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Umbilical cord Mesenchymal stem cells

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https://www.readbyqxmd.com/read/29035685/mechanistic-effect-of-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-on-the-submandibular-salivary-gland-in-ovariectomized-rats
#1
Zienab Gouda, Mohamed Khalifa, Sally Shalaby, Samia Hussein
To reveal the effect of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on the submandibular gland after bilateral ovariectomy, 21 adult Sprague-Dawley female rats were divided equally into three groups: sham-operated group (SHAM), ovariectomized group (OVX) and OVX received repeated intravenous injection of hUCB-MSCs group (OVX + hUCB-MSCs). RT-PCR for AQP3, AQP4, AQP5 and BMP6 gene expression was determined. The cellular localization and expression of anti-human CD105 anti-human CD34, anti-proliferating nuclear antigen (PCNA), anti-single stranded DNA(ssDNA), anti-Caspase 3, anti-aquaporin 1(AQP1) and anti-α smooth muscle actin(α-SMA) were determined immunohistochemically...
October 16, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29035086/notch-signaling-is-essential-for-maturation-self-renewal-and-tri-differentiation-of-in-vitro-derived-human-neural-stem-cells
#2
Katari Venkatesh, L Vinod Kumar Reddy, Salar Abbas, Madhubanti Mullick, Erfath Thanjeem Begum Moghal, Janardhana Papayya Balakrishna, Dwaipayan Sen
Although neural stem cells (NSCs) have potential applications in treating neurological disorders, much still needs to be understood about the differentiation biology for their successful clinical translation. In this study, we aimed at deriving NSCs from human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) and explored the role of Notch signaling in the differentiation process. The hUCB-MSCs were characterized as per guidelines of the International Society of Cellular Therapy. NSCs were successfully generated from hUCB-MSCs by using epidermal and fibroblast growth factors under serum-free conditions...
October 16, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/29023156/stem-cell-therapy-for-articular-cartilage-repair-review-of-the-entity-of-cell-populations-used-and-the-result-of-the-clinical-application-of-each-entity
#3
Yong-Beom Park, Chul-Won Ha, Ji Heon Rhim, Han-Jun Lee
BACKGROUND: Following successful preclinical studies, stem cell therapy is emerging as a candidate for the treatment of articular cartilage lesions. Because stem cell therapy for cartilage repair in humans is at an early phase, confusion and errors are found in the literature regarding use of the term stem cell therapy in this field. PURPOSE: To provide an overview of the outcomes of cartilage repair, elucidating the various cell populations used, and thus reduce confusion with regard to using the term stem cell therapy...
October 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29019171/optimization-of-culture-conditions-for-rapid-clinical-scale-expansion-of-human-umbilical-cord-blood-derived-mesenchymal-stem-cells
#4
Wankyu Choi, Soon-Jae Kwon, Hye Jin Jin, Sang Young Jeong, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Hong Bae Jeon, Eun Su Jeon
BACKGROUND: Mesenchymal stem cells (MSCs) have broad-spectrum therapeutic effects in various diseases, and thus have many clinical applications. However, it is difficult to produce sufficient numbers of MSCs for clinical use, and improved culture systems are required. Here, we report the effects of calcium (Ca(2+)) and hypoxia on the proliferation of human umbilical cord blood-derived MSCs (hUCB-MSCs). In addition, we determined the optimal conditions of these two factors for the large-scale culture of hUCB-MSCs...
October 10, 2017: Clinical and Translational Medicine
https://www.readbyqxmd.com/read/29017529/short-review-on-human-umbilical-cord-lining-epithelial-cells-and-their-potential-clinical-applications
#5
REVIEW
Razwa Saleh, Hasan Mahmud Reza
BACKGROUND: The human umbilical cord has been studied extensively in the past two decades. It is free of ethical dilemmas, non-tumorigenic, and less immunogenic and thus provides a significant advantage over other stem cell sources. The cord lining yields both mesenchymal and epithelial stem cells. The mesenchymal cells have been appraised at length by many researchers, which led to the current review focusing on the cord lining epithelial cells (CLECs). These cells have high proliferative capacity and their superior harvest and multiplication, using the revolutionary CellOptima(TM) technology, makes them better candidates in comparison to contemporary adult stem cells...
October 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29016394/mir-20b-106a-modulate-ngn2-gene-expression-during-neural-differentiation-of-human-umbilical-cord-mesenchymal-stem-cells
#6
Haoyu Wang, Wenrui Ban, Tao Wang, Zhuo Li, Xiaoqian Dang
The aim of this study was to perform neural differentiation of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord (HUMSCs) and explore the role of miR-20b and miR-106a, which may regulate Neurogenin-2 (Ngn2) expression during the neural differentiation. HUMSCs were cultured and induced in vitro. The cells were stained for nestin and microtubule-associated protein 2 (MAP2) by immunofluorescence. The interactional binding sites between the 3'-untranslated region (3'-UTR) of Ngn2 mRNA and miR-20b or miR-106a were predicted by bioinformatics and identified by a dual-luciferase assay...
October 9, 2017: Neuroreport
https://www.readbyqxmd.com/read/28992805/the-effects-of-liquid-crystal-based-composite-substrates-on-cell-functional-responses-of-human-umbilical-cord-derived-mesenchymal-stem-cells-by-mechano-regulatory-process
#7
Hao Wu, Yupan Shang, Jiaqing Zhang, Lek Hang Cheang, Xiaoli Zeng, Mei Tu
Physical properties of extracellular matrix, including elasticity and microstructure, have been considered as important factors inducing stem cell differentiation. This study developed a novel type of liquid crystal-based matrix by combining the elastic property of polyurethane with viscoelastic liquid crystal to generate a soft elastic response resembling physical microenvironment of stem cell niche, and explored the mechano-driving cell behaviors. Addition of varying liquid crystal concentration (10 wt%, 30 wt% and 50 wt%) had great effects on surface morphology and elastic modulus of liquid crystal/ polyurethane composite substrates...
October 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28978275/low-immunogenic-endothelial-cells-maintain-morphofunctional-properties-needed-for-tissue-engineering
#8
Skadi Lau, Dorothee Eicke, Marco Carvalho Oliveira, Bettina Wiegmann, Claudia Schrimpf, Axel Haverich, Rainer Blasczyk, Mathias Wilhelmi, Constanςa Figueiredo, Ulrike Böer
The limited availability of native vessels suitable for the application as hemodialysis shunts or bypass material demands new strategies in cardiovascular surgery. Tissue engineered vascular grafts containing autologous cells are considered as ideal vessel replacements due to the low risk of rejection. However, endothelial cells (EC), which are central components of natural blood vessels, are difficult to obtain from elderly patients of poor health. Umbilical cord blood represents a promising alternative source for EC but their allogeneic origin corresponds with the risk of rejection after allotransplantation...
October 5, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28975647/effect-of-metformin-and-celecoxib-on-cytotoxicity-and-release-of-gdf-15-from-human-mesenchymal-stem-cells-in-high-glucose-condition
#9
Elaheh Zafarvahedian, Azam Roohi, Mohammad Reza Sepand, Seyed Nasser Ostad, Mohammad Hossein Ghahremani
Mesenchymal stem cells (MSCs) have therapeutic potential for treatment of diabetes. However, in vitro behavior of MSCs in high glucose condition as well as presence of glucose lowering agents is not fully understood. Because MSCs have an important role in tissue repair, we examined the effects of metformin and celecoxib on viability of MSCs in different glucose conditions. MSCs, from umbilical cord blood, were cultured in normoglycemic (glucose 5.5 mM), midglycemic (glucose 10 mM), and hyperglycemic (glucose 25 mM) conditions, and the cell viability was evaluated by MTT assay...
October 4, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28975370/mesenchymal-stem-cells-from-preterm-to-term-newborns-undergo-a-significant-switch-from-anaerobic-glycolysis-to-the-oxidative-phosphorylation
#10
Silvia Ravera, Marina Podestà, Federica Sabatini, Chiara Fresia, Marta Columbaro, Silvia Bruno, Ezio Fulcheri, Luca Antonio Ramenghi, Francesco Frassoni
We evaluated the energy metabolism of human mesenchymal stem cells (MSC) isolated from umbilical cord (UC) of preterm (< 37 weeks of gestational age) and term (≥ 37 weeks of gestational age) newborns, using MSC from adult bone marrow as control. A metabolic switch has been observed around the 34th week of gestational age from a prevalently anaerobic glycolysis to the oxidative phosphorylation. This metabolic change is associated with the organization of mitochondria reticulum: preterm MSCs presented a scarcely organized mitochondrial reticulum and low expression of proteins involved in the mitochondrial fission/fusion, compared to term MSCs...
October 3, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28974553/safety-and-efficacy-of-the-intravenous-infusion-of-umbilical-cord-mesenchymal-stem-cells-in-patients-with-heart-failure-a-phase-1-2-randomized-controlled-trial-rimecard-trial
#11
Jorge G Bartolucci, Fernando J Verdugo, Paz L González, Ricardo E Larrea, Ema Abarzua, Carlos Goset, Pamela G Rojo, Ivan Palma, Ruben Lamich, Pablo A Pedreros, Gloria Valdivia, Valentina M Lopez, Carolina Nazzal, Francisca Alcayaga, Jimena Cuenca, Matthew J Brobeck, Amit N Patel, Fernando E Figueroa, Maroun Khoury
Rationale: Umbilical cord-derived mesenchymal stem cells (UC-MSC) are easily accessible and expanded in vitro, possess distinct properties, and improve myocardial remodeling and function in experimental models of cardiovascular disease. While bone marrow-derived mesenchymal stem cells (BM-MSCs) have been previously assessed for their therapeutic potential in individuals with heart failure and reduced ejection fraction (HFrEF), no clinical trial has evaluated UC-MSCs in these patients. Objective: Evaluate the safety and efficacy of the infusion of UC-MSC in patients with chronic stable HFrEF...
September 26, 2017: Circulation Research
https://www.readbyqxmd.com/read/28969635/impact-of-mesenchymal-stem-cells-secretome-on-glioblastoma-pathophysiology
#12
Joana Vieira de Castro, Eduardo D Gomes, Sara Granja, Sandra I Anjo, Fátima Baltazar, Bruno Manadas, António J Salgado, Bruno M Costa
BACKGROUND: Glioblastoma (GBM) is a highly aggressive primary brain cancer, for which curative therapies are not available. An emerging therapeutic approach suggested to have potential to target malignant gliomas has been based on the use of multipotent mesenchymal stem cells (MSCs), either unmodified or engineered to deliver anticancer therapeutic agents, as these cells present an intrinsic capacity to migrate towards malignant tumors. Nevertheless, it is still controversial whether this innate tropism of MSCs towards the tumor area is associated with cancer promotion or suppression...
October 2, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28967542/current-evidence-of-adult-stem-cells-to-enhance-anterior-cruciate-ligament-treatment-a-systematic-review-of-animal-trials
#13
REVIEW
Ruipeng Guo, Liang Gao, Bin Xu
PURPOSE: To systematically review the available preclinical evidence of adult stem cells as a biological augmentation in the treatment of animal anterior cruciate ligament (ACL) injury. STUDY DESIGN: Systematic review. METHODS: PubMed (MEDLINE) and Embase were searched for the eligible studies. The inclusion criteria were controlled animal trials of adult stem cells used in ACL treatment (repair or reconstruction). Studies of natural ACL healing without intervention, in vitro studies, ex vivo studies, and studies without controls were excluded...
September 26, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28964742/umbilical-cord-mesenchymal-stromal-cell-transplantations-a-systemic-analysis-of-clinical-trials
#14
REVIEW
Alp Can, Ferda Topal Celikkan, Ozgur Cinar
The advances and success of umbilical cord-derived mesenchymal stromal cells (UC-MSCs) in experimental disease animal models have fueled the development of targeted therapies in humans. The therapeutic potential of allogeneic transplantation of UC-MSCs has been under examination since 2009. The purpose of this systematic analysis was to review the published results, limitations and obstacles for UC-MSC transplantation. An extensive search strategy was applied to the published literature, 93 peer-reviewed full-text articles and abstracts were found published by early August 2017 that investigated the safety, efficacy and feasibility of UC-MSCs in 2001 patients with 53 distinct pathologies including many systemic/local, acute/chronic conditions...
September 28, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28963712/3d-decellularized-native-extracellular-matrix-scaffold-for-in-vitro-culture-expansion-of-human-wharton-s-jelly-derived-mesenchymal-stem-cells-hwj-mscs
#15
Balasubramanian Sundaram, Anne George Cherian, Sanjay Kumar
Mesenchymal stem cells (MSCs) are derived from Wharton's jelly tissue of the human umbilical cord. Given appropriate culture conditions, these cells can self-renew and differentiate into multiple cell types across the lineages. Among the properties exhibited by these cells, immunomodulation through secretion of trophic factors has been widely exploited in a broad spectrum of preclinical/clinical regenerative applications. Moreover, the extracellular matrix is found to play a major role apart from niche cells in determining stem cell fate including that of MSCs...
September 26, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28962669/sonic-hedgehog-shh-signaling-improves-the-angiogenic-potential-of-wharton-s-jelly-derived-mesenchymal-stem-cells%C3%A2-wj-msc
#16
Gabriela Zavala, Catalina P Prieto, Andrea A Villanueva, Verónica Palma
BACKGROUND: Wharton's jelly-derived mesenchymal stem cells (WJ-MSC) show remarkable therapeutic potential to repair tissue upon injury via paracrine signaling by secreting diverse trophic factors that promote angiogenesis. However, the mechanisms and signaling pathways that regulate the induction of these specific factors are still mostly unknown. Emerging evidence suggests that Sonic hedgehog (SHH) plays a central role in angiogenesis and tissue maintenance. However, its contribution to the angiogenic potential of MSC has not been fully addressed...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28962646/pil6-trail-engineered-umbilical-cord-mesenchymal-stromal-stem-cells-are-highly-cytotoxic-for-myeloma-cells-both-in-vitro-and-in-vivo
#17
Paola Cafforio, Luigi Viggiano, Francesco Mannavola, Eleonora Pellè, Concetta Caporusso, Eugenio Maiorano, Claudia Felici, Francesco Silvestris
BACKGROUND: Mesenchymal/stromal stem cells (MSCs) are favorably regarded in anti-cancer cytotherapies for their spontaneous chemotaxis toward inflammatory and tumor environments associated with an intrinsic cytotoxicity against tumor cells. Placenta-derived or TRAIL-engineered adipose MSCs have been shown to exert anti-tumor activity in both in-vitro and in-vivo models of multiple myeloma (MM) while TRAIL-transduced umbilical cord (UC)-MSCs appear efficient inducers of apoptosis in a few solid tumors...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28962642/upregulation-of-sodium-taurocholate-cotransporter-polypeptide-during-hepatogenic-differentiation-of-umbilical-cord-matrix-mesenchymal-stem-cells-facilitates-hepatitis-b-entry
#18
Camillo Sargiacomo, Hoda El-Kehdy, Kai Dallmeier, Joery de Kock, Clara Hernandez-Kelly, Vera Rogiers, Arturo Ortega, Johan Neyts, Etienne Sokal, Mustapha Najimi
BACKGROUND: Hepatitis B virus (HBV) carriers worldwide number approximately 240 million people and around 780,000 people die every year from HBV infection. HBV entry and uptake are functionally linked to the presence of the human sodium-taurocholate cotransporting peptide (hNTCP) receptor. Recently, our group demonstrated that human umbilical cord matrix stem cells (UCMSCs) become susceptible to HBV after in-vitro hepatogenic differentiation (D-UCMSCs). METHODS: In the present study, we examined the involvement of hNTCP in governing D-UCMSC susceptibility to HBV infection by characterizing the modulation of this transporter expression during hepatogenic differentiation and by appreciating the inhibition of its activity on infection efficacy...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28960800/in-vitro-cartilage-construct-generation-from-silk-fibroin-chitosan-porous-scaffold-and-umbilical-cord-blood-derived-human-mesenchymal-stem-cells-in-dynamic-culture-condition
#19
Parinita Agrawal, Krishna Pramanik, Amit Biswas, Ranjan Ku Patra
Cartilage construct generation includes a scaffold with appropriate composition to mimic matrix of the damaged tissue on which the stem cells grow and differentiate. In this study, umbilical cord blood (UCB) derived human mesenchymal stem cells (hMSCs) were seeded on freeze dried porous silk-fibroin (SF)/chitosan (CS) scaffolds. Influence of static and dynamic (spinner flask bioreactor) culture conditions on the developing cartilage construct were studied by in-vitro characterization for viability, proliferation, distribution and chondrogenic differentiation of hMSCs over the scaffold...
September 29, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28958480/protein-profiling-and-angiogenic-effect-of-hypoxia-cultured-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-in-hindlimb-ischemia
#20
Kyu-Hyun Han, Ae-Kyeong Kim, Min-Hee Kim, Do-Hyung Kim, Ha-Nl Go, Donglim Kang, Jong Wook Chang, Soon Won Choi, Kyung-Sun Kang, Dong-Ik Kim
The aim of the present study was to investigate protein profiles of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) cultured in normoxic (21% O2) and hypoxic (1% O2) conditions, and evaluate oxygenation effects on angiogenesis in an ischemic hindlimb mouse model using a modified ischemic scoring system. Hypoxic conditions did not change the expression of phenotypic markers and increased adipogenesis and chondrogenesis. Epidermal growth factor (EGF), transforming growth factor alpha (TGF-α), TGF-β RII, and vascular endothelial growth factor (VEGF) were upregulated in the conditioned medium of hypoxic hUCB-MSCs, which are commonly related to angiogenesis and proliferation of biological processes by Gene Ontology...
September 20, 2017: Tissue & Cell
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