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https://www.readbyqxmd.com/read/29449753/do-younger-sleeping-beauties-prefer-a-technological-prince
#1
Anthony F J van Raan, Jos J Winnink
In this paper we investigate recent Sleeping Beauties cited in patents (SB-SNPRs). We find that the increasing trend of the relative number of SBs stopped around 1998. Moreover, we find that the time lag between the publication year of the SB-SNPRs and their first citation in a patent is becoming shorter in recent years. Our observations also suggest that, on average, in the more recent years SBs are awakened increasingly earlier by a 'technological prince' rather than by a 'scientific prince'. These observations suggest that SBs with technological importance are 'discovered' earlier in an application-oriented context...
2018: Scientometrics
https://www.readbyqxmd.com/read/29445422/sleeping-beauty-transposon-integrates-into-non-ta-dinucleotides
#2
Yabin Guo, Yin Zhang, Kaishun Hu
Background: Sleeping Beauty transposon (SB) has become an increasingly important genetic tool for generating mutations in vertebrate cells. It is widely thought that SB exclusively integrates into TA dinucleotides. However, this strict TA-preference has not been rigorously tested in large numbers of insertion sites that now can be detected with next generation sequencing. Li et al. found 71 SB insertions in non-TA dinucleotides in 2013, suggesting that TA dinucleotides are not the only sites of SB integration, yet further studies on this topic have not been carried out...
2018: Mobile DNA
https://www.readbyqxmd.com/read/29444170/genotypic-and-phenotypic-characterization-of-the-sdccag8tn-sb-tyr-2161b-ca1c2ove-mouse-model
#3
Katie Weihbrecht, Wesley A Goar, Calvin S Carter, Val C Sheffield, Seongjin Seo
Nephronophthisis-related ciliopathies (NPHP-RC) are a group of disorders that present with end-stage renal failure in childhood/adolescence, kidney cysts, retinal degeneration, and cerebellar hypoplasia. One disorder that shares clinical features with NPHP-RC is Bardet-Biedl Syndrome (BBS). Serologically defined colon cancer antigen 8 (SDCCAG8; also known as NPHP10 and BBS16) is an NPHP gene that is also associated with BBS. To better understand the patho-mechanisms of NPHP and BBS caused by loss of SDCCAG8 function, we characterized an SDCCAG8 mouse model (Sdccag8Tn(sb-Tyr)2161B...
2018: PloS One
https://www.readbyqxmd.com/read/29443066/constitutive-and-inducible-systems-for-genetic-in-vivo-modification-of-mouse-hepatocytes-using-hydrodynamic-tail-vein-injection
#4
Eric K Hubner, Christian Lechler, Thomas N Rösner, Birgit Kohnke-Ertel, Roland M Schmid, Ursula Ehmer
In research models of liver cancer, regeneration, inflammation, and fibrosis, flexible systems for in vivo gene expression and silencing are highly useful. Hydrodynamic tail vein injection of transposon-based constructs is an efficient method for genetic manipulation of hepatocytes in adult mice. In addition to constitutive transgene expression, this system can be used for more advanced applications, such as shRNA-mediated gene knock-down, implication of the CRISPR/Cas9 system to induce gene mutations, or inducible systems...
February 2, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29432776/establishment-of-cre-mediated-hbv-recombinant-cccdna-rcccdna-cell-line-for-cccdna-biology-and-antiviral-screening-assays
#5
Min Wu, Jin Li, Lei Yue, Lu Bai, Yaming Li, Jieliang Chen, Xiaonan Zhang, Zhenghong Yuan
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA), existing in hepatocyte nuclei as a stable minichromosome, plays a central role in the life cycle of the virus and permits the persistence of infection. Despite being essential for HBV infection, little is known about the molecular mechanisms of cccDNA formation, regulation and degradation, and there is no therapeutic agents directly targeting cccDNA, fore mostly due to the lack of robust, reliable and quantifiable HBV cccDNA models. In this study, combined the Cre/loxP and sleeping beauty transposons system, we established HepG2-derived cell lines integrated with 2-60 copies of monomeric HBV genome flanked by loxP sites (HepG2-HBV/loxP)...
February 9, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29425968/characterization-of-calcium-signals-in-human-induced-pluripotent-stem-cell-derived-dentate-gyrus-neuronal-progenitors-and-mature-neurons-stably-expressing-an-advanced-calcium-indicator-protein
#6
Gergő Vőfély, Tünde Berecz, Eszter Szabó, Kornélia Szebényi, Edit Hathy, Tamás I Orbán, Balázs Sarkadi, László Homolya, Maria C Marchetto, János M Réthelyi, Ágota Apáti
Pluripotent stem cell derived human neuronal progenitor cells (hPSC-NPCs) and their mature neuronal cell culture derivatives may efficiently be used for central nervous system (CNS) drug screening, including the investigation of ligand-induced calcium signalization. We have established hippocampal NPC cultures derived from human induced PSCs, which were previously generated by non-integrating Sendai virus reprogramming. Using established protocols these NPCs were differentiated into hippocampal dentate gyrus neurons...
February 6, 2018: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/29409568/the-effect-of-selective-c-met-inhibitor-on-hcc-in-the-met-active-%C3%AE-catenin-mutated-mouse-model
#7
Na Zhan, Adeola Adebayo Michael, Kaiyuan Wu, Gang Zeng, Aaron Bell, Junyan Tao, Satdarshan P Monga
Simultaneous mutations in CTNNB1 and activation of c-MET occurs in 9-12.5% of patients with hepatocellular cancer (HCC). Co-expression of c-MET-V5 and mutant-β-catenin-Myc in mouse liver by sleeping beauty transposon/transposase and hydrodynamic tail vein injection (SB-HTVI) led to development of HCC with 70% molecular identity to the clinical subset. Using this model, we investigated the effect of EMD1214063, a highly selective c-MET inhibitor. Five weeks after SB-HTVI when tumors were established, EMD1214063 (10mg/kg) was administered by gastric lavage as a single-agent on 5-days-on/3-days-off schedule, compared to vehicle only control...
February 6, 2018: Gene Expression
https://www.readbyqxmd.com/read/29405533/the-role-of-sleep-in-aesthetic-perception-and-empathy-a-mediation-analysis
#8
Sara Peretti, Daniela Tempesta, Valentina Socci, Maria C Pino, Monica Mazza, Marco Valenti, Luigi De Gennaro, Cinzia Di Dio, Antonella Marchetti, Michele Ferrara
The ability to experience aesthetics plays a fundamental role in human social interactions, as well as the capacity to feel empathy. Some studies have shown that beauty perception shares part of the neural network underlying emotional and empathic abilities, which are also known to affect sleep quality and duration. In this study, we evaluated for the first time the effects of sleep on the relation between aesthetic perception and empathic abilities in healthy subjects using a mediation analysis approach. One-hundred and twenty-six subjects participated in this study...
February 6, 2018: Journal of Sleep Research
https://www.readbyqxmd.com/read/29364270/an-efficient-in-vitro-transposition-method-by-a-transcriptionally-regulated-sleeping-beauty-system-packaged-into-an-integration-defective-lentiviral-vector
#9
Daniela Benati, Fabienne Cocchiarella, Alessandra Recchia
The Sleeping Beauty (SB) transposon is a non-viral integrating system with proven efficacy for gene transfer and functional genomics. To optimize the SB transposon machinery, a transcriptionally regulated hyperactive transposase (SB100X) and T2-based transposon are employed. Typically, the transposase and transposon are provided transiently by plasmid transfection and SB100X expression is driven by a constitutive promoter. Here, we describe an efficient method to deliver the SB components to human cells that are resistant to several physical and chemical transfection methods, to control SB100X expression and stably integrate a gene of interest (GOI) through a "cut and paste" SB mechanism...
January 12, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29362220/integration-of-oncogenes-via-sleeping-beauty-as-a-mouse-model-of-hpv16-oral-tumor-and-immunologic-control
#10
Yi-Hsin Lin, Ming-Chieh Yang, Ssu-Hsueh Tseng, Rosie Jiang, Andrew Yang, Emily Farmer, Shiwen Peng, Talia Henkle, Yung-Nien Chang, Chien-Fu Hung, T-C Wu
Human papillomavirus type 16 (HPV16) is the etiologic factor for cervical cancer and a subset of oropharyngeal cancers. Although several prophylactic HPV vaccines are available, no effective therapeutic strategies to control active HPV diseases exist. Tumor implantation models are traditionally used to study HPV-associated buccal tumors. However, they fail to address precancerous phases of disease progression and display tumor microenvironments distinct from those observed in patients. Previously, K14-E6/E7 transgenic mouse models have been used to generate spontaneous tumors...
January 23, 2018: Cancer Immunology Research
https://www.readbyqxmd.com/read/29356866/retrofitting-baculoviral-vector-with-sleeping-beauty-transposon-system-competent-for-long-term-reporter-gene-imaging-in-vivo
#11
Yu Pan, Jing Lv, Donghui Pan, Min Yang, Huijun Ju, Jinxin Zhou, Liying Zhu, Yifan Zhang
Reporter gene imaging is widely used for non-invasively detecting tumorigenesis, trafficking therapeutic cells, and monitoring treatment effect. Baculoviral vectors (BVs) have been utilized as transgenic vectors in the reporter gene imaging systems in recent years. However, BV-mediated report gene imaging can only provide short-term investigation due to its transient transgene expression, which is incompetent for the long-term applications. In the current study, we reconstructed a series of hybrid BVs with several elements, to investigate the feasibility of this hybrid BV-mediated long-term reporter gene imaging in vivo...
January 22, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29288565/high-and-long-term-von-willebrand-factor-expression-after-sleeping-beauty-transposon-mediated-gene-therapy-in-a-mouse-model-of-severe-von-willebrand-disease
#12
Irina Portier, Karen Vanhoorelbeke, Sebastien Verhenne, Inge Pareyn, Nele Vandeputte, Hans Deckmyn, Daniel S Goldenberg, Himanshu B Samal, Manvendra Singh, Zoltán Ivics, Zsuzsanna Izsvák, Simon F De Meyer
BACKGROUND: Type 3 von Willebrand disease (VWD) is characterized by complete absence of von Willebrand factor (VWF). Current therapy is limited to treatment with exogenous VWF/FVIII products, which only provide a short-term solution. Gene therapy offers the potential for a long-term treatment for VWD. OBJECTIVES: To develop an integrative Sleeping Beauty (SB) transposon-mediated VWF gene transfer approach in a preclinical mouse model of severe VWD. METHODS: We established a robust platform for sustained transgene murine (m)VWF expression in the liver of Vwf-/- mice by combining a liver-specific promoter with a sandwich transposon design and the SB100X transposase via hydrodynamic gene delivery...
December 29, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29246287/long-term-pedf-release-in-rat-iris-and-retinal-epithelial-cells-after-sleeping-beauty-transposon-mediated-gene-delivery
#13
Laura Garcia-Garcia, Sergio Recalde, Maria Hernandez, Jaione Bezunartea, Juan Roberto Rodriguez-Madoz, Sandra Johnen, Sabine Diarra, Corinne Marie, Zsuzsanna Izsvák, Zoltán Ivics, Daniel Scherman, Martina Kropp, Gabriele Thumann, Felipe Prosper, Patricia Fernandez-Robredo, Alfredo Garcia-Layana
Pigment epithelium derived factor (PEDF) is a potent antiangiogenic, neurotrophic, and neuroprotective molecule that is the endogenous inhibitor of vascular endothelial growth factor (VEGF) in the retina. An ex vivo gene therapy approach based on transgenic overexpression of PEDF in the eye is assumed to rebalance the angiogenic-antiangiogenic milieu of the retina, resulting in growth regression of choroidal blood vessels, the hallmark of neovascular age-related macular degeneration. Here, we show that rat pigment epithelial cells can be efficiently transfected with the PEDF-expressing non-viral hyperactive Sleeping Beauty transposon system delivered in a form free of antibiotic resistance marker miniplasmids...
December 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/29240762/rethinking-3r-strategies-digging-deeper-into-animaltestinfo-promotes-transparency-in-in-vivo-biomedical-research
#14
Bettina Bert, Antje Dörendahl, Nora Leich, Julia Vietze, Matthias Steinfath, Justyna Chmielewska, Andreas Hensel, Barbara Grune, Gilbert Schönfelder
In the European Union (EU), animal welfare is seen as a matter of great importance. However, with respect to animal experimentation, European citizens feel quite uninformed. The European Directive 2010/63/EU for the protection of laboratory animals aims for greater transparency and requires that a comprehensible, nontechnical summary (NTS) of each authorised research project involving animals is published by the respective Member State. However, the NTSs remain sleeping beauties if their contents are not easily and systematically accessible...
December 2017: PLoS Biology
https://www.readbyqxmd.com/read/29218067/perceived-age-and-life-style-the-specific-contributions-of-seven-factors-involved-in-health-and-beauty
#15
Victor Gabriel Clatici, Daniel Racoceanu, Claude Dalle, Cristiana Voicu, Lucia Tomas-Aragones, Servando E Marron, Uwe Wollina, Simona Fica
OBJECTIVES: The skin is a dynamic, visible organ, showing the most obvious signs of aging. The mechanisms of extrinsic aging, most of them presented in this paper, are currently well known and also the only ones that can be counteracted. Therefore, the transition of this knowledge in the general population is of the most importance, in order to introduce healthy aging strategies, to prevent the development of chronic or malignant diseases and psychological burden related to old age. MATERIALS AND METHODS: A thorough review of the literature has been performed in order to identify the main factors involved in skin health and aging...
September 2017: Mædica
https://www.readbyqxmd.com/read/29130932/p120-catenin-is-an-obligate-haploinsufficient-tumor-suppressor-in-intestinal-neoplasia
#16
Sarah P Short, Jumpei Kondo, Whitney G Smalley-Freed, Haruna Takeda, Michael R Dohn, Anne E Powell, Robert H Carnahan, Mary K Washington, Manish Tripathi, D Michael Payne, Nancy A Jenkins, Neal G Copeland, Robert J Coffey, Albert B Reynolds
p120-Catenin (p120) functions as a tumor suppressor in intestinal cancer, but the mechanism is unclear. Here, using conditional p120 knockout in Apc-sensitized mouse models of intestinal cancer, we have identified p120 as an "obligatory" haploinsufficient tumor suppressor. Whereas monoallelic loss of p120 was associated with a significant increase in tumor multiplicity, loss of both alleles was never observed in tumors from these mice. Moreover, forced ablation of the second allele did not further enhance tumorigenesis, but instead induced synthetic lethality in combination with Apc loss of heterozygosity...
November 13, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29089466/preclinical-and-clinical-advances-in-transposon-based-gene-therapy
#17
Jaitip Tipanee, Yoke Chin Chai, Thierry VandenDriessche, Marinee K Chuah
Transposons derived from Sleeping Beauty (SB), piggyBac (PB) or Tol2 typically require cotransfection of transposon DNA with a transposase either as an expression plasmid or mRNA. Consequently, this results in genomic integration of the potentially therapeutic gene into chromosomes of the desired target cells and thus conferring stable expression. Nonviral transfection methods are typically preferred to deliver the transposon components in to the target cells. However, these methods do not match the efficacy typically attained with viral vectors and are sometimes associated with cellular toxicity evoked by the DNA itself...
October 31, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29077768/secretion-of-a-recombinant-protein-without-a-signal-peptide-by-the-exocrine-glands-of-transgenic-rabbits
#18
Andrea Kerekes, Orsolya Ivett Hoffmann, Gergely Iski, Nándor Lipták, Elen Gócza, Wilfried A Kues, Zsuzsanna Bősze, László Hiripi
Transgenic rabbits carrying mammary gland specific gene constructs are extensively used for excreting recombinant proteins into the milk. Here, we report refined phenotyping of previously generated Venus transposon-carrying transgenic rabbits with particular emphasis on the secretion of the reporter protein by exocrine glands, such as mammary, salivary, tear and seminal glands. The Sleeping Beauty (SB) transposon transgenic construct contains the Venus fluorophore cDNA, but without a signal peptide for the secretory pathway, driven by the ubiquitous CAGGS (CAG) promoter...
2017: PloS One
https://www.readbyqxmd.com/read/29059366/sbcddb-sleeping-beauty-cancer-driver-database-for-gene-discovery-in-mouse-models-of-human-cancers
#19
Justin Y Newberg, Karen M Mann, Michael B Mann, Nancy A Jenkins, Neal G Copeland
Large-scale oncogenomic studies have identified few frequently mutated cancer drivers and hundreds of infrequently mutated drivers. Defining the biological context for rare driving events is fundamentally important to increasing our understanding of the druggable pathways in cancer. Sleeping Beauty (SB) insertional mutagenesis is a powerful gene discovery tool used to model human cancers in mice. Our lab and others have published a number of studies that identify cancer drivers from these models using various statistical and computational approaches...
October 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29037366/oral-tolerance-induction-in-allergy-kissing-awake-a-sleeping-beauty
#20
EDITORIAL
Rudolf Valenta
No abstract text is available yet for this article.
April 2017: Seminars in Immunology
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