keyword
MENU ▼
Read by QxMD icon Read
search

pilocarpine

keyword
https://www.readbyqxmd.com/read/28432779/nmdar-independent-hippocampal-long-term-depression-impairment-after-status-epilepticus-in-a-lithium-pilocarpine-model-of-temporal-lobe-epilepsy
#1
Andrey D Ivanov, Aleksey V Zaitsev
Temporal lobe epilepsy is usually associated with cognitive decline and memory deficits. Despite numerous existing studies on various animal models, the mechanisms of these deficits remain largely unclear. A specific form of long-term synaptic efficacy changes - long-term depression (LTD) - is thought to play an important role in memory formation and learning. However, extremely little is known about the possible alteration of LTD induction and dynamics after a status epilepticus (SE). In the present work we investigated the acute and delayed effects of lithium-pilocarpine-induced SE on NMDAR-dependent and NMDAR-independent hippocampal LTD in vitro...
April 22, 2017: Synapse
https://www.readbyqxmd.com/read/28431266/reduced-abnormal-integration-of-adult-generated-granule-cells-does-not-attenuate-spontaneous-recurrent-seizures-in-mice
#2
Kun Zhu, Bo Yuan, Ming Hu, Gai-Feng Feng, Yong Liu, Jian-Xin Liu
Epileptic seizures lead to aberrant hippocampal neurogenesis, including increased proliferation of neural progenitors and abnormal integrations of newly generated granule cells - hilar ectopic granule cells (EGCs), mossy fiber sprouting (MFS), and hilar basal dendrites (HBDs). Previous results from ablating hippocampal neurogenesis after acute seizures have been controversial with regards to the development of spontaneous recurrent seizures (SRSs). While ablation of hippocampal newborn cells was effective, a sufficient decrease of subsequent abnormal integrations in chronically epileptic hippocampus was not well-established in these studies...
April 11, 2017: Epilepsy Research
https://www.readbyqxmd.com/read/28429515/a-device-to-measure-secretion-of-individual-sweat-glands-for-diagnosis-of-peripheral-neuropathy
#3
Adam J Loavenbruck, James S Hodges, Vincenzo Provitera, Maria Nolano, Gwen Wendelshafer-Crabb, William R Kennedy
INTRODUCTION: There is a need for quantitative, precise assessment of small fiber peripheral nerve function. We tested a customized camera device and protocol designed to quantify secretions of individual sweat glands (SGs). MATERIALS AND METHODS: Testing was performed on 178 healthy controls and 20 neuropathy subjects. Sweating was stimulated on a 2.25 cm(2) skin area by iontophoresis of pilocarpine. The camera imaged sweat from 50-400 sweat ducts. We calculated secretion rate of individual SGs, total sweat volume, and number of secreting SGs at four body sites...
April 21, 2017: Journal of the Peripheral Nervous System: JPNS
https://www.readbyqxmd.com/read/28429259/early-morphological-and-functional-changes-in-the-gabaergic-system-of-hippocampus-in-the-rat-lithium-pilocarpine-model-of-epilepsy
#4
V B Karyakin, D S Vasil'ev, I A Zhuravin, A V Zaitsev, L G Magazanik
We studied early alterations in the GABAergic system of the rat hippocampus in the lithium-pilocarpine model of epilepsy. Twenty-four hours after the pilocarpine treatment, a decrease in the number of calretinin-positive interneurons was observed in the CA1 field of the hippocampus, whereas the number of parvalbumin-positive interneurons remained unchanged. The decreased levels of the GABA-synthesizing enzyme glutamic acid decarboxylase (GAD67) and the membrane GABA transporter GAT1 were revealed using Western blot analysis...
January 2017: Doklady Biological Sciences: Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections
https://www.readbyqxmd.com/read/28427858/mice-with-conditional-neurod1-knockout-display-reduced-aberrant-hippocampal-neurogenesis-but-no-change-in-epileptic-seizures
#5
Rebecca Brulet, Jingfei Zhu, Mahafuza Aktar, Jenny Hsieh, Kyung-Ok Cho
Adult neurogenesis is significantly increased in the hippocampus of rodent models of temporal lobe epilepsy (TLE). These adult-generated neurons have recently been shown to play a contributing role in the development of spontaneous recurrent seizures (SRS). In order to eventually target pro-epileptic adult neurogenesis in the clinical setting, it will be important to identify molecular players involved in the control of aberrant neurogenesis after seizures. Here, we focused on NeuroD1 (ND1), a member of the bHLH family of transcription factors previously shown to play an essential role in the differentiation and maturation of adult-generated neurons in the hippocampus...
April 18, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28425097/seizure-frequency-correlates-with-loss-of-dentate-gyrus-gabaergic-neurons-in-a-mouse-model-of-temporal-lobe-epilepsy
#6
Paul S Buckmaster, Emily Abrams, Xiling Wen
Epilepsy occurs in one of 26 people. Temporal lobe epilepsy is common and can be difficult to treat effectively. It can develop after brain injuries that damage the hippocampus. Multiple pathophysiological mechanisms involving the hippocampal dentate gyrus have been proposed. The present study evaluated a mouse model of temporal lobe epilepsy to test which pathological changes in the dentate gyrus correlate with seizure frequency and help prioritize potential mechanisms for further study. FVB mice (n = 127) that had experienced status epilepticus after systemic treatment with pilocarpine 31 - 61 d earlier were video-monitored for spontaneous, convulsive seizures 9 h/d every day for 24 - 36 d...
April 20, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28416442/allopregnanolone-decreases-interictal-spiking-and-fast-ripples-in-an-animal-model-of-mesial-temporal-lobe-epilepsy
#7
Maxime Lévesque, Rochelle Herrington, Leila Leclerc, Michael A Rogawski, Massimo Avoli
The objective of this study was to characterize the impact of allopregnanolone, a neurosteroid that acts as a positive allosteric modulator of synaptic and extrasynaptic GABAA receptors, on interictal spikes and high-frequency oscillations (ripples: 80-200 Hz, fast ripples: 250-500 Hz) in the pilocarpine model of mesial temporal lobe epilepsy. Seven out of 25 Sprague-Dawley rats experiencing 1 h of pilocarpine-induced status epilepticus (SE) began treatment with allopregnanolone (9.6-12.8 mg/kg/day) on the following day...
April 14, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28415676/nitrobenzylthioinosine-mimics-adenosine-to-attenuate-the-epileptiform-discharge-of-hippocampal-neurons-from-epileptic-rats
#8
Hao Huang, Jing Wang, Jun Zhang, Zhong Luo, Dongxu Li, Xiaowei Qiu, Yan Peng, Zhongxiang Xu, Ping Xu, Zucai Xu
Nitrobenzylthioinosine (NBTI), a specific inhibitor of type 1 equilibrative nucleoside transporter, could regulate the extracellular adenosine concentration and have protective roles in seizures. However, the protection mechanism of NBTI in seizures remains poorly understood. Here, the expression pattern and subcellular distribution of adenosine A1 receptor were detected by Western blot analysis and double-labeling immunofluorescence staining in Lithium Chloride-Pilocarpine induced epileptic rat model. At 24 h after pilocarpine induced rat seizures, hippocampal slices were prepared and the evoked excitatory postsynaptic currents (eEPSCs) amplitude of pyramidal neurons in hippocampus CA1 region was recorded using whole-cell patch clamp...
March 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28410463/vegf-treatment-during-status-epilepticus-attenuates-long-term-seizure-associated-alterations-in-astrocyte-morphology
#9
Janice R Lenzer-Fanara, Thomas Li, Elisa A Salerni, Firmause Payen, Susan D Croll
Vascular endothelial growth factor (VEGF) treatment during pilocarpine-induced status epilepticus (SE) causes sustained preservation of behavioral function in rats in the absence of enduring neuroprotection (Nicoletti et al., 2010), suggesting the possibility that other cells or mechanisms could be involved in the beneficial effects of VEGF during SE. Astrocytes have been reported to contribute to epileptiform discharges in the hippocampus (Tian et al., 2005; Kang et al., 1998) and to express VEGF receptors (Krum & Rosenstein, 2002)...
April 11, 2017: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/28408797/ocular-hypotensive-effects-of-a-rho-associated-protein-kinase-inhibitor-in-rabbits
#10
Muhammad Irfan Kamaruddin, Momoko Nakamura-Shibasaki, Yu Mizuno, Yoshiaki Kiuchi
PURPOSE: Ripasudil is a novel Rho-associated protein kinase inhibitor that is used to treat ocular hypertension. However, the comparison of the intraocular pressure (IOP)-lowering effects between ripasudil alone and other ocular hypotensive drugs has not been studied thoroughly. The purpose of this study is to examine the ocular hypotensive effects of 0.4% ripasudil, 2% pilocarpine, 0.5% timolol and 0.1% dorzolamide in rabbits. We also studied the IOP changes when 0.4% ripasudil was combined with 2% pilocarpine, 0...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28400978/thymoquinone-attenuates-brain-injury-via-an-anti-oxidative-pathway-in-a-status-epilepticus-rat-model
#11
Yi-Ye Shao, Bing Li, Yong-Mei Huang, Qiong Luo, Yang-Mei Xie, Ying-Hui Chen
AIM: Status epilepticus (SE) results in the generation of reactive oxygen species (ROS), which contribute to seizure-induced brain injury. It is well known that oxidative stress plays a pivotal role in status epilepticus (SE). Thymoquinone (TQ) is a bioactive monomer extracted from black cumin (Nigella sativa) seed oil that has anti-inflammatory, anti-cancer, and antioxidant activity in various diseases. This study evaluated the protective effects of TQ on brain injury in a lithium-pilocarpine rat model of SE and investigated the underlying mechanism related to antioxidative pathway...
January 2017: Translational Neuroscience
https://www.readbyqxmd.com/read/28399267/development-of-a-%C3%AE-9-tetrahydrocannabinol-amino-acid-dicarboxylate-prodrug-with-improved-ocular-bioavailability
#12
Goutham R Adelli, Prakash Bhagav, Pranjal Taskar, Tushar Hingorani, Sara Pettaway, Waseem Gul, Mahmoud A ElSohly, Michael A Repka, Soumyajit Majumdar
Purpose: The aim of the present study was to evaluate the utility of the relatively hydrophilic Δ9-tetrahydrocannabinol (THC) prodrugs, mono and di-valine esters (THC-Val and THC-Val-Val) and the amino acid (valine)-dicarboxylic acid (hemisuccinate) ester (THC-Val-HS), with respect to ocular penetration and intraocular pressure (IOP) lowering activity. THC, timolol, and pilocarpine eye drops were used as controls. Methods: THC-Val, THC-Val-Val, and THC-Val-HS were synthesized and chemically characterized...
April 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28396435/intranasal-msc-derived-a1-exosomes-ease-inflammation-and-prevent-abnormal-neurogenesis-and-memory-dysfunction-after-status-epilepticus
#13
Qianfa Long, Dinesh Upadhya, Bharathi Hattiangady, Dong-Ki Kim, Su Yeon An, Bing Shuai, Darwin J Prockop, Ashok K Shetty
Status epilepticus (SE), a medical emergency that is typically terminated through antiepileptic drug treatment, leads to hippocampus dysfunction typified by neurodegeneration, inflammation, altered neurogenesis, as well as cognitive and memory deficits. Here, we examined the effects of intranasal (IN) administration of extracellular vesicles (EVs) secreted from human bone marrow-derived mesenchymal stem cells (MSCs) on SE-induced adverse changes. The EVs used in this study are referred to as A1-exosomes because of their robust antiinflammatory properties...
April 10, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28395287/interleukin-1%C3%AE-plays-a-pivotal-role-via-the-pi3k-akt-mtor-signaling-pathway-in-the-chronicity-of-mesial-temporal-lobe-epilepsy
#14
Zhaohua Xiao, Jing Peng, Na Gan, Ahmed Arafat, Fei Yin
OBJECTIVE: Mesial temporal lobe epilepsy (MTLE) is the most common type of refractory epilepsy. It is often associated with hippocampal sclerosis, which is histopathologically characterized by selective neuron loss, mossy fiber sprouting, and synapse reconstruction, and is the primary cause of refractory epilepsy. Its mechanism has not been fully elucidated. Substantial evidence now supports that inflammatory pathways are activated in epilepsy foci. We have confirmed that the interleukin-1β (IL-1β) level is involved in the epileptogenesis of MTLE, and we further investigated how it works in its chronicity in this study...
April 11, 2017: Neuroimmunomodulation
https://www.readbyqxmd.com/read/28390940/ginkgo-biloba-l-attenuates-spontaneous-recurrent-seizures-and-associated-neurological-conditions-in-lithium-pilocarpine-rat-model-of-temporal-lobe-epilepsy-through-inhibition-of-mammalian-target-of-rapamycin-pathway-hyperactivation
#15
Arindam Ghosh Mazumder, Pallavi Sharma, Vikram Patial, Damanpreet Singh
ETHNOPHARMACOLOGICAL RELEVANCE: Ginkgo biloba L. (Ginkgoaceae) has been widely used in traditional medicine for variety of neurological conditions particularly behavioral and memory impairments. AIM OF THE STUDY: The present study was envisaged to explore the effect of a standardized fraction of Ginkgo biloba leaves (GBbf) in rat model of lithium-pilocarpine induced spontaneous recurrent seizures, and associated behavioral impairments and cognitive deficit. MATERIALS AND METHODS: Rats showing appearance of spontaneous recurrent seizures following lithium pilocarpine (LiPc)-induced status epilepticus (SE) were treated with different doses of GBbf or vehicle for subsequent 4 weeks...
April 5, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28390828/effect-of-gellan-gum-on-the-thermogelation-property-and-drug-release-profile-of-poloxamer-407-based-ophthalmic-formulation
#16
Mitali Dewan, Gunjan Sarkar, Manas Bhowmik, Beauty Das, Atis Kumar Chattoapadhyay, Dipak Rana, Dipankar Chattopadhyay
The effect of gellan gum on the gelation behavior and in-vitro release of a specific drug named pilocarpine hydrochloride from different ophthalmic formulations based on poloxamer 407 is examined. The mixture of 0.3wt% gellan gum and 18wt% poloxamer (PM) solutions show a considerable increase in gel strength in physiological condition. Gel dissolution rate from PM based formulation is significantly decreased due to the addition of gellan gum. FTIR spectra analysis witnesses an interaction in between OH groups of two polymers which accounts for lowering in gelation temperature of PM-gellan gum based formulations...
April 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28387136/the-protective-effect-of-aucubin-from-eucommia-ulmoides-against-status-epilepticus-by-inducing-autophagy-and-inhibiting-necroptosis
#17
Jin Wang, Ying Li, Wei-Hua Huang, Xiang-Chang Zeng, Xiao-Hui Li, Jian Li, Jun Zhou, Jian Xiao, Bo Xiao, Dong-Sheng Ouyang, Kai Hu
Eucommia ulmoides Oliv. is a famous traditional Chinese medicine which exhibits anti-oxidative stress ability and neuro-protective effects. Aucubin is the predominant component of Eucommia ulmoides Oliv. Our present study is intended to investigate aucubin's potential protective effects on neurons against epilepsy in the hippocampus by establishing the lithium-pilocarpine induced status epilepticus (SE) rat model in vivo. Aucubin (at a low dose and a high dose of 5[Formula: see text]mg/kg and 10[Formula: see text]mg/kg, respectively) was administered through gavage for two weeks before lithium-pilocarpine injection...
April 7, 2017: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/28386312/alleviation-of-oxidative-damage-and-involvement-of-nrf2-are-pathway-in-mesodopaminergic-system-and-hippocampus-of-status-epilepticus-rats-pretreated-by-intranasal-pentoxifylline
#18
Yunxiao Kang, Wensheng Yan, Hui Fang, Guoliang Zhang, Yakun Du, Lei Wang, Huixian Cui, Geming Shi
The current studies were aimed at evaluating the efficacy of intranasal pentoxifylline (Ptx) pretreatment in protecting mesodopaminergic system and hippocampus from oxidative damage of lithium-pilocarpine induced status epilepticus (SE) and the involvement of nuclear factor erythroid 2-related factor 2- (Nrf2-) antioxidant response elements pathway. Pentoxifylline was administered to rats intranasally or intraperitoneally 30 minutes before inducing SE. Our results showed the impaired visuospatial memory, the defected mesodopaminergic system, and the oxidative damage and the transient activation of Nrf2 in SE rats...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28378416/tubulin-%C3%AE-iii-modulates-seizure-activity-in-epilepsy
#19
Xin Xu, Yafei Shangguan, Shanshan Lu, Wei Wang, Chao Du, Fei Xiao, Yida Hu, Jing Luo, Liang Wang, Changlong He, Yong Yang, Yanke Zhang, Xi Lu, QinYang, Xuefeng Wang
Beta-III tubulin (TUBB3) is the most dynamic β-tubulin isoform expressed in neurons, and is highly expressed in the central nervous system. However, the relationship between TUBB3 and epileptic seizures has not been thoroughly investigated. This study aimed to investigate the expression of TUBB3 in patients with temporal lobe epilepsy and two different rat models of chronic epilepsy and to determine the specific roles of TUBB3 in epilepsy. TUBB3 expression was upregulated in human and rat epileptic tissue...
April 5, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28372486/the-effect-of-il-1%C3%AE-on-synaptophysin-expression-and-electrophysiology-of-hippocampal-neurons-through-the-pi3k-akt-mtor-signaling-pathway-in-a-rat-model-of-mesial-temporal-lobe-epilepsy
#20
Zhaohua Xiao, Jing Peng, Liwen Wu, Ahmed Arafat, Fei Yin
BACKGROUND: The inflammation induced by interleukin-1β (IL-1β) is a critical factor in the pathogenesis of mesial temporal lobe epilepsy (MTLE). Synaptophysin (SYN) and other changes, including neuron electrophysiology, participate in the pathophysiological processes of MTLE. Phosphatidylinositol 3-kinase (PI3K)/Akt/ mammalian target of rapamycin (mTOR) signaling pathway may play a critical role in regulating SYN expression and electrophysiology of hippocampal neurons. METHODS: We used lithium-pilocarpine-treated rats as model of human MTLE, detecting epileptic seizures with digital video-EEG, and evaluating the proteins related to the PI3K/Akt/mTOR signaling pathway by western blot (WB)...
April 4, 2017: Neurological Research
keyword
keyword
1740
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"