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https://www.readbyqxmd.com/read/28516118/quantitative-measurements-of-hiv-1-and-dextran-capture-by-human-monocyte-derived-dendritic-cells-mddcs
#1
Mickaël M Ménager, Dan R Littman
The aim of this protocol is to describe how to measure and quantify the amount of HIV-1 particles and dextran molecules internalized in human monocyte derived dendritic cells (MDDCs), using three different techniques: flow cytometry, quantitative PCR and confocal microscopy. BACKGROUND: This protocol was developed in order to assess the changes of HIV-1 internalization upon disruption of actin nucleation in human monocyte derived dendritic cells. Following a shRNA screen to identify genes important for HIV-1 transfer from dendritic cells to T cells, we observed that a disruption of actin nucleation leads to a switch from actin rich dendrites to blebs, due to an excess of actomyosin contraction...
November 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28511649/il-10-ameliorates-tnf-%C3%AE-induced-meniscus-degeneration-in-mature-meniscal-tissue-in-vitro
#2
P Behrendt, K Häfelein, A Preusse-Prange, A Bayer, A Seekamp, B Kurz
BACKGROUND: Joint inflammation causes meniscus degeneration and can exacerbate post-traumatic meniscus injuries by extracellular matrix degradation, cellular de-differentiation and cell death. The aim of this study was to examine whether anti-inflammatory interleukin-10 exerts protective effects in an in vitro model of TNF-α-induced meniscus degeneration. METHODS: Meniscus tissue was harvested from the knees of adult cows. After 24 h of equilibrium explants were simultaneously treated with bovine TNF-α and IL-10...
May 16, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28508973/phlegmasia-cerulean-dolens-complication-of-femoral-vein-catheterization
#3
M Hari Krishna Reddy, B Sangeetha, M Aruna, V Sarat Chandra, Anil C Venkata Kumar, R Ram, V Sivakumar
There are three less frequent manifestations of acute massive venous thrombosis and obstruction of the venous drainage of an extremity. They are phlegmasia alba dolens, phlegmasia cerulean dolens (PCD), and venous gangrene. The term PCD differentiates ischemia-associated massive venous thrombosis from phlegmasia alba dolens, which describes fulminant venous thrombosis without ischemia. We present a 55-year-old hypertensive, who presented with paedal oedema and breathlessness at rest. About a month prior to this admission, she suffered dislocation of left patella...
November 2016: CEN Case Reports
https://www.readbyqxmd.com/read/28486276/outcomes-for-ab-interno-bleb-revision-with-a-novel-translimbal-sclerostomy-spatula
#4
Davinder S Grover, Ronald L Fellman
PURPOSE: To report outcomes for a novel ab interno bleb revision technique and spatula. PATIENTS AND METHODS: This is a retrospective chart review. RESULTS: A total of 21 eyes of 21 patients underwent ab interno bleb revision with the Grover-Fellman biplanar sclerostomy spatula. Mitomycin C was given at least 1 week preoperation. The mean age of the patients and blebs were 67 and 6.75 years, respectively. The mean follow-up is 10.6 months (range: 3 to 17 mo)...
May 8, 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/28486133/rhod-inhibits-rhoc-rock-dependent-cell-contraction-via-pak6
#5
Charlotte H Durkin, Flavia Leite, João V Cordeiro, Yutaka Handa, Yoshiki Arakawa, Ferran Valderrama, Michael Way
RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many different viral infections. We now demonstrate that the vaccinia virus protein F11, which localizes to the plasma membrane, is required for ROCK-mediated cell contraction from 2 hr post infection. Curiously, F11-induced cell contraction is dependent on RhoC and not RhoA signaling to ROCK...
May 8, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28482484/folic-acid-tagged-titanium-dioxide-nanoparticles-for-enhanced-anticancer-effect-in-osteosarcoma-cells
#6
Jin-Wei Ai, Bin Liu, Wei-Dong Liu
In this study, folic acid surface modified-Titanium dioxide nanoparticles (FA-TiNP) were prepared as a suitable alternative to conventional chemotherapeutic agents to treat human osteosarcoma. The particle size of TiNP increased marked after polymer assembly on the nanoparticles (NP) surface with a spherical morphology. FA-TiNP exhibited a superior anticancer effect in osteosarcoma cancer cells compared to that of bare TiNP. The reason might due to the specific interaction of FA with the folate receptor which is overexpressed in the cancer cells...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28481070/-negative-pressure-system-in-the-treatment-of-enterocutaneous-fistulas-in-the-pediatric-population
#7
V Núñez Cerezo, M I Romo Muñoz, M V Amesty Morello, A Vilanova Sánchez, M Dore Reyes, M Gómez Cervantes, A M Andrés Moreno, E Martínez-Ojinaga Nodal, L Martínez Martínez, M López Santamaría
AIM OF THE STUDY: The management of children with enterocutaneous fistulas (EF) along with large abdominal wall defects secondary to multiple surgical interventions can be difficult and sometimes lead to intestinal failure (IF). The aim of this study is to present the results of negative pressure systems and their properties (edema reduction angiogenesis promotion and granulation tissue formation) in children with enterocutaneous fistulas (EF) and their prognosis. MATERIALS AND METHODS: A retrospective analysis of children with refractory, high output EF treated with NPS between 2008-2014...
October 10, 2016: Cirugía Pediátrica: Organo Oficial de la Sociedad Española de Cirugía Pediátrica
https://www.readbyqxmd.com/read/28478671/autonomous-cell-migration-to-csf1-sources-via-a-synthetic-protein-based-system
#8
Anam Qudrat, Kevin Truong
Inflammatory lesions, often seen in diseases such as rheumatoid arthritis, atherosclerosis and cancer, feature an acidic (i.e., low pH) microenvironment rampant with cytokines, such as CSF1. For potential therapeutic intervention targeted at these CSF1 sources, we have assembled a system of four proteins inside a cell (i.e., HEK293) that initially had no natural CSF1-seeking ability. This system included a newly engineered CSF1 chimera receptor (named CSF1Rchi), the previously engineered Ca(2+) activated RhoA (i...
May 10, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28477431/ehrho1-regulates-plasma-membrane-blebbing-through-pi3-kinase-in-entamoeba-histolytica
#9
Ravi Bharadwaj, Ranjana Arya, Sudha Bhattacharya, Alok Bhattacharya
The protozoan parasite Entamoeba histolytica causes amoebiasis, a major public health problem in developing countries. Motility of E. histolytica is important for its pathogenesis. Blebbing is an essential process contributing to cellular motility in many systems. In mammalian cells, formation of plasma membrane blebs is regulated by Rho-GTPases through its effectors, such as ROCK, mDia1 and acto-myosin proteins. In the present study, we have illuminated the role of EhRho1 in bleb formation and motility of E...
May 6, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28475118/effects-of-gelatin-hydrogel-containing-anti-transforming-growth-factor-%C3%AE-antibody-in-a-canine-filtration-surgery-model
#10
Michiko Maeda, Shota Kojima, Tetsuya Sugiyama, Denan Jin, Shinji Takai, Hidehiro Oku, Ryohsuke Kohmoto, Mari Ueki, Tsunehiko Ikeda
In this present study, we investigated the effect of a controlled release of anti-transforming growth factor β (TGF-β) antibody on intraocular pressure (IOP), bleb formation, and conjunctival scarring in a canine glaucoma filtration surgery model using gelatin hydrogel (GH). Glaucoma surgery models were made in 14 eyes of 14 beagles and divided into the following two groups: (1) subconjunctival implantation of anti-TGF-β antibody-loaded GH (GH-TGF-β group, n = 7), and (2) subconjunctival implantation of GH alone (GH group, n = 7)...
May 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28473890/evaluation-of-the-morphological-features-of-the-uterine-tubes-during-postnatal-development-in-west-african-dwarf-goats-capra-hircus
#11
Clifford Nwabugwu Abiaezute, Innocent Chima Nwaogu, Udensi Maduabuchi Igwebuike
The objective of this study was to highlight the postnatal development of the uterine tubes of the West African Dwarf goat from birth to 28 weeks of age by gross examination and light microscopy. There was a caudal migration of the paired uterine tubes from behind the paired kidneys at birth to the pelvic inlet at week 8 of age. Each uterine tube exhibited three segments namely; infundibulum, ampulla and isthmus. A marked flexure, the utero-tubal junction, was the point at which the uterine tubes joined the uterine horns...
2017: Veterinary Research Forum
https://www.readbyqxmd.com/read/28470695/sublethal-oligodendrocyte-injury-a-reversible-condition-in-multiple-sclerosis
#12
Qiao-Ling Cui, Damla Khan, Malena Rone, Vijayaraghava Rao, Radia Marie Johnson, Yun Hsuan Lin, Philippe-Antoine Bilodeau, Jeffery A Hall, Moses Rodriguez, Timothy E Kennedy, Samuel K Ludwin, Jack P Antel
OBJECTIVE: Degeneration of oligodendroglial distal processes has been identified as an early event in MS lesion development. Our objective was to further define the development of the "dying-back" oligodendrocyte lesion in situ and to model the development and potential reversibility of such responses using dissociated cultures of adult human brain-derived oligodendrocytes. Methods In situ analyses were performed on glutaraldehyde-fixed thin sections of clinically acute and pathologically active cases of MS...
May 3, 2017: Annals of Neurology
https://www.readbyqxmd.com/read/28470642/iris-clip-lens-implantation-with-novel-approach-for-aphakia-after-previous-filtration-surgery
#13
A Hammer, T J Wolfensberger
Purpose To present a novel and simplified surgical technique of Artisan lens implantation for intraocular lens luxation and aphakia in glaucoma patients with previous superior filtration surgery. Methods Analysis of 12 patients with a history of previous superior filtration surgery for glaucoma, who underwent artisan lens implantation. To allow lens manipulation and implantation without alteration of the filtration bleb, the main sclerocorneal incision was performed laterally and the Artisan lens was held in place for enclavation of the iris stroma via a superior corneal incision without disturbing the filtration bleb...
April 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28468021/comparison-of-the-outcomes-of-trabeculectomy-with-5-fluorouracil-versus-ologen-implant-in-primary-open-angle-glaucoma
#14
Wisam Shihadeh, Ihab Massad, Yousef Khader, Ahmed Al-Rawi, Tala Alshalakhti
PURPOSE: To compare the outcomes of trabeculectomy with 5-fluorouracil (5-FU) versus Ologen™ implant in patients with primary open-angle glaucoma. METHODS: A retrospective chart review was performed of patients with primary open-angle glaucoma who underwent trabeculectomy using either Ologen or 5-FU over 12 consecutive months. The patients had moderate-to-advanced primary open-angle glaucoma and uncontrolled intraocular pressure (IOP) on maximally tolerated medical treatment...
May 4, 2017: Ophthalmic Research
https://www.readbyqxmd.com/read/28466971/apoptosis-in-acanthamoeba-castellanii-belonging-to-the-t4-genotype
#15
Abdul M Baig, Salima Lalani, Naveed A Khan
Here we describe features of apoptosis in unicellular Acanthamoeba castellanii belonging to the T4 genotype. When exposed to apoptosis-inducing compounds such as doxorubicin, A. castellanii trophozoites exhibited cell shrinkage and membrane blebbing as observed microscopically, DNA fragmentation using agarose gel electrophoresis, and phosphatidylserine (PS) externalization using annexin V immunostaining. Overall, these findings suggest the existence of apoptosis in A. castellanii possibly mediated by intrinsic apoptotic cascade...
May 3, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28465264/quantitative-susceptibility-mapping-as-a-possible-tool-to-radiographically-diagnose-sentinel-headache-associated-with-intracranial-aneurysm-case-report
#16
Daichi Nakagawa, Cameron Cushing, Yasunori Nagahama, Lauren Allan, David Hasan
BACKGROUND: Sentinel headache (SH) is estimated to occur prior to rupture of an aneurysm in 15-60% of aneurysmal subarachnoid hemorrhage (aSAH) cases. Non-contrasted computed tomography (CT) of brain and lumbar puncture are both negative, by definition, in patients presenting with SH. One of the theories explaining this phenomenon is micro-hemorrhage (MH) from the aneurysm wall contributing to iron deposition in the interface between the aneurysm wall and brain parenchyma. Quantitative susceptibility mapping (QSM) is a recently introduced magnetic resonance imaging (MRI) technique that has been shown capable of localizing the deposition of paramagnetic metals, particularly ferric iron...
April 29, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28464893/anticancer-potential-of-thevetia-peruviana-fruit-methanolic-extract
#17
Alberto Ramos-Silva, Faviola Tavares-Carreón, Mario Figueroa, Susana De la Torre-Zavala, Argel Gastelum-Arellanez, Aída Rodríguez-García, Luis J Galán-Wong, Hamlet Avilés-Arnaut
BACKGROUND: Thevetia peruviana (Pers.) K. Schum or Cascabela peruviana (L.) Lippold (commonly known as ayoyote, codo de fraile, lucky nut, or yellow oleander), native to Mexico and Central America, is a medicinal plant used traditionally to cure diseases like ulcers, scabies, hemorrhoids and dissolve tumors. The purpose of this study was to evaluate the cytotoxic, antiproliferative and apoptotic activity of methanolic extract of T. peruviana fruits on human cancer cell lines. METHODS: The cytotoxic activity of T...
May 2, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28464205/endoscopic-management-of-blue-rubber-bleb-nevus-syndrome-in-the-colon-with-hemostatic-clamp-and-snare
#18
Chuncheng Wu, Rong Luo, Xiaoqing Li, Bing Hu
No abstract text is available yet for this article.
May 2, 2017: Endoscopy
https://www.readbyqxmd.com/read/28463800/standardized-polyalthia-longifolia-leaf-extract-plme-inhibits-cell-proliferation-and-promotes-apoptosis-the-anti-cancer-study-with-various-microscopy-methods
#19
Soundararajan Vijayarathna, Yeng Chen, Jagat R Kanwar, Sreenivasan Sasidharan
Over the years a number of microscopy methods have been developed to assess the changes in cells. Some non-invasive techniques such as holographic digital microscopy (HDM), which although does not destroy the cells, but helps to monitor the events that leads to initiation of apoptotic cell death. In this study, the apoptogenic property and the cytotoxic effect of P. longifolia leaf methanolic extract (PLME) against the human cervical carcinoma cells (HeLa) was studied using light microscope (LM), holographic digital microscopy (HDM), scanning electron microscope (SEM) and transmission electron microscope (TEM)...
April 29, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28455543/-early-atrophy-of-the-conjunctiva-after-trabeculectomy-with-mitomycin-c-and-collagen-matrix-implant
#20
R Siggel, T Dietlein
Complete atrophy of the filtration bleb is a very rare complication after trabeculectomy with mitomycin C and collagen matrix implant (Ologen®, Aeon Astron Corporation, Taipei, Taiwan). Clinically, the atrophy can present similar to blebitis. We report the case of a 71-year-old woman, in whom we resected the atrophic conjunctiva and reconstructed a filtration bleb using an amniotic membrane.
April 28, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
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