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https://www.readbyqxmd.com/read/29105943/characterization-of-printed-pla-scaffolds-for-bone-tissue-engineering
#1
Agathe Grémare, Vera Guduric, Reine Bareille, Valérie Heroguez, Simon Latour, Nicolas L'Heureux, Jean-Christophe Fricain, Sylvain Catros, Damien Le Nihouannen
Autografts remain the gold standard for orthopedic transplantations. However, to overcome its limitations, bone tissue engineering proposes new strategies. This includes the development of new biomaterials such as synthetic polymers, to serve as scaffold for tissue production. The objective of this present study was to produce poly(lactic) acid (PLA) scaffolds of different pore size using fused deposition modeling (FDM) technique and to evaluate their physicochemical and biological properties. Structural, chemical, mechanical and biological characterizations were performed...
November 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29054608/a-sweet-spot-for-fluid-induced-oscillations-in-the-conditioning-of-stem-cell-based-engineered-heart-valve-tissues
#2
Alexander Williams, Sana Nasim, Manuel Salinas, Arash Moshkforoush, Nikolaos Tsoukias, Sharan Ramaswamy
Fluid-induced shear stresses are involved in the development of cardiovascular tissues. In a tissue engineering framework, this stimulus has also been considered as a mechanical regulator of stem cell differentiation. We recently demonstrated that the fluid-oscillating effect in combination with a physiologically-relevant shear stress magnitude contributes to the formation of stem cell-derived de novo heart valve tissues. However, the range of oscillations necessary to induce favorable gene expression and engineered tissue formation is unknown...
October 7, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29027451/-enhancing-the-ability-of-autophagy-and-proliferation-of-bone-marrow-mesenchymal-stem-cells-by-interleukin-8-through-akt-stat3-pathway-in-hypoxic-environment
#3
Lei Shen, Shanqiang Zhang, Xiaodong Zhang, Yuting Zhang, Liping Xie, Yang Jiang, Yong Ma, Guofeng Li
To study the effects and mechanisms of interleukin-8 (IL-8) on the proliferation and autophagy of human bone marrow mesenchymal stem cells (hBMSC) under hypoxic condition. In the hypoxia model, we set the non-stimulated hBMSC as the hypoxia control group; the hBMSC stimulated by 100 μmol/L human IL-8 as the IL-8 group; the hBMSC stimulated by 50 μmol/L MK2206 (Akt protein inhibitor) and 100μmol/L IL-8 as the Akt inhibitor group; and the normal cultured hBMSC as the normal control group. The experiments of EdU cell proliferation and TUNEL apoptosis were respectively used to detect the number of positive cells that were labeled by EdU and apoptosis in each group, and Western blotting and ELISA were used respectively to detect the expression of autophagy protein (LC-3), Akt/STAT3 and other proteins in each group...
October 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28988007/chromosome-copy-number-variation-in-telomerized-human-bone-marrow-stromal-cells-insights-for-monitoring-safe-ex-vivo-expansion-of-adult-stem-cells
#4
Jorge S Burns, Linda Harkness, Abdullah Aldahmash, Laurent Gautier, Moustapha Kassem
Adult human bone marrow stromal cells (hBMSC) cultured for cell therapy require evaluation of potency and stability for safe use. Chromosomal aberrations upsetting genomic integrity in such cells have been contrastingly described as "Limited" or "Significant". Previously reported stepwise acquisition of a spontaneous neoplastic phenotype during three-year continuous culture of telomerized cells (hBMSC-TERT20) didn't alter a diploid karyotype measured by spectral karyotype analysis (SKY). Such screening may not adequately monitor abnormal and potentially tumorigenic hBMSC in clinical scenarios...
September 25, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28915498/tantalum-coated-pedicle-screws-enhance-implant-integration
#5
Liang-Yu Shi, An Wang, Fa-Zhi Zang, Jian-Xi Wang, Xian-Wei Pan, Hua-Jiang Chen
Because titanium alloy (Ti) has the natural advantage of a low elastic modulus, it has become the most commonly used material for the manufacturing of pedicle screws. However, its poor shear strength and osteogenic ability are undesirable properties. The superior osteoinductivity demonstrated by tantalum (Ta) in oral and maxillofacial surgery and joint surgery leads us to assume that the tantalum-coated pedicle screws may have better osteogenic properties and bone anchoring strength. To verify this hypothesis, MC3T3-E1 cells and human mesenchymal stem cells (hBMSCs) were seeded on the surface of Ta and Ti disks to compare the effects of two different metals on cell adhesion, proliferation, and differentiation...
September 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28883483/serpinb2-is-a-novel-tgf%C3%AE-responsive-lineage-fate-determinant-of-human-bone-marrow-stromal-cells
#6
Mona Elsafadi, Muthurangan Manikandan, Muhammad Atteya, Raed Abu Dawud, Sami Almalki, Zahid Ali Kaimkhani, Abdullah Aldahmash, Nehad M Alajez, Musaad Alfayez, Moustapha Kassem, Amer Mahmood
TGF-β1, a multifunctional regulator of cell growth and differentiation, is the most abundant bone matrix growth factor. During differentiation of human bone stromal cells (hBMSCs), which constitute bone marrow osteoblast (OS) and adipocyte (AD) progenitor cells, continuous TGF-β1 (10 ng/ml) treatment enhanced OS differentiation as evidenced by increased mineralised matrix production. Conversely, pulsed TGF-β1 administration during the commitment phase increased mature lipid-filled adipocyte numbers. Global gene expression analysis using DNA microarrays in hBMSCs treated with TGF-β1 identified 1587 up- and 1716 down-regulated genes in OS-induced, TGF-β1-treated compared to OS-induced hBMSCs (2...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28875591/osteogenic-potential-of-adipogenic-predifferentiated-human-bone-marrow-derived-multipotent-stromal-cells-for-bone-tissue-engineering
#7
Adrien Moya, Nathanaël Larochette, Marianne Bourguignon, Hanane El-Hafci, Esther Potier, Hervé Petite, Delphine Logeart-Avramoglou
In the present study, we evaluated the benefits of an adipogenic predifferentiation, the pathway most closely related to osteoblastogenesis, on the pro-osteogenic potential of human adult multipotent bone marrow stromal cells (hBMSCs), both in vitro and in vivo. Adipogenic differentiation of hBMSCs for 14 days resulted in a heterogeneous cell population from which the most adipogenic-committed cells were eliminated by their lack of re-adhesion ability. Our results provided evidence that the select adherent adipogenic differentiated hBMSCs (sAD+ cells) express a gene profile characteristic of both adipogenic and osteogenic lineages...
September 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28842963/metal-release-and-cell-biological-compatibility-of-beta-type-ti-40nb-containing-indium
#8
Stefan Pilz, Annett Gebert, Andrea Voss, Steffen Oswald, Markus Göttlicher, Ute Hempel, Jürgen Eckert, Marcus Rohnke, Jürgen Janek, Mariana Calin
Small indium (In) additions up to 5 wt % to the beta-type Ti-40Nb alloy effectively improve its mechanical biofunctionality. The impact on its biocompatibility is addressed in this work. Comparative electrochemical polarization studies and inductively coupled plasma optical emission spectrometry analyses were conducted in Tris-buffered saline (on the basis of 150 mM NaCl) with pH 7.6 and 2.0 at 310 ± 1 K with Ti-6Al-4V as reference. The metal ion releases from beta-type alloys were generally very low, for example, those of In(3+) ions from (Ti-40Nb)-4In specimens were below 6 × 10(-7) mmol/cm(2) ...
August 26, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28817816/exosomes-derived-from-human-bone-marrow-mesenchymal-stem-cells-promote-tumor-growth-through-hedgehog-signaling-pathway
#9
Jin Qi, Yali Zhou, Zuoyi Jiao, Xu Wang, Yang Zhao, Yangbin Li, Huijuan Chen, Luxi Yang, Hongwen Zhu, Yumin Li
BACKGROUND/AIMS: Mesenchymal stem/stromal cells (MSCs) are known to home to sites of tumor microenvironments where they participate in the formation of the tumor microenvironment and to interplay with tumor cells. However, the potential functional effects of MSCs on tumor cell growth are controversial. Here, we, from the view of bone marrow MSC-derived exosomes, study the molecular mechanism of MSCs on the growth of human osteosarcoma and human gastric cancer cells. METHODS: MSCs derived from human bone marrow (hBMSCs) were isolated and cultured in complete DMEM/F12 supplemented with 10% exosome-depleted fetal bovine serum and 1% penicillin-streptomycin, cell culture supernatants containing exosomes were harvested and exosome purification was performed by ultracentrifugation...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28698719/long-term-safety-of-transplanting-human-bone-marrow-stromal-cells-into-the-extravascular-spaces-of-the-choroid-of-rabbits
#10
Adi Tzameret, Sapir E Kalish, Ifat Sher, Lea Twito, Amilia Meir, Itay Levy, Shlomo Margel, Iris Moroz, Mordechai Rosner, Avraham J Treves, Arnon Nagler, Michael Belkin, Ygal Rotenstreich
Incurable neuroretinal degeneration diseases cause severe vision loss and blindness in millions of patients worldwide. In previous studies, we demonstrated that transplanting human bone marrow stromal cells (hBMSCs) in the extravascular spaces of the choroid (EVSC) of the Royal College of Surgeon rats ameliorated retinal degeneration for up to 5 months. Assessing the safety of hBMSC treatment and graft survival in a large animal is a crucial step before initiating clinical trials. Here, we transplanted hBMSCs into the EVSC compartment of New Zealand White rabbits...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28667751/a-supplement-free-osteoclast-osteoblast-co-culture-for-pre-clinical-application
#11
Sabine Schulze, Diana Wehrum, Peter Dieter, Ute Hempel
There is increasing demand for efficient and physiological in vitro cell culture systems suitable for testing new pharmaceutical drugs or for evaluating materials for tissue regeneration. In particular, co-cultures of two or more tissue-relevant cell types have the advantage to study the response of cells on diverse parameters in a more natural environment with respect to physiological complexity. We developed a direct bone cell co-culture system using human peripheral blood monocytes (hPBMC) and human bone marrow stromal cells (hBMSC) as osteoclast/osteoblast precursor cells, respectively, strictly avoiding external supplements for the induction of differentiation...
July 1, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28636926/enhanced-human-bone-marrow-mesenchymal-stromal-cell-adhesion-on-scaffolds-promotes-cell-survival-and-bone-formation
#12
Miryam Mebarki, Laura Coquelin, Pierre Layrolle, Séverine Battaglia, Marine Tossou, Philippe Hernigou, Hélène Rouard, Nathalie Chevallier
In order to induce an efficient bone formation with human bone marrow mesenchymal stromal cells (hBMSC) associated to a scaffold, it is crucial to determine the key points of the hBMSC action after in vivo transplantation as well as the appropriate features of a scaffold. To this aim we compared the hBMSC behavior when grafted onto two biomaterials allowing different bone potential in vivo. The cancellous devitalized Tutoplast®-processed bone (TPB) and the synthetic hydroxyapatite/β-tricalcium-phosphate (HA/βTCP) which give at 6weeks 100% and 50% of bone formation respectively...
September 1, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28622009/mir-139-5p-represses-bmsc-osteogenesis-via-targeting-wnt-%C3%AE-catenin-signaling-pathway
#13
Haitao Long, Buhua Sun, Liang Cheng, Shushan Zhao, Yong Zhu, Ruibo Zhao, Jianxi Zhu
Osteogenesis of mesenchymal stem cells (MSCs) has played a necessary role in the repair of bone. According to some reports, microRNAs participate in different physiological activity of the cells, including cell differentiation. This study investigated the function that miR-139-5p plays in the osteogenic differentiation of human bone marrow MSCs (hBMSCs). In addition to miR-139-5p, the effects of alkaline phosphatase (ALP), a membrane-bound metalloenzyme that is considered an early osteogenic differentiation marker, have also been investigated...
August 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28536312/effects-of-ginsenoside-rg1-loaded-alginate-chitosan-microspheres-on-human-bone-marrow-stromal-cells
#14
Yu-Hai Guo, Shuai Zhao, Yan-Xin Du, Qing-Jia Xing, Bo-Lai Chen, Chu-Qin Yu
The ginsenoside Rg1 is the most abundant compound of ginseng. Recent studies showed that Rg1 had neuroprotective effect on neuronal cells. This study was to prepare Rg1-loaded alginate-chitosan microspheres and research the effects of the microspheres on human bone marrow stromal cell (hBMSC). The alginate-chitosan microspheres were prepared by mechanical emulsification technique in combination with ion (Ca(2+)) and chitosan solidification. Subsequently, the microspheres were employed to load ginseng extracts Rg1...
May 23, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28533546/prostaglandin-e2-inhibits-matrix-mineralization-by-human-bone-marrow-stromal-cell-derived-osteoblasts-via-epac-dependent-camp-signaling
#15
Ali Mirsaidi, André N Tiaden, Peter J Richards
The osteoinductive properties of prostaglandin E2 (PGE2) and its signaling pathways have led to suggestions that it may serve as a potential therapeutic strategy for bone loss. However, the prominence of PGE2 as an inducer of bone formation is attributed primarily to findings from studies using rodent models. In the current study, we investigated the effects of PGE2 on human bone marrow stromal cell (hBMSC) lineage commitment and determined its mode of action. We demonstrated that PGE2 treatment of hBMSCs significantly altered the expression profile of several genes associated with osteoblast differentiation (RUNX2 and ALP) and maturation (BGLAP and MGP)...
May 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486747/a-simple-rocker-induced-mechanical-stimulus-upregulates-mineralization-by-human-osteoprogenitor-cells-in-fibrous-scaffolds
#16
Sasima Puwanun, Robin M Delaine Smith, Helen E Colley, Julian M Yates, Sheila MacNeil, Gwendolen C Reilly
Biodegradable electrospun polycaprolactone (PCL) scaffolds can be used to support bone-forming cells and could fill a thin bony defect, such as in cleft palate. Oscillatory fluid flow (OFF) has been shown to stimulate bone production in human progenitor cells in monolayer culture. The aim of this study was to examine whether bone matrix production by primary human mesenchymal stem cells from bone marrow (hBMSC) or jaw periosteal tissue (HJP) could be stimulated using OFF supplied by a standard see-saw rocker...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28453953/dual-functioning-peptides-discovered-by-phage-display-increase-the-magnitude-and-specificity-of-bmsc-attachment-to-mineralized-biomaterials
#17
Harsha Ramaraju, Sharon J Miller, David H Kohn
Design of biomaterials for cell-based therapies requires presentation of specific physical and chemical cues to cells, analogous to cues provided by native extracellular matrices (ECM). We previously identified a peptide sequence with high affinity towards apatite (VTKHLNQISQSY, VTK) using phage display. The aims of this study were to identify a human MSC-specific peptide sequence through phage display, combine it with the apatite-specific sequence, and verify the specificity of the combined dual-functioning peptide to both apatite and human bone marrow stromal cells...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28415545/engineering-bone-regeneration-with-novel-cell-laden-hydrogel-microfiber-injectable-calcium-phosphate-scaffold
#18
Yang Song, Chi Zhang, Ping Wang, Lin Wang, Chunyun Bao, Michael D Weir, Mark A Reynolds, Ke Ren, Liang Zhao, Hockin H K Xu
Cell-based tissue engineering is promising to create living functional tissues for bone regeneration. The implanted cells should be evenly distributed in the scaffold, be fast-released to the defect and maintain high viability in order to actively participate in the regenerative process. Herein, we report an injectable calcium phosphate cement (CPC) scaffold containing cell-encapsulating hydrogel microfibers with desirable degradability that could deliver cells in a timely manner and maintain cell viability...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28372263/extracellular-matrix-mimicking-scaffold-promotes-osteogenic-stem-cell-differentiation-a-new-approach-in%C3%A2-osteoporosis-research
#19
C W I Moll, T Schmiedinger, M A Moll, T Seppi, K Pfaller, M W Hess, K Gutleben, R A Lindtner, M Blauth, G Krumschnabel, H L Ebner
BACKGROUND: Osteoporosis is a common metabolic disease, with mesenchymal stem cells discussed to play an important role in its pathomechanism. For in vitro osteoporosis studies, selection of adequate culture conditions is mandatory so as to preserve cell properties as far as possible. A suitable cell culture surface would ideally provide reproducible experimental conditions by resembling those in-vivo. OBJECTIVE: Generating an improved growth surface for osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hBMSCs)...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28369869/connexin43-intercellular-communication-drives-the-early-differentiation-of-human-bone-marrow-stromal-cells-into-osteoblasts
#20
Julie Talbot, Régis Brion, Audrey Lamora, Mathilde Mullard, Sarah Morice, Dominique Heymann, Franck Verrecchia
Although it has been demonstrated that human bone marrow stromal cells (hBMSCs) express the ubiquitous connexin43 (Cx43) and form functional gap junctions, their role in the early differentiation of hBMSCs into osteoblasts remains poorly documented. Using in vitro assays, we show that Cx43 expression and gap junctional intercellular communication (GJIC) are increased during the differentiation of hBMSCs into osteoblasts, both at the protein and mRNA levels. Two independent procedures to reduce GJIC, a pharmacological approach with GJIC inhibitors (18α-glycyrrhetinic acid and Gap27 peptide) and a molecular approach using small interfering RNA against Cx43, demonstrated that the presence of Cx43 and functional junctional channels are essential to the ability of hBMSCs to differentiate into osteoblasts in vitro...
March 30, 2017: Journal of Cellular Physiology
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