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https://www.readbyqxmd.com/read/27992487/changes-in-host-cytokine-patterns-of-tb-patients-with-different-bacterial-loads-detected-using-16s-rrna-analysis
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Rhiannon Heslop, Adama L Bojang, Sheikh Jarju, Joseph Mendy, Sarah Mulwa, Ousman Secka, Francis S Mendy, Olumuyiwa Owolabi, Beate Kampmann, Jayne S Sutherland
BACKGROUND: Tuberculosis (TB) has overtaken HIV as the biggest infectious disease killer, with the majority of deaths occurring in sub-Saharan Africa. However it is unknown how differences in bacterial load alter host immune profiles in the sputum and blood of TB patients. METHODS: 16S ribosomal RNA analysis was used to determine bacterial load in sputum samples obtained from 173 patients with active TB (57 pre-treatment and 116 post-treatment). Host analyte concentrations in sputum and Mycobacterium tuberculosis (Mtb) antigen stimulated whole blood assay supernatants were analysed using multiplex cytokine arrays...
2016: PloS One
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