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https://www.readbyqxmd.com/read/28630811/development-of-a-branched-radio-frequency-ion-trap-for-electron-based-dissociation-and-related-applications
#1
Takashi Baba, J Larry Campbell, J C Yves Le Blanc, Paul R S Baker, James W Hager, Bruce A Thomson
Collision-induced dissociation (CID) is the most common tool for molecular analysis in mass spectrometry to date. However, there are difficulties associated with many applications because CID does not provide sufficient information to permit details of the molecular structures to be elucidated, including post-translational-modifications in proteomics, as well as isomer differentiation in metabolomics and lipidomics. To face these challenges, we are developing fast electron-based dissociation devices using a novel radio-frequency ion trap (i...
2017: Mass Spectrometry
https://www.readbyqxmd.com/read/28630030/alzheimer-s-brains-show-inter-related-changes-in-rna-and-lipid-metabolism
#2
Shahar Barbash, Benjamin P Garfinkel, Rotem Maoz, Alon Simchovitz, Bettina Nadorp, Alessandro Guffanti, Estelle R Bennett, Courtney Nadeau, Andreas Türk, Lukas Paul, Torsten Reda, Yan Li, Aron S Buchman, David S Greenberg, Alexander Seitz, David A Bennett, Patrick Giavalisco, Hermona Soreq
Alzheimer's disease (AD) involves changes in both lipid and RNA metabolism, but it remained unknown if these differences associate with AD's cognition and/or post-mortem neuropathology indices. Here, we report RNA-sequencing evidence of inter-related associations between lipid processing, cognition level, and AD neuropathology. In two unrelated cohorts, we identified pathway-enriched facilitation of lipid processing and alternative splicing genes, including the neuronal-enriched NOVA1 and hnRNPA1. Specifically, this association emerged in temporal lobe tissue samples from donors where postmortem evidence demonstrated AD neuropathology, but who presented normal cognition proximate to death...
June 16, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28624795/tissue-and-serum-lipidome-shows-altered-lipid-composition-with-diagnostic-potential-in-mycosis-fungoides
#3
Chenchen Xu, Dan Zhou, Yixin Luo, Shuai Guo, Tao Wang, Jie Liu, Yuehua Liu, Zhili Li
Mycosis fungoides (MF) is the most common type of cutaneous T cell lymphoma. In this study, we used matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR-MS) to perform lipidomic profiling of 5 MF tissue samples and 44 serum samples (22 from MF patients and 22 from control subjects). Multivariate statistical analysis of the mass spectral data showed that MF tissues had altered levels of seven lipids and MF sera had altered levels of twelve. Among these, six phosphotidylcholines, PC (34:2), PC (34:1), PC (36:3), PC (36:2), PC (32:0), and PC (38:4) and one sphingomyelin, SM (16:0) were altered in both MF tissues and sera...
May 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28623287/heritability-and-responses-to-high-fat-diet-of-plasma-lipidomics-in-a-twin-study
#4
Turid Frahnow, Martin A Osterhoff, Silke Hornemann, Michael Kruse, Michal A Surma, Christian Klose, Kai Simons, Andreas F H Pfeiffer
Lipidomics have a great potential as clinical tool for monitoring metabolic changes in health and disease. Nevertheless hardly anything is known about the heritability of lipids. Therefore, it is necessary to clarify how and how much we can affect these progresses in individuals. In our interventional twin study (46 healthy, non-obese twin pairs) we investigated the lipid profile in plasma samples after switching from a low fat diet to an isocaloric high fat diet (HFD) to characterize the metabolic adaptation...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28618037/hydrogen-peroxide-stimulates-exosomal-cathepsin-b-regulation-of-the-receptor-for-advanced-glycation-end-products-rage
#5
Charles A Downs, Viet D Dang, Nicholle M Johnson, Nancy Denslow, Abdel A Alli
Exosomes are nano-sized vesicles that are secreted into the extracellular environment. These vesicles contain various biological effector molecules that can regulate intracellular signaling pathways in recipient cells. The aim of this study was to examine a correlation between exosomal cathepsin B activity and the receptor for advanced glycation end-products (RAGE). Type 1 alveolar epithelial (R3/1) cells were treated with or without hydrogen peroxide and exosomes isolated from the cell conditioned media were characterized by NanoSight analysis...
June 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28607507/urinary-lipidomics-evidence-for-multiple-sources-and-sexual-dimorphism-in-healthy-individuals
#6
J Graessler, C S Mehnert, K-M Schulte, S Bergmann, S Strauss, T D Bornstein, J Licinio, M-L Wong, A L Birkenfeld, S R Bornstein
Urinary lipidomics may add new valuable biomarkers to the diagnostic armamentarium for early detection of metabolic and kidney diseases. Sources and composition of urinary lipids in healthy individuals, however, have not been investigated in detail. Shotgun lipidomics was used to quantify lipidomic profiles in native urine samples from 16 individuals (eight men, eight women) collected in five fractions over 24 h. All probands were comprehensively characterized by urinary and clinical indices. The mean total urinary lipid concentration per sample was 0...
June 13, 2017: Pharmacogenomics Journal
https://www.readbyqxmd.com/read/28607433/lipidomic-analysis-of-skeletal-muscle-tissues-of-p53-knockout-mice-by-nuplc-esi-ms-ms
#7
Se Mi Park, Seul Kee Byeon, Hojun Lee, Hyerim Sung, Il Yong Kim, Je Kyung Seong, Myeong Hee Moon
Tumour suppressor p53 is known to be associated with the maintenance of mitochondrial functional properties in the skeletal muscles. As deactivation or mutation of p53 can affect the synthesis of lipids, investigating the relationship between p53-related energy generation metabolism and perturbation of lipid profile is critical. In this study, 329 lipid species (among 412 identified species) in two different skeletal muscle tissues (the gastrocnemius and soleus) from p53 knockout (KO) mice were quantitatively analysed using nanoflow ultrahigh performance liquid chromatography tandem mass spectrometry (nUPLC-MS/MS)...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28607345/coral-lipid-bodies-as-the-relay-center-interconnecting-diel-dependent-lipidomic-changes-in-different-cellular-compartments
#8
Hung-Kai Chen, Li-Hsueh Wang, Wan-Nan U Chen, Anderson B Mayfield, Oren Levy, Chan-Shing Lin, Chii-Shiarng Chen
Lipid bodies (LBs) in the coral gastrodermal tissues are key organelles in the regulation of endosymbiosis and exhibit a diel rhythmicity. Using the scleractinian Euphyllia glabrescens collected across the diel cycle, we observed temporally dynamic lipid profiles in three cellular compartments: host coral gastrodermal cells, LBs, and in hospite Symbiodinium. Particularly, the lipidome varied over time, demonstrating the temporally variable nature of the coral-Symbiodinium endosymbiosis. The lipidome-scale data highlight the dynamic, light-driven metabolism of such associations and reveal that LBs are not only lipid storage organelles but also act as a relay center in metabolic trafficking...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28605664/soy-compared-with-milk-protein-in-a-western-diet-changes-fecal-microbiota-and-decreases-hepatic-steatosis-in-obese-oletf-rats
#9
Matthew R Panasevich, Colin M Schuster, Kathryn E Phillips, Grace M Meers, Sree V Chintapalli, Umesh D Wankhade, Kartik Shankar, Dustie N Butteiger, Elaine S Krul, John P Thyfault, R Scott Rector
Soy protein is effective at preventing hepatic steatosis; however, the mechanisms are poorly understood. We tested the hypothesis that soy vs. dairy protein-based diet would alter microbiota and attenuate hepatic steatosis in hyperphagic Otsuka Long-Evans Tokushima fatty (OLETF) rats. Male OLETF rats were randomized to "Western" diets containing milk protein isolate (MPI), soy protein isolate (SPI) or 50:50 MPI/SPI (MS) (n=9-10/group; 21% kcal protein) for 16 weeks. SPI attenuated (P<.05) fat mass and percent fat by ~10% compared with MS, but not compared with MPI...
May 31, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28605387/a-simple-fractionated-extraction-method-for-the-comprehensive-analysis-of-metabolites-lipids-and-proteins-from-a-single-sample
#10
Mohamed Salem, Michal Bernach, Krzysztof Bajdzienko, Patrick Giavalisco
Understanding of complex biological systems requires the measurement, analysis and integration of multiple compound classes of the living cell, usually determined by transcriptomic, proteomic, metabolomics and lipidomic measurements. In this protocol, we introduce a simple method for the reproducible extraction of metabolites, lipids and proteins from biological tissues using a single aliquot per sample. The extraction method is based on a methyl tert-butyl ether: methanol: water system for liquid: liquid partitioning of hydrophobic and polar metabolites into two immiscible phases along with the precipitation of proteins and other macromolecules as a solid pellet...
June 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28598845/discovery-and-confirmation-of-diagnostic-serum-lipid-biomarkers-for-alzheimer-s-disease-using-direct-infusion-mass-spectrometry
#11
Swati Anand, Justin M Barnes, Sydney A Young, Diana M Garcia, H Dennis Tolley, John S K Kauwe, Steven W Graves
Alzheimer's disease (AD) is a neurodegenerative disorder lacking early biochemical diagnosis and treatment. Lipids have been implicated in neurodegenerative disorders including AD. A shotgun lipidomic approach was undertaken to determine if lipid biomarkers exist that can discriminate AD cases from controls. The discovery study involved sera from 29 different stage AD cases and 32 controls. Lipid extraction was performed using organic solvent and the samples were directly infused into a time-of-flight mass spectrometer...
June 9, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28598843/a-lipidomics-approach-to-assess-the%C3%A2-association-between-plasma-sphingolipids-and-verbal-memory-performance-in-coronary-artery-disease-patients-undertaking-cardiac-rehabilitation-a-c18-0-signature-for%C3%A2-cognitive-response-to-exercise
#12
Mahwesh Saleem, Nathan Herrmann, Adam Dinoff, Michelle M Mielke, Paul I Oh, Prathiba Shammi, Xingshan Cao, Swarajya Lakshmi Vattem Venkata, Norman J Haughey, Krista L Lanctôt
BACKGROUND: Early subtle deficits in verbal memory, which may indicate early neural risk, are common in patients with coronary artery disease (CAD). While exercise can improve cognition, cognitive response to exercise is heterogeneous. Sphingolipids have been associated with the development and progression of CAD, and impairments in sphingolipid metabolism may play roles in neurodegeneration and in the neural adaptation response to exercise. OBJECTIVE: In this study, change in plasma concentrations of sphingolipids was assessed in relation to change in verbal memory performance and in other cognitive domains among CAD subjects undertaking a 6-month cardiac rehabilitation (CR) program...
June 3, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28596719/a-methodology-for-elucidating-regulatory-mechanisms-leading-to-changes-in-lipid-profiles
#13
Ferran Casbas Pinto, Srinivarao Ravipati, David A Barrett, T Charles Hodgman
INTRODUCTION: It is difficult to elucidate the metabolic and regulatory factors causing lipidome perturbations. OBJECTIVES: This work simplifies this process. METHODS: A method has been developed to query an online holistic lipid metabolic network (of 7923 metabolites) to extract the pathways that connect the input list of lipids. RESULTS: The output enables pathway visualisation and the querying of other databases to identify potential regulators...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28590551/ozone-induced-dissociation-on-a-traveling-wave-high-resolution-mass-spectrometer-for-determination-of-double-bond-position-in-lipids
#14
Ngoc Vu, Jeffery Brown, Kevin Giles, Qibin Zhang
RATIONALE: The position of C=C within fatty acyl chains affects the biological function of lipids. Ozone induced dissociation-mass spectrometry (OzID-MS) has great potential in determination of lipid double bond position, but has only been implemented on low resolution ion trap mass spectrometers. In addition, most of the OzID-MS experiments carried out so far were focused on the sodiated adducts of lipids, fragmentation of the most commonly observed protonated ions generated in LC-MS based lipidomics workflow has been less explored...
June 7, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28587463/lipidomic-profiling-of-human-adipose-tissue-identifies-a-pattern-of-lipids-associated-with-fish-oil-supplementation
#15
Elizabeth G Stanley, Benjamin J Jenkins, Celia G Walker, Albert Koulman, Annette L West, Philip C Calder, Susan A Jebb, Julian Leether Griffin
To understand the interaction between diet and health, biomarkers that accurately reflect consumption of foods of perceived health relevance are needed. The aim of this investigation was to use direct infusion-mass spectrometry (DI-MS) lipidomics to determine the effects of fish oil supplementation on lipid profiles of human adipose tissue. Adipose tissue samples from an n-3 polyunsaturated fatty acid (PUFA) supplementation study (n = 66) were analysed to compare the pattern following supplementation equivalent to 0 or 4 portions of oily fish per week...
June 6, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28586382/vibrio-cholerae-ensures-function-of-host-proteins-required-for-virulence-through-consumption-of-luminal-methionine-sulfoxide
#16
Audrey S Vanhove, Saiyu Hang, Vidhya Vijayakumar, Adam Cn Wong, John M Asara, Paula I Watnick
Vibrio cholerae is a diarrheal pathogen that induces accumulation of lipid droplets in enterocytes, leading to lethal infection of the model host Drosophila melanogaster. Through untargeted lipidomics, we provide evidence that this process is the product of a host phospholipid degradation cascade that induces lipid droplet coalescence in enterocytes. This infection-induced cascade is inhibited by mutation of the V. cholerae glycine cleavage system due to intestinal accumulation of methionine sulfoxide (MetO), and both dietary supplementation with MetO and enterocyte knock-down of host methionine sulfoxide reductase A (MsrA) yield increased resistance to infection...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28582856/lipidomic-profiles-in-diabetes-and%C3%A2-dementia
#17
Kevin Huynh, Ralph N Martins, Peter J Meikle
Lipids are a diverse class of hydrophobic and amphiphilic molecules which make up the bulk of most biological systems and are essential for human life. The role of lipids in health and disease has been recognized for many decades, as evidenced by the early identification of cholesterol as an important risk factor of heart disease and the development and introduction of statins as a one of the most successful therapeutic interventions to date. While several studies have demonstrated an increased risk of dementia, including Alzheimer's disease (AD), in those with diabetes mellitus, the nature of this risk is not well understood...
May 30, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28570056/intensity-independent-noise-filtering-in-ft-ms-and-ft-ms-ms-spectra-for-shotgun-lipidomics
#18
Kai Schuhmann, Henrik Thomas, Jacobo Miranda Ackerman, Konstantin O Nagornov, Yury O Tsybin, Andrej Shevchenko
Shotgun lipidomics relies on the direct infusion of total lipid extracts into a high resolution tandem mass spectrometer. A single shotgun analysis produces several hundred of densely populated FT MS and FT MS/MS spectra, each of which might comprise thousands of peaks although a very small percentage of those belong to lipids. Eliminating noise by adjusting a minimal peak intensity threshold is biased and inefficient since lipid species and classes vary in their natural abundance and ionization capacity. We developed a method of peak intensity-independent noise filtering in shotgun FT MS and FT MS/MS spectra that capitalizes on a stable composition of the infused analyte leading to consistent time-independent detection of its bona fide components...
June 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28564599/er-stress-inhibits-liver-fatty-acid-oxidation-while-unmitigated-stress-leads-to-anorexia-induced-lipolysis-and-both-liver-and-kidney-steatosis
#19
Diane DeZwaan-McCabe, Ryan D Sheldon, Michelle C Gorecki, Deng-Fu Guo, Erica R Gansemer, Randal J Kaufman, Kamal Rahmouni, Matthew P Gillum, Eric B Taylor, Lynn M Teesch, D Thomas Rutkowski
The unfolded protein response (UPR), induced by endoplasmic reticulum (ER) stress, regulates the expression of factors that restore protein folding homeostasis. However, in the liver and kidney, ER stress also leads to lipid accumulation, accompanied at least in the liver by transcriptional suppression of metabolic genes. The mechanisms of this accumulation, including which pathways contribute to the phenotype in each organ, are unclear. We combined gene expression profiling, biochemical assays, and untargeted lipidomics to understand the basis of stress-dependent lipid accumulation, taking advantage of enhanced hepatic and renal steatosis in mice lacking the ER stress sensor ATF6α...
May 30, 2017: Cell Reports
https://www.readbyqxmd.com/read/28556292/a-review-of-the-role-of-sebum-in-the-mechanism-of-acne-pathogenesis
#20
REVIEW
Xinchao Li, Congfen He, Zhou Chen, Cheng Zhou, Yao Gan, Yan Jia
Acne is one of the most common skin disorders, and its occurrence is closely related to many factors, including sebum secretion, hormone levels, bacterial infection, and inflammatory reactions. Among these, changes in sebum secretion are believed to be one important factor of acne. Increased sebum secretion can induce acne occurrence, and increasing evidence indicates sebum component changes are also strongly related to acne occurrence. Recently, developments in lipidomics have provided effective lipid analysis methods...
May 29, 2017: Journal of Cosmetic Dermatology
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