applications
#14
Gülben Avsar, Deniz Agirbasli, Mehmet Ali Agirbasli, Oguzhan Gunduz, Ebru Toksoy Oner
This represents the first systematic study where levan polysaccharide was used to fabricate fibrous matrices by co-axial and single-needle electrospinning techniques. For this, hydrolyzed (hHL) and sulfated hydrolyzed (ShHL) Halomonas levan were chemically synthesized and used together with polycaprolactone (PCL) and polyethyleneoxide (PEO) for the spinning process. In co-axially spun matrices, ultimate tensile strength (UTS) were found to increase with increasing ShHL concentration and elongation at break of PCL + ShHL matrices increased up to ten-fold when compared to PCL matrices...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29772841/the-effect-of-human-mesenchymal-stem-cells-derived-from-wharton-s-jelly-in-spinal-cord-injury-treatment-is-dose-dependent-and-can-be-facilitated-by-repeated-application
#15
Petr Krupa, Irena Vackova, Jiri Ruzicka, Kristyna Zaviskova, Jana Dubisova, Zuzana Koci, Karolina Turnovcova, Lucia Machova Urdzikova, Sarka Kubinova, Svatopluk Rehak, Pavla Jendelova
Human mesenchymal stem cells derived from Wharton's jelly (WJ-MSCs) were used for the treatment of the ischemic-compression model of spinal cord injury in rats. To assess the effectivity of the treatment, different dosages (0.5 or 1.5 million cells) and repeated applications were compared. Cells or saline were applied intrathecally by lumbar puncture for one week only, or in three consecutive weeks after injury. Rats were assessed for locomotor skills (BBB, rotarod, flat beam) for 9 weeks. Spinal cord tissue was morphometrically analyzed for axonal sprouting, sparing of gray and white matter and astrogliosis...
May 17, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29770800/current-and-future-circulating-biomarkers-for-cardiac-amyloidosis
#16
REVIEW
Marco Luciani, Luca Troncone, Federica Del Monte
Cardiac amyloidosis (CA) comprises a heterogeneous group of medical conditions affecting the myocardium. It presents with proteinaceous infiltration with variable degrees of severity, prevalence and evolution. Despite this heterogeneity, erroneous protein folding is the common pathophysiologic process, yielding the formation of a single misfolded protein (monomer) that progressively evolves and ultimately forms amyloid fibers. Additionally, by seeding out from the organs of origin, intermediates called oligomers metastasize and restart the process...
May 17, 2018: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/29762606/intensity-modulated-directional-torsion-sensor-based-on-in-line-optical-fiber-mach-zehnder-interferometer
#17
Qinjiang Fu, Jingdong Zhang, Chuancan Liang, Iroegbu Paul Ikechukwu, Guolu Yin, Lei Lu, Yifan Shao, Liu Liu, Dongxiao Liu, Tao Zhu
In this Letter, we demonstrated an intensity-modulated directional torsion sensor based on an in-line Mach-Zehnder interferometer in single-mode fiber. A non-circular symmetric perturbation is created to excite non-circular symmetric cladding mode and then interference with the core mode at the second perturbation. An initial rotation angle is designed between two perturbations for the purpose of discriminating the torsion direction. Both experimental and theoretical results enforce that the spectral peak/dip turns to be the dip/peak when the fiber is twisted from the counter-clockwise to the clockwise direction...
May 15, 2018: Optics Letters
https://www.readbyqxmd.com/read/29762601/demonstration-of-a-highly-stable-10-ghz-optical-frequency-comb-with-low-timing-jitter-from-a-scowa-based-harmonically-mode-locked-nested-cavity-laser
#18
Kristina Bagnell, Anthony Klee, Peter J Delfyett, Jason J Plant, Paul W Juodawlkis
An optical frequency comb with mode spacing of 10 GHz operating in the c-band is produced from a harmonically mode-locked laser using a slab-coupled optical waveguide amplifier device with a fiber-coupled external cavity. An intracavity Fabry-Perot etalon serves as a high finesse optical filter for supermode suppression and as the reference for cavity length stabilization using a multi-combline Pound-Drever-Hall setup. The Allan deviation of a single optical combline near 193.4 THz is measured via a heterodyne beat with a cavity stabilized cw laser and reaches a minimum fractional frequency deviation of 3×10-13 at τ=30  ms...
May 15, 2018: Optics Letters
https://www.readbyqxmd.com/read/29762584/wideband-tunable-optoelectronic-oscillator-based-on-a-microwave-photonic-filter-with-an-ultra-narrow-passband
#19
Haitao Tang, Yuan Yu, Ziwei Wang, Lu Xu, Xinliang Zhang
A novel wideband tunable optoelectronic oscillator based on a microwave photonic filter (MPF) with an ultra-narrow passband is proposed and experimentally demonstrated. The single-passband MPF is realized by cascading an MPF based on stimulated Brillouin scattering and an infinite impulse response (IIR) MPF based on an active fiber recirculating delay loop. The measured full width at half-maximum bandwidth of the cascaded MPFs is 150 kHz. To the best of my knowledge, this is the first time realizing such a narrow passband in single-passband MPF...
May 15, 2018: Optics Letters
https://www.readbyqxmd.com/read/29762569/transition-of-fabry-perot-and-antiresonant-mechanisms-via-a-smf-capillary-smf-structure
#20
Xiaobei Zhang, Haiyang Pan, Huawen Bai, Ming Yan, Jiawei Wang, Chuanlu Deng, Tingyun Wang
In this Letter, we investigate the transition of the well-known Fabry-Perot (FP) and antiresonant (AR) mechanisms via a single-mode fiber (SMF)-capillary-SMF structure. The critical length for this transition is analytically found as a linear relation with the capillary inner diameter based on the ray optic method, which shows the agreement with both numerical simulations and experiments. Evolutions of the transmission and reflection spectra verify that FP and AR mechanisms are closely related to the critical length...
May 15, 2018: Optics Letters
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