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High performance liquid chromatography

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https://www.readbyqxmd.com/read/27923200/a-quantitative-lc-ms-ms-method-for-simultaneous-determination-of-cocaine-and-its-metabolites-in-whole-blood
#1
Xiabin Chen, Xirong Zheng, Kai Ding, Ziyuan Zhou, Chang-Guo Zhan, Fang Zheng
As new metabolic pathways of cocaine were recently identified, a high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously determine cocaine and nine cocaine-related metabolites in whole blood samples. One-step solid phase extraction was used to extract all of the ten compounds and corresponding internal standards from blood samples. All compounds and internal standards extracted were separated on an Atlantis T3 (100Å, 3μm, 2.1mm×150mm I.D) column and detected in positive ion and high sensitivity mode with multiple reaction monitoring...
November 11, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27921399/antioxidant-and-antidiabetic-properties-of-tartary-buckwheat-rice-flavonoids-after-in-vitro-digestion
#2
Tao Bao, Ye Wang, Yu-Ting Li, Vemana Gowd, Xin-He Niu, Hai-Ying Yang, Li-Shui Chen, Wei Chen, Chong-de Sun
Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzymes, thereby decreasing gastrointestinal glucose production. Plant-derived natural antioxidant molecules are considered a therapeutic tool in the treatment of oxidative stress and diabetes. The objective of this study was to identify tartary buckwheat rice flavonoids and evaluate the effect of in vitro digestion on their antioxidant and antidiabetic properties...
2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27921079/data-file-of-a-deep-proteome-analysis-of-the-prefrontal-cortex-in-aged-mice-with-progranulin-deficiency-or-neuronal-overexpression-of-progranulin
#3
Juliana Heidler, Stefanie Hardt, Ilka Wittig, Irmgard Tegeder
Progranulin deficiency is associated with neurodegeneration in humans and in mice. The mechanisms likely involve progranulin-promoted removal of protein waste via autophagy. We performed a deep proteomic screen of the pre-frontal cortex in aged (13-15 months) female progranulin-deficient mice (GRN(-/-)) and mice with inducible neuron-specific overexpression of progranulin (SLICK-GRN-OE) versus the respective control mice. Proteins were extracted and analyzed per liquid chromatography/mass spectrometry (LC/MS) on a Thermo Scientific™ Q Exactive Plus equipped with an ultra-high performance liquid chromatography unit and a Nanospray Flex Ion-Source...
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27920527/pharmacokinetics-and-safety-of-dts-108-a-human-oligopeptide-bound-to-sn-38-with-an-esterase-sensitive-cross-linker-in-patients-with-advanced-malignancies-a-phase-i-study
#4
Romain Coriat, Sandrine J Faivre, Olivier Mir, Chantal Dreyer, Stanislas Ropert, Mohammed Bouattour, Robert Desjardins, François Goldwasser, Eric Raymond
BACKGROUND: DTS-108 is a hydrosoluble prodrug, where the SN-38 moiety is covalently linked to a 20-amino acid vector peptide by a specific esterase-sensitive cross-linker, releasing 7-ethyl-10-hydroxycampthotecin (SN-38) by esterase bond cleavage. METHODS: The pharmacokinetics of DTS-108, adverse events graded according to NCI-CTCv3.1, dose-limiting toxicities at cycle 1, the maximum tolerated dose (MTD), and the recommended Phase II dose (RP2D) of intravenous DTS-108 (1-2 hours) every 2 weeks were evaluated in a first-in-human Phase I study in patients with advanced/metastatic carcinomas, according to an accelerated dose escalation design...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27919876/stability-of-tacrolimus-injection-diluted-in-0-9-sodium-chloride-injection-and-stored-in-excel-bags
#5
Alan L Myers, Yanping Zhang, Jitesh D Kawedia, Brandon R Shank, Melissa A Deaver, Mark A Kramer
PURPOSE: The chemical stability and physical compatibility of tacrolimus i.v. infusion solutions prepared in Excel bags and stored at 23 or 4 °C for up to nine days were studied. METHODS: Tacrolimus admixtures (2, 4, and 8 μg/mL) were prepared in Excel bags using 0.9% sodium chloride injection and stored at 23 °C without protection from light or at 4 °C in the dark. Test samples were withdrawn from triplicate bag solutions immediately after preparation and at predetermined time intervals (1, 3, 5, 7, and 9 days)...
December 15, 2016: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/27919258/preparation-and-structural-determination-of-four-metabolites-of-senkyunolide-i-in-rats-using-ultra-performance-liquid-chromatography-quadrupole-time-of-flight-tandem-mass-and-nuclear-magnetic-resonance-spectra
#6
Qiang Ma, Cong Ma, Fei Wu, Yao-Kun Xiong, Yi Feng, Shuang Liang
BACKGROUND: Senkyunolide I (SEI) is one of the most important bioactive phthalides of Ligusticum chuanxiong Hort. (Umbelliferae), a Traditional Chinese Medicine. Our previous studies suggested that it might be developed as a potential treatment for migraine. METHODS: In this paper, we aimed to isolate and characterize the main metabolites of SEI after gavage feeding in rats. Their structures were identified precisely on the basis of nuclear magnetic resonance (NMR) spectroscopy and UPLC/Q-TOF-MS spectrometry...
December 5, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27918445/docetaxel-loaded-self-assembly-stearic-acid-modified-bletilla-striata-polysaccharide-micelles-and-their-anticancer-effect-preparation-characterization-cellular-uptake-and-in-vitro-evaluation
#7
Qingxiang Guan, Dandan Sun, Guangyuan Zhang, Cheng Sun, Miao Wang, Danyang Ji, Wei Yang
Poorly soluble drugs have low bioavailability after oral administration, thereby hindering effective drug delivery. A novel drug-delivery system of docetaxel (DTX)-based stearic acid (SA)-modified Bletilla striata polysaccharides (BSPs) copolymers was successfully developed. Particle size, zeta potential, encapsulation efficiency (EE), and loading capacity (LC) were determined. The DTX release percentage in vitro was determined using high performance liquid chromatography (HPLC). The hemolysis and in vitro anticancer activity were studied...
December 2, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27917182/expression-of-flavone-synthase-ii-and-flavonoid-3-hydroxylase-is-associated-with-color-variation-in-tan-colored-injured-leaves-of-sorghum
#8
Hiroshi Mizuno, Takayuki Yazawa, Shigemitsu Kasuga, Yuji Sawada, Hiroyuki Kanamori, Yuko Ogo, Masami Yokota Hirai, Takashi Matsumoto, Hiroyuki Kawahigashi
Sorghum (Sorghum bicolor L. Moench) exhibits various color changes in injured leaves in response to cutting stress. Here, we aimed to identify key genes for the light brown and dark brown color variations in tan-colored injured leaves of sorghum. For this purpose, sorghum M36001 (light brown injured leaves), Nakei-MS3B (purple), and a progeny, #7 (dark brown), from Nakei-MS3B × M36001, were used. Accumulated pigments were detected by using high-performance liquid chromatography: M36001 accumulated only apigenin in its light brown leaves; #7 accumulated both luteolin and a small amount of apigenin in its dark brown leaves, and Nakei-MS3B accumulated 3-deoxyanthocyanidins (apigeninidin and luteolinidin) in its purple leaves...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27916761/can-a-p-gp-modulator-assist-in-the-control-of-methotrexate-concentrations-in-the-rat-brain-inhibitory-effects-of-rhodamine-123-a-specific-substrate-for-p-gp-on-methotrexate-excretion-from-the-rat-brain-and-its-optimal-route-of-administration
#9
Naofumi Ogushi, Kazuaki Sasaki, Minoru Shimoda
Although methotrexate (MTX) is mainly transported by reduced folate carrier, P-gp and MRP1 may also be involved in its transport. In our previous study, a potent P-gp and MRP1 modulator, Cyclosporine A, potentiated MTX concentration in rat brain. Since it is important for MTX therapy for brain tumor to clarify which transporter is dominant, we herein determined whether the specific P-gp substrate, rhodamine123 (Rho123), potentiates the transport and retention of MTX in the brain. Rho123 was injected intravenously or intrathecally into rats immediately after injection of MTX...
December 5, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27916403/stability-of-synthetic-cathinones-in-oral-fluid-samples
#10
Briana Miller, Jiyoung Kim, Marta Concheiro
Synthetic cathinones are new stimulant drugs derived from cathinone that have been sold as "legal highs" worldwide. These compounds can elicit powerful effects such as delusions, hallucinations as well as other potentially dangerous behavior. New analogs with varying effects and potencies are constantly introduced in the market to evade legislation, and they are not detected by routine screening and confirmation methods. Oral fluid is an alternative matrix of increasing interest in forensic toxicology. Its collection is non-invasive and easily supervised, and positive drug findings typically reflect recent drug exposure...
December 1, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27916136/a-high-throughput-screening-method-of-bisphenols-bisphenols-digycidyl-ethers-and-their-derivatives-in-dairy-products-by-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#11
Yan Cheng, Xue-Mei Nie, Han-Qiu Wu, Yun-He Hong, Bing-Cheng Yang, Tong Liu, Dan Zhao, Jian-Feng Wang, Gui-Hong Yao, Feng Zhang
A simple and universal analytical method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for high throughput screening of 21 bisphenols, bisphenols digycidyl ethers and their derivatives in dairy products was developed. Response Surface Methodology (RSM) was used to optimize sample preparation conditions based on a quick, easy, cheap, effective, rugged and safe (QuEChERS) method. The analytes were extracted by using 15 mL acetonitrile with 1% acetic acid, and the extracts were further purified by using 190 mg of C18 and 390 mg of PSA...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27915331/screening-of-bioactive-ingredients-in-ligusticum-chuanxiong-hort-for-protection-against-myocardial-ischemia
#12
Xu Liu, Xiuzhong Li, Songgang Ji, Xiaobo Cui, Mingchun Li
BACKGROUND/AIMS: To study the spectrum-effect relationship and effective components of Ligusticum Chuanxiong Hort. (LCH) on the protection of canine myocardial ischemia. METHODS: Fingerprint spectrum of LCH extracts was developed using high performance liquid chromatography (HPLC), and a canine model of acute myocardial ischemia was established by ligating the coronary artery. Bivariate correlation analysis and multivariate regression analysis were used to correlate the pharmacodynamics of LCH extract and its common peaks in HPLC...
December 5, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27915198/a-new-approach-to-the-rapid-separation-of-isomeric-compounds-in-a-silybum-marianum-extract-using-uhplc-core-shell-column-with-f5-stationary-phase
#13
Jakub Fibigr, Dalibor Šatínský, Petr Solich
In this paper, a new ultra-high performance liquid chromatography (UHPLC) method using a core-shell column with a pentafluorophenyl stationary phase for separation of seven active compounds of a Silybum marianum extract was developed and validated. Silymarin, an extract of Silybum marianum, is known for its abilities to protect the liver from toxic substances, hepatitis therapy, and anti-tumour activity. Silymarin is currently being widely used in commercial preparations and herbal teas. Separation of seven compounds contained in the Silybum marianum extract (taxifolin, silychristin, silydianin, silybin A, silybin B, isosilybin A, isosilybin B) and other substances occurring in real samples was performed on the Kinetex 1...
November 27, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27915192/the-profiling-of-the-metabolites-of-hirsutine-in-rat-by-ultra-high-performance-liquid-chromatography-coupled-with-linear-ion-trap-orbitrap-mass-spectrometry-an-improved-strategy-for-the-systematic-screening-and-identification-of-metabolites-in-multi-samples
#14
Jianwei Wang, Peng Qi, Jinjun Hou, Yao Shen, Min Yang, Qirui Bi, Yanping Deng, Xiaojian Shi, Ruihong Feng, Zijin Feng, Wanying Wu, Dean Guo
Drug metabolites identification and construction of metabolic profile are meaningful work for the drug discovery and development. The great challenge during this process is the work of the structural clarification of possible metabolites in the complicated biological matrix, which often resulting in a huge amount data sets, especially in multi-samples in vivo. Analyzing these complex data manually is time-consuming and laborious. The object of this study was to develop a practical strategy for screening and identifying of metabolites from multiple biological samples efficiently...
November 21, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27915078/analgesic-effect-of-coumarins-from-radix-angelicae-pubescentis-is-mediated-by-inflammatory-factors-and-trpv1-in-a-spared-nerve-injury-model-of-neuropathic-pain
#15
Ruili Li, Chao Zhao, Minna Yao, Ying Song, Yin Wu, Aidong Wen
ETHNOPHARMACOLOGICAL RELEVANCE: Coumarins from Radix angelicae pubescentis (CRAP) are a major active component that are isolated from dried roots of Angelica biserrata Yuan et Shan, which has been used clinically to cure headaches for a long period of time, and it is an effective treatment for pain. The aim of the present study was to investigate the analgesic effect of CRAP on a spared nerve injury (SNI) model of neuropathy. MATERIALS AND METHODS: Antinociceptive effects of CRAP were assessed in Sprague-Dawley male rats using a spared nerve injury model of neuropathic pain...
November 30, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27914605/simultaneous-detection-of-recombinant-growth-hormones-in-equine-plasma-by-liquid-chromatography-high-resolution-tandem-mass-spectrometry-for-doping-control
#16
Kin-Sing Wong, George H M Chan, Emmie N M Ho, Terence S M Wan
Growth hormone (GH), or somatotropin, is a protein that may enhance physical performance and facilitate growth and wound healing. For this reason, growth hormones and their recombinant analogues are prohibited in human sports by the World Anti-Doping Agency (WADA) and in horseracing under Article 6 of the International Agreement on Breeding, Racing and Wagering published by the International Federation of Horse Racing Authorities (IFHA). Identifying the illicit use of GHs in both human athletes and racehorses is challenging, especially when differentiating between endogenous and exogenous GHs, and between analogues of GH from different species...
November 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27914321/functional-regulation-of-ginsenoside-biosynthesis-by-rna-interferences-of-a-udp-glycosyltransferase-gene-in-panax-ginseng-and-panax-quinquefolius
#17
Chao Lu, Shoujing Zhao, Guanning Wei, Huijuan Zhao, Qingling Qu
Panax ginseng (Asian ginseng) and Panax quinquefolius (American ginseng) have been used as medicinal and functional herbal remedies worldwide. Different properties of P. ginseng and P. quinquefolius were confirmed not only in clinical findings, but also at cellular and molecular levels. The major pharmacological ingredients of P. ginseng and P. quinquefolius are the triterpene saponins known as ginsenosides. The P. ginseng roots contain a higher ratio of ginsenoside Rg1:Rb1 than that in P. quinquefolius...
November 25, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27913879/termitarium-inhabiting-bacillus-spp-enhanced-plant-growth-and-bioactive-component-in-turmeric-curcuma-longa-l
#18
Ankit Kumar Chauhan, Dinesh Kumar Maheshwari, Shrivardhan Dheeman, Vivek K Bajpai
Curcumin (diferuloyl methane) is the main bioactive component of turmeric (Curcuma longa L.) having remarkable multipotent medicinal and therapeutic applications. Two Bacilli isolated from termitarium soil and identified as Bacillus endophyticus TSH42 and Bacillus cereus TSH77 were used for bacterization of rhizome for raising C. longa ver. suguna for growth and enhancement. Both the strains showed remarkable PGP activities and also chemotactic in nature with high chemotactic index. Turmeric plants bacterized with strains B...
December 2, 2016: Current Microbiology
https://www.readbyqxmd.com/read/27912882/tenuifolin-a-saponin-derived-from-radix-polygalae-exhibits-sleep-enhancing-effects-in-mice
#19
Qing Cao, Yong Jiang, Su-Ying Cui, Peng-Fei Tu, Yue-Mei Chen, Xiao-Li Ma, Xiang-Yu Cui, Yuan-Li Huang, Hui Ding, Jin-Zhi Song, Bin Yu, Zhao-Fu Sheng, Zi-Jun Wang, Ya-Ping Xu, Guang Yang, Hui Ye, Xiao Hu, Yong-He Zhang
BACKGROUND: Radix Polygalae, the dried root of Polygala tenuifolia, has been extensively used as a traditional Chinese medicine for promoting intelligence and tranquilization. Polygalasaponins extracted from the root of P. tenuifolia possess evident anxiolytic and sedative-hypnotic activities. Previous studies have reported that tenuifolin was a major constituent of polygalasaponins. PURPOSE: The currently study aims to investigate the hypnotic effect and possible mechanism of tenuifolin in freely moving mice...
December 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27911900/clinical-and-pharmacological-investigation-of-myotoxicity-in-sri-lankan-russell-s-viper-daboia-russelii-envenoming
#20
Anjana Silva, Christopher Johnston, Sanjaya Kuruppu, Daniela Kneisz, Kalana Maduwage, Oded Kleifeld, A Ian Smith, Sisira Siribaddana, Nicholas A Buckley, Wayne C Hodgson, Geoffrey K Isbister
BACKGROUND: Sri Lankan Russell's viper (Daboia russelii) envenoming is reported to cause myotoxicity and neurotoxicity, which are different to the effects of envenoming by most other populations of Russell's vipers. This study aimed to investigate evidence of myotoxicity in Russell's viper envenoming, response to antivenom and the toxins responsible for myotoxicity. METHODOLOGY AND FINDINGS: Clinical features of myotoxicity were assessed in authenticated Russell's viper bite patients admitted to a Sri Lankan teaching hospital...
December 2016: PLoS Neglected Tropical Diseases
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