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Umbilical cord Mesenchymal stem cells

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https://www.readbyqxmd.com/read/27909741/the-role-of-epigenetic-modifiers-in-extended-cultures-of-functional-hepatocyte-like-cells-derived-from-human-neonatal-mesenchymal-stem-cells
#1
M Cipriano, J C Correia, S P Camões, N G Oliveira, P Cruz, H Cruz, M Castro, J L Ruas, J M Santos, J P Miranda
The development of predictive in vitro stem cell-derived hepatic models for toxicological drug screening is an increasingly important topic. Herein, umbilical cord tissue-derived mesenchymal stem cells (hnMSCs) underwent hepatic differentiation using an optimized three-step core protocol of 24 days that mimicked liver embryogenesis with further exposure to epigenetic markers, namely the histone deacetylase inhibitor trichostatin A (TSA), the cytidine analogue 5-azacytidine (5-AZA) and dimethyl sulfoxide (DMSO)...
December 1, 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27904497/preparation-of-polypyrrole-embedded-electrospun-poly-lactic-acid-nanofibrous-scaffolds-for-nerve-tissue-engineering
#2
Jun-Feng Zhou, Yi-Guo Wang, Liang Cheng, Zhao Wu, Xiao-Dan Sun, Jiang Peng
Polypyrrole (PPy) is a biocompatible polymer with good conductivity. Studies combining PPy with electrospinning have been reported; however, the associated decrease in PPy conductivity has not yet been resolved. We embedded PPy into poly(lactic acid) (PLA) nanofibers via electrospinning and fabricated a PLA/PPy nanofibrous scaffold containing 15% PPy with sustained conductivity and aligned topography. There was good biocompatibility between the scaffold and human umbilical cord mesenchymal stem cells as well as Schwann cells...
October 2016: Neural Regeneration Research
https://www.readbyqxmd.com/read/27899238/goat-umbilical-cord-cells-are-permissive-to-small-ruminant-lentivirus-infection-in-vitro
#3
Gabrielle R Martins, Rebeca C Marinho, Rosivaldo Q Bezerra Junior, Antoniel de O Alves, Lilia M C Câmara, Luiz C Albuquerque-Pinto, Maria F da S Teixeira
Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture...
November 19, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27894407/mirna-378-controls-cell-proliferation-in-rabbit-umbilical-cord-mesenymal-stem-cells
#4
H Wang, Z Zhao, J Du, T Wang, L Yang, H Yu
Mesenchymal stem cells (MSC) are known to have the ability to differentiate into various lineages of mesenchymal tissue. They are widely distributed in a variety of tissues in the body and are also present in the foetal environment. MicroRNAs (miRNAs) are a fundamental class of biological molecules that play a crucial role in development. In this study, microRNA-378 and its predicted target (Sufu, suppressor of fused homolog) were used to study proliferation of rabbit umbilical cord mesenymal stem cells, luciferase reporter assay was used to assess and confirm the binding sequence of 3'untranslated region between microRNA-378 and Sufu...
October 31, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27892545/erythrocyte-membrane-based-cationic-polymer-mcdna-complexes-as-an-efficient-gene-delivery-system
#5
Ping Huang, Jing Zhao, Chiju Wei, Xiaohu Hou, Pingzhang Chen, Yan Tan, Cheng-Yi He, Zhiyong Wang, Zhi-Ying Chen
Gene therapy has great promise for the treatment of obtained and inherited serious diseases. However, the lack of safe and efficient gene delivery systems remains a barrier for their clinical application. Here, we reported a potential gene delivery vehicle composed of the erythrocyte membrane and cationic polymers, for example the XtremeGENE from Roche and the ε-caprolactone modified polyethylenimine. In addition to high efficiency, this system showed negligible cytotoxicity compared to the two cationic polymers alone in various cell lines, including human embryonic kidney cells (293T), human liver cancer cells (Huh7 and HepG2), murine dendritic cells (DC2...
November 28, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27888544/conditioned-medium-a-new-alternative-for-cryopreservation-of-equine-umbilical-cord-mesenchymal-stem-cells
#6
Leandro Maia, Marianne Camargos Dias, Carolina Nogueira de Moraes, Camila de Paula Freitas-Dell'aqua, Ligia S L Silveira da Mota, Valquíria Santiloni, Fernanda da Cruz Landim-Alvarenga
Cryopreservation is a feasible alternative to maintaining several cell lines, particularly for immediate therapeutic use, transportation of samples and implementation of new in vitro studies. This work parts from the hypothesis that the medium of cryopreservation composed by 90% of conditioned medium (CM) supports cryopreservation of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs), allowing the maintenance of the biological properties for the establishment of cell banks intended for therapeutic use and in vitro studies...
November 26, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27882356/intra-osseous-co-transplantation-of-cd34-selected-umbilical-cord-blood-and-mesenchymal-stromal-cells
#7
Leland Metheny, Saada Eid, Karen Lingas, Jane Reese, Howard Meyerson, Alexander Tong, Marcos de Lima, Alex Y Huang
Human mesenchymal stromal cells (MSC) have been shown to support the growth and differentiation of hematopoietic stem cells (HSC). We hypothesized that intra-osseous (IO) co-transplantation of MSC and umbilical cord blood (UCB) may be effective in improving early HSC engraftment, as IO transplantation has been demonstrated to enhance UCB engraftment in NOD SCID-gamma (NSG) mice. Following non-lethal irradiation (300rads), 6 groups of NSG mice were studied: 1) intravenous (IV) UCB CD34(+) cells, 2) IV UCB CD34(+) cells and MSC, 3) IO UCB CD34(+) cells, 4) IO UCB CD34(+) cells and IO MSC, 5) IO UCB CD34(+) cells and IV MSC, and 6) IV UCB CD34(+) and IO MSC...
2016: Hematol Med Oncol
https://www.readbyqxmd.com/read/27882158/functional-characterization-of-human-umbilical-cord-derived-mesenchymal-stem-cells-for-treatment-of-systolic-heart-failure
#8
Zhihua Fang, Xiaoguang Yin, Jianzhong Wang, Na Tian, Qiang Ao, Yongquan Gu, Ying Liu
Congestive heart failure (HF) is a leading cause of morbidity and mortality worldwide. Although advances in medical therapy, mechanical support and heart transplantation have been made, almost half of all patients with HF succumb to the disease within five years of the initial diagnosis. Therefore, treatment methods need to be identified to restore the structure and function of cardiac muscle. Three patients with HF caused by ischemic cardiomyopathy received human umbilical cord-derived mesenchymal stem cell (HUC-MSC) intravenous infusion were included in the present study...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27882104/safety-and-therapeutic-effect-of-mesenchymal-stem-cell-infusion-on-moderate-to-severe-ulcerative-colitis
#9
Jianxia Hu, Gang Zhao, Lize Zhang, Cuixia Qiao, Aiping Di, Hong Gao, Hong Xu
One of the primary targets of the clinical treatment of ulcerative colitis (UC) is to repair the damaged colonic mucosa. Mesenchymal stem cells (MSCs) have therapeutic potential in regenerative medicine due to their differentiation capacity and their secretion of numerous bioactive molecules. The present study describes a clinical trial (trial registration no. NCT01221428) investigating the safety and therapeutic effect of MSCs derived from human umbilical cord on moderate to severe UC. Thirty-four patients with UC were included in group I and treated with MSC infusion in addition to the base treatment, and thirty-six patients were in group II and treated with normal saline in addition to the base treatment...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27874233/induction-of-hepatocyte-like-cells-from-human-umbilical-cord-derived-mesenchymal-stem-cells-by-defined-micrornas
#10
Xia Zhou, Lina Cui, Xinmin Zhou, Qiong Yang, Lu Wang, Guanya Guo, Yu Hou, Weile Cai, Zheyi Han, Yongquan Shi, Ying Han
Generating functional hepatocyte-like cells (HLCs) from mesenchymal stem cells (MSCs) is of great urgency for bio-artificial liver support system (BALSS). Previously, we obtained HLCs from human umbilical cord-derived MSCs by overexpressing seven microRNAs (HLC-7) and characterized their liver functions in vitro and in vivo. Here, we aimed to screen out the optimal miRNA candidates for hepatic differentiation. We sequentially removed individual miRNAs from the pool and examined the effect of transfection with remainder using RT-PCR, periodic acid-Schiff (PAS) staining and low-density lipoprotein (LDL) uptake assays and by assessing their function in liver injury models...
November 22, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27874039/effects-of-light-emitting-diode-irradiation-on-the-osteogenesis-of-human-umbilical-cord-mesenchymal-stem-cells-in-vitro
#11
Dazhi Yang, Weihong Yi, Ertian Wang, Min Wang
The aim of this study was to examine the effects of light-emitting diode (LED) photobiomodulation therapy on the proliferation and differentiation of human umbilical cord mesenchymal stem cells (hUMSCs) cultured in osteogenic differentiation medium. HUMSCs were irradiated with an LED light at 620 nm and 2 J/cm(2) and monitored for cell proliferation and osteogenic differentiation activity. The experiment involved four groups of cells: the control group; the osteogenic group (osteo group); the LED group; the osteogenic + LED group (LED + osteo group)...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27873515/angiopoietin-1-modified-human-umbilical-cord-mesenchymal-stem-cell-therapy-for-endotoxin-induced-acute-lung-injury-in-rats
#12
Zhi Wei Huang, Ning Liu, Dong Li, Hai Yan Zhang, Ying Wang, Yi Liu, Le Ling Zhang, Xiu Li Ju
PURPOSE: Angiopoietin-1 (Ang1) is a critical factor for vascular stabilization and endothelial survival via inhibition of endothelial permeability and leukocyte- endothelium interactions. Hence, we hypothesized that treatment with umbilical cord mesenchymal stem cells (UCMSCs) carrying the Ang1 gene (UCMSCs-Ang1) might be a potential approach for acute lung injury (ALI) induced by lipopolysaccharide (LPS). MATERIALS AND METHODS: UCMSCs with or without transfection with the human Ang1 gene were delivered intravenously into rats one hour after intra-abdominal instillation of LPS to induce ALI...
January 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/27864711/mesenchymal-stem-cells-derived-exosomal-micrornas-contribute-to-wound-inflammation
#13
REVIEW
Dongdong Ti, Haojie Hao, Xiaobing Fu, Weidong Han
Clinical and experimental studies have highlighted the significance of inflammation in coordinating wound repair and regeneration. However, it remains challenging to control the inflammatory response and tolerance at systemic levels without causing toxicity to injured tissues. Mesenchymal stem cells (MSCs) possess potent immunomodulatory properties and facilitate tissue repair by releasing exosomes, which generate a suitable microenvironment for inflammatory resolution. Exosomes contain several effective bioactive molecules and act as a cell-cell communication vehicle to influence cellular activities in recipient cells...
November 18, 2016: Science China. Life Sciences
https://www.readbyqxmd.com/read/27862947/osteogenic-potential-of-stem-cells-seeded-bioactive-nanocomposite-scaffolds-a-comparative-study-between-human-mesenchymal-stem-cells-derived-from-bone-umbilical-cord-wharton-s-jelly-and-adipose-tissue
#14
Saeid Kargozar, Masoud Mozafari, Seyed Jafar Hashemian, Peiman Brouki Milan, Sepideh Hamzehlou, Mansooreh Soleimani, Mohammad Taghi Joghataei, Mazaher Gholipourmalekabadi, Alireza Korourian, Kazem Mousavizadeh, Alexander M Seifalian
Bone regeneration is considered as an unmet clinical need, the aim of this study is to investigate the osteogenic potential of three different mesenchymal stem cells (MSCs) derived from human bone marrow (BM-MSCs), umbilical cord Wharton's jelly (UC-MSCs), and adipose (AD-MSCs) seeded on a recently developed nanocomposite scaffold (bioactive glass/gelatin) implanted in rat animal models with critical size calvarial defects. In this study, after isolation, culture, and characterization, the MSCs were expanded and seeded on the scaffolds for in vitro and in vivo studies...
November 15, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27861182/cryopreserved-xeno-free-human-umbilical-cord-mesenchymal-stromal-cells-reduce-lung-injury-severity-and-bacterial-burden-in-rodent-escherichia-coli-induced-acute-respiratory-distress-syndrome
#15
Gerard F Curley, Mirjana Jerkic, Steve Dixon, Grace Hogan, Claire Masterson, Daniel O'Toole, James Devaney, John G Laffey
OBJECTIVE: Although mesenchymal stem/stromal cells represent a promising therapeutic strategy for acute respiratory distress syndrome, clinical translation faces challenges, including scarcity of bone marrow donors, and reliance on bovine serum during mesenchymal stem/stromal cell proliferation. We wished to compare mesenchymal stem/stromal cells from human umbilical cord, grown in xeno-free conditions, with mesenchymal stem/stromal cells from human bone marrow, in a rat model of Escherichia coli pneumonia...
November 16, 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27853939/wharton-s-jelly-mesenchymal-stromal-cells-as-a-feeder-layer-for-the-ex-vivo-expansion-of-hematopoietic-stem-and-progenitor-cells-a-review
#16
REVIEW
Melania Lo Iacono, Rita Anzalone, Giampiero La Rocca, Elena Baiamonte, Aurelio Maggio, Santina Acuto
In recent years, umbilical cord blood (UCB) has been widely used as an alternative source to bone marrow (BM) for transplantation of hematopoietic stem and progenitor cells (HSPCs) in a variety of hematological and non-hematological disorders. Nevertheless, the insufficient number of UCB-HSPCs for graft represents a major challenge. HSPCs ex vivo expansion prior to transplantation is a valid strategy to overcome this limit. Several attempts to optimize the expansion conditions have been reported, including the use of mesenchymal stromal cells (MSCs) as feeder layer...
November 16, 2016: Stem Cell Reviews
https://www.readbyqxmd.com/read/27852316/defined-three-dimensional-culture-conditions-mediate-efficient-induction-of-definitive-endoderm-lineage-from-human-umbilical-cord-wharton-s-jelly-mesenchymal-stem-cells
#17
Ashraf Al Madhoun, Hamad Ali, Sarah AlKandari, Valerie Lopez Atizado, Nadeem Akhter, Fahd Al-Mulla, Maher Atari
BACKGROUND: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) are gaining increasing interest as an alternative source of stem cells for regenerative medicine applications. Definitive endoderm (DE) specification is a prerequisite for the development of vital organs such as liver and pancreas. Hence, efficient induction of the DE lineage from stem cells is crucial for subsequent generation of clinically relevant cell types. Here we present a defined 3D differentiation protocol of WJ-MSCs into DE cells...
November 16, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27849398/production-of-endothelial-progenitor-cells-obtained-from-human-wharton-s-jelly-using-different-culture-conditions
#18
S A Zayed, T M Gaafar, R M Samy, D Sabry, A S Nasr, Fa Abdel Maksoud
Endothelial progenitor cells (EPC) participate in revascularization and angiogenesis. EPC can be cultured in vitro from mononuclear cells of peripheral blood, umbilical cord blood or bone marrow; they also can be transdifferentiated from mesenchymal stem cells (MSC). We isolated EPCs from Wharton's jelly (WJ) using two methods. The first method was by obtaining MSC from WJ and characterizing them by flow cytometry and their adipogenic and osteogenic differentiation, then applying endothelial growth differentiating media...
November 2016: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/27837561/banking-of-adipose-and-cord-tissue-derived-stem-cells-technical-and-regulatory-issues
#19
David T Harris
Stem cells are found in all multicellular organisms and are defined as cells that can differentiate into specialized mature cells as well as divide to produce more stem cells. Mesenchymal stem cells (MSC) were among the first stem cell types to be utilized for regenerative medicine. Although initially isolated from bone marrow, based on ease and costs of procurement, MSC derived from adipose tissue (AT-MSC) and umbilical cord tissue (CT-MSC) are now preferred stem cell sources for these applications. Both adipose tissues and cord tissue present unique problems for biobanking however, in that these are whole tissues, not cellular suspensions...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27833846/mesenchymal-stromal-cells-derived-from-whole-human-umbilical-cord-exhibit-similar-properties-to-those-derived-from-wharton-s-jelly-and-bone-marrow
#20
Claire Mennan, Sharon Brown, Helen McCarthy, Eleni Mavrogonatou, Dimitris Kletsas, John Garcia, Birender Balain, James Richardson, Sally Roberts
Mesenchymal stromal cells (MSC) can be isolated from several regions of human umbilical cords, including Wharton's jelly (WJ), artery, vein or cord lining. These MSC appear to be immune privileged and are promising candidates for cell therapy. However, isolating MSC from WJ, artery, vein or cord lining requires time-consuming tissue dissection. MSC can be obtained easily via briefly digesting complete segments of the umbilical cord, likely containing heterogenous or mixed populations of MSC (MC-MSC). MC-MSC are generally less well characterized than WJ-MSC, but nevertheless represent a potentially valuable population of MSC...
November 2016: FEBS Open Bio
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