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https://www.readbyqxmd.com/read/29929208/dna-barcoding-for-the-identification-of-phyllanthus-taxa-used-medicinally-in-brazil
#1
Peter W Inglis, Lorena R Mata, Marcos José da Silva, Roberto F Vieira, Rosa de B N Alves, Dijalma B Silva, Vânia C R Azevedo
Plants of the genus Phyllanthus, principally Phyllanthus amarus, Phyllanthus urinaria, Phyllanthus niruri , and Phyllanthus tenellus, are used in Brazilian folk medicine to treat kidney stones as well as other ailments, where the latter two species are listed in the Brazilian Pharmacopeia as quebra-pedra ( stone-breaker ). However, only P. niruri has been shown to be effective in a clinical setting. Nuclear ribosomal internal transcribed spacer (ITS1 - 5.8S rRNA-ITS2), internal transcribed spacer 2, and chloroplasts rbc L, mat K, psb A- trn H, trn L, and trn L- trn F were screened for their potential as DNA barcodes for the identification of 48 Phyllanthus taxa in Brazilian medicinal plant germplasm banks and in "living pharmacies"...
June 21, 2018: Planta Medica
https://www.readbyqxmd.com/read/29928793/direct-assessment-of-nano-confined-water-in-2d-ti3c2-mxene-electrode-interspaces-by-a-surface-acoustic-technique
#2
Netanel Shpigel, Mikhael D Levi, Sergey Sigalov, Tyler S Mathis, Yury Gogotsi, Doron Aurbach
Although significant progress has been achieved in understanding of ion-exchange mechanisms in the new family of 2D transition metal carbides and nitrides known as MXenes, direct gravimetric assessment of water insertion into the MXene interlayer spaces and mesopores has not been reported so far. Concurrently, the latest research on MXene and Birnessite electrodes shows that nanoconfined water dramatically improves their gravimetric capacity and rate capability . Hence quantification of the amount of confined water in solvated electrodes is becoming an important goal of energy-related research...
June 21, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29928201/efficacy-and-tolerability-of-topical-green-tea-extract-polyphenon-e-application-in-a-therapy-resistant-plantar-wart
#3
Giancarlo Meloni, Massimo Milani
Plantar warts account for 30% of all cutaneous warts. These lesions could be very painful, especially if the lesion is located over pressure sites such as the metatarsal head. Plantar wart treatment remains a challenging therapeutic problem. A 67-year-old immunocompetent nonsmoking man presented with a large mosaic plantar wart on his right foot. The lesion had been present for 5 years. Several cryotherapy sessions (a total of 6 procedures) had been performed with no success. The lesion was therefore treated with a 5-fluorouracil (5-FU) regimen and then with a topical combination of 5-FU and salicylic acid, but also these approaches failed...
May 2018: Case Reports in Dermatology
https://www.readbyqxmd.com/read/29928110/theaflavin-alleviates-inflammatory-response-and-brain-injury-induced-by-cerebral-hemorrhage-via-inhibiting-the-nuclear-transcription-factor-kappa-%C3%AE-related-pathway-in-rats
#4
Guanglei Fu, Hua Wang, Youli Cai, Hui Zhao, Wenjun Fu
Objective: Intracerebral hemorrhage (ICH) is one of the most common acute cerebrovascular diseases with high mortality. Numerous studies have shown that inflammatory response played an important role in ICH-induced brain injury. Theaflavin (TF) extracted from black tea has various biological functions including anti-inflammatory activity. In this study, we investigated whether TF could inhibit ICH-induced inflammatory response in rats and explored its mechanism. Materials and methods: ICH rat models were induced with type VII collagenase and pretreated with TF by gavage in different doses (25 mg/kg-100 mg/kg)...
2018: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29926929/sulphite-free-lamb-burger-meat-antimicrobial-and-antioxidant-properties-of-green-tea-and-carvacrol
#5
M Bellés, V Alonso, P Roncalés, J A Beltrán
BACKGROUND: Sulphite is commonly used to preserve lamb burger meat in the European Union. Nevertheless, its consumption has been related to certain health problems which have increased consumer demand of free-sulphite products. Natural compounds with antioxidant and antimicrobial properties may be a feasible alternative to preserve lamb burger meat. This study evaluated the antimicrobial and antioxidant properties of carvacrol, green tea and their combination in preserving lamb burger meat...
June 21, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29926702/different-catabolism-pathways-triggered-by-various-1-methylxanthines-in-caffeine-tolerant-bacterium-pseudomonas-putida-ct25-isolated-from-tea-garden-soil
#6
Yi-Xiao Ma, Xiao-Han Wu, Hui-Shi Wu, Zhan-Bo Dong, Jian-Hui Ye, Xin-Qiang Zheng, Yue-Rong Liang, Jianliang Lu
Degradation efficiency and catabolism pathways of the different methylxanthines (MXs) in isolated caffeine-tolerant strain Pseudomonas putida CT25 were comprehensively studied. The results showed that degradation efficiency of various MXs varied with number and position of the methyl-groups on the molecule ( i.e. , xanthine > 7-methylxanthine ≈ theobromine > caffeine > theophylline > 1-methylxanthine). Multiple MXs catabolism pathways coexisted in strain CT25 and a different pathway would be triggered by various MXs...
May 11, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29925916/needleless-transcutaneous-electrical-acustimulation-a-pilot-study-evaluating-improvement-in-post-operative-recovery
#7
Bo Zhang, Feng Xu, Pingping Hu, Mingyuan Zhang, Kehui Tong, Gang Ma, Yuemei Xu, Liang Zhu, Jiande D Z Chen
BACKGROUND: Functional gastrointestinal disturbance occurs after abdominal surgeries and could last for an extended period of time in some cases. This study was designed (1) to evaluate the effects of needleless transcutaneous electrical acustimulation (TEA) on postoperative recovery, and (2) to investigate the mechanisms involving autonomic function in postoperative patients after removal of gastrointestinal cancers. METHODS: Forty-two patients (33 male, age: 69...
June 21, 2018: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/29925724/influence-of-regional-anesthesia-on-the-rate-of-chronic-postthoracotomy-pain-syndrome-in-lung-cancer-patients
#8
Victoria Khoronenko, Danil Baskakov, Marc Leone, Anna Malanova, Andrey Ryabov, Oleg Pikin, Maksim Golovashchenko
PURPOSE: Our study aimed to assess whether the type of regional anesthesia influenced the incidence of chronic postthoracotomy pain syndrome (CPTPS). METHODS: This was a prospective, randomized study that included 300 patients undergoing lung cancer resection using thoracotomy. They were randomized into three groups: paravertebral nerve block (PVB), thoracic epidural anesthesia (TEA), and intercostal nerve block (INB). General anesthesia was similar in the groups...
June 20, 2018: Annals of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29923153/black-tea-affects-obesity-by-reducing-nutrient-intake-and-activating-amp-activated-protein-kinase-in-mice
#9
Shunshun Pan, Xuming Deng, Shili Sun, Xingfei Lai, Lingli Sun, Qiuhua Li, Limin Xiang, Lingzhi Zhang, Yahui Huang
The effects of certain tea components on the prevention of obesity in humans have been reported recently. However, whether Yinghong NO. 9 black tea consumption has beneficial effects on obesity are not known. Here, we obtained a Yinghong NO. 9 black tea infusion (Y9 BTI) and examined the anti-obesity effects of its oral administration. ICR mice were fed a standard diet supplemented with Y9 BTI at 0.5, 1.0, or 2.0 g/kg body weight for two weeks, and the body weight were recorded. HE staining was used to evaluate the effect of Y9 BTI on mice liver...
June 19, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29921872/caffeine-inducible-gene-switches-controlling-experimental-diabetes
#10
Daniel Bojar, Leo Scheller, Ghislaine Charpin-El Hamri, Mingqi Xie, Martin Fussenegger
Programming cellular behavior using trigger-inducible gene switches is integral to synthetic biology. Although significant progress has been achieved in trigger-induced transgene expression, side-effect-free remote control of transgenes continues to challenge cell-based therapies. Here, utilizing a caffeine-binding single-domain antibody we establish a caffeine-inducible protein dimerization system, enabling synthetic transcription factors and cell-surface receptors that enable transgene expression in response to physiologically relevant concentrations of caffeine generated by routine intake of beverages such as tea and coffee...
June 19, 2018: Nature Communications
https://www.readbyqxmd.com/read/29921123/metabolomics-investigation-reveals-8-c-n-ethyl-2-pyrrolidinone-substituted-flavan-3-ols-are-potential-marker-compounds-of-stored-white-teas
#11
Weidong Dai, Junfeng Tan, Meiling Lu, Yin Zhu, Pengliang Li, Qunhua Peng, Li Guo, Yue Zhang, Dongchao Xie, Zhengyan Hu, Zhi Lin
White teas of different stored ages have varied flavor, bioactivity, and commercial value. In this study, liquid chromatography-mass spectrometry (LC-MS)-based metabolomics investigation revealed that there are distinct differences among the compound patterns of Baihaoyinzhen (BHYZ) and Baimudan (BMD) white teas with various storage durations. The levels of flavan-3-ols, procyanidins, theasinensins, theaflavins, flavonol-O-glycosides, flavone-C-glycosides, and most of the amino acids were reduced after long-term (>4 years) storage...
June 19, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29920918/influence-of-composite-type-and-light-irradiance-on-color-stability-after-immersion-in-different-beverages
#12
Victória Alberton Da Silva, Simone Alberton Da Silva, Oscar E Pecho, Atais Bacchi
OBJECTIVE: The aim of this study was to evaluate the color stability of two resin-based composites photo-activated by two light curing units (LCU) with different irradiances. MATERIALS AND METHODS: Hundred disc-shaped specimens (2-mm thick) of a nanofilled (FZ- FiltekTM Z350 XT, 3M ESPE) and a microhybrid (ED-Empress® Direct, Ivoclar Vivadent) composites were photo-activated with two LCU: Valo® Cordless, Ultradent (VA-1800 mW/cm2 ) or Radii-cal, SDI (RA-900 mW/cm2 )...
June 19, 2018: Journal of Esthetic and Restorative Dentistry
https://www.readbyqxmd.com/read/29920704/development-and-validation-of-a-high-throughput-assay-for-the-quantification-of-multiple-green-tea-derived-catechins-in-human-plasma
#13
Deborah H Mawson, Keon L Jeffrey, Philip Teale, Philip B Grace
A rapid, accurate and robust method for the determination of catechin (C), epicatechin (EC), gallocatechin (GC), epigallocatechin (EGC), catechin gallate (Cg), epicatechin gallate (ECg), gallocatechin gallate (GCg) and epigallocatechin gallate (EGCg) concentrations in human plasma has been developed. The method utilises protein precipitation following enzyme hydrolysis, with chromatographic separation and detection using reversed-phase liquid chromatography - tandem mass spectrometry (LC-MS/MS). Traditional issues such as lengthy chromatographic run times, sample and extract stability, and lack of suitable internal standards have been addressed...
June 19, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29920648/botanicals-and-hepatotoxicity
#14
Marina M Roytman, Peter Poerzgen, Victor Navarro
The use of botanicals, often in the form of multi-ingredient herbal dietary supplements (HDS), has grown tremendously in the past three decades despite their unproven efficacy. This is paralleled by an increase in dietary supplement-related health complications, notably hepatotoxicity. This article reviews the demographics and motivations of dietary supplement (DS) consumers and the regulatory framework for DS in the US and other developed countries. It examines in detail three groups of multi-ingredient HDS associated with hepatotoxicity: OxyElite Pro (two formulations), green tea extract-based DS, and "designer anabolic steroids...
June 19, 2018: Clinical Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29916708/involvement-of-three-csrhm-genes-from-camellia-sinensis-in-udp-rhamnose-biosynthesis
#15
Xinlong Dai, Guifu Zhao, Tianming Jiao, Yingling Wu, Xinmin Li, Kan Zhou, Liping Gao, Tao Xia
UDP-rhamnose synthase (RHM), the branch-point enzyme controlling the nucleotide sugar interconversion pathway, converts UDP-D-glucose into UDP-rhamnose. As a rhamnose residue donor, UDP-L-rhamnose is essential for the biosynthesis of pectic polysaccharides and secondary metabolites in plants. In this study, three CsRHM genes from tea plants (Camellia sinensis) were cloned and characterized. Enzyme assays showed that three recombinant proteins displayed RHM activity and were involved in the biosynthesis of UDP-rhamnose in vitro...
June 19, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29915999/fampridine-is-a-substrate-and-inhibitor-of-human-oct2-but-not-of-human-mate1-or-mate2k
#16
Guangqing Xiao, Christopher Rowbottom, Carri Boiselle, Liang-Shang Gan
PURPOSE: The renal clearance of fampridine (Fampyra®, or Ampyra®) significantly exceeds the glomerular filtration rate, suggesting active renal secretion is likely the major elimination pathway. The goal of this study was to identify the renal transporters that are involved in the renal active secretion, and elucidate the active renal secretion mechanism of fampridine. METHODS: The uptake of fampridine to HEK-293 cells overexpressing human OCT2, MATE1 or MATE2K was determined in the absence and presence of Cimetidine, the prototypical inhibitor of the transporters...
June 18, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29915604/gene-discovery-of-characteristic-metabolic-pathways-in-the-tea-plant-camellia-sinensis-using-omics-based-network-approaches-a-future-perspective
#17
REVIEW
Shihua Zhang, Liang Zhang, Yuling Tai, Xuewen Wang, Chi-Tang Ho, Xiaochun Wan
Characteristic secondary metabolites, including flavonoids, theanine and caffeine, in the tea plant ( Camellia sinensis ) are the primary sources of the rich flavors, fresh taste, and health benefits of tea. The decoding of genes involved in these characteristic components is still significantly lagging, which lays an obstacle for applied genetic improvement and metabolic engineering. With the popularity of high-throughout transcriptomics and metabolomics, 'omics'-based network approaches, such as gene co-expression network and gene-to-metabolite network, have emerged as powerful tools for gene discovery of plant-specialized (secondary) metabolism...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29915390/gallic-acid-improves-cardiac-dysfunction-and-fibrosis-in-pressure-overload-induced-heart-failure
#18
Li Jin, Simei Sun, Yuhee Ryu, Zhe Hao Piao, Bin Liu, Sin Young Choi, Gwi Ran Kim, Hyung-Seok Kim, Hae Jin Kee, Myung Ho Jeong
Gallic acid is a trihydroxybenzoic acid found in tea leaves and some plants. Here, we report the effect of gallic acid on cardiac dysfunction and fibrosis in a mouse model of pressure overload-induced heart failure and in primary rat cardiac fibroblasts, and compare the effects of gallic acid with those of drugs used in clinics. Gallic acid reduces cardiac hypertrophy, dysfunction, and fibrosis induced by transverse aortic constriction (TAC) stimuli in vivo and transforming growth factor β1 (TGF-β1) in vitro...
June 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29914623/efficacy-of-tea-catechin-rich-beverages-to-reduce-abdominal-adiposity-and-metabolic-syndrome-risks-in-obese-and-overweight-subjects-a-pooled-analysis-of-6-human-trials
#19
REVIEW
Masanobu Hibi, Hideto Takase, Masaki Iwasaki, Noriko Osaki, Yoshihisa Katsuragi
This post hoc pooled analysis assessed the effectiveness of green tea catechins (GTC) to reduce the risk of metabolic syndrome (MetS) associated with abdominal fat reduction, because previous findings are unclear. Data were pooled from six human trials (n=921, 505 men) comparing the effects of GTC-containing beverages (540-588 mg GTC/beverage) and a placebo beverage. Outcome measures were abdominal fat [total fat area (TFA), visceral fat area (VFA), subcutaneous fat area (SFA)], and MetS risk. We estimated mean changes from baseline and calculated confidence intervals (CI) to assess reductions in abdominal fat accumulation and MetS improvement...
July 2018: Nutrition Research
https://www.readbyqxmd.com/read/29914362/effects-of-vitro-sucrose-on-quality-components-of-tea-plants-camellia-sinensis-based-on-transcriptomic-and-metabolic-analysis
#20
Yumei Qian, Shuxiang Zhang, Shengbo Yao, Jinxin Xia, Yanzhi Li, Xinlong Dai, Wenzhao Wang, Xiaolan Jiang, Yajun Liu, Mingzhuo Li, Liping Gao, Tao Xia
BACKGROUND: Tea plants [Camellia sinensis (L.) O. Kuntze] can produce one of the three most widely popular non-alcoholic beverages throughout the world. Polyphenols and volatiles are the main functional ingredients determining tea's quality and flavor; however, the biotic or abiotic factors affecting tea polyphenol biosynthesis are unclear. This paper focuses on the molecular mechanisms of sucrose on polyphenol biosynthesis and volatile composition variation in tea plants. RESULTS: Metabolic analysis showed that the total content of anthocyanins, catechins, and proanthocyanidins(PAs) increased with sucrose, and they accumulated most significantly after 14 days of treatment...
June 18, 2018: BMC Plant Biology
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