keyword
MENU ▼
Read by QxMD icon Read
search

Flotillin

keyword
https://www.readbyqxmd.com/read/29679947/tissue-factor-coagulant-activity-is-regulated-by-the-plasma-membrane-microenvironment
#1
Yuanjie Yu, Anita N Böing, Chi M Hau, Najat Hajji, Wolfram Ruf, Auguste Sturk, Rienk Nieuwland
BACKGROUND:  Tissue factor (TF) can be present in a non-coagulant and coagulant form. Whether the coagulant activity is affected by the plasma membrane microenvironment is unexplored. OBJECTIVE:  This article studies the presence and coagulant activity of human TF in plasma membrane micro-domains. METHODS:  Plasma membranes were isolated from human MIA PaCa2 cells, MDA-MB-231 cells and human vascular smooth muscle cells by Percoll gradient ultracentrifugation after cell disruption...
April 21, 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29669282/an-unusual-prohibitin-regulates-malaria-parasite-mitochondrial-membrane-potential
#2
Joachim Michael Matz, Christian Goosmann, Kai Matuschewski, Taco Wilhelmus Antonius Kooij
Proteins of the stomatin/prohibitin/flotillin/HfIK/C (SPFH) family are membrane-anchored and perform diverse cellular functions in different organelles. Here, we investigate the SPFH proteins of the murine malaria model parasite Plasmodium berghei, the conserved prohibitin 1, prohibitin 2, and stomatin-like protein and an unusual prohibitin-like protein (PHBL). The SPFH proteins localize to the parasite mitochondrion. While the conserved family members could not be deleted from the Plasmodium genome, PHBL was successfully ablated, resulting in impaired parasite fitness and attenuated virulence in the mammalian host...
April 17, 2018: Cell Reports
https://www.readbyqxmd.com/read/29667320/flotillin-1-interacts-with-the-serotonin-transporter-and-modulates-chronic-corticosterone-response
#3
Sonali N Reisinger, Eryan Kong, Barbara Molz, Thomas Humberg, Spyros Sideromenos, Ana Cicvaric, Thomas Steinkellner, Jae-Won Yang, Maureen Cabatic, Francisco J Monje, Harald H Sitte, Benjamin J Nichols, Daniela D Pollak
Aberrant serotonergic neurotransmission in the brain is considered at the core of the pathophysiological mechanisms involved in neuropsychiatric disorders. Gene by environment interactions contribute to the development of depression and involve modulation of the availability and functional activity of the serotonin transporter (SERT). Using behavioral and in vivo electrophysiological approaches together with biochemical, molecular-biological and molecular imaging tools we establish Flotillin-1 (Flot1) as a novel protein interacting with SERT and demonstrate its involvement in the response to chronic corticosterone (CORT) treatment...
April 18, 2018: Genes, Brain, and Behavior
https://www.readbyqxmd.com/read/29642469/flotillins-regulate-focal-adhesions-by-interacting-with-%C3%AE-actinin-and-by-influencing-the-activation-of-focal-adhesion-kinase
#4
Antje Banning, Tanja Babuke, Nina Kurrle, Melanie Meister, Mika O Ruonala, Ritva Tikkanen
Cell-matrix adhesion and cell migration are physiologically important processes that also play a major role in cancer spreading. In cultured cells, matrix adhesion depends on integrin-containing contacts such as focal adhesions. Flotillin-1 and flotillin-2 are frequently overexpressed in cancers and are associated with poor survival. Our previous studies have revealed a role for flotillin-2 in cell-matrix adhesion and in the regulation of the actin cytoskeleton. We here show that flotillins are important for cell migration in a wound healing assay and influence the morphology and dynamics of focal adhesions...
April 7, 2018: Cells
https://www.readbyqxmd.com/read/29623357/membrane-microdomains-regulate-nlrp10-and-nlrp12-dependent-signalling-in-a549-cells-challenged-with-cigarette-smoke-extract
#5
Dhirendra P Singh, Gagandeep Kaur, Prathyusha Bagam, Rakeysha Pinkston, Sanjay Batra
Chronic obstructive pulmonary disease (COPD) is predicted to become the third leading cause of death and disability worldwide by 2030; with cigarette smoking (active or passive) being one of the chief cause of its occurrence. Cigarette smoke exposure has been found to result in excessive inflammation and tissue injury, which might lead to COPD, although the exact pathophysiology of the disease remains elusive. While previous studies have demonstrated the role of membrane-bound Toll-like receptors (TLRs) in cigarette smoke (CS)-induced inflammation, scant information is available about the role of cytosolic NOD-like receptors (NLRs) in regulating CS-mediated inflammatory responses...
April 6, 2018: Archives of Toxicology
https://www.readbyqxmd.com/read/29521294/effects-of-adipose-derived-mesenchymal-stem-cell-exosomes-on-corneal-stromal-fibroblast-viability-and-extracellular-matrix-synthesis
#6
Ting Shen, Qing-Qing Zheng, Jiang Shen, Qiu-Shi Li, Xing-Hui Song, Hong-Bo Luo, Chao-Yang Hong, Ke Yao
Background: Corneal stromal cells (CSCs) are components of the corneal endothelial microenvironment that can be induced to form a functional tissue-engineered corneal endothelium. Adipose-derived mesenchymal stem cells (ADSCs) have been reported as an important component of regenerative medicine and cell therapy for corneal stromal damage. We have demonstrated that the treatment with ADSCs leads to phenotypic changes in CSCs in vitro. However, the underlying mechanisms of such ADSC-induced changes in CSCs remain unclear...
March 20, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29504311/roles-of-flotillins-in-tumors
#7
Xu-Xu Liu, Wei-Dong Liu, Lei Wang, Bin Zhu, Xiao Shi, Zi-Xuan Peng, He-Cheng Zhu, Xing-Dong Liu, Mei-Zuo Zhong, Dan Xie, Mu-Sheng Zeng, Cai-Ping Ren
The identification and use of molecular biomarkers have greatly improved the diagnosis and treatment of malignant tumors. However, a much deeper understanding of oncogenic proteins is needed for the benefit to cancer patients. The lipid raft marker proteins, flotillin-1 and flotillin-2, were first found in goldfish retinal ganglion cells during axon regeneration. They have since been found in a variety of cells, mainly on the inner surface of cell membranes, and not only act as a skeleton to provide a platform for protein-protein interactions, but also are involved in signal transduction, nerve regeneration, endocytosis, and lymphocyte activation...
2018: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/29499201/prognostic-value-of-flotillins-flotillin-1-and-flotillin-2-in-human-cancers-a-meta-analysis
#8
Yujiao Deng, Pengbo Ge, Tian Tian, Cong Dai, Meng Wang, Shuai Lin, Kang Liu, Yi Zheng, Peng Xu, Linghui Zhou, Qian Hao, Zhijun Dai
Increasing evidence indicates that flotillins which associate with cell infiltration and metastasis are overexpressed in multiple tumors. The prognostic role of flotillins remains controversial. We conducted a comprehensive meta-analysis of published research to investigate the prognostic value of flotillins in patients with cancer. Pooled HRs (hazard ratio) with 95% CIs (confidence interval) were collected to estimate the prognostic value. Twenty-seven studies with 4803 cancer patients were finally identified...
February 27, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29389961/the-candida-albicans-stress-response-gene-stomatin-like-protein-3-is-implicated-in-ros-induced-apoptotic-like-death-of-yeast-phase-cells
#9
Karen A Conrad, Ronald Rodriguez, Eugenia C Salcedo, Jason M Rauceo
The ubiquitous presence of SPFH (Stomatin, Prohibitin, Flotillin, HflK/HflC) proteins in all domains of life suggests that their function would be conserved. However, SPFH functions are diverse with organism-specific attributes. SPFH proteins play critical roles in physiological processes such as mechanosensation and respiration. Here, we characterize the stomatin ORF19.7296/SLP3 in the opportunistic human pathogen Candida albicans. Consistent with the localization of stomatin proteins, a Slp3p-Yfp fusion protein formed visible puncta along the plasma membrane...
2018: PloS One
https://www.readbyqxmd.com/read/29353037/curcumin-protects-cortical-neurons-against-oxygen-and-glucose-deprivation-reoxygenation-injury-through-flotillin-1-and-extracellular-signal-regulated-kinase1-2-pathway
#10
Zhengyu Lu, Yanping Liu, Yang Shi, Xinjie Shi, Xin Wang, Chuan Xu, Hong Zhao, Qiang Dong
In this study, we provided evidence that curcumin could be a promising therapeutic agent for ischemic stroke by activating neuroprotective signaling pathways. Post oxygen and glucose deprivation/reoxygenation (OGD/R), primary mouse cortical neurons treated with curcumin exhibited a significant decrease in cell death, LDH release and enzyme caspase-3 activity under OGD/R circumstances, which were abolished by flotillin-1 downregulation or extracellular signal-regulated kinase (ERK) inhibitor. Moreover, flotillin-1 knockdown led to suppression of curcumin-mediated ERK phosphorylation under OGD/R condition...
February 5, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29343516/stat3-mediated-alterations-in-lysosomal-membrane-protein-composition
#11
Bethan Lloyd-Lewis, Caroline C Krueger, Timothy J Sargeant, Michael E D'Angelo, Michael J Deery, Renata Feret, Julie A Howard, Kathryn S Lilley, Christine J Watson
Lysosome function is essential in cellular homeostasis. In addition to its recycling role, the lysosome has recently been recognised as a cellular signalling hub. We have shown in mammary epithelial cells, both in vivo and in vitro, that signal transducer and activator of transcription 3 (Stat3) modulates lysosome biogenesis and can promote the release of lysosomal proteases that culminates in cell death. To further investigate the impact of Stat3 on lysosomal function, we have conducted a proteomic screen of changes in lysosomal membrane protein components induced by Stat3 using an iron nanoparticle enrichment strategy...
January 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29170640/shedding-of-microvesicles-from-microglia-contributes-to-the-effects-induced-by-metabotropic-glutamate-receptor-5-activation-on-neuronal-death
#12
Martina Beneventano, Simona F Spampinato, Sara Merlo, Mariangela Chisari, Paola Platania, Marco Ragusa, Michele Purrello, Ferdinando Nicoletti, Maria Angela Sortino
Metabotropic glutamate (mGlu) receptor 5 is involved in neuroinflammation and has been shown to mediate reduced inflammation and neurotoxicity and to modify microglia polarization. On the other hand, blockade of mGlu5 receptor results in inhibition of microglia activation. To dissect this controversy, we investigated whether microvesicles (MVs) released from microglia BV2 cells could contribute to the communication between microglia and neurons and whether this interaction was modulated by mGlu5 receptor. Activation of purinergic ionotropic P2X7 receptor with the stable ATP analog benzoyl-ATP (100 μM) caused rapid MVs shedding from BV2 cells...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29166667/flotillin-scaffold-activity-contributes-to-type-vii-secretion-system-assembly-in-staphylococcus-aureus
#13
Benjamin Mielich-Süss, Rabea M Wagner, Nicole Mietrach, Tobias Hertlein, Gabriella Marincola, Knut Ohlsen, Sebastian Geibel, Daniel Lopez
Scaffold proteins are ubiquitous chaperones that promote efficient interactions between partners of multi-enzymatic protein complexes; although they are well studied in eukaryotes, their role in prokaryotic systems is poorly understood. Bacterial membranes have functional membrane microdomains (FMM), a structure homologous to eukaryotic lipid rafts. Similar to their eukaryotic counterparts, bacterial FMM harbor a scaffold protein termed flotillin that is thought to promote interactions between proteins spatially confined to the FMM...
November 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29162604/suppression-of-hepatic-flot1-flotillin-1-by-type-2-diabetes-mellitus-impairs-the-disposal-of-remnant-lipoproteins-via-syndecan-1
#14
Keyang Chen, Qingsi Wu, Kongwang Hu, Chengwei Yang, Xiangdong Wu, Peter Cheung, Kevin Jon Williams
OBJECTIVE: Type 2 diabetes mellitus (T2DM) and the atherometabolic syndrome exhibit a deadly dyslipoproteinemia that arises in part from impaired hepatic disposal of C-TRLs (cholesterol- and triglyceride-rich remnant apoB [apolipoprotein B] lipoproteins). We previously identified syndecan-1 as a receptor for C-TRLs that directly mediates endocytosis via rafts, independent from coated pits. Caveolins and flotillins form rafts but facilitate distinct endocytotic pathways. We now investigated their participation in syndecan-1-mediated disposal of C-TRLs and their expression in T2DM liver...
January 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29103614/membrane-microdomain-disassembly-inhibits-mrsa-antibiotic-resistance
#15
Esther García-Fernández, Gudrun Koch, Rabea M Wagner, Agnes Fekete, Stephanie T Stengel, Johannes Schneider, Benjamin Mielich-Süss, Sebastian Geibel, Sebastian M Markert, Christian Stigloher, Daniel Lopez
A number of bacterial cell processes are confined functional membrane microdomains (FMMs), structurally and functionally similar to lipid rafts of eukaryotic cells. How bacteria organize these intricate platforms and what their biological significance is remain important questions. Using the pathogen methicillin-resistant Staphylococcus aureus (MRSA), we show here that membrane-carotenoid interaction with the scaffold protein flotillin leads to FMM formation, which can be visualized using super-resolution array tomography...
November 30, 2017: Cell
https://www.readbyqxmd.com/read/29063370/pathways-of-production-and-delivery-of-hepatocyte-exosomes
#16
Li Chen, Ruju Chen, Sherri Kemper, David R Brigstock
Hepatocyte exosomes (ExoHep ) are proposed to mediate physiological or pathophysiological signaling in a variety of hepatic target cells. ExoHep were purified from the medium of primary mouse hepatocytes or AML12 cells and characterized as ~100 nm nanovesicles that were positive for proteins commonly found in exosomes (CD9, CD81, flotillin) or hepatocytes (asialoglycoprotein receptor). Ethanol treatment of hepatocytes caused increased ExoHep release and increased cellular mRNA expression of components involved in intracellular vesicle trafficking (Rab 5a,b,c, Rab 7a, Rab 27a,b) or exosome biogenesis via the ESCRT (HGS, Alix, STAM1, TSG101, VTA1, YKT6) or ceramide (nSmase2) pathways...
March 2018: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/29051303/transport-associated-pathway-responses-in-ovine-fetal-membranes-to-changes-in-amniotic-fluid-dynamics
#17
Cecilia Y Cheung, Debra F Anderson, Robert A Brace
Current evidence suggests that amniotic fluid volume (AFV) is actively regulated by vesicular transport of amniotic fluid outward across the amnion and into the underlying fetal vasculature in the placenta. Our objective was to determine whether gene expression profiles of potential stimulators, inhibitors, and mediators of vesicular transport are altered in response to changes in intramembranous absorption (IMA) rate. Samples of ovine amnion and chorion were obtained from fetal sheep with normal, experimentally reduced or increased AFVs and IMA rates...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29030127/a-method-for-the-isolation-and-enrichment-of-purified-bovine-milk-exosomes
#18
Kanchan Vaswani, Yong Qin Koh, Fatema B Almughlliq, Hassendrini N Peiris, Murray D Mitchell
Exosomes are nanovesicles that play important roles in intercellular communication as they carry information to target cells. Isolation of high purity exosomes will aid in studying the exosomal cargo and quantity as well as how cell-specific messages are carried. We describe a new method incorporating size exclusion chromatography (SEC) to enrich milk-derived exosomes from extracellular vesicles (EVs). This involved the initial isolation of EVs from bovine milk via milk processing and ultracentrifugation; followed by a new method to enrich exosomes using SEC...
December 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29020490/impact-of-ticagrelor-on-p2y1-and-p2y12-localization-and-on-cholesterol-levels-in-platelet-plasma-membrane
#19
Vahideh Rabani, Damien Montange, Nicolas Meneveau, Siamak Davani
Ticagrelor is an antiplatelet agent that inhibits platelet activation via P2Y12 antagonism. There are several studies showing that P2Y12 needs lipid rafts to be activated, but there are few data about how ticagrelor impacts lipid raft organization. Therefore, we aimed to investigate how ticagrelor could impact the distribution of cholesterol and consequently alter the organization of lipid rafts on platelet plasma membranes. We identified cholesterol-enriched raft fractions in platelet membranes by quantification of their cholesterol levels...
October 11, 2017: Platelets
https://www.readbyqxmd.com/read/28930577/exosome-enrichment-by-ultracentrifugation-and-size-exclusion-chromatography
#20
Yong Qin Koh, Fatema B Almughlliq, Kanchan Vaswani, Hassendrini N Peiris, Murray D Mitchell
Exosomes are a subset of extracellular vesicles (EVs) that have important roles in intercellular communication. They contain and carry bioactive molecules within their membranes which are delivered to target cells. Reproducible isolation and enrichment of these exosomes will aid in evaluation of cellular communication. We present an approach that involved the pre-processing of plasma, combined with ultracentrifugation (UC) and size exclusion chromatography (SEC) to isolate EVs and subsequently enrich exosomes...
January 1, 2018: Frontiers in Bioscience (Landmark Edition)
keyword
keyword
17509
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"