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Q Wen, K Gandhi, Rebecca A Capel, G Hao, C O'Shea, G Neagu, S Pearcey, D Pavlovic, Derek A Terrar, J Wu, G Faggian, Patrizia Camelliti, M Lei
Transmural and regional gradients in membrane potential and Ca2+ transient in the murine heart are largely unexplored. Here, we developed and validated a robust approach which combines transverse ultra-thin cardiac slices and high resolution optical mapping to enable systematic analysis of transmural and regional gradients in transmembrane potential (Vm) and intracellular Ca2+ transient (CaT) across the entire murine ventricles. The voltage dye RH237 or Ca2+ dye Rhod-2 AM were loaded through the coronary circulation using a Langendorff perfusion system...
June 21, 2018: Journal of Physiology
Thibaud Kuca, Thomas Passler, Benjamin W Newcomer, John D Neill, Patricia K Galik, Kay P Riddell, Yijing Zhang, Paul H Walz
Bovine viral diarrhea virus (BVDV) is an economically important pathogen of cattle that can also infect a wide range of domestic and wild species including sheep, goats, deer, camelids, and pigs. BVDV isolates are genetically highly diverse and previous work demonstrated that many substitutions were introduced in the viral genome during acute infections in cattle. In contrast, only limited information exists regarding changes occurring during BVDV infections in species other than cattle. The purpose of this study was to determine the changes introduced in the open reading frame (ORF) of the BVDV genome during serial infection of pregnant cattle and sheep with an isolate of bovine origin...
2018: Frontiers in Microbiology
Menha Swellam, Rasha F K Zahran, Hossam Abo El-Sadat Taha, Nashwa El-Khazragy, Camelia Abdel-Malak
Aberrant expression of miRNAs has a link with tumorgenesis and their deregulation is reported in biological fluids of cancer patients. Authors aimed to investigate the diagnostic role of miRNA-17-5p, miR-155 and miRNA-222 in serum samples from breast cancer patients (n = 80), benign breast patients (n = 40) and healthy individuals (n = 30) using quantitative real-time PCR technique. Median levels of investigated markers revealed significant increase in primary breast cancer followed by benign and control groups...
June 20, 2018: Archives of Physiology and Biochemistry
Seyed Reza Banihashemi, Ahmad Zavaran Hosseini, Fatemeh Rahbarizadeh, Davoud Ahmadvand
Objectives: CD19 is a transmembrane glycoprotein of immunoglobulin superfamily. In order to treat lymphoma, monoclonal antibodies (mAb) can target different antigens, including CD19, CD20 and CD22 on the surface of B-cells. Along with biotechnology progress, a new generation of antibodies is introduced, with the purpose of eliminating the defects of the previous generation. Among the most developed one are nanobodies (Nb). Nbs are a unique kind of camelid single domain antibody fragments with a broad range of medical applications...
May 2018: Iranian Journal of Basic Medical Sciences
Kevin A Henry, C Roger MacKenzie
Single-domain antibodies (sdAbs), the autonomous variable domains of heavy chain-only antibodies produced naturally by camelid ungulates and cartilaginous fishes, have evolved to bind antigen using only three complementarity-determining region (CDR) loops rather than the six present in conventional VH :VL antibodies. It has been suggested, based on limited evidence, that sdAbs may adopt paratope structures that predispose them to preferential recognition of recessed protein epitopes, but poor or non-recognition of protuberant epitopes and small molecules...
June 19, 2018: MAbs
Xinlong Dai, Guifu Zhao, Tianming Jiao, Yingling Wu, Xinmin Li, Kan Zhou, Liping Gao, Tao Xia
UDP-rhamnose synthase (RHM), the branch-point enzyme controlling the nucleotide sugar interconversion pathway, converts UDP-D-glucose into UDP-rhamnose. As a rhamnose residue donor, UDP-L-rhamnose is essential for the biosynthesis of pectic polysaccharides and secondary metabolites in plants. In this study, three CsRHM genes from tea plants (Camellia sinensis) were cloned and characterized. Enzyme assays showed that three recombinant proteins displayed RHM activity and were involved in the biosynthesis of UDP-rhamnose in vitro...
June 19, 2018: Journal of Agricultural and Food Chemistry
G Camelia Adams, Andrew J Wrath, Xiangfei Meng
OBJECTIVE: Several studies have shown a relationship between individual attachment and various aspects of treatment utilization in individuals with medical problems as well as mental health disorders. This review systematically evaluates existing literature targeting the relationship between attachment and all aspects of treatment utilization, such as engagement, participation, and completion, in adults with mental health problems. METHOD: A computerized search of PsycINFO, Medline, Embase, PubMed, and Healthstar and a manual search were employed...
January 1, 2018: Canadian Journal of Psychiatry. Revue Canadienne de Psychiatrie
Shihua Zhang, Liang Zhang, Yuling Tai, Xuewen Wang, Chi-Tang Ho, Xiaochun Wan
Characteristic secondary metabolites, including flavonoids, theanine and caffeine, in the tea plant ( Camellia sinensis ) are the primary sources of the rich flavors, fresh taste, and health benefits of tea. The decoding of genes involved in these characteristic components is still significantly lagging, which lays an obstacle for applied genetic improvement and metabolic engineering. With the popularity of high-throughout transcriptomics and metabolomics, 'omics'-based network approaches, such as gene co-expression network and gene-to-metabolite network, have emerged as powerful tools for gene discovery of plant-specialized (secondary) metabolism...
2018: Frontiers in Plant Science
Dominik P Buser, Kai D Schleicher, Cristina Prescianotto-Baschong, Martin Spiess
Retrograde transport of membranes and proteins from the cell surface to the Golgi and beyond is essential to maintain homeostasis, compartment identity, and physiological functions. To study retrograde traffic biochemically, by live-cell imaging or by electron microscopy, we engineered functionalized anti-GFP nanobodies (camelid VHH antibody domains) to be bacterially expressed and purified. Tyrosine sulfation consensus sequences were fused to the nanobody for biochemical detection of trans -Golgi arrival, fluorophores for fluorescence microscopy and live imaging, and APEX2 (ascorbate peroxidase 2) for electron microscopy and compartment ablation...
June 18, 2018: Proceedings of the National Academy of Sciences of the United States of America
Yumei Qian, Shuxiang Zhang, Shengbo Yao, Jinxin Xia, Yanzhi Li, Xinlong Dai, Wenzhao Wang, Xiaolan Jiang, Yajun Liu, Mingzhuo Li, Liping Gao, Tao Xia
BACKGROUND: Tea plants [Camellia sinensis (L.) O. Kuntze] can produce one of the three most widely popular non-alcoholic beverages throughout the world. Polyphenols and volatiles are the main functional ingredients determining tea's quality and flavor; however, the biotic or abiotic factors affecting tea polyphenol biosynthesis are unclear. This paper focuses on the molecular mechanisms of sucrose on polyphenol biosynthesis and volatile composition variation in tea plants. RESULTS: Metabolic analysis showed that the total content of anthocyanins, catechins, and proanthocyanidins(PAs) increased with sucrose, and they accumulated most significantly after 14 days of treatment...
June 18, 2018: BMC Plant Biology
Ling-Yan Jia, Hui-Long Xia, Zhi-Da Chen, Casey Compton, Heather Bucur, Devendra A Sawant, Gary O Rankin, Bo Li, You-Ying Tu, Yi Charlie Chen
Ovarian cancer has the highest mortality rate of all gynecological malignancies and the five-year death rate of patients has remained high in the past five decades. Recently, with the rise of cancer stem cells (CSCs) theory, an increasing amount of research has suggested that CSCs give rise to tumor recurrence and metastasis. Theasaponin E₁ (TSE₁), which was isolated from green tea ( Camellia sinensis ) seeds, has been proposed to be an effective compound for tumor treatment. However, studies on whether TSE₁ takes effect through CSCs have rarely been reported...
June 17, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Dan David, Ditza Rotenberg, Evgeny Khinich, Oran Erster, Svetlana Bardenstein, Michael van Straten, Nisreen M A Okba, Stalin V Raj, Bart L Haagmans, Marcelo Miculitzki, Irit Davidson
Thus far, no human MERS-CoV infections have been reported from Israel. Evidence for the circulation of MERS-CoV in dromedaries has been reported from almost all the countries of the Middle East, except Israel. Therefore, we aimed to analyze MERS-CoV infection in Israeli camelids, sampled between 2012 and 2017. A total of 411 camels, 102 alpacas and 19 llamas' sera were tested for the presence of antibodies to MERS-CoV. Our findings indicate a lower MERS-CoV seropositivity among Israeli dromedaries than in the surrounding countries, and for the first time naturally infected llamas were identified...
June 2018: One Health
Nisar A Wani, Seungbum Hong, Binoy S Vettical
Studies were conducted to evaluate the adequate time for exposure of donor nucleus to oocyte cytoplast before its activation and the effect of oocyte source on the development of SCNT embryos in camels. A higher number of embryos cleaved and developed to blastocyst stage (P < 0.05) when couplets were activated between 1 and 2 h-than that of those activated at 0.5 h or more than 2 h post-fusion. A reduced number of reconstructed embryos cleaved (55.2 ± 7.6%) and developed to the blastocyst stage (20...
May 25, 2018: Theriogenology
Mehmet E Ozseyhan, Pengcheng Li, GunNam Na, Zhenjing Li, Changlu Wang, Chaofu Lu
The fatty acid profile of plant oils determines their quality and uses. Saturated fatty acids are often not desirable from the standpoints of nutrition and some industrial applications. Camelina sativa is a re-emerged oilseed crop, however its oil needs to be improved to meet different application requirements. In this study, saturated fatty acids were greatly reduced by down-regulating genes encoding the fatty acyl-ACP thioesterases (FATB). An artificial microRNA (amiFATB) was created by replacing a microRNA sequence in the camelina Csa-miR159a gene with a FATB gene specific sequence...
June 12, 2018: Biochemical and Biophysical Research Communications
Camellia Torabizadeh, Tayebeh Bahmani, Zahra Molazem, Seyed Alireza Moayedi
Effective communications play a significant role in environments where health care is provided. A review of the available literature did not yield a valid scale for evaluation of operating room nurse-doctor communication. Accordingly, the present study is an attempt at development and psychometric evaluation of a professional communication questionnaire for the operating room. The present study is a methodological work conducted in two steps. In the first stage of the study, 56 items on a 5-point Likert scale were developed according to the results of a review of relevant literature and several meetings with experts...
June 14, 2018: Health Communication
Eva Maria Greunz, Anne K H Krogh, Wouter Pieters, Olga Amann Ruiz, Julia Bohner, Anja Reckendorf, Davide Monaco, Mads Frost Bertelsen
  Acute-phase reactants indicate inflammation and are increasingly used in veterinary medicine to indicate and to monitor progression of disease. Hemostasis and inflammation have interconnected pathophysiologic pathways and influence each other on different levels. This study established observed normal ranges for acute-phase reactants and for coagulation and thromboelastographic (TEG) parameters in 49 dromedary camels ( Camelus dromedarius) and assessed the response to chronic and acute inflammation. Chronically infected animals suffering from lymph abscessation due to Corynebacterium spp...
June 2018: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Laura Endres, Diana Uivaroşan, Delia Mirela Ţiţ, Ovidiu Pop, Simona Bungău, Camelia Buhaş
No abstract text is available yet for this article.
April 2018: Iranian Journal of Public Health
Banafsheh Tehranineshat, Mahnaz Rakhshan, Camellia Torabizadeh, Mohammad Fararouei
BACKGROUND: Compassion is the core of nursing care and the basis of ethical codes. Due to the complex and abstract nature of this concept, there is a need for further investigations to explore the meaning and identify compassionate nursing care. OBJECTIVES: The purpose of this study was to identify and describe compassionate nursing care based on the experiences of nurses, patients, and family caregivers. RESEARCH DESIGN: This was a qualitative exploratory study...
January 1, 2018: Nursing Ethics
Mohamed Boumaiza, Haifa Chahed, Aymen Ezzine, Maryse Jaouen, Alessandra Gianoncelli, Giovanna Longhi, Fernando Carmona, Paolo Arosio, Marie-Agnès Sari, Mohamed Nejib Marzouki
No abstract text is available yet for this article.
July 2018: Journal of Molecular Recognition: JMR
Wei-Ting Lee, Yu-Tang Tung, Chun-Ching Wu, Pang-Shuo Tu, Gow-Chin Yen
Ulcerative colitis (UC), one type of chronic inflammatory bowel disease (IBD), is a chronic and recurrent disorder of the gastrointestinal (GI) tract. As camellia oil (CO) is traditionally used to treat GI disorders, this study investigated the role of CO on acetic acid-induced colitis in the rat. The composition of the gut microbial community is related to many diseases, thus, this study also investigated the effects of CO on the composition of the gut microbiota. The rats were fed a dose of 2 mL/kg body weight CO, olive oil (OO), or soybean oil (SO) once a day for 20 days, and the gut microbiota was analyzed using 16S rRNA gene sequencing...
June 13, 2018: Journal of Agricultural and Food Chemistry
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