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Enterovirus 71

Yajie Hu, Jie Song, Longding Liu, Ying Zhang, Lichun Wang, Qihan Li
Enterovirus 71 (EV-A71) and coxsackievirus A16 (CV-A16) remain the predominant etiological agents of hand, foot, and mouth disease (HFMD). The clinical manifestations caused by the two viruses are obviously different. CV-A16 usually triggers a repeated infection, and airway epithelial integrity is often the potential causative factor of respiratory repeated infections. Our previous studies have demonstrated that there were some differentially expressed miRNAs involved in the regulation of adhesion function of epithelial barrier in EV-A71 and CV-A16 infections...
2018: Frontiers in Cellular and Infection Microbiology
Anne J Blaschke, Kristen M Holmberg, Judy A Daly, Amy L Leber, Jennifer Dien Bard, Ernest K Korgenski, Kevin M Bourzac, Kristen J Kanack
In pediatric practice it is common for infants under 2 months of age to undergo evaluation for sepsis when they are ill, often including lumbar puncture (LP) to assess for central nervous system (CNS) infection. The FilmArray® Meningitis/Encephalitis (ME) Panel is a newly approved test for rapid identification of CNS pathogens. Our objective was to study the epidemiology of CNS infection in young infants and the potential impact of rapid multiplex PCR on their care.A performance evaluation of the FilmArray ME Panel was conducted from 2/2014-9/2014 at 11 sites...
April 18, 2018: Journal of Clinical Microbiology
Zhiyong Lou, Yaxin Wang, Jingwei Liu, Lin Cao, Wenjing Wang, Yuna Sun, Zheng Yin
Imaging live virus to monitor the viral entry process is essential to understand virus-host interactions during pathogen infection. However, methods for efficient labeling of live viruses, in particular labeling non-enveloped viruses and tracing virus entry processes, remains limited. Recently, labeling using organometallic palladium reagents has provided a highly efficient and a highly selective way to bioconjugate cysteines of virus proteins. Here, the site-specific bioorthogonal labeling mediated by an organometallic palladium reagent on the surface of live enterovirus-71 (EV71) was used to visualize its entry into live cells...
April 6, 2018: Chembiochem: a European Journal of Chemical Biology
Kun Liu, Yongjin Zhou, Shihan Cui, Jiawen Song, Peipei Ye, Wei Xiang, Xiaoyan Huang, Yiping Chen, Zhihan Yan, Xinjian Ye
BACKGROUND: Brainstem encephalitis is the most common neurologic complication after enterovirus 71 infection. The involvement of brainstem, especially the dorsal medulla oblongata, can cause severe sequelae or death in children with enterovirus 71 infection. We aimed to determine the prevalence of dorsal medulla oblongata involvement in children with enterovirus 71-related brainstem encephalitis (EBE) by using conventional MRI and to evaluate the value of dorsal medulla oblongata involvement in outcome prediction...
April 5, 2018: Pediatric Infectious Disease Journal
Prabuddha S Pathinayake, W A Gayan Chathuranga, Hyun-Cheol Lee, Mohammed Y E Chowdhury, Moon-Hee Sung, Jong-Soo Lee, Chul-Joong Kim
Enterovirus 71 (EV71) is the major causative agent of hand-foot-and-mouth disease (HFMD) and many neurological manifestations. Recently, this virus has become a serious concern because of consecutive epidemics in the Asia-Pacific region. However, no effective vaccine for EV71 has been discovered except two EV71 vaccines which are being used in local communities of China. To develop a safe and efficient EV71 vaccine candidate, we generated inactivated EV71 and evaluated its efficacy with γ-PGA/Chitosan nanoparticles (PC NPs), which are safe, biodegradable and effective as an adjuvant...
April 4, 2018: Archives of Virology
Hongbin Wang, Zheng Li, Junling Niu, Yongfen Xu, Li Ma, Ailing Lu, Xun Wang, Zhikang Qian, Zhong Huang, Xia Jin, Qibin Leng, Jianhua Wang, Jin Zhong, Bing Sun, Guangxun Meng
Iron is an essential nutrient for cell survival and is crucial for DNA replication, mitochondrial function and erythropoiesis. However, the immunological role of iron in viral infections has not been well defined. Here we found the iron salt ferric ammonium citrate (FAC) inhibited Influenza A virus, HIV virus, Zika virus, and Enterovirus 71 (EV71) infections. Of note, both iron ion and citrate ion were required for the antiviral capability of FAC, as other iron salts and citrates did not exhibit viral inhibition...
2018: Cell Discovery
Yingying Ding, Bing Rui, Caixia Gao, Menghua Xu, Lili Wang, Chunyan Zhao, Jie Bai, Jinhong Wang, Jin Xu, Wei Pan
The overall non-neutralizing antibody responses against EV infections among infants and children remain unknown. The non-neutralizing antibody responses against VP1 of EV-A species (Enterovirus 71 (EV71), Coxsackievirus A16 (CA16)), EV-B species (Coxsackievirus B3 (CB3)), EV-C species (Poliovirus 1 (PV1)) and RV-A species (Rhinovirus A N13 (RV13)) were detected and analyzed using a novel evolved immunoglobulin-binding molecule (NEIBM)-based ELISA among infants and children aged 1 day to 6 years in Shanghai...
April 3, 2018: Scientific Reports
Jun Xu, Fang Wang, Desheng Zhao, Jiang Liu, Hong Su, Baolong Wang
Enterovirus 71 (EV71) is the main pathogen of hand-foot-mouth disease (HFMD) and causes several neurological complications. As new strains of EV71 are constantly discovered, it is important to understand the genomic characteristics of the viruses and the mechanism of virulence. Herein, we isolated five strains of EV71 from HFMD patients with or without neurovirulence and sequenced their whole genomes. We then performed whole genome sequence analysis of totally 36 EV71 strains. The phylogenetic analysis of the VP1 region revealed all five isolated strains are clustered into C4a of C4 subgenotype...
March 30, 2018: Journal of Medical Virology
Yuefei Jin, Rongguang Zhang, Weidong Wu, Guangcai Duan
Enterovirus 71 (EV71) infection has become a major threat to global public health, especially in infants and young children. Epidemiological studies have indicated that EV71 infection is responsible for severe and even fatal cases of hand, foot, and mouth disease (HFMD). Accumulated evidence indicates that EV71 infection triggers a plethora of interactive signaling pathways, resulting in host immune evasion and inflammatory response. This review mainly covers the effects of EV71 infection on major antiviral and inflammatory cellular signal pathways...
March 28, 2018: Viruses
Xi Wang, Ke Dong, Min Long, Fang Lin, Zhaowei Gao, Lin Wang, Zhe Zhang, Xi Chen, Ying Dai, Huiping Wang, Huizhong Zhang
Enterovirus 71 (EV71) is the most frequently detected causative agent in hand, foot, and mouth disease (HFMD) and is a serious threat to public health in the Asia-Pacific region. Many EV71 vaccines are under development worldwide, and although both inactivated virus vaccines and virus-like particles (VLPs) are considered to be effective, the main focus has been on inactivated EV71vaccines. There have been very few studies on EV71 VLPs. In this study, using a strategy based on HIV gag VLPs, we constructed a gag-VP1 fusion gene to generate a recombinant baculovirus expressing the EV71 structural protein VP1 together with gag, which was then used to infect TN5 cells to form VLPs...
March 27, 2018: Archives of Virology
Shao-Hua Wang, Ao Wang, Pan-Pan Liu, Wen-Yan Zhang, Juan Du, Shuang Xu, Guan-Chen Liu, Bai-Song Zheng, Chen Huan, Ke Zhao, Xiao-Fang Yu
Coxsackievirus A6 (CV-A6) is an emerging pathogen associated with hand, foot, and mouth disease (HFMD). Its genetic characterization and pathogenic properties are largely unknown. Here, we report 39 circulating CV-A6 strains isolated in 2013 from HFMD patients in Northeast China. Three major clusters of CV-A6 were identified and related to CV-A6 mostly from Shanghai, indicating that domestic CV-A6 strains were responsible for HFMD emerging in Northeast China. Four full-length CV-A6 genomes representing each cluster were sequenced and analyzed further...
March 21, 2018: Journal of Virology
Jingjing Li, Hao Pan, Xiangshi Wang, Qirong Zhu, Yanling Ge, Jiehao Cai, Yuefang Li, Aimei Xia, Jiayu Hu, Mei Zeng
Hand, foot, and mouth disease (HFMD) is mainly epidemic in China and Southeast Asian countries. A novel enterovirus 71 vaccine has been available in China for preventing severe HFMD since 2016. Knowledge of the dynamic epidemiology of HFMD in different regions is necessary for appropriate intervention strategies. This study focused on the citywide surveillance data on the epidemiology and etiology of HFMD in Shanghai during 2014-2016. In these 3 years, the total numbers of reported HFMD cases were 65,018, 39,702, and 57,548, respectively; the numbers of severe cases (case-severity ratios) were 248 (0...
March 21, 2018: Emerging Microbes & Infections
Fei Li, Peipei Liu, Ya Guo, Zhenliang Han, Yedan Liu, Yuanyuan Wang, Long Song, Jianguo Cheng, Zongbo Chen
Enterovirus 71 (EV71) is a single-strand RNA virus that causes hand, foot and mouth disease (HFMD) in infants and young children, leading to neurological complications with significant morbidity and mortality. Unfortunately, the pathogenesis of EV71 infection is not well understood. In this study, we investigated the IL-17F rs1889570 and rs4715290 gene polymorphisms in a Chinese Han population. Severe cases and cases with EV71 encephalitis had a significantly higher frequency of the rs1889570 T/T genotype and T allele...
March 16, 2018: Archives of Virology
Haolong Cong, Ning Du, Yang Yang, Lei Song, Wenliang Zhang, Po Tien
No abstract text is available yet for this article.
April 1, 2018: Journal of Virology
S D Yang, P Q Li, Y G Huang, W Li, L Z Ma, L Wu, N Wang, J M Lu, W Q Chen, Guang-Ming Liu, Y M Xiong, Y L Chen, Ying Zhang
Enterovirus A-71 (EV-A71) may be fatal, but the natural history, symptoms, and signs are poorly understood. This study aimed to examine the natural history of fatal EV-A71 infection and to identify the symptoms and signs of early warning of deterioration. This was a clinical observational study of fatal cases of EV-A71 infection treated at five Chinese hospitals between 1 January 2010 and 31 December 2012. We recorded and analysed 91 manifestations of EV-A71 infection in order to identify early prognosis indicators...
March 12, 2018: Epidemiology and Infection
T Xu, Z Lin, C H Wang, Y Li, M Zhao, L Hua, B Zhu
There is still no effective clinical antiviral drug against human enterovirus 71 (EV71) infection, which causes hand, foot and mouth disease (HFMD) in children. Scavenger receptor class B member 2 (SCARB2) is an important receptor of EV71 as it plays a vital role in the early steps of viral infection. In this study, recombinant SCARB2 protein was expressed and purified in a prokaryotic expression system, and was identified by western blot with a monoclonal antibody and mass spectrometry analysis. Detection of the sera from mice immunized with the recombinant SCARB2 protein using ELISA and western blot showed good immunogenicity of the recombinant protein...
2018: Acta Virologica
Fiona Mei Shan Teo, Min Nyo, Anng Anng Wong, Natalie Woon Hui Tan, Mia Tuang Koh, Yoke Fun Chan, Chia Yin Chong, Justin Jang Hann Chu
Hand, foot and mouth disease (HFMD) is a prevalent contagious childhood disease typically associated with fever, oral lesions and limb exanthema. While HFMD is caused by a plethora of serotypes of viruses under the genus Enterovirus within the Picornaviridae family, Coxsackievirus A16 (CV-A16) and Enterovirus 71 (EV-A71) are considered the main etiological agents. In recent years however, other viruses have also been isolated in considerable numbers from infected individuals in many regions, joining the legion commonly associated with HFMD...
March 6, 2018: Scientific Reports
Caroline J Ross, Ali Rana Atilgan, Özlem Tastan Bishop, Canan Atilgan
Enterovirus 71 can be a severe pathogen in small children and immunocompromised adults. Virus uncoating is a critical step in the infection of the host cell; however, the mechanisms that control this process remain poorly understood. We applied normal mode analysis and perturbation response scanning to several complexes of the virus capsid and present a coarse-graining approach to analyze the full capsid. We show that our method offers an alternative to expressing the system as a set of rigid blocks and accounts for the interconnection between nodes within each subunit and protein interfaces across the capsid...
February 27, 2018: Biophysical Journal
Yuemei Hu, Gang Zeng, Kai Chu, Jing Zhang, Weixiao Han, Ying Zhang, Jing Li, Fengcai Zhu
The longevity of antibodies induced by inactivated enterovirus 71 type (EV71) vaccine is not well studied. To estimate the immunity persistence following two-dose vaccination of EV71 vaccine, a five-year follow-up study was conducted as an extension of a Phase III clinical trial. In this study, a sub-cohort of volunteers who was eligible for enrollment and randomly administrated either 2 dose EV71 vaccine or placebo in the phase III clinical trial was selected, and then further observed 64 months post the 1st vaccination...
February 26, 2018: Human Vaccines & Immunotherapeutics
Ming-Hong Yen, Ching-I Huang, Min-Sheng Lee, Ya-Ping Cheng, Chia Jung Hsieh, Lien-Chai Chiang, Jung-San Chang
Artemisia capillaris (A. capillaris) is a common herbal drug used for thousands years in ancient China. A. capillaris has been empirically used to manage hand-foot-mouth disease (HFMD), which is commonly caused by enterovirus 71 (EV71). EV71 can cause meningoencephalitis with mortality and neurologic sequelae without effective management. It is presently unknown whether A. capillaris is effective against EV71 infection. To test the hypothesis that it could protect cells from EV71-induced injury, a hot water extract of A...
March 2018: Kaohsiung Journal of Medical Sciences
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