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https://www.readbyqxmd.com/read/28222186/alpk1-controls-tifa-traf6-dependent-innate-immunity-against-heptose-1-7-bisphosphate-of-gram-negative-bacteria
#1
Milica Milivojevic, Anne-Sophie Dangeard, Christoph Alexander Kasper, Therese Tschon, Mario Emmenlauer, Claudine Pique, Pamela Schnupf, Julie Guignot, Cécile Arrieumerlou
During infection by invasive bacteria, epithelial cells contribute to innate immunity via the local secretion of inflammatory cytokines. These are directly produced by infected cells or by uninfected bystanders via connexin-dependent cell-cell communication. However, the cellular pathways underlying this process remain largely unknown. Here we perform a genome-wide RNA interference screen and identify TIFA and TRAF6 as central players of Shigella flexneri and Salmonella typhimurium-induced interleukin-8 expression...
February 21, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28221718/quantitative-analysis-of-shigella-flexneri-protein-expression-under-acid-stress
#2
Kaiwen Yu, Zhen Wang, Fan Zhou, Jiezhang Jiang, Yanhua Liu, Mo Hu, Xiaoyun Liu
As an important foodborne pathogen, Shigella flexneri can cause widespread enteric infection with bacteria as few as hundreds. This is, at least in part, attributed to its robust anti-acid strategies because passage through the highly acidic human digestive tract is a prerequisite for successful bacterial infection. Nevertheless, our understanding of these mechanisms and the impact of acid stress on Shigella protein expression still remains largely incomplete. Herein we conducted a proteomic survey of Shigella spp...
February 21, 2017: Proteomics
https://www.readbyqxmd.com/read/28219825/first-report-on-whole-genome-shotgun-sequences-of-23-biochemically-indistinguishable-clinical-shigella-isolates
#3
Dhiviya Prabaa Muthuirulandi Sethuvel, Naveen Kumar Devanga Ragupathi, Shalini Anandan, Valsan Philip Verghese, Balaji Veeraraghavan
Shigella spp. are a major diarrhoeal disease pathogen worldwide and can cause considerable morbidity and mortality. Notably, limited genome data are available for serogroups/sub-serogroups of Shigella. Here we report the whole-genome shotgun sequences of 23 non-typeable Shigella from stool specimens that biochemically resembled Shigella spp. but were non-typeable with Shigella-specific antisera.
February 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28218003/virulotyping-of-shigella-spp-isolated-from-pediatric-patients-in-tehran-iran
#4
Reza Ranjbar, Masomeh Bolandian, Payam Behzadi
Shigellosis is a considerable infectious disease with high morbidity and mortality among children worldwide. In this survey the prevalence of four important virulence genes including ial, ipaH, set1A, and set1B were investigated among Shigella strains and the related gene profiles identified in the present investigation, stool specimens were collected from children who were referred to two hospitals in Tehran, Iran. The samples were collected during 3 years (2008-2010) from children who were suspected to shigellosis...
February 20, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28207509/relationship-between-drug-resistance-and-the-clustered-regularly-interspaced-short-palindromic-repeat-associated-protein-genes-cas1-and-cas2-in-shigella-from-giant-panda-dung
#5
Lu Ren, Lin-Hua Deng, Ri-Peng Zhang, Cheng-Dong Wang, De-Sheng Li, Li-Xin Xi, Zhen-Rong Chen, Rui Yang, Jie Huang, Yang-Ru Zeng, Hong-Lin Wu, San-Jie Cao, Rui Wu, Yong Huang, Qi-Gui Yan
BACKGROUND: To detect drug resistance in Shigella obtained from the dung of the giant panda, explore the factors leading to drug resistance in Shigella, understand the characteristics of clustered, regularly interspaced, short, palindromic repeats (CRISPR), and assess the relationship between CRISPR and drug resistance. METHODS: We collected fresh feces from 27 healthy giant pandas in the Giant Panda Conservation base (Wolong, China). We identified the strains of Shigella in the samples by using nucleotide sequence analysis...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28207293/prevalence-of-multidrug-resistant-bacteria-from-u-s-grown-and-imported-fresh-produce-retailed-in-chain-supermarkets-and-ethnic-stores-of-davidson-county-tennessee
#6
Siqin Liu, Agnes Kilonzo-Nthenge
The aim of this study was to determine whether U.S.-grown and imported fresh produce retailed in ethnic stores and chain supermarkets was a reservoir of antibiotic-resistant bacteria. A total of 360 (129 imported and 231 U.S.-grown) samples of fresh produce were purchased from retail stores and analyzed for Enterobacteriaceae , including three pathogenic bacteria ( Escherichia coli O157:H7, Shigella , and Salmonella ), using standard methods. Presumptive pathogenic isolates were confirmed using PCR. The mean Enterobacteriaceae counts for imported produce were 6...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28199151/antimicrobial-resistance-percentages-of-salmonella-and-shigella-in-seafood-imported-to-jordan-higher-percentages-and-more-diverse-profiles-in-shigella
#7
Mohammad M Obaidat, Alaa E Bani Salman
This study determined the prevalence and antimicrobial resistance of human-specific ( Shigella spp.) and zoonotic ( Salmonella enterica ) foodborne pathogens in internationally traded seafood. Sixty-four Salmonella and 61 Shigella isolates were obtained from 330 imported fresh fish samples from Egypt, Yemen, and India. The pathogens were isolated on selective media, confirmed by PCR, and tested for antimicrobial resistance. Approximately 79 and 98% of the Salmonella and Shigella isolates, respectively, exhibited resistance to at least one antimicrobial, and 8 and 49% exhibited multidrug resistance (resistance to three or more antimicrobial classes)...
February 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28199144/attachment-of-13-types-of-foodborne-bacteria-to-jalape%C3%A3-o-and-serrano-peppers-and-antibacterial-effect-of-roselle-calyx-extracts-sodium-hypochlorite-colloidal-silver-and-acetic-acid-against-these-foodborne-bacteria-on-peppers
#8
Esmeralda Rangel-Vargas, Carlos A Gómez-Aldapa, Reyna N Falfan-Cortes, María L Rodríguez-Marín, Angélica Godínez-Oviedo, Otilio A Acevedo-Sandoval, Javier Castro-Rosas
Chili peppers are a very important crop in Mexico. However, these peppers have been associated with Salmonella infection outbreaks in the United States, and Salmonella and diarrheagenic Escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in Mexico. To decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. Roselle ( Hibiscus sabdariffa ) calyces have been reported to have an antimicrobial effect on pathogenic bacteria...
February 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28197924/sumoylation-as-an-integral-mechanism-in-bacterial-infection-and-disease-progression
#9
Chittur V Srikanth, Smriti Verma
Post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. Among these, particularly the modification with Small Ubiquitin-like Modifiers (SUMO) is being recognized as a pathway crucial for cell homeostasis and health. Understandably, bacterial pathogens intervene with the SUMO pathway of the host for ensuring successful infection. Among the bacterial pathogens known to target host sumoylation varied points of intervention are utilized. Majority of them including Salmonella Typhimurium, Shigella flexneri and Listeria monocytogenes target the E2 conjugating enzyme Ubc9...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28197578/chemical-characterization-of-the-glycated-myofibrillar-proteins-from-grass-carp-ctenopharyngodon-idella-and-their-impacts-on-the-human-gut-microbiota-in-vitro-fermentation
#10
Kaining Han, Ye Yao, Shiyuan Dong, Sun Jin, Hang Xiao, Haohao Wu, Mingyong Zeng
In this study, the chemical characterization of glycoconjugates of myofibrillar proteins from grass carp conjugated with glucose via Maillard reaction for up to 24 h of dry-heating was investigated, and their impacts on the microbial community in vitro human fecal fermentation were firstly evaluated by high-throughput sequencing technologies. The glycation greatly increased the furosine levels in glycoconjugates, which reached the maximum level (2.87 ± 0.08 mg per 100 mg protein) for 9 h of heating, and resulted in the structural changes of myofibrillar proteins based on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR) analysis...
February 15, 2017: Food & Function
https://www.readbyqxmd.com/read/28192710/rational-design-of-reversible-inhibitors-for-trehalose-6-phosphate-phosphatases
#11
Chunliang Liu, Debra Dunaway-Mariano, Patrick S Mariano
In some organisms, environmental stress triggers trehalose biosynthesis that is catalyzed collectively by trehalose 6-phosphate synthase, and trehalose 6-phosphate phosphatase (T6PP). T6PP catalyzes the hydrolysis of trehalose 6-phosphate (T6P) to trehalose and inorganic phosphate and is a promising target for the development of antibacterial, antifungal and antihelminthic therapeutics. Herein, we report the design, synthesis and evaluation of a library of aryl d-glucopyranoside 6-sulfates to serve as prototypes for small molecule T6PP inhibitors...
February 4, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28192483/development-of-a-high-throughput-method-to-evaluate-serum-bactericidal-activity-using-bacterial-atp-measurement-as-survival-readout
#12
Francesca Necchi, Allan Saul, Simona Rondini
Serum Bactericidal Activity (SBA) assay is the method of choice to evaluate the complement-mediated functional activity of both infection- and vaccine-induced antibodies. To perform a typical SBA assay, serial dilutions of sera are incubated with target bacterial strains and complement. The conventional SBA assay is based on plating on agar the SBA reaction mix and counting the surviving bacterial colony forming units (CFU) at each serum dilution. Even with automated colony counting, it is labor-intensive, time-consuming and not amenable for large-scale studies...
2017: PloS One
https://www.readbyqxmd.com/read/28188118/an-11-year-study-of-shigellosis-and-shigella-species-in-taiyuan-china-active-surveillance-epidemic-characteristics-and-molecular-serotyping
#13
Lifeng Zhao, Yanwen Xiong, Dequan Meng, Jiane Guo, Yiping Li, Lirong Liang, Rui Han, Yanqin Wang, Xiaofang Guo, Rui Wang, Ladi Zhang, Li Gao, Jitao Wang
A hospital-based surveillance of shigellosis was conducted in Taiyuan from 2005 to 2015. A total of 2655 stool cultures were collected from patients with diarrhea, 115 were identified as S. flexneri and 107 were S. sonnei. The highest infection rates were found among children under 5 years of age (34.2 %), and during the summer (61.0 %). ​Six serotypes were identified among S. flexneriisolates:1a, 2a, 2b, Xv, X and Y. Serotype 2a and Xv were the dominant serotypes in two periods, 2012-2015 and 2005-2008, respectively...
February 7, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28178108/a-hospital-based-case-control-study-of-diarrhea-in-children-in-shanghai
#14
Hailing Chang, Ling Zhang, Yanling Ge, Jiehao Cai, Xiangshi Wang, Zheng Huang, Jiayin Guo, Hao Xu, Zhen Gu, Hong Chen, Xuebin Xu, Mei Zeng
BACKGROUND: Prevention and control of childhood diarrhea is a global priority. We conducted a case-control study of childhood diarrhea in Shanghai. METHODS: We prospectively recruited diarrheal children in an outpatient setting. Non-diarrheal controls were individually matched to cases. Rotavirus, norovirus and bacterial pathogens were examined. Clinical and epidemiologic data were obtained at enrollment and follow-up. RESULTS: Potential pathogens identified in 680 diarrheal cases and 680 controls were rotavirus (19...
February 6, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28176523/glycomacropeptide-sustains-microbiota-diversity-and-promotes-specific-taxa-in-an-artificial-colon-model-of-elderly-gut-microbiota
#15
Alexandra Ntemiri, Fodhla Ni Chonchuir, Tom F O'Callaghan, Catherine Stanton, R Paul Ross, Paul W O'Toole
The potential of milk-derived glycomacropeptide (GMP) and lactose for modulating the human gut microbiota of older people, in whom loss of diversity correlates with inferior health, was investigated. We used an in vitro batch fermentation (artificial colon model) to simulate colonic fermentation processes of two GMP products, i.e. a commercially available GMP concentrate and a semi-purified GMP concentrate, and lactose. Faecal samples were collected from healthy and frail older people. Samples were analysed by Illumina Miseq sequencing of rRNA gene amplicons...
February 8, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28152988/identification-and-antimicrobial-resistance-patterns-of-bacterial-enteropathogens-from-children-aged-0-59-months-at-the-university-teaching-hospital-lusaka-zambia-a-prospective-cross-sectional-study
#16
Harriet Chiyangi, John B Muma, Sydney Malama, Joel Manyahi, Ahmed Abade, Geoffrey Kwenda, Mecky I Matee
BACKGROUND: Bacterial diarrhoeal disease is among the most common causes of mortality and morbidity in children 0-59 months at the University Teaching Hospital in Lusaka, Zambia. However, most cases are treated empirically without the knowledge of aetiological agents or antimicrobial susceptibility patterns. The aim of this study was, therefore, to identify bacterial causes of diarrhoea and determine their antimicrobial susceptibility patterns in stool specimens obtained from the children at the hospital...
February 2, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28149489/a-comparison-of-antibiotic-disks-from-different-sources-on-quicolor-and-mueller-hinton-agar-media-in-evaluation-of-antibacterial-susceptibility-testing
#17
Neda Saffari, Siavosh Salmanzadeh-Ahrabi, Ahya Abdi-Ali, Mohsen Rezaei-Hemami
BACKGROUND AND OBJECTIVES: Antibacterial susceptibility testing of clinical bacterial isolates through disk diffusion method plays a major role in antibacterial treatment. One of the main factors affecting the result of these tests is the type, structure and quality of the disks. The main objective of this study was to compare the agreement of antibiotic disks originated from three companies on Quicolor and Mueller-Hinton agar. MATERIALS AND METHODS: Quicolor and Mueller-Hinton agar media were used in disk diffusion method...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149488/multi-locus-sequence-type-analysis-of-shigellas-pp-isolates-from-tehran-iran
#18
Shadi Shahsavan, Maliheh Nobakht, Abdolaziz Rastegar-Lari, Parviz Owlia, Bita Bakhshi
BACKGROUND AND OBJECTIVES: Strains of Shigella spp. can cause shigellosis, or bacillary dysentery. that is a public health problem worldwide. The aim of this study was to describe the population structure and genetic relatedness of multidrug resistant S. sonnei and S. flexneri isolated during a one year period from children with diarrhea in Tehran, Iran. MATERIALS AND METHODS: A total of 70 Shigella spp. were detected during the study period. Twenty MDR isolates of Shigella spp...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28146281/flow-cytometry-based-method-to-detect-escherichia-coli-and-shigella-spp-using-16s-rrna-based-probe
#19
Yong Xue, Jon G Wilkes, Ted J Moskal, Anna J Williams, Willie M Cooper, Dan A Buzatu
Detection of microbial contamination in foods before they go on to the market can help prevent the occurrence of foodborne illness outbreaks. Current methods for the detection of Escherichia coli are limited by time-consuming procedures, which include multiple culture incubation steps, and require several days to get results. This unit describes the development of an improved rapid flow-cytometry-based detection method that has greater sensitivity and specificity. This method requires less time-to-results (TTR) and can detect a small number of E...
February 1, 2017: Current Protocols in Toxicology
https://www.readbyqxmd.com/read/28134784/sibling%C3%A2-srna%C3%A2-ryfa1%C3%A2-influences%C3%A2-shigella%C3%A2-dysenteriae-pathogenesis
#20
Megan E Fris, William H Broach, Sarah E Klim, Peter W Coschigano, Ronan K Carroll, Clayton C Caswell, Erin R Murphy
Small regulatory RNAs (sRNAs) of Shigella dysenteriae and other pathogens are vital for the regulation of virulence-associated genes and processes. Here, we characterize RyfA1, one member of a sibling pair of sRNAs produced by S. dysenteriae. Unlike its nearly identical sibling molecule, RyfA2, predicted to be encoded almost exclusively by non-pathogenic species, the presence of a gene encoding RyfA1, or a RyfA1-like molecule, is strongly correlated with virulence in a variety of enteropathogens. In S. dysenteriae, the overproduction of RyfA1 negatively impacts the virulence-associated process of cell-to-cell spread as well as the expression of ompC, a gene encoding a major outer membrane protein important for the pathogenesis of Shigella...
January 26, 2017: Genes
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