Ying Li, Xiaofang Cheng, Miao Li, Yingli Wang, Tao Fu, Zixuan Zhou, Yaru Wang, Xinyu Gong, Xiaolong Xu, Jianping Liu, Lifeng Pan
Syntaxin17, a key autophagosomal N -ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein, can associate with ATG8 family proteins SNAP29 and VAMP8 to facilitate the membrane fusion process between the double-membraned autophagosome and single-membraned lysosome in mammalian macroautophagy. However, the inherent properties of Syntaxin17 and the mechanistic basis underlying the interactions of Syntaxin17 with its binding proteins remain largely unknown. Here, using biochemical, NMR, and structural approaches, we systemically characterized Syntaxin17 as well as its interactions with ATG8 family proteins, SNAP29 and VAMP8...
August 19, 2020: Proceedings of the National Academy of Sciences of the United States of America