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https://www.readbyqxmd.com/read/29457451/boosted-electrochemical-immunosensing-gm-crops-markers-using-nanobody-and-mesoporous-carbon
#1
Mingming Zhang, Guanghui Li, Qing Zhou, Deng Pan, Min Zhu, Runyu Xiao, Yuanjian Zhang, Guoqiu Wu, Yakun Wan, Yanfei Shen
The problems of environmental security and the potential risks of human health caused by transgenic crops have aroused numerous attention. Recent studies reveal 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Agrobacterium sp. strain CP4 protein (CP4-EPSPS), which shows very high resistance to herbicide glyphosate, is a typical biomarker of genetically modified (GM) crops. For this reason, it is highly anticipated to devise a sensitive and convenient strategy to detect CP4-EPSPS protein in crops. Herein, we reported a simple electrochemical immunosensor by coupling nanobody, ordered mesoporous carbon (OMC), and thionine (Th)...
February 19, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29456258/modifying-the-standard-disk-model-for-the-ultraviolet-spectral-analysis-of-disk-dominated-cataclysmic-variables-i-the-novalikes-mv-lyrae-bz-camelopardalis-and-v592-cassiopeiae
#2
Patrick Godon, Edward M Sion, Şölen Balman, William P Blair
The standard disk is often inadequate to model disk-dominated cataclysmic variables (CVs) and generates a spectrum that is bluer than the observed UV spectra. X-ray observations of these systems reveal an optically thin boundary layer (BL) expected to appear as an inner hole in the disk. Consequently, we truncate the inner disk. However, instead of removing the inner disk, we impose the no-shear boundary condition at the truncation radius, thereby lowering the disk temperature and generating a spectrum that better fits the UV data...
September 1, 2017: Astrophysical Journal
https://www.readbyqxmd.com/read/29454297/intracytoplasmic-sperm-injection-icsi-of-in-vitro-matured-oocytes-with-stored-epididymal-spermatozoa-in-camel-camelus-dromedarius-effect-of-exogenous-activation-on-in-vitro-embryo-development
#3
Nisar A Wani, Seungbum Hong
Experiments were conducted to investigate the development of in vitro matured camel oocytes after their intra-cytoplasmic sperm injection (ICSI) with epididymal sperm collected from slaughtered male camels. Ovaries and testes were collected from a local slaughterhouse in normal saline solution (NSS) at 37 °C and on ice (0-1 °C), respectively. Cumulus-oocyte complexes (COCs) aspirated from the follicles were randomly distributed to 4-well culture plates (20-25 COCs/well) containing 500 μL of maturation medium and cultured at 38...
February 8, 2018: Theriogenology
https://www.readbyqxmd.com/read/29453744/low-serum-ige-is-a-sensitive-and-specific-marker-for-common-variable-immunodeficiency-cvid
#4
Monica G Lawrence, Thamiris V Palacios-Kibler, Lisa J Workman, Alexander J Schuyler, John W Steinke, Spencer C Payne, Emily C McGowan, James Patrie, Ramsay L Fuleihan, Kathleen E Sullivan, Patricia L Lugar, Camellia L Hernandez, Douglas E Beakes, James W Verbsky, Thomas A E Platts-Mills, Charlotte Cunningham-Rundles, John M Routes, Larry Borish
Although small prior studies have suggested that IgE can be low in common variable immunodeficiency (CVID), the workup for patients with recurrent infections and suspected hypogammaglobulinemia does not include the routine measurement of serum IgE. We sought to test the hypothesis that low/undetectable serum IgE is characteristic of CVID by comparing the frequency of low/undetectable serum IgE in healthy controls and patients with CVID. We measured total serum IgE in a large multi-center cohort of patients with CVID (n = 354) and compared this to large population-based cohorts of children and adults...
February 17, 2018: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/29450815/factors-affecting-reproductive-performance-in-dromedary-camel-herds-in-saudi-arabia
#5
Ahmed Ali, Derar Derar, Abdulhadi Alsharari, Assaf Alsharari, Rashid Khalil, Tariq I Almundarij, Yaser Alboti, Fahd Al-Sobayil
A survey of 7122 dromedary camels in 115 herds in Saudi Arabia was used to estimate the effects of herd size (HZ; < 25 vs. 25-49 vs. 50-100 vs. > 100 camels), herder/camels ratio (H/C; 1:< 25 vs. 1:25-50 vs. 1:> 50), manager experience (ME; < 5 vs. 5-10 vs. > 10 years), male/females ratio (M/F), housing system (HS; free vs. closed vs. mixed), length of the breeding season (winter vs. winter and spring vs. fall, winter and spring), age at first mating (3 vs. > 3 years), and time of mating after parturition (≤ 3 vs...
February 15, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29450383/eyesight-quality-and-computer-vision-syndrome
#6
Camelia Margareta Bogdănici, Diana Elena Săndulache, Corina Andreea Nechita
The aim of the study was to analyze the effects that gadgets have on eyesight quality. A prospective observational study was conducted from January to July 2016, on 60 people who were divided into two groups: Group 1 - 30 middle school pupils with a mean age of 11.9 ± 1.86 and Group 2 - 30 patients evaluated in the Ophthalmology Clinic, "Sf. Spiridon" Hospital, Iași, with a mean age of 21.36 ± 7.16 years. The clinical parameters observed were the following: visual acuity (VA), objective refraction, binocular vision (BV), fusional amplitude (FA), Schirmer's test...
April 2017: Romanian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29449618/codon-based-co-occurrence-network-motifs-in-human-mitochondria
#7
Pramod Shinde, Camellia Sarkar, Sarika Jalan
The nucleotide polymorphism in the human mitochondrial genome (mtDNA) tolled by codon position bias plays an indispensable role in human population dispersion and expansion. Herein, genome-wide nucleotide co-occurrence networks were constructed using data comprised of five different geographical regions and around 3000 samples for each region. We developed a powerful network model to describe complex mitochondrial evolutionary patterns among codon and non-codon positions. We found evidence that the evolution of human mitochondria DNA is dominated by adaptive forces, particularly mutation and selection, which was supported by many previous studies...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29446492/bayesian-reconstruction-of-the-evolutionary-history-and-cross-species-transition-of-variola-virus-and-orthopoxviruses
#8
Gianguglielmo Zehender, Alessia Lai, Carla Veo, Annalisa Bergna, Massimo Ciccozzi, Massimo Galli
Variola virus (VARV), the causative agent of smallpox, is an exclusively human virus belonging to the genus Orthopoxvirus, which includes many other viral species covering a wide range of mammal hosts, such as vaccinia, cowpox, camelpox, taterapox, ectromelia and monkeypox virus. The tempo and mode of evolution of Orthopoxviruses were reconstructed using a Bayesian phylodynamic framework by analysing 80 hemagglutinin sequences retrieved from public databases. Bayesian phylogeography was used to estimate their putative ancestral hosts...
February 15, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29445614/serum-protein-electrophoretic-pattern-in-one-humped-camels-camelus-dromedarius-in-tripoli-libya
#9
Omran Abdoslam, Mahmoud Bayt-Almal, Abdullah Almghrbe, Omran Algriany
The aim of this study was to characterize serum protein capillary electrophoretic pattern in apparently healthy adult male (age: 3-7 years) dromedary camels and also evaluate total protein and albumin levels using automated analyzer. Blood samples were taken from 20 camels. 5ml of blood was collected from the jugular vein and serum was separated from samples by centrifugation. Capillary electrophoresis of serum proteins identified six protein fractions in adult camels, including albumin, alpha1, alpha2, beta1, beta2 and gamma globulins, serum levels of these parameters were 3...
2018: Open Veterinary Journal
https://www.readbyqxmd.com/read/29443069/the-use-of-a-%C3%AE-lactamase-based-conductimetric-biosensor-assay-to-detect-biomolecular-interactions
#10
Marylène Vandevenne, Mathieu Dondelinger, Sami Yunus, Astrid Freischels, Régine Freischels, Oscar Crasson, Noureddine Rhazi, Pierre Bogaerts, Moreno Galleni, Patrice Filée
Biosensors are becoming increasingly important and implemented in various fields such as pathogen detection, molecular diagnosis, environmental monitoring, and food safety control. In this context, we used β-lactamases as efficient reporter enzymes in several protein-protein interaction studies. Furthermore, their ability to accept insertions of peptides or structured proteins/domains strongly encourages the use of these enzymes to generate chimeric proteins. In a recent study, we inserted a single-domain antibody fragment into the Bacillus licheniformis BlaP β-lactamase...
February 1, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29442706/human-adipose-derived-stem-cells-and-osteoblasts-interaction-with-fe-cr-nb-b-magnetic-nanoparticles
#11
Luminita Simion Labusca, Dumitru-Daniel Herea, Ecaterina Radu, Camelia Danceanu, Horia Chiriac, Nicoleta Lupu
The use of materials at nanoscale is currently of increasing interest for life sciences and medicine. Magnetic nanoparticles (MNPs) are under scrutiny for a large array of applications in nanomedicine as diagnostic and therapeutic tools. Proprietary Fe-Cr-Nb-B MNPs display heating properties that recommends them as potent agents for delivery of local hyperthermia for the treatment of solid tumours. Stem cell mediated delivery represents a safe and accurate modality to target remote or metastatic tumour sites...
July 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29440952/antibiotic-treatment-in-childhood-community-acquired-pneumonia-clinical-practice-versus-guidelines-results-from-two-university-hospitals
#12
Sorin Claudiu Man, Valentina Sas, Cristina Schnell, Camelia Florea, Adelina Ţuţu, Ariana Szilágyi, Sergiu Belenes, Amalia Hebriştean, Anca Bonaţ, Claudia Cladovan, Cornel Aldea
Background and aims: Community-acquired pneumonia (CAP) is a both common and serious childhood infection. Antibiotic treatment guidelines help to reduce inadequate antibiotics prescriptions. Methods: We conducted a retrospective study at the Clinical Emergency Hospital for Children, 3rd Pediatric Clinic, Cluj-Napoca and Dr. Gavril Curteanu Clinical City Hospital, in Oradea. All patients discharged with a diagnosis of CAP between December 1, 2014 and February 28, 2015, were included in the study...
2018: Clujul Medical (1957)
https://www.readbyqxmd.com/read/29440485/reporter-nanobody-fusions-ranbodies-as-versatile-small-sensitive-immunohistochemical-reagents
#13
Masahito Yamagata, Joshua R Sanes
Sensitive and specific antibodies are essential for detecting molecules in cells and tissues. However, currently used polyclonal and monoclonal antibodies are often less specific than desired, difficult to produce, and available in limited quantities. A promising recent approach to circumvent these limitations is to employ chemically defined antigen-combining domains called "nanobodies," derived from single-chain camelid antibodies. Here, we used nanobodies to prepare sensitive unimolecular detection reagents by genetically fusing cDNAs encoding nanobodies to enzymatic or antigenic reporters...
February 13, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29438294/first-diagnosed-case-of-camelpox-virus-in-israel
#14
Oran Erster, Sharon Melamed, Nir Paran, Shay Weiss, Yevgeny Khinich, Boris Gelman, Aharon Solomony, Orly Laskar-Levy
An outbreak of a disease in camels with skin lesions was reported in Israel during 2016. To identify the etiological agent of this illness, we employed a multidisciplinary diagnostic approach. Transmission electron microscopy (TEM) analysis of lesion material revealed the presence of an orthopox-like virus, based on its characteristic brick shape. The virus from the skin lesions successfully infected chorioallantoic membranes and induced cytopathic effect in Vero cells, which were subsequently positively stained by an orthopox-specific antibody...
February 13, 2018: Viruses
https://www.readbyqxmd.com/read/29434474/suppression-of-breast-cancer-cell-proliferation-by-selective-single-domain-antibody-for-intracellular-stat3
#15
Sunanda Singh, Genoveva Murillo, Dong Chen, Ashutosh S Parihar, Rajendra G Mehta
Background: The serendipitous discovery of heavy-chain antibodies devoid of light chains in camelids and the subsequent development of VHHs (variable region of camelid heavy chain) have provided a very important tool for research and possibly for therapeutics. In this study, we synthesized single-domain 15-kDa antibody SBT-100 (anti-STAT3 B VHH13) against human STAT3 (signal transducer and activator of transcription) that binds selectively to STAT3 and suppresses the function of phosphorylated STAT3 (p-STAT3)...
2018: Breast Cancer: Basic and Clinical Research
https://www.readbyqxmd.com/read/29434346/yeast-surface-display-platform-for-rapid-discovery-of-conformationally-selective-nanobodies
#16
Conor McMahon, Alexander S Baier, Roberta Pascolutti, Marcin Wegrecki, Sanduo Zheng, Janice X Ong, Sarah C Erlandson, Daniel Hilger, Søren G F Rasmussen, Aaron M Ring, Aashish Manglik, Andrew C Kruse
Camelid single-domain antibody fragments ('nanobodies') provide the remarkable specificity of antibodies within a single 15-kDa immunoglobulin V HH domain. This unique feature has enabled applications ranging from use as biochemical tools to therapeutic agents. Nanobodies have emerged as especially useful tools in protein structural biology, facilitating studies of conformationally dynamic proteins such as G-protein-coupled receptors (GPCRs). Nearly all nanobodies available to date have been obtained by animal immunization, a bottleneck restricting many applications of this technology...
February 12, 2018: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/29433297/preservative-effect-of-camellia-sinensis-l-kuntze-seed-extract-in-soy-sauce-and-its-mutagenicity
#17
Ju Hee Choi, Jin-Yong Kim, Eun Tak Jeong, Tae Ho Choi, Tae Mi Yoon
The purpose of this study was to investigate the applicability of green tea seed (GTS) extract as a natural preservative in food. Food preservative ability and mutagenicity studies of GTS extract and identification of antimicrobial compounds from GTS extract were carried out. The GTS extract showed only anti-yeast activity against Candida albicans with MIC value of 938μg/mL and Zygosaccharomyces rouxii with a MIC of 469μg/mL. The active compounds were identified as theasaponin E1 (1), assamsaponin A (2), and assamsaponin B (3)...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29433051/exploring-optimal-supplement-strategy-of-medicinal-herbs-and-tea-extracts-for-bioelectricity-generation-in-microbial-fuel-cells
#18
Bor-Yann Chen, Jia-Hui Liao, An-Wei Hsu, Po-Wei Tsai, Chung-Chuan Hsueh
This first-attempt study used extracts of appropriate antioxidant abundant Camellia and non-Camellia tea and medicinal herbs as model ESs to stably intensify bioelectricity generation performance in microbial fuel cells (MFCs). As electron shuttles (ESs) could stimulate electron transport phenomena by significant reduction of electron transfer resistance, the efficiency of power generation for energy extraction in microbial fuel cells (MFCs) could be appreciably augmented. Using environmentally friendly natural bioresource as green bioresource of ESs is the most promising to sustainable practicability...
February 2, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29432034/cognitive-functioning-in-chronic-post-stroke-aphasia
#19
José Fonseca, Ana Raposo, Isabel Pavão Martins
There is a minimal amount of knowledge regarding the cognitive abilities of people with aphasia. We evaluated the performance of individuals with chronic aphasia (AP) and control participants without aphasia (CP) with left hemisphere stroke in a battery of nonverbal cognitive tests and its relationship with aphasia severity, comprehension abilities, and speech fluency in a prospective cross-sectional study. Cognitive evaluation comprised 10 nonverbal tests. Scores were converted to age and education adjusted standard scores...
February 12, 2018: Applied Neuropsychology. Adult
https://www.readbyqxmd.com/read/29429331/investigation-of-lipid-metabolism-by-a-new-structured-lipid-with-medium-and-long-chain-triacylglycerols-from-cinnamomum-camphora-seed-oil-in-healthy-c57bl-6j-mice
#20
Jiang-Ning Hu, Jin-Rong Shen, Chao-Yue Xiong, Xue-Mei Zhu, Ze-Yuan Deng
In the present study, a new structured lipid with medium- and long-chain triacylglycerols (MLCTs) was synthesized from camellia oil (CO) and Cinnamomum camphora seed oil (CCSO) by enzymatic interesterification. Meanwhile, the antiobesity effects of structured lipid were investigated through observing the changes of enzymes related to lipid mobilization in healthy C57BL/6J mice. Results showed that after synthesis, the major triacylgeride (TAG) species of intesterificated product changed to LaCC/CLaC (12.6 ± 0...
February 14, 2018: Journal of Agricultural and Food Chemistry
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