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https://www.readbyqxmd.com/read/28225561/flotillins-control-zebrafish-epiboly-through-their-role-in-cadherin-mediated-cell-cell-adhesion
#1
Eduardo A Rios Morris, Stéphane Bodin, Bénédicte Delaval, Franck Comunale, Virginie Georget, Manoel L Costa, Georges Lutfalla, Cécile Gauthier-Rouvière
Zebrafish gastrulation and particularly epiboly that involves coordinated movements of several cell layers is a dynamic process for which regulators remain to be identified. We show here that Flotillin 1 and 2, ubiquitous and highly conserved proteins, are required for epiboly. Flotillins knockdown compromised embryo survival, strongly delayed epiboly and impaired deep cell radial intercalation and directed collective migration without affecting enveloping layer cell movement. At the molecular level, we identified that Flotillins are required for the formation of E-cadherin-mediated cell-cell junctions...
February 22, 2017: Biology of the Cell
https://www.readbyqxmd.com/read/28188157/microrna-802-inhibits-epithelial-mesenchymal-transition-through-targeting-flotillin-2-in-human-prostate-cancer
#2
Dawei Wang, Guoliang Lu, Yuan Shao, Da Xu
Objective: MicroRNAs (miRNAs) are a class of non-coding RNAs that exert critical roles in various biological processes. The target of the present study was to identify the functional roles of microRNA-802 (miR-802) in regulating epithelial-mesenchymal transition (EMT) in prostate cancer (PCa). Methods: miR-802 expression was investigated in 73 pairs of PCa samples and PCa cell lines (PC3 and DU145 cells) by qRT-PCR. Cell proliferation was detected using MTT assay, and cell apoptosis was evaluated using flow cytometry...
February 10, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28148778/lipid-rafts-of-mouse-liver-contain-nonextended-and-extended-acetylcholinesterase-variants-along-with-m3-muscarinic-receptors
#3
María Fernanda Montenegro, Juan Cabezas-Herrera, F Javier Campoy, Encarnación Muñoz-Delgado, Cecilio J Vidal
The observation of acetylcholinesterase (AChE) type H (AChEH), which is the predominant AChE variant in visceral organs and immune cells, in lipid rafts of muscle supports functional reasons for the raft targeting of glypiated AChEH The search for these reasons revealed that liver AChE activity is mostly confined to rafts and that the liver is able to make N-extended AChE variants and target them to rafts. These results prompted us to test whether AChE and muscarinic receptors existed in the same raft. Isolation of flotillin-2-rich raft fractions by their buoyancy in sucrose gradients, followed by immunoadsorption and matrix-assisted laser desorption ionization-time of flight-mass spectrometry application, gave the following results: 1) most hepatic AChE activity emanates from AChE-H mRNA, and its product, glypiated AChEH, accumulates in rafts; 2) N-extended N-AChE readthrough variant, nonglypiated N-AChEH, and N-AChE tailed variant were all identified in liver rafts; and 3) M3 AChRs were observed in rafts, and coprecipitation of raft-confined N-AChE and M3 receptors by using anti-M3 antibodies showed that enzyme and receptor reside in the same raft unit...
February 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28146372/the-isolation-of-morphologically-intact-and-biologically-active-extracellular-vesicles-from-the-secretome-of-cancer-associated-adipose-tissue
#4
Sarah Jeurissen, Glenn Vergauwen, Jan Van Deun, Lore Lapeire, Victoria Depoorter, Ilkka Miinalainen, Raija Sormunen, Rudy Van den Broecke, Geert Braems, Véronique Cocquyt, Hannelore Denys, An Hendrix
Breast cancer cells closely interact with different cell types of the surrounding adipose tissue to favor invasive growth and metastasis. Extracellular vesicles (EVs) are nanometer-sized vesicles secreted by different cell types that shuttle proteins and nucleic acids to establish cell-cell communication. To study the role of EVs released by cancer-associated adipose tissue in breast cancer progression and metastasis a standardized EV isolation protocol that obtains pure EVs and maintains their functional characteristics is required...
February 1, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/28042053/potential-role-for-the-vdr-agonist-elocalcitol-in-metabolic-control-evidences-in-human-skeletal-muscle-cells
#5
Cristina Antinozzi, Clarissa Corinaldesi, Carla Giordano, Annalinda Pisano, Bruna Cerbelli, Silvia Migliaccio, Luigi Di Luigi, Katia Stefanantoni, Gabriella Barbara Vannelli, Salvatore Minisola, Guido Valesini, Valeria Riccieri, Andrea Lenzi, Clara Crescioli
Vitamin D plays a pivotal role to maintain skeletal muscle integrity and health. Vitamin D deficiency characterizes inflammatory myopathy (IM) and diabetes, often overlapping diseases involving skeletal muscle damage. Vitamin D receptor (VDR) agonists likely exert beneficial effects in both IM and metabolic disturbances. We aim to evaluate in vitro the effect of elocalcitol, a non-hypercalcemic VDR agonist, on the biomolecular metabolic machinery of human skeletal muscle cells (Hfsmc), vs. insulin (I). We analyzed GLUT4, Flotillin-1, Caveolin-3 and Caveolin-1 cell expression/localization; mTOR, AKT, ERK and 4E-BP1 phosphorylation; IL-6 myokine release; VDR expression...
March 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28036279/proteins-that-interact-with-calgranulin-b-in-the-human-colon-cancer-cell-line-hct-116
#6
Jae Kyung Myung, Seung-Gu Yeo, Kyung Hee Kim, Kwang-Soo Baek, Daye Shin, Jong Heon Kim, Jae Youl Cho, Byong Chul Yoo
Calgranulin B is released from immune cells and can be internalized into colon cancer cells to prevent proliferation. The present study aimed to identify proteins that interact with calgranulin B to suppress the proliferation of colon cancer cells, and to obtain information on the underlying anti-tumor mechanism(s) of calgranulin B. Calgranulin B expression was induced in colon cancer cell line HCT-116 by infection with calgranulin B-FLAG expressing lentivirus, and it led to a significant suppression of cell proliferation...
January 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/27993509/reggie-1-and-reggie-2-flotillins-participate-in-rab11a-dependent-cargo-trafficking-spine-synapse-formation-and-ltp-related-ampa-receptor-glua1-surface-exposure-in-mouse-hippocampal-neurons
#7
Vsevolod Bodrikov, Aline Pauschert, Gaga Kochlamazashvili, Claudia A O Stuermer
Reggie-1 and -2 (flotillins) reside at recycling vesicles and promote jointly with Rab11a the targeted delivery of cargo. Recycling is essential for synapse formation suggesting that reggies and Rab11a may regulate the development of spine synapses. Recycling vesicles provide cargo for dendritic growth and recycle surface glutamate receptors (AMPAR, GluA) for long-term potentiation (LTP) induced surface exposure. Here, we show reduced number of spine synapses and impairment of an in vitro correlate of LTP in hippocampal neurons from reggie-1 k...
December 16, 2016: Experimental Neurology
https://www.readbyqxmd.com/read/27943211/purification-and-analysis-of-exosomes-released-by-mature-cortical-neurons-following-synaptic-activation
#8
Karine Laulagnier, Charlotte Javalet, Fiona J Hemming, Rémy Sadoul
Exosomes are vesicles released by most cells into their environment upon fusion of multivesicular endosomes with the plasma membrane. Exosomes are vesicles of 60-100 nm in diameter, floating in sucrose at a density of ~1.15 g/mL and carrying a number of marker proteins such as Alix, Tsg101, and Flotillin-1. We use dissociated cortical neurons cultured for around two weeks as exosome-releasing cells. In these conditions, neurons make mature synapses and form networks that can be activated by physiological stimuli...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27881660/the-norovirus-ns3-protein-is-a-dynamic-lipid-and-microtubule-associated-protein-involved-in-viral-rna-replication
#9
Ben T Cotton, Jennifer L Hyde, Soroush T Sarvestani, Stanislav V Sosnovtsev, Kim Y Green, Peter A White, Jason M Mackenzie
: Norovirus (NoV) infections are a significant health burden to society, yet the lack of reliable tissue culture systems has hampered the development of appropriate antiviral therapies. Here we show that the NoV NS3 protein, derived from murine NoV (MNV), is intimately associated with the MNV replication complex and the viral replication intermediate double-stranded RNA (dsRNA). We observed that when expressed individually, MNV NS3 and NS3 encoded by human Norwalk virus (NV) induced the formation of distinct vesicle-like structures that did not colocalize with any particular protein markers to cellular organelles but localized to cellular membranes, in particular those with a high cholesterol content...
February 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27738004/gef-h1-is-necessary-for-neutrophil-shear-stress-induced-migration-during-inflammation
#10
Noah Fine, Ioannis D Dimitriou, Jacob Rullo, María José Sandí, Björn Petri, Jack Haitsma, Hisham Ibrahim, Jose La Rose, Michael Glogauer, Paul Kubes, Myron Cybulsky, Robert Rottapel
Leukocyte crawling and transendothelial migration (TEM) are potentiated by shear stress caused by blood flow. The mechanism that couples shear stress to migration has not been fully elucidated. We found that mice lacking GEF-H1 (GEF-H1(-/-)), a RhoA-specific guanine nucleotide exchange factor (GEF), displayed limited migration and recruitment of neutrophils into inflamed tissues. GEF-H1(-/-) leukocytes were deficient in in vivo crawling and TEM in the postcapillary venules. We demonstrated that although GEF-H1 deficiency had little impact on the migratory properties of neutrophils under static conditions, shear stress triggered GEF-H1-dependent spreading and crawling of neutrophils and relocalization of GEF-H1 to flotillin-2-rich uropods...
October 10, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27737933/the-membrane-scaffold-slp2-anchors-a-proteolytic-hub-in-mitochondria-containing-parl-and-the-i-aaa-protease-yme1l
#11
Timothy Wai, Shotaro Saita, Hendrik Nolte, Sebastian Müller, Tim König, Ricarda Richter-Dennerlein, Hans-Georg Sprenger, Joaquin Madrenas, Mareike Mühlmeister, Ulrich Brandt, Marcus Krüger, Thomas Langer
The SPFH (stomatin, prohibitin, flotillin, HflC/K) superfamily is composed of scaffold proteins that form ring-like structures and locally specify the protein-lipid composition in a variety of cellular membranes. Stomatin-like protein 2 (SLP2) is a member of this superfamily that localizes to the mitochondrial inner membrane (IM) where it acts as a membrane organizer. Here, we report that SLP2 anchors a large protease complex composed of the rhomboid protease PARL and the i-AAA protease YME1L, which we term the SPY complex (for SLP2-PARL-YME1L)...
December 2016: EMBO Reports
https://www.readbyqxmd.com/read/27689006/depot-specific-differences-in-the-adipogenic-potential-of-precursors-are-mediated-by-collagenous-extracellular-matrix-and-flotillin-2%C3%A2-dependent-signaling
#12
Gerald Grandl, Sebastian Müller, Hansjörg Moest, Caroline Moser, Bernd Wollscheid, Christian Wolfrum
OBJECTIVE: Adipose tissue shows a high degree of plasticity, and adipocyte hyperplasia is an important mechanism for adipose tissue expansion. Different adipose depots respond differently to an increased demand for lipid storage. Orchestrating cellular expansion in vivo requires extracellular matrix (ECM) remodeling and a high degree of interaction between cells and ECM. METHODS: We studied decellularized primary adipose stromal cell derived ECM of different adipose depots and reseeded them with primary adipose precursors...
October 2016: Molecular Metabolism
https://www.readbyqxmd.com/read/27676653/a-novel-role-for-flotillin-containing-lipid-rafts-in-negative-feedback-regulation-of-thyroid-specific-gene-expression-by-thyroglobulin
#13
Yuqian Luo, Takeshi Akama, Akiko Okayama, Aya Yoshihara, Mariko Sue, Kenzaburo Oda, Moyuru Hayashi, Yuko Ishido, Hisashi Hirano, Naoki Hiroi, Ryohei Katoh, Koichi Suzuki
BACKGROUND: Thyroglobulin (Tg) stored in thyroid follicles regulates follicular function in thyroid hormone (TH) synthesis by suppressing thyroid-specific gene expression in a concentration-dependent manner. Thus, Tg is an intrinsic negative-feedback regulator that can restrain the effect of thyroid stimulating hormone (TSH) in the follicle. However, the underlying mechanisms by which Tg exerts its prominent autoregulatory effect following recognition by thyrocytes remains unclear. METHODS: To identify potential proteins that recognize and interact with Tg, mass spectrometry (MS) was used to analyze immunoprecipitated Tg-bound proteins derived from Tg-treated rat thyroid FRTL-5 cells...
September 27, 2016: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/27664330/exosomal-proteins-as-prostate-cancer-biomarkers-in-urine-from-mass-spectrometry-discovery-to-immunoassay-based-validation
#14
Ling Wang, Tore Skotland, Viktor Berge, Kirsten Sandvig, Alicia Llorente
Exosomes have recently appeared as a novel source of non-invasive cancer biomarkers since tumor-specific molecules can be found in exosomes isolated from biological fluids. We have previously analyzed the proteome of urinary exosomes by mass spectrometry, and identified proteins differentially expressed in prostate cancer patients compared to healthy males. Since mass spectrometry is so far not commonly used in clinical laboratories, we have here investigated whether antibody-based methods such as Western blot or ELISA can be used to validate the use of the identified proteins as prostate cancer biomarkers...
September 21, 2016: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27601599/comprehensive-proteomic-analysis-of-human-milk-derived-extracellular-vesicles-unveils-a-novel-functional-proteome-distinct-from-other-milk-components
#15
Martijn J C van Herwijnen, Marijke I Zonneveld, Soenita Goerdayal, Esther N M Nolte-'t Hoen, Johan Garssen, Bernd Stahl, A F Maarten Altelaar, Frank A Redegeld, Marca H M Wauben
Breast milk contains several macromolecular components with distinctive functions, whereby milk fat globules and casein micelles mainly provide nutrition to the newborn, and whey contains molecules that can stimulate the newborn's developing immune system and gastrointestinal tract. Although extracellular vesicles (EV) have been identified in breast milk, their physiological function and composition has not been addressed in detail. EV are submicron sized vehicles released by cells for intercellular communication via selectively incorporated lipids, nucleic acids, and proteins...
November 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27588480/lipid-rafts-promote-liver-cancer-cell-proliferation-and-migration-by-up-regulation-of-tlr7-expression
#16
Yuan Liu, Xiaodong Guo, Liyuan Wu, Mei Yang, Zhiwei Li, Yinjie Gao, Shuhong Liu, Guangde Zhou, Jingmin Zhao
: Hepatocellular carcinoma (HCC) occurs predominantly in patients with underlying chronic liver disease and cirrhosis. Toll-like receptors (TLRs) play an important role in innate immune responses and TLR signaling has been associated with various chronic liver diseases. Lipid rafts provide the necessary microenvironment for certain specialized signaling events to take place, such as the innate immune recognition. The purpose of this study was to determine the pattern of TLR7 expression in HCC, how to recruit TLR7 into lipid rafts responded to ligands and whether targeting TLR7 might have beneficial effects...
September 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/27585903/endocytic-transport-of-polyplex-and-lipoplex-sirna-vectors-in-hela-cells
#17
Mihael Lazebnik, Rahul K Keswani, Daniel W Pack
PURPOSE: siRNA may be delivered as electrostatic complexes with cationic lipids (lipoplexes) or polycations (polyplexes). The purpose of this project was to determine the effect of cellular internalization mechanism(s) on siRNA-mediated gene silencing efficiency. METHODS: Lipoplexes were formed comprising siRNA and N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (DOTAP), cholesterol and dioleoyl phosphatidylethanolamine (DOPE), and polyplexes comprised siRNA with polyethylenimine (PEI)...
September 1, 2016: Pharmaceutical Research
https://www.readbyqxmd.com/read/27558838/shiga-toxin-glycosphingolipid-receptors-and-their-lipid-membrane-ensemble-in-primary-human-blood-brain-barrier-endothelial-cells
#18
Nadine Legros, Stefanie Dusny, Hans-Ulrich Humpf, Gottfried Pohlentz, Helge Karch, Johannes Müthing
Shiga toxin (Stx)-mediated injury to microvascular endothelial cells in the brain significantly contributes to the pathogenesis of the hemolytic-uremic syndrome caused by enterohemorrhagic Escherichia coli (EHEC). Stxs are AB5 toxins and the B-pentamers of the two major Stx subtypes Stx1a and Stx2a preferentially bind to the glycosphingolipid (GSL) globotriaosylceramide (Gb3Cer) expressed by human endothelial cells. Here we report on comprehensive structural analysis of the different lipoforms of Gb3Cer (Galα4Galβ4Glcβ1Cer) and globotetraosylceramide (Gb4Cer, GalNAcβ3Galα4Galβ4Glcβ1Cer, the less effective Stx receptor) of primary human brain microvascular endothelial cells and their association with lipid rafts...
January 2017: Glycobiology
https://www.readbyqxmd.com/read/27448302/4-1n-is-involved-in-a-flotillin-1-%C3%AE-catenin-wnt-pathway-and-suppresses-cell-proliferation-and-migration-in-non-small-cell-lung-cancer-cell-lines
#19
Qin Yang, Min Zhu, Zi Wang, Hui Li, Weihua Zhou, Xiaojuan Xiao, Bin Zhang, Weixin Hu, Jing Liu
The membrane-cytoskeletal protein 4.1N has recently been proposed as a tumor suppressor in a number of cancers of epithelial origin, including non-small-cell lung cancer (NSCLC). However, the molecular mechanism associated with 4.1N tumor suppression remains has not been thoroughly characterized. In this study, 4.1N was shown to directly interact with the lipid raft marker flotillin-1 through its FERM and U2 domains in several different NSCLC cell lines using immunoprecipitation, co-immunoprecipitation and pull-down assays...
September 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27392863/amyloid-%C3%AE-reduces-exosome-release%C3%A2-from%C3%A2-astrocytes-by-enhancing-jnk-phosphorylation
#20
Mohammad Abdullah, Hiroshi Takase, Mari Nunome, Hiroyuki Enomoto, Jin-Ichi Ito, Jian-Sheng Gong, Makoto Michikawa
Exosomes are small extracellular vesicles secreted by variety of cell types such as neurons, astrocytes, and oligodendrocytes. It is suggested that exosomes play essential role in the maintenance of the neuronal functions and also in the clearance of amyloid-β (Aβ) from the brain. Aβ is well known to cause neuronal cell death, whereas little is known about its effect on astrocytes. In this study, we examined the effect of Aβ on release of exosomes from astrocytes in culture. We analyzed release of exosomes and apoE, both of which are known to remove/clear Aβ from the brain, in the culture medium of astrocytes...
July 2, 2016: Journal of Alzheimer's Disease: JAD
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