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JOURNAL ARTICLE
Caterina Pipino, Ángel Bernabé-García, Ilaria Cappellacci, Javier Stelling-Férez, Pamela Di Tomo, Manuela Santalucia, Carlos Navalón, Assunta Pandolfi, Francisco José Nicolás
One of the most relevant diabetes complications is impaired wound healing, mainly characterized by reduced peripheral blood flow and diminished neovascularization together with increased inflammation and oxidative stress. Unfortunately, effective therapies are currently lacking. Recently, the amniotic membrane (AM) has shown promising results in wound management. Here, the potential role of AM on endothelial cells isolated from the umbilical cord vein of gestational diabetes-affected women (GD-HUVECs), has been investigated...
2022: Frontiers in Bioengineering and Biotechnology
#22
JOURNAL ARTICLE
Brianna Bradley, Grace Hamilton, Teri Douglas, Anjali Pillai, Robert Fick, Drew Hargrove, Brendan Le, Maitri Unjia, Raymond Trievel, Allison Stelling
SAM (S-adenosyl-L-Methionine) is the universal methyl donor used by cells, and is also the second most used cofactor after ATP. Methyltransferases catalyze the methylation of numerous biomolecules including DNA, protein, and RNA; and have a broad array of functions ranging from cell signaling to gene transcription. Abnormal levels of methyltransferases have been linked to malignant cellular states, cardiovascular diseases, and neurological diseases. Therefore, methyltransferases are an attractive drug target...
May 2022: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
#23
JOURNAL ARTICLE
Janina Linnik, Mohammedyaseen Syedbasha, Yvonne Hollenstein, Jörg Halter, Adrian Egli, Jörg Stelling
To assess the response to vaccination, quantity (concentration) and quality (avidity) of neutralizing antibodies are the most important parameters. Specifically, an increase in avidity indicates germinal center formation, which is required for establishing long-term protection. For influenza, the classical hemagglutination inhibition (HI) assay, however, quantifies a combination of both, and to separately determine avidity requires high experimental effort. We developed from first principles a biophysical model of hemagglutination inhibition to infer IgG antibody avidities from measured HI titers and IgG concentrations...
January 2022: PLoS Pathogens
#24
JOURNAL ARTICLE
Beykin Gala, Stell Laurel, Halim Muhammad Sohail, Nuñez Mariana, Popova Lilia, Nguyen Bac T, Groth Sylvia L, Dennis Amy, Li Zhongqiu, Atkins Melissa, Khavari Tom, Wang Sophia Y, Chang Robert, Fisher Ann C, Sepah Yasir J, Goldberg Jeffrey L
PURPOSE: No approved therapies directly target retinal ganglion cells (RGCs) for neuroprotection or neuroenhancement in glaucoma. Recombinant human nerve growth factor (rhNGF) has been shown to promote RGC survival and function in animal models of optic neuropathy. Here we evaluate safety, tolerability, and efficacy of short-term, high-dose rhNGF eye drops versus placebo in a cohort of glaucoma patients. METHODS: This study is a single-center, randomized, double-masked, vehicle-controlled, parallel group study designed to assess safety and tolerability as well as short-term neuroenhancement of structure and function (Clinicaltrials...
November 12, 2021: American Journal of Ophthalmology
#25
JOURNAL ARTICLE
Abdênego Rodrigues da Silva, Weslley Felix de Oliveira, Pollyanna Michelle da Silva, Leydianne Leite de Siqueira Patriota, Robson Raion de Vasconcelos Alves, Ana Patrícia Silva de Oliveira, Maria Tereza Dos Santos Correia, Patrícia Maria Guedes Paiva, Marilene Henning Vainstein, Paulo Euzébio Cabral Filho, Adriana Fontes, Thiago Henrique Napoleão
This study reports the development of conjugates based on quantum dots (QD)s and lectins from Schinus terebinthifolia leaves (SteLL) and Punica granatum sarcotesta (PgTeL). Cryptococcus neoformans cells were chosen to evaluate the efficiency of the conjugates. Lectins were conjugated to QDs via adsorption, and the optical parameters (emission and absorption) were monitored. Lectin stability in the conjugates towards denaturing agents was investigated via fluorometry. The conjugation was evaluated using fluorescence microplate (FMA) and hemagglutination (HA) assays...
December 1, 2021: International Journal of Biological Macromolecules
#26
JOURNAL ARTICLE
Ke Qiu, Wendu Pang, Jianqing Qiu, Junhong Li, Danni Cheng, Yufang Rao, Yijun Dong, Minzi Mao, Qiurui Liu, Xiaosong Mu, Wei Zhang, Wei Xu, Jianjun Ren, Yu Zhao
BACKGROUND: A universally acknowledged cancer staging system considering all aspects of the T-, N-, and M-classifications for middle ear squamous cell carcinoma (MESCC) remains absent, limiting the clinical management of MESCC patients. MATERIALS AND METHODS: A total of 214 MESCC patients were extracted from the SEER (the Surveillance, Epidemiology, and End Results) database between 1973 and 2016. The relationships between patient's characteristics and prognoses were analyzed by Kaplan-Meier and Cox proportional hazards regression models...
November 2021: Cancer Medicine
#27
MULTICENTER STUDY
Janina Linnik, Mohammedyaseen Syedbasha, Hans-Michael Kaltenbach, Dominik Vogt, Yvonne Hollenstein, Lukas Kaufmann, Nathan Cantoni, Sabine Ruosch-Girsberger, Antonia M S Müller, Urs Schanz, Thomas Pabst, Georg Stüssi, Maja Weisser, Jörg Halter, Jörg Stelling, Adrian Egli
BACKGROUND: Influenza vaccination efficacy is reduced after hematopoietic stem cell transplantation (HSCT) and patient factors determining vaccination outcomes are still poorly understood. METHODS: We investigated the antibody response to seasonal influenza vaccination in 135 HSCT patients and 69 healthy volunteers (HVs) in a prospective observational multicenter cohort study. We identified patient factors associated with hemagglutination inhibition titers against A/California/2009/H1N1, A/Texas/2012/H3N2, and B/Massachusetts/2012 by multivariable regression on the observed titer levels and on seroconversion/seroprotection categories for comparison...
April 19, 2022: Journal of Infectious Diseases
#28
JOURNAL ARTICLE
Mariana Paranhos Stelling, Mariana Alves Soares, Simone Coutinho Cardoso, Juliana Maria Motta, Joice Côrtes de Abreu, Maria Júlia Mansur Antunes, Vitória Gonçalves de Freitas, João Alfredo Moraes, Morgana Teixeira Lima Castelo-Branco, Carlos Alberto Pérez, Mauro Sérgio Gonçalves Pavão
Metastatic disease remains the leading cause of death in cancer and understanding the mechanisms involved in tumor progression continues to be challenging. This work investigates the role of manganese in tumor progression in an in vivo model of tumor growth. Our data revealed that manganese accumulates within primary tumors and secondary organs as manganese-rich niches. Consequences of such phenomenon were investigated, and we verified that short-term changes in manganese alter cell surface molecules syndecan-1 and β1-integrin, enhance collective cell migration and invasive behavior...
August 4, 2021: Scientific Reports
#29
JOURNAL ARTICLE
Melanie Ricke-Hoch, Elisabeth Stelling, Lisa Lasswitz, Antonia P Gunesch, Martina Kasten, Francisco J Zapatero-Belinchón, Graham Brogden, Gisa Gerold, Thomas Pietschmann, Virginie Montiel, Jean-Luc Balligand, Federica Facciotti, Emilio Hirsch, Thomas Gausepohl, Husni Elbahesh, Guus F Rimmelzwaan, Anne Höfer, Mark P Kühnel, Danny Jonigk, Julian Eigendorf, Uwe Tegtbur, Lena Mink, Michaela Scherr, Thomas Illig, Axel Schambach, Tobias J Pfeffer, Andres Hilfiker, Axel Haverich, Denise Hilfiker-Kleiner
The SARS-CoV-2 coronavirus has led to a pandemic with millions of people affected. The present study finds that risk-factors for severe COVID-19 disease courses, i.e. male sex, older age and sedentary life style are associated with higher prostaglandin E2 (PGE2) serum levels in blood samples from unaffected subjects. In COVID-19 patients, PGE2 blood levels are markedly elevated and correlate positively with disease severity. SARS-CoV-2 induces PGE2 generation and secretion in infected lung epithelial cells by upregulating cyclo-oxygenase (COX)-2 and reducing the PG-degrading enzyme 15-hydroxyprostaglandin-dehydrogenase...
2021: PloS One
#30
JOURNAL ARTICLE
Robert J Fick, Amy Y Liu, Felix Nussbaumer, Christoph Kreutz, Atul Rangadurai, Yu Xu, Roger D Sommer, Honglue Shi, Steve Scheiner, Allison L Stelling
Measuring the strength of the hydrogen bonds between DNA base pairs is of vital importance for understanding how our genetic code is physically accessed and recognized in cells, particularly during replication and transcription. Therefore, it is important to develop probes for these key hydrogen bonds (H-bonds) that dictate events critical to cellular function, such as the localized melting of DNA. The vibrations of carbonyl bonds are well-known probes of their H-bonding environment, and their signals can be observed with infrared (IR) spectroscopy...
July 8, 2021: Journal of Physical Chemistry. B
#31
JOURNAL ARTICLE
Nader Tanideh, Giti Borzooeian, Mehrzad Lotfi, Mahsa Sani, Cambyz Irajie, Parvin Ghaemmaghami, Omid Koohi-Hosseinabadi, Romina Tanideh, Mahdi Hashempour Sadeghian, Zahra Borzooeian, Aida Iraji
Osteoarthritis (OA) is an inflammatory joint condition, still lacking effective treatments. Some factors consider as the main causes of OA, including biochemical, mechanical, and genetic factors. The growth of studies confirmed that modern medicine in combination with folk medicine regarding the arrival of reliable, efficient, and safe therapeutic products against OA. In the present study, the effects of various single and combinatorial treatments of knee articular cartilage, including stem cells, collagen, and P...
November 2021: Artificial Organs
#32
JOURNAL ARTICLE
Talita Stelling de Araujo, Glauce Moreno Barbosa, Karoline Sanches, Jéssica M Azevedo, Katia Maria Dos Santos Cabral, Marcius S Almeida, Fabio C L Almeida
During the past 17 years, the coronaviruses have become a global public emergency, with the first appearance in 2012 in Saudi Arabia of the Middle East respiratory syndrome. Among the structural proteins encoded in the viral genome, the nucleocapsid protein is the most abundant in infected cells. It is a multifunctional phosphoprotein involved in the capsid formation, in the modulation and regulation of the viral life cycle. The N-terminal domain of N protein specifically interacts with transcriptional regulatory sequence (TRS) and is involved in the discontinuous transcription through the melting activity of double-stranded TRS (dsTRS)...
April 29, 2021: Biomolecular NMR Assignments
#33
JOURNAL ARTICLE
Claude Lormeau, Fabian Rudolf, Jörg Stelling
Cells can encode information about their environment by modulating signaling dynamics and responding accordingly. Yet, the mechanisms cells use to decode these dynamics remain unknown when cells respond exclusively to transient signals. Here, we approach design principles underlying such decoding by rationally engineering a synthetic short-pulse decoder in budding yeast. A computational method for rapid prototyping, TopoDesign, allowed us to explore 4122 possible circuit architectures, design targeted experiments, and then rationally select a single circuit for implementation...
March 25, 2021: Nature Communications
#34
JOURNAL ARTICLE
Elisabeth Stelling, Melanie Ricke-Hoch, Sergej Erschow, Steve Hoffmann, Anke Katharina Bergmann, Maren Heimerl, Stefan Pietzsch, Karin Battmer, Alexandra Haase, Britta Stapel, Michaela Scherr, Jean-Luc Balligand, Ofer Binah, Denise Hilfiker-Kleiner
Cardiac levels of the signal transducer and activator of transcription factor-3 (STAT3) decline with age, and male but not female mice with a cardiomyocyte-specific STAT3 deficiency conditional knockout (CKO) display premature age-related heart failure associated with reduced cardiac capillary density. In the present study, isolated male and female CKO-cardiomyocytes exhibit increased prostaglandin (PG)-generating cyclooxygenase-2 (COX-2) expression. The PG-degrading hydroxyprostaglandin-dehydrogenase-15 (HPGD) expression is only reduced in male cardiomyocytes, which is associated with increased prostaglandin D2 (PGD2) secretion from isolated male but not female CKO-cardiomyocytes...
December 2020: PLoS Biology
#35
JOURNAL ARTICLE
Andrew D Yale, Balazs Szladovits, Anneliese J Stell, Scott D Fitzgerald, Simon L Priestnall, Alejandro Suarez-Bonnet
An 8-year-old neutered male French Bulldog was presented with a 2-day history of intermittent vomiting, reduced appetite and recent rapid development of multiple cutaneous masses over the head and neck regions. On presentation, the patient had a moderate volume of pericardial and bilateral pleural effusion. Echocardiography demonstrated irregular, heterogeneous thickening of the walls of the right ventricle and right atrium, consistent with infiltrative intramyocardial disease. Cytological examination of fine needle aspirates from one of the cutaneous masses confirmed a mast cell tumour...
October 2020: Journal of Comparative Pathology
#36
JOURNAL ARTICLE
Mireille M J P E Sthijns, Marlon J Jetten, Sami G Mohammed, Sandra M H Claessen, Rick H W de Vries, Adam Stell, Denise F A de Bont, Marten A Engelse, Didem Mumcuoglu, Clemens A van Blitterswijk, Patricia Y W Dankers, Eelco J P de Koning, Aart A van Apeldoorn, Vanessa L S LaPointe
The clinical success rate of islet transplantation, namely independence from insulin injections, is limited by factors that lead to graft failure, including inflammation, acute ischemia, acute phase response, and insufficient vascularization. The ischemia and insufficient vascularization both lead to high levels of oxidative stress, which are further aggravated by islet encapsulation, inflammation, and undesirable cell-biomaterial interactions. To identify biomaterials that would not further increase damaging oxidative stress levels and that are also suitable for manufacturing a beta cell encapsulation device, we studied five clinically approved polymers for their effect on oxidative stress and islet (alpha and beta cell) function...
January 2021: Biomaterials
#37
REVIEW
Sarah M Keating, Dagmar Waltemath, Matthias König, Fengkai Zhang, Andreas Dräger, Claudine Chaouiya, Frank T Bergmann, Andrew Finney, Colin S Gillespie, Tomáš Helikar, Stefan Hoops, Rahuman S Malik-Sheriff, Stuart L Moodie, Ion I Moraru, Chris J Myers, Aurélien Naldi, Brett G Olivier, Sven Sahle, James C Schaff, Lucian P Smith, Maciej J Swat, Denis Thieffry, Leandro Watanabe, Darren J Wilkinson, Michael L Blinov, Kimberly Begley, James R Faeder, Harold F Gómez, Thomas M Hamm, Yuichiro Inagaki, Wolfram Liebermeister, Allyson L Lister, Daniel Lucio, Eric Mjolsness, Carole J Proctor, Karthik Raman, Nicolas Rodriguez, Clifford A Shaffer, Bruce E Shapiro, Joerg Stelling, Neil Swainston, Naoki Tanimura, John Wagner, Martin Meier-Schellersheim, Herbert M Sauro, Bernhard Palsson, Hamid Bolouri, Hiroaki Kitano, Akira Funahashi, Henning Hermjakob, John C Doyle, Michael Hucka
Systems biology has experienced dramatic growth in the number, size, and complexity of computational models. To reproduce simulation results and reuse models, researchers must exchange unambiguous model descriptions. We review the latest edition of the Systems Biology Markup Language (SBML), a format designed for this purpose. A community of modelers and software authors developed SBML Level 3 over the past decade. Its modular form consists of a core suited to representing reaction-based models and packages that extend the core with features suited to other model types including constraint-based models, reaction-diffusion models, logical network models, and rule-based models...
August 2020: Molecular Systems Biology
#38
JOURNAL ARTICLE
Antinociceptive activity of Schinus terebinthifolia leaf lectin (SteLL) in sarcoma 180-bearing mice.
Dalila de Brito Marques Ramos, Maria Taís de Moura Fontes Araújo, Tarcísio Cícero de Lima Araújo, Yasmym Araújo Silva, Angela Carolina Lima Amorim Dos Santos, Mariana Gama E Silva, Patrícia Maria Guedes Paiva, Rosemairy Luciane Mendes, Thiago Henrique Napoleão
ETHNOPHARMACOLOGY RELEVANCE: Schinus terebinthifolia Raddi leaves have been used in folk medicine due to several properties, including antitumor and analgesic effects. The variable efficacy and adverse effects of analgesic drugs have motivated the search for novel antinociceptive agents. It has been reported that the S. terebinthifolia leaf lectin (SteLL) has antitumor activity against sarcoma 180 in mice. AIM OF THE STUDY: This work aimed to evaluate whether SteLL would reduce cancer pain using an orthotopic tumor model...
September 15, 2020: Journal of Ethnopharmacology
#39
JOURNAL ARTICLE
Yan Yan, Xiao Zhou, Zhongdi Chu, Laurel Stell, Mohammad Ali Shariati, Ruikang K Wang, Yaping Joyce Liao
PURPOSE: To determine the key optical coherence tomography (OCT) and OCT angiography (OCTA) parameters that correlate with visual field loss in optic disc drusen (ODD). DESIGN: Retrospective cross-sectional study. METHODS: Single academic center. Seventeen patients with ODD (29 eyes) and 35 age-matched controls (53 eyes). Static perimetry, OCT, and OCTA imaging of optic disc and macula. Static perimetry, OCT, and OCTA measurements. RESULTS: We investigated the relationship between static perimetry and 14 OCT/OCTA measurements in patients with ODD vs age-matched controls and found 5 key measurements that most correlated with visual field loss included: peripapillary retinal nerve fiber layer (RNFL), macular ganglion cell complex (GCC), peripapillary vessel area density (VAD), macular vessel diameter (VD), and flux...
October 2020: American Journal of Ophthalmology
#40
JOURNAL ARTICLE
Qing Shi, Michelle M Teves, Aisha Lillywhite, Eden B Pagtalunan, William K Stell
Adaptation to changes in ambient light intensity, in retinal cells and circuits, optimizes visual functions. In the retina, light-adaptation results in changes in light-sensitivity and spatiotemporal tuning of ganglion cells. Under light-adapted conditions, contrast sensitivity (CS) of ganglion cells is a bandpass function of spatial frequency; in contrast, dark-adaptation reduces CS, especially at higher spatial frequencies. In this work, we aimed to understand intrinsic neuromodulatory mechanisms that underlie retinal adaptation to changes in ambient light level...
June 2020: Experimental Eye Research
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