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Image fusion radis

Guilhem Godlewski, Jacques Gaubert, Renée Cristol-Gaubert, Maïada Radi, Volker Baecker, Pierre Travo, Michel Prudhomme, Dominique Prat-Pradal
AIM: The purpose of the present study was to illustrate the modality of rotation of ventral and dorsal pancreatic buds by three-dimensional (3D) reconstructions in the rat embryos, during the Carnegie stages 13-17. MATERIALS AND METHODS: Serial sections of thirty rat embryos stages 13-17, were observed. The embryos were fixed in Bouin's solution, dehydrated, and paraffin embedded. The sections, 7 μm thick, were cut in longitudinal or transverse planes and were stained alternately by hematoxylin-eosin or Heindenhain' azan...
October 2011: Surgical and Radiologic Anatomy: SRA
M Radi, J Gaubert, R Cristol-Gaubert, V Baecker, P Travo, M Prudhomme, G Godlewski, D Prat-Pradal
AIMS: The goal in this paper was to rebuild a three dimensional (3D) reconstruction of the dorsal and ventral pancreatic buds, in the human embryos, at Carnegie stages 15-23. METHOD: The early development of the pancreas is studied by tissue observation and reconstruction by a computer-assisted method, using a light micrograph images from consecutive serial sagittal sections (diameter 7 microm) of ten human embryos ranging from Carnegie stages 15-23, CRL 7-27 mm, fixed, dehydrated and embedded in paraffin, were stained alternately with haematoxylin-eosin or Heindenhain'Azan...
January 2010: Surgical and Radiologic Anatomy: SRA
J B Cajazeiras, L M Melo, E S Albuquerque, G Rádis-Baptista, B S Cavada, V J F Freitas
Low purification efficiency and incomplete characterization of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His(6) fusion protein in Escherichia coli Top10 cells. Recombinant clones were selected by growth in ampicillin-enriched medium, PCR amplification and nucleotide sequencing. The inserted cDNA was completely identified and recombinant protein synthesis was monitored by SDS-PAGE, followed by immunoblotting with monoclonal anti-His antibody...
2009: Genetics and Molecular Research: GMR
J Gaubert, R Cristol-Gaubert, M Radi, V Baecker, P Travo, M Prudhomme, G Godlewski, D Prat-Pradal
The aim of this work was to reconstruct, in the rat embryos, stage 12-23, the three dimensional (3D) distribution of the dorsal and ventral pancreatic buds by of a computer assisted method. Ninety-six rat embryos, CRL 3-16 mm, fixed, dehydrated, and paraffin embedded, were submitted to serial histological sections and stained by hematoxylin-eosin and Heidenhain's azan techniques. The images were digitalized by Canon Camera 350 EOS D. The serial views were aligned anatomically by software and the data were analyzed following segmentation and thresholding...
January 2009: Surgical and Radiologic Anatomy: SRA
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