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https://www.readbyqxmd.com/read/29667819/synthesis-and-evolution-of-a-tna-aptamer-bearing-7-deaza-7-substituted-guanosine-residues
#1
Hui Mei, Jen-Yu Liao, Randi M Jimenez, Yajun Wang, Saikat Bala, Cailen McCloskey, Christopher Switzer, John C Chaput
In vitro selection experiments carried out on artificial genetic polymers require robust and faithful methods for copying genetic information back and forth between DNA and xeno-nucleic acids (XNA). Previously, we have shown that Kod-RI, an engineered polymerase developed to transcribe DNA templates into threose nucleic acid (TNA), can function with high fidelity in the absence of manganese ions. However, the transcriptional efficiency of this enzyme diminishes greatly when individual templates are replaced with libraries of DNA sequences, indicating that manganese ions are still required for in vitro selection...
April 18, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29663977/development-and-testing-of-the-church-environment-audit-tool
#2
Andrew T Kaczynski, Danielle E Jake-Schoffman, Nathan A Peters, Caroline G Dunn, Sara Wilcox, Melinda Forthofer
OBJECTIVES: In this paper, we describe development and reliability testing of a novel tool to evaluate the physical environment of faith-based settings pertaining to opportunities for physical activity (PA) and healthy eating (HE). METHODS: Tool development was a multistage process including a review of similar tools, stakeholder review, expert feedback, and pilot testing. Final tool sections included indoor opportunities for PA, outdoor opportunities for PA, food preparation equipment, kitchen type, food for purchase, beverages for purchase, and media...
May 1, 2018: American Journal of Health Behavior
https://www.readbyqxmd.com/read/29662693/paediatric-and-obstetric-outcomes-at-a-faith-based-hospital-during-the-100-day-public-sector-physician-strike-in-kenya
#3
Mary Beth Adam, Sarah Muma, Jecinter Achieng Modi, Mardi Steere, Nate Cook, Wayne Ellis, Catherine T Chen, Arianna Shirk, John K Muma Nyagetuba, Erik N Hansen
Published reviews of national physician strikes have shown a reduction in patient mortality. From 5 December 2016 until 14 March 2017, Kenyan physicians in the public sector went on strike leaving only private (not-for-profit and for-profit) hospitals able to offer physician care. We report on our experience at AIC-Kijabe Hospital, a not-for-profit, faith-based Kenyan hospital, before, during and after the 100-day strike was completed by examining patient admissions and deaths in the time periods before, during and after the strike...
2018: BMJ Global Health
https://www.readbyqxmd.com/read/29661749/a-website-supporting-sensitive-religious-and-cultural-advance-care-planning-acptalk-formative-and-summative-evaluation
#4
Amanda Pereira-Salgado, Patrick Mader, Clare O'Callaghan, Leanne Boyd
BACKGROUND: Advance care planning (ACP) promotes conversations about future health care needs, enacted if a person is incapable of making decisions at end-of-life that may be communicated through written documentation such as advance care directives. To meet the needs of multicultural and multifaith populations in Australia, an advance care planning website, ACPTalk, was funded to support health professionals in conducting conversations within diverse religious and cultural populations...
April 16, 2018: JMIR Research Protocols
https://www.readbyqxmd.com/read/29660942/the-role-of-the-gut-microbiota-in-the-metabolism-of-polyphenols-as-characterized-by-gnotobiotic-mice
#5
Giulio Maria Pasinetti, Risham Singh, Susan Westfall, Francis Herman, Jeremiah Faith, Lap Ho
A growing body of experimental data suggests that microbes in the gut influence behavior and can alter brain physiology and neurochemistry. Although promising, researchers are only starting to understand the potential of the gut microbiota for use in neurological disease. Recent evidence demonstrated that gastrointestinal activities are linked to mood disorders such as anxiety, depression, and most recently, cognitive functions in age-related neurodegenerative disorders. Studies from our group and others are uncovering new evidence suggesting that the gut microbiota plays a crucial role in the metabolism and bioavailability of certain dietary compounds and synthetic drugs...
April 11, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29658277/cavitation-enhancement-increases-the-efficiency-and-consistency-of-chromatin-fragmentation-from-fixed-cells-for-downstream-quantitative-applications
#6
Anna M Chiarella, Austin L Quimby, Marjan Mehrab-Mohseni, Brian Velasco, Sandeep K Kasoji, Ian Davis, Paul A Dayton, Nathaniel A Hathaway, Samantha Pattenden
One of the most sensitive, time consuming, and variable steps of chromatin immunoprecipitation (ChIP) is chromatin sonication. Traditionally, this process can take hours to properly sonicate enough chromatin for multiple ChIP assays. Further, the length of sheared DNA is often inconsistent. In order to faithfully measure chemical and structural changes at the chromatin level, sonication needs to be reliable. Thus, chromatin fragmentation by sonication represents a significant bottleneck to downstream quantitative analysis...
April 16, 2018: Biochemistry
https://www.readbyqxmd.com/read/29656913/faith-activity-and-nutrition-randomized-dissemination-and-implementation-study-countywide-adoption-reach-and-effectiveness
#7
Sara Wilcox, Ruth P Saunders, Andrew T Kaczynski, Melinda Forthofer, Patricia A Sharpe, Cheryl Goodwin, Margaret Condrasky, Vernon L Kennedy, Danielle E Jake-Schoffman, Deborah Kinnard, Brent Hutto
INTRODUCTION: Faith-based organizations can contribute to improving population health, but few dissemination and implementation studies exist. This paper reports countywide adoption, reach, and effectiveness from the Faith, Activity, and Nutrition dissemination and implementation study. DESIGN: This was a group-randomized trial. Data were collected in 2016. Statistical analyses were conducted in 2017. SETTING/PARTICIPANTS: Churches in a rural, medically underserved county in South Carolina were invited to enroll, and attendees of enrolled churches were invited to complete questionnaires (n=1,308 participated)...
April 9, 2018: American Journal of Preventive Medicine
https://www.readbyqxmd.com/read/29651379/molecular-cloning-and-characterization-of-pif-gene-from-pearl-mussel-hyriopsis-cumingii-and-the-gene-expression-analysis-during-pearl-formation
#8
Rui Zhang, Mengting Qin, Jie Shi, Lu Tan, Jiamin Xu, Zhenyan Tian, Yuhui Wu, Yuxuan Li, Yitian Li, Ning Wang
In the present study, the Pif gene of the freshwater pearl aquaculture mussel, Hyriopsis cumingii (HcPif) was successfully cloned and functionally characterized. The full sequence of HcPif gene consists of 3415 base pairs, which putatively encode two proteins, HcPif90 and HcPif80. A sequence analysis revealed that HcPif contained a von Willebrand factor type A domain and a chitin-binding domain, and shared many functional residues with other Pif homologues. A highly conserved sequence, FKGLDEIELML, at the C-terminus of Pif80s was identified as the key functional site...
April 2018: 3 Biotech
https://www.readbyqxmd.com/read/29629761/small-gd-iii-tags-for-gd-iii-gd-iii-distance-measurements-in-proteins-by-epr-spectroscopy
#9
Georgia Prokopiou, Michael D Lee, Alberto Collauto, Elwy H Abdelkader, Thorsten Bahrenberg, Akiva Feintuch, Marie Ramirez-Cohen, Jessica Clayton, James D Swarbrick, Bim Graham, Gottfried Otting, Daniella Goldfarb
The C7-Gd and C8-Gd tags are compact hydrophilic cyclen-based lanthanide tags for conjugation to cysteine residues in proteins. The tags are enantiomers, which differ in the configuration of the 2-hydroxylpropyl pendant arms coordinating the lanthanide ion. Here, we report the electron paramagnetic resonance (EPR) performance of the C7-Gd ( S configuration) and C8-Gd ( R configuration) tags loaded with Gd(III) on two mutants of the homodimeric ERp29 protein. The W-band EPR spectra were found to differ between the tags in the free state and after conjugation to the protein...
April 9, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29621068/late-life-socioeconomic-status-and-hypertension-in-an-aging-cohort-the-atherosclerosis-risk-in-communities-study
#10
M Maya McDoom, Priya Palta, Priya Vart, Stephen Juraschek, Anna Kucharska-Newton, Anna V Diez Roux, Josef Coresh
OBJECTIVE: To investigate the association between individual and area-level socioeconomic status and hypertension risk among individuals later in life. METHODS: We used Cox proportional hazards models to examine the association of socioeconomic status with incident hypertension using race-specific neighborhood socioeconomic status, median household income, and education among 3372 participants (mean age, 61 years) from the Atherosclerosis Risk in Communities Study at Visit 4 (1996-1998)...
April 4, 2018: Journal of Hypertension
https://www.readbyqxmd.com/read/29619835/augmented-pairwise-additive-interaction-model-for-lateral-adsorbate-interactions-the-no-co-reaction-system-on-rh-100-and-rh-111
#11
Lu Tan, Liangliang Huang, Yingchun Liu, Qi Wang
Lateral adsorbate interactions have been acknowledged to play an important role in heterogeneous catalytic kinetics. To quantify such energies efficiently and accurately, a lattice-gas-based augmented pairwise additive interaction model was proposed. The model Hamiltonian is defined as the summation over all isolated binding energies and all pairwise interactions, with the pairwise interaction used for each adsorbate pair consisting of the corresponding isolated pairwise interaction and some needed modification terms if the surroundings meet specific conditions...
April 5, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29617781/impact-of-counseling-received-by-adolescents-undergoing-voluntary-medical-male-circumcision-on-knowledge-and-sexual-intentions
#12
Michelle R Kaufman, Eshan U Patel, Kim H Dam, Zoe R Packman, Lynn M Van Lith, Karin Hatzold, Arik V Marcell, Webster Mavhu, Catherine Kahabuka, Lusanda Mahlasela, Emmanuel Njeuhmeli, Kim Seifert Ahanda, Getrude Ncube, Gissenge Lija, Collen Bonnecwe, Aaron A R Tobian
Background: Little is known regarding the impact of counseling delivered during voluntary medical male circumcision (VMMC) services on adolescents' human immunodeficiency virus (HIV) knowledge, VMMC knowledge, or post-VMMC preventive sexual intentions. This study assessed the effect of counseling on knowledge and intentions. Methods: Surveys were conducted with 1293 adolescent clients in 3 countries (South Africa, n = 299; Tanzania, n = 498; Zimbabwe, n = 496). Adolescents were assessed on HIV and VMMC knowledge-based items before receiving VMMC preprocedure counseling and at a follow-up survey approximately 10 days postprocedure...
April 3, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29615084/the-association-between-self-rated-health-and-social-environments-health-behaviors-and-health-outcomes-a-structural-equation-analysis
#13
Bevan Adrian Craig, Darren Peter Morton, Peter John Morey, Lillian Marton Kent, Alva Barry Gane, Terry Leslie Butler, Paul Meredith Rankin, Kevin Ross Price
BACKGROUND: The factors shaping the health of the current generation of adolescents are multi-dimensional and complex. The purpose of this study was to explore the determinants of self-rated health (SRH) of adolescents attending a faith-based school system in Australia. METHODS: A total of 788 students attending 21 Seventh-day Adventist schools in Australia responded to a health and lifestyle survey that assessed SRH as well as potential determinants of SRH including the health outcomes mental health, vitality, body mass index (BMI), select health behaviors, social factors and personal demographics...
April 3, 2018: BMC Public Health
https://www.readbyqxmd.com/read/29609487/3d-printing-processes-for-photocurable-polymeric-materials-technologies-materials-and-future-trends
#14
Gabriele Taormina, Corrado Sciancalepore, Massimo Messori, Federica Bondioli
The aim of this review is a faithful report of the panorama of solutions adopted to fabricate a component using vat photopolymerization (VP) processes. A general overview on additive manufacturing and on the different technologies available for polymers is given. A comparison between stereolithography and digital light processing is also presented, with attention to different aspects and to the advantages and limitations of both technologies. Afterward, a quick overview of the process parameters is given, with an emphasis on the necessities and the issues associated with the VP process...
April 1, 2018: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/29605081/fcs-analysis-of-protein-mobility-on-lipid-monolayers
#15
Alena Khmelinskaia, Jonas Mücksch, Franco Conci, Grzegorz Chwastek, Petra Schwille
In vitro membrane model systems are used to dissect complex biological phenomena under controlled unadulterated conditions. In this context, lipid monolayers are a powerful tool to particularly study the influence of lipid packing on the behavior of membrane proteins. Here, monolayers deposited in miniaturized fixed area-chambers, which require only minute amounts of protein, were used and shown to faithfully reproduce the characteristics of Langmuir monolayers. This assay is ideally suited to be combined with single-molecule sensitive fluorescence correlation spectroscopy (FCS) to characterize diffusion dynamics...
March 29, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29602091/developing-religiously-tailored-health-messages-for-behavioral-change-introducing-the-reframe-reprioritize-and-reform-3r-model
#16
Aasim I Padela, Sana Malik, Milkie Vu, Michael Quinn, Monica Peek
RATIONALE: As community health interventions advance from being faith-placed to authentically faith-based, greater discussion is needed about the theory, practice, and ethics of delivering health messages embedded within a religious worldview. While there is much potential to leverage religion to promote health behaviors and improve health outcomes, there is also a risk of co-opting religious teachings for strictly biomedical ends. OBJECTIVE: To describe the development, implementation, and ethical dimensions of a conceptual model for religiously-tailoring health messages...
March 21, 2018: Social Science & Medicine
https://www.readbyqxmd.com/read/29600517/the-impact-of-length-of-stay-on-recovery-measures-in-faith-based-addiction-treatment
#17
Mary Lashley
OBJECTIVE: To determine the impact of length of stay among homeless men in faith-based residential addictions recovery on physical activity, depression, self-esteem, and nicotine dependence. DESIGN AND SAMPLE: A time series design was utilized to measure changes in the four quality measures at program entry and at 3, 6, and 9 months following admission. The sample consisted of 175 homeless residents enrolled in a faith-based residential recovery program. MEASURES: Paired t tests were used to determine the change in average instrument response from admission to each follow-up period...
March 30, 2018: Public Health Nursing
https://www.readbyqxmd.com/read/29595596/accredited-health-department-partnerships-to-improve-health-an-analysis-of-community-health-assessments-and-improvement-plans
#18
Jessica Kronstadt, Chinecherem Chime, Bulbul Bhattacharya, Nicole Pettenati
CONTEXT: The Public Health Accreditation Board (PHAB) Standards & Measures require the development and updating of collaborative community health assessments (CHAs) and community health improvement plans (CHIPs). OBJECTIVE: The goal of this study was to analyze the CHAs and CHIPs of PHAB-accredited health departments to identify the types of partners engaged, as well as the objectives selected to measure progress toward improving community health. DESIGN: The study team extracted and coded data from documents from 158 CHA/CHIP processes submitted as part of the accreditation process...
May 2018: Journal of Public Health Management and Practice: JPHMP
https://www.readbyqxmd.com/read/29593542/apigenin-and-luteolin-attenuate-the-breaching-of-mda-mb231-breast-cancer-spheroids-through-the-lymph-endothelial-barrier-in-vitro
#19
Junli Hong, Adryan Fristiohady, Chi H Nguyen, Daniela Milovanovic, Nicole Huttary, Sigurd Krieger, Junqiang Hong, Silvana Geleff, Peter Birner, Walter Jäger, Ali Özmen, Liselotte Krenn, Georg Krupitza
Flavonoids, present in fruits, vegetables and traditional medicinal plants, show anticancer effects in experimental systems and are reportedly non-toxic. This is a favorable property for long term strategies for the attenuation of lymph node metastasis, which may effectively improve the prognostic states in breast cancer. Hence, we studied two flavonoids, apigenin and luteolin exhibiting strong bio-activity in various test systems in cancer research and are readily available on the market. This study has further advanced the mechanistic understanding of breast cancer intravasation through the lymphatic barrier...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29593487/hearing-the-sound-in-the-brain-influences-of-different-eeg-references
#20
Dan Wu
If the scalp potential signals, the electroencephalogram (EEG), are due to neural "singers" in the brain, how could we listen to them with less distortion? One crucial point is that the data recording on the scalp should be faithful and accurate, thus the choice of reference electrode is a vital factor determining the faithfulness of the data. In this study, music on the scalp derived from data in the brain using three different reference electrodes were compared, including approximate zero reference-reference electrode standardization technique (REST), average reference (AR), and linked mastoids reference (LM)...
2018: Frontiers in Neuroscience
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