keyword
MENU ▼
Read by QxMD icon Read
search

Camelid*

keyword
https://www.readbyqxmd.com/read/28065273/screening-and-characterization-strategies-for-nanobodies-targeting-membrane-proteins
#1
S Veugelen, M Dewilde, B De Strooper, L Chávez-Gutiérrez
The study of membrane protein function and structure requires their successful detection, expression, solubilization, and/or reconstitution, which poses a challenging task and relies on the availability of suitable tools. Several research groups have successfully applied Nanobodies in the purification, as well as the functional and structural characterization of membrane proteins. Nanobodies are small, single-chain antibody fragments originating from camelids presenting on average a longer CDR3 which enables them to bind in cavities and clefts (such as active and allosteric sites)...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28065171/zoonotic-diseases-in-south-american-camelids-in-england-and-wales
#2
K Halsby, D F Twomey, C Featherstone, A Foster, A Walsh, K Hewitt, D Morgan
The number of South American camelids (SACs) in England and Wales is increasing and with this comes a risk of new and emerging infections. Although classified as livestock, these animals are also treated as pets and may be in regular contact with humans. This paper reviews zoonotic diseases that have been identified in SACs in England and Wales, and which pose a potential risk to human health. We also highlight the importance of surveillance continuing to capture information on infections in SACs for the protection of both public and animal health...
January 9, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28043352/current-status-and-future-direction-of-cryopreservation-of-camelid-embryos
#3
REVIEW
M Herrid, G Vajta, J A Skidmore
Over the past 3 decades, and similar to the horse industry, fresh embryo transfer has been widely practiced on large commercial scales in different camelid species, especially the dromedary camel and alpaca. However, the inability to cryopreserve embryos significantly reduces its broader application, and as such limits the capacity to utilize elite genetic resources internationally. In addition, cryopreservation of the semen of camelids is also difficult, suggesting an extreme sensitivity of the germplasm to cooling and freezing...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28012779/vaccination-of-alpacas-against-rift-valley-fever-virus-safety-immunogenicity-and-pathogenicity-of-mp-12-vaccine
#4
M Rissmann, R Ulrich, C Schröder, B Hammerschmidt, D Hanke, C Mroz, M H Groschup, M Eiden
Rift Valley fever (RVF) is an emerging zoonosis of major public health concern in Africa and Arabia. Previous outbreaks attributed camelids a significant role in the epidemiology of Rift Valley fever virus (RVFV), making them an important target species for vaccination. Using three alpacas as model-organisms for dromedary camels, the safety, immunogenicity and pathogenicity of the MP-12 vaccine were evaluated in this study. To compare both acute and subacute effects, animals were euthanized at 3 and 31days post infection (dpi)...
December 21, 2016: Vaccine
https://www.readbyqxmd.com/read/27991956/cord%C3%A3-o-formation-loess-deposits-in-the-southern-coastal-plain-of-the-state-of-rio-grande-do-sul-brazil
#5
Renato P Lopes, Sergio R Dillenburg, Cesar L Schultz
Loess consists of silt-dominated sediments that cover ~10% of the Earth's surface. In southern South America it occurs in Argentina, Bolivia, Paraguay and Uruguay, and its presence in southern Brazil was never studied in detail. Here is proposed a new lithostratigraphic unit, Cordão Formation, consisting of loess deposits in the southern Brazilian coastal plain. It consists of fine-very fine silt with subordinate sand and clay, found mostly in lowland areas between Pleistocene coastal barriers. These sediments are pale-colored (10YR hue) and forms ~1,5-2,0 meter-thick stable vertical walls...
October 2016: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/27965386/active-site-mmp-selective-antibody-inhibitors-discovered-from-convex-paratope-synthetic-libraries
#6
Dong Hyun Nam, Carlos Rodriguez, Albert G Remacle, Alex Y Strongin, Xin Ge
Proteases are frequent pharmacological targets, and their inhibitors are valuable drugs in multiple pathologies. The catalytic mechanism and the active-site fold, however, are largely conserved among the protease classes, making the development of the selective inhibitors exceedingly challenging. In our departure from the conventional strategies, we reviewed the structure of known camelid inhibitory antibodies, which block enzyme activities via their unusually long, convex-shaped paratopes. We synthesized the human Fab antibody library (over 1...
December 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27959623/nanobodies-to-study-g-protein-coupled-receptor-structure-and-function
#7
Aashish Manglik, Brian K Kobilka, Jan Steyaert
Ligand-induced activation of G protein-coupled receptors (GPCRs) is a key mechanism permitting communication between cells and organs. Enormous progress has recently elucidated the structural and dynamic features of GPCR transmembrane signaling. Nanobodies, the recombinant antigen-binding fragments of camelid heavy-chain-only antibodies, have emerged as important research tools to lock GPCRs in particular conformational states. Active-state stabilizing nanobodies have elucidated several agonist-bound structures of hormone-activated GPCRs and have provided insight into the dynamic character of receptors...
January 6, 2017: Annual Review of Pharmacology and Toxicology
https://www.readbyqxmd.com/read/27939542/lama-glama-%C3%AE-s1-casein-identification-of-new-polymorphisms-at-the-csn1s1-gene
#8
A Pauciullo, M Gauly, G Cosenza, H Wagner, G Erhardt
South American camelids have been poorly genetically investigated and little information is available in llamas (Lama glama) regarding the diversity of the caseins at the protein and gene level. Exon skipping and duplication events previously reported in the αS1-casein gene (CSN1S1) led us to investigate the genetic variability at this locus. Seventy-two positive clones for the αS1-casein transcripts were analyzed and randomly sequenced. The comparative analysis of the sequences revealed 2 transitions, c...
December 8, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27929050/first-complete-mitochondrial-genome-data-from-ancient-south-american-camelids-the-mystery-of-the-chilihueques-from-isla-mocha-chile
#9
Michael Westbury, Stefan Prost, Andrea Seelenfreund, José-Miguel Ramírez, Elizabeth A Matisoo-Smith, Michael Knapp
In South American societies, domesticated camelids were of great cultural importance and subject to trade and translocation. South American camelids were even found on remote and hard to reach islands, emphasizing their importance to historic and pre-historic South American populations. Isla Mocha, a volcanic island 35 km offshore of Central-South Chile, is an example of such an island. When Dutch and Spanish explorers reached the island in the early 17th century, they found that domesticated camelids called "chilihueque" played a major role in the island's society...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27905655/-gastrointestinal-bezoars
#10
Ricardo Espinoza González
Gastrointestinal bezoars are a concretion of indigested material that can be found in the gastrointestinal tract of humans and some animals. This material forms an intraluminal mass, more commonly located in the stomach. During a large period of history animal bezoars were considered antidotes to poisons and diseases. We report a historical overview since bezoars stones were thought to have medicinal properties. This magic conception was introduced in South America by Spanish conquerors. In Chile, bezoars are commonly found in a camelid named guanaco (Lama guanicoe)...
August 2016: Revista Médica de Chile
https://www.readbyqxmd.com/read/27903650/structural-analysis-of-single-domain-antibodies-bound-to-a-second-neutralizing-hot-spot-on-ricin-toxin-s-enzymatic-subunit
#11
Michael J Rudolph, David J Vance, Michael S Cassidy, Yinghui Rong, Nicholas J Mantis
Ricin toxin is a heterodimer consisting of RTA, a ribosome-inactivating protein, and RTB, a lectin that facilitates receptor-mediated uptake into mammalian cells. In previous studies, we demonstrated that toxin-neutralizing antibodies target four spatially distinct hotspots on RTA, which we refer to as epitope Clusters I-IV. In this report we identified and characterized three single domain camelid antibodies (VHH) against Cluster II. One of these VHHs, V5E1, ranks as one of the most potent ricin-neutralizing antibodies described to date...
November 30, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27881685/generation-of-camelid-vhh-bispecific-constructs-via-in-cell-intein-mediated-protein-trans-splicing
#12
Yuki Shibuya, Natsuki Haga, Ryutaro Asano, Hikaru Nakazawa, Takamitsu Hattori, Daisuke Takeda, Aruto Sugiyama, Reiko Kurotani, Izumi Kumagai, Mitsuo Umetsu, Koki Makabe
Production of various combinations of bispecific variable domain of heavy chain of heavy chain-only antibody (VHH) constructs to evaluate their therapeutic potential usually requires several gene-engineering steps. Here, we present an alternative method of creating bispecific VHH constructs in vivo through protein trans-splicing (PTS) reaction; this method may reduce the number of gene manipulation steps required. As a proof-of-concept, we constructed a bispecific antibody (bsAb) containing an anti-epidermal growth factor receptor VHH and anti-green fluorescent protein VHH, and we evaluated and confirmed its bispecificity...
January 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/27865111/camelid-and-shark-single-domain-antibodies-structural-features-and-therapeutic-potential
#13
REVIEW
Doreen Könning, Stefan Zielonka, Julius Grzeschik, Martin Empting, Bernhard Valldorf, Simon Krah, Christian Schröter, Carolin Sellmann, Björn Hock, Harald Kolmar
In addition to canonical antibodies composed of heavy and light chains, the adaptive immune systems of camelids and cartilaginous fish comprise heavy-chain only isotypes (HcAb) devoid of light chains, where antigen-binding is mediated exclusively by one variable domain. Due to their inherent favorable attributes, such as high affinity and specificity for their cognate antigen, extraordinary stability, small size and, most importantly, the possibility to complement classical antibodies in terms of 'drugable' target-space, HcAb-derived entities evolved as promising candidates for biomedical applications of which many have already proven to be successful in early stage clinical trials...
November 16, 2016: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/27862476/analysis-of-the-binding-loops-configuration-and-surface-adaptation-of-different-crystallized-single-domain-antibodies-in-response-to-various-antigens
#14
Mohammed M Al Qaraghuli, Valerie A Ferro
Monoclonal antibodies have revolutionized the biomedical field through their ubiquitous utilization in different diagnostics and therapeutic applications. Despite this widespread use, their large size and structural complexity have limited their versatility in specific applications. The antibody variable region that is responsible for binding antigen is embodied within domains that can be rescued individually as single-domain antibody (sdAb) fragments. Because of the unique characteristics of sdAbs, such as low molecular weight, high physicochemical stability, and the ability to bind antigens inaccessible to conventional antibodies, they represent a viable alternative to full-length antibodies...
November 16, 2016: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/27862386/no-detectable-carotenoid-concentrations-in-serum-of-llamas-and-alpacas
#15
J Raila, F J Schweigert, A Stanitznig, B Lambacher, S Franz, S Baldermann, T Wittek
Carotenoids are lipid-soluble pigments and important for a variety of physiological functions. They are major dietary vitamin A precursors and act as lipophilic antioxidants in a variety of tissues and are associated with important health benefits in humans and animals. All animals must acquire carotenoids from their diet, but to our knowledge, there are no studies investigating the intestinal carotenoid absorption and their blood concentrations in New World camelids. The present study aimed to assess the serum concentrations of selected carotenoids in llamas (n = 13) and alpacas (n = 27)...
November 9, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27852863/cryo-electron-microscopy-structures-of-expanded-poliovirus-with-vhhs-sample-the-conformational-repertoire-of-the-expanded-state
#16
Mike Strauss, Lise Schotte, Krishanthi S Karunatilaka, David J Filman, James M Hogle
: By using cryo-electron microscopy, expanded 80S-like poliovirus virions (poliovirions) were visualized in complexes with four 80S-specific camelid VHHs (Nanobodies). In all four complexes, the VHHs bind to a site on the top surface of the capsid protein VP3, which is hidden in the native virus. Interestingly, although the four VHHs bind to the same site, the structures of the expanded virus differ in detail in each complex, suggesting that each of the Nanobodies has sampled a range of low-energy structures available to the expanded virion...
February 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27816596/rapid-capture-and-labeling-of-cells-on-single-domain-antibodies-functionalized-flow-cell
#17
Guan-Yu Chen, Zeyang Li, Joao N Duarte, Alexandre Esteban, Ross W Cheloha, Christopher S Theile, Gerald R Fink, Hidde L Ploegh
Current techniques to characterize leukocyte subgroups in blood require long sample preparation times and sizable sample volumes. A simplified method for leukocyte characterization using smaller blood volumes would thus be useful in diagnostic settings. Here we describe a flow system comprised of two functionalized graphene oxide (GO) surfaces that allow the capture of distinct leukocyte populations from small volumes blood using camelid single-domain antibodyfragments (VHHs) as capture agents. We used site-specifically labeled leukocytes to detect and identify cells exposed to fungal challenge...
March 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27805447/evaluation-of-two-platelet-rich-plasma-processing-methods-and-two-platelet-activation-techniques-for-use-in-llamas-and-alpacas
#18
Stacy A Semevolos, Cori D Youngblood, Stephanie K Grissom, M Elena Gorman, Maureen K Larson
OBJECTIVE To evaluate 2 processing methods (commercial kit vs conical tube centrifugation) for preparing platelet rich plasma (PRP) for use in llamas and alpacas. SAMPLES Blood samples (30 mL each) aseptically collected from 6 healthy llamas and 6 healthy alpacas. PROCEDURES PRP was prepared from blood samples by use of a commercial kit and by double-step conical tube centrifugation. A CBC was performed for blood and PRP samples. Platelets in PRP samples were activated by means of a freeze-thaw method with or without 23mM CaCl2, and concentrations of platelet-derived growth factor-BB and transforming growth factor-β1 were measured...
November 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27791182/crystal-structure-of-a-lacy-nanobody-complex-in-a-periplasmic-open-conformation
#19
Xin Jiang, Irina Smirnova, Vladimir Kasho, Jianping Wu, Kunio Hirata, Meng Ke, Els Pardon, Jan Steyaert, Nieng Yan, H Ronald Kaback
The lactose permease of Escherichia coli (LacY), a dynamic polytopic membrane protein, catalyzes galactoside-H(+) symport and operates by an alternating access mechanism that exhibits multiple conformations, the distribution of which is altered by sugar binding. We have developed single-domain camelid nanobodies (Nbs) against a mutant in an outward (periplasmic)-open conformation to stabilize this state of the protein. Here we describe an X-ray crystal structure of a complex between a double-Trp mutant (Gly46→Trp/Gly262→Trp) and an Nb in which free access to the sugar-binding site from the periplasmic cavity is observed...
November 1, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27762054/ovulation-inducing-factor-oif-ngf-in-seminal-plasma-a-review-and-update
#20
REVIEW
G P Adams, M H Ratto, M E Silva, R A Carrasco
The ovulation-inducing effect of seminal plasma was first reported in Bactrian camels over 30 years ago, and the entity responsible was dubbed 'ovulation-inducing factor' (OIF). More recent studies, primarily in llamas and alpacas, characterized the biological and chemical properties of OIF and ultimately identified it as βNGF. This recent discovery has allowed a convergence of knowledge previously separated by discipline and by mechanism; that is, neurobiology and reproductive biology, and autocrine/paracrine vs endocrine...
October 2016: Reproduction in Domestic Animals, Zuchthygiene
keyword
keyword
173
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"