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https://www.readbyqxmd.com/read/30336241/fabrication-of-a-glycation-induced-amyloid-nanofibril-and-polyalizarin-yellow-r-nanobiocomposite-application-for-electrocatalytic-determination-of-hydrogen-peroxide
#1
Nader Amini, Mohammad Bagher Gholivand, Mojtaba Shamsipur, Ali Akbar Moosavi Movahedi, Sara Farahi, Mehran Habibi-Rezaei, Afshin Maleki, Reza Rezaee, Kazhal Naderi
Amyloid fibrils were produced in a solution of bovine serum albumin (BSA) in buffer solution in the presence of fructose. The solution was incubated for 20 weeks in the dark. We used glycation induced bovine serum albumin in which fibrilogenesis (nano fibrils) followed by using fluorescence (Thioflavin T), Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) to achieve the size and morphology of fibrils. A novel electrochemical biosensor for the detection of hydrogen peroxide was developed based on immobilizing poly (alizarin yellow R) and amyloid nano-fibrils on glassy carbon electrode (PAYR/AMLNFibs/GCE)...
October 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/30321785/surface-plasmon-resonance-based-indirect-immunoassay-for-detection-of-17%C3%AE-estradiol
#2
Sunita Kumbhat, Rakhee Gehlot, Kavita Sharma, Urvasini Singh, Vinod Joshi
A surface plasmon resonance (SPR) based immunosensor is presented for highly sensitive and selective detection of 17β-estradiol by the indirect competitive inhibition immuno assay, employing anti-17 β-estradiol antibody as high molecular weight (HMW) interactant. Immobilization of estradiol-BSA conjugate onto the nano thin gold surface was accomplished by covalent amide linkage through self assembled monolayer. The proposed biosensor is simple to fabricate, reproducible and exhibit excellent sensitivity for estrogen (detection limit,1 pg mL-1 ) without any significant interference from structurally similar steroidal hormone, progesterone and non-steroidal compound bisphenol-A...
October 9, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30289988/dielectrophoretic-manipulation-of-nanomaterials-a-review
#3
REVIEW
Linbo Liu, Ke Chen, Nan Xiang, Zhonghua Ni
Nanomaterials manipulation using dielectrophoresis (DEP) is one of the major research areas that could potentially benefit the micro/nano science for diverse applications, such as microfluidics, nanomachine, and biosensor. The innovation and development of basic theories, methods or applications will have a huge impact on the entire related field. Specifically, for DEP manipulation of nanomaterials, improvements in comprehensive performance of accuracy, flexibility and scale could promote broader applications in micro/nano science...
October 5, 2018: Electrophoresis
https://www.readbyqxmd.com/read/30287746/functionalized-gold-nanoparticles-as-biosensors-for-monitoring-cellular-uptake-and-localization-in-normal-and-tumor-prostatic-cells
#4
Marianna Pannico, Anna Calarco, Gianfranco Peluso, Pellegrino Musto
In the present contribution the fabrication and characterization of functionalized gold nanospheres of uniform shape and controlled size is reported. These nano-objects are intended to be used as Surface Enhanced Raman Spectroscopy (SERS) sensors for in-vitro cellular uptake and localization. Thiophenol was used as molecular reporter and was bound to the Au surface by a chemisorption process in aqueous solution. The obtained colloidal solution was highly stable and no aggregation of the single nanospheres into larger clusters was observed...
October 4, 2018: Biosensors
https://www.readbyqxmd.com/read/30275947/optical-conductivity-based-ultrasensitive-mid-infrared-biosensing-on-a-hybrid-metasurface
#5
Yibo Zhu, Zhaoyi Li, Zhuang Hao, Christopher DiMarco, Panita Maturavongsadit, Yufeng Hao, Ming Lu, Aaron Stein, Qian Wang, James Hone, Nanfang Yu, Qiao Lin
Optical devices are highly attractive for biosensing as they can not only enable quantitative measurements of analytes but also provide information on molecular structures. Unfortunately, typical refractive index-based optical sensors do not have sufficient sensitivity to probe the binding of low-molecular-weight analytes. Non-optical devices such as field-effect transistors can be more sensitive but do not offer some of the significant features of optical devices, particularly molecular fingerprinting. We present optical conductivity-based mid-infrared (mid-IR) biosensors that allow for sensitive and quantitative measurements of low-molecular-weight analytes as well as the enhancement of spectral fingerprints...
2018: Light, Science & Applications
https://www.readbyqxmd.com/read/30261251/graphene-and-graphene-oxide-functionalization-and-nano-bio-catalytic-system-for-enzyme-immobilization-and-biotechnological-perspective
#6
REVIEW
Muhammad Adeel, Muhammad Bilal, Tahir Rasheed, Ashutosh Sharma, Hafiz M N Iqbal
Graphene-based nanomaterials have gained high research interest in different fields related to proteins and thus are rapidly becoming the most widely investigated carbon-based materials. Their exceptional physiochemical properties such as electrical, optical, thermal and mechanical strength enable graphene to render graphene-based nanostructured materials suitable for applications in different fields such as electroanalytical chemistry, electrochemical sensors and immobilization of biomolecules and enzymes...
September 24, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/30259729/hydrodynamic-layer-by-layer-assembly-of-transferrable-enzymatic-conductive-nano-networks-for-enzyme-sticker-based-contact-printing-of-electrochemical-biosensors
#7
Seung-Woo Lee, Tae-Hyung Kang, Sung Ku Lee, Ki-Young Lee, Hyunjung Yi
Realizing high-performance electrochemical biosensors in a simple contact-printing-based approach significantly increases the applicability of integrated flexible biosensors. Herein, an enzyme-sticker-based approach that enables flexible and multi electrochemical sensors via simple contact-transfer-printing is reported. The enzyme sticker consists of an enzymatic conductive network film and a polymeric support. The enzyme-incorporated nanostructured conductive network showing efficient electrical coupling was assembled via hydrodynamic layer-by-layer assembly of redox enzymes, polyelectrolytes, single-walled carbon nanotubes and biological glue material, M13 phage...
September 27, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30254832/high-throughput-micro-nanostructuring-by-microdroplet-inkjet-printing
#8
Hendrikje R Neumann, Christine Selhuber-Unkel
The production of micrometer-sized structures comprised of nanoparticles in defined patterns and densities is highly important in many fields, ranging from nano-optics to biosensor technologies and biomaterials. A well-established method to fabricate quasi-hexagonal patterns of metal nanoparticles is block copolymer micelle nanolithography, which relies on the self-assembly of metal-loaded micelles on surfaces by a dip-coating or spin-coating process. Using this method, the spacing of the nanoparticles is controlled by the size of the micelles and by the coating conditions...
2018: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/30243666/binary-colloidal-crystals-bccs-interactions-fabrication-and-applications
#9
REVIEW
Farhana Sharmin Diba, Andrew Boden, Helmut Thissen, Mrinal Bhave, Peter Kingshott, Peng-Yuan Wang
The organization of matter into hierarchical structures is a fundamental characteristic of functional materials and living organisms. Binary colloidal crystal (BCC) systems present a diversified range of nanotopographic structures where large and small colloidal particles simultaneously self-assemble into either 2D monolayer or 3D hierarchical crystal lattices. More importantly, understanding how BCCs form opens up the possibility to fabricate more complex systems such as ternary or quaternary colloidal crystals...
August 23, 2018: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/30227710/cosensitization-strategy-with-cascade-energy-level-arrangement-for-ultrasensitive-photoelectrochemical-protein-detection
#10
Yan-Hui Zhang, Ying-Ning Zheng, Meng-Jie Li, Tao Hu, Ruo Yuan, Sha-Ping Wei
Here, a photoelectrochemical (PEC) biosensor was established by a cosensitization strategy with cascade energy level arrangement for ultrasensitive detection of prostate-specific antigen (PSA). The proposed cosensitization strategy was based on the well-matched energy level arrangement of four kinds of organic photoactive materials, in which poly{4,8-bis[5-(2-ethylhexyl)thiophen-2-yl]benzo[1,2- b:4,5- b']dithiophene-2,6-diyl- alt-3-fluoro-2-[(2-ethylhexyl)-carbonyl]thieno[3,4- b]thiophene-4,6-diyl} (PTB7-Th) was used as the photoactive material and perylenetetracarboxyl diimide (PDI), fullerene (nano-C60 ), and polyaniline (PANI) were employed as the sensitizers...
October 3, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30219672/an-electrochemical-biosensor-to-distinguish-between-normal-and-cancer-cells-based-on-monitoring-their-acidosis-using-gold-coated-silicon-nano-roughened-electrode
#11
Alireza Alikhani, Milad Gharooni, Hassan Moghtaderi, Fatemeh Farokhmanesh, Hamed Abiri, Mona Salimi, Farnoosh Attari, Mohammad Abdolahad
One of the most interesting fields of research in cancer diagnosis is tracing the relation between extracellular media and cancer progression. Detecting the secreting contents of the cells and translating these molecular identifications into label-free recognizable patterns would open new opportunities in cancer research. Electrochemical responses are in the range of most attractive sensing mechanisms especially in biochemical approaches. Perturbed ionic exchanges as a known biochemical function of cancer cells presented a strong correlation with the pH of the tumor microenvironment...
November 15, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/30218125/graphene-quantum-dots-based-nano-biointerface-platform-for-food-toxin-detection
#12
Hema Bhardwaj, Chandan Singh, R K Kotnala, Gajjala Sumana
Due to the similar electrochemical properties to graphene oxide (GO), graphene quantum dots (GQDs) are considered as a highly potential candidate for designing an electrochemical biosensor. In this report, GQDs were synthesized having an average diameter of 7 nm and utilized for the fabrication of an electrochemical immunosensor for the detection of food toxin, aflatoxin B1 (AFB1 ). An electrophoretic deposition technique was utilized to deposit the chemically synthesized GQDs onto indium tin oxide (ITO)-coated glass substrate...
September 14, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30218124/toehold-integrated-molecular-beacon-system-for-a-versatile-non-enzymatic-application
#13
Zhenping Liu, Yiyun Wang, Xuchu Wang, Weiwei Liu, Yibei Dai, Pan Yu, Zhaoping Liao, Ying Ping, Zhihua Tao
A molecular beacon (MB) is an oligonucleotide hybridization probe with a hairpin-shaped structure that leads to specific and instantaneous nucleic acid hybridization, enabling a variety of applications. However, integration of additional module sequences interferes with the performance of MBs and increases the complexity of sequence design. Herein, we develop and characterize a toehold integrated molecular beacon (ToMB) strategy for nucleic acid hybridization, where the reaction rate can be flexibly regulated by a target-induced MB conformational switch...
September 14, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30174274/multiplex-measurement-of-twelve-tumor-markers-using-a-gmr-multi-biomarker-immunoassay-biosensor
#14
Yang Gao, Weisong Huo, Lei Zhang, Jie Lian, Wei Tao, Chuan Song, Jinping Tang, Stone Shi, Yunhua Gao
Tumor markers play an important role in the early diagnosis and therapeutic effect monitoring of tumors. Combined detection of multiple tumor markers is a realistic way of improving the sensitivity and specificity of cancer diagnosis. To achieve this, we studied and designed a giant magneto resistance (GMR) multi-biomarker immunoassay biosensor that can simultaneously detect twelve kinds of tumor markers by integrating a GMR sensor chip, a microfluidic device, a magnetic nano-beads label, and a double antibody sandwich immunoassay method...
August 25, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30172235/label-free-ultrasensitive-detection-of-breast-cancer-mirna-21-biomarker-employing-electrochemical-nano-genosensor-based-on-sandwiched-agnps-in-pani-and-n-doped-graphene
#15
Razieh Salahandish, Ali Ghaffarinejad, Eskandar Omidinia, Hossein Zargartalebi, Keivan Majidzadeh-A, Seyed Morteza Naghib, Amir Sanati-Nezhad
MicroRNAs (miRNAs) are small, endogenous, noncoding RNAs, shown to be expressed abnormally in many tumors and identified as predictive biomarkers for early diagnosis of several cancers including the breast. Therefore, the label-free and highly sensitive detection of miRNAs is of critical significance. In this work, a highly sensitive and label-free nano-genosensor is developed for the detection of miRNA-21, a known breast cancer biomarker, based on a specific architecture of nitrogen-doped functionalized graphene (NFG), silver nanoparticles (AgNPs), and polyaniline (PANI) that resulted in a remarkable effect on signal amplification...
November 30, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30159687/a-novel-magnetoelastic-immunosensor-for-ultrasensitively-detecting-carcinoembryonic-antigen
#16
Yixia Zhang, Xing Guo, Lu Fan, Qiang Zhang, Shengbo Sang
A novel wireless immunosensor is developed for the ultra-sensitive detection of carcinoembryonic antigen. The optimum dimension of the microchips, as magnetoelastic sensitive units, was evaluated by simulation and experiments. The unique effects signal amplification and biocompatibility of gold particles contribute to the stability and sensitivity of the sensor. Furthermore, to enhance sensitivity, the working concentrations of antibody and BSA are selected to be 50 mg/mL and 0.1%, respectively. Atom force microscope imaging sheds light on the biological analysis...
August 29, 2018: Nanoscale Research Letters
https://www.readbyqxmd.com/read/30140055/facile-and-inexpensive-fabrication-of-zinc-oxide-based-bio-surfaces-for-c-reactive-protein-detection
#17
Lu Cao, Janice Kiely, Martina Piano, Richard Luxton
The paper reports a biosensor formed from antibody coated ZnO nano-crystals which has been prepared using a rapid and inexpensive fabrication method which utilises colloidal dispersion enhanced using sonication. This technique was used to prepare highly ordered and uniform nano-crystalline sensor surfaces on polyethylene terephthalate (PET) using 0.5%, 1% and 5% concentrations of zinc oxide nano-crystal suspensions. Impedance spectroscopy was employed to interrogate the sensor surfaces and confirmed high reproducibility of the fabrication process...
August 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30139622/a-new-coccolith-modified-electrode-based-biosensor-using-a-cognate-pair-of-aptamers-with-sandwich-type-binding
#18
Sang Hoon Kim, Onyou Nam, EonSeon Jin, Man Bock Gu
In this study, we report a cognate pair of the aptamer-based sandwich-type electrochemical biosensor for type 2 diabetes biomarker (Vaspin) using coccolith modified electrodeposited on the screen-printed gold electrode (CME-SPGE). The coccolith derived from E. huxleyi used in this study were known to be highly-structured microparticles with many nano-sized pores. The CME-SPGE was successfully fabricated by drop-casting coccoliths, followed by Au sputtering and electrodeposition of Au. On this CME-SPGE electrode, the sandwich-type electrochemical aptasensor was fabricated by using a cognate pair of aptamers...
August 11, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30138342/dynamic-inking-of-large-scale-stamps-for-multiplexed-microcontact-printing-and-fabrication-of-cell-microarrays
#19
Julie Foncy, Aurore Estève, Amélie Degache, Camille Colin, Xavier Dollat, Jean-Christophe Cau, Christophe Vieu, Emmanuelle Trévisiol, Laurent Malaquin
Microcontact printing has become a versatile soft lithography technique used to produce molecular micro- and nano-patterns consisting of a large range of different biomolecules. Despite intensive research over the last decade and numerous applications in the fields of biosensors, microarrays and biomedical applications, the large-scale implementation of microcontact printing is still an issue. It is hindered by the stamp-inking step that is critical to ensure a reproducible and uniform transfer of inked molecules over large areas...
2018: PloS One
https://www.readbyqxmd.com/read/30114919/scalable-and-tunable-periodic-graphene-nanohole-arrays-for-mid-infrared-plasmonics
#20
Kavitha K Gopalan, Bruno Paulillo, David M A Mackenzie, Daniel Rodrigo, Nestor Bareza, Patrick R Whelan, Abhay Shivayogimath, Valerio Pruneri
Despite its great potential for a wide variety of devices, especially mid-infrared biosensors and photodetectors, graphene plasmonics is still confined to academic research. A major reason is the fact that, so far, expensive and low-throughput lithography techniques are needed to fabricate graphene nanostructures. Here, we report for the first time a detailed experimental study on electrostatically tunable graphene nanohole array surfaces with periods down to 100 nm, showing clear plasmonic response in the range ∼1300-1600 cm-1 , which can be fabricated by a scalable nanoimprint technique...
August 24, 2018: Nano Letters
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