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https://www.readbyqxmd.com/read/29031230/astatine-211-labeled-anti-her2-5f7-single-domain-antibody-fragment-conjugates-radiolabeling-and-preliminary-evaluation
#1
Jaeyeon Choi, Ganesan Vaidyanathan, Eftychia Koumarianou, Choong Mo Kang, Michael R Zalutsky
INTRODUCTION: Derived from heavy chain only camelid antibodies, ~15-kDa single-domain antibody fragments (sdAbs) are an attractive platform for developing molecularly specific imaging probes and targeted radiotherapeutics. The rapid tumor accumulation and normal tissue clearance of sdAbs might be ideal for use with (211)At, a 7.2-h half-life α-emitter, if appropriate labeling chemistry can be devised to trap (211)At in cancer cells after sdAb binding. This study evaluated two reagents, [(211)At]SAGMB and iso-[(211)At]SAGMB, for this purpose...
September 19, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/29027002/camel-molar-tooth-enamel-response-to-gamma-rays-using-epr-spectroscopy
#2
N A El-Faramawy, I El-Somany, A Mansour, A M Maghraby, H Eissa, A Wieser
Tooth enamel samples from molar teeth of camel were prepared using a combined procedure of mechanical and chemical tooth treatment. Based on electron paramagnetic resonance (EPR) spectroscopy, the dose response of tooth enamel samples was examined and compared to that of human enamel. The EPR dose response of the tooth enamel samples was obtained through irradiation to gamma doses from 1 Gy up to 100 kGy. It was found that the radiation-induced EPR signal increased linearly with gamma dose for all studied tooth enamel samples, up to about 15 kGy...
October 12, 2017: Radiation and Environmental Biophysics
https://www.readbyqxmd.com/read/29026532/origins-and-pathogenesis-of-middle-east-respiratory-syndrome-associated-coronavirus-recent-advances
#3
REVIEW
Stephen A Goldstein, Susan R Weiss
Middle East respiratory syndrome-associated coronavirus (MERS-CoV) has been a significant research focus since its discovery in 2012. Since 2012, 2,040 cases and 712 deaths have been recorded (as of August 11, 2017), representing a strikingly high case fatality rate of 36%. Over the last several years, MERS-CoV research has progressed in several parallel and complementary directions. This review will focus on three particular areas: the origins and evolution of MERS-CoV, the challenges and achievements in the development of MERS-CoV animal models, and our understanding of how novel proteins unique to MERS-CoV counter the host immune response...
2017: F1000Research
https://www.readbyqxmd.com/read/29026430/gestational-perinatal-and-postnatal-factors-that-interfere-with-practice-of-exclusive-breastfeeding-by-six-months-after-birth
#4
Mariana Moraes de Oliveira, José Simon Camelo
BACKGROUND: Despite evidences indicating the superiority of breastfeeding and recent advances in the indicators of breastfeeding in Brazil, exclusive breastfeeding (EBF) during the first six months after birth continues to be an infrequent practice in the country. The objective of the present study was to determine which gestational, perinatal, and postnatal factors of the mother-baby dyad might be associated with the cessation of EBF by six months after birth. METHODS: Data were collected at the rooming-in facility of the Reference Center of Women's Health of Ribeirão Preto-Mater (CRSM-Mater) during the postpartum period (24 to 72 h after birth) from December 2012 to April 2013 and by telephone contact between the researcher and participating mothers by six months after birth...
2017: International Breastfeeding Journal
https://www.readbyqxmd.com/read/29026408/camel-milk-has-beneficial-effects-on-diabetes-mellitus-a-systematic-review
#5
REVIEW
Parvin Mirmiran, Hanieh-Sadat Ejtahed, Pooneh Angoorani, Fariba Eslami, Fereidoun Azizi
CONTEXT: Controlling diabetes, a worldwide metabolic disease, by effective alternative treatments is currently a topic of great interest. Camel milk is believed to be a suitable hypoglycemic agent in experimental animals and patients with diabetes. The current systematic review aimed at evaluating the effect of camel milk on diabetes. EVIDENCE ACQUISITION: A comprehensive search was dine in PubMed and Scopus for all clinical trials and animal studies documented up to 2015, which focused on the effect of camel milk on diabetes markers...
April 2017: International Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29024063/influence-of-ration-composition-on-nutritive-and-digestive-variables-in-captive-giraffes-giraffa-camelopardalis-indicating-the-appropriateness-of-feeding-practice
#6
I Gussek, C Große-Brinkhaus, K-H Südekum, J Hummel
The nutrition of captive giraffe (Giraffa camelopardalis), a browsing ruminant, is challenging because browse availability is limited in zoos and rations need to be composed of compensatory feeds. In this study, ration composition for giraffes in 12 German zoos was documented and linked to animal variables that indicate suitability of nutrition. Rations differed in proportion of ration items and chemical composition resulting in various grades of accordance with feeding recommendations. An estimated daily metabolisable energy (ME) intake (MEI; mean ± SD) of 0...
October 10, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29024003/molecular-characterization-of-the-llama-fgf5-gene-and-identification-of-putative-loss-of-function-mutations
#7
M S Daverio, L Vidal-Rioja, E N Frank, F Di Rocco
Llama, the most numerous domestic camelid in Argentina, has good fiber-production ability. Although a few genes related to other productive traits have been characterized, the molecular genetic basis of fiber growth control in camelids is still poorly understood. Fibroblast growth factor 5 (FGF5) is a secreted signaling protein that controls hair growth in humans and other mammals. Mutations in the FGF5 gene have been associated with long-hair phenotypes in several species. Here, we sequenced the llama FGF5 gene, which consists of three exons encoding 813 bp...
October 11, 2017: Animal Genetics
https://www.readbyqxmd.com/read/29022073/the-contribution-of-cell-wall-composition-in-the-expansion-of-camellia-sinensis-seedlings-roots-in-response-to-aluminum
#8
Masoumeh Safari, Faezeh Ghanati, Mohammad Reza Safarnejad, Najmeh Ahmadian Chashmi
Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root. Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood. A plausible relationship between the modifications of the cell wall and the promotion of root elongation was examined in tea seedlings treated for 8 days with 400 µM Al...
October 11, 2017: Planta
https://www.readbyqxmd.com/read/29020952/toxoplasma-gondii-and-neospora-caninum-in-farm-reared-ostriches-struthio-camelus-in-china
#9
Yongjie Feng, Yaoyao Lu, Yinghua Wang, Longxian Zhang, Yurong Yang
BACKGROUND: The parasites Toxoplasma gondii (T. gondii) and Neospora caninum (N. caninum) are globally distributed; they infect warm-blooded animals, including many avian species. The aim of this study was to evaluate the presence of these parasites in ostriches from central China. In total, 402 ostrich (Struthio camelus) samples (293 hearts, 77 brains, and 32 serum) from slaughterhouses of the Henan Province and Hebei Province were collected. The heart juice (n = 283) and serum samples (n = 32) were tested for antibodies to T...
October 11, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28994213/a-novel-and-innovative-hair-test-to-determine-glucocorticoid-levels-in-racing-camels-for-use-in-assessment-of-doping-health-and-disease
#10
Iltaf Shah, Jody D Haddow, Hiba A Ibrahim, Maryam V A Sheikh, Fatima S A Alhemeiri
BACKGROUND: The aim of this project was to develop and validate a new test for the analysis of glucocorticoids in camel hair and to use the new test to analyse hair samples from a variety of camel breeds in sports and racing applications. These findings could be of importance when evaluating racing camels for suspected doping offenses or for injury and disease control. METHODS: Camel hair samples were collected from 30 non-racing dromedary camels along with 3 racing camels in Al Ain, UAE and were decontaminated, pulverized, sonicated and extracted prior to analysis...
October 9, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28993171/cross-sectional-study-of-mers-cov-specific-rna-and-antibodies-in-animals-that-have-had-contact-with-mers-patients-in-saudi-arabia
#11
Samy Kasem, Ibrahim Qasim, Ali Al-Hufofi, Osman Hashim, Ali Alkarar, Ali Abu-Obeida, Albagir Gaafer, Abdelhamid Elfadil, Ahmed Zaki, Ahmed Al-Romaihi, Nasereldeen Babekr, Nadr El-Harby, Raed Hussien, Ali Al-Sahaf, Ali Al-Doweriej, Faisal Bayoumi, Leo L M Poon, Daniel K W Chu, Malik Peiris
BACKGROUND: Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans. METHODS: This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014-2016)...
October 6, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28993122/characterization-of-novel-monoclonal-antibodies-against-the-mers-coronavirus-spike-protein-and-their-application-in-species-independent-antibody-detection-by-competitive-elisa
#12
Shuetsu Fukushi, Aiko Fukuma, Takeshi Kurosu, Shumpei Watanabe, Masayuki Shimojima, Kazuya Shirato, Naoko Iwata-Yoshikawa, Noriyo Nagata, Kazuo Ohnishi, Manabu Ato, Simenew Keskes Melaku, Hiroshi Sentsui, Masayuki Saijo
Since discovering the Middle East respiratory syndrome coronavirus (MERS-CoV) as a causative agent of severe respiratory illness in the Middle East in 2012, serological testing has been conducted to assess antibody responses in patients and to investigate the zoonotic reservoir of the virus. Although the virus neutralization test is the gold standard assay for MERS diagnosis and for investigating the zoonotic reservoir, it uses live virus and so must be performed in high containment laboratories. Competitive ELISA (cELISA), in which a labeled monoclonal antibody (MAb) competes with test serum antibodies for target epitopes, may be a suitable alternative because it detects antibodies in a species-independent manner...
October 6, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28992542/de-novo-transcriptome-sequencing-of-camellia-sasanqua-and-the-analysis-of-major-candidate-genes-related-to-floral-traits
#13
Hui Huang, En-Hua Xia, Hai-Bin Zhang, Qiu-Yang Yao, Li-Zhi Gao
Camellia sasanqua is one of the most famous horticultural plants in Camellia (Theaceae) due to its aesthetic appeal as landscape plant. Knowledge regarding the genetic basis of flowering time, floral aroma and color in C. sasanqua is limited, but is essential to breed new varieties with desired floral traits. Here, we described the de novo transcriptome of young leaves, flower buds and flowers of C. sasanqua. A total of 60,127 unigenes were functionally annotated based on the sequence similarity. After analysis, we found that two floral integrator genes, SOC1 and AP1, in flowering time pathway showed evidence of gene family expansion...
September 1, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28992017/digestible-metabolizable-and-net-energy-of-camelina-cake-fed-to-growing-pigs-and-additivity-of-energy-in-mixed-diets
#14
J W Kim, B Koo, C M Nyachoti
This experiment was conducted to determine the DE, ME, and NE contents of camelina cake (CC) and to test the hypothesis that dietary glucosinolates originating from CC will affect the additivity of energy in mixed diets containing different inclusion levels of corn, soybean meal (SBM), and CC. A total of 30 growing barrows ([Yorkshire × Landrace] × Duroc) with a mean BW of 16.8 kg (SD 1.4) were randomly allotted to 1 of 5 treatments with 6 replicates per treatment. Pigs were fed experimental diets for 16 d, including 10 d for adaptation and 6 d for total collection of feces and urine...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28990656/optimization-of-saponin-extraction-conditions-with-camellia-sinensis-var-assamica-seed-and-its-application-for-natural-detergent
#15
Wanying Gong, Huang Yewei, Aibing Ji, Wenshu Peng, Cong Liu, Ying Zeng, Ruijuan Yang, Liang Yan, Xuanjun Wang, Jun Sheng
BACKGROUND: The Camellia sinensis var. assamica seed cake (by-product of tea-seed oil) is an abundant resource with poor utilization. The C. sinensis var. assamica seed saponin (CSS) is one kind of nonionic surfactant. In this study, The CSS extraction conditions were optimized by response surface methodology (RSM) and then the CSS detergent was developed. Additionally, the safety and decontamination ability of the developed detergent were evaluated. RESULTS: The optimized extraction conditions were including the extracting temperature of 40...
October 9, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28989935/pathologic-findings-of-chronic-pml-iris-in-a-patient-with-prolonged-pml-survival-following-natalizumab-treatment
#16
Mai Himedan, Sandra Camelo-Piragua, Elizabeth A Mills, Avneesh Gupta, Rany Aburashed, Yang Mao-Draayer
Immune reconstitution inflammatory syndrome (IRIS) is a common complication during treatment for natalizumab-associated progressive multifocal leukoencephalopathy (PML). Although severe IRIS can result in acute worsening of disability and is associated with poor prognosis, effective immune reconstitution may account for the high survival rate of this cohort of PML patients. We present pathological evidence of chronic IRIS 3.5 years after diagnosis with natalizumab-associated PML. Our case showed that the IRIS initially developed after plasma exchange therapy and resolved clinically and radiologically following a combination treatment with corticosteroids, maraviroc, and cidofovir...
July 2017: Journal of Investigative Medicine High Impact Case Reports
https://www.readbyqxmd.com/read/28989079/impact-of-aging-on-distribution-of-iga-and-igg-cells-in-aggregated-lymphoid-nodules-area-in-abomasum-of-bactrian-camels-camelus-bactrianus
#17
Wang-Dong Zhang, Xue-Feng Zhang, Cui-Cui Cheng, Shuai Jia, Lei Liu, Wen-Hui Wang
The aggregated lymphoid nodules area (ALNA) in the abomasum is a special organized lymphoid tissue discovered only in Bactrian camels at present. This study aimed to explore the impact of aging on distribution of IgA(+) and IgG(+) cells in ALNA in abomasum of Bactrian camels. Twenty-four Alashan Bactrian camels were divided into the following four age groups: young (1-2years), pubertal (3-5years), middle-aged (6-16years) and old (17-20years). IgA(+) and IgG(+) cells in the lamina propria of ALNA were observed and analyzed using immunohistochemical and statistical techniques...
October 5, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28987247/impact-of-autologous-stem-cell-transplantation-on-blood-pressure-and-renal-function-in-multiple-myeloma-patients
#18
Leah Balsam, Chadi Saad, Camelia Arsene, Joshua Fogel
BACKGROUND: Autologous stem cell transplantation (ASCT) reverses kidney failure in one-third of multiple myeloma (MM) patients, which may lead to blood pressure (BP) improvement. We evaluate the long term impact of ASCT on BP and renal function in MM patients. METHODS: We studied 192 MM patients that underwent ASCT. We compared BP readings and glomerular filtration rate (GFR) at 4 weeks before ASCT, on day of ASCT and post-ASCT at 30, 100 and 180 days. RESULTS: Mean systolic blood pressure (SBP) and diastolic blood pressure (DBP) on day of ASCT and at both 30 and 100 days post-ASCT was significantly lower as compared to pre-ASCT SBP and DBP...
2017: Journal of the National Medical Association
https://www.readbyqxmd.com/read/28986243/construction-of-an-expression-vector-that-uses-the-aph-promoter-for-protein-expression-in-corynebacterium-glutamicum
#19
Wei Zhang, Zihao Zhao, Yankun Yang, Xiuxia Liu, Zhonghu Bai
Corynebacterium glutamicum is an attractive host for the production of heterologous proteins despite its traditional use in fermentative production of amino acids. To enhance the expression levels of target genes, the development of useful promoters is required in the construction of expression systems. Here, we developed a new promoter, the aph promoter from aminoglycoside-3'-phosphotransferase gene, and used it to construct monocistronic and bicistronic expression systems that host different ribosome binding site (RBS) sequences...
October 3, 2017: Plasmid
https://www.readbyqxmd.com/read/28982028/effect-of-age-and-castration-on-serum-anti-m%C3%A3-llerian-hormone-concentration-in-male-alpacas
#20
Michela Ciccarelli, Ahmed Tibary, Alexis J Campbell, Alan J Conley
The synthesis of anti-Müllerian Hormone (AMH) by the Sertoli cells in males is crucial for sexual differentiation. There are no studies on AMH in Camelids. The objectives of this research were to 1) compare AMH serum concentrations in prepubertal and adult male alpacas and 2) determine the effect of castration on these concentrations in adult males to provide a validation of a commercial AMH test in alpacas. Serum samples were obtained from 15 prepubertal animals (5 for each age groups of 6, 7 and 8 months) and from 5 adult males (age 5-9 years), pre- and 24 h post-castration...
September 28, 2017: Theriogenology
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