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Sarah Sheard, Joanna Moser, Charlie Sayer, Konstantinos Stefanidis, Anand Devaraj, Ioannis Vlahos
Early lung cancers associated with cystic airspaces are increasingly being recognized as a cause of delayed diagnoses-owing to data gathered from screening trials and encounters in routine clinical practice as more patients undergo serial imaging. Several morphologic subtypes of cancers associated with cystic airspaces exist and can exhibit variable patterns of progression as the solid elements of the tumor grow. Current understanding of the pathogenesis of these malignancies is limited, and the numbers of cases reported in the literature are small...
April 13, 2018: Radiographics: a Review Publication of the Radiological Society of North America, Inc
S Tammy Hsu, Hesham Gabr, Christian Viehland, Karim Sleiman, Hoan T Ngo, Oscar M Carrasco-Zevallos, Lejla Vajzovic, Ryan P McNabb, Sandra S Stinnett, Joseph A Izatt, Anthony N Kuo, Cynthia A Toth
Purpose: We advance studies of subretinal treatments by developing a microscope-integrated optical coherence tomography (MIOCT) image-based method for measuring the volume of therapeutics delivered into the subretinal space. Methods: A MIOCT image-based volume measurement method was developed and assessed for accuracy and reproducibility by imaging an object of known size in model eyes. This method then was applied to subretinal blebs created by injection of diluted triamcinolone...
April 2018: Translational Vision Science & Technology
Li-Fong Seet, Li Zhen Toh, Stephanie Chu, Sharon N Finger, Florent Ginhoux, Wanjin Hong, Tina T Wong
Purpose: We determine the effects of bevacizumab on collagen production in a mouse model of conjunctival scarring. Methods: Experimental surgery was performed as described for the mouse model of conjunctival scarring, and bevacizumab was introduced by conjunctival injection. The capacity of bevacizumab to recognize conjunctival VEGF-A was determined by ELISA. Col1a1 was measured by real-time PCR and immunoblotting. T cells and collagen were visualized by immunofluorescence and picrosirius red staining of bleb cryosections...
March 1, 2018: Investigative Ophthalmology & Visual Science
Trevor J McGill, Jonathan Stoddard, Lauren M Renner, Ilhem Messaoudi, Kapil Bharti, Shoukhrat Mitalipov, Andreas Lauer, David J Wilson, Martha Neuringer
Purpose: To characterize the intraocular immune response following transplantation of iPS-derived allogeneic RPE cells into the subretinal space of non-immune-suppressed rhesus macaques. Methods: GFP-labeled allogeneic iPS-derived RPE cells were transplanted into the subretinal space of one eye (n = 6), and into the contralateral eye 1 day to 4 weeks later, using a two-stage transretinal and transscleral approach. Retinas were examined pre- and post-surgery by color fundus photography, fundus autofluorescence, and optical coherence tomography (OCT) imaging...
March 1, 2018: Investigative Ophthalmology & Visual Science
Jhi Biau Foo, May Lee Low, Ji Hui Lim, Yan Zhi Lor, Rusyidah Zainol Abidin, Vilasini Eh Dam, Napsiah Abdul Rahman, Chaw Yee Beh, Lee Chin Chan, Chee Wun How, Yin Sim Tor, Latifah Saiful Yazan
Copper complexes have been widely studied for the anti-tumour application as cancer cells are reported to take up greater amounts of copper than normal cells. Preliminary study revealed that the newly synthesised copper complex [Cu(SBCM)2 ] displayed marked anti-proliferative towards triple-negative MDA-MB-231 breast cancer cells. Therefore, Cu(SBCM)2 has great potential to be developed as an agent for the management of breast cancer. The present study was carried out to investigate the mode of cell death induced by Cu(SBCM)2 towards MDA-MB-231 breast cancer cells...
April 5, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Sangkyun Cho, Amal Abbas, Jerome Irianto, Irena Ivanovska, Yuntao Xia, Manu Tewari, Dennis E Discher
Interphase phosphorylation of lamin-A,C depends dynamically on a cell's microenvironment, including the stiffness of extracellular matrix. However, phosphorylation dynamics is poorly understood for diseased forms such as progerin, a permanently farnesylated mutant of LMNA that accelerates aging of stiff and mechanically stressed tissues. Here, fine-excision alignment mass spectrometry (FEA-MS) is developed to quantify progerin and its phosphorylation levels in patient iPS cells differentiated to mesenchymal stem cells (MSCs)...
April 5, 2018: Nucleus
Peixin He, Ke Wang, Yingli Cai, Xiaolong Hu, Yan Zheng, Junjie Zhang, Wei Liu
Sclerotial formation is a key phase of the morel life cycle and lipids have been recorded as the main cytoplasmic reserves in sclerotia of Morchella fungi without any experimental verification. In this study, the ultrastructural features of the undifferentiated mycelia stage (MS) and three main sclerotial differentiation states (sclerotial initial [SI], sclerotial development [SD] and sclerotial maturation [SM]) were compared by transmission electron microscopy. The nature of the energy-rich substance in hypha and sclerotium of Morchella importuna was qualitatively investigated by confocal laser scanning microscopy and quantitatively studied by extraction of lipids...
March 21, 2018: Micron: the International Research and Review Journal for Microscopy
Julien Aron, Aymeric Couturier, Leila Sinayoko, Viviane Duedal, Christophe Ridel, Maxime Touzot
Iron deficiency caused by gastrointestinal (GI) bleeding is a common cause of anemia in hemodialysis patient. Herein, we report the case of an 89-year-old hemodialysis patient who presented with acute anemia and melena. Endoscopy found gastritis and diverticulosis without active bleeding. A capsule endoscopy (CE) was then performed and revealed multiple vascular lesions that lead to the diagnosis of Blue Rubber Bleb Nevus syndrome (BRBNS). This rare disease is associated with multiple venous malformations in the skin and the GI tract, usually observed in children...
April 2, 2018: Hemodialysis International
Yuxue Sun, Jing Yu, Xinrui Liu, Chaochao Zhang, Junguo Cao, Guihong Li, Xingxing Liu, Yong Chen, Haiyan Huang
Gliomas, the most common primary malignant brain tumor, exhibit high metabolic activity. The targeting of metabolism alterations, particularly in mitochondria, is emerging as an efficient approach for curing cancers. Here, we showed that berberine, a natural compound that is used as an antibacterial agent, could reduce cellular viability and induce oncosis-like death, characterized by cell swelling, cytoplasmic vacuoles and plasma membrane blebbing, in gliomas, and that these effects were correlated with intracellular adenosine triphosphate (ATP) depletion...
March 28, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
P H Aussedat, S Laroumagne, H Dutau, P Astoul
Bean's syndrome ('blue rubber bleb nevus syndrome') is a rare disease characterized by venous malformations involving various organs. Most often these lesions are localized to the skin and the digestive system. Gastro-intestinal bleeding is the most frequent presentation. Though other organs can be affected, chest localizations are infrequent and pleural localization is exceptional. We report the case of an asbestos-exposed patient with Bean's syndrome with characteristic skin lesions, smoker, hospitalized for the investigation of a hemorrhagic pleural effusion...
March 28, 2018: Revue des Maladies Respiratoires
Mehmet Yakin, Umit Eksioglu, Zuleyha Yalniz-Akkaya, Selma Uzman, Evin Singar-Ozdemir, Kemal Gultekin, Gulizar Demirok, Ilgaz Yalvac, Firdevs Ornek, Ayse Burcu
PURPOSE: To assess and compare the outcomes of trabeculectomy with and without antimetabolites (AMs) and glaucoma drainage devices (GDDs) in the management of elevated intraocular pressure (IOP) after penetrating keratoplasty (PK). METHODS: Data of 84 eyes of 81 patients who underwent trabeculectomy (12 eyes without an AM and 42 eyes with an AM) or GDD implantation (30 eyes) after PK were reviewed retrospectively. The main outcome measures were IOP control, corneal graft survival, and postoperative ocular complications...
March 28, 2018: Cornea
Ali S Al-Beshri, Deepak P Edward, Earl Randy Craven
PURPOSE: To determine if glaucoma filtering blebs migrate over or under the cornea epithelium using histopathological and immunohistochemical (IHC) techniques to evaluate the likely origin of the surface epithelium and bleb matrix. METHODS: Histologic and IHC evaluations were performed of normal conjunctiva (n=4,) corneal overhanging-dissecting blebs (n=4,) and leaking blebs over the scleral surface (n=6.) Antibodies were used against epithelial (CK3+12, CK13) and extracellular matrix (ECM) (decorin and keratan sulfate) antigens...
March 27, 2018: Journal of Glaucoma
Sofia Fili, Sabine Seddig, Markus Kohlhaas
BACKGROUND: The Ologen implant consists of a biodegradable collagen and glycosaminoglycan and is intended to avoid postoperative bleb scarring or encapsulation. MATERIALS AND METHODS: In a retrospective clinical study, 806 patients with advanced glaucoma were treated with trabeculectomy combined with mitomycin C0.02% and Ologen implant. The follow-up time of the study was 8 years. RESULTS: A long-term reduction of the intraocular pressure below 16 mmHg was achieved in 92...
March 28, 2018: Klinische Monatsblätter Für Augenheilkunde
Maitham A Khajah, Princy M Mathew, Yunus A Luqmani
BACKGROUND: The Na+/K+-ATPase (NKP) is an important ion transporter also involved in signal transduction. Its expression profile is altered in various tumours including that of the breast. We studied the effect of inhibiting NKP activity in non-tumorigenic breast cell line and in estrogen receptor positive and negative breast cancer cells. METHODS: Expression and localization of NKP and downstream signaling molecules were determined by RT-PCR, western blotting and immunofluorescence...
2018: PloS One
E M Hoffmann, N Pfeiffer
Trabeculectomy is the most proven method for the treatment of open angle glaucoma. The long-term results of this method show an 80% success rate in lowering intraocular pressure over 20 years. The surgical technique is demonstrated in detail with the help of a video of the operation and which is available online. For more than 15 years trabeculectomy has been performed with the use of antifibrotic substances, such as mitomycin C. Long-term treatment with antiglaucoma eyedrops leads to a proven inflammation of the conjunctiva and the ocular surface with an increase in lymphocytes, mast cells, and fibroblasts...
March 20, 2018: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
Shreesha Kumar Kodavoor, Nitin Narendra Tiwari, Dandapani Ramamurthy
Purpose: The aim of this study is to describe a modified technique of using conjunctival tissue from the pterygium itself without any rotation of graft for the primary pterygium in eyes with glaucoma filtering bleb, glaucoma suspects, and in primary double-head pterygium using fibrin glue. Methods: In this retrospective, noncomparative, interventional case series, 98 eyes of 98 patients with primary pterygium operated between July 2011 to July 2016 were included...
April 2018: Indian Journal of Ophthalmology
Hsiang-Ling Kuo, Pei-Chuan Ho, Shenq-Shyang Huang, Nan-Shan Chang
A feasible design is made to measure three protein/protein interactions to visualize signal pathways by time-lapse Förster resonance energy transfer (FRET) microscopy. When interacting proteins are in close proximity, excitation energy is provided to allow the energy flow from the first molecule to excite the second, followed by energy transfer to the third. By phorbol ester/calcium ionophore stimulation, for example, a real-time complex formation of ectopic IκBα/ERK/WWOX occurs as measured by FRET microscopy, indicative of an ongoing functional signaling...
2018: Cell Death Discovery
Thi Kieu Hau Hoang, Young Kook Kim, Jin Wook Jeoung, Ki Ho Park
PURPOSE: To investigate whether there is any effect of age on bleb morphology and surgical success after trabeculectomy with adjunctive Mitomycin C (MMC). METHODS: A total 143 eyes of 123 patients were divided into two groups: group 1 ( ≤ 60 years old, n = 85) and group 2 ( > 60 years old, n = 58). Kaplan-Meier survival curve analysis was used to assess long-term surgical success according to "complete success" (intraocular pressure (IOP) reduction ≥ 30% without medications) and "qualified success" criteria (IOP reduction ≥ 30%, with or without medications) between the two groups...
March 27, 2018: Eye
Amanda J DuBose, Stephen T Lichtenstein, Noreen M Petrash, Michael R Erdos, Leslie B Gordon, Francis S Collins
LMNA encodes the A-type lamins that are part of the nuclear scaffold. Mutations in LMNA can cause a variety of disorders called laminopathies, including Hutchinson-Gilford progeria syndrome (HGPS), atypical Werner syndrome, and Emery-Dreifuss muscular dystrophy. Previous work has shown that treatment of HGPS cells with the mTOR inhibitor rapamycin or with the rapamycin analog everolimus corrects several of the phenotypes seen at the cellular level-at least in part by increasing autophagy and reducing the amount of progerin, the toxic form of lamin A that is overproduced in HGPS patients...
March 26, 2018: Proceedings of the National Academy of Sciences of the United States of America
Guadalupe Ortega-Pierres, Raúl Argüello-García, Marco S Laredo-Cisneros, Rocío Fonseca-Linán, Misael Gómez-Mondragón, Rolando Inzunza-Arroyo, David Flores-Benítez, Arturo Raya-Sandino, Bibiana Chavez-Munguía, José Luis Ventura-Gallegos, Alejandro Zentella-Dehesa, Rosa María Bermúdez-Cruz, Lorenza González-Mariscal
The adhesion of Giardia duodenalis trophozoites to intestinal epithelial cells allows the onset and maintenance of giardiasis. During these interactions, epithelial cells can be committed to apoptosis by enzymes secreted by the parasites, including cysteine proteases that are increasingly identified as virulence factors in parasitic protozoa. In this work, a monoclonal antibody (mAb1G3) raised against G. duodenalis surface components was found to react with a 25 kDa protein expressed in the cell surface and flagella of G...
March 20, 2018: International Journal for Parasitology
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