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https://www.readbyqxmd.com/read/29146943/cryopreservation-of-human-mesenchymal-stem-cells-in-an-allogeneic-bioscaffold-based-on-platelet-rich-plasma-and-synovial-fluid
#1
Haritz Gurruchaga, Laura Saenz Del Burgo, Ane Garate, Diego Delgado, Pello Sanchez, Gorka Orive, Jesús Ciriza, Mikel Sanchez, Jose Luis Pedraz
Transplantation of mesenchymal stem cells (MSCs) has emerged as an alternative strategy to treat knee osteoarthritis. In this context, MSCs derived from synovial fluid could provide higher chondrogenic and cartilage regeneration, presenting synovial fluid as an appropriate MSCs source. An allogeneic and biomimetic bioscaffold composed of Platelet Rich Plasma and synovial fluid that preserve and mimics the natural environment of MSCs isolated from knee has also been developed. We have optimized the cryopreservation of knee-isolated MSCs embedded within the aforementioned biomimetic scaffold, in order to create a reserve of young autologous embedded knee MSCs for future clinical applications...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29145993/an-absorbable-hydrogel-spray-reduces-postoperative-mediastinal-adhesions-after-congenital-heart-surgery
#2
Nahidh Hasaniya, Anees Razzouk, Jennifer Newcombe, Dana Hassneiah, Jessica Heimes, Joshua Gysbers, Timothy Martens, Leonard Bailey
BACKGROUND: Adhesions encountered during reoperative cardiac surgery can prolong operative time and increase operative risk. The purpose of this clinical study was to investigate the antiadhesion property of a synthetic bioabsorbable polymer spray after cardiac reoperations in infants. METHODS: A prospective randomized double-blinded study was designed. Forty infants requiring staged cardiac operations were randomly allocated to a study group (n = 20) or a control group (n = 20)...
November 14, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29144152/new-psychometric-data-from-the-spanish-versions-of-the-glasgow-dyspepsia-severity-score-and-the-dyspepsia-related-health-scale-measures
#3
Miren Orive, Ane Antón-Ladislao, Nerea González, Begoña Matellanes, Jesús Ángel Padierna, José Luis Cabriada, Aitor Orive, Víctor Manuel Orive, José María Quintana
BACKGROUND AND OBJECTIVE: There are no structural abnormalities in functional dyspepsia, therefore it is essential to have a viable questionnaire to measure treatment outcome according to patient perception. The aim of the study was to extensively document psychometric characteristics of the Glasgow Dyspepsia Severity Score and the Dyspepsia-Related Health Scale that are currently available in Spanish. METHODS: Patients with functional dyspepsia (n = 158) were recruited from a randomized trial that assessed standard vs...
November 16, 2017: Revista Española de Enfermedades Digestivas
https://www.readbyqxmd.com/read/29142222/human-atg3-binding-to-lipid-bilayers-role-of-lipid-geometry-and-electric-charge
#4
Javier H Hervás, Ane Landajuela, Zuriñe Antón, Anna V Shnyrova, Felix M Goñi, Alicia Alonso
Specific protein-lipid interactions lead to a gradual recruitment of AuTophaGy-related (ATG) proteins to the nascent membrane during autophagosome (AP) formation. ATG3, a key protein in the movement of LC3 towards the isolation membrane, has been proposed to facilitate LC3/GABARAP lipidation in highly curved membranes. In this work we have performed a biophysical study of human ATG3 interaction with membranes containing phosphatidylethanolamine, phosphatidylcholine and anionic phospholipids. We have found that ATG3 interacts more strongly with negatively-charged phospholipid vesicles or nanotubes than with electrically neutral model membranes, cone-shaped anionic phospholipids (cardiolipin and phosphatidic acid) being particularly active in promoting binding...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29132698/risk-factor-analysis-for-intraoperative-extracorporeal-membrane-oxygenation-weaning-failure-after-lung-transplantation
#5
Kyoung Shik Narm, Seokkee Lee, Jee Won Suh, Anes Kim, Jin Gu Lee, Moo Suk Park, Song Yee Kim, Joo Han Song, Su Jin Jeong, Hyo Chae Paik
BACKGROUND: Extracorporeal membrane oxygenation (ECMO) has been widely used for hemodynamic support during lung transplantation (LTx). We evaluated the risk factors associated with failure of weaning from ECMO in the operating room during LTx. METHODS: We retrospectively reviewed 74 consecutive patients who had undergone LTx from March 2013 to February 2016. Patients who underwent single LTx, multiorgan transplantation, and LTx for pulmonary hypertension were excluded...
November 11, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29131567/highly-efficient-antibacterial-surfaces-based-on-bacterial-cell-size-selective-microporous-supports
#6
Nelson Vargas Alfredo, Ana Coquillat, Enrique Martinez-Campos, Ane Dorronsoro, Aitziber L Cortajarena, Adolfo Del Campo, Juan Rodriguez-Hernandez
We report on the fabrication of efficient antibacterial substrates selective for bacteria, i.e. non-cytotoxic against mammalian cells. The strategy proposed is based on the different size of bacteria (1-4 m) in comparison with mammalian cells (above 20 µm) that permit the bacteria to enter in contact with the inner part of micrometer size pores where the antimicrobial functionality are placed. On the contrary, mammalian cells, larger in terms of size, remain at the top surface thus reducing adverse cytotoxic effects and improving the biocompatibility of the substrates...
November 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29130523/subclinical-thinning-of-macular-ganglion-cell-layer-in-leber-optic-neuropathy-carriers
#7
Julio González-Martín-Moro, Maria Castro-Rebollo, Inés Contreras, Ane Pérez-Sarriegui
No abstract text is available yet for this article.
November 12, 2017: Clinical & Experimental Optometry: Journal of the Australian Optometrical Association
https://www.readbyqxmd.com/read/29120089/dna-damage-in-arctic-seabirds-baseline-sensitivity-to-a-genotoxic-stressor-and-association-to-organohalogen-contaminants
#8
Ane Haarr, Ketil Hylland, Norith Eckbo, Geir Wing Gabrielsen, Dorte Herzke, Jan Ove Bustnes, Pierre Blevin, Olivier Chastel, Børge Moe, Sveinn Are Hanssen, Kjetil Sagerup, Katrine Borgå
Environmental contaminants are found throughout Arctic marine ecosystems, and their presence in seabirds has been associated with toxicological responses. However, there are few studies of genotoxicity in Arctic avian wildlife. The purpose of the present study was to quantify DNA damage in lymphocytes of selected seabird species and to examine whether accumulation of organohalogen contaminants ( OHCs) affects DNA damage. Blood was sampled from common eider (Somateria mollissima), black guillemot (Cepphus grylle), black-legged kittiwake (Rissa tridactyla), glaucous gull (Larus hyperboreus), arctic skua (Stercorarius parasiticus), and great skua (Stercorarius skua) in Kongsfjorden, Svalbard...
November 9, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29118783/cerebrospinal-fluid-phosphate-in-delirium-after-hip-fracture
#9
Ane-Victoria Idland, Torgeir Bruun Wyller, Randi Støen, Gry Torsæter Dahl, Frede Frihagen, Anne Brækhus, Bjørnar Hassel, Leiv Otto Watne
Aims: Phosphate is essential for neuronal activity. We aimed to investigate whether delirium is associated with altered phosphate concentrations in cerebrospinal fluid (CSF) and serum. Methods: Seventy-seven patients with hip fracture were assessed for delirium before and after acute surgery. Prefracture dementia was diagnosed by an expert panel. Phosphate was measured in CSF obtained immediately before spinal anesthesia (n = 77) and in serum (n = 47). CSF from 23 cognitively healthy elderly patients undergoing spinal anesthesia was also analyzed...
September 2017: Dementia and Geriatric Cognitive Disorders Extra
https://www.readbyqxmd.com/read/29117468/zoonotic-helminth-exposure-and-risk-of-allergic-diseases-a-study-of-two-generations-in-norway
#10
Nils Oskar Jõgi, Cecilie Svanes, Silver Peeter Siiak, Erin Logan, John W Holloway, Jannicke Igland, Ane Johannessen, Michael Levin, Francisco Gomez Real, Vivi Schlunssen, William G C Horsnell, Randi J Bertelsen
BACKGROUND: Animal and human studies indicate that definitive host helminth infections may confer protection from allergies. However, zoonotic helminths, such as Toxocara species (spp.), have been associated with increased allergies. OBJECTIVE: We describe the prevalence of Toxocara spp. and Ascaris spp. seropositivity, and associations with allergic diseases and sensitisation, in two generations in Bergen, Norway. METHODS: Serum levels of total IgG4, anti-Toxocara spp...
November 8, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29116394/a-prospective-randomized-pilot-study-evaluating-an-eras-protocol-versus-a-standard-protocol-for-patients-treated-with-radical-cystectomy-and-urinary-diversion-for-bladder-cancer
#11
Sebastian Karl Frees, Jonathan Aning, Peter Black, Werner Struss, Robert Bell, Claudia Chavez-Munoz, Martin Gleave, Alan I So
PURPOSE: There is a lack of evidence demonstrating the benefits of using enhanced recovery after surgery protocols (ERAS). Here, we propose to use a randomized clinical pilot study to demonstrate the benefits and feasibility of implementing ERAS versus standard protocols (SP) in patients undergoing radical cystectomy (RC) and urinary diversion. METHODS: 27 consecutive patients undergoing RC were included in the study. 12 patients were prospectively randomized to follow an ERAS protocol and 15 patients followed an SP...
November 7, 2017: World Journal of Urology
https://www.readbyqxmd.com/read/29114507/investigation-of-alpha-band-of-the-electroencephalogram-before-and-after-a-task-of-proprioceptive-neuromuscular-facilitation
#12
Luan Correia Costa, Alzira Andrade, Lysnara Lial, Rayele Moreira, Ane Caroline Lima, Astrogildo Magvinier, Ricardo Lira, Anderson Aragão, Pedro Henrique Ulisses, Eric Crespo, Marco Orsini, Silmar Teixeira, Victor Hugo Bastos
The proprioceptive neuromuscular facilitation (PNF) sets up a feature of treatment developed with the objective to facilitate and improve the motor performance. The aim of this study was to investigate in healthy female individuals the effects of electrophysiological of a diagonal of the PNF upper limb. The sample consisted of 30 female participants aged between 18 to 28 years, randomly divided into 3 groups (G1, G2, and G3). The three groups had 2 moments of electroencephalographic signal detection, before and after the task...
August 2017: Journal of Exercise Rehabilitation
https://www.readbyqxmd.com/read/29110481/theoretical-insights-into-unexpected-molecular-core-level-shifts-chemical-and-surface-effects
#13
Ane Sarasola, Mikel Abadía, Celia Rogero, Aran Garcia-Lekue
A set of density-functional theory based tools is employed to elucidate the influence of chem- ical and surface-induced changes on the core level shifts of X-ray photoelectron spectroscopy experiments. The capabilities of our tools are demonstrated by analyzing the origin of an unpredicted component in the N 1s core level spectra of metal phthalocyanine molecules (in particular ZnPc) adsorbed on Cu(110). We address surface induced effects, such as splitting of the lowest unoccupied molecular orbital or local electrostatic effects, demonstrating that these cannot account for the huge core level shift measured experimentally...
November 7, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29110087/outcomes-of-open-versus-laparoscopic-surgery-in-patients-with-rectal-cancer
#14
José M Quintana, Ane Anton-Ladislao, Santiago Lázaro, Nerea Gonzalez, Marisa Bare, Nerea Fernandez de Larrea, Maximino Redondo, Eduardo Briones, Antonio Escobar, Cristina Sarasqueta, Susana Garcia-Gutierrez
PURPOSE: The goal of this study was to compare the effectiveness of laparoscopic with that of open surgery up to 2 years after intervention in patients with rectal cancer. METHODS: This is a prospective cohort study of patients with rectal cancer who underwent surgery (laparoscopic or open) between June 2010 and December 2012 in 22 acute hospitals. Main outcomes were mortality, complications, reoperation, readmission, and patient-reported outcome measures (PROMs), as measured using the EuroQol-5D (EQ-5D), European Organisation for Research and Treatment of Cancer (EORTC) QLQ-Q30 and Q29, the Barthel Index (BI), and the Duke-UNC Functional Social Support Questionnaire at baseline, 1 year, and 2 years after surgery...
November 6, 2017: International Journal of Colorectal Disease
https://www.readbyqxmd.com/read/29109700/exploring-the-genome-and-phenotype-of-multi-drug-resistant-klebsiella-pneumoniae-of-clinical-origin
#15
João Anes, Daniel Hurley, Marta Martins, Séamus Fanning
Klebsiella pneumoniae is an important nosocomial pathogen with an extraordinary resistant phenotype due to a combination of acquired resistant-elements and efflux mechanisms. In this study a detailed molecular characterization of 11 K. pneumoniae isolates of clinical origin was carried out. Eleven clinical isolates were tested for their susceptibilities, by disk diffusion and broth microdilution and interpreted according to CLSI guidelines. Efflux activity was determined by measuring the extrusion of ethidium bromide and biofilm formation was assessed following static growth in Müeller-Hinton and minimal media M9 broths at two temperatures and time points...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29107347/sex-differential-effects-of-diphtheria-tetanus-pertussis-vaccine-for-the-outcome-of-paediatric-admissions-a-hospital-based-observational-study-from-guinea-bissau
#16
Annemette Andersen, Morten Bjerregaard-Andersen, Amabelia Rodrigues, Paulo Umbasse, Ane Bærent Fisker
BACKGROUND: In spite of protection against the targeted infections, a large volume of observational data indicates that diphtheria-tetanus-pertussis (DTP) vaccine may have a negative impact on overall childhood mortality in low-income countries, especially in girls. METHODS: In an observational study using data from Bandim Health Project's continuous registration of all admissions to the paediatric ward at the National Hospital Simão Mendes in Bissau, we investigated whether DTP was associated with higher female than male in-hospital mortality (female/male case fatality ratio (F/M CFR)) and whether the CFR comparing DTP-vaccinated and DTP-unvaccinated children differed by sex...
October 26, 2017: Vaccine
https://www.readbyqxmd.com/read/29102817/low-frequency-fluctuation-of-brain-spontaneous-activity-and-obsessive-compulsive-symptoms-in-a-large-school-age-sample
#17
Marcelo Q Hoexter, Claudinei E Biazoli, Pedro G Alvarenga, Marcelo C Batistuzzo, Giovanni A Salum, Ary Gadelha, Pedro M Pan, Mauricio Anés, Leticia Mancini-Martins, Luciana M Moura, Carles Soriano-Mas, Marco A G Del' Aquilla, Edson Amaro, Luis A Rohde, Andrea P Jackowski, Rodrigo A Bressan, Euripedes C Miguel, Maria C do Rosario, João R Sato
BACKGROUND: The present study was designed to explore alterations in brain dynamics at rest that are associated with Obsessive Compulsive Symptoms (OCS) in childhood by measuring low frequency fluctuation of spontaneous brain activity in a large school community sample from a developing country. METHOD: Resting state functional magnetic resonance imaging data were collected in a sample of 655 children and adolescents (6-15 years old) from the brazilian 'High Risk Cohort Study for Psychiatric Disorders (HRC)'...
October 16, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/29097431/a-simple-algorithm-for-the-identification-of-clinical-copd-phenotypes
#18
Pierre-Régis Burgel, Jean-Louis Paillasseur, Wim Janssens, Jacques Piquet, Gerben Ter Riet, Judith Garcia-Aymerich, Borja Cosio, Per Bakke, Milo A Puhan, Arnulf Langhammer, Inmaculada Alfageme, Pere Almagro, Julio Ancochea, Bartolome R Celli, Ciro Casanova, Juan P de-Torres, Marc Decramer, Andrés Echazarreta, Cristobal Esteban, Rosa Mar Gomez Punter, MeiLan K Han, Ane Johannessen, Bernhard Kaiser, Bernd Lamprecht, Peter Lange, Linda Leivseth, Jose M Marin, Francis Martin, Pablo Martinez-Camblor, Marc Miravitlles, Toru Oga, Ana Sofia Ramírez, Don D Sin, Patricia Sobradillo, Juan J Soler-Cataluña, Alice M Turner, Francisco Javier Verdu Rivera, Joan B Soriano, Nicolas Roche
This study aimed to identify simple rules for allocating chronic obstructive pulmonary disease (COPD) patients to clinical phenotypes identified by cluster analyses.Data from 2409 COPD patients of French/Belgian COPD cohorts were analysed using cluster analysis resulting in the identification of subgroups, for which clinical relevance was determined by comparing 3-year all-cause mortality. Classification and regression trees (CARTs) were used to develop an algorithm for allocating patients to these subgroups...
November 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29097155/the-mechanism-of-action-and-role-of-hydrogen-sulfide-in-the-control-of-vascular-tone
#19
REVIEW
Eleni Dongó, Gabriella Beliczai-Marosi, Ane Stensønes Dybvig, Levente Kiss
Our knowledge about hydrogen sulfide (H2S) significantly changed over the last two decades. Today it is considered as not only a toxic gas but also as a gasotransmitter with diverse roles in different physiological and pathophysiological processes. H2S has pleiotropic effects and its possible mechanisms of action involve (1) a reversible protein sulfhydration which can alter the function of the modified proteins similar to nitrosylation or phosphorylation; (2) direct antioxidant effects and (3) interaction with metalloproteins...
October 30, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29096587/proprioceptive-neuromuscular-facilitation-increases-alpha-absolute-power-in-the-dorsolateral-prefrontal-cortex-and-superior-parietal-cortex
#20
Lysnara Lial, Rayele Moreira, Luan Correia, Alzira Andrade, Ane Caroline Pereira, Ricardo Lira, Rogério Figueiredo, Fernando Silva-Júnior, Marco Orsini, Pedro Ribeiro, Bruna Velasques, Maurício Cagy, Silmar Teixeira, Victor Hugo Bastos
The physiotherapist's clinical practice includes proprioceptive neuromuscular facilitation (PNF), which is a treatment concept that accelerates the response of neuromuscular mechanisms through spiral and diagonal movements. The adaptations that occur in the nervous system following PNF are still poorly described in the literature. Thus, this study had a goal to investigate the electrophysiological changes in the fronto-parietal circuit during PNF and movement in sagittal and diagonal patterns. This study included 30 female participants, who were divided into three groups (control, PNF, and flexion groups)...
September 2017: Somatosensory & Motor Research
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