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https://www.readbyqxmd.com/read/28640662/using-probiotics-in-clinical-practice-where-are-we-now-a-review-of-existing-meta-analyses
#1
Mariangela Rondanelli, Milena Anna Faliva, Simone Perna, Attilio Giacosa, Gabriella Peroni, Anna Maria Castellazzi
The scientific literature has demonstrated that probiotics have a broad spectrum of activity, although often the results are contradictory. This study provides a critical overview of the current meta-analyses that have evaluated the efficacy of probiotics in physiological and pathological conditions, such as metabolic disease, antibiotic-associated and Clostridium difficile-associated diarrhea, IBS, constipation, IBD, chemotherapy-associated diarrhea, respiratory tract infection, ventilator-associated pneumonia, NAFLD, liver encephalopathy, periodontitis, depression, vaginosis, urinary tract infections, pancreatitis, incidence of ventilator-associated pneumonia, hospital infection and stay in ICU, mortality of post-trauma patients, necrotising enterocolitis in premature infants...
June 22, 2017: Gut Microbes
https://www.readbyqxmd.com/read/28618852/low-vitamin-d-is-associated-with-infections-and-proinflammatory-cytokines-during-pregnancy
#2
Christine C Akoh, Eva K Pressman, Elizabeth Cooper, Ruth Anne Queenan, Julie Pillittere, Kimberly O O'Brien
Vitamin D is known to regulate innate and adaptive immune processes at the cellular level, but the role of vitamin D status on associated inflammatory processes across pregnancy is unclear. Our primary objective was to evaluate the relationships between serum biomarkers of inflammation (interleukin [IL]-6, IL-10, tumor necrosis factor [TNF]-α), acute-phase proteins (C-reactive protein and hepcidin) and vitamin D status, 25-hydroxyvitamin D (25(OH)D) and 1,25-dihydroxyvitamin D (1,25(OH)2D), measured across pregnancy and in the neonate at birth...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28617533/cervical-carcinogenesis-bacterial-vaginosis-hpv-mrna-test-and-relapse-of-cin2-after-loop-electrosurgical-excision-procedure-leep
#3
A Frega, G Lavini, A Guarino, V Giovanale, A Lukic, P Bianchi, G Cozza, R Marziani, A D'Ambrosio, D French, D Caserta
OBJECTIVE: The aim of the study was to evaluate the relationship between bacterial vaginosis (BV) and relapse of cervical intraepithelial neoplasia grade 2 or more (CIN2+) after Loop electrosurgical excision procedure (LEEP). PATIENTS AND METHODS: One hundred four patients who underwent LEEP for CIN2+ were followed up every six months for three years. Fifty-three were negative for BV and fifty-one were positive. Each clinical control included Pap test, colposcopy, Amsel criteria test, HPV-DNA, and HPV-mRNA test...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28610416/bacterial-vaginosis-and-cervical-intraepithelial-neoplasia-is-there-an-association-or-is-co-existence-incidental
#4
Pushpa Sodhani, Sanjay Gupta, Ruchika Gupta, Ravi Mehrotra
Objectives: To determine associations, if any, of bacterial vaginosis with cervical pre-neoplastic lesions and evaluate any effects of sub-categorization of smears with bacterial vaginosis. Methods: All cervico-vaginal smears reported as positive for bacterial vaginosis over a five-year period were reviewed and sub-categorized into ‘type I (dysbacteriosis)’ and ‘type II (pure Gardenerella infection)’ smears by two cytopathologists (PS, SG). The proportion of smears with healthy flora and pre-neoplastic lesions was compared with those having bacterial vaginosis in conjunction with such changes...
May 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28604063/vaginal-self-sampling-as-a-diagnosis-tool-in-low-income-countries-and-potential-applications-for-exploring-the-infectious-causes-of-miscarriage
#5
Jean-Christophe Lagier, Nafissatou Diagne, Florence Fenollar, Catherine Tamalet, Cheikh Sokhna, Didier Raoult
Gynecological health is a challenge in low-income countries. Personal opposition to perineal examination has been overcome by the use of vaginal self-sampling. Here, we review the use of this procedure notably in low-income countries and the main infectious causes of miscarriage. Vaginal self-sampling was mainly used for human papillomavirus detection but also to detect microorganisms causing sexually transmitted infections or bacterial vaginosis. 58 studies have been performed in low-resource countries, mainly studies performed to detect human papillomavirus in urban and peri-urban areas and demonstrating excellent acceptability...
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28603641/dakarella-massiliensis-gen-nov-sp-nov-strain-nd3-t-a-new-bacterial-genus-isolated-from-the-female-genital-tract
#6
N Dione, J Rathored, E Tomei, J-C Lagier, S Khelaifia, C Robert, F Bretelle, D Raoult, P-E Fournier, F Fenollar
Strain ND3(T) was isolated from the genital tract of a 28-year-old woman with bacterial vaginosis. This strain exhibited a 16S rRNA gene sequence similarity of 92.4% with Sutterella wadsworthensis, the phylogenetically closest species with standing in nomenclature. Strain ND3(T) was a strictly anaerobic Gram-negative rod and member of the family Sutterellaceae. It exhibited a genome of 2 476 884 bp containing 2175 protein-coding and 62 RNA genes. On the basis of these data, we propose the creation of 'Dakarella massiliensis' sp...
July 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28600771/multiplex-peptide-nucleic-acid-fluorescence-in-situ-hybridization-pna-fish-for-diagnosis-of-bacterial-vaginosis
#7
Antonio Machado, Nuno Cerca
Fluorescence in situ hybridization (FISH) is a molecular method used to identify and quantify microorganisms in a wide range of samples. This technique combines the simplicity of microscopic observation and the specificity of DNA/rRNA hybridization, allowing detection of selected bacterial species and morphologic visualization. Here, we describe a quantitative molecular diagnosis of bacterial vaginosis, based on the classical Nugent score. Our probes are able to differentiate Lactobacillus spp. and Gardnerella vaginalis from the other undefined bacterial species considered in the Nugent score...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28594779/clinical-validation-of-a-test-for-the-diagnosis-of-vaginitis
#8
Charlotte A Gaydos, Sajo Beqaj, Jane R Schwebke, Joel Lebed, Bonnie Smith, Thomas E Davis, Kenneth H Fife, Paul Nyirjesy, Timothy Spurrell, Dorothy Furgerson, Jenell Coleman, Sonia Paradis, Charles K Cooper
OBJECTIVE: Vaginitis may be diagnosed as bacterial vaginosis, vulvovaginal candidiasis, trichomoniasis, or coinfection. A new molecular test assays the vaginal microbiome and organisms that cause three common infections. The objective of the trial was to evaluate the clinical accuracy of the investigational test for vaginal swabs collected by patients (self) or clinicians. The primary and secondary outcomes were to compare the investigational test with reference methods for the three most common causes of vaginitis and compare clinician-collected with self-collected swabs...
June 6, 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28593917/vitamin-d-deficiency-in-pregnancy-at-term-risk-factors-and-pregnancy-outcomes
#9
Chui Ling Lee, Beng Kwang Ng, Loo Ling Wu, Fook Choe Cheah, Hanita Othman, Nor Azlin Mohamed Ismail
Background Vitamin D deficiency during pregnancy is a worldwide epidemic. The aim of this study was to determine the prevalence of vitamin D deficiency, its predictive factors and the association with pregnancy outcomes. Materials and methods This was a prospective observational study in a tertiary teaching hospital over 2-years' duration from August 2013 to August 2015. A total of 680 women who completed 37 weeks of pregnancy were recruited. Results Five hundred and seventy-five patients were enrolled in the final analysis for pregnancy outcomes (response rate of 84...
May 26, 2017: Hormone Molecular Biology and Clinical Investigation
https://www.readbyqxmd.com/read/28583109/prevalence-and-distribution-of-gardnerella-vaginalis-subgroups-in-women-with-and-without-bacterial-vaginosis
#10
Migle Janulaitiene, Virginija Paliulyte, Svitrigaile Grinceviciene, Jolita Zakareviciene, Alma Vladisauskiene, Agne Marcinkute, Milda Pleckaityte
BACKGROUND: Bacterial vaginosis (BV) is one of the leading causes of vaginal complaints among women of childbearing age. The role of Gardnerella vaginalis remains controversial due to its presence in healthy and BV-type vaginal microflora. The phenotypic and genotypic heterogeneity of G. vaginalis suggested the existence of strain variants linked with different health conditions. We sought to analyze prevalence and distribution of G. vaginalis subgroups (clades) in BV-positive (n = 29), partial BV (n = 27), and BV-negative (n = 53) vaginal samples from Lithuanian women...
June 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28580872/effects-of-lactobacillus-rhamnosus-and-lactobacillus-acidophilus-on-bacterial-vaginal-pathogens
#11
Lucia Bertuccini, Rosario Russo, Francesca Iosi, Fabiana Superti
The human vagina is colonized by a variety of microbes. Lactobacilli are the most common, mainly in healthy women; however, the microbiota composition can change rapidly, leading to infection or to a state in which potential pathogenic microorganisms co-exist with other commensals. In premenopausal women, urogenital infections, such as bacterial vaginosis and aerobic vaginitis, remain an important health problem. Treatment of these infections involves different kind of antibiotics; however, the recurrence rate remains high, and it must be also underlined that antibiotics are unable to spontaneously restore normal flora characterized by an abundant community of Lactobacilli...
June 2017: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/28569142/bacterial-vaginosis-human-papilloma-virus-and-herpes-viridae-do-not-predict-vaginal-hiv-rna-shedding-in-women-living-with-hiv-in-denmark
#12
Maria Wessman, Kristina Thorsteinsson, Jørgen S Jensen, Merete Storgaard, Frederikke F Rönsholt, Isik S Johansen, Gitte Pedersen, Lars Nørregård Nielsen, Jesper Bonde, Terese L Katzenstein, Nina Weis, Anne-Mette Lebech
BACKGROUND: Bacterial vaginosis (BV) has been found to be associated with HIV acquisition and transmission. This is suggested to be due to higher HIV RNA levels in cervicovaginal fluids in women living with HIV (WLWH) with BV, as bacteria associated with BV may induce viral replication and shedding in the genital tract despite undetectable HIV RNA plasma viral load. We examined the prevalence and diagnostic predictors of BV and HIV-1 RNA vaginal shedding in women living with HIV (WLWH) in Denmark, taking into account the presence of human papillomavirus (HPV) and herpes viridae...
May 31, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28562623/relationship-between-nugent-score-and-vaginal-epithelial-exfoliation
#13
Courtney P Amegashie, Nicole M Gilbert, Jeffrey F Peipert, Jenifer E Allsworth, Warren G Lewis, Amanda L Lewis
OBJECTIVE: Clue cells characteristic of bacterial vaginosis (BV) are thought to arise due to exfoliation of the vaginal epithelium; however, there is little published data connecting total numbers of epithelial cells to markers of BV. The purpose of this study was to enumerate exfoliated epithelial cells (independent of clue cells) and examine the relationship to Nugent score. STUDY DESIGN: We conducted a cross-sectional sub-study of the Contraceptive CHOICE Project cohort...
2017: PloS One
https://www.readbyqxmd.com/read/28555888/computerized-detection-of-leukocytes-in-microscopic-leukorrhea-images
#14
Jing Zhang, Ya Zhong, Xiangzhou Wang, Guangming Ni, Xiaohui Du, Juanxiu Liu, Liu Lin, Yong Liu
PURPOSE: Detection of leukocytes is critical for the routine leukorrhea exam, which is widely used in gynecological examinations. An elevated vaginal leukocyte count in women with bacterial vaginosis is a strong predictor of vaginal or cervical infections. In the routine leukorrhea exam, the counting of leukocytes is primarily performed by manual techniques. However, the viewing and counting of leukocytes from multiple high-power viewing fields on a glass slide under a microscope leads to subjectivity, low efficiency, and low accuracy...
May 29, 2017: Medical Physics
https://www.readbyqxmd.com/read/28552417/quantification-isolation-and-characterization-of-bifidobacterium-from-the-vaginal-microbiomes-of-reproductive-aged-women
#15
Aline C Freitas, Janet E Hill
The vaginal microbiome plays an important role in women's reproductive health. Imbalances in this microbiota, such as the poorly defined condition of bacterial vaginosis, are associated with increased susceptibility to sexually transmitted infections and negative reproductive outcomes. Currently, a "healthy" vaginal microbiota in reproductive aged women is understood to be dominated by Lactobacillus, although "atypical" microbiomes, such as Bifidobacterium-dominated profiles, have been described. Despite these observations, vaginal bifidobacteria remain relatively poorly characterized, and questions remain regarding their actual abundance in the microbiome...
May 25, 2017: Anaerobe
https://www.readbyqxmd.com/read/28546029/gardnerella-vaginalis-bacteremia-associated-with-severe-acute-encephalopathy-in-a-young-female-patient
#16
Jacques Tankovic, Albertas Timinskas, Migle Janulaitiene, Milda Zilnyte, Jean-Luc Baudel, Eric Maury, Aurelija Zvirbliene, Milda Pleckaityte
Gardnerella vaginalis is a facultative anaerobic bacterium that inhabits the genitourinary tract of both healthy women and those with bacterial vaginosis. We report a case of G. vaginalis bacteremia associated with severe toxic encephalopathy in a young woman. Anaerobic blood cultures yielded pure growth of small gram-variable rods later identified as G. vaginalis by both rapid biochemical tests and 16S rRNA gene sequencing. The patient recovered after treatment with amoxicillin-clavulanate according to the in vitro susceptibility testing...
May 22, 2017: Anaerobe
https://www.readbyqxmd.com/read/28545241/lactobacillus-rhamnosus-hn001-and-lactobacillus-acidophilus-la-14-attenuate-gardnerella-vaginalis-infected-bacterial-vaginosis-in-mice
#17
Se-Eun Jang, Jin-Ju Jeong, Su-Young Choi, Hyunji Kim, Myung Joo Han, Dong-Hyun Kim
Oral administration of a probiotic mixture (PM; Respecta(®)) consisting of Lactobacillus rhamnosus HN001 (L1), Lactobacillus acidophilus La-14 (L2), and lactoferrin RCXTM results in colonization of these probiotics in the vagina of healthy women. Therefore, we examined whether vaginal colonization of the PM ingredients L1 and L2 could attenuate bacterial vaginosis (BV). BV was induced in mice via β-estradiol-3-benzoate-induced immunosuppression and intravaginal inoculation with Gardnerella vaginalis (GV)...
May 23, 2017: Nutrients
https://www.readbyqxmd.com/read/28542929/the-immune-response-to-prevotella-bacteria-in-chronic-inflammatory-disease
#18
REVIEW
Jeppe Madura Larsen
The microbiota plays a central role in human health and disease by shaping immune development, immune responses, metabolism, and protecting from invading pathogens. Technical advances that allow comprehensive characterization of microbial communities by genetic sequencing have sparked the hunt for disease modulating bacteria. Emerging studies in humans have linked increased abundance of Prevotella species at mucosal sites to localized and systemic disease, including periodontitis, bacterial vaginosis, rheumatoid arthritis, metabolic disorders, and low-grade systemic inflammation...
May 19, 2017: Immunology
https://www.readbyqxmd.com/read/28539726/development-and-validation-of-a-simultaneous-rp-hplcuv-dad-method-for-determination-of-polyphenols-in-gels-containing-s-terebinthifolius-raddi-anacardiaceae
#19
Melina G Carvalho, Cícero F S Aragão, Fernanda N Raffin, Túlio F A de L Moura
Topical gels containing extracts of Schinus terebinthifolius have been used to treat bacterial vaginosis. It has been reported that this species has antimicrobial, anti-inflammatory and anti-ulcerogenic properties, which can be attributed to the presence of phenolic compounds. In this work, a sensitive and selective reversed-phase HPLC-UV/DAD method for the simultaneous assay of six polyphenols that could be present in S. terebinthifolius was developed. The method was shown to be accurate and precise. Peak purity and similarity index both exceeded 0...
April 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28531212/epidemiological-investigation-of-the-relationship-between-common-lower-genital-tract-infections-and-high-risk-human-papillomavirus-infections-among-women-in-beijing-china
#20
Dai Zhang, Ting Li, Lei Chen, Xiaosong Zhang, Gengli Zhao, Zhaohui Liu
BACKGROUND: The incidence of lower genital tract infections in China has been increasing in recent years. The link between high-risk human papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) remains unclear. METHODS: From March to October 2014, gynecological examinations and questionnaires were conducted on 1218 married women. Cervical secretions and vaginal swab specimens were tested for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Ureaplasma urealyticum (UU), yeast, clue cells and HR-HPV...
2017: PloS One
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