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https://www.readbyqxmd.com/read/28698719/long-term-safety-of-transplanting-human-bone-marrow-stromal-cells-into-the-extravascular-spaces-of-the-choroid-of-rabbits
#1
Adi Tzameret, Sapir E Kalish, Ifat Sher, Lea Twito, Amilia Meir, Itay Levy, Shlomo Margel, Iris Moroz, Mordechai Rosner, Avraham J Treves, Arnon Nagler, Michael Belkin, Ygal Rotenstreich
Incurable neuroretinal degeneration diseases cause severe vision loss and blindness in millions of patients worldwide. In previous studies, we demonstrated that transplanting human bone marrow stromal cells (hBMSCs) in the extravascular spaces of the choroid (EVSC) of the Royal College of Surgeon rats ameliorated retinal degeneration for up to 5 months. Assessing the safety of hBMSC treatment and graft survival in a large animal is a crucial step before initiating clinical trials. Here, we transplanted hBMSCs into the EVSC compartment of New Zealand White rabbits...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28667751/a-supplement-free-osteoclast-osteoblast-co-culture-for-pre-clinical-application
#2
Sabine Schulze, Diana Wehrum, Peter Dieter, Ute Hempel
There is increasing demand for efficient and physiological in vitro cell culture systems suitable for testing new pharmaceutical drugs or for evaluating materials for tissue regeneration. In particular, co-cultures of two or more tissue-relevant cell types have the advantage to study the response of cells on diverse parameters in a more natural environment with respect to physiological complexity. We developed a direct bone cell co-culture system using human peripheral blood monocytes (hPBMC) and human bone marrow stromal cells (hBMSC) as osteoclast/osteoblast precursor cells, respectively, strictly avoiding external supplements for the induction of differentiation...
July 1, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28636926/enhanced-human-bone-marrow-mesenchymal-stromal-cell-adhesion-on-scaffolds-promotes-cell-survival-and-bone-formation
#3
Miryam Mebarki, Laura Coquelin, Pierre Layrolle, Séverine Battaglia, Marine Tossou, Philippe Hernigou, Hélène Rouard, Nathalie Chevallier
In order to induce an efficient bone formation with human bone marrow mesenchymal stromal cells (hBMSC) associated to a scaffold, it is crucial to determine the key points of the hBMSC action after in vivo transplantation as well as the appropriate features of a scaffold. To this aim we compared the hBMSC behavior when grafted onto two biomaterials allowing different bone potential in vivo. The cancellous devitalized Tutoplast®-processed bone (TPB) and the synthetic hydroxyapatite/β-tricalcium-phosphate (HA/βTCP) which give at 6 weeks 100% and 50% of bone formation respectively...
June 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28622009/mir-139-5p-represses-bmsc-osteogenesis-via-targeting-wnt-%C3%AE-catenin-signaling-pathway
#4
Haitao Long, Buhua Sun, Liang Cheng, Shushan Zhao, Yong Zhu, Ruibo Zhao, Jianxi Zhu
Osteogenesis of mesenchymal stem cells (MSCs) has played a necessary role in the repair of bone. According to some reports, microRNAs participate in different physiological activity of the cells, including cell differentiation. This study investigated the function that miR-139-5p plays in the osteogenic differentiation of human bone marrow MSCs (hBMSCs). In addition to miR-139-5p, the effects of alkaline phosphatase (ALP), a membrane-bound metalloenzyme that is considered an early osteogenic differentiation marker, have also been investigated...
June 16, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28536312/effects-of-ginsenoside-rg1-loaded-alginate-chitosan-microspheres-on-human-bone-marrow-stromal-cells
#5
Yu-Hai Guo, Shuai Zhao, Yan-Xin Du, Qing-Jia Xing, Bo-Lai Chen, Chu-Qin Yu
The ginsenoside Rg1 is the most abundant compound of ginseng. Recent studies showed that Rg1 had neuroprotective effect on neuronal cells. This study was to prepare Rg1-loaded alginate-chitosan microspheres and research the effects of the microspheres on human bone marrow stromal cell (hBMSC). The alginate-chitosan microspheres were prepared by mechanical emulsification technique in combination with ion (Ca(2+)) and chitosan solidification. Subsequently, the microspheres were employed to load ginseng extracts Rg1...
May 23, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28533546/prostaglandin-e2-inhibits-matrix-mineralization-by-human-bone-marrow-stromal-cell-derived-osteoblasts-via-epac-dependent-camp-signaling
#6
Ali Mirsaidi, André N Tiaden, Peter J Richards
The osteoinductive properties of prostaglandin E2 (PGE2) and its signaling pathways have led to suggestions that it may serve as a potential therapeutic strategy for bone loss. However, the prominence of PGE2 as an inducer of bone formation is attributed primarily to findings from studies using rodent models. In the current study, we investigated the effects of PGE2 on human bone marrow stromal cell (hBMSC) lineage commitment and determined its mode of action. We demonstrated that PGE2 treatment of hBMSCs significantly altered the expression profile of several genes associated with osteoblast differentiation (RUNX2 and ALP) and maturation (BGLAP and MGP)...
May 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486747/a-simple-rocker-induced-mechanical-stimulus-upregulates-mineralization-by-human-osteoprogenitor-cells-in-fibrous-scaffolds
#7
Sasima Puwanun, Robin M Delaine Smith, Helen E Colley, Julian M Yates, Sheila MacNeil, Gwendolen C Reilly
Biodegradable electrospun polycaprolactone (PCL) scaffolds can be used to support bone-forming cells and could fill a thin bony defect, such as in cleft palate. Oscillatory fluid flow (OFF) has been shown to stimulate bone production in human progenitor cells in monolayer culture. The aim of this study was to examine whether bone matrix production by primary human mesenchymal stem cells from bone marrow (hBMSC) or jaw periosteal tissue (HJP) could be stimulated using OFF supplied by a standard see-saw rocker...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28453953/dual-functioning-peptides-discovered-by-phage-display-increase-the-magnitude-and-specificity-of-bmsc-attachment-to-mineralized-biomaterials
#8
Harsha Ramaraju, Sharon J Miller, David H Kohn
Design of biomaterials for cell-based therapies requires presentation of specific physical and chemical cues to cells, analogous to cues provided by native extracellular matrices (ECM). We previously identified a peptide sequence with high affinity towards apatite (VTKHLNQISQSY, VTK) using phage display. The aims of this study were to identify a human MSC-specific peptide sequence through phage display, combine it with the apatite-specific sequence, and verify the specificity of the combined dual-functioning peptide to both apatite and human bone marrow stromal cells...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28415545/engineering-bone-regeneration-with-novel-cell-laden-hydrogel-microfiber-injectable-calcium-phosphate-scaffold
#9
Yang Song, Chi Zhang, Ping Wang, Lin Wang, Chunyun Bao, Michael D Weir, Mark A Reynolds, Ke Ren, Liang Zhao, Hockin H K Xu
Cell-based tissue engineering is promising to create living functional tissues for bone regeneration. The implanted cells should be evenly distributed in the scaffold, be fast-released to the defect and maintain high viability in order to actively participate in the regenerative process. Herein, we report an injectable calcium phosphate cement (CPC) scaffold containing cell-encapsulating hydrogel microfibers with desirable degradability that could deliver cells in a timely manner and maintain cell viability...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28372263/extracellular-matrix-mimicking-scaffold-promotes-osteogenic-stem-cell-differentiation-a-new-approach-in%C3%A2-osteoporosis-research
#10
C W I Moll, T Schmiedinger, M A Moll, T Seppi, K Pfaller, M W Hess, K Gutleben, R A Lindtner, M Blauth, G Krumschnabel, H L Ebner
BACKGROUND: Osteoporosis is a common metabolic disease, with mesenchymal stem cells discussed to play an important role in its pathomechanism. For in vitro osteoporosis studies, selection of adequate culture conditions is mandatory so as to preserve cell properties as far as possible. A suitable cell culture surface would ideally provide reproducible experimental conditions by resembling those in-vivo. OBJECTIVE: Generating an improved growth surface for osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hBMSCs)...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28369869/connexin43-intercellular-communication-drives-the-early-differentiation-of-human-bone-marrow-stromal-cells-into-osteoblasts
#11
Julie Talbot, Régis Brion, Audrey Lamora, Mathilde Mullard, Sarah Morice, Dominique Heymann, Franck Verrecchia
Although it has been demonstrated that human bone marrow stromal cells (hBMSCs) express the ubiquitous connexin43 (Cx43) and form functional gap junctions, their role in the early differentiation of hBMSCs into osteoblasts remains poorly documented. Using in vitro assays, we show that Cx43 expression and gap junctional intercellular communication (GJIC) are increased during the differentiation of hBMSCs into osteoblasts, both at the protein and mRNA levels. Two independent procedures to reduce GJIC, a pharmacological approach with GJIC inhibitors (18α-glycyrrhetinic acid and Gap27 peptide) and a molecular approach using small interfering RNA against Cx43, demonstrated that the presence of Cx43 and functional junctional channels are essential to the ability of hBMSCs to differentiate into osteoblasts in vitro...
March 30, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28340487/microrna-4739-regulates-osteogenic-and-adipocytic-differentiation-of-immortalized-human-bone-marrow-stromal-cells-via-targeting-lrp3
#12
Mona Elsafadi, Muthurangan Manikandan, Nehad M Alajez, Rimi Hamam, Raed Abu Dawud, Abdullah Aldahmash, Zafar Iqbal, Musaad Alfayez, Moustapha Kassem, Amer Mahmood
Understanding the regulatory networks underlying lineage differentiation and fate determination of human bone marrow stromal cells (hBMSC) is a prerequisite for their therapeutic use. The goal of the current study was to unravel the novel role of the low-density lipoprotein receptor-related protein 3 (LRP3) in regulating the osteogenic and adipogenic differentiation of immortalized hBMSCs. Gene expression profiling revealed significantly higher LRP3 levels in the highly osteogenic hBMSC clone imCL1 than in the less osteogenic clone imCL2, as well as a significant upregulation of LRP3 during the osteogenic induction of the imCL1 clone...
April 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28286408/antinociceptive-effect-of-intrathecal-injection-of-genetically-engineered-human-bone-marrow-stem-cells-expressing-the-human-proenkephalin-gene-in-a-rat-model-of-bone-cancer-pain
#13
Yi Sun, Yuke Tian, Haifeng Li, Dengwen Zhang, Qiang Sun
Background. This study aimed to investigate the use of human bone marrow mesenchymal stem cells (hBMSCs) genetically engineered with the human proenkephalin (hPPE) gene to treat bone cancer pain (BCP) in a rat model. Methods. Primary cultured hBMSCs were passaged and modified with hPPE, and the cell suspensions (6 × 10(6)) were then intrathecally injected into a rat model of BCP. Paw mechanical withdrawal threshold (PMWT) was measured before and after BCP. The effects of hPPE gene transfer on hBMSC bioactivity were analyzed in vitro and in vivo...
2017: Pain Research & Management: the Journal of the Canadian Pain Society
https://www.readbyqxmd.com/read/28211911/three-dimensional-printing-of-calcium-sulfate-and-mesoporous-bioactive-glass-scaffolds-for-improving-bone-regeneration-in-vitro-and-in-vivo
#14
Xin Qi, Peng Pei, Min Zhu, Xiaoyu Du, Chen Xin, Shichang Zhao, Xiaolin Li, Yufang Zhu
In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. In the field of bone tissue engineering, three-dimensional (3D) scaffolds are promising for the treatment of bone defects. In this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) scaffolds were successfully fabricated using a 3D printing technique, which had a regular and uniform square macroporous structure, high porosity and excellent apatite mineralization ability...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28181209/heparin-modification-of-a-biomimetic-bone-matrix-modulates-osteogenic-and-angiogenic-cell-response-in-vitro
#15
M Quade, S Knaack, D Weber, U König, B Paul, P Simon, A Rösen-Wolff, R Schwartz-Albiez, M Gelinsky, A Lode
In this study, the effect of heparin-modified collagen type I/hydroxyapatite (HA) nanocomposites on key processes of bone regeneration - osteogenesis and angiogenesis - was characterised in vitro. Two approaches were applied for heparin modification: it was either integrated during material synthesis (in situ) or added to the porous scaffolds after their fabrication (post). Cultivation of human bone marrow-derived stromal cells (hBMSC), in heparin-modified versus heparin-free scaffolds, revealed a positive effect of the heparin modification on their proliferation and osteogenic differentiation...
February 9, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28033461/accelerated-bone-regeneration-by-two-photon-photoactivated-carbon-nitride-nanosheets
#16
Jitendra N Tiwari, Young-Kyo Seo, Taeseung Yoon, Wang Geun Lee, Woo Jong Cho, Muhammad Yousuf, Ahmad M Harzandi, Du-Seok Kang, Kwang-Youn Kim, Pann-Ghill Suh, Kwang S Kim
Human bone marrow-derived mesenchymal stem cells (hBMSCs) present promising opportunities for therapeutic medicine. Carbon derivatives showed only marginal enhancement in stem cell differentiation toward bone formation. Here we report that red-light absorbing carbon nitride (C3N4) sheets lead to remarkable proliferation and osteogenic differentiation by runt-related transcription factor 2 (Runx2) activation, a key transcription factor associated with osteoblast differentiation. Accordingly, highly effective hBMSCs-driven mice bone regeneration under red light is achieved (91% recovery after 4 weeks compared to 36% recovery in the standard control group in phosphate-buffered saline without red light)...
January 3, 2017: ACS Nano
https://www.readbyqxmd.com/read/28024469/-role-of-non-classical-nf-%C3%AE%C2%BAb-signaling-pathway-in-b-acute-lymphoblastic-leukemia
#17
Xiao-Lei Liu, Yi Cai, Bao-Jing Li, Wei-Bo Huang
OBJECTIVE: To study the role of non-classical NF-κB signaling pathway in B acute lymphoblastic leukemia (B-ALL). METHODS: The bone marrow samples from 48 patients with B-ALL were collected from March 2015 to March 2016. The real-time quantitative RT-PCR was used for determing mRNA expression levels of NF-κB family members; the NF-κB DNA binding activity in B-ALL cell nucleus was analyzed by ELISA; the apoptosis rate of B-ALL cells alone or co-cultured with bone marrow stromal cells (hBMSC) was determined by flow cytometry...
December 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27986563/role-and-mechanism-of-pten-in-adiponectin-induced-osteogenesis-in-human-bone-marrow-mesenchymal-stem-cells
#18
Xuhong Liu, Tong Chen, Yuwei Wu, Zhihui Tang
Human bone marrow-derived stromal cells (hBMSC) are multi-potent stem cells that can differentiate into osteogenic and adipogenic lineages. Adiponectin (APN) is an adipocyte-derived hormone that modulates a series of metabolic processes. Recent studies revealed a relationship between APN and bone regeneration, though the underlying mechanism was not fully examined. Phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a tumor suppressor and a therapeutic target for the metabolic syndrome. Its deletion mutants increase osteoblast activity and bone mineral density...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27935982/a-cost-effective-method-to-assemble-biomimetic-3d-cell-culture-platforms
#19
Sabreen Khalil, Nagwa El-Badri, Mohamed El-Mokhtaar, Saif Al-Mofty, Mohamed Farghaly, Radwa Ayman, Dina Habib, Noha Mousa
METHODS: We utilized the hAM to provide the biological and the three dimensional (3D) topographic components of the prototype. The 3D nano-roughness of the hAM was characterized using surface electron microscopy and surface image analysis (ImageJ and SurfaceJ). We developed additional macro-scale and micro-scale versions of the platform which provided additional shear stress factors to simulate the fluid dynamics of the in vivo extracellular fluids. RESULTS: Three models of varying complexities of the prototype were assembled...
2016: PloS One
https://www.readbyqxmd.com/read/27893751/25-hydroxy-and-1%C3%AE-25-dihydroxycholecalciferol-have-greater-potencies-than-25-hydroxy-and-1%C3%AE-25-dihydroxyergocalciferol-in-modulating-cultured-human-and-mouse-osteoblast-activities
#20
COMPARATIVE STUDY
Allahdad Zarei, Philippa A Hulley, Afsie Sabokbar, M Kassim Javaid, Alireza Morovat
Despite differences in the phamacokinetics of 25-hydroxycholecalciferol (25(OH)D3) and 25-hydroxyergocalciferol (25(OH)D2) in man, the effects of these and their 1α-hydroxylated forms (1,25(OH)2D3 and 1,25(OH)2D2) on cellular activity of vitamin D-responsive cells have hardly been compared. We studied differences in the effects of these metabolites on cell number, gene transcription, protein expression and mineralisation of cultured human bone marrow-derived stromal cells (hBMSC) and rapidly mineralising mouse 2T3 osteoblasts...
2016: PloS One
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