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https://www.readbyqxmd.com/read/28340309/analytical-techniques-in-lipidomics-state-of-the-art
#1
Kamil Jurowski, Kamila Kochan, Justyna Walczak, Małgorzata Barańska, Wojciech Piekoszewski, Bogusław Buszewski
Current studies related to lipid identification and determination, or lipidomics in biological samples, are one of the most important issues in modern bioanalytical chemistry. There are many articles dedicated to specific analytical strategies used in lipidomics in various kinds of biological samples. However, in such literature, there is lack of articles dedicated to a comprehensive review of the actual analytical methodologies used in lipidomics. The aim of this article is to characterize the lipidomics methods used in modern bioanalysis according to the methodological point of view: 1) chromatography/separation methods, 2) spectroscopic methods and 3) mass spectrometry and also hyphenated methods...
March 24, 2017: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/28340140/lipidomic-profiling-of-endometrial-fluid-in-women-with-ovarian-endometriosis
#2
Francisco Domínguez, Marcos Ferrando, Patricia Díaz-Gimeno, Fernando Quintana, Gemma Fernández, Inés Castells, Carlos Simón
The proteomic content of the endometrial fluid from patients with endometriosis has been investigated, but the lipidomic profile has not been analyzed yet in detail. This study is a comparative untargeted lipidomic analysis of human endometrial fluid obtained from thirty-five patients (12 endometriosis and 23 controls). Global differential lipidomic profile was analyzed in both groups by ultrahigh performance liquid chromatography (UPLC) coupled to mass spectrometry (MS). 123 out of the 457 metabolites identified in the endometrial fluid were found to be significantly differentially expressed between ovarian endometriosis versus controls...
March 18, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28339984/the-link-between-phenotype-and-fatty-acid-metabolism-in-advanced-chronic-kidney-disease
#3
Dan-Qian Chen, Hua Chen, Lin Chen, Nosratola D Vaziri, Ming Wang, Xiang-Ri Li, Ying-Yong Zhao
Background.: The kidney plays a central role in elimination of metabolic waste products and regulation of low-molecular weight metabolites via glomerular filtration, tubular secretion and reabsorption. Disruption of these processes results in profound changes in the biochemical milieu of the body fluids, which contribute to complications of chronic kidney disease (CKD) by inducing cytotoxicity and inflammation. Insight into the changes of the composition of metabolites and dysregulation of target genes and proteins enhances the understanding of the pathophysiology of CKD and its complications, and the development of novel therapeutic strategies...
March 1, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28333036/apoe-%C3%AE%C2%B54-specific-imbalance-of-arachidonic-acid-and-docosahexaenoic-acid-in-serum-phospholipids-identifies-individuals-with-preclinical-mild-cognitive-impairment-alzheimer-s-disease
#4
Laila Abdullah, James E Evans, Tanja Emmerich, Gogce Crynen, Ben Shackleton, Andrew P Keegan, Cheryl Luis, Leon Tai, Mary J LaDu, Michael Mullan, Fiona Crawford, Corbin Bachmeier
This study was designed to explore the influence of apolipoprotein E (APOE) on blood phospholipids (PL) in predicting preclinical Alzheimer's disease (AD). Lipidomic analyses were also performed on blood from an AD mouse model expressing human APOE isoforms (EFAD) and five AD mutations and from 195 cognitively normal participants, 23 of who converted to mild cognitive impairment (MCI)/AD within 3 years. APOE ε4-carriers converting to MCI/AD had high arachidonic acid (AA)/docosahexaenoic acid (DHA) ratios in PL compared to cognitively normal ε4 and non-ε4 carriers...
March 23, 2017: Aging
https://www.readbyqxmd.com/read/28331812/the-plasma-lipidome-in-acute-myeloid-leukemia-at-diagnosis-in-relation-to-clinical-disease-features
#5
Thomas Pabst, Linda Kortz, Georg M Fiedler, Uta Ceglarek, Jeffrey R Idle, Diren Beyoğlu
BACKGROUND: Early studies established that certain lipids were lower in acute myeloid leukemia (AML) cells than normal leukocytes. Because lipids are now known to play an important role in cell signaling and regulation of homeostasis, and are often perturbed in malignancies, we undertook a comprehensive lipidomic survey of plasma from AML patients at time of diagnosis and also healthy blood donors. METHODS: Plasma lipid profiles were measured using three mass spectrometry platforms in 20 AML patients and 20 healthy blood donors...
June 2017: BBA Clinical
https://www.readbyqxmd.com/read/28327659/the-immunosuppressant-mycophenolic-acid-alters-nucleotide-and-lipid-metabolism-in-an-intestinal-cell-model
#6
Svenja Heischmann, Monika Dzieciatkowska, Kirk Hansen, Dieter Leibfritz, Uwe Christians
The study objective was to elucidate the molecular mechanisms underlying the negative effects of mycophenolic acid (MPA) on human intestinal cells. Effects of MPA exposure and guanosine supplementation on nucleotide concentrations in LS180 cells were assessed using liquid chromatography-mass spectrometry. Proteomics analysis was carried out using stable isotope labeling by amino acids in cell culture combined with gel-based liquid chromatography-mass spectrometry and lipidome analysis using (1)H nuclear magnetic resonance spectroscopy...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28325886/mouse-mitochondrial-lipid-composition-is-defined-by-age-in-brain-and-muscle
#7
Amelia K Pollard, Catharine A Ortori, Reinhard Stöger, David A Barrett, Lisa Chakrabarti
Functionality of the lipid rich mitochondrial organelle declines with increased age. Recent advances in lipidomic technologies allowed us to perform a global characterisation of lipid composition in two different tissue types and age ranges. Ultra-high performance liquid chromatography coupled with high resolution mass spectrometry was used to establish and compare mitochondrial lipidomes of brain and skeletal muscle from young (4-11 weeks old) and middle age (78 weeks old) healthy mice. In middle age the brain mitochondria had reduced levels of fatty acids, particularly polyunsaturated fatty acids, while skeletal muscle mitochondria had a decreased abundance of phosphatidylethanolamine, but a pronounced increase of triglyceride levels...
March 21, 2017: Aging
https://www.readbyqxmd.com/read/28323975/muscle-sympathetic-nerve-activity-is-associated-with-elements-of-the-plasma-lipidomic-profile-in-young-asian-adults
#8
Nina Eikelis, Elisabeth A Lambert, Sarah Phillips, Carolina Ika Sari, Piyushkumar A Mundra, Jacquelyn M Weir, Kevin Huynh, Mariee T Grima, Nora E Straznicky, John B Dixon, Markus P Schlaich, Peter J Meikle, Gavin W Lambert
Background: Asian subjects are at increased cardio-metabolic risk at comparatively lower BMI compared with Caucasians. Sympathetic nervous system activation and dyslipidaemia, both characteristics of increased adiposity, appear to be related. We therefore analysed the association of muscle sympathetic nerve activity (MSNA) with the plasma lipidomic profile in young Asian and Caucasian subjects. Methods: Blood samples were collected from 101 participants of either Asian or Caucasian background, aged 18-30, BMI 28...
March 15, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28323950/breaking-up-prolonged-sitting-alters-the-postprandial-plasma-lipidomic-profile-of-adults-with-type-2-diabetes
#9
Megan S Grace, Paddy C Dempsey, Parneet Sethi, Piyushkumar A Mundra, Natalie A Mellett, Jacquelyn M Weir, Neville Owen, David W Dunstan, Peter J Meikle, Bronwyn A Kingwell
Context: Postprandial dysmetabolism in type 2 diabetes (T2D) is exacerbated by prolonged sitting, and may trigger inflammation and oxidative stress. It is unknown what impact countermeasures to prolonged sitting have on the postprandial lipidome. Objective: In this study, we investigated the effects of regular interruptions to sitting, compared to prolonged sitting, on the postprandial plasma lipidome. Design: Randomized cross-over experimental trial...
March 13, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28319722/bisphenol-s-exposure-modulate-macrophage-phenotype-as-defined-by-cytokines-profiling-global-metabolomics-and-lipidomics-analysis
#10
Chao Zhao, Zhi Tang, Jiacheng Yan, Jing Fang, Hailin Wang, Zongwei Cai
As an important structural analogue of bisphenol A (BPA), bisphenol S (BPS) has been used as alternatives to BPA in industrialized production. However, the immunotoxicity of BPS remains poorly understood. As a critical model in inflammatory responses, macrophages are used to explore the immunotoxic potential and mechanisms of BPS at environmentally relevant concentrations in our study. Here, we are combining molecular toxicology and mass spectrometry (MS)-based global metabolomics and lipidomics study together to estimate the variation of cytokines profiling and metabolism characteristic following BPS exposure...
March 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28317846/serum-and-synovial-fluid-lipidomic-profiles-predict-obesity-associated-osteoarthritis-synovitis-and-wound-repair
#11
Chia-Lung Wu, Kelly A Kimmerling, Dianne Little, Farshid Guilak
High-fat diet-induced obesity is a major risk factor for osteoarthritis (OA) and diminished wound healing. The objective of this study was to determine the associations among serum and synovial fluid lipid levels with OA, synovitis, adipokine levels, and wound healing in a pre-clinical obese mouse model of OA. Male C57BL/6 J mice were fed either a low-fat (10% kcal) or one of three high-fat (HF, 60% kcal) diets rich in saturated fatty acids (SFAs), ω-6 or ω-3 polyunsaturated FAs (PUFAs). OA was induced by destabilization of the medial meniscus...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28316657/comprehensive-identification-of-sphingolipid-species-by-in-silico-retention-time-and-tandem-mass-spectral-library
#12
Hiroshi Tsugawa, Kazutaka Ikeda, Wataru Tanaka, Yuya Senoo, Makoto Arita, Masanori Arita
BACKGROUND: Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is used for comprehensive metabolome and lipidome analyses. Compound identification relies on similarity matching of the retention time (RT), precursor m/z, isotopic ratio, and MS/MS spectrum with reference compounds. For sphingolipids, however, little information on the RT and MS/MS references is available. RESULTS: Negative-ion ESI-MS/MS is a useful method for the structural characterization of sphingolipids...
2017: Journal of Cheminformatics
https://www.readbyqxmd.com/read/28314772/two-phylogenetically-and-compartmentally-distinct-cdp-diacylglycerol-synthases-cooperate-for-lipid-biogenesis-in-toxoplasma-gondii
#13
Pengfei Kong, Christoph-Martin Ufermann, Diana Lm Zimmermann, Qing Yin, Xun Suo, J Bernd Helms, Jos F Brouwers, Nishith Gupta
Toxoplasma gondii is among the most prevalent protozoan parasites, which infects a wide range of organisms including one-third of the human population. Its rapid intracellular replication within a vacuole requires efficient synthesis of glycerophospholipids. Cytidine diphosphate-diacylglycerol (CDP-DAG) serves as a major precursor for phospholipid synthesis. Given the peculiarities of lipid biogenesis, understanding the mechanism and physiological importance of CDP-DAG synthesis is particularly relevant in T...
March 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28302590/stable-isotope-analysis-of-dynamic-lipidomics
#14
Joost Brandsma, Andrew P Bailey, Grielof Koster, Alex P Gould, Anthony D Postle
Metabolic pathway flux is a fundamental element of biological activity, which can be quantified using a variety of mass spectrometric techniques to monitor incorporation of stable isotope-labelled substrates into metabolic products. This article contrasts developments in electrospray ionisation mass spectrometry (ESI-MS) for the measurement of lipid metabolism with more established gas chromatography mass spectrometry and isotope ratio mass spectrometry methodologies. ESI-MS combined with diagnostic tandem MS/MS scans permits the sensitive and specific analysis of stable isotope-labelled substrates into intact lipid molecular species without the requirement for lipid hydrolysis and derivatisation...
March 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28301461/metabolomics-and-lipidomics-using-traveling-wave-ion-mobility-mass-spectrometry
#15
Giuseppe Paglia, Giuseppe Astarita
Metabolomics and lipidomics aim to profile the wide range of metabolites and lipids that are present in biological samples. Recently, ion mobility spectrometry (IMS) has been used to support metabolomics and lipidomics applications to facilitate the separation and the identification of complex mixtures of analytes. IMS is a gas-phase electrophoretic technique that enables the separation of ions in the gas phase according to their charge, shape and size. Occurring within milliseconds, IMS separation is compatible with modern mass spectrometry (MS) operating with microsecond scan speeds...
April 2017: Nature Protocols
https://www.readbyqxmd.com/read/28297799/-a-serum-lipidomic-study-of-patients-with-non-alcoholic-fatty-liver-disease
#16
R X Yang, C X Hu, Y Q Mi, W L Sun, G Y Chen, Q Pan, F Shen, G W Xu, J G Fan
Objective: To investigate the serum lipidomic profile in patients with nonalcoholic fatty liver disease (NAFLD), and to analyze the lipid metabolism characteristics of NAFLD. Methods: The subjects were divided into control group (23 patients) and pathologically confirmed NAFLD group (42 patients), and ultra-high-performance liquid chromatography-tandem mass spectrometry was used to measure serum lipidomic metabolites. The partial least squares-discriminant analysis (PLS-DA) model was established to analyze the differences in lipid metabolism with reference to the univariate analysis...
February 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/28294984/defining-the-baseline-and-oxidant-perturbed-lipidomic-profiles-of-daphnia-magna
#17
Nadine S Taylor, Thomas A White, Mark R Viant
Recent technological advancement has enabled the emergence of lipidomics as an important tool for assessing molecular stress, one which has yet to be assessed fully as an approach in an environmental toxicological context. Here we have applied a high-resolution, non-targeted, nanoelectrospray ionisation (nESI) direct infusion mass spectrometry (DIMS) technique to assess the effects of oxidative stress to Daphnia magna both in vitro (air exposure of daphniid extracts) and in vivo (Cu(2+) exposure). Multivariate and univariate statistical analyses were used to distinguish any perturbations including oxidation to the D...
March 15, 2017: Metabolites
https://www.readbyqxmd.com/read/28294227/raman-spectroscopy-compatible-pdms-droplet-microfluidic-culture-and-analysis-platform-towards-on-chip-lipidomics
#18
Hyun Soo Kim, Sergio C Waqued, Dawson T Nodurft, Timothy P Devarenne, Vladislav V Yakovlev, Arum Han
Lipids produced by microalgae are viewed as a potential renewable alternative to fossil fuels, however, significant improvements in productivity are required for microalgal biofuels to become economically feasible. Here we present a method that allows for the use of Raman spectroscopy with poly(dimethylsiloxane) (PDMS) droplet microfluidic devices, which not only overcomes the high Raman background of PDMS, but also achieves pairing of the high-throughput single-cell resolution advantages of droplet microfluidics with the direct, chemically specific, label-free, and non-destructive nature of Raman spectroscopy...
March 15, 2017: Analyst
https://www.readbyqxmd.com/read/28291223/liver-specific-deletion-of-the-plpp3-gene-alters-plasma-lipid-composition-and-worsens-atherosclerosis-in-apoe-mice
#19
Marco Busnelli, Stefano Manzini, Mika Hilvo, Cinzia Parolini, Giulia S Ganzetti, Federica Dellera, Kim Ekroos, Minna Jänis, Diana Escalante-Alcalde, Cesare R Sirtori, Reijo Laaksonen, Giulia Chiesa
The PLPP3 gene encodes for a ubiquitous enzyme that dephosphorylates several lipid substrates. Genome-wide association studies identified PLPP3 as a gene that plays a role in coronary artery disease susceptibility. The aim of the study was to investigate the effect of Plpp3 deletion on atherosclerosis development in mice. Because the constitutive deletion of Plpp3 in mice is lethal, conditional Plpp3 hepatocyte-specific null mice were generated by crossing floxed Plpp3 mice with animals expressing Cre recombinase under control of the albumin promoter...
March 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28287421/in-depth-lipidomic-analysis-of-molecular-species-of-triacylglycerides-diacylglycerides-glycerophospholipids-and-sphingolipids-of-buttermilk-by-gc-ms-fid-hplc-elsd-and-uplc-qtof-ms
#20
Pilar Castro-Gómez, Olimpio Montero, Javier Fontecha
Buttermilk, a byproduct of butter manufacturing, has gained considerable attention due to its high concentration of polar lipids as phospho- and sphingolipids from the milk fat globule membrane (MFGM). These polar lipids (PLs) are essential components of all cellular membranes and exert a variety of indispensable metabolic, neurological, and intracellular signaling processes. Despite its importance, there are few research studies that report a comprehensive characterization of the lipid molecular species of MFGM that could contribute to a better understanding of their putative healthful activities...
March 10, 2017: International Journal of Molecular Sciences
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