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https://www.readbyqxmd.com/read/28938620/pathological-expression-of-tissue-factor-confers-promising-antitumor-response-to-a-novel-therapeutic-antibody-sc1-in-triple-negative-breast-cancer-and-pancreatic-adenocarcinoma
#1
Xuesai Zhang, Qingrou Li, Hui Zhao, Lanping Ma, Tao Meng, Jianchang Qian, Rui Jin, Jingkang Shen, Ker Yu
The pathological presence of tissue factor (TF) in cancer cells promotes tumor-initiated thrombosis and cancer metastasis. We found that TF is aberrantly present in large percentage of aggressive triple negative breast cancer (TNBC) and pancreatic adenocarcinoma (PaC), two most lethal forms of malignancy that urgently need effective treatment. TF expression in TNBC clustered with higher levels of vimentin, basal-type keratins KRT5/14 and caveolin-1 but lower levels of luminal-type biomarkers. We developed a novel and specific anti-TF therapeutic antibody SC1, which displayed an exceedingly high potency against TF extracellular domain (EC50: 0...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938611/dickkopf-1-induces-angiogenesis-via-vegf-receptor-2-regulation-independent-of-the-wnt-signaling-pathway
#2
Sung Hoon Choi, Hyemi Kim, Hyun Gyu Lee, Beom Kyung Kim, Jun Yong Park, Do Young Kim, Sang Hoon Ahn, Kwang-Hyub Han, Seung Up Kim
Tumor angiogenesis is essential for invasive tumor growth and metastasis. Dickkopf-1 (DKK-1), an antagonist of Wnt signaling, participates in tumor development and progression. We evaluated whether DKK-1 stimulation induces angiogenesis and the endothelial-mesenchymal transition (EnMT). Human umbilical vein endothelial cells (HUVECs) were stimulated with recombinant DKK-1 (rDDK-1) or conditioned medium from a culture of DKK-1-transfected 293 cells. Following stimulation, the expression levels of angiogenesis-related factors and EnMT related markers were determined by immunoblot assays...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938541/differential-expression-of-circulating-biomarkers-of-tumor-phenotype-and-outcomes-in-previously-treated-non-small-cell-lung-cancer-patients-receiving-erlotinib-vs-cytotoxic-chemotherapy
#3
Mary Jo Fidler, Casey Frankenberger, Richard Seto, Gabriela C Lobato, Cristina L Fhied, Selina Sayidine, Sanjib Basu, Mark Pool, Reem Karmali, Marta Batus, Wen-Rong Lie, David Hayes, Jehangir Mistry, Philip Bonomi, Jeffrey A Borgia
BACKGROUND: The objective of this study was to identify serum biomarkers capable of predicting clinical outcomes in previously-treated NSCLC patients with wild-type for EGFR activating mutations or insufficient tissue for mutation status determination. METHODS: Sixty-six Luminex immunoassays representative of biological themes that emerged from a re-analysis of transcriptome data from the Cancer Genome Atlas (TCGA) were evaluate against pretreatment serum specimens from previously-treated advanced NSCLC patients received either cytotoxic chemotherapy (n=32) or erlotinib (n=79)...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938159/lunatic-fringe-and-p53-cooperatively-suppress-mesenchymal-stem-like-breast-cancer
#4
Wen-Cheng Chung, Shubing Zhang, Lavanya Challagundla, Yunyun Zhou, Keli Xu
Claudin-low breast cancer (CLBC) is a poor prognosis molecular subtype showing stemness and mesenchymal features. We previously discovered that deletion of a Notch signaling modulator, Lunatic Fringe (Lfng), in the mouse mammary gland induced a subset of tumors resembling CLBC. Here we report that deletion of one copy of p53 on this background not only accelerated mammary tumor development but also led to a complete penetrance of the mesenchymal stem-like phenotype. All mammary tumors examined in the Lfng/p53 compound mutant mice displayed a mesenchymal/spindloid pathology...
September 19, 2017: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/28937375/superficial-cd34-positive-fibroblastic-tumor-a-new-entity-case-report-and-review-of-literature
#5
Neelam Sood, Binit Kumar Khandelia
Earlier, categorized under low grade malignant fibrous histiocytoma and low grade sarcomas, 'superficial CD34 positive fibroblastic tumor', a newly proposed entity, is a low grade mesenchymal tumor of intermediate malignant potential. Morphological features include monomorphic neoplastic spindle cells arranged in a fascicular to storiform pattern along with sheets of pleomorphic epithelioid cells, and multinucleated giant cells with glassy cytoplasm. Diffuse vimentin and characteristic diffuse CD34 positivity is seen in all cases with few showing focal cytokeratin expression...
July 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28937365/immunohistochemical-characterization-of-glandular-elements-in-glandular-cardiac-myxoma-study-of-six-cases
#6
Devajit Nath, Sudheer Arava, Ruma Ray, Amol Kumar Bhoje, Rachit Saxena, Shiv Kumar Chaudhary
Back ground: Glandular cardiac myxoma has varying clinical presentation with uncertain histogenesis and debatable immunohistochemical profile. Glandular epithelial differentiations are rare phenomenon known to be present as an intrinsic component of the tumor. The origin of the glands has been attributed to epithelial differentiation of a totipotent cardiomyogenic precursor cells or the entrapped foregut rests in the tumor. MATERIALS AND METHODS: Retrospective study includes six cases of glandular cardiac myxoma collected over a perior of 4 years...
July 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28937041/evaluation-of-circulating-endometrial-cells-as-a-biomarker-for-endometriosis
#7
Ying Chen, Hong-Lan Zhu, Zhe-Wen Tang, Kuang Hong Neoh, Dong-Fang Ouyang, Heng Cui, Hong-Yan Cheng, Rui-Qiong Ma, Xue Ye, Ray Ps Han, Xiao-Hong Chang
BACKGROUND: Circulating endometrial cells (CECs) have been reported to be present in the peripheral blood of women with endometriosis (EM), providing clear and specific evidence of the presence of ectopic lesions. In this study, we established a method with a high detection rate of CECs, assessed the diagnostic value of CECs for EM and compared with serum CA125, and proposed a hypothesis for the pathogenesis of EM from the new perspective of CECs. METHODS: The participants were enrolled prospectively from October 2015 to July 2016...
October 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28935373/human-alpha-a-crystallin-missing-n-terminal-domain-poorly-complexes-with-filensin-and-phakinin
#8
Jose M Chaves, Ratna Gupta, Kiran Srivastava, Om Srivastava
The aim of this study was to determine relative importance of N-terminal domain and C-terminal extension of αA-crystallin during their in vitro complex formation with phakinin and filensin (the two lens-specific intermediate filament [IF] proteins). Cloned phakinin, filensin and vimentin were purified under a denaturing conditions by consecutive DEAE-cellulose-, hydroxyapatite- and Sephadex G-75-column chromatographic methods. WTαA-crystallin, αA-NT (N-terminal domain [residue number 1-63])-deleted and αA-CT (C-terminal terminal extension [residue number 140-173]-deleted), were cloned in pET 100 TOPO vector, expressed in BL-21 (DE3) cells using 1% IPTG, and purified using a Ni(2+)-affinity column...
September 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28934754/cntn-1-enhances-chemoresistance-in-human-lung-adenocarcinoma-through-induction-of-epithelial-mesenchymal-transition-by-targeting-the-pi3k-akt-pathway
#9
Ruijie Zhang, Shenghua Sun, Fuyun Ji, Chun Liu, Hua Lin, Lihua Xie, Honghui Yang, Wenxiang Tang, Yan Zhou, Jianping Xu, Pei Li
BACKGROUND/AIMS: Chemoresistance has been a major obstacle to the effective treatment of lung cancer. Previously, we found that contactin-1 (CNTN-1) is related to cisplatin resistance in lung adenocarcinoma. Here, we aimed to investigate the underlying mechanism behind the role of CNTN-1 in cisplatin resistance in lung adenocarcinoma. METHODS: EMT-associated phenotypes, including alterations in cellular morphology and marker (E-cadherin, N-cadherin and Vimentin) expression, were compared between A549 cells and A549/DDP cells (a cisplatin-resistant cell line of lung adenocarcinoma with abnormal CNTN-1 expression) by using real-time time PCR and Western blotting...
September 1, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28934230/e-cadherin-a-determinant-molecule-associated-with-ovarian-cancer-progression-dissemination-and-aggressiveness
#10
Marina Rosso, Blanca Majem, Laura Devis, Lara Lapyckyj, María José Besso, Marta Llauradó, María Florencia Abascal, María Laura Matos, Lucia Lanau, Josep Castellví, José Luis Sánchez, Asunción Pérez Benavente, Antonio Gil-Moreno, Jaume Reventós, Anna Santamaria Margalef, Marina Rigau, Mónica Hebe Vazquez-Levin
Ovarian cancer (OC) is the fifth cancer death cause in women worldwide. The malignant nature of this disease stems from its unique dissemination pattern. Epithelial-to-mesenchymal transition (EMT) has been reported in OC and downregulation of Epithelial cadherin (E-cadherin) is a hallmark of this process. However, findings on the relationship between E-cadherin levels and OC progression, dissemination and aggressiveness are controversial. In this study, the evaluation of E-cadherin expression in an OC tissue microarray revealed its prognostic value to discriminate between advanced- and early-stage tumors, as well as serous tumors from other histologies...
2017: PloS One
https://www.readbyqxmd.com/read/28933823/two-color-810-nm-sted-nanoscopy-of-living-cells-with-endogenous-snap-tagged-fusion-proteins
#11
Alexey N Butkevich, Haisen Ta, Michael Ratz, Stefan Stold, Stefan Jakobs, Vladimir N Belov, Stefan W Hell
A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell superresolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP-tag, together with a non-covalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.
September 21, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28933253/the-role-of-p53-microrna-200-moesin-axis-in-invasion-and-drug-resistance-of-breast-cancer-cells
#12
Farheen Alam, Fatima Mezhal, Hussain El Hasasna, Vidhya A Nair, S R Aravind, Maha Saber Ayad, Ahmed El-Serafi, Wael M Abdel-Rahman
This study aimed to analyze the expression of microRNAs in relation to p53 status in breast cancer cells and to delineate the role of Moesin in this axis. We used three isogenic breast carcinoma cell lines MCF7 (with wild-type p53), 1001 (MCF7 with mutated p53), and MCF7-E6 (MCF7 in which p53 function was disrupted). MicroRNA expression was analyzed using microarray analysis and confirmed by real-time polymerase chain reaction. The 1001 clone with mutant p53 showed 22 upregulated and 25 downregulated microRNAs...
September 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28931215/berberine-could-inhibit-thyroid-carcinoma-cells-by-inducing-mitochondrial-apoptosis-g0-g1-cell-cycle-arrest-and-suppressing-migration-via-pi3k-akt-and-mapk-signaling-pathways
#13
Liang Li, Xingchun Wang, Rampersad Sharvan, Jingyang Gao, Shen Qu
Berberine, an important natural isoquinoline alkaloid from traditional Chinese medicine, is reported to exhibit multiple pharmacological properties, including anti-microbial, anti-diabetes, anti-obesity, anti-inflammatory and anti-carcinogenic activities. Although studies have shown that a wide range of carcinoma cells could be inhibited by berberine, few studies involved thyroid carcinoma. We therefore examined the effect of berberine on papillary thyroid carcinoma (PTC, the most common subtype) and anaplastic thyroid carcinoma (ATC, the most malignant and aggressive subtype)...
September 16, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28931214/baicalin-alleviates-radiation-induced-epithelial-mesenchymal-transition-of-primary-type-ii-alveolar-epithelial-cells-via-tgf-%C3%AE-and-erk-gsk3%C3%AE-signaling-pathways
#14
Jinhua Lu, Yazhen Zhong, Zechen Lin, Xianlei Lin, Zhaohui Chen, Xuping Wu, Nan Wang, Haiqiao Zhang, Siyu Huang, Yuan Zhu, Yuanyuan Wang, Shengyou Lin
BACKGROUND: Radiation therapy is commonly used to treat thoracic malignancies. However, it may lead to severe lung pneumonitis and ultimately fibrosis. Irradiation has been reported to increase epithelial-mesenchymal transition (EMT) of type II alveolar epithelial cells (AEC), which play an important role in pulmonary fibrosis. The transforming growth factor-β (TGF-β) and ERK/glycogen synthase kinase 3β (GSK3β) pathways are critically involved in radiation-induced EMT. In the present study, we investigated whether baicalin was a novel therapeutic candidate for radiation-induced EMT in type II AEC...
September 16, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28928801/ginsenoside-rb1-inhibits-hypoxia-induced-epithelial-mesenchymal-transition-in-ovarian-cancer-cells-by-regulating-microrna-25
#15
Dan Liu, Ting Liu, Yue Teng, Wei Chen, Le Zhao, Xu Li
Metastasis frequently occurs in advanced ovarian cancer, which not only leads to substantial mortality but also becomes a major challenge to effective treatment. Epithelial-mesenchymal transition (EMT) is a key mechanism facilitating cancer metastasis. Targeting the EMT process with more efficacious and less toxic agents to prevent metastasis is of significant therapeutic value for ovarian cancer treatment. The anti-EMT function and mechanism of ginsenoside Rb1, a monomer composition extracted from the traditional Chinese herb Panax ginseng or P...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28928657/colocynth-extracts-prevent-epithelial-to-mesenchymal-transition-and-stemness-of-breast-cancer-cells
#16
Kaushik Chowdhury, Ankit Sharma, Suresh Kumar, Gyanesh K Gunjan, Alo Nag, Chandi C Mandal
Modern treatment strategies provide better overall survival in cancer patients, primarily by controlling tumor growth. However, off-target and systemic toxicity, tumor recurrence, and resistance to therapy are still inadvertent hurdles in current treatment regimens. Similarly, metastasis is another deadly threat to patients suffering from cancer. This has created an urgent demand to come up with new drugs having anti-metastatic potential and minimum side effects. Thus, this study was aimed at exploring the anti-proliferative and anti-metastatic potential of colocynth medicinal plant...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28927842/synthesis-and-preparation-of-biodegradable-hybrid-dextran-hydrogel-incorporated-with-biodegradable-curcumin-nanomicelles-for-full-thickness-wound-healing
#17
Mona Alibolandi, Marzieh Mohammadi, Seyed Mohammad Taghdisi, Khalil Abnous, Mohammad Ramezani
There is a clinical need for a novel, more efficient therapy for full thickness wound healing. In the current study, curcumin encapsulated PEG-PLA [poly(lactide)-block-poly(ethylene glycol)] nanomicelles were incorporated into dextran hydrogel for a full thickness dermal wound healing application. To assess the application of the hydrogel as a therapeutic wound dressing, its morphology, swelling pattern, kinetics of degradation, and capacity to control curcumin release were evaluated. It was found that the prepared hybrid hydrogel had acceptable biocompatibility, incorporation capacity of curcumin nanomicelles, and mechanical properties...
September 16, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28927046/celastrus-orbiculatus-extract-suppresses-the-epithelial-mesenchymal-transition-by-mediating-cytoskeleton-rearrangement-via-inhibition-of-the-cofilin-1-signaling-pathway-in-human-gastric-cancer
#18
Haibo Wang, Hao Gu, Jun Feng, Yayun Qian, Lin Yang, Feng Jin, Xuanyi Wang, Jue Chen, Youyang Shi, Songhua Lu, Min Zhao, Yanqing Liu
Celastrus orbiculatus is a traditional medicinal plant used in the anti-inflammatory and analgesic treatment of various diseases. A previous study demonstrated that ethyl acetate extract of C. orbiculatus (COE) exhibited significant antitumor effects. However, studies concerning the effects and mechanism of COE in terms of suppressing the epithelial-mesenchymal transition (EMT) in human gastric adenocarcinoma cells have not been performed at present. The present study hypothesized that COE may inhibit EMT in gastric adenocarcinoma cells by regulating cell cytoskeleton rearrangement...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28926892/-effect-and-mechanism-of-silibinin-on-the-inhibition-of-alk-positive-nsclc-cells-by-sensitizing-crizotinib
#19
C C Lin, C H Lu, Y H Pan, L Jiao, H Y Chen, L Li, Y He
Objective: To explore the synergistic effect of silibinin combined with crizotinib on anaplastic lymphoma kinase positive (ALK+ ) non-small cell lung cancer (NSCLC) cells and its mechanism. Methods: H2228 and H3122 cells were treated with silibinin, crizotinib alone or in combination. Cell proliferation was measured by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and colony formation assay. Migration or invasion ability was tested by wound healing assay or transwell assay, respectively...
September 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28926138/tnfaip8-regulates-hippo-pathway-through-interacting-with-lats1-to-promote-cell-proliferation-and-invasion-in-lung-cancer
#20
Yong Han, ZhongPing Tang, Yue Zhao, Qingchang Li, Enhua Wang
TNFAIP8 is associated with prognosis of several human malignancies. However, the molecular mechanism of TNFAIP8 in lung cancer remains unknown. In our study, we found TNFAIP8 could enhance TEAD luciferase activity and inhibits the activity of Hippo pathway. TNFAIP8 also increased cyclin D1, CDK6 and decreased p27 in lung cancer cells. In addition, TNFAIP8 increased total YAP protein and promoted nuclear localization of YAP. More importantly, YAP depletion blocked the role of TNFAIP8 on cell cycle-related proteins and TEAD luciferase activity, revealing that TNFAIP8 regulates Hippo pathway in a YAP-dependend manner...
September 19, 2017: Molecular Carcinogenesis
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