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High performance liquid chromatography

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https://www.readbyqxmd.com/read/28528271/exploring-binding-characteristics-and-the-related-competition-of-different-protein-bound-uremic-toxins
#1
Olivier Deltombe, Henriette de Loor, Griet Glorieux, Annemieke Dhondt, Wim Van Biesen, Björn Meijers, Sunny Eloot
Little is known about potential differences in binding characteristics of protein-bound uremic toxins (PBUTs) in patients with chronic kidney disease (CKD) versus healthy controls. The question arises whether eventual differences are attributed to (i) the elevated levels of competing uremic toxins, and/or (ii) post-translational modifications of albumin. We evaluated the binding characteristics of hippuric acid (HA), indole-3-acetic acid (IAA), indoxyl sulfate (IS), and p-cresylsulfate (pCS) by deriving a binding curve in three distinct conditions: (i) serum from healthy controls (healthy serum), (ii) blank serum from hemodialysis patients (blank HD serum; i...
May 17, 2017: Biochimie
https://www.readbyqxmd.com/read/28528229/sensitive-uhplc-ms-ms-quantitation-and-pharmacokinetic-comparisons-of-multiple-alkaloids-from-fuzi-beimu-and-single-herb-aqueous-extracts-following-oral-delivery-in-rats
#2
Yanyan Xu, Yamei Li, Pengjie Zhang, Bin Yang, Huanyu Wu, Xuejun Guo, Yubo Li, Yanjun Zhang
Aconiti Lateralis Radix Praeparata- Fritillariae Thunbergii bulbus, namely Fuzi- Beimu in Chinese, is a classic herb pair whose combined administration was prohibited according to the rule of "Eighteen antagonisms". However, incompatibility of Fuzi and Beimu has become controversial because of the application supported by many recorded ancient prescriptions and increasing modern researches and clinical practice. The present study aimed to investigate the pharmacokinetic differences of multiple alkaloids from Fuzi- Beimu and the single herb aqueous extracts following oral delivery in rats...
May 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28528106/characterization-of-white-tea-metabolome-comparison-against-green-and-black-tea-by-a-nontargeted-metabolomics-approach
#3
Weidong Dai, Dongchao Xie, Meiling Lu, Pengliang Li, Haipeng Lv, Chen Yang, Qunhua Peng, Yin Zhu, Li Guo, Yue Zhang, Junfeng Tan, Zhi Lin
White tea is considered the least processed form of tea and is reported to have a series of potent bioactivities, such as antioxidant, anti-inflammatory, anti-mutagenic, and anti-cancer activities. However, the chemical composition of white tea and the dynamic changes of the metabolites during the manufacturing process are far from clear. In this study, we applied a nontargeted metabolomics approach based on ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) to comprehensively profile the characteristic metabolites of white tea...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28527994/purification-and-characterization-of-a-protease-resistant-phytase-of-aspergillus-oryzae-sbs50-whose-properties-make-it-exceptionally-useful-as-a-feed-supplement
#4
Sapna, Bijender Singh
An extracellular phytase of Aspergillus oryzae SBS50 was purified to homogeneity using ammonium sulphate precipitation, ion-exchange and gel filtration chromatography. Purified phytase has a monomeric molecular mass of ∼80kDa exhibiting its optimal activity at pH 5.0 and 50°C with a T 1/2 of 300min at 50°C. Phytase of A. oryzae displayed broad substrate specificity with Vmax and Km values of 58.82μmol/ml/min and 1.14mM, respectively, for calcium phytate. Purity and homogeneity of the phytase was confirmed by high performance liquid chromatography and MALDI-TOF analysis revealed the identification of a peptide showing homology with acid phosphatase of Aspergillus oryzae RIB40...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28527826/metabolic-pathway-analysis-of-the-xylose-metabolizing-yeast-protoplast-fusant-zlyrhz7
#5
Jingping Ge, Renpeng Du, Gang Song, Yuhuan Zhang, Wenxiang Ping
Xylose is the second major fermentable sugar present in hard woods and herbs (after d-glucose). Therefore, efficient conversion of xylose to ethanol is essential for the commercialization of lignocellulosic ethanol, which may provide an ideal alternative to fossil fuels in the future. ZLYRHZ7 is a fusant produced by protoplast fusion between two different yeast species, Saccharomyces cerevisiae W5 and Candida shehatae 20335, which is able to utilize xylose to produce ethanol. To improve ethanol production and to quantitatively analyze metabolic pathway in ZLYRHZ7, we used high performance liquid chromatography (HPLC) to assess the utilization rates of xylose, xylitol, and xylulose, and to measure ethanol yields using xylose, xylitol, and xylulose as sole carbon sources...
May 17, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28527785/the-effectiveness-of-a-fixed-dose-combination-pour-on-formulation-of-1-25-fipronil-and-2-5-fluazuron-against-economically-important-ectoparasites-and-associated-pharmacokinetics-in-cattle
#6
Welber Daniel Zanetti Lopes, Rafael Marin Chiummo, Luis Fernando Vettorato, Daniel de Castro Rodrigues, Rafael Bazaglia Sonada
The present work consisted of eight studies to evaluate the ectoparasiticidal spectrum and determine the pharmacokinetic parameters of a pour-on combination of fipronil 1.25mg/kg+fluazuron 2.5mg/kg for cattle against Rhipicephalus microplus, Haematobia irritans and the larvae of Dermatobia hominis and Cochliomyia hominivorax. The analysis fipronil and fluazuron were performed by liquid chromatography using a mass detector for the detection and quantification of analytes (LC-MS/MS). Additionally, in two of these studies, the animals were artificially infested with R...
May 17, 2017: Parasitology International
https://www.readbyqxmd.com/read/28527719/involvement-of-serotonin-in-the-ventral-tegmental-area-in-thermoregulation-of-freely-moving-rats
#7
Takayuki Ishiwata, Hiroshi Hasegawa, Benjamin N Greenwood
We have recently reported that the serotonin (5-HT) projections from the midbrain's raphe nuclei that contains 5-HT cell bodies may play a role both in heat production and in heat loss. The purpose of the present study was to clarify the involvement of 5-HT in the ventral tegmental area (VTA), where 5-HT is suggested to participate in thermoregulation, using the combined methods of telemetry, microdialysis, and high performance liquid chromatography, with a special emphasis on regulation of the body temperature (Tb) in freely moving rats...
May 17, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28527517/holistic-evaluation-of-san-huang-tablets-using-a-combination-of-multi-wavelength-quantitative-fingerprinting-and-radical-scavenging-assays
#8
Yan Wang, Guo-Xiang Sun, Yang Jin, Xiu-Man Xie, Ying-Chun Liu, Di-Di Ma, Jing Zhang, Jia-Yue Gao, Yan-Fei Li
The present study was designed to establish a multi-wavelength quantitative fingerprinting method for San-Huang Tablets (SHT), a widely used and commercially available herbal preparation, where high performance liquid chromatography (HPLC) with a diode array detector (DAD) was employed to obtain the fingerprint profiles. A simple linear quantitative fingerprint method (SLQFM) coupled with multi-ingredient simultaneous determination was developed to evaluate the quality consistency of the tested samples qualitatively and quantitatively...
April 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28527414/structural-characterization-and-discrimination-of-the-paris-polyphylla-var-yunnanensis-and-paris-vietnamensis-based-on-metabolite-profiling-analysis
#9
Li-Ping Kang, Yuan-Yuan Huang, Zhi-Lai Zhan, Da-Hui Liu, Hua-Sheng Peng, Tie-Gui Nan, Yuan Zhang, Qing-Xiu Hao, Jin-Fu Tang, Shou-Dong Zhu, Guang Yang, Lan-Ping Guo, Min Chen, Lu-Qi Huang
This study aimed to distinguish the rhizomes of Paris polyphylla var. yunnanensis (Franch) Hand Mazz (PPY) and Paris veitnamensis (Takht.) H. Li (PV) using metabolomics-based ultra high-performance liquid chromatography coupled with quadrupole time-of-fligh mass spectrometry (UHPLC/Q-TOF MS). First, the UHPLC/Q-TOF MS approach was optimized for metabolite profiling. Then, the MS data were processed using UNIFI™ combined with an in-house library to automatically characterize the metabolites. Based on the exact mass information, the fragmentation characteristics, and the retention time of compounds, and the fragmentation mechanism and retention behavior of steroidal glycosides in the references, the structures identified by UNIFI were further verified...
May 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28527322/arsenic-biotransformation-by-a-cyanobacterium-nostoc-sp-pcc-7120
#10
Xi-Mei Xue, Yu Yan, Chan Xiong, Georg Raber, Kevin Francesconi, Ting Pan, Jun Ye, Yong-Guan Zhu
Nostoc sp. PCC 7120 (Nostoc), a typical filamentous cyanobacterium ubiquitous in aquatic system, is recognized as a model organism to study prokaryotic cell differentiation and nitrogen fixation. In this study, Nostoc cells incubated with arsenite (As(III)) for two weeks were extracted with dichloromethane/methanol (DCM/MeOH) and the extract was partitioned between water and DCM. Arsenic species in aqueous and DCM layers were determined using high performance liquid chromatography - inductively coupled plasma mass spectrometer/electrospray tandem mass spectrometry (HPLC-ICPMS/ESIMSMS)...
May 17, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28527015/a-genome-wide-association-study-identifies-the-genomic-region-associated-with-shell-color-in-yesso-scallop-patinopecten-yessoensis
#11
Liang Zhao, Yangping Li, Yajuan Li, Jiachen Yu, Huan Liao, Shuyue Wang, Jia Lv, Jun Liang, Xiaoting Huang, Zhenmin Bao
The shell color polymorphism widely exists in economic shellfish, which not only results in a better visual perception but also shows great value as an economic trait for breeding. Small numbers of reddish-orange shell Yesso scallops, Patinopecten yessoensis, were found in cultured populations compared to the brown majority. In this study, a genome-wide association study was conducted to understand the genetic basis of shell color. Sixty-six 2b-RAD libraries with equal numbers of reddish-orange and brown shell individuals were constructed and sequenced using the Illumina HiSeq 2000 platform...
May 19, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28526446/thymoquinone-exerts-neuroprotective-effect-in-animal-model-of-parkinson-s-disease
#12
Seyedeh Shohreh Ebrahimi, Shahrbanoo Oryan, Esmael Izadpanah, Kambiz Hassanzadeh
Oxidative stress plays an important role in both the initiation and progression of Parkinson's disease (PD). Rotenone, an environmental toxin, induces oxidative stress and impact mitochondrial dynamics, including fission and fusion. Thymoquinone (TQ) has been reported to have antioxidant and anti-inflammatory characteristics in vitro and in vivo. TQ scavenges free radicals so prevents cell damage against oxidative agents. To evaluate the efficacy of TQ in the management of PD, male Wistar rats (8-10 months) received rotenone...
May 16, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28525869/hepatic-microsomal-metabolism-of-bde-47-and-bde-99-by-lesser-snow-geese-and-japanese-quail
#13
Lisa K Krieger, András Szeitz, Stelvio M Bandiera
In the present study, we investigated the oxidative biotransformation of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and 2,2',4,4',5-pentabromodiphenyl ether (BDE-99) by liver microsomes from wild lesser snow geese (Chen caerulescens caerulescens) and domesticated Japanese quail (Coturnix japonica). Formation of hydroxy-metabolites was analyzed using an ultra-high performance liquid chromatography-tandem mass spectrometry-based method. Incubation of BDE-47 with avian liver microsomes produced sixteen hydroxy-metabolites, eight of which were identified using authentic standards...
May 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/28525791/determination-of-thiopurine-s-methyltransferase-activity-by-hydrophilic-interaction-liquid-chromatography-hyphenated-with-mass-spectrometry
#14
Daniel Pecher, Svetlana Dokupilová, Zuzana Zelinková, Maikel Peppelenbosch, Veronika Mikušová, Peter Mikuš
Thiopurine S-methyltransferase (TPMT) plays an important role in the metabolism of thiopurines used in the therapy of inflammatory bowel diseases (IBD). In this work a new progressive method for the determination of TPMT activity in red blood cells lysates was developed. Analysis was carried out by means of hydrophilic interaction liquid chromatography (HILIC) hyphenated with mass spectrometry (MS). In comparison with reversed-phase high-performance liquid chromatography (RP-HPLC), that has been typically applied in determination of TPMT activity, the HILIC significantly improved the analytical signal provided by MS, shortened analysis time, and improved chromatographic resolution...
May 10, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28524256/quantification-of-l-carvone-in-toothpastes-available-on-the-swedish-market
#15
Liv Kroona, Gunnar Warfvinge, Marléne Isaksson, Camilla Ahlgren, Jakob Dahlin, Östen Sörensen, Magnus Bruze
BACKGROUND: Toothpastes have widespread use in the population, and contain flavours used to give a pleasant and often minty aroma. Flavours are prevalent allergens in toothpastes, and adverse reactions often present as perioral dermatitis or stomatitis. l-Carvone, a mint flavour found in spearmint oil, is one of these allergens. There are few studies on contact allergy to l-carvone, and some of them have indicated a positive relationship with oral lichenoid lesions. OBJECTIVES: To qualitatively and quantitatively investigate the presence of l-carvone in commercially available toothpastes on the Swedish market...
May 19, 2017: Contact Dermatitis
https://www.readbyqxmd.com/read/28521276/an-atmospheric-pressure-ionization-source-using-a-high-voltage-target-compared-to-electrospray-ionization-for-the-lc-ms-analysis-of-pharmaceutical-compounds
#16
Arnaud Lubin, Ronald De Vries, Deirdre Cabooter, Patrick Augustijns, Filip Cuyckens
The type and design of an ionization source can have a significant influence on the performances of a bioanalytical method. It is, therefore, of high interest to evaluate the performances of newly introduced sources to highlight their benefits and limitations in comparison to other well established sources. In this paper, liquid chromatography - mass spectrometry (LC/MS) performances of a new atmospheric pressure ionization (API) source, commercialized as UniSpray, is evaluated. The dynamic range of 24 pharmaceutical and biological compounds is compared between the new API source and electrospray ionization (ESI) for 3 different mobile phase conditions...
May 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28521163/application-for-oxytetracycline-wastewater-pretreatment-by-fenton-iron-mud-based-cathodic-anodic-electrolysis-ceramic-granular-fillers
#17
Feilong Zhang, Qinyan Yue, Yuan Gao, Baoyu Gao, Xing Xu, Zhongfei Ren, Yang Jin
In this study, Fenton iron mud applied as main raw material of cathodic-anodic-electrolysis ceramic granular fillers (ICMF) in a continuous reactor, which were used to pretreat oxytetracycline (OTC) wastewater. The ICMF was characterized by Scanning Electron Microscope and Energy Dispersive Spectrometer analysis. The effects of pH value, hydraulic retention time, OTC concentrations and aeration on removal efficiency of total organic carbon (TOC) and OTC were studied. The degradation byproducts of OTC were analyzed by UV-2450, High Performance Liquid Chromatography and Liquid Chromatography-mass Spectrometry...
May 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28516279/a-new-carbon-based-magnetic-material-for-the-dispersive-solid-phase-extraction-of-uv-filters-from-water-samples-before-liquid-chromatography-tandem-mass-spectrometry-analysis
#18
Susy Piovesana, Anna Laura Capriotti, Chiara Cavaliere, Giorgia La Barbera, Roberto Samperi, Riccardo Zenezini Chiozzi, Aldo Laganà
Magnetic solid-phase extraction is one of the most promising new extraction methods for liquid samples before ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. Several types of materials, including carbonaceous ones, have been prepared for this purpose. In this paper, for the first time, the preparation, characterization, and sorption capability of Fe3O4-graphitized carbon black (mGCB) composite toward some compounds of environmental interest were investigated. The synthesized mGCB consisted of micrometric GCB particles with 55 m(2) g(-1) surface area bearing some carbonyl and hydroxyl functionalities and the surface partially decorated by Fe3O4 microparticles...
May 18, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28516097/asymmetric-dimethylarginine-in-patients-with-ascending-aortic-aneurysms
#19
Natalia D Gavriliuk, Tatiana A Druzhkova, Olga B Irtyuga, Alexandr A Zhloba, Tatiana F Subbotina, Vladimir E Uspenskiy, Nina P Alexeyeva, Olga M Moiseeva
BACKGROUND: Ascending thoracic aortic aneurysm (aTAA) is a heterogeneous group of disorders that involve impaired endothelial function. The nitric oxide (NO) synthase inhibitor asymmetric dimethylarginine (ADMA) serves as an endothelial dysfunction marker. Thus, we investigated ADMA levels in patients with aTAA. METHODS: Eighty-six patients with aTAA and 18 healthy individuals were enrolled. All patients underwent echocardiography. Plasma ADMA levels were measured using high-performance liquid chromatography...
December 2016: Aorta (Stamford, Conn.)
https://www.readbyqxmd.com/read/28516056/simultaneous-micronization-and-purification-of-bioactive-fraction-by-supercritical-antisolvent-technology
#20
Stevanus Hiendrawan, Bambang Veriansyah, Edward Widjojokusumo, Raymond R Tjandrawinata
Simultaneous micronization and purification of DLBS3233 bioactive fraction, a combination of two Indonesian herbals Lagerstroemia speciosa and Cinnamomum burmannii has been successfully performed via supercritical anti-solvent (SAS) technology. The objective of the present study was to investigate the effectiveness of SAS technology to micronize and reduce coumarin content of DLBS3233. The effects of four SAS process parameters, i.e. pressure, temperature, concentration and solution flow rate on particle formation were investigated...
April 2017: Journal of Advanced Pharmaceutical Technology & Research
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