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https://www.readbyqxmd.com/read/27918495/characteristics-of-nh4-and-no3-fluxes-in-tea-camellia-sinensis-roots-measured-by-scanning-ion-selective-electrode-technique
#1
Li Ruan, Kang Wei, Liyuan Wang, Hao Cheng, Fen Zhang, Liyun Wu, Peixian Bai, Chengcai Zhang
As a vital beverage crop, tea has been extensively planted in tropical and subtropical regions. Nitrogen (N) levels and forms are closely related to tea quality. Based on different N levels and forms, we studied changes in NO3(-) and NH4(+) fluxes in tea roots utilizing scanning ion-selective electrode technique. Our results showed that under both single and mixed N forms, influx rates of NO3(-) were much lower than those of NH4(+), suggesting a preference for NH4(+) in tea. With the increase in N concentration, the influx rate of NO3(-) increased more than that of NH4(+)...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27917989/cost-effective-control-strategies-for-animal-and-zoonotic-diseases-in-pastoralist-populations
#2
J Zinsstag, M F Abakar, M Ibrahim, R Tschopp, L Crump, B Bonfoh, E Schelling
Animal diseases and zoonoses abound among pastoralist livestock, which is composed of cattle, sheep, goats, yak, camels, llamas, reindeer, horses and donkeys. There is endemic and, periodically, epidemic transmission of highly contagious viral and bacterial diseases in Africa, Asia and Latin America. Pastoralist livestock is often multiparasitised with endo- and ectoparasites, as well as being affected by vectorborne viral and protozoal diseases. Pastoral livestock can be a reservoir of such diseases and can also, conversely, be at risk from exposure to wildlife reservoirs...
November 2016: Revue Scientifique et Technique
https://www.readbyqxmd.com/read/27917276/preparation-and-characterization-of-a-novel-nanobody-against-t-cell-immunoglobulin-and-mucin-3-tim-3
#3
Vida Homayouni, Mazdak Ganjalikhani-Hakemi, Abbas Rezaei, Hossein Khanahmad, Mahdi Behdani, Fatemeh Kazemi Lomedasht
OBJECTIVES: As T-cell immunoglobulin and mucin domain 3 (TIM-3) is an immune regulatory molecule; its blocking or stimulating could alter the pattern of immune response towards a desired condition. Based on the unique features of nanobodies, we aimed to construct an anti-TIM-3 nanobody as an appropriate tool for manipulating immune responses for future therapeutic purposes. MATERIALS AND METHODS: We immunized a camel with TIM-3 antigen and then, synthesized a VHH phage: mid library from its B cell's transcriptome using nested PCR...
November 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27916250/viral-serological-and-molecular-data-on-possible-involvement-of-herpes-viruses-in-periodontal-disease
#4
Cristiana Antipa, Coralia Bleotu, Camelia Grancea, Andreea Oana Rosu, Gabriela Anton, Simona Ruta
BACKGROUND: Recent studies have suggested that latent herpes virus infections can be associated with chronic periodontal sites that exhibit a predisposition to disease progression. The aim of this study was to identify the possible relationship between infections with CMV and EBV and the severity of periodontal disease. MATERIALS AND METHODS: Fifty two patients aged between 27 and 70 years, diagnosed with periodontal disease were enrolled in the study after giving informed consent...
December 2016: Singapore Dental Journal
https://www.readbyqxmd.com/read/27915048/protective-effects-of-green-tea-and-its-main-constituents-against-natural-and-chemical-toxins-a-comprehensive-review
#5
REVIEW
Maryam Rameshrad, Bibi Marjan Razavi, Hossein Hosseinzadeh
Toxins are natural or chemical poisonous substances with severe side effects on health. Humans are generally exposed by widespread toxic contaminations via air, soil, water, food, fruits and vegetables. Determining a critical antidote agent with extensive effects on different toxins is an ultimate goal for all toxicologists. Traditional medicine is currently perceived as a safe and natural approach against toxins. In this regard, we focused on the protective effects of green tea (Camellia sinensis) and its main components such as catechin, epicatechin, epicatechin gallate, gallocatechin, epigallocatechin and epigallocatechin gallate as a principal source of antioxidants against both natural and chemical toxins...
November 30, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27913814/life-course-socioeconomic-adversities-and-10-year-risk-of-cardiovascular-disease-cross-sectional-analysis-of-the-brazilian-longitudinal-study-of-adult-health
#6
Dayse Rodrigues de Sousa Andrade, Lidyane V Camelo, Rodrigo Citton P Dos Reis, Itamar S Santos, Antonio Luiz Ribeiro, Luana Giatti, Sandhi Maria Barreto
OBJECTIVES: To investigate whether life course exposure to adverse socioeconomic positions (SEP) as well as maintaining a low SEP or decreasing the SEP intra- and intergeneration was associated with an increased 10-year cardiovascular disease (CVD) risk predicted by the Framingham Risk Score. METHODS: This is a cross-sectional analysis of baseline data (2008-2010) of 13,544 active workers from ELSA-Brasil cohort. Maternal education, leg length, social class of first occupation and education were used to evaluate childhood, youth and adulthood SEP...
December 2, 2016: International Journal of Public Health
https://www.readbyqxmd.com/read/27908701/inhibitory-effects-of-l-theanine-on-airway-inflammation-in-ovalbumin-induced-allergic-asthma
#7
Yong Pil Hwang, Sun Woo Jin, Jae Ho Choi, Chul Yung Choi, Hyung Gyun Kim, Se Jong Kim, Yongan Kim, Kyung Jin Lee, Young Chul Chung, Hye Gwang Jeong
L-theanine, a water-soluble amino acid isolated from green tea (Camellia sinensis), has anti-inflammatory activity, antioxidative properties, and hepatoprotective effects. However, the anti-allergic effect of L-theanine and its underlying molecular mechanisms have not been elucidated. In this study, we investigated the protective effects of L-theanine on asthmatic responses, particularly airway inflammation and oxidative stress modulation in an ovalbumin (OVA)-induced murine model of asthma. Treatment with L-theanine dramatically attenuated the extensive trafficking of inflammatory cells into bronchoalveolar lavage fluid (BALF)...
November 28, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27905655/-gastrointestinal-bezoars
#8
Ricardo Espinoza González
Gastrointestinal bezoars are a concretion of indigested material that can be found in the gastrointestinal tract of humans and some animals. This material forms an intraluminal mass, more commonly located in the stomach. During a large period of history animal bezoars were considered antidotes to poisons and diseases. We report a historical overview since bezoars stones were thought to have medicinal properties. This magic conception was introduced in South America by Spanish conquerors. In Chile, bezoars are commonly found in a camelid named guanaco (Lama guanicoe)...
August 2016: Revista Médica de Chile
https://www.readbyqxmd.com/read/27903650/structural-analysis-of-single-domain-antibodies-bound-to-a-second-neutralizing-hot-spot-on-ricin-toxin-s-enzymatic-subunit
#9
Michael J Rudolph, David J Vance, Michael S Cassidy, Yinghui Rong, Nicholas J Mantis
Ricin toxin is a heterodimer consisting of RTA, a ribosome-inactivating protein, and RTB, a lectin that facilitates receptor-mediated uptake into mammalian cells. In previous studies, we demonstrated that toxin-neutralizing antibodies target four spatially distinct hotspots on RTA, which we refer to as epitope Clusters I-IV. In this report we identified and characterized three single domain camelid antibodies (VHH) against Cluster II. One of these VHHs, V5E1, ranks as one of the most potent ricin-neutralizing antibodies described to date...
November 30, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27902299/chryseobacterium-nepalense-sp-nov-isolated-from-oil-contaminated-soil
#10
Dhiraj Kumar Chaudhary, Jaisoo Kim
During the study of hydrocarbon-degrading bacteria in the oil-contaminated soil of Biratnagar, Morang, Nepal, a yellow-coloured, Gram-staining negative, aerobic, non-motile, and rod shaped bacterium, designated strain C-5-3T, was isolated. This strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain C-5-3T belongs to the genus Chryseobacterium and is closely related to 'Chryseobacterium profundimaris' DY46 (98.19% sequence similarity), Chryseobacterium takakiae AG1-2T (98...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27900272/return-to-physical-activity-after-gastrocnemius-recession
#11
Camelia Tang Qian Ying, Sean Lai Wei Hong, Bing Howe Lee, Gowreeson Thevendran
AIM: To prospectively investigate the time taken and patients' ability to resume preoperative level of physical activity after gastrocnemius recession. METHODS: Endoscopic gastrocnemius recession (EGR) was performed on 48 feet in 46 consecutive sportspersons, with a minimum follow-up of 24 mo. The Halasi Ankle Activity Score was used to quantify the level of physical activity. Time taken to return to work and physical activity was recorded. Functional outcomes were evaluated using the short form 36 (SF-36), American Orthopedic Foot and Ankle Society (AOFAS) Hindfoot score and modified Olerud and Molander (O and M) scores respectively...
November 18, 2016: World Journal of Orthopedics
https://www.readbyqxmd.com/read/27898078/corrigendum-genetic-variants-associated-with-subjective-well-being-depressive-symptoms-and-neuroticism-identified-through-genome-wide-analyses
#12
Aysu Okbay, Bart M L Baselmans, Jan-Emmanuel De Neve, Patrick Turley, Michel G Nivard, Mark Alan Fontana, S Fleur W Meddens, Richard Karlsson Linnér, Cornelius A Rietveld, Jaime Derringer, Jacob Gratten, James J Lee, Jimmy Z Liu, Ronald de Vlaming, Tarunveer S Ahluwalia, Jadwiga Buchwald, Alana Cavadino, Alexis C Frazier-Wood, Nicholas A Furlotte, Victoria Garfield, Marie Henrike Geisel, Juan R Gonzalez, Saskia Haitjema, Robert Karlsson, Sander W van der Laan, Karl-Heinz Ladwig, Jari Lahti, Sven J van der Lee, Penelope A Lind, Tian Liu, Lindsay Matteson, Evelin Mihailov, Michael B Miller, Camelia C Minica, Ilja M Nolte, Dennis Mook-Kanamori, Peter J van der Most, Christopher Oldmeadow, Yong Qian, Olli Raitakari, Rajesh Rawal, Anu Realo, Rico Rueedi, Börge Schmidt, Albert V Smith, Evie Stergiakouli, Toshiko Tanaka, Kent Taylor, Gudmar Thorleifsson, Juho Wedenoja, Juergen Wellmann, Harm-Jan Westra, Sara M Willems, Wei Zhao, Najaf Amin, Andrew Bakshi, Sven Bergmann, Gyda Bjornsdottir, Patricia A Boyle, Samantha Cherney, Simon R Cox, Gail Davies, Oliver S P Davis, Jun Ding, Nese Direk, Peter Eibich, Rebecca T Emeny, Ghazaleh Fatemifar, Jessica D Faul, Luigi Ferrucci, Andreas J Forstner, Christian Gieger, Richa Gupta, Tamara B Harris, Juliette M Harris, Elizabeth G Holliday, Jouke-Jan Hottenga, Philip L De Jager, Marika A Kaakinen, Eero Kajantie, Ville Karhunen, Ivana Kolcic, Meena Kumari, Lenore J Launer, Lude Franke, Ruifang Li-Gao, David C Liewald, Marisa Koini, Anu Loukola, Pedro Marques-Vidal, Grant W Montgomery, Miriam A Mosing, Lavinia Paternoster, Alison Pattie, Katja E Petrovic, Laura Pulkki-Råback, Lydia Quaye, Katri Räikkönen, Igor Rudan, Rodney J Scott, Jennifer A Smith, Angelina R Sutin, Maciej Trzaskowski, Anna E Vinkhuyzen, Lei Yu, Delilah Zabaneh, John R Attia, David A Bennett, Klaus Berger, Lars Bertram, Dorret I Boomsma, Harold Snieder, Shun-Chiao Chang, Francesco Cucca, Ian J Deary, Cornelia M van Duijn, Johan G Eriksson, Ute Bültmann, Eco J C de Geus, Patrick J F Groenen, Vilmundur Gudnason, Torben Hansen, Catharine A Hartman, Claire M A Haworth, Caroline Hayward, Andrew C Heath, David A Hinds, Elina Hyppönen, William G William, Marjo-Riitta Järvelin, Karl-Heinz Jöckel, Jaakko Kaprio, Sharon L R Kardia, Liisa Keltikangas-Järvinen, Peter Kraft, Laura D Kubzansky, Terho Lehtimäki, Patrik K E Magnusson, Nicholas G Martin, Matt McGue, Andres Metspalu, Melinda Mills, Renée de Mutsert, Albertine J Oldehinkel, Gerard Pasterkamp, Nancy L Pedersen, Robert Plomin, Ozren Polasek, Christine Power, Stephen S Rich, Frits R Rosendaal, Hester M den Ruijter, David Schlessinger, Helena Schmidt, Rauli Svento, Reinhold Schmidt, Behrooz Z Alizadeh, Thorkild I A Sørensen, Tim D Spector, John M Starr, Kari Stefansson, Andrew Steptoe, Antonio Terracciano, Unnur Thorsteinsdottir, A Roy Thurik, Nicholas J Timpson, Henning Tiemeier, André G Uitterlinden, Peter Vollenweider, Gert G Wagner, David R Weir, Jian Yang, Dalton C Conley, George Davey Smith, Albert Hofman, Magnus Johannesson, David I Laibson, Sarah E Medland, Michelle N Meyer, Joseph K Pickrell, Tõnu Esko, Robert F Krueger, Jonathan P Beauchamp, Philipp D Koellinger, Daniel J Benjamin, Meike Bartels, David Cesarini
No abstract text is available yet for this article.
November 29, 2016: Nature Genetics
https://www.readbyqxmd.com/read/27898032/the-antiproliferative-effect-of-chakasaponins-i-and-ii-floratheasaponin-a-and-epigallocatechin-3-o-gallate-isolated-from-camellia-sinensis-on-human-digestive-tract-carcinoma-cell-lines
#13
Niichiro Kitagawa, Toshio Morikawa, Chiaki Motai, Kiyofumi Ninomiya, Shuhei Okugawa, Ayaka Nishida, Masayuki Yoshikawa, Osamu Muraoka
Acylated oleanane-type triterpene saponins, namely chakasaponins I (1) and II (2), floratheasaponin A (3), and their analogs, together with catechins-including (-)-epigallocatechin 3-O-gallate (4), flavonoids, and caffeine-have been isolated as characteristic functional constituents from the extracts of "tea flower", the flower buds of Camellia sinensis (Theaceae), which have common components with that of the leaf part. These isolates exhibited antiproliferative activities against human digestive tract carcinoma HSC-2, HSC-4, MKN-45, and Caco-2 cells...
November 26, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27897996/in-vitro-chemopreventive-properties-of-green-tea-rooibos-and-honeybush-extracts-in-skin-cells
#14
Tandeka U Magcwebeba, Pieter Swart, Sonja Swanevelder, Elizabeth Joubert, Wentzel C A Gelderblom
The chemopreventive properties of the herbal teas rooibos (Aspalathus linearis) and honeybush (Cyclopia spp.) have been demonstrated on mouse skin in vivo but the underlying mechanisms are not clear. The aim of the current study was to determine the anti-proliferative and pro-apoptotic activity of methanol and aqueous extracts of rooibos and two Cyclopia species in different skin cells, using green tea (Camellia sinensis) as a benchmark. Extracts were also characterised for their major individual polyphenols by high performance liquid chromatography and spectroscopically for the total polyphenol (TP) groups...
November 25, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27894568/gender-differences-in-cumulative-life-course-socioeconomic-position-and-social-mobility-in-relation-to-new-onset-diabetes-in%C3%A2-adults-the-brazilian-longitudinal-study-of-adult-health-elsa-brasil
#15
Lidyane V Camelo, Luana Giatti, Bruce B Duncan, Dóra Chor, Rosane Härter Griep, Maria Inês Schmidt, Sandhi Maria Barreto
PURPOSE: We investigated gender-specific associations of cumulative socioeconomic position across life course and social mobility with new onset diabetes mellitus (NODM) in over 12,000 civil servants in Brazil. METHODS: We used data from ELSA-Brasil baseline (2008-2010). The accumulation of risk was assessed using an education-based score and an occupation-based score. Educational and occupational social mobility were also evaluated. RESULTS: In minimally adjusted models, NODM increased with increasing exposure to life-course social disadvantages, especially in men...
December 2016: Annals of Epidemiology
https://www.readbyqxmd.com/read/27888728/estimating-total-lipid-content-of-camelina-sativa-via-pyrolysis-assisted-in-situ-transesterification-with-dimethyl-carbonate
#16
Jong-Min Jung, Jechan Lee, Jeong-Ik Oh, Hyung-Wook Kim, Eilhann E Kwon
Direct derivatization of C. sativa seed into FAMEs without lipid extraction was conducted for the quantification of lipid analysis via in-situ thermal methylation with dimethyl carbonate as an acyl acceptor on silica (SiO2). The introduced method had an extraordinarily high tolerance against impurities such as pyrolytic products and moisture. To ensure the technical completeness of in-situ methylation, thermal cracking of FAMEs transformed from C. sativa seed was also explored. Thermal cracking of unsaturated FAMEs such as C18:1, C18:2, C18:3 and C20:1 occurred at temperatures higher than 365°C due to their thermal instability...
November 16, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27885771/selective-gene-dosage-by-crispr-cas9-genome-editing-in-hexaploid-camelina-sativa
#17
Céline Morineau, Yannick Bellec, Frédérique Tellier, Lionel Gissot, Zsolt Kelemen, Fabien Nogué, Jean-Denis Faure
In many plant species, gene dosage is an important cause of phenotype variation. Selectively engineering gene dosage, particularly in polyploid genomes, would provide an efficient tool for plant breeding. The hexaploid oilseed crop Camelina sativa, which has three closely-related expressed sub-genomes, is an ideal species for investigation of the possibility of creating a large collection of combinatorial mutants. Selective, targeted mutagenesis of the three delta-12-desaturase (FAD2) genes was achieved by CRISPR-Cas9 gene editing, leading to reduced levels of polyunsaturated fatty acids and increased accumulation of oleic acid in the oil...
November 25, 2016: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/27884489/geochemical-speciation-bioavailability-and-source-identification-of-selected-metals-in-surface-sediments-of-the-southern-caspian-sea
#18
Kazem Darvish Bastami, Mahmoud Reza Neyestani, Marjan Esmaeilzadeh, Sarah Haghparast, Camelia Alavi, Sanaz Fathi, Shahram Nourbakhsh, Emam Ali Shirzadi, Reyhane Parhizgar
Geochemical speciation of As, Cd, Co, Cr, Cu, Ni, Pb, V and Zn were determined in the surface sediments of the southern Caspian Sea. A five-step sequential extraction technique was used to determine the chemical forms of metals. Mean concentrations (ppm) of heavy metals were (mean±S.D.) As: 9.94±1.71, Cd: 0.87±0.23, Co: 14.85±2.80, Cr: 72.29±19.48, Cu: 18.91±4.48, Ni: 32.87±5.25, Pb: 12.48±3.22, V: 86.07±20.71 and Zn: 66.85±10.11. Among the metals, Cu, As, Pb and Zn exhibited relatively higher mobility, while Cd, Co, Cr, Ni and V were found mainly in the residual fractions...
November 21, 2016: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/27883942/dye-degradation-by-green-heterogeneous-fenton-catalysts-prepared-in-presence-of-camellia-sinensis
#19
Samira S F Carvalho, Nakédia M F Carvalho
This work describes the synthesis and characterization of supported green iron catalysts, prepared with Camellia sinensis tea extract, and their application in heterogeneous Fenton degradation of pollutant dyes. The influence of the catalyst synthesis conditions in the iron and organic content were investigated by X-ray fluorescence and thermogravimetric analyses. Irregular, chain-like nanoparticles, in the size range of 20-100 nm, capped by polyphenolic natural compounds, were visualized by TEM micrographs...
November 21, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27882637/transplantation-associated-thrombotic-microangiopathy-isolated-to-a-congenital-anomaly-of-the-lung
#20
Rebecca T Kummen, Geoffrey D E Cuvelier, Camelia Stefanovici, Anamarija M Perry, Rick Higgins, Rochelle Yanofsky, Suyin A Lum Min, Donna A Wall
TA-TMA is a post-hematopoietic stem cell transplant complication with clinical features of hemolytic anemia and thrombocytopenia. A 26-month-old child who had had an allogeneic transplant for treatment of DBA developed severe TA-TMA with heavy red blood cell and platelet transfusion dependence. Incidentally, he was found to have a lung sequestration. TA-TMA resolved and transfusion dependence resolved after resection of the sequestration. The finding suggests the malformation vasculature was selectively vulnerable to the trigger of TA-TMA-raising perhaps a clue to basic pathophysiology of TA-TMA and/or vascular malformations...
November 24, 2016: Pediatric Transplantation
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