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https://www.readbyqxmd.com/read/28716025/beta-mangostin-demonstrates-apoptogenesis-in-murine-leukaemia-wehi-3-cells-in-vitro-and-in-vivo
#1
Fatima Abdelmutaal Ahmed Omer, Najihah Mohd Hashim, Mohamed Yousif Ibrahim, Abdulmannan F Aldoubi, Pouya Hassandarvish, Firouzeh Dehghan, Noraziah Nordin, Hamed Karimian, Landa Zeenelabdin Ali Salim, Mahmood Ameen Abdulla, Karim Al-Jashamy, Syam Mohan
BACKGROUND: Beta-mangostin (BM) is a xanthone-type of natural compound isolated from Cratoxylum arborescens. This study aimed to examine the apoptosis mechanisms induced by BM in a murine monomyelocytic cell line (WEHI-3) in vitro and in vivo. METHODS: A WEHI-3 cell line was used to evaluate the cytotoxicity of BM by MTT. AO/PI and Hoechst 33342 dyes, Annexin V, multiparametric cytotoxicity 3 by high content screening (HCS); cell cycle tests were used to estimate the features of apoptosis and BM effects...
July 17, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28702018/a-mutant-isoform-of-obge-causes-cell-death-by-interfering-with-cell-division
#2
Liselot Dewachter, Natalie Verstraeten, Michiel Jennes, Tom Verbeelen, Jacob Biboy, Daniel Monteyne, David Pérez-Morga, Kevin J Verstrepen, Waldemar Vollmer, Maarten Fauvart, Jan Michiels
Cell division is a vital part of the cell cycle that is fundamental to all life. Despite decades of intense investigation, this process is still incompletely understood. Previously, the essential GTPase ObgE, which plays a role in a myriad of basic cellular processes (such as initiation of DNA replication, chromosome segregation, and ribosome assembly), was proposed to act as a cell cycle checkpoint in Escherichia coli by licensing chromosome segregation. We here describe the effect of a mutant isoform of ObgE (ObgE(∗)) that causes cell death by irreversible arrest of the cell cycle at the stage of cell division...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28694810/oxidized-hemoglobin-is-antigenic-and-immunogenic-in-lupus
#3
Sonia Jain, Anjali Bose, Banajit Bastia, Hritika Sharma, Ruchi Sachdeva, Arun K Jain, Rahul Pal
Hemolysis-associated anemia is characteristic of diseases such as atherosclerosis, lupus, malaria, and leishmaniasis; the toxic effects of free hemoglobin (Hb) have been extensively described. This study was based on the premise that release of this sequestered, inflammatory molecule can result in deleterious immunological consequences, particularly in the context of pre-existing lupus. IgG anti-Hb responses were detected in the sera of lupus patients. Lupus-prone mice exhibited heightened plasma Hb levels, and ferric (Fe(3+)) Hb triggered preferential release of lupus-associated cytokines from splenocytes derived from aging lupus-prone mice...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28692738/inhibition-of-monocyte-chemoattractant-protein-1-prevents-conjunctival-fibrosis-in-an-experimental-model-of-glaucoma-filtration-surgery
#4
Rachel Shujuan Chong, Ying Shi Lee, Stephanie Wai Ling Chu, Li Zhen Toh, Tina Tzee Ling Wong
Purpose: To evaluate the effect of treatment with monocyte chemoattractant protein-1 receptor inhibitor (MCP-Ri) to maintain bleb survival and prevent fibrosis in an experimental model of glaucoma filtration surgery (GFS). Methods: GFS was performed on one eye of C57/Bl6 mice (n = 36) that was treated with MCP-Ri, mitomycin-C (MMC), or vehicle at the time of surgery. Real-time polymerase chain reaction was used to evaluate conjunctival expression of monocyte chemoattractant protein-1 (MCP-1), TGFB1, TGFB2, collagen 1a1 (Col1a1), sparc (Sparc), and fibronectin at 2 and 7 days following surgery...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28687020/chemotherapy-administration-to-breast-cancer-patients-affects-extracellular-vesicles-thrombogenicity-and-function
#5
Anat Aharon, Anni Sabbah, Shahar Ben-Shaul, Hila Berkovich, David Loven, Benjamin Brenner, Gil Bar-Sela
Breast cancer (BC) is the most prevalent type of malignancy in women. Extracellular vesicles (EVs) are subcellular membrane blebs that include exosomes and microparticles. STUDY AIMS: To elucidate the effects of chemotherapy administration on BC patients' EVs characteristics and their effects on endothelial cells (EC) functions. METHODS: EVs were isolated from the blood samples of 54 BC patients treated by chemotherapy (25 neo-adjuvant, 29 adjuvant) and from 20 healthy women (control group)...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28686482/outer-membrane-vesicles-blebbing-contributes-to-b-vulgatus-mpk-mediated-immune-response-silencing
#6
Jan Kevin Maerz, Alex Steimle, Anna Lange, Annika Bender, Birgit Fehrenbacher, Julia-Stefanie Frick
The Gram negative intestinal symbiont Bacteroides vulgatus mpk is able to prevent from induction of colonic inflammation in Rag1(-/-) mice and promotes immune balance in Il2(-/-) mice. These inflammation-silencing effects are associated with B. vulgatus mpk-mediated induction of semi-mature dendritic cells, especially in the colonic lamina propria (cLP). However the beneficial interaction of bacteria with host immune cells is limited due to the existence of a large mucus layer covering the intestinal epithelium...
July 7, 2017: Gut Microbes
https://www.readbyqxmd.com/read/28685254/kh-tfmdi-a-novel-sirtuin-inhibitor-alters-the-cytoskeleton-and-mitochondrial-metabolism-promoting-cell-death-in-leishmania-amazonensis
#7
Brunno Renato Farias Verçoza, Joseane Lima Prado Godinho, Sara Teixeira de Macedo-Silva, Kilian Huber, Franz Bracher, Wanderley de Souza, Juliany Cola Fernandes Rodrigues
Treatment of leishmaniasis involves the use of antimonials, miltefosine, amphotericin B or pentamidine. However, the side effects of these drugs and the reports of drug-resistant parasites demonstrate the need for new treatments that are safer and more efficacious. Histone deacetylase inhibitors are a new class of compounds with potential to treat leishmaniasis. Herein, we evaluated the effects of KH-TFMDI, a novel histone deacetylase inhibitor, on Leishmania amazonensis promastigotes and intracellular amastigotes...
July 6, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28684288/extracellular-vesicles-in-lung-disease
#8
REVIEW
Hiroshi Kubo
Accumulating evidence suggests that extracellular vesicles play a role in pathogenesis of lung diseases. The vesicles include exosomes, ectosomes (a.k.a. microparticles, extracellular vesicles, microvesicles, and shedding vesicles), and apoptotic bodies. Exosomes are generated by inward budding of the membrane (endocytosis), subsequent forming multivesicular bodies, and releasing by exocytosis. Ectosomes are formed by outward blebbing from the plasma membrane and then released by proteolytic cleavage from the cell surface...
July 3, 2017: Chest
https://www.readbyqxmd.com/read/28672157/the-p2x7-receptor-is-an-upstream-regulator-of-dynamic-blebbing-and-a-pluripotency-marker-in-human-embryonic-stem-cells
#9
Nikki Jo-Hao Weng, Prue Talbot
New methods are needed to reduce dynamic blebbing which inhibits cell attachment and survival during passaging of pluripotent stem cells. We tested the hypothesis that activation of the P2X7 receptor by extracellular ATP during passaging initiates dynamic blebbing. The P2X7 receptor was present in human embryonic stem cells (hESC), but not in differentiating cells. Extracellular ATP concentrations were 14× higher in medium during passaging. Addition of ATP to culture medium prolonged dynamic blebbing and inhibited attachment...
June 16, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28669393/eryptosis-in-health-and-disease-a-paradigm-shift-towards-understanding-the-patho-physiological-implications-of-programmed-cell-death-of-erythrocytes
#10
REVIEW
Syed M Qadri, Rosi Bissinger, Ziad Solh, Per-Arne Oldenborg
During the course of their natural ageing and upon injury, anucleate erythrocytes can undergo an unconventional apoptosis-like cell death, termed eryptosis. Eryptotic erythrocytes display a plethora of morphological alterations including volume reduction, membrane blebbing and breakdown of the membrane phospholipid asymmetry resulting in phosphatidylserine externalization which, in turn, mediates their phagocytic recognition and clearance from the circulation. Overall, the eryptosis machinery is tightly orchestrated by a wide array of endogenous mediators, ion channels, membrane receptors, and a host of intracellular signaling proteins...
June 17, 2017: Blood Reviews
https://www.readbyqxmd.com/read/28669026/a-pilot-study-on-ocular-safety-and-efficacy-of-infliximab-as-an-antifibrotic-agent-after-experimental-glaucoma-filtration-surgery
#11
Eleni Nikita, Alexandre Moulin, Ioannis Vergados, Dimitrios Brouzas, Panagiotis G Theodossiadis
INTRODUCTION: Tumor necrosis factor-α (TNF-α) is a multifunctional, proinflammatory cytokine that mediates pleiotropic biological functions, especially inflammation and immunoregulation. We hypothesized that blocking TNF-α with a monoclonal antibody would decrease inflammation and subconjunctival scarring in an animal model of experimental filtration surgery. METHODS: In a randomized, prospective, masked-observer study, 30 New Zealand albino rabbits underwent glaucoma filtration surgery...
July 1, 2017: Ophthalmology and Therapy
https://www.readbyqxmd.com/read/28659878/detection-and-physicochemical-characterization-of-membrane-vesicles-mvs-of-lactobacillus-reuteri-dsm-17938
#12
Rossella Grande, Christian Celia, Gabriella Mincione, Annarita Stringaro, Luisa Di Marzio, Marisa Colone, Maria C Di Marcantonio, Luca Savino, Valentina Puca, Roberto Santoliquido, Marcello Locatelli, Raffaella Muraro, Luanne Hall-Stoodley, Paul Stoodley
Membrane vesicles (MVs) are bilayer structures which bleb from bacteria, and are important in trafficking biomolecules to other bacteria or host cells. There are few data about MVs produced by the Gram-positive commensal-derived probiotic Lactobacillus reuteri; however, MVs from this species may have potential therapeutic benefit. The aim of this study was to detect and characterize MVs produced from biofilm (bMVs), and planktonic (pMVs) phenotypes of L. reuteri DSM 17938. MVs were analyzed for structure and physicochemical characterization by Scanning Electron Microscope (SEM) and Dynamic Light Scattering (DLS)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28655149/neuromodulation-of-axon-terminals
#13
Darpan Chakraborty, Dennis Q Truong, Marom Bikson, Hanoch Kaphzan
Understanding which cellular compartments are influenced during neuromodulation underpins any rational effort to explain and optimize outcomes. Axon terminals have long been speculated to be sensitive to polarization, but experimentally informed models for CNS stimulation are lacking. We conducted simultaneous intracellular recording from the neuron soma and axon terminal (blebs) during extracellular stimulation with weak sustained (DC) uniform electric fields in mouse cortical slices. Use of weak direct current stimulation (DCS) allowed isolation and quantification of changes in axon terminal biophysics, relevant to both suprathreshold (e...
June 24, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28651590/effect-of-different-incision-sites-of-phacoemulsification-on-trabeculectomy-bleb-function-prospective-case-control-study
#14
Mohamed Anbar, Hatem Ammar
BACKGROUND: The aim of this study was to compare superior and temporal clear corneal incisions of uneventful phacoemulsification and in-the-bag intraocular lens implantation on intraocular pressure control and the bleb morphology in eyes that have undergone previous successful trabeculectomy. METHODS: In this Prospective case-control study, a total of 100 eyes of 100 patients previously undergone trabeculectomy without antimetabolites, divided into two groups. Group A (temporal group) including 50 patients underwent phacoemulsification with a temporal corneal incision and group B (superior group) including 50 patients underwent phacoemulsification with a superior corneal incision...
June 26, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28640115/management-of-neovascular-glaucoma-with-intravitreal-ranibizumab-panretinal-photocoagulation-and-subsequent-5-fluorouracil-augmented-trabeculectomy-a-case-report
#15
Xinyue Zhao, Zhi Wang, Xiaobo Yang
RATIONALE: Neovascular glaucoma (NVG) is one of the most refractory types of glaucoma caused by high ischemic retinal disorders, resulting in severe visual loss. Intravitreal injections of ranibizumab have been reported to have anatomical and functional success in treating NVG. PATIENT CONCERNS-DIAGNOSES-INTERVENTIONS: We report a case with neovascular glaucoma due to central retinal vein occlusion who received combination therapy with intravitreal ranibizumab (IVR) injection, panretinal photocoagulation (PRP) and subsequent 5-fluorouracil (5-FU) augmented trabeculectomy...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28626816/evaluation-of-topical-bevacizumab-as-an-adjunct-to-mitomycin-c-augmented-trabeculectomy
#16
Reza Zarei, Masoumeh Masoumpour, Sasan Moghimi, Ghasem Fakhraei, Yadollah Eslami, Masoud Mohammadi
PURPOSE: To investigate the safety and synergistic effect of topical bevacizumab after trabeculectomy surgery with mitomycin C (MMC). METHODS: In this prospective, non-randomized, comparative interventional study, 40 eyes from 40 patients with uncontrolled open-angle glaucoma were studied after they underwent primary trabeculectomy with mitomycin C (0.02% for 2 min). Following the procedure topical bevacizumab (4 mg/mL) was used for 2 weeks 4 times daily in group A...
June 2017: Journal of Current Ophthalmology
https://www.readbyqxmd.com/read/28626446/gnrh-induces-erk-dependent-bleb-formation-in-gonadotrope-cells-involving-recruitment-of-members-of-a-gnrh-receptor-associated-signalosome-to-the-blebs
#17
Liat Rahamim-Ben Navi, Anna Tsukerman, Alona Feldman, Philippa Melamed, Melanija Tomić, Stanko S Stojilkovic, Ulrich Boehm, Rony Seger, Zvi Naor
We have previously described a signaling complex (signalosome) associated with the GnRH receptor (GnRHR). We now report that GnRH induces bleb formation in the gonadotrope-derived LβT2 cells. The blebs appear within ~2 min at a turnover rate of ~2-3 blebs/min and last for at least 90 min. Formation of the blebs requires active ERK1/2 and RhoA-ROCK but not active c-Src. Although the following ligands stimulate ERK1/2 in LβT2 cells: EGF > GnRH > PMA > cyclic adenosine monophosphate (cAMP), they produced little or no effect on bleb formation as compared to the robust effect of GnRH (GnRH > PMA > cAMP > EGF), indicating that ERK1/2 is required but not sufficient for bleb formation possibly due to compartmentalization...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28624462/evaluation-on-antiviral-activity-of-coumarin-derivatives-against-spring-viraemia-of-carp-virus-in-epithelioma-papulosum-cyprini-cells
#18
Lei Liu, Yang Hu, Yu-Feng Shen, Gao-Xue Wang, Bin Zhu
As one of the most serious pathogens in the freshwater aquatic environment, spring viraemia of carp virus (SVCV) induces a high mortality rate in several cyprinid fishes. In this study, we designed and synthesized a total of 44 coumarin derivatives to evaluate the anti-SVCV activity. By comparing the inhibitory concentration at half-maximal activity (IC50), two imidazole coumarins (B4 and C2) were selected, with maximum inhibitory rates on SVCV more than 90%. Mechanistically, B4 or C2 did not affect viral adhesion and delivery from endosomes to the cytosol...
June 15, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28617656/sarcocystis-pantherophis-n-sp-from-eastern-rat-snakes-pantherophis-alleghaniensis-definitive-hosts-and-interferon-gamma-gene-knockout-mice-as-experimental-intermediate-hosts
#19
Shiv Verma, David Lindsay, Joseph Mowery, Benjamin Rosenthal, J P Dubey
Here we report a new species, Sarcocystis pantherophisi n. sp. with the Eastern rat snake (Pantherophis alleghaniensis) as natural definitive host and the interferon gamma gene knockout (KO) mouse as the experimental intermediate host. Sporocysts (n = 15) from intestinal contents of the snake were 17.3 x 10.9 µm. Sporocysts were orally infective to interferon gamma KO mice but not to laboratory raised albino outbred house mice (Mus musculus). The interferon gamma KO mice developed schizont-associated neurological signs and schizonts were cultivated in vitro from the brain...
June 15, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28615876/microscopic-investigation-to-determine-the-effect-of-beauveria-bassiana-bals-vuill-and-cymbopogon-citratus-dc-stapf-treatment-on-different-life-stages-of-musca-domestica-l
#20
Sapna Mishra, Peeyush Kumar, Anushree Malik
Microscopic investigation was done to determine the effect of entomopathogenic fungi, Beauveria bassiana and essential oil of Cymbopogon citratus on different life stages of Musca domestica. Scanning electron microscopy investigation of fungal infected larvae showed sluggish movement, rigor, and failure of body to balance in water. Treated larvae also revealed varied level of cuticle shrinkage and extreme dehydration. Surface of B. bassiana infected pupae showed varied stage of mycelial growth, while the cadaver of adult fly was observed to have extensive fungal growth covering their entire body surface...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
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