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Stell cell

Ross P Wilkie, Andrew R Neal, Craig A Johnston, Nicholas Voute, Christopher S Lancefield, Matthew D Stell, Federico Medda, Edward F Makiyi, Emma M Turner, O Stephen Ojo, Alexandra M Z Slawin, Tomas Lebl, Peter Mullen, David J Harrison, Chris M Ireland, Nicholas J Westwood
The synthesis of dehaloperophoramidine, a non-halogenated derivative of the marine natural product perophoramidine, and its biological activity towards HCT116, HT29 and LoVo colorectal carcinoma cells is reported. A [3,3]-Claisen rearrangement and an epoxide opening/allylsilylation reaction installed the contiguous all-carbon quaternary stereocentres with the required relative stereochemistry.
September 14, 2016: Chemical Communications: Chem Comm
Stell Patadji, Joshua Pantanowitz, Natalie M Perlov, Sara E Monaco
INTRODUCTION: Arborizing stromal meshwork fragments (ASMFs) have been proposed as a useful clue to differentiate mucin associated with mucinous adenocarcinoma from contaminating mucus during endobronchial ultrasound-guided transbronchial needle aspiration. Our aim was to retrospectively review cytology cases with mucinous material to determine the utility of ASMFs in diagnosing mucinous tumors. MATERIAL AND METHODS: Diff-Quik stained smears from archival cytology cases (N = 40) were reviewed, including adenocarcinomas with mucinous features, cystic mucinous neoplasms, and control cases with mucin contamination...
October 2016: Diagnostic Cytopathology
Malte B Monin, Petra Krause, Robin Stelling, Derya Bocuk, Sabine Niebert, Florian Klemm, Tobias Pukrop, Sarah Koenig
BACKGROUND: Wnt/β-catenin signaling is known to play an important role in colorectal cancer (CRC). Niclosamide, a salicylamide derivative used in the treatment of tapeworm infections, targets the Wnt/β-catenin pathway. The objective of this study was to investigate niclosamide as a therapeutic agent against CRC. METHODS: The antiproliferative effects of 1, 3, 10, and 50 μM concentrations of niclosamide on human (SW480 and SW620) and rodent (CC531) CRC cell lines were determined by MTS assay and direct cell count...
June 1, 2016: Journal of Surgical Research
Robert Gnügge, Lekshmi Dharmarajan, Moritz Lang, Jörg Stelling
Feedback loops in biological networks, among others, enable differentiation and cell cycle progression, and increase robustness in signal transduction. In natural networks, feedback loops are often complex and intertwined, making it challenging to identify which loops are mainly responsible for an observed behavior. However, minimal synthetic replicas could allow for such identification. Here, we engineered a synthetic permease-inducer-repressor system in Saccharomyces cerevisiae to analyze if a transport-mediated positive feedback loop could be a core mechanism for the switch-like behavior in the regulation of metabolic gene networks such as the S...
October 21, 2016: ACS Synthetic Biology
Dinesh Pradhan, Zaibo Li, Rebecca Ocque, Stell Patadji, Chengquan Zhao
BACKGROUND: The interpretation of atypical glandular cells (AGC) in Papanicolaou (Pap) tests and screening for glandular neoplasia remain challenging. METHODS: Pap tests with an AGC interpretation from January 2008 to December 2013 were retrieved from a pathology database, and cases with histologic follow-up within 1 year were included in the study. RESULTS: There were 589,830 Pap tests during the study period, and 3709 cases (0.6%) were reported as AGC, which included the following: AGC, not otherwise specified (52...
August 2016: Cancer Cytopathology
Mariana A Soares, Felipe C O B Teixeira, Miguel Fontes, Ana Lúcia Arêas, Marcelo G Leal, Mauro S G Pavão, Mariana P Stelling
The metastatic disease is one of the main consequences of tumor progression, being responsible for most cancer-related deaths worldwide. This review intends to present and discuss data on the relationship between integrins and heparan sulfate proteoglycans in health and cancer progression. Integrins are a family of cell surface transmembrane receptors, responsible for cell-matrix and cell-cell adhesion. Integrins' main functions include cell adhesion, migration, and survival. Heparan sulfate proteoglycans (HSPGs) are cell surface molecules that play important roles as cell receptors, cofactors, and overall direct or indirect contributors to cell organization...
2015: BioMed Research International
Maria Mellinas-Gomez, Victoria J Spanswick, Solange R Paredes-Moscosso, Matthew Robson, R Barbara Pedley, David E Thurston, Stephen J Baines, Anneliese Stell, John A Hartley
BACKGROUND: Cancer is the leading cause of death in older dogs and its prevalence is increasing. There is clearly a need to develop more effective anti-cancer drugs in dogs. SG2000 (SJG-136) is a sequence selective DNA minor groove cross-linking agent. Based on its in vitro potency, the spectrum of in vivo and clinical activity against human tumours, and its tolerability in human patients, SG2000 has potential as a novel therapeutic against spontaneously occurring canine malignancies...
2015: BMC Veterinary Research
Ajeet Kumar Gandhi, Soumyajit Roy, Ahitagni Biswas, Mohd Waseem Raza, Tripti Saxena, Suman Bhasker, Atul Sharma, Alok Thakar, Bidhu Kalyan Mohanti
OBJECTIVE: Carcinoma of external auditory canal (EAC) is a rare disease with variable management strategies and prognosis. We aimed to analyze treatment modalities, prognostic factors and survival outcomes in patients of squamous cell carcinoma of EAC treated at our institution. METHODS: Forty-three patients of squamous cell carcinoma of EAC were analyzed for clinical presentation, stage, surgical procedures and radiotherapy (RT) modalities employed. Stell and McCormick staging system was used for staging of the patients...
February 2016: Auris, Nasus, Larynx
Ana Paula Oliveira, Sotiris Dimopoulos, Alberto Giovanni Busetto, Stefan Christen, Reinhard Dechant, Laura Falter, Morteza Haghir Chehreghani, Szymon Jozefczuk, Christina Ludwig, Florian Rudroff, Juliane Caroline Schulz, Asier González, Alexandre Soulard, Daniele Stracka, Ruedi Aebersold, Joachim M Buhmann, Michael N Hall, Matthias Peter, Uwe Sauer, Jörg Stelling
Cells react to nutritional cues in changing environments via the integrated action of signaling, transcriptional, and metabolic networks. Mechanistic insight into signaling processes is often complicated because ubiquitous feedback loops obscure causal relationships. Consequently, the endogenous inputs of many nutrient signaling pathways remain unknown. Recent advances for system-wide experimental data generation have facilitated the quantification of signaling systems, but the integration of multi-level dynamic data remains challenging...
April 2015: Molecular Systems Biology
D R Killick, A J Stell, B Catchpole
Over the last 50 years, the significance of the immune system in the development and control of cancer has been much debated. However, recent discoveries provide evidence for a role of immunological mechanisms in the detection and destruction of cancer cells. Forty years ago veterinary oncologists were already investigating the feasibility of treating neoplasia by enhancing anticancer immunity. Unfortunately, this research was hindered by lack of a detailed understanding of cancer immunology, this limited the specificity and success of these early approaches...
April 2015: Journal of Small Animal Practice
Adrian Egli, Atul Humar, Lukas A Widmer, Luiz F Lisboa, Deanna M Santer, Thomas Mueller, Joerg Stelling, Aliyah Baluch, Daire O'Shea, Michael Houghton, Deepali Kumar
BACKGROUND: Influenza vaccine immunogenicity is suboptimal in immunocompromised patients. However, there are limited data on the interplay of T- and B- cell responses to vaccination with simultaneous immunosuppression. METHODS: We collected peripheral blood mononuclear cells from transplant recipients before and 1 month after seasonal influenza vaccination. Before and after vaccination, H1N1-specific T- and B-cell activation were quantified with flow cytometry. We also developed a mathematical model using T- and B-cell markers and mycophenolate mofetil (MMF) dosage...
July 1, 2015: Journal of Infectious Diseases
Sebastian M Goerke, Julia Obermeyer, Julia Plaha, G Björn Stark, Günter Finkenzeller
Neovascularization is crucial for fracture healing and plays an important role in long-time graft survival in tissue engineering applications. Endothelial progenitor cells (EPCs) can be isolated from peripheral blood avoiding donor site morbidity, which makes them attractive for autologous cell-based engineering of neovessels. However, contradictory results are published concerning the vasculogenic potential of this cell type. We could previously show that implanted human endothelial vein cells (HUVECs) gave rise to the formation of a complex functional human neovasculature in a heterotopic (subcutaneous) as well as in an orthotopic (calvarial defect) model of severe combined immunodeficiency (SCID) mice, where vessel formation could even be increased by coimplanting mesenchymal stem cells (MSCs) functioning as perivascular cells...
March 2015: Microvascular Research
Dammy Pinheiro, Yu-Mei Chang, Hannah Bryant, Balazs Szladovits, Tim Dalessandri, Lucy J Davison, Elizabeth Yallop, Emily Mills, Chiara Leo, Ana Lara, Anneliese Stell, Gerry Polton, Oliver A Garden
The cancer microenvironment plays a pivotal role in oncogenesis, containing a number of regulatory cells that attenuate the anti-neoplastic immune response. While the negative prognostic impact of regulatory T cells (Tregs) in the context of most solid tissue tumors is well established, their role in lymphoid malignancies remains unclear. T cells expressing FOXP3 and Helios were documented in the fine needle aspirates of affected lymph nodes of dogs with spontaneous multicentric B cell lymphoma (BCL), proposed to be a model for human non-Hodgkin lymphoma...
2014: PloS One
David Ausländer, Simon Ausländer, Ghislaine Charpin-El Hamri, Ferdinand Sedlmayer, Marius Müller, Olivier Frey, Andreas Hierlemann, Jörg Stelling, Martin Fussenegger
All metabolic activities operate within a narrow pH range that is controlled by the CO2-bicarbonate buffering system. We hypothesized that pH could serve as surrogate signal to monitor and respond to the physiological state. By functionally rewiring the human proton-activated cell-surface receptor TDAG8 to chimeric promoters, we created a synthetic signaling cascade that precisely monitors extracellular pH within the physiological range. The synthetic pH sensor could be adjusted by organic acids as well as gaseous CO2 that shifts the CO2-bicarbonate balance toward hydrogen ions...
August 7, 2014: Molecular Cell
Anneliese Fortuna-Costa, Angélica M Gomes, Eliene O Kozlowski, Mariana P Stelling, Mauro S G Pavão
Galectin-3, the only chimera galectin found in vertebrates, is one of the best-studied galectins. It is expressed in several cell types and is involved in a broad range of physiological and pathological processes, such as cell adhesion, cell activation and chemoattraction, cell cycle, apoptosis, and cell growth and differentiation. However, this molecule raises special interest due to its role in regulating cancer cell activities. Galectin-3 has high affinity for β-1,6-N-acetylglucosamine branched glycans, which are formed by the action of the β1,6-N-acetylglucosaminyltransferase V (Mgat5)...
2014: Frontiers in Oncology
Sotiris Dimopoulos, Christian E Mayer, Fabian Rudolf, Joerg Stelling
MOTIVATION: Identifying cells in an image (cell segmentation) is essential for quantitative single-cell biology via optical microscopy. Although a plethora of segmentation methods exists, accurate segmentation is challenging and usually requires problem-specific tailoring of algorithms. In addition, most current segmentation algorithms rely on a few basic approaches that use the gradient field of the image to detect cell boundaries. However, many microscopy protocols can generate images with characteristic intensity profiles at the cell membrane...
September 15, 2014: Bioinformatics
Teresa Cristina Fernandes dos Santos, Angélica Maciel Gomes, Marcos Eduardo Machado Paschoal, Mariana Paranhos Stelling, Vivian Mary Barral Dodd Rumjanek, Alyson do Rosário Junior, Paulo Marcos Valiante, Kalil Madi, Heitor Siffert Pereira de Souza, Mauro Sergio Gonçalves Pavão, Morgana Teixeira Lima Castelo-Branco
BACKGROUND: Heparanase is the only known mammalian glycosidase capable of cleaving heparan sulfate chains. The expression of this enzyme has been associated with tumor development because of its ability to degrade extracellular matrix and promote cell invasion. METHODS: We analyzed heparanase expression in lung cancer samples to understand lung tumor progression and malignancy. Of the samples from 37 patients, there were 14 adenocarcinomas, 13 squamous cell carcinomas, 5 large cell carcinomas, and 5 small cell carcinomas...
August 2014: Biochimica et Biophysica Acta
Kaori Muguruma, William K Stell, Naoyuki Yamamoto
Retinal ganglion cells (GCs) in the Japanese catshark Scyliorhinus torazame were labeled retrogradely with biotinylated dextran amine (BDA3000). First the labeled cells were classified into 5 morphological types (types I-III: small GCs; types IV and V: large GCs) according to the size of the soma and the dendritic arborization pattern as seen in retinal wholemounts. Type I cells were stellate, with dendrites radiating in different directions. Type II cells had bipolar dendritic trees, with 2 primary dendrites extending in opposite directions...
2014: Brain, Behavior and Evolution
Peng Zhang, Wenliang Wang
OBJECTIVE: To prepare the silk fibroin (SF)-chitosan (CS) scaffolds by adjusting the mass ratio between CS and SF, and test and compare the properties of the scaffolds at different mass ratios. METHODS: According to the mass ratios of 6:4 (group A), 6:8 (group B), and 6:16 (group C) between SF and CS, CS-SF scaffolds were prepared by freeze-drying method, respectively. The material properties, porosity, the dissolubility in hot water, the modulus elasticity, and the water absorption expansion rate were measured; the aperture size and shape of scaffolds were observed by scanning electron microscope (SEM)...
December 2013: Chinese Journal of Reparative and Reconstructive Surgery
Bruna da Silveira Paulsen, Simone Coutinho Cardoso, Mariana Paranhos Stelling, Daniel Veloso Cadilhe, Stevens Kastrup Rehen
Schizophrenia has been considered a devastating clinical syndrome rather than a single disease. Nevertheless, the mechanisms behind the onset of schizophrenia have been only partially elucidated. Several studies propose that levels of trace elements are abnormal in schizophrenia; however, conflicting data generated from different biological sources prevent conclusions being drawn. In this work, we used synchrotron radiation X-ray microfluorescence spectroscopy to compare trace element levels in neural progenitor cells (NPCs) derived from two clones of induced pluripotent stem cell lines of a clozapine-resistant schizophrenic patient and two controls...
April 2014: Schizophrenia Research
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