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Saeed Mohammadi, Mohsen Nikbakht, Seyed Mehdi Sajjadi, Fariba Rad, Bahram Chahardouli, Javid Sabour Takanlu, Shahrbano Rostami, Kamran Alimoghaddam, Ardeshir Ghavamzadeh, Seyed H Ghaffari
A predominant challenge in developing curative leukemia therapy is interactions of leukemic cells with the bone marrow stromal microenvironment. We aimed to investigate the role of stromal cells, such as bone marrow mesenchymal stromal cells (BMSCs) and osteoblasts (OBs), in curcumin (CUR) and daunorubicin (DNR) induced apoptosis of acute myeloid leukemia (AML) cells. We used KG1 and U937 as leukemia cell line models and treated them with CUR and DNR. The cells were then co-cultured with BMSCs or a combination of BMSCs and OBs as feeders...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
Irene Otero-Muras, Pencho Yordanov, Joerg Stelling
Bistability has important implications in signaling pathways, since it indicates a potential cell decision between alternative outcomes. We present two approaches developed in the framework of the Chemical Reaction Network Theory for easy and efficient search of multiple steady state behavior in signaling networks (both with and without mass conservation), and apply them to search for sources of bistability at different levels of the interferon signaling pathway. Different type I interferon subtypes and/or doses are known to elicit differential bioactivities (ranging from antiviral, antiproliferative to immunomodulatory activities)...
April 2017: PLoS Computational Biology
Vitalii V Mutsenko, Oleksandr Gryshkov, Lothar Lauterboeck, Olena Rogulska, Dmitriy N Tarusin, Vasilii V Bazhenov, Kathleen Schütz, Sophie Brüggemeier, Elke Gossla, Ashwini R Akkineni, Heike Meißner, Anja Lode, Stephan Meshke, Jane Fromont, Allison L Stelling, Konstantin R Tabachnik, Michael Gelinsky, Sergey Nikulin, Sergey Rodin, Alexander G Tonevitsky, Alexander Y Petrenko, Birgit Glasmacher, Peter J Schupp, Hermann Ehrlich
The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce "ready-to-use" tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol...
March 29, 2017: International Journal of Biological Macromolecules
Vitalii V Mutsenko, Vasilii V Bazhenov, Olena Rogulska, Dmitriy N Tarusin, Kathleen Schütz, Sophie Brüggemeier, Elke Gossla, Ashwini R Akkineni, Heike Meißner, Anja Lode, Stephan Meshke, Andre Ehrlich, Slavica Petović, Rajko Martinovic, Mirko Djurović, Allison L Stelling, Sergey Nikulin, Sergey Rodin, Alexander Tonevitsky, Michael Gelinsky, Alexander Y Petrenko, Birgit Glasmacher, Hermann Ehrlich
The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers...
March 24, 2017: International Journal of Biological Macromolecules
Christian Stelling, Chetan R Singh, Matthias Karg, Tobias A F König, Mukundan Thelakkat, Markus Retsch
In this contribution, the optical losses and gains attributed to periodic nanohole array electrodes in polymer solar cells are systematically studied. For this, thin gold nanomeshes with hexagonally ordered holes and periodicities (P) ranging from 202 nm to 2560 nm are prepared by colloidal lithography. In combination with two different active layer materials (P3HT:PC61BM and PTB7:PC71BM), the optical properties are correlated with the power conversion efficiency (PCE) of the solar cells. A cavity mode is identified at the absorption edge of the active layer material...
February 15, 2017: Scientific Reports
Rafaela C Sartore, Simone C Cardoso, Yury V M Lages, Julia M Paraguassu, Mariana P Stelling, Rodrigo F Madeiro da Costa, Marilia Z Guimaraes, Carlos A Pérez, Stevens K Rehen
Systematic studies of micronutrients during brain formation are hindered by restrictions to animal models and adult post-mortem tissues. Recently, advances in stem cell biology have enabled recapitulation of the early stages of human telencephalon development in vitro. In the present work, we analyzed cerebral organoids derived from human pluripotent stem cells by synchrotron radiation X-ray fluorescence in order to measure biologically valuable micronutrients incorporated and distributed into the exogenously developing brain...
2017: PeerJ
Stell Patadji, Zaibo Li, Dinesh Pradhan, Chengquan Zhao
BACKGROUND: The role of high-risk human papillomavirus (hrHPV) testing in women with various types of atypical glandular cells (AGC) on Papanicolaou (Pap) test is undetermined. METHODS: Herein, the authors retrospectively searched their pathology database for cases of AGC with concurrent hrHPV testing and histological follow-up results within 1 year between January 2008 and December 2013. RESULTS: Among 3709 AGC cases occurring during the study period, 2287 had concurrent Hybrid Capture 2 HPV testing results...
March 2017: Cancer
Jorge E Ramirez, Brandon M Stell
The brain's control of movement is thought to involve coordinated activity between cerebellar Purkinje cells. The results reported here demonstrate that somatic Ca(2+) imaging is a faithful reporter of Na(+)-dependent "simple spike" pauses and enables us to optically record changes in firing rates in populations of Purkinje cells in brain slices and in vivo. This simultaneous calcium imaging of populations of Purkinje cells reveals a striking spatial organization of pauses in Purkinje cell activity between neighboring cells...
December 20, 2016: Cell Reports
Mingqi Xie, Haifeng Ye, Hui Wang, Ghislaine Charpin-El Hamri, Claude Lormeau, Pratik Saxena, Jörg Stelling, Martin Fussenegger
Chronically deregulated blood-glucose concentrations in diabetes mellitus result from a loss of pancreatic insulin-producing β cells (type 1 diabetes, T1D) or from impaired insulin sensitivity of body cells and glucose-stimulated insulin release (type 2 diabetes, T2D). Here, we show that therapeutically applicable β-cell-mimetic designer cells can be established by minimal engineering of human cells. We achieved glucose responsiveness by a synthetic circuit that couples glycolysis-mediated calcium entry to an excitation-transcription system controlling therapeutic transgene expression...
December 9, 2016: Science
Edris Hoseinzadeh, Pouran Makhdoumi, Parisa Taha, John Stelling, Hooshyar Hossini, Mohammad Amjad Kamal, Ghulam Md Ashraf
Nanotechnology is a scientific and engineering technology conducted at the Nano-scale, such as in the fields of compound fabric manufacturing, food processing, agricultural processing, and engineering, as well as in medical and medicinal application. In recent decade, nanomaterial applications for antimicrobial works have been interested by many researchers. Available reports show that some of the metal oxide Nanoparticles including; Al2O3, TiO2, ZnO, CuO, Co3O4, In2O3, MgO, SiO2, ZrO2, Cr2O3, Ni2O3, Mn2O3, CoO, and Nickel oxide have toxicity toward several microorganisms and they could successfully kill numerous bacteria...
December 1, 2016: Current Drug Metabolism
Ross P Wilkie, Andrew R Neal, Craig A Johnston, Nicholas Voute, Christopher S Lancefield, Matthew D Stell, Federico Medda, Edward F Makiyi, Emma M Turner, O Stephen Ojo, Alexandra M Z Slawin, Tomas Lebl, Peter Mullen, David J Harrison, Chris M Ireland, Nicholas J Westwood
The synthesis of dehaloperophoramidine, a non-halogenated derivative of the marine natural product perophoramidine, and its biological activity towards HCT116, HT29 and LoVo colorectal carcinoma cells is reported. A [3,3]-Claisen rearrangement and an epoxide opening/allylsilylation reaction installed the contiguous all-carbon quaternary stereocentres with the required relative stereochemistry.
September 14, 2016: Chemical Communications: Chem Comm
Stell Patadji, Joshua Pantanowitz, Natalie M Perlov, Sara E Monaco
INTRODUCTION: Arborizing stromal meshwork fragments (ASMFs) have been proposed as a useful clue to differentiate mucin associated with mucinous adenocarcinoma from contaminating mucus during endobronchial ultrasound-guided transbronchial needle aspiration. Our aim was to retrospectively review cytology cases with mucinous material to determine the utility of ASMFs in diagnosing mucinous tumors. MATERIAL AND METHODS: Diff-Quik stained smears from archival cytology cases (N = 40) were reviewed, including adenocarcinomas with mucinous features, cystic mucinous neoplasms, and control cases with mucin contamination...
October 2016: Diagnostic Cytopathology
Malte B Monin, Petra Krause, Robin Stelling, Derya Bocuk, Sabine Niebert, Florian Klemm, Tobias Pukrop, Sarah Koenig
BACKGROUND: Wnt/β-catenin signaling is known to play an important role in colorectal cancer (CRC). Niclosamide, a salicylamide derivative used in the treatment of tapeworm infections, targets the Wnt/β-catenin pathway. The objective of this study was to investigate niclosamide as a therapeutic agent against CRC. METHODS: The antiproliferative effects of 1, 3, 10, and 50 μM concentrations of niclosamide on human (SW480 and SW620) and rodent (CC531) CRC cell lines were determined by MTS assay and direct cell count...
June 1, 2016: Journal of Surgical Research
Robert Gnügge, Lekshmi Dharmarajan, Moritz Lang, Jörg Stelling
Feedback loops in biological networks, among others, enable differentiation and cell cycle progression, and increase robustness in signal transduction. In natural networks, feedback loops are often complex and intertwined, making it challenging to identify which loops are mainly responsible for an observed behavior. However, minimal synthetic replicas could allow for such identification. Here, we engineered a synthetic permease-inducer-repressor system in Saccharomyces cerevisiae to analyze if a transport-mediated positive feedback loop could be a core mechanism for the switch-like behavior in the regulation of metabolic gene networks such as the S...
October 21, 2016: ACS Synthetic Biology
Dinesh Pradhan, Zaibo Li, Rebecca Ocque, Stell Patadji, Chengquan Zhao
BACKGROUND: The interpretation of atypical glandular cells (AGC) in Papanicolaou (Pap) tests and screening for glandular neoplasia remain challenging. METHODS: Pap tests with an AGC interpretation from January 2008 to December 2013 were retrieved from a pathology database, and cases with histologic follow-up within 1 year were included in the study. RESULTS: There were 589,830 Pap tests during the study period, and 3709 cases (0.6%) were reported as AGC, which included the following: AGC, not otherwise specified (52...
August 2016: Cancer Cytopathology
Mariana A Soares, Felipe C O B Teixeira, Miguel Fontes, Ana Lúcia Arêas, Marcelo G Leal, Mauro S G Pavão, Mariana P Stelling
The metastatic disease is one of the main consequences of tumor progression, being responsible for most cancer-related deaths worldwide. This review intends to present and discuss data on the relationship between integrins and heparan sulfate proteoglycans in health and cancer progression. Integrins are a family of cell surface transmembrane receptors, responsible for cell-matrix and cell-cell adhesion. Integrins' main functions include cell adhesion, migration, and survival. Heparan sulfate proteoglycans (HSPGs) are cell surface molecules that play important roles as cell receptors, cofactors, and overall direct or indirect contributors to cell organization...
2015: BioMed Research International
Maria Mellinas-Gomez, Victoria J Spanswick, Solange R Paredes-Moscosso, Matthew Robson, R Barbara Pedley, David E Thurston, Stephen J Baines, Anneliese Stell, John A Hartley
BACKGROUND: Cancer is the leading cause of death in older dogs and its prevalence is increasing. There is clearly a need to develop more effective anti-cancer drugs in dogs. SG2000 (SJG-136) is a sequence selective DNA minor groove cross-linking agent. Based on its in vitro potency, the spectrum of in vivo and clinical activity against human tumours, and its tolerability in human patients, SG2000 has potential as a novel therapeutic against spontaneously occurring canine malignancies...
August 19, 2015: BMC Veterinary Research
Ajeet Kumar Gandhi, Soumyajit Roy, Ahitagni Biswas, Mohd Waseem Raza, Tripti Saxena, Suman Bhasker, Atul Sharma, Alok Thakar, Bidhu Kalyan Mohanti
OBJECTIVE: Carcinoma of external auditory canal (EAC) is a rare disease with variable management strategies and prognosis. We aimed to analyze treatment modalities, prognostic factors and survival outcomes in patients of squamous cell carcinoma of EAC treated at our institution. METHODS: Forty-three patients of squamous cell carcinoma of EAC were analyzed for clinical presentation, stage, surgical procedures and radiotherapy (RT) modalities employed. Stell and McCormick staging system was used for staging of the patients...
February 2016: Auris, Nasus, Larynx
Ana Paula Oliveira, Sotiris Dimopoulos, Alberto Giovanni Busetto, Stefan Christen, Reinhard Dechant, Laura Falter, Morteza Haghir Chehreghani, Szymon Jozefczuk, Christina Ludwig, Florian Rudroff, Juliane Caroline Schulz, Asier González, Alexandre Soulard, Daniele Stracka, Ruedi Aebersold, Joachim M Buhmann, Michael N Hall, Matthias Peter, Uwe Sauer, Jörg Stelling
Cells react to nutritional cues in changing environments via the integrated action of signaling, transcriptional, and metabolic networks. Mechanistic insight into signaling processes is often complicated because ubiquitous feedback loops obscure causal relationships. Consequently, the endogenous inputs of many nutrient signaling pathways remain unknown. Recent advances for system-wide experimental data generation have facilitated the quantification of signaling systems, but the integration of multi-level dynamic data remains challenging...
April 2015: Molecular Systems Biology
D R Killick, A J Stell, B Catchpole
Over the last 50 years, the significance of the immune system in the development and control of cancer has been much debated. However, recent discoveries provide evidence for a role of immunological mechanisms in the detection and destruction of cancer cells. Forty years ago veterinary oncologists were already investigating the feasibility of treating neoplasia by enhancing anticancer immunity. Unfortunately, this research was hindered by lack of a detailed understanding of cancer immunology, this limited the specificity and success of these early approaches...
April 2015: Journal of Small Animal Practice
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