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https://www.readbyqxmd.com/read/28447539/feasibility-of-preference-driven-radiotherapy-dose-treatment-planning-to-support-shared-decision-making-in-anal-cancer
#1
Heidi S Rønde, Leonard Wee, John Pløen, Ane L Appelt
PURPOSE/OBJECTIVE: Chemo-radiotherapy is an established primary curative treatment for anal cancer, but clinically equal rationale for different target doses exists. If joint preferences (physician and patient) are used to determine acceptable tradeoffs in radiotherapy treatment planning, multiple dose plans must be simultaneously explored. We quantified the degree to which different toxicity priorities might be incorporated into treatment plan selection, to elucidate the feasible decision space for shared decision making in anal cancer radiotherapy...
April 27, 2017: Acta Oncologica
https://www.readbyqxmd.com/read/28445359/the-impact-of-body-contouring-procedures-on-urologic-outcomes-in-massive-weight-loss-patients
#2
Oren P Mushin, Franca S Kraenzlin, Anees Fazili, Ahmed Ghazi, Ronald P Bossert
BACKGROUND: Post-massive weight loss body contouring is a growing area of plastic surgery. Studies have shown preexisting urinary incontinence amelioration in patients undergoing abdominal body-contouring procedures. These studies are small, focus on cosmetic abdominoplasties, and lack use of standardized surveys. The purpose of this study was to evaluate urinary incontinence endpoints in massive weight loss patients undergoing body-contouring procedures. METHODS: A retrospective review was conducted over a 6-year period...
May 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28442327/sample-preservation-transport-and-processing-strategies-for-honeybee-rna-extraction-influence-on-rna-yield-quality-target-quantification-and-data-normalization
#3
Eva Forsgren, Barbara Locke, Emilia Semberg, Ane T Laugen, Joachim R de Miranda
Viral infections in managed honey bees are numerous, and most of them are caused by viruses with an RNA genome. Since RNA degrades rapidly, appropriate sample management and RNA extraction methods are imperative to get high quality RNA for downstream assays. This study evaluated the effect of various sampling-transport scenarios (combinations of temperature, RNA stabilizers, and duration) of transport on six RNA quality parameters; yield, purity, integrity, cDNA synthesis efficiency, target detection and quantification...
April 22, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28416165/influence-of-cardiovascular-risk-in-the-prediction-and-timing-of-cardiac-events-after-exercise-echocardiogram-testing-without-ischemia
#4
Sonia Velasco Del Castillo, Ane Antón Ladislao, Verónica Gómez Sánchez, José Juan Onaindia Gandarias, Ángela Cacicedo Fernández de Bobadilla, Ibon Rodríguez Sánchez, Eva Laraudogoitia Zaldumbide
INTRODUCTION AND OBJECTIVES: There have been no analyses of the influence of cardiovascular risk as a predictor of events in patients with exercise echocardiography (EE) without ischemia. Our objective was to determine the predictors of cardiac events, paying special attention to cardiovascular risk. METHODS: This study included 1640 patients with EE without ischemia. Of these, there were 1206 with no previously known coronary artery disease (CAD), whose risk of a fatal cardiovascular disease event was estimated according to the European SCORE (Systematic COronary Risk Evaluation) risk assessment system, and 434 with known CAD...
April 13, 2017: Revista Española de Cardiología
https://www.readbyqxmd.com/read/28415335/effectively-explore-metastable-states-of-proteins-by-adaptive-nonequilibrium-driving-simulations
#5
Biao Wan, Shun Xu, Xin Zhou
Nonequilibrium drivings applied in molecular dynamics (MD) simulations can efficiently extend the visiting range of protein conformations, but might compel systems to go far away from equilibrium and thus mainly explore irrelevant conformations. Here we propose a general method, called adaptive nonequilibrium simulation (ANES), to automatically adjust the external driving on the fly, based on the feedback of the short-time average response of system. Thus, the ANES approximately keeps the local equilibrium but efficiently accelerates the global motion...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28412340/levan-promotes-antiproliferative-and-pro-apoptotic-effects-in-mcf-7-breast-cancer-cells-mediated-by-oxidative-stress
#6
Eveline A I F Queiroz, Zuleica B Fortes, Mário A A da Cunha, Hande Kazak Sarilmiser, Aneli M Barbosa Dekker, Ebru Toksoy Öner, Robert F H Dekker, Neelam Khaper
Exopolysaccharides are high-valued bio-products produced by various microbial species and have been described to possess biological response modifying activities. These bio-products have been effective as therapeutic agents in various human disease conditions. The objective of this study was to examine the effects of levan (a (2→6)-β-d-fructan) produced on sucrose by the halophilic bacterium, Halomonas smyrnensis AAD6(T), in human breast cancer MCF-7 cells. MCF-7 cells were exposed to levan for 24 and 48h...
April 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28411942/transplacental-nutrient-transfer-in-the-human-in%C3%A2-vivo-determined-by-4-vessel-sampling
#7
Trond M Michelsen, Ane M Holme, Tore Henriksen
BACKGROUND: The intrauterine environment and especially the fetal nutritional conditions affect lifelong health. There are few human in vivo studies on fetal nutrition. The importance to test experimentally based concepts of fetal nutrition in a human in vivo setting is becoming increasingly apparent. A way of testing nutrient transfer in human is 4-vessel sampling, which implies blood sampling from artery and vein on both sides of the placenta. Here we give a brief review of the studies using the 4-vessel sampling method...
March 24, 2017: Placenta
https://www.readbyqxmd.com/read/28409852/tandem-mass-spectrometric-analysis-of-novel-peptide-modified-gemini-surfactants-used-as-gene-delivery-vectors
#8
Mays Al-Dulaymi, Anas El-Aneed
Diquaternary ammonium gemini Surfactants have emerged as effective gene delivery vectors. A novel series of eleven peptide-modified compounds was synthesized, showing promising results in delivering genetic materials. The purpose of this work is to elucidate the tandem mass spectrometric (MS/MS) dissociation behaviour of these novel molecules establishing a generalized MS/MS fingerprint. Exact mass measurements were achieved using a hybrid quadrupole orthogonal time-of-flight mass spectrometer (QqToF-MS) and a multi-stage tandem mass spectrometric analysis was conducted using a triple quadrupole-linear ion trap mass spectrometer (QqQLIT-MS)...
April 14, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28409296/silver-i-complexes-with-chromone-derived-hydrazones-investigation-on-the-antimicrobial-and-cytotoxic-effects
#9
Lenka V Tamayo, Ane F Santos, Isabella P Ferreira, Verlane G Santos, Miriam T P Lopes, Heloisa Beraldo
Complexes [Ag(HCrPh)2]NO3·2H2O (1) and [Ag(HCrpClPh)2]NO3 (2) were obtained with 3-formyl-6-methylchromone-phenyl hydrazone (HCrPh, HL1) and 3-formyl-6-methylchromone-para-chloro-phenyl hydrazone (HCrpClPh, HL2). Although the hydrazones were inactive, upon coordination to silver(I) antifungal activity significantly improved against several Candida strains. Complexes (1-2) revealed to be more active than silver nitrate, silver sulfadiazine and the reference drug nystatin against Candida parapsilosis. The cytotoxic activities of the hydrazones and their silver(I) complexes were evaluated in comparison with cisplatin on B16F10 (metastatic melanoma) and Melan-a (non-tumorigenic melanocyte) cells...
April 13, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28401870/response-of-narrow-qrs-tachycardia-to-premature-atrial-extra-what-is-the-mechanism
#10
Krishna Kumar Mohanan Nair, Narayanan Namboodiri, Bharatraj Banavalikar, Sreevilasam Pushpangadhan Abhilash, Anees Thajudeen, Ajitkumar Valaparambil
A 60-year-old woman was referred for radiofrequency catheter ablation of narrow QRS tachycardia that was terminated with intravenous adenosine. Twelve-lead ECG showed no baseline preexcitation. Echocardiogram was essentially normal. The electrophysiological study showed a normal AH interval of 114 ms and HV interval of 48 ms during sinus rhythm. Anterograde study demonstrated no dual AV nodal physiology. Atrial pacing protocols easily and reproducibly induced narrow QRS tachycardia (Fig. 1A & 1B). Premature atrial extra (PAE) stimuli were delivered during the tachycardia (Fig...
November 2016: Indian Pacing and Electrophysiology Journal
https://www.readbyqxmd.com/read/28401858/wide-qrs-tachycardia-what-is-the-mechanism
#11
Krishna Kumar Mohanan Nair, Narayanan Namboodiri, Sreevilasam Pushpangadhan Abhilash, Anees Thajudeen, Ajitkumar Valaparambil
No abstract text is available yet for this article.
January 2017: Indian Pacing and Electrophysiology Journal
https://www.readbyqxmd.com/read/28394556/%C3%AE-cd-functionalized-microdevice-for-rapid-capture-and-release-of-bacteria
#12
Alexandra Perez-Anes, Anna Szarpak-Jankowska, Dorothée Jary, Rachel Auzély-Velty
Most procedures for detecting pathogens in liquid media require an initial concentration step. In this regard, carbohydrates have proven to be attractive affinity ligands for the solid-phase capture of bacteria that use lectins for adhesion to host cell membranes. However, the use of cyclodextrin-immobilized substrates to selectively trap bacteria has not been explored before. Here, using quartz-crystal microbalance with dissipation monitoring experiments, we demonstrate that functionalization of surfaces by β-cyclodextrin (β-CD) can not only allow for rapid and efficient capture of bacterial cells in liquid but also their facile elution with an aqueous solution of a selectively methylated β-CD derivative as a competitive molecule...
April 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28391435/evaluation-of-different-treatment-protocols-for-dentin-hypersensitivity-an-18-month-randomized-clinical-trial
#13
Anely Oliveira Lopes, Carlos de Paula Eduardo, Ana Cecília Correa Aranha
This randomized and longitudinal in vivo study aimed to assess different protocols for the treatment of dentin hypersensitivity with low-power laser (with different doses), high-power laser, and a desensitizing agent, for a period of 12 and 18 months. The lesions from 32 patients (117 lesions), who were submitted to the inclusion and exclusion criteria, were divided into nine groups (n = 13): G1: Gluma Desensitizer (Heraeus Kulzer), G2: low-power laser with low dose (three points of irradiation in vestibular portion and an apical point 30 mW, 10 J/cm(2), 9 s per point with the wavelength of 810 nm, with three sessions with an interval of 72 h), G3: low-power laser with high dose (one point in the cervical area, and one apical point 100 mW, 40 J/cm(2), 11 s per point with the wavelength of 810 nm in three sessions with an interval of 72 h), G4: low-power laser with low dose + Gluma Desensitizer, G5: low-power laser with high dose + Gluma Desensitizer, G6: Nd:YAG laser (Power Laser™ ST6, Research® in contact 1...
April 8, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28387418/delineating-the-role-of-ripples-on-the-thermal-expansion-of-2d-honeycomb-materials-graphene-2d-h-bn-and-monolayer-ml-mos2
#14
P Anees, M C Valsakumar, B K Panigrahi
We delineated the role of thermally excited ripples on the thermal expansion properties of 2D honeycomb materials (free-standing graphene, 2D h-BN, and ML-MoS2), by explicitly carrying out three-dimensional (3D) and two-dimensional (2D) molecular dynamics simulations. In 3D simulations, the in-plane lattice parameter (a-lattice) of graphene and 2D h-BN shows thermal contraction over a wide range of temperatures and exhibits a strong system size dependence. The 2D simulations of the very same system show a reverse trend, where the a-lattice expands in the whole computed temperature range...
April 19, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28385217/undec-10-enoic-acid-functionalized-chitosan-based-novel-nano-conjugate-an-enhanced-anti-bacterial-biofilm-and-anti-cancer-potential
#15
Khairujjaman Laskar, Syed Mohd Faisal, Abdul Rauf, Anees Ahmed, Mohammad Owais
Fatty acid functionalized chitosan conjugates are of great interest in cancer therapeutics because of its internalization through receptor mediated endocytosis into the cancer cells. Keeping the above fact into consideration, herein we synthesized the undec-10-enoic acid functionalized chitosan based undecyl-chitosan (U-CS) nano-bioconjugate with the use of DCC as a coupling agent. The U-CS conjugate synthesized was confirmed and characterized by FTIR, (1)H NMR, TGA, XRD, SEM and TEM analysis. Generally, it is well established that conjugates of oleic acid with human Alpha-lactalbumin (HAMLET) induce cytotoxicity in the altered cells, but not in healthy cells...
June 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28382337/physiochemical-properties-of-greatly-enhanced-photoluminescence-of-aqueous-dispersible-upconversion-caf2-yb-er-nanoparticles
#16
Anees A Ansari, Ranvijay Yadav, S B Rai
Crystal phase morphological structure and optical properties of the as-prepared upconversion CaF2:Yb/Er(core) and sequential coating of an inert crystalline material and silica layers surrounding the seed core-nanoparticles (NPs) were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED), optical absorption, FTIR spectroscopy and upconversion photoluminescence spectroscopy. Owing to the unique properties of CaF2 host matrix, we realized their practical applications in biomedical science to improve the upconversion luminescence property and aqueous dispersibility...
April 6, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28380129/factors-associated-with-user-satisfaction-regarding-treatment-offered-in-brazilian-primary-health-care
#17
Ane Polline Lacerda Protasio, Luciano Bezerra Gomes, Liliane Dos Santos Machado, Ana Maria Gondim Valença
The Program for Access and Quality Improvement in Primary Care (PMAQ-AB) aimed to improve healthcare public service quality. The purpose of this study was to identify the main factors that influence user satisfaction in Brazilian primary health care services. This research was carried out using secondary data from the first cycle of the PMAQ-AB. A cluster analysis was carried out to find the dependent variable of user satisfaction; and logistic regression was applied in order to obtain the decision model. From the resulting regression model, two factors can be highlighted as regards influencing user satisfaction in Brazil: the user's perception that the team did not attempt to address their needs/problems within the health unit; and the user not feeling respected by the professionals in relation to cultural habits, customs, and religion or only sometimes feeling so...
March 30, 2017: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/28378935/the-pathogenic-development-of-sclerotinia-sclerotiorum-in-soybean-requires-specific-host-nadph-oxidases
#18
Ashish Ranjan, Dhileepkumar Jayaraman, Craig Grau, John H Hill, Steven A Whitham, Jean-Michel Ané, Damon L Smith, Mehdi Kabbage
The plant membrane localized NADPH oxidases, also known as respiratory burst oxidase homologs (RBOHs), play crucial roles in various cellular activities including plant disease responses, and are a major source of reactive oxygen species (ROS). Sclerotinia sclerotiorum is a cosmopolitan fungal pathogen that causes Sclerotinia stem rot (SSR) in soybean. Via a key virulence factor, oxalic acid, it induces programmed cell death (PCD) in the host plant, a process that is reliant on ROS generation. In this study, using protein sequence similarity searches, we have identified seventeen soybean RBOHs (GmRBOHs) and studied their contribution to SSR disease development, drought tolerance and nodulation...
April 5, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28378527/the-prognostic-value-of-simultaneous-tumor-and-serum-ras-raf-mutations-in-localized-colon-cancer
#19
Caroline Emilie B Thomsen, Ane Lindegaard Appelt, Rikke Fredslund Andersen, Jan Lindebjerg, Lars Henrik Jensen, Anders Jakobsen
The impact of RAS/RAF mutations in localized colon cancer needs clarification. Based on analysis of tumor-specific DNA, this study aimed at elucidating the prognostic influence of mutational status in tumor and serum using an extended panel of mutations. The study retrospectively included 294 patients with curatively resected stage I-III adenocarcinoma of the colon. Mutations in tumor and serum were determined at time of surgery. Analyses were performed with droplet digital PCR technology. Hazard ratio (HR) for the association between mutational status and survival was estimated in multivariate analysis taking known prognostic factors into account...
April 4, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28375931/a-comparison-of-the-detection-of-sentinel-lymph-nodes-using-indocyanine-green-and-near-infrared-fluorescence-imaging-versus-blue-dye-during-robotic-surgery-in-uterine-cancer
#20
Ane Gerda Eriksson, Anna Beavis, Robert A Soslow, Qin Zhou, Nadeem R Abu-Rustum, Ginger J Gardner, Oliver Zivanovic, Kara Long Roche, Yukio Sonoda, Mario M Leitao, Elizabeth L Jewell
OBJECTIVES: The objective of this study was to assess and compare the sentinel lymph node (SLN) detection rate with indocyanine green (ICG) and near-infrared fluorescence imaging versus blue dye using the robotic platform in patients with uterine cancer. METHODS: We identified all patients with uterine cancer undergoing SLN mapping using ICG or blue dye on the robotic platform from January 2011 to December 2013. Our institutional SLN algorithm and pathologic processing protocol were adhered to uniformly...
May 2017: International Journal of Gynecological Cancer
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