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https://www.readbyqxmd.com/read/29457530/a-new-insight-into-the-classification-of-dusky-thrush-complex-bearings-on-the-phylogenetic-relationships-within-the-turdidae
#1
Yuanqiu Dong, Bo Li, Lizhi Zhou
Dusky thrush complex comprises of two sister species breeding in SC Siberia, which is the member of thrush Turdus from Turdidae. The phylogenetic resolution of Dusky thrush complex remains controversial, and a detailed research is still necessary. In this research, we determined the complete mtDNAs of both species, and estimated phylogenetic trees based on the mtDNA alignment of these and 21 other Turdidae species, to clarify the taxa status of the Dusky thrush complex. The squenced lengths of these three mitochondrial genomes were 16,737, 16,788 and 16,750 bp...
February 18, 2018: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/29453960/synaptic-dysfunction-and-altered-excitability-in-c9orf72-als-ftd
#2
Alexander Starr, Rita Sattler
Amyotrophic lateral sclerosis (ALS) is characterized by a progressive degeneration of upper and lower motor neurons, resulting in fatal paralysis due to denervation of the muscle. Due to genetic, pathological and symptomatic overlap, ALS is now considered a spectrum disease together with frontotemporal dementia (FTD), the second most common cause of dementia in individuals under the age of 65. Interestingly, in both diseases, there is a large prevalence of RNA binding proteins (RBPs) that are mutated and considered disease-causing, or whose dysfunction contribute to disease pathogenesis...
February 14, 2018: Brain Research
https://www.readbyqxmd.com/read/29446201/transposable-elements-shape-the-human-proteome-landscape-via-formation-of-cis-acting-upstream-open-reading-frames
#3
Shohei Kitano, Hikaru Kurasawa, Yasunori Aizawa
Transposons are major drivers of mammalian genome evolution. To obtain new insights into the contribution of transposons to the regulation of protein translation, we here examined how transposons affected the genesis and function of upstream open reading frames (uORFs), which serve as cis-acting elements to regulate translation from annotated ORFs (anORFs) located downstream of the uORFs in eukaryotic mRNAs. Among 39,786 human uORFs, 3,992 had ATG trinucleotides of a transposon origin, termed "transposon-derived upstream ATGs" or TuATGs...
February 15, 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/29441798/investigating-mirna-661-and-atg4-b-mrna-expression-as-potential-biomarkers-for-hepatocellular-carcinoma
#4
Mahmoud A Ali, Marwa Matboli, Nashwa El-Khazragy, Osama Saber, Sarah El-Nakeep, Hana M Abdelzaher, Ayman El-Sayed Shafei, Randa Mostafa
AIM: We aimed to examine the statistical association between serum expression of miRNA 661 (miR-661) and ATG-4B mRNA and hepatocellular carcinoma (HCC) based on in silico data analysis followed by clinical validation. PATIENTS & METHODS: Quantitative reverse-transcriptase real-time PCR was used to examine the expression of miR-661 and ATG-4B mRNA in the sera of HCC patients versus control. RESULTS: The expression of miR-661 and ATG-4B mRNA was positive in 97...
February 14, 2018: Biomarkers in Medicine
https://www.readbyqxmd.com/read/29435650/targeted-knock-in-of-creer-t2-in-zebrafish-using-crispr-cas9
#5
Gokul Kesavan, Juliane Hammer, Stefan Hans, Michael Brand
New genome-editing approaches, such as the CRISPR/Cas system, have opened up great opportunities to insert or delete genes at targeted loci and have revolutionized genetics in model organisms like the zebrafish. The Cre-loxp recombination system is widely used to activate or inactivate genes with high spatial and temporal specificity. Using a CRISPR/Cas9-mediated knock-in strategy, we inserted a zebrafish codon-optimized CreER T2 transgene at the otx2 gene locus to generate a conditional Cre-driver line. We chose otx2 as it is a patterning gene of the anterior neural plate that is expressed during early development...
February 12, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29434791/efficacy-and-safety-of-combined-immunosuppressive-therapy-plus-umbilical-cord-blood-infusion-in-severe-aplastic-anemia-patients-a-cohort-study
#6
Xiu Luo, Huina Lu, Bing Xiu, Hao Wu, Bing Li, Ping Li, Yuhua Chen, Lili Zhou, Wenjun Zhang, Yan Dong, Aibin Liang, Yi Ding
The present study aimed to evaluate the efficacy and safety of combined immunosuppressive therapy (IST) plus umbilical cord blood infusion (UCBI) in severe aplastic anemia (SAA) patients. A total of 68 patients with SAA were enrolled in the current prospective cohort study and divided into the IST (n=35; positive control) and IST+UCBI (n=33; experimental) groups according to the treatment conditions. Patients in the IST group were treated with rabbit antithymocyte globulin (r-ATG) at a dose of 2.5 mg/kg through intravenous infusion once a day for five days...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29432302/allogeneic-matched-related-donor-bone-marrow-transplantation-for-pediatric-patients-with-severe-aplastic-anemia-using-low-dose-cyclophosphamide-atg-plus-fludarabine
#7
Chayamon Takpradit, Susan E Prockop, Nancy A Kernan, Andromachi Scaradavou, Kevin Curran, Julianne Ruggiero, Nicole Zakak, Richard J O'Reilly, Farid Boulad
BACKGROUND: The combination of cyclophosphamide (CY) and antithymocyte globulin (ATG) has been used as a standard conditioning regimen for matched related donor transplantation in patients with severe aplastic anemia. PROCEDURE: To decrease the regimen-related toxicity while maintaining appropriate engraftment and survival rates, fludarabine (FLU) was added to the regimen. Four pediatric patients received matched related donor bone marrow transplantation with CY (50 mg/kg×2) (instead of the 50 mg/kg×4 standard dosing), equine ATG (30 mg/kg×3), with the addition of FLU (30 mg/m×4)...
February 9, 2018: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/29426829/impaired-thymopoiesis-predicts-for-a-high-risk-of-severe-infections-after-reduced-intensity-conditioning-without-anti-thymocyte-globulin-in-double-umbilical-cord-blood-transplantation
#8
Lucia E Duinhouwer, Nick Beije, Bronno van der Holt, Anita Rijken-Schelen, Cor H Lamers, Judith Somers, Eric Braakman, Jan J Cornelissen
Umbilical cord blood stem cell transplantation (UCBT) is associated with retarded hematopoietic recovery and immune reconstitution and a high infection-related morbidity and mortality, especially after conditioning including anti-thymocyte globulin (ATG). However, data on immune recovery, incidence of infections, and outcome in double UCBT (dUCBT) recipients receiving an ATG-free reduced intensity conditioning (RIC) are lacking. In this study, recovery of lymphocyte subsets, thymopoiesis, and its association with severe infections and clinical outcome was assessed in a group of 55 recipients of a dUCBT ATG-free RIC regimen...
February 9, 2018: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/29416600/impaired-autophagic-flux-and-its-related-inflammation-in-patients-with-adult-onset-still-s-disease
#9
Chia-Wei Hsieh, Chun-Yu Chang, Yi-Ming Chen, Hsin-Hua Chen, Wei-Ting Hung, Ning-Rong Gung, Shiow-Jiuan Wey, Der-Yuan Chen
The pathogenic role of autophagic immune regulation in adult-onset Still's disease (AOSD) is unclear. We investigated the relative levels of autophagy in AOSD patients and healthy controls, its association with disease activity or course, and the change in autophagy after 6 months of therapy. Autophagosome levels were determined from the mean fluorescence intensity of autophagosomotropic dye incorporated into circulating immune cells. The fluorescent signal from lymphocytes, monocytes, and granulocytes from AOSD patients was greater than from controls...
January 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29415633/danazol-increases-t-regulatory-cells-in-patients-with-aplastic-anemia
#10
Harshit Khurana, Pankaj Malhotra, Man Updesh Sachdeva, Neelam Varma, Parveen Bose, Uday Yanamandra, Subhash Varma, Alka Khadwal, Deepesh Lad, Gaurav Prakash
OBJECTIVES: Danazol is an attenuated androgen and is used in the treatment of aplastic anemia (AA) in resource constraint settings. We chose to study the role of CD4+ CD25high CD127low FoxP3+ T regulatory cells (T-regs) in the pathophysiology of AA and their response to treatment with Danazol alone or in combination with immunosuppressive treatment (IST). METHODS: T-regs' percentages of 25 acquired idiopathic AA patients and 25 healthy controls who completed study protocol were analyzed by performing multicolor flowcytometry on peripheral blood samples...
February 8, 2018: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/29413888/membrane-trafficking-in-autophagy
#11
Kristiane Søreng, Thomas P Neufeld, Anne Simonsen
Macroautophagy is an intracellular pathway used for targeting of cellular components to the lysosome for their degradation and involves sequestration of cytoplasmic material into autophagosomes formed from a double membrane structure called the phagophore. The nucleation and elongation of the phagophore is tightly regulated by several autophagy-related (ATG) proteins, but also involves vesicular trafficking from different subcellular compartments to the forming autophagosome. Such trafficking must be tightly regulated by various intra- and extracellular signals to respond to different cellular stressors and metabolic states, as well as the nature of the cargo to become degraded...
2018: International Review of Cell and Molecular Biology
https://www.readbyqxmd.com/read/29410282/osmotic-stress-induced-toxicity-exacerbates-parkinson-s-associated-effects-via-dysregulation-of-autophagy-in-transgenic-c-elegans-model
#12
Pooja Jadiya, Snober S Mir, Aamir Nazir
The accumulation of aggregate-prone proteins is a major representative of many neurological disorders, including Parkinson's disease (PD) wherein the cellular clearance mechanisms, such as the ubiquitin-proteasome and autophagy pathways are impaired. PD, known to be associated with multiple genetic and environmental factors, is characterized by the aggregation of α-synuclein protein and loss of dopaminergic neurons in midbrain. This disease is also associated with other cardiovascular ailments. Herein, we report our findings from studies on the effect of hyper and hypo-osmotic induced toxicity representing hyper and hypotensive condition as an extrinsic epigenetic factor towards modulation of Parkinsonism, using a genetic model Caenorhabditis elegans (C...
February 2, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29407753/schisandrin-c-enhances-odontoblastic-differentiation-through-autophagy-and-mitochondrial-biogenesis-in-human-dental-pulp-cells
#13
Jyoti Shrestha Takanche, Jeong-Seok Kim, Ji-Eun Kim, S-H Han, Ho-Keun Yi
OBJECTIVE: To investigate the role of Schisandrin C in odontoblastic differentiation, and its relations between autophagy and mitochondrial biogenesis in human dental pulp cells (HPDCs). DESIGN: Fresh third molars were used, and cultured for HDPCs. Western blotting technique, Alizarin red S staining, alkaline phosphatase (ALP) activity, and confocal microscopy were used to detect autophagy, mitochondrial biogenesis, and odontoblastic differentiation. To understand the mechanism of Schisandrin C, the HDPCs were treated with lipopolysaccharide (LPS), autophagy and heme oxygenase-1 (HO-1) inhibitors: 3-Methyladenine (3-MA) and Zinc protoporphyrin IX (ZnPP), respectively...
January 30, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29403023/allogenic-peripheral-stem-cell-transplantation-from-hla-matched-related-donors-for-adult-sickle-cell-disease-remarkable-outcomes-from-a-single-center-trial
#14
H Ozdogu, C Boga, M Yeral, I Kozanoglu, C Gereklioglu, P Aytan, M Kasar, S Asma, N Buyukkurt, S Solmaz, A Korur, C Sariturk
Adult patients with sickle cell disease (SCD) are highly susceptible to stem cell transplant complications, including drug toxicity, graft versus host disease (GVHD), and graft rejection due to SCD-related tissue damage, endothelial activation, and inflammation. The scarcity of compatible stem cells for transplantation further limits treatment options, with only 43 cases of adult allogeneic peripheral blood stem cell transplantation (allo-PSCT) from human leukocyte antigen (HLA)-identical sibling donors reported in the international registry for the period 1986-2013...
February 5, 2018: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/29400609/adipose-tissue-autophagy-related-gene-expression-is-associated-with-glucometabolic-status-in-human-obesity
#15
Qing Xu, Edwin C M Mariman, Nadia J T Roumans, Roel G Vink, Gijs H Goossens, Ellen E Blaak, Johan W E Jocken
Adipose tissue autophagy (AT) is associated with human obesity and increased metabolic risk. Recent findings establish a role for autophagy in lipid metabolism. Here, we compared the expression of autophagy-related and lipolysis genes in human abdominal subcutaneous AT (SCAT) in overweight/obese subjects (n = 17) with or without impaired glucose tolerance in comparison with lean normal glucose tolerant individuals (n = 9), and investigated the association between AT autophagy and lipolysis. Human multipotent adipose-derived stem cells (hMADS) were used to investigate the effect of pharmacological HSL inhibition on changes in the autophagic flux...
February 5, 2018: Adipocyte
https://www.readbyqxmd.com/read/29391515/heterologous-pathway-assembly-reveals-molecular-steps-of-fungal-terreic-acid-biosynthesis
#16
Chuixing Kong, Hezhou Huang, Ying Xue, Yiqi Liu, Qiangqiang Peng, Qi Liu, Qin Xu, Qiaoyun Zhu, Ying Yin, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai
Terreic acid is a potential anticancer drug as it inhibits Bruton's tyrosine kinase; however, its biosynthetic molecular steps remain unclear. In this work, the individual reactions of terreic acid biosynthesis were determined by stepwise pathway assembly in a heterologous host, Pichia pastoris, on the basis of previous knockout studies in a native host, Aspergillus terreus. Polyketide synthase AtX was found to catalyze the formation of partially reduced polyketide 6-methylsalicylic acid, followed by 3-methylcatechol synthesis by salicylate 1-monooxygenase AtA-mediated decarboxylative hydroxylation of 6-methylsalicylic acid...
February 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29390584/an-intracardiac-accessory-thyroid-gland-mimicking-cardiac-tumor-a-case-report-and-literature-review
#17
Lin-Jing Wang, Chun-Mei Liu, Xin Chen, Li Zhang, Hong-Wei Zhou
RATIONALE: An accessory thyroid gland (ATG) in the right ventricle is an extremely rare condition. Described herein are histological findings of ATG in the right ventricle found in a patient with a normal cervical thyroid gland. PATIENT CONCERNS: A 53-year-old woman was referred to our hospital after experiencing intermittent precordial pain for 2 years. DIAGNOSES: The mass in the right ventricle was diagnosed pathologically as ATG. INTERVENTIONS: Complete excision was performed because of the patient's intermittent precordial pain and to exclude the possibility of malignancy...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29390157/vacuolar-protein-degradation-via-autophagy-provides-substrates-to-amino-acid-catabolic-pathways-as-an-adaptive-response-to-sugar-starvation-in-arabidopsis-thaliana
#18
Takaaki Hirota, Masanori Izumi, Shinya Wada, Amane Makino, Hiroyuki Ishida
The vacuolar lytic degradation of proteins releases free amino acids (AAs) that plants can use instead of sugars for respiratory energy production. Autophagy is a major cellular process leading to the transport of proteins into the vacuole for degradation. Here, we examine the contribution of autophagy to the AA metabolism response to sugar starvation in mature leaves of Arabidopsis thaliana. During sugar starvation arising from the exposure of wild-type (WT) plants to darkness, autophagic transport of chloroplast stroma, which contains most of the proteins in a leaf, into the vacuolar lumen was induced within two days...
January 30, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29386198/effect-of-non-permissive-hla-dpb1-mismatches-after-unrelated-allogeneic-transplantation-with-in-vivo-t-cell-depletion
#19
Betül Oran, Rima M Saliba, Yudith Carmazzi, Marcos de Lima, Gabriela Rondon, Sairah Ahmed, Amin Alousi, Borje S Andersson, Paolo Anderlini, Michelle Alvarez, Qasier Bashir, Stefan Ciurea, Marcelo Fernandez-Vina, Chitra Hosing, Partow Kebriaei, Martin Korbling, Pedro Cano, Issa Khouri, David Marin, Yago Nieto, Amanda Olson, Uday Popat, Katy Rezvani, Muzaffar Qazilbash, Elizabeth J Shpall, Richard E Champlin, Kai Cao
We investigated the impact of donor-recipient HLA-DPB1 matching on outcomes of allogeneic hematopoietic stem cell transplantation with in vivo T cell depletion using ATG for patients with hematological malignancies. All donor-recipient pairs had high resolution typing for HLA-A, -B, -C, -DRB1, -DQB1, -DPB1 and -DRB3/4/5 and were matched at HLA-A, -B, -C and -DRB1. HLA-DPB1 mismatches were categorized by immunogenicity of the DPB1 matching using the DPB T-cell epitope tool. Of 1004 donor-recipient pairs, 210 (21%) were DPB1 matched, 443 (44%) had permissive mismatches, 184 (18%) non-permissive mismatches, in graft versus host (GvH) direction and 167 (17%) non-permissive mismatches in host versus graft (HvG) direction...
January 31, 2018: Blood
https://www.readbyqxmd.com/read/29383466/structure-based-drug-design-synthesis-and-biological-assays-of-p-falciparum-atg3-atg8-protein-protein-interaction-inhibitors
#20
Stefania Villa, Laura Legnani, Diego Colombo, Arianna Gelain, Carmen Lammi, Daniele Bongiorno, Denise P Ilboudo, Kellen E McGee, Jürgen Bosch, Giovanni Grazioso
The proteins involved in the autophagy (Atg) pathway have recently been considered promising targets for the development of new antimalarial drugs. In particular, inhibitors of the protein-protein interaction (PPI) between Atg3 and Atg8 of Plasmodium falciparum retarded the blood- and liver-stages of parasite growth. In this paper, we used computational techniques to design a new class of peptidomimetics mimicking the Atg3 interaction motif, which were then synthesized by click-chemistry. Surface plasmon resonance has been employed to measure the ability of these compounds to inhibit the Atg3-Atg8 reciprocal protein-protein interaction...
January 30, 2018: Journal of Computer-aided Molecular Design
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