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https://www.readbyqxmd.com/read/29454232/effects-of-mine-tailing-and-mixed-contamination-on-metals-trace-elements-accumulation-and-histopathology-of-the-chub-squalius-cephalus-tissues-evidence-from-three-differently-contaminated-sites-in-serbia
#1
Božidar Rašković, Vesna Poleksić, Stefan Skorić, Katarina Jovičić, Slađana Spasić, Aleksandar Hegediš, Nebojša Vasić, Mirjana Lenhardt
Chub (Squalius cephalus) specimens were collected in Korenita River seven months after spillover from the waste water of antimony mine tailing pond and compared with chub living in Kruščica reservoir (intended for water supply) and Međuvršje reservoir (influenced by intense emission of industrial, urban and rural wastewater). Concentrations of 15 elements (Al, As, B, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Si, Sr, Zn) were determined in muscle, liver and gills of chub by inductively coupled plasma optical spectrometry (ICP-OES) and histopathological alterations in liver and gills were assessed...
February 14, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29454191/show-me-the-way-rust-effector-targets-in-heterologous-plant-systems
#2
REVIEW
Cécile Lorrain, Benjamin Petre, Sébastien Duplessis
For years, the study of rust fungal effectors has been impeded by the lack of molecular genetic tools in rust pathosystems. The recent use of heterologous plants to perform effector screens (effectoromics)-including effector localisation (cellular targets) and protein interactors (molecular targets) in plant cells-has changed the game. These screens revealed that many candidate effectors from various rust fungi target specific plant cell compartments, including chloroplasts, and associate with specific plant protein complexes...
February 14, 2018: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29452850/evaluation-of-dot-blot-test-for-serological-diagnosis-of-bovine-brucellosis
#3
Carla Resende Bastos, Luis Antonio Mathias, Márcia Mariza Gomes Jusi, Renata Ferreira Dos Santos, Glaucenyra Cecília Pinheiro da Silva, Marcos Rogério André, Rosangela Zacarias Machado, Karina Paes Bürger
The objective of this study was to standardize and validate the dot-blot test for the serological diagnosis of bovine brucellosis, compare the results with those found in the 2-mercaptoethanol (2-ME) and complement fixation test (CF), and estimate the relative sensitivity and specificity of the dot-blot compared to these tests. Fifty bovine blood serum samples were used for the test standardization, and 1315 samples were used for evaluation and comparison between the tests; the results were compared using the Kappa indicator...
February 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29452227/age-and-experience-dependent-changes-in-egr-1-expression-during-the-ontogeny-of-the-context-preexposure-facilitation-effect-cpfe
#4
P A Robinson-Drummer, T Chakraborty, N A Heroux, J B Rosen, M E Stanton
The context preexposure facilitation effect (CPFE) is a variant of contextual fear conditioning in which acquisition of the contextual representation and association of the retrieved contextual memory with an immediate foots-hock are separated by 24hrs. During the CPFE, learning- related expression patterns of the early growth response -1 gene (Egr-1) vary based on training phase and brain sub-region in adult and adolescent rats (Asok, Schreiber, Jablonski, Rosen, & Stanton, 2013; Shreiber et al., 2014; Chakraborty, Asok, Stanton, & Rosen, 2016)...
February 13, 2018: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/29451985/a-comparison-of-barriers-to-accessing-services-for-mental-and-physical-health-conditions-in-a-sample-of-rural-australian-adults
#5
Kate Fennell, Melissa Hull, Martin Jones, James Dollman
INTRODUCTION: The prevalence of chronic disease, mortality and suicide rates is higher in rural Australia than in urban centres. Understanding rural Australians' barriers to accessing health services requires urgent attention. The purpose of this study was to compare barriers to help-seeking for physical and mental health issues among rural South Australian adults. METHODS: A total of 409 people from three rural and remote regions in South Australia completed a computer-assisted telephone interview...
February 2018: Rural and Remote Health
https://www.readbyqxmd.com/read/29451574/diastereoselective-synthesis-structure-and-reactivity-studies-of-ferrocenyloxazoline-gold-i-and-gold-ii-complexes
#6
Julia Holz, Marta Ayerbe García, Wolfgang Frey, Felix Krupp, René Peters
In the last few decades, gold complexes have demonstrated huge potential for soft Lewis acid catalysis. Despite the intensive research on Au complexes and planar chiral metallacycles, enantiopure ferrocenylgold complexes have - surprisingly - not been reported until the studies presented in this article. Herein, we report the asymmetric synthesis of planar chiral ferrocenyl Au(i) complexes. These dinuclear species form helically chiral ten-membered (NCCCAu) 2 rings stabilized by aurophilic interactions. In supramolecular solid state structures, linear, zigzag or helical Au(i) wires with regular AuAu separations were observed...
February 16, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29450955/n-functionalized-ferrocenes-subvalent-group-xiv-element-chlorides-and-an-tbutyl-lithium-induced-c%C3%A2-c-bond-cleavage-under-mild-conditions
#7
Bastian Nayyar, Hazem Alnasr, Wolf Hiller, Klaus Jurkschat
The ferrocene derivative η5-CpFeη5-C5H3-1-(ArNCH)-2-(CH2NMe2) (1, Ar = 2,6-iPr2C6H3)) reacts diastereoselectively with LiR under carbolithiation and subsequent hydrolysis to give η5-CpFeη5-C5H3-1-(ArHNCHR)-2-(CH2NMe2) (3, R = tBu; 4, R = Ph; 5, R = Me) in high yields. For R = tBu the organolithium derivative η5-CpFeη5-C5H3-1-(ArLiNCHR)-2-(CH2NMe2) (2) was isolated. Compound 2 reacts with GeCl2∙dioxane and SnCl2, respectively, giving the metallylene amide chlorides η5-CpFeη5-C5H3-1-(ArMNCHtBu)-2-(CH2NMe2) (6, M = GeCl; 7, M = SnCl) containing each three stereogenic centres...
February 16, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29450815/factors-affecting-reproductive-performance-in-dromedary-camel-herds-in-saudi-arabia
#8
Ahmed Ali, Derar Derar, Abdulhadi Alsharari, Assaf Alsharari, Rashid Khalil, Tariq I Almundarij, Yaser Alboti, Fahd Al-Sobayil
A survey of 7122 dromedary camels in 115 herds in Saudi Arabia was used to estimate the effects of herd size (HZ; < 25 vs. 25-49 vs. 50-100 vs. > 100 camels), herder/camels ratio (H/C; 1:< 25 vs. 1:25-50 vs. 1:> 50), manager experience (ME; < 5 vs. 5-10 vs. > 10 years), male/females ratio (M/F), housing system (HS; free vs. closed vs. mixed), length of the breeding season (winter vs. winter and spring vs. fall, winter and spring), age at first mating (3 vs. > 3 years), and time of mating after parturition (≤ 3 vs...
February 15, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29449592/first-evidence-for-a-multienzyme-complex-of-lipid-biosynthesis-pathway-enzymes-in-cunninghamella-bainieri
#9
Shuwahida Shuib, Izyanti Ibrahim, Mukram Mohamed Mackeen, Colin Ratledge, Aidil Abdul Hamid
Malic enzyme (ME) plays a vital role in determining the extent of lipid accumulation in oleaginous fungi being the major provider of NADPH for the activity of fatty acid synthase (FAS). We report here the first direct evidence of the existence of a lipogenic multienzyme complex (the lipid metabolon) involving ME, FAS, ATP: citrate lyase (ACL), acetyl-CoA carboxylase (ACC), pyruvate carboxylase (PC) and malate dehydrogenase (MDH) in Cunninghamella bainieri 2A1. Cell-free extracts prepared from cells taken in both growth and lipid accumulation phases were prepared by protoplasting and subjected to Blue Native (BN)-PAGE coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29449589/loss-of-the-homeostatic-protein-bpifa1-leads-to-exacerbation-of-otitis-media-severity-in-the-junbo-mouse-model
#10
Apoorva Mulay, Derek W Hood, Debbie Williams, Catherine Russell, Steve D M Brown, Lynne Bingle, Michael Cheeseman, Colin D Bingle
Otitis Media (OM) is characterized by epithelial abnormalities and defects in innate immunity in the middle ear (ME). Although, BPIFA1, a member of the BPI fold containing family of putative innate defence proteins is abundantly expressed by the ME epithelium and SNPs in Bpifa1 have been associated with OM susceptibility, its role in the ME is not well characterized. We investigated the role of BPIFA1 in protection of the ME and the development of OM using murine models. Loss of Bpifa1 did not lead to OM development...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29448798/effect-of-alkaline-metal-cations-on-the-ionic-structure-of-cryolite-melts-ab-initio-npt-md-study
#11
Tomáš Bučko, František Šimko
Ab initio molecular dynamics simulations in an NpT ensemble have been performed to study the role of alkaline metal cations (Me = Li, Na, K, Rb) on the structure and vibrational properties of melts of Me-cryolites (Me 3 AlF 6 ) at T = 1300 K. In all melts examined in this work, the species AlF 5 2- has been found to be formed at the highest abundance [from 58% (Li) to 70% (Na)] among the Al-containing anionic clusters. The concentration of clusters AlF 4 - increases with the size of cations while that of anions AlF 6 3- follows the opposite trend and it becomes negligible in the melts of the K- and Rb-cryolites...
February 14, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29448017/fetal-bovine-serum-free-cryopreservation-methods-for-clinical-banking-of-human-adipose-derived-stem-cells
#12
Seah Park, Dong Ryul Lee, Ji Sun Nam, Chul Woo Ahn, Haekwon Kim
The use of fetal bovine serum (FBS) as a cryopreservation supplement is not suitable for the banking of mesenchymal stem cells (MSCs) due to the risk of transmission of disease as well as xenogeneic immune reactions in the transplanted host. Here, we investigated if human serum albumin (HSA), human serum (HS), or knockout serum replacement (KSR) can replace FBS for the cryopreservation of MSCs. In addition, we examined the characteristics of MSCs after multiple rounds of cryopreservation. Human adipose-derived stem cells (ASCs) cryopreserved with three FBS replacements, 9% HSA, 90% HS, or 90% KSR, in combination with 10% dimethyl sulfoxide (Me 2 SO) maintained stem cell properties including growth, immunophenotypes, gene expression patterns, and the potential to differentiate into adipogenic, osteogenic, and chondrogenic lineages, similar to ASCs frozen with FBS...
February 12, 2018: Cryobiology
https://www.readbyqxmd.com/read/29447951/suction-electrode-recording-in-locus-coeruleus-of-newborn-rat-brain-slices-reveals-network-bursting-comprising-summated-non-synchronous-spiking
#13
Vladimir Rancic, Bijal Rawal, Bogdan Panaitescu, Araya Ruangkittisakul, Klaus Ballanyi
The brainstem locus coeruleus (LC) controling behaviors like arousal, sleep, breathing, pain or opioid withdrawal is an established model for spontaneous action potential synchronization. Such synchronous 'spiking' might produce an extracellular field potential (FP) which is a crucial tool for neural network analyses. We found using ≥10 μm tip diameter suction electrodes in newborn rat brainstem slices that the LC generates at ∼1 Hz a robust rhythmic FP (rFP). During distinct rFP phases, LC neurons discharge with a jitter of ±33 ms single spikes that summate to a ∼200 ms-lasting population burst...
February 12, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29447089/aflibercept-versus-bevacizumab-and-or-ranibizumab-for-recurrent-macular-edema-secondary-to-central-retinal-vein-occlusion
#14
Cem Ozgonul, Vaidehi S Dedania, Cagri G Besirli
PURPOSE: To compare functional and anatomic outcomes of treatment with intravitreal aflibercept versus bevacizumab and/or ranibizumab in patients with recurrent macular edema (ME) secondary to central retinal vein occlusion (CRVO). METHODS: Retrospective, comparative case series of patients with recurrent ME in the setting of CRVO. Patients with recurrent ME received treatment with aflibercept (Group 1, G1) or bevacizumab and/or ranibizumab (Group 2, G2). Primary outcome measures were best-corrected visual acuity (BCVA) and central foveal thickness (CFT)...
February 15, 2018: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29446925/visible-light-driven-photosystems-using-heteroleptic-cu-i-photosensitizers-and-rh-iii-catalysts-to-produce-h-2
#15
Bradley J McCullough, Bertrand J Neyhouse, Briana R Schrage, Demi T Reed, Allen J Osinski, Christopher J Ziegler, Travis A White
The synthesis of two new heteroleptic Cu(I) photosensitizers (PS), [Cu(Xantphos)(NN)]PF 6 (NN = biq = 2,2'-biquinoline, dmebiq = 2,2'-biquinoline-4,4'-dimethyl ester; Xantphos = 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene), along with the associated structural, photophysical, and electrochemical properties, are described. The biquinoline diimine ligand extends the PS light absorbing properties into the visible with a maximum absorption at 455 and 505 nm for NN = biq and dmebiq, respectively, in CH 2 Cl 2 solvent...
February 15, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29446830/prior-ingestion-of-exogenous-ketone-monoester-attenuates-the-glycemic-response-to-an-oral-glucose-tolerance-test-in-healthy-young-individuals
#16
Etienne Myette-Côté, Helena Neudorf, Hossein Rafiei, Kieran Clarke, Jonathan Peter Little
The main objectives of this study were threefold: (1) To determine whether acute ingestion of K me ; (R)-3-hydroxybutyl (R)-3-hydroxybutyrate impacts plasma glucose levels during a standardized oral glucose tolerance test (OGTT). (2) To compare changes in insulin concentrations and estimates of insulin sensitivity after acute K me supplementation. Twenty healthy participants (n = 10 males/females) aged between 18-35 years took part in a randomized crossover study. After an overnight fast, participants consumed a K me supplement (ΔG®; 0...
February 15, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29446421/a-monoanionic-nnnn-type-macrocyclic-ligand-for-electropositive-metal-centers
#17
Debabrata Mukherjee, Jun Okuda
The coordination of a mono(amido) triamine macrocyclic ligand Me 3 TACD derived from cyclen (1,4,7,10-tetraazacyclododecane or [12]aneN 4 ) toward electropositive metal centers is surveyed. This NNNN-type monoanionic macrocyclic ligand is capable of stabilizing a variety of metal coordination environments that are commonly not amenable to stable coordination spheres. Metal complexes supported by Me 3 TACD include hydrides, hydrocarbyls, silyls, and amides. Protection of one hemisphere of a large metal center allows, as in the case of metallocene scaffolds for early transition metals, deaggregation and control of reactivity...
February 15, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29446236/help-me-if-you-can-psychological-distance-and-help-seeking-intentions-in-employee-supervisor-relations
#18
Izhak Berkovich, Ori Eyal
Social support at work is considered useful in treating job-related stress, and supervisors' emotional support has been found to be the most effective source of support at work. But an understanding of what elements make employees use supervisors as a source of emotional support is lacking. The present qualitative study included in-depth interviews with 24 teachers and 12 principals and a focus group with 12 school counsellors. The findings pointed at 2 groups of determinants of subordinates' intentions of asking socioemotional help from supervisors...
February 15, 2018: Stress and Health: Journal of the International Society for the Investigation of Stress
https://www.readbyqxmd.com/read/29446041/characteristic-findings-of-high-grade-cervical-intraepithelial-neoplasia-or-more-on-magnifying-endoscopy-with-narrow-band-imaging
#19
Kunihisa Uchita, Kenji Kanenishi, Koki Hirano, Hideki Kobara, Noriko Nishiyama, Ai Kawada, Shintaro Fujihara, Emi Ibuki, Reiji Haba, Yohei Takahashi, Yuka Kai, Kenji Yorita, Hirohito Mori, Jun Kunikata, Naoki Nishimoto, Toshiyuki Hata, Tsutomu Masaki
BACKGROUND: Colposcopy, which is a standard modality for diagnosing cervical intraepithelial neoplasia (CIN), can have limited accuracy owing to poor visibility. Flexible magnifying endoscopy with narrow band imaging (ME-NBI) has excellent diagnostic accuracy for early gastrointestinal neoplasms and is expected to be highly useful for CIN diagnosis. This study aimed to determine the characteristic findings and evaluate the diagnostic ability of ME-NBI for lesions ≥ CIN 3. METHODS: A well-designed prospective diagnostic case series conducted at multiple tertiary-care centers...
February 14, 2018: International Journal of Clinical Oncology
https://www.readbyqxmd.com/read/29445968/arthroscopic-direct-meniscal-extrusion-reduction-surgical-tips-to-reduce-persistent-meniscal-extrusion-in-meniscal-root-repair
#20
Bancha Chernchujit, Renaldi Prasetia
Meniscal extrusion (ME) is defined as extension of the meniscal margin by at least 3 mm beyond the tibial margin. The main purpose of meniscal root repair is to restore the anatomy and function of the meniscus. Therefore, the reduction in the ME is one of the important objective outcomes. Nevertheless, the reduced meniscal extrusion was obtained in limited patients after meniscal root repair. This technical note described the arthroscopic direct meniscal extrusion reduction as surgical tips to reduce persistent meniscal extrusion in posterior meniscal root repair...
February 14, 2018: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
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