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https://www.readbyqxmd.com/read/28806688/concentration-dependent-transcriptome-responses-of-zebrafish-embryos-after-exposure-to-cadmium-cobalt-and-copper
#1
Laura Sonnack, Thorsten Klawonn, Ralf Kriehuber, Henner Hollert, Christoph Schäfers, Martina Fenske
Environmental metals are known to cause harmful effects to fish of which many molecular mechanisms still require elucidation. Particularly concentration dependence of gene expression effects is unclear. Focusing on this matter, zebrafish embryo toxicity tests were used in combination with transcriptomics. Embryos were exposed to three concentrations of copper (CuSO4), cadmium (CdCl2) and cobalt (CoSO4) from just after fertilization until the end of the 48hpf pre- and 96hpf post-hatch stage. The RNA was then analyzed on Agilent's Zebrafish (V3, 4×44K) arrays...
August 2, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28806626/cryopreservation-of-cynomolgus-macaque-macaca-fascicularis-sperm-with-glycerol-and-ethylene-glycol-and-its-effect-on-sperm-specific-ion-channels-catsper-and-hv1
#2
Bingbing Chen, Shanshan Li, Yaping Yan, Yanchao Duan, Shaohui Chang, Hong Wang, Weizhi Ji, Xin Wu, Wei Si
The cryoprotective agent (CPA) is one of the most important factors that affects the cryosurvival of sperm. The aim of the present study was to compare two different CPAs, glycerol (Gly) and ethylene glycol (EG), on the cryopreservation of cynomolgus macaques sperm and evaluate the effects of cryopreservation on sperm motility, acrosomal integrity, DNA integrity, mitochondrial function and the sperm membrane ion channels CatSper and Hv1. Compared to fresh sperm, cryopreservation with either 0.7 M Gly or EG decreased the sperm motility (79...
August 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/28806625/association-of-changes-among-body-condition-score-during-the-transition-period-with-nefa-and-bhba-concentrations-milk-production-fertility-and-health-of-holstein-cows
#3
R V Barletta, M Maturana Filho, P D Carvalho, T A Del Valle, A S Netto, F P Rennó, R D Mingoti, J R Gandra, G B Mourão, P M Fricke, R Sartori, E H Madureira, M C Wiltbank
Our objective was to evaluate the association between body condition score (BCS) change during the transition period with fertility, non-esterified fatty acids (NEFA) and beta-hydroxybutyrate (BHBA) concentrations, milk yield, and health problems of Holstein cows in a retrospective cohort study. Holstein cows (n = 232) were assessed for BCS (5 point scale; 0.25 point increments) and had blood collected at 21 and 7 d before, on the day of, and 7 and 21 d after calving. Blood samples were assayed for NEFA and BHBA concentrations...
July 27, 2017: Theriogenology
https://www.readbyqxmd.com/read/28806553/thiacloprid-adsorption-and-leaching-in-soil-effect-of-the-composition-of-irrigation-solutions
#4
José Antonio Rodríguez-Liébana, M Dolores Mingorance, Aránzazu Peña
Pressure on groundwater resources has increased during the last decades due to the growing demand, especially in arid and semiarid regions, such as the Mediterranean basin, with frequent drought periods. In order to partially remediate this environmental problem of world concern, irrigation of agricultural lands with adequately treated wastewaters (TWW) is becoming a common management practice. The complex composition of these low-quality waters may influence the behavior of organic contaminants in soils. A calcareous soil with low organic carbon content was selected for the assessment of the adsorption and leaching of the neonicotinoid insecticide thiacloprid (THC)...
August 11, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28806549/liming-impacts-on-soils-crops-and-biodiversity-in-the-uk-a-review
#5
REVIEW
J E Holland, A E Bennett, A C Newton, P J White, B M McKenzie, T S George, R J Pakeman, J S Bailey, D A Fornara, R C Hayes
Fertile soil is fundamental to our ability to achieve food security, but problems with soil degradation (such as acidification) are exacerbated by poor management. Consequently, there is a need to better understand management approaches that deliver multiple ecosystem services from agricultural land. There is global interest in sustainable soil management including the re-evaluation of existing management practices. Liming is a long established practice to ameliorate acidic soils and many liming-induced changes are well understood...
August 11, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28806546/parental-exposure-to-bisphenol-a-and-its-analogs-influences-zebrafish-offspring-immunity
#6
Xing Dong, Zhen Zhang, Shunlong Meng, Chenyuan Pan, Ming Yang, Xiangyang Wu, Liuqing Yang, Hai Xu
Transgenerational effects of environmental pollutants on humans and animals are complex. Thus, we used zebrafish to evaluate the effects of parental whole-life cycle exposure to bisphenol A and its analogs (bisphenol S and F) on offspring innate immunity. At adulthood, offspring were examined with/without continued chemicals treatment until 72h post-fertilization (hpf). To measure offspring immune function, larvae at 72 hpf were expose for 24h with/without the viral mimic polyinosinic-cytidylic acid (Poly I:C) or the bacterial mimic Pam3Cys-Ser-Lys4 (PAM3CSK4)...
August 11, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28806103/hsp83-loss-suppresses-proteasomal-activity-resulting-in-an-upregulation-of-caspase-dependent-compensatory-autophagy
#7
Courtney Choutka, Lindsay DeVorkin, Nancy Erro Go, Ying-Chen Claire Hou, Annie Moradian, Gregg B Morin, Sharon M Gorski
The 2 main degradative pathways that contribute to proteostasis are the ubiquitin-proteasome system and autophagy but how they are molecularly coordinated is not well understood. Here, we demonstrate an essential role for an effector caspase in the activation of compensatory autophagy when proteasomal activity is compromised. Functional loss of Hsp83, the Drosophila ortholog of human HSP90 (heat shock protein 90), resulted in reduced proteasomal activity and elevated levels of the effector caspase Dcp-1. Surprisingly, genetic analyses showed that the caspase was not required for cell death in this context, but instead was essential for the ensuing compensatory autophagy, female fertility, and organism viability...
August 14, 2017: Autophagy
https://www.readbyqxmd.com/read/28805926/multiple-trait-genomewide-mapping-and-gene-network-analysis-for-scrotal-circumference-growth-curves-in-brahman-cattle
#8
A C C Soares, S E F Guimarães, M J Kelly, M R S Fortes, F F E Silva, L L Verardo, R Mota, S Moore
Fertility traits are economically important in cattle breeding programs. Scrotal circumference (SC) measures are repeatable, easily obtained, highly heritable, and positively correlated with female fertility traits and sperm quality traits in males. A useful approach to summarize SC measures over time is using nonlinear models, which summarize specific measures of SC in a few parameters with biological interpretation. This approach facilitates the selection of bulls with larger SC and maturity index (K), that is, early maturing animals...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805643/the-evolution-of-cuticular-fertility-signals-in-eusocial-insects
#9
REVIEW
Adrian A Smith, Jürgen Liebig
A reproductive division of labor is a definitive characteristic of eusocial insect societies and it requires a means through which colony members can assess the presence and productivity of reproductive individuals. Cuticular hydrocarbons are the primary means of doing so across eusocial hymenopterans. However, recent experimental work presents conflicting views on how these chemical signals function, are interpreted by workers, and evolve. These recent advances include demonstrations of hydrocarbons as evolutionarily conserved 'queen pheromones' and as species-divergent 'fertility signals' used by both queens and workers...
August 2017: Current Opinion in Insect Science
https://www.readbyqxmd.com/read/28805606/detection-of-sea-type-%C3%AE-thalassemia-in-embryo-biopsies-by-digital-pcr
#10
Ta-Hsien Lee, Ya-Chiung Hsu, Chia Lin Chang
OBJECTIVE: Accurate and efficient pre-implantation genetic diagnosis (PGD) based on the analysis of single or oligo-cells is needed for timely identification of embryos that are affected by deleterious genetic traits in in vitro fertilization (IVF) clinics. Polymerase chain reaction (PCR) is the backbone of modern genetic diagnoses, and a spectrum of PCR-based techniques have been used to detect various thalassemia mutations in prenatal diagnosis (PND) and PGD. Among thalassemias, SEA-type α-thalassemia is the most common variety found in Asia, and can lead to Bart's hydrops fetalis and serious maternal complications...
August 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28805599/nationwide-trends-and-in-hospital-complications-of-trachelectomy-for-surgically-resectable-cervical-cancer-in-taiwanese-women-a%C3%A2-population-based-study-1998-2013
#11
Jerry Cheng-Yen Lai, Hung-Hui Chen, Kuei-Hui Chu, Chia-Sui Weng, Yiing-Jenq Chou, Nicole Huang, Yu-Ju Chien, Kung-Liahng Wang
OBJECTIVE: To describe the nationwide trends in the utilization of trachelectomy among Taiwanese women with surgically resectable cervical cancer over the past decade, and to compare associated demographic characteristics, in-hospital complications and related outcomes using a population-based dataset from 1998 to 2013. MATERIALS AND METHODS: We conducted a population-based, cohort study using inpatient admission claims data of Taiwan's National Health Insurance program...
August 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28805279/extreme-weather-year-sequences-have-non-additive-effects-on-environmental-nitrogen-losses
#12
Javed Iqbal, Magdalena Necpalova, Sotirios V Archontoulis, Robert P Anex, Marie Bourguignon, Daryl Herzmann, David C Mitchell, John E Sawyer, Qing Zhu, Michael J Castellano
The frequency and intensity of extreme weather years, characterized by abnormal precipitation and temperature, are increasing. In isolation, these years have disproportionately large effects on environmental N losses. However, the sequence of extreme weather years (e.g., wet-dry vs. dry-wet) may affect cumulative N losses. We calibrated and validated the DAYCENT ecosystem process model with a comprehensive set of biogeophysical measurements from a corn-soybean rotation managed at three N fertilizer inputs with and without a winter cover crop in Iowa, USA...
August 14, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28805257/three-representative-inter-and-intra-subspecific-crosses-reveal-the-genetic-architecture-of-reproductive-isolation-in-rice
#13
Guangwei Li, Xiaoting Li, Yuan Wang, Jiaming Mi, Feng Xing, Dahan Zhang, Qiyan Dong, Xianghua Li, Jinghua Xiao, Qifa Zhang, Yidan Ouyang
Systematic characterization of genetic and molecular mechanisms in formation of hybrid sterility is of fundamental importance in understanding reproductive isolation and speciation. Using ultra-high-density genetic maps, 43 single-locus QTLs and 223 digenic interactions for embryo-sac, pollen, and spikelet fertility are depicted from three crosses between representative varieties of japonica and two varietal groups of indica, which provide an extensive archive for investigating the genetic basis of reproductive isolation in rice...
August 14, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28805023/onion-allium-cepa-l-peel-extract-ope-regulates-human-sperm-motility-via-protein-kinase-c-mediated-activation-of-the-human-voltage-gated-proton-channel
#14
M R Chae, S J Kang, K P Lee, B R Choi, H K Kim, J K Park, C Y Kim, S W Lee
Onion (Allium cepa L.) and quercetin protect against oxidative damage and have positive effects on multiple functional parameters of spermatozoa, including viability and motility. However, the associated underlying mechanisms of action have not yet been identified. The aim of this study was to investigate the effect of onion peel extract (OPE) on voltage-gated proton (Hv1) channels, which play a critical role in rapid proton extrusion. This process underlies a wide range of physiological processes, particularly male fertility...
August 13, 2017: Andrology
https://www.readbyqxmd.com/read/28804986/effects-of-hydroxycitric-acid-on-lipid-droplet-accumulation-in-chicken-embryos
#15
Mengling Peng, Longlong Li, Lei Yu, Chongyang Ge, Haitian Ma
This study was conducted to determine the impact of (-)-hydroxycitric acid ((-)-HCA) on biochemical indices and lipid metabolism parameters in chicken embryos. Two hundred and forty fertilized eggs were divided into six groups and injected with (-)-HCA at concentrations of 0, 0.1, 0.5, 1.0, 10.0 and 50 mg/kg (n = 40). After 19 days of incubation, serum and liver were collected for analysis of biochemical indices and lipid metabolism parameters. Results showed no significant differences on serum biochemical indices: 1-50 mg/kg (-)-HCA significantly increased serum glucose and hepatic glycogen contents (P < 0...
August 14, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28804928/the-comet-assay-for-detection-of-dna-damage-in-canine-sperm
#16
A F Pereira, P Borges, A Fontbonne, L Cardoso, I Gaivão, A Martins-Bessa
Sperm DNA integrity is a fundamental prerequisite in fertilization and embryo development. Among DNA integrity tests, the Comet assay is an accurate and sensitive test for the detection of sperm oxidative damage. The aim of this work was to evaluate sperm oxidative damage using the Comet assay and to study the correlation between Comet and routine assays for the evaluation of semen quality. Dogs were divided in two groups: group A (n = 6), comprising dogs with abnormal spermiogram, that is astheno-, terato- or oligoasthenoteratozoospermic (OAT); and group B (n = 8), comprising normospermic dogs...
August 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28804859/fertility-life-table-of-tetranychus-palmarum-flechtmann-noronha-acari-tetranychidae-in-oil-palm
#17
A C S Noronha, C T Ferreira, E J M Tavares, D B Lima
Some species of spider mites belonging to the Tetranychidae family are known to associate with oil palm (Elaeis guineensis Jacq. - Arecaceae). The occurrence of Tetranychus palmarum Flechtmann & Noronha (Acari, Tetranychidae) was verified on oil palm seedlings under greenhouse conditions in the State of Pará in Northern Brazil. Plants with colonies of T. palmarum presented yellowish spots on leaflets and leaves with chlorosis. The objective of this study was to access the biology and fertility life table of T...
August 13, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28804762/-super-bowl-babies-do-counties-with-super-bowl-winning-teams-experience-increases-in-births-nine-months-later
#18
George M Hayward, Anna Rybińska
Following the claim of a highly publicized National Football League (NFL) commercial, we test whether the Super Bowl provides a positive exogenous shock to fertility in counties of winning teams. Using stadium locations to identify teams' counties, we analyze the number of births in counties of both winning and losing teams for ten recent Super Bowls. We also test for state effects and general effects of the NFL playoffs. Overall, our results show no clear pattern of increases in the number of births in winning counties nine months after the Super Bowl...
January 2017: Socius
https://www.readbyqxmd.com/read/28803846/embryo-survival-and-birth-after-minimum-volume-vitrification-or-slow-freezing-of-in-vivo-and-in-vitro-produced-ovine-embryos
#19
P C Dos Santos-Neto, F Cuadro, N Barrera, M Crispo, A Menchaca
The objective was to evaluate pregnancy outcomes and birth rate of in vivo derived vs. in vitro produced ovine embryos submitted to different cryopreservation methods. A total of 197 in vivo and 240 in vitro produced embryos were cryopreserved either by conventional freezing, or by vitrification with Cryotop or Spatula MVD methods on Day 6 after insemination/fertilization. After thawing/warming and transfer, embryo survival rate on Day 30 of gestation was affected by the source of the embryos (in vivo 53.3%, in vitro 20...
August 10, 2017: Cryobiology
https://www.readbyqxmd.com/read/28803842/experimental-evidence-for-hybridization-of-closely-related-lineages-in-plasmodium-relictum
#20
Vaidas Palinauskas, Rasa Bernotienė, Rita Žiegytė, Staffan Bensch, Gediminas Valkiūnas
Over 50 avian Plasmodium species have been described. However, PCR-based information shows much broader diversity of genetic lineages in these parasites. This discrepancy indicates insufficient knowledge about taxonomic diversity and boundaries of a single species in avian Plasmodium species. In recent taxonomy, most of genetically closely related lineages that share the same morphology and development patterns are attributed to the same biological species, but there is no information if these lineages are able to cross...
August 10, 2017: Molecular and Biochemical Parasitology
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