keyword
MENU ▼
Read by QxMD icon Read
search

MALDI-TOF

keyword
https://www.readbyqxmd.com/read/29158151/a-novel-liquid-media-mycobacteria-extraction-method-for-maldi-tof-ms-identification-using-vitek%C3%A2-ms
#1
Erik Moreno, Eric Miller, Elizabeth Miller, Heather Totty, Parampal Deol
A low-cost identification method that can be performed directly from a positive liquid medium culture is needed for the diagnosis of mycobacterial infections. Here, we describe a novel, cost-effective, and validated method that allows for direct and rapid identification of mycobacteria from a positive liquid culture using VITEK® MS with a total process duration under 45min. From a liquid mycobacteria culture a 3.0mL aliquot is removed 24-72h post positivity and centrifuged to create a pellet. After decanting, the tube is blotted dry, the pellet is re-suspended in 0...
November 17, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29157262/immunoproteomic-analysis-of-the-excretory-secretory-products-of-trichinella-pseudospiralis-adult-worms-and-newborn-larvae
#2
Yang Wang, Xue Bai, Haichao Zhu, Xuelin Wang, Haining Shi, Bin Tang, Pascal Boireau, Xuepeng Cai, Xuenong Luo, Mingyuan Liu, Xiaolei Liu
BACKGROUND: The nematode Trichinella pseudospiralis is an intracellular parasite of mammalian skeletal muscle cells and exists in a non-encapsulated form. Previous studies demonstrated that T. pseudospiralis could induce a lower host inflammatory response. Excretory-secretory (ES) proteins as the most important products of host-parasite interaction may play the main functional role in alleviating host inflammation. However, the ES products of T. pseudospiralis early stage are still unknown...
November 21, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29157222/comparative-proteomics-reveals-the-physiological-differences-between-winter-tender-shoots-and-spring-tender-shoots-of-a-novel-tea-camellia-sinensis-l-cultivar-evergrowing-in-winter
#3
Shengjie Liu, Jiadong Gao, Zhongjian Chen, Xiaoyan Qiao, Hualin Huang, Baiyuan Cui, Qingfeng Zhu, Zhangyan Dai, Hualing Wu, Yayan Pan, Chengwei Yang, Jun Liu
BACKGROUND: A recently discovered tea [Camellia sinensis (L.) O. Kuntze] cultivar can generate tender shoots in winter. We performed comparative proteomics to analyze the differentially accumulated proteins between winter and spring tender shoots of this clonal cultivar to reveal the physiological basis of its evergrowing character during winter. RESULTS: We extracted proteins from the winter and spring tender shoots (newly formed two leaves and a bud) of the evergrowing tea cultivar "Dongcha11" respectively...
November 20, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29156685/whole-genome-and-transcriptome-analysis-reveal-maldi-tof-ms-and-sds-page-have-limited-performance-for-the-detection-of-the-key-outer-membrane-protein-in-carbapenem-resistant-klebsiella-pneumoniae-isolates
#4
Naina Adren Pinto, Roshan D'Souza, In Sik Hwang, Jongrak Choi, Yong Ha In, Hyung Soon Park, Choong-Min Ryu, Dongeun Yong, Kyungwon Lee
To detect the outer membrane protein (OMP), which plays a key role in carbapenem resistance, whole-genome and transcriptome analysis of the clinical carbapenem-resistant Klebsiella pneumoniae was carried out. The index strain lacked both OmpK35 and OmpK36, whereas the other strains lacked only OmpK35. After SDS-PAGE, the putative OMP bands were excised and identified as OmpA and OmpK36. MALDI-TOF MS showed peaks at ∼36 and ∼38 kDa that corresponded to OmpA and OmpK36, respectively. In all the strains except YMC2014/03/P345, the ∼38 kDa peaks were present...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156586/in-depth-glyco-peptidomics-approach-reveals-unexpected-diversity-of-glycosylated-peptides-and-atypical-post-translational-modifications-in-dendroaspis-angusticeps-snake-venom
#5
Michel Degueldre, Julien Echterbille, Nicolas Smargiasso, Christian Damblon, Charlotte Gouin, Gilles Mourier, Nicolas Gilles, Edwin De Pauw, Loïc Quinton
Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs. In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years. It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges. These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors...
November 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29156513/production-and-characterization-of-keratinolytic-proteases-by-a-chicken-feather-degrading-thermophilic-strain-thermoactinomyces-sp-yt06
#6
Lin Wang, Yuting Qian, Yun Cao, Ying Huang, Zhizhou Chang, Hongying Huang
Thermoactinomyces sp. strain YT06 was isolated from poultry compost and observed to degrade integral chicken feathers at 60°C completely, resulting in the formation of 3.24 mg/mL of free amino acid from 50 mL of culture containing 10 g/L chicken feathers. Strain YT06 could grow and secret keratinase using feather as the only carbon and nitrogen sources without other supplement, but complementation of 10 g/L sucrose and 4 g/L NaNO₃ increased the production of the keratinolytic enzyme. The maximum protease activities obtained were 110 U/mL and keratinase were 42 U/mL...
November 21, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29155052/characterization-of-covalent-modifications-of-hdl-apoproteins-by-endogenous-oxidized-phospholipids
#7
Detao Gao, Eugene A Podrez
High density lipoprotein (HDL) is cardioprotective, unless it is pathologically modified under oxidative stress. Covalent modifications of lipid-free apoA-I, the most abundant apoprotein in HDL, compromise its atheroprotective functions. HDL is enriched in oxidized phospholipids (oxPL) in vivo in oxidative stress. Furthermore, oxidized phospholipids can covalently modify HDL apoproteins. We have now carried out a systematic analysis of modifications of HDL apoproteins by endogenous oxPL. Human HDL or plasma were oxidized using a physiologically relevant MPO-H2O2-NO2(-) system or AIPH, or were exposed to synthetic oxPL...
November 15, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29154788/a-multianalytical-approach-to-investigate-the-effect-of-nanofiltration-on-plasma-derived-factor-ix-clinical-lots
#8
Rosita Russo, Giuseppina Focà, Camilla Rega, Annamaria Sandomenico, Nunzianna Doti, Filippo Mori, Marcella Maddaluno, Claudio Farina, Menotti Ruvo, Angela Chambery
Plasma-derived proteins are a subset of relevant biotherapeutics also known as "well-characterized biologicals". They are enriched from plasma through several steps of physical and biochemical methodologies, reaching the regulatory accepted standards of safety, levels of impurities, activity and lot-to-lot consistency. Final products accepted for commercialization are submitted to tight analytical, functional and safety controls by a number of different approaches that fulfill the requirements of sensitivity and reliability...
November 14, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29153517/rapid-species-and-subspecies-specific-level-classification-and-identification-of-lactobacillus-casei-group-members-using-maldi-biotyper-combined-with-clinprotools
#9
Chien-Hsun Huang, Lina Huang
Using common taxonomic methods such as 16S rDNA sequencing and physiological and biochemical analysis to identify members of the Lactobacillus casei group (LCG) is time-consuming, expensive and inaccurate. In this study, we applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapid discriminate LCG strains by creating an analytical in-house database (IHDB) and to develop a classification model for subspecies-level differentiation based on MS biomarkers using ClinProTools bioinformatics software (Bruker Daltonics, Billerica, MA)...
November 15, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29153274/-classical-and-molecular-methods-for-identification-and-quantification-of-domestic-moulds
#10
REVIEW
E Fréalle, V Bex, G Reboux, S Roussel, S Bretagne
INTRODUCTION: To study the impact of the constant and inevitable inhalation of moulds, it is necessary to sample, identify and count the spores. BACKGROUND: Environmental sampling methods can be separated into three categories: surface sampling is easy to perform but non quantitative, air sampling is easy to calibrate but provides time limited information, and dust sampling which is more representative of long term exposure to moulds. The sampling strategy depends on the objectives (evaluation of the risk of exposure for individuals; quantification of the household contamination; evaluation of the efficacy of remediation)...
November 15, 2017: Revue des Maladies Respiratoires
https://www.readbyqxmd.com/read/29151823/proteomic-changes-between-populus-allotriploids-and-diploids-revealed-using-an-itraq-based-quantitative-approach
#11
Yi Wang, Yun Li, Yujing Suo, Yu Min, Xiangyang Kang
Background: Polyploid breeding is a powerful approach for Populus genetic improve-ment because polyploid trees have valuable characteristics, including better timber quality and a higher degree of stress resistance compared with their full-sib diploids. However, the genetic mech-anism underlying this phenomenon remains unknown. Objective: To better understand the proteomic changes between Populus allotriploids and diploids, we examined the proteomic profiles of allotriploid and diploid Populus by iTRAQ labeling coupled with two-dimensional liquid chromatography and MALDI-TOF/TOF mass spectrometry (MS)...
September 2017: Current Proteomics
https://www.readbyqxmd.com/read/29149220/a-novel-method-to-rapidly-distinguish-the-geographical-origin-of-traditional-fermented-salted-vegetables-by-mass-fingerprinting
#12
So-Ra Yoon, Sung Hyun Kim, Hae-Won Lee, Ji-Hyoung Ha
The geographical origin of kimchi is of interest to consumers and producers because the prices of commercial kimchi products can vary significantly according to the geographical origin. Hence, social issues related to the geographical origin of kimchi in Korea have emerged as a major problem. In this study, the geographical origin of kimchi was determined by comparing the mass fingerprints obtained for Korean and Chinese kimchi samples by MALDI-TOF MS with multivariate analysis. The results obtained herein provide an accurate, powerful tool to clearly discriminate kimchi samples based on their geographical origin within a short time and to ensure food authenticity, which is of significance in the kimchi industry...
2017: PloS One
https://www.readbyqxmd.com/read/29148919/bacterial-flora-analysis-of-coliforms-in-sewage-river-water-and-ground-water-using-maldi-tof-mass-spectrometry
#13
Yoshihiro Suzuki, Kouki Niina, Tomonori Matsuwaki, Kei Nukazawa, Atsushi Iguchi
The aim of this study was to rapidly and effectively analyze coliforms, which are the most fundamental indicators of water quality for fecal pollution, using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Coliform bacteria were isolated from municipal sewage, river water, and groundwater. For each sample, 100 isolates were determined by MALDI-TOF MS. In addition, these same 100 isolates were also identified via 16S rRNA gene sequence analysis. Obtained MALDI-TOF MS data were compared with the 16S rRNA sequencing analysis, and the validity of MALDI-TOF MS for classification of coliform bacteria was examined...
November 17, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29148742/identification-of-the-peptide-pyroq-%C3%AE-casein194-209-as-a-highly-specific-and-sensitive-marker-to-differentiate-between-ultrahigh-temperature-processed-uht-milk-and-mildly-heated-milk
#14
Sevim Dalabasmaz, Jennifer Ebner, Monika Pischetsrieder
In this study, a new approach was introduced to identify marker peptides that reflect the thermal treatment of commercial milk samples and differentiate ultrahigh-temperature processed (UHT) milk from mildly heated milk. Peptide profiles of training set samples, pasteurized (n=20), extended shelf life (n=29), and UHT (n=29) milk, were recorded by MALDI-TOF-MS after StageTip micro-extraction. As marker candidates, 13 peptides were selected and their cutoff levels were defined. The quality of the cutoff levels was then tested with a blind test set...
November 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29148176/differential-proteomic-analysis-reveals-protein-networks-and-pathways-that-may-contribute-to-helicobacter-pylori-fkbp-type-ppiase-associated-gastric-diseases
#15
Yanmei Zhu, Yuehua Gong, Aodi Li, Moye Chen, Dan Kang, Jun Liu, Yuan Yuan
PURPOSE: Though Helicobacter pylori (H. pylori) has been classified as class I carcinogen, key virulence factor generated by H. pylori that causes gastric cancer remains to be fully determined. Recently, we identified a gastric cancer-associated H. pylori gene, peptidylprolyl isomerase-FK506 binding protein (PPIase-FKBP), and showed that PPIase-FKBP was capable of inducing oncogenic transformation of gastric epithelial cells. But its mechanism was unclear. EXPERIMENTAL DESIGN: We carried out a comparative proteomic analysis of human gastric epithelial cells that either express PPIase-FKBP or green fluorescent protein (GFP) using two-dimensional (2-D) gel electrophoresis and then matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS/MS)...
November 16, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29146249/composition-of-lactic-acid-bacteria-during-spontaneous-curly-kale-brassica-oleracea-var-sabellica-fermentation
#16
Magdalena Michalak, Klaudia Gustaw, Adam Waśko, Magdalena Polak-Berecka
The present work is the first report on spontaneous fermentation of curly kale and characteristics of autochthonous lactic acid bacteria (LAB). Our results indicate that curly kale fermentation is the new possibility of the technological use of this vegetable. Bacteria representing ten different species were isolated from three phases of curly kale fermentation and identified by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. Among them, four species were identified as Lactobacillus spp. (Lb. plantarum 332, Lb...
January 2018: Microbiological Research
https://www.readbyqxmd.com/read/29143879/comparison-of-analytical-tools-appropriate-for-identification-of-proteinaceous-additives-in-historical-mortars
#17
Iva Krizova, Julia Schultz, Ivan Nemec, Radomir Cabala, Radovan Hynek, Stepanka Kuckova
Natural organic additives such as eggs, lard, resins, and oils have been added to mortars since ancient times, because the ancient builders knew of their positive effect on the mortar quality. The tradition of adding organic materials to mortars was commonly handed down only verbally for thousands years. However, this practice disappeared in the nineteenth century, when the usage of modern materials started. Today, one of the most recent topics in the industry of building materials is the reusing of natural organic materials and searching for the forgotten ancient recipes...
November 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29142047/multi-center-evaluation-of-the-vitek-ms-v3-0-system-for-the-identification-of-filamentous-fungi
#18
Jenna Rychert, E Sue Slechta, Adam P Barker, Edwin Miranda, N Esther Babady, Yi-Wei Tang, Connie Gibas, Nathan Wiederhold, DeAnna Sutton, Kimberly E Hanson
Invasive fungal infections are an important cause of morbidity and mortality affecting primarily immunocompromised patients. While fungal identification to the species level is critical to providing appropriate therapy, it can be slow, laborious, and often relies on subjective morphologic criteria. The use of MALDI-TOF mass spectrometry has the potential to speed up and improve the accuracy of identification. In this multi-center study, we evaluated the accuracy of the VITEK® MS v3.0 system in identifying 1,601 clinical mold isolates as compared to identification by DNA sequence analysis and supported by morphologic and phenotypic testing...
November 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29138856/differentially-expressed-proteins-in-the-human-esophageal-cancer-cell-line-eca%C3%A2-109-in-the-presence-and-absence-of-gemcitabine
#19
Zenghuang Ma, Xiaojie Xue
The present study aimed to screen and study the roles of differentially expressed proteins in the human esophageal cancer cell line Eca‑109, in the presence and absence of gemcitabine (GEM). The 3‑(4,5)‑dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) method was used to assay the vitality of the Eca‑109 cells following treatment with GEM (1‑16 µg/ml). The cell apoptosis was measured by using fluorescence activated cell sorting. The proteins in the treated Eca‑109 cells were extracted, validated, and assayed via two‑dimensional gel electrophoresis combined with matrix‑assisted laser desorption/ionization time of flight mass spectrometry (MALDI‑TOF‑MS)...
November 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29138467/bacillus-classification-based-on-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-effects-of-culture-conditions
#20
Lin-Jie Shu, Yu-Liang Yang
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a reliable and rapid technique applied widely in the identification and classification of microbes. MALDI-TOF MS has been used to identify many endospore-forming Bacillus species; however, endospores affect the identification accuracy when using MALDI-TOF MS because they change the protein composition of samples. Since culture conditions directly influence endospore formation and Bacillus growth, in this study we clarified how culture conditions influence the classification of Bacillus species by using MALDI-TOF MS...
November 14, 2017: Scientific Reports
keyword
keyword
17563
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"