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https://www.readbyqxmd.com/read/30221698/theanine-an-antitumor-promoter-induces-apoptosis-of-tumor-cells-via-the-mitochondrial-pathway
#1
Yinqiang Xin, Peiling Ben, Qi Wang, Yanyan Zhu, Zhimin Yin, Lan Luo
Theanine, an active component of green tea (Camellia sinensis), is considered a modulator of chemotherapy. To further investigate the anticancer activity of theanine, the present study investigated the cytotoxic effect of theanine at the concentration of 600 µg/ml, in the human HepG2 hepatoblastoma and HeLa adenocarcinoma cell lines, in comparison with the normal L02, H9c2 and HEK293 cell lines using a MTT assay. It was found that theanine induced cell death in the tumor cells, but not in the normal cells...
September 5, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/30219555/coxiella-burnetii-in-tunisian-dromedary-camels-camelus-dromedarius-seroprevalence-associated-risk-factors-and-seasonal-dynamics
#2
Selmi Rachid, Mamlouk Aymen, Ben Yahia Houcine, Abdelaali Hedi, Ben Said Mourad, Sellami Khalil, Daaloul-Jedidi Monia, Jemli Mohamed Habib, Messadi Lilia
Q fever, caused by Coxiella burnetii, is a zoonotic disease responsible of abortion in ruminants. Few studies have investigated the prevalence of this infection in camels (Camelus dromedarius). The present report aimed to highlight the epidemiological status and identify the risk factors associated with C. burnetii infection in one-humped dromedary that is the most productive livestock species in arid areas. A total of 534 sera of healthy camels were collected in eight governorates from southern and central Tunisia...
September 13, 2018: Acta Tropica
https://www.readbyqxmd.com/read/30214218/rare-complications-of-neurofibromatosis-1-diagnosed-incidentally-in-two-children
#3
Cecilia Lazea, Carmen Asavoaie, Camelia Al-Khzouz, Lenuta Popa
Neurofibromatosis 1 (NF1) is an autosomal-dominant disorder with various clinical expressivity and complications. Arterial hypertension may be present in patients with NF1 and is secondary to vasculopathy or pheochromocytoma. We herein describe two children (17 and 4 years old) diagnosed late with NF1 after severe arterial hypertension had been discovered due to pheochromocytoma and middle aortic syndrome. Routine measurement of arterial pressure is mandatory in children with NF1, in order to diagnose the complications of this disease...
2018: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/30213988/a-novel-method-for-detection-of-camellia-oil-adulteration-based-on-time-resolved-emission-fluorescence
#4
Hui Chen, Bin Chen, Daoli Lu
In this study, time-resolved emission fluorescence (TRES) combined with chemometrics was developed and employed for adulteration analysis of camellia oil. TRES was first decomposed by parallel factors analysis (PARAFAC). Next, an artificial neural network (ANN) model was built for the adulteration analysis. A linear range of 5-50%, a limit of detection (LOD) of 3% and root mean square error of prediction (RMSEP) values lower than 3% were achieved. Compared with the steady-state measurement, easy access to the information from fluorophores of low concentration was shown to be an intrinsic advantage of the time-resolved measurement; this advantageous characteristic was helpful for optimizing adulteration analysis...
September 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30213684/more-than-just-caffeine-psychopharmacology-of-methylxanthine-interactions-with-plant-derived-phytochemicals
#5
REVIEW
Julius Schuster, Ellen S Mitchell
In general, preparations of coffee, teas, and cocoa containing high levels of polyphenols, L-theanine and other bioactive compounds selectively enhance mood and cognition effects of caffeine. This review summarizes the bioactive components of commonly consumed natural caffeine sources (e.g. guayusa, mate and camellia teas, coffee and cocoa) and analyzes the psychopharmacology of constituent phytochemicals: methylxanthines, polyphenols, and L-theanine. Acute and chronic synergistic effects of these compounds on mood and cognition are compared and discussed...
September 10, 2018: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/30213127/rapid-determination-of-the-oil-and-moisture-contents-in-camellia-gauchowensis-chang-and-camellia-semiserrata-chi-seeds-kernels-by-near-infrared-reflectance-spectroscopy
#6
Yingzhong Zhang, Liangbo Zhang, Jing Wang, Xuxiao Tang, Hong Wu, Minghuai Wang, Wu Zeng, Qihui Mo, Yongquan Li, Jianwei Li, Yijuan Huang, Baohua Xu, Mengyu Zhang
A fast and effective determination method of different species of vegetable seeds oil is vital in the plant oil industry. The near-infrared reflectance spectroscopy (NIRS) method was developed in this study to analyze the oil and moisture contents of Camellia gauchowensis Chang and C. semiserrata Chi seeds kernels. Calibration and validation models were established using principal component analysis (PCA) and partial least squares (PLS) regression methods. In the prediction models of NIRS, the levels of accuracy obtained were sufficient for C...
September 12, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30212327/gastric-pythiosis-in-a-bactrian-camel-bactrianus-camelus
#7
Lilian C Heck, Matheus V Bianchi, Paula R Pereira, Marina P Lorenzett, Cíntia de Lorenzo, Saulo P Pavarini, David Driemeier, Luciana Sonne
Pythium insidiosum, an aquatic oomycete, causes chronic lesions in the skin and digestive tract of multiple species. A captive-bred Bactrian camel ( Camelus bactrianus) showed clinical signs of lethargy and weight loss in a clinical course of 30 days, with no response to treatment. At necropsy, the abdominal cavity had approximately 32 L of a yellow, turbid fluid with fibrin. The third compartment of the stomach (C-3) showed a focal area of rupture covered with fibrin. Close to this area, the C-3 wall was thickened and firm, demonstrating irregular, yellow, and friable areas on cut surface (kunkers)...
September 2018: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/30209617/significantly-increased-amino-acid-accumulation-in-a-novel-albino-branch-of-the-tea-plant-camellia-sinensis
#8
Mengqian Lu, Jieyun Han, Biying Zhu, Huiyan Jia, Tianyuan Yang, Rangjian Wang, Wei-Wei Deng, Zheng-Zhu Zhang
A normal tea plant with one albino branch was discovered. RNA sequencing, albinism phenotype and ultrastructural observations provided a valuable understanding of the albino mechanism in tea plants. Tea plants with a specific color (white or yellow) have been studied extensively. A normal tea plant (Camellia sinensis cv. quntizhong) with one albino branch was discovered in a local tea plantation in Huangshan, Anhui, China. The pure albino leaves on this special branch had accumulated a fairly high content of amino acids, especially theanine (45...
September 12, 2018: Planta
https://www.readbyqxmd.com/read/30208034/isometamidium-chloride-and-homidium-chloride-fail-to-cure-mice-infected-with-ethiopian-trypanosoma-evansi-type-a-and-b
#9
Gebrekrustos Mekonnen, Elmi Fahiye Mohammed, Weldu Kidane, Awol Nesibu, Hagos Yohannes, Nick Van Reet, Philippe Büscher, Hadush Birhanu
BACKGROUND: Trypanosoma evansi is mechanically transmitted by biting flies and affects camels, equines, and other domestic and wild animals in which it causes a disease called surra. At least two types of Trypanosoma evansi circulate in Ethiopia: type A, which is present in Africa, Latin America and Asia, and type B, which is prevalent in Eastern Africa. Currently, no information is available about the drug sensitivity of any Ethiopian T. evansi type. METHODOLOGY/PRINCIPAL FINDINGS: This study was conducted with the objective of determining the in vivo drug sensitivity of two T...
September 12, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/30206849/-the-aggressive-patient
#10
REVIEW
Camelia-Lucia Cimpianu, Kolja Schiltz
No abstract text is available yet for this article.
September 2018: MMW Fortschritte der Medizin
https://www.readbyqxmd.com/read/30205092/structural-basis-of-epitope-recognition-by-heavy-chain-camelid-antibodies
#11
Uroš Zavrtanik, Junoš Lukan, Remy Loris, Jurij Lah, San Hadži
Truncated versions of heavy chain antibodies (HCAbs) from camelids, also termed nanobodies, comprise only one-tenth the mass of conventional antibodies, yet retain similar, high binding affinities for the antigens. Here we analyze a large dataset of nanobody-antigen crystal structures and investigate how nanobody-antigen recognition compares to the one by conventional antibodies. We find that nanobody paratopes are enriched in aromatic residues just like conventional antibodies, but additionally they also bear a more hydrophobic character...
September 8, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/30204982/prevalence-of-low-birth-weight-in-a-scenario-of-economic-depression-in-argentina
#12
Julia Ratowiecki, Fernando A Poletta, Lucas G Giménez, Juan A Gili, Mariela S Pawluk, Jorge S López Camelo
INTRODUCTION: Low birth weight (LBW) is considered a general indicator of health because it is related to complications in the life of a newborn infant and is one of the leading causes of infant mortality. It is a multifactorial indicator, and its determinants include socioeconomic factors. OBJECTIVE: To assess the impact of economic inequality on the prevalence of LBW by quantifying its differential effect by maternal age, level of maternal education, and level of care...
October 1, 2018: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/30204754/tick-borne-zoonoses-in-the-order-rickettsiales-and-legionellales-in-iran-a-systematic-review
#13
Faham Khamesipour, Gabriel O Dida, Douglas N Anyona, Mostafa Razavi, Ehsan Rakhshandehroo
BACKGROUND: Tick-borne zoonoses in the Order Rickettsiales and Legionellales cause infections that often manifest as undifferentiated fevers that are not easy to distinguish from other causes of acute febrile illnesses clinically. This is partly attributed to difficulty in laboratory confirmation since convalescent sera, specific diagnostic reagents, and the required expertise may not be readily available. As a result, a number of tick-borne zoonoses are underappreciated resulting in unnecessary morbidity, mortality and huge economic loses...
September 11, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/30203900/toxoplasma-gondii-neospora-caninum-and-encephalitozoon-cuniculi-in-animals-from-captivity-zoo-and-circus-animals
#14
Jiřina Marková, Tereza Machačová, Eva Bártová, Kamil Sedlák, Marie Budíková, Pasquale Silvestre, Paolo Laricchiuta, Marco Russo, Vincenzo Veneziano
Problems with parasitic infections are common in zoological gardens and circuses, and can lead in some animals to several disorders such as systemic disease, reproductive disorders (abortions and neonatal mortality), and even to death if severe illness is untreated. Thus, the aim of this study was to evaluate the prevalence of three parasites in 74 animals from three zoos, and four circuses in Southern Italy. Antibodies to Toxoplasma gondii, Neospora caninum and Encephalitozoon cuniculi were detected in 51%, 12 % and 20% of animals, respectively...
September 11, 2018: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/30203186/gametogony-of-eimeria-cameli-in-the-small-intestine-of-one-humped-camel-camelus-dromedarius
#15
Jitender P Dubey, Rolf K Schuster, Joerg Kinne
Domesticated Old World camels (Camelus dromedarius and Camelus bactrianus) are important for the economy of several countries in Asia, Africa, and the Arabian Peninsula, and coccidiosis is an important disease in camels. There is confusion concerning the species of coccidian parasites in camels and their life cycles. Although five species of Eimeria (E. cameli, E. rajasthani, E. dromedarii, E. bactriani, and E. pellerdyi) were named from camels, E. cameli is considered the most pathogenic. Here, development of gametogonic stages and oocysts of E...
September 10, 2018: Parasitology Research
https://www.readbyqxmd.com/read/30202972/screening-for-pfhrp2-3-deleted-plasmodium-falciparum-non-falciparum-and-low-density-malaria-infections-by-a-multiplex-antigen-assay
#16
Mateusz M Plucinski, Camelia Herman, Sophie Jones, Rafael Dimbu, Filomeno Fortes, Dragan Ljolje, Naomi Lucchi, Sean C Murphy, Nahum T Smith, Kurtis R Cruz, Annette M Seilie, Eric S Halsey, Venkatachalam Udhayakumar, Michael Aidoo, Eric Rogier
Background: Detection of Plasmodium antigens provides evidence of malaria infection status and is the basis for most malaria diagnosis worldwide. Methods: We developed a sensitive bead-based multiplex assay for laboratory use which simultaneously detects the pan-Plasmodium pAldo, pan-Plasmodium pLDH, and P. falciparum PfHRP2 antigens. The assay was validated against purified recombinant antigens, mono-species malaria infections, and non-infected blood samples. To test against samples collected in an endemic setting, Angolan outpatient samples (n=1267) were assayed...
September 7, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/30202703/magnetism-and-magnetoresistance-of-single-ni-cu-alloy-nanowires
#17
Andreea Costas, Camelia Florica, Elena Matei, Maria Eugenia Toimil-Molares, Ionel Stavarache, Andrei Kuncser, Victor Kuncser, Ionut Enculescu
Arrays of magnetic Ni-Cu alloy nanowires with different compositions were prepared by a template-replication technique using electrochemical deposition into polycarbonate nanoporous membranes. Photolithography was employed for obtaining interdigitated metallic electrode systems of Ti/Au onto SiO2 /Si substrates and subsequent electron beam lithography was used for contacting single nanowires in order to investigate their galvano-magnetic properties. The results of the magnetoresistance measurements made on single Ni-Cu alloy nanowires of different compositions have been reported and discussed in detail...
2018: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/30200194/assessing-animal-welfare-in-animal-visitor-interactions-in-zoos-and-other-facilities-a-pilot-study-involving-giraffes
#18
Simona Normando, Ilaria Pollastri, Daniela Florio, Linda Ferrante, Elisabetta Macchi, Valentina Isaja, Barbara de Mori
In recent years, awareness of the controversial aspects connected with wild animal-visitor interactions (AVIs) in zoos and other facilities has increased due to cultural changes. Therefore, the need to apply transparent procedures to evaluate AVIs programs in zoos and similar facilities has also increased. This study presents results of animal welfare's assessment of a pilot test of a protocol based on six steps that aim to explore and assess the overall value of AVIs considering the impact both on animals and visitors...
August 30, 2018: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/30198713/metabolomics-and-transcriptomics-analyses-reveal-nitrogen-influences-on-the-accumulation-of-flavonoids-and-amino-acids-in-young-shoots-of-tea-plant-camellia-sinensis-l-associated-with-tea-flavor
#19
Hui Huang, Qiuyang Yao, Enhua Xia, Lizhi Gao
Tea-specialized metabolites contribute to rich flavors and healthy function of tea. Their accumulation patterns and underlying regulatory mechanism are significantly different under different nitrogen (N) conditions during adaptation stage. Here, we find that flavonoids associated with tea flavor are dominated by different metabolic and transcriptional responses among the four N conditions (N-deficiency, nitrate, ammonia, and nitric oxide). Nitrogen-deficiency tea plants accumulate diverse flavonoids, corresponding with higher expression of hub genes including F3H, FNS, UFGT, bHLH35, and bHLH36...
September 10, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/30197015/development-and-validation-of-direct-dry-loop-mediated-isothermal-amplification-for-diagnosis-of-trypanosoma-evansi
#20
Bashir Salim, Kyoko Hayashida, Ehab Mossaad, Ryo Nakao, Junya Yamagishi, Chihiro Sugimoto
Non-tsetse transmitted Trypanosoma evansi infection (Surra) is one of the most important diseases of camels in north and east Africa and of buffalo and cattle in Asia. Early, accurate and feasible diagnosis is a crucial step towards the control of Surra. Dry format of loop-mediated isothermal amplification (LAMP) diagnostics for the detection of T. evansi was developed, where the detection limit was determined as to equivalent to one parasite per reaction. The assay was validated by testing blood from 48 camels clinically diagnosed to have Surra, which all tested negative microscopically and revealed 43 (89...
August 30, 2018: Veterinary Parasitology
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