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Nano Biosensor

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https://www.readbyqxmd.com/read/30087383/in-situ-viscoelastic-properties-and-chain-conformations-of-heavily-hydrated-carboxymethyl-dextran-layers-a-comparative-study-using-owls-and-qcm-i-chips-coated-with-waveguide-material
#1
Andras Saftics, György Aurél Prósz, Barbara Türk, Beatrix Peter, Sándor Kurunczi, Robert Horvath
Hydration, viscoelastic properties and dominant structure of thin polymer layers on the surface of waveguide material were evaluated using optical waveguide lightmode spectroscopy (OWLS) and quartz crystal microbalance (QCM) methods. The fundamentally different principles of the two applied label-free biosensors enable to examine analyte layers from complementary aspects, e.g. to determine the amount of bound water in hydrated layers. In this study, a new QCM instrument with impedance measurement (QCM-I) is introduced...
August 7, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30086979/facile-preparation-of-bright-orange-fluorescent-carbon-dots-and-the-constructed-biosensing-platform-for-the-detection-of-ph-in-living-cells
#2
Yuan-Yuan Ding, Xiao-Juan Gong, Yang Liu, Wen-Jing Lu, Yi-Fang Gao, Ming Xian, Shao-Min Shuang, Chuan Dong
Long wavelength (i.e., orange- to red-light) fluorescence emission and functionality are critically longing for the development and applications of carbon dots (CDs) toward biosensor and bioimaging analysis. Herein, the N-doped carbon dots (N-CDs) with bright orange fluorescence emission at 592 nm were facilely synthesized via p-phenylenediamine as a carbon precursor by microwave method. The as-prepared N-CDs exhibit favorable biocompatibility, excellent water solubility, highly optical stabilities and display sensitive pH response behavior...
November 1, 2018: Talanta
https://www.readbyqxmd.com/read/30062866/small-scale-biological-and-artificial-multidimensional-sensors-for-3d-sensing
#3
REVIEW
Kriti Agarwal, Sehyun Hwang, Aaron Bartnik, Nicholas Buchele, Avishek Mishra, Jeong-Hyun Cho
A vast majority of existing sub-millimeter-scale sensors have a planar, 2D geometry as a result of conventional top-down lithographic procedures. However, 2D sensors often suffer from restricted sensing capability, allowing only partial measurements of 3D quantities. Here, nano/microscale sensors with different geometric (1D, 2D, and 3D) configurations are reviewed to introduce their advantages and limitations when sensing changes in quantities in 3D space. This Review categorizes sensors based on their geometric configuration and sensing capabilities...
July 30, 2018: Small
https://www.readbyqxmd.com/read/30061704/high-throughput-chemical-screening-to-discover-new-modulators-of-microrna-expression-in-living-cells-by-using-graphene-based-biosensor
#4
Soo-Ryoon Ryoo, Yeajee Yim, Young-Kwan Kim, Il-Soo Park, Hee-Kyung Na, Jieon Lee, Hongje Jang, Cheolhee Won, Sungwoo Hong, Sung-Yon Kim, Noo Li Jeon, Joon Myong Song, Dal-Hee Min
MicroRNAs (miRNAs) are important regulatory RNAs that control gene expression in various biological processes. Therefore, control over the disease-related miRNA expression is important both for basic research and for a new class of therapeutic modality to treat serious diseases such as cancer. Here, we present a high-throughput screening strategy to identify small molecules that modulate miRNA expression in living cells. The screen enables simultaneous monitoring of the phenotypic cellular changes associated with the miRNA expression by measuring quantitative fluorescent signals corresponding to target miRNA level in living cells based on a novel biosensor composed of peptide nucleic acid and nano-sized graphene oxide...
July 30, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30053513/highly-selective-and-sensitive-detection-of-staphylococcus-aureus-with-gold-nanoparticle-based-core-shell-nano-biosensor
#5
Reza Shahbazi, Mojtaba Salouti, Bahram Amini, Ahmad Jalilvand, Ebrahim Naderlou, Ali Amini, Arash Shams
Staphylococcus aureus is a gram-positive and opportunistic pathogen that is one of the most common causes of nosocomial infections; therefore, its rapid diagnosis is important and valuable. Today, the use of nanoparticles is expanding due to their unique properties. The purpose of the present study is the determination of S. aureus by a colorimetric method based on gold nanoparticles (AuNPs). Firstly, S. aureus was cultured on both LB media (broth and agar) and their chromosomal DNA was extracted. Afterwards, primers and biosensors were designed based on Protein A sequence data in the gene bank...
July 24, 2018: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/30052647/urea-and-creatinine-detection-on-nano-laminated-gold-thin-film-using-kretschmann-based-surface-plasmon-resonance-biosensor
#6
P Susthitha Menon, Fairus Atida Said, Gan Siew Mei, Dilla Duryha Berhanuddin, Akrajas Ali Umar, Sahbudin Shaari, Burhanuddin Yeop Majlis
This work investigates the surface plasmon resonance (SPR) response of 50-nm thick nano-laminated gold film using Kretschmann-based biosensing for detection of urea and creatinine in solution of various concentrations (non-enzymatic samples). Comparison was made with the presence of urease and creatininase enzymes in the urea and creatinine solutions (enzymatic samples), respectively. Angular interrogation technique was applied using optical wavelengths of 670 nm and 785 nm. The biosensor detects the presence of urea and creatinine at concentrations ranging from 50-800 mM for urea samples and 10-200 mM for creatinine samples...
2018: PloS One
https://www.readbyqxmd.com/read/30041152/nanomaterial-based-biosensors-and-immunosensors-for-quantitative-determination-of-cardiac-troponins
#7
REVIEW
Alireza Nezami, Sadegh Dehghani, Rahim Nosrati, Negar Eskandari, Seyed Mohammad Taghdisi, Gholamreza Karimi
Cardiovascular diseases (CVDs) are the most frequent mortality cause in many countries. The acute myocardial infraction (AMI) is one of the most common types of CVDs. Cardiac troponin I (cTnI) and cardiac troponin T (cTnT) as predominant cardiac infarction biomarkers considered as "gold standard" for diagnosis of acute myocardial infraction (AMI). The restrictions of traditional methods have encouraged the development of highly sensitive and specific methods for cTnI and cTnT detection. The rapid, early, reliable, and cost-effective diagnosis of CVDs not only helps with patient survival, but also save cost and time to prosperous prognosis...
July 18, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30029200/emerging-nano-biosensing-with-suspended-mnp-microbial-extraction-and-eanp-labeling
#8
REVIEW
Leann Lerie Matta, Evangelyn C Alocilja
Emerging nano-biosensing with suspended MNP microbial extraction and EANP labeling may ensure a secure microbe-free food supply, as rapid response detection of microbial contamination is of utmost importance. Many biosensor designs have been proposed over the past two decades, covering a broad range of binding ligands, signal amplification, and detection mechanisms. These designs may consist of self-contained test strips developed from the base up with complicated nanoparticle chemistry and intricate ligand immobilization...
October 15, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29995423/automated-in-vivo-nanosensing-of-breath-borne-protein-biomarkers
#9
Haoxuan Chen, Jing Li, Xiangyu Zhang, Xinyue Li, Maosheng Yao, Gengfeng Zheng
Toxicology and bedside medical condition monitoring is often desired to be both ultrasensitive and noninvasive. However, current biomarker analyses for these purposes are mostly offline and fail to detect low marker quantities. Here, we report a system called dLABer (detection of living animal's exhaled breath biomarker) that integrates living rats, breath sampling, microfluidics, and biosensors for the automated tracking of breath-borne biomarkers. Our data show that dLABer could selectively detect (online) and report differences (of up to 103 -fold) in the levels of inflammation agent interleukin-6 (IL-6) exhaled by rats injected with different ambient particulate matter (PM)...
July 16, 2018: Nano Letters
https://www.readbyqxmd.com/read/29986425/microscale-and-nanoscale-biosensors
#10
EDITORIAL
Beatriz Jurado-Sánchez
The emerge of nanotechnology along with the success of the microelectronics industry has motivated the miniaturization of biosensors into the nano/microscale. This Special Issue highlights recent advances in microscale and nanoscale biosensors, including self-propelled micromotors: their materials, fabrication, and applications. A total of seven papers (five research and two review papers) are included. Different but related topics are covered, from biosensor design (paper strips and digital microfluidic chips) to integrated configurations that monitor metabolites in cellular environments...
July 6, 2018: Biosensors
https://www.readbyqxmd.com/read/29947211/three-dimensional-dna-origami-as-programmable-anchoring-points-for-bioreceptors-in-fiber-optic-surface-plasmon-resonance-biosensing
#11
Devin Daems, Wolfgang Pfeifer, Iene Rutten, Barbara Saccà, Dragana Spasic, Jeroen Lammertyn
Many challenges in biosensing originate from the fact that the all-important nanoarchitecture of the biosensor surface, including precise density and orientation of bioreceptors, is not entirely comprehended. Here, we introduced a three-dimensional DNA origami as a bioreceptor carrier to functionalize the fiber optic surface plasmon resonance (FO-SPR) sensor with nanoscale precision. Starting from a 24-helix bundle, two distinct DNA origami structures were designed to position thrombin-specific aptamers with different densities and distances (27 and 113 nm) from the FO-SPR surface...
July 18, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29941799/nanoscale-biosensors-based-on-self-propelled-objects
#12
REVIEW
Beatriz Jurado-Sánchez
This review provides a comprehensive overview of the latest developments (2016⁻2018 period) in the nano and micromotors field for biosensing applications. Nano and micromotor designs, functionalization, propulsion modes and transduction mechanism are described. A second important part of the review is devoted to novel in vitro and in vivo biosensing schemes. The potential and future prospect of such moving nanoscale biosensors are given in the conclusions.
June 25, 2018: Biosensors
https://www.readbyqxmd.com/read/29933223/nano-biosensing-approaches-on-tuberculosis-defy-of-aptamers
#13
REVIEW
Behrouz Golichenari, Rahim Nosrati, Aref Farokhi-Fard, Khalil Abnous, Farzam Vaziri, Javad Behravan
Tuberculosis is a major global health problem caused by the bacterium Mycobacterium tuberculosis (Mtb) complex. According to WHO reports, 53 million TB patients died from 2000 to 2016. Therefore, early diagnosis of the disease is of great importance for global health care programs. The restrictions of traditional methods have encouraged the development of innovative methods for rapid, reliable, and cost-effective diagnosis of tuberculosis. In recent years, aptamer-based biosensors or aptasensors have drawn great attention to sensitive and accessible detection of tuberculosis...
June 11, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29890392/nano-biosensor-for-highly-sensitive-detection-of-her2-positive-breast-cancer
#14
Razieh Salahandish, Ali Ghaffarinejad, Seyed Morteza Naghib, Keivan Majidzadeh-A, Hossein Zargartalebi, Amir Sanati-Nezhad
Nanocomposite materials have provided a wide range of conductivity, sensitivity, selectivity and linear response for electrochemical biosensors. However, the detection of rare cells at single cell level requires a new class of nanocomposite-coated electrodes with exceptional sensitivity and specificity. We recently developed a construct of gold nanoparticle-grafted functionalized graphene and nanostructured polyaniline (PANI) for high-performance biosensing within a very wide linear response and selective performance...
October 15, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29886189/aptasensor-based-on-fluorophore-quencher-nano-pair-and-smartphone-spectrum-reader-for-on-site-quantification-of-multi-pesticides
#15
Nan Cheng, Yang Song, Qiangqiang Fu, Dan Du, Yunbo Luo, Yong Wang, Wentao Xu, Yuehe Lin
Current techniques for the detection of multi-pesticides are limited by technical complexity, sensitivity and cost. There is an urgent demand of developing new specific recognition elements and sensitive signal readouts for on-site monitoring. In this work, we developed a fluorescent aptamer-based lateral flow biosensor (apta-LFB) integrated with fluorophore-quencher nano-pairs and a smartphone spectrum reader to accomplish triple-target detection of chlorpyrifos, diazinon, and malathion. Aptamers serve as alternative recognition elements instead of antibodies in LFB, offering better specificity and stability...
October 15, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29870901/siderophore-based-biosensors-and-nanosensors-new-approach-on-the-development-of-diagnostic-systems
#16
REVIEW
Rahim Nosrati, Sadegh Dehghani, Bahareh Karimi, Meysam Yousefi, Seyed Mohammad Taghdisi, Khalil Abnous, Mona Alibolandi, Mohammad Ramezani
Siderophores are small organic compounds secreted by microorganisms under iron-depleted conditions which enhance the uptake of iron. Siderophores can play vital roles in ecology, agriculture, bioremediation, biosensor, and medicine. In recent years, the concept of siderophore-based biosensing devices has opened new horizons in high precision detection of various metal ions especially the iron, microorganisms, phosphopeptides, antibiotics as well pesticides. Once combined with nanomaterials, nano-scale siderophore systems provide powerful analytical platforms for detection of low concentration of metal ions and numerous pathogens...
October 15, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29866264/capillary-electrophoresis-analysis-of-affinity-to-assess-carboxylation-of-multi-walled-carbon-nanotubes
#17
Tyler A Davis, Shannon M Patberg, Linda M Sargent, Aleksandr B Stefaniak, Lisa A Holland
Surface oxidation improves the dispersion of carbon nanotubes in aqueous solutions and plays a key role in the development of biosensors, electrochemical detectors and polymer composites. Accurate characterization of the carbon nanotube surface is important because the development of these nano-based applications depends on the degree of functionalization, in particular the amount of carboxylation. Affinity capillary electrophoresis is used to characterize the oxidation of multi-walled carbon nanotubes. A polytryptophan peptide that contains a single arginine residue (WRWWWW) serves as a receptor in affinity capillary electrophoresis to assess the degree of carboxylation...
October 16, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29859844/a-novel-impedimetric-glucose-biosensor-based-on-immobilized-glucose-oxidase-on-a-cuo-chitosan-nanobiocomposite-modified-fto-electrode
#18
Padideh Naderi Asrami, Sayed Ahmad Mozaffari, Mohammad Saber Tehrani, Parviz Aberoomand Azar
This research aims at elaborating on the construction of a novel nanostructured copper oxide (Nano-CuO) sputtered thin film on the conductive fluorinated-tin oxide (FTO) layer that was exploited to immobilize glucose oxidase (GOx) enzyme via chitosan for developing impedimetric glucose biosensing. The distinct Nano-CuO thin film was fabricated by reactive DC magnetron sputtering system at the optimized instrumental deposition conditions. The FTO/Nano-CuO/Chitosan/GOx biosensor containing several layers afforded excellent microenvironment for rapid biocatalytic reaction to glucose...
May 31, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29785801/gold-surface-plasmon-crystal-structure-based-on-polystyrene-template-for-biosensor-application
#19
Min-Zhuo Cheng, Jing Zhang, Dequan Bao, Xiwei Huang
In this communication, we assembled ordered polystyrene (PS) microsphere array as a template with the drop-coating method, and the oxygen plasma was used to etch the template to adjust the spacing between the PS microspheres. Nano-triangular gold array and silver nano-pyramid array were obtained by ion beam sputtering to deposit precious metal gold and silver. We observed the surface morphology of Au and Au/Ag composite films by scanning electron microscope and characterized the films by X-ray diffraction and ultraviolet/visible light spectrophotometer...
May 21, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29783081/recent-technological-advancements-in-tuberculosis-diagnostics-a-review
#20
REVIEW
Shagun Gupta, Vipan Kakkar
Early diagnosis and on-time effective treatment are indispensable for Tuberculosis (TB) control - a life threatening infectious communicable disease. The conventional techniques for diagnosing TB normally take two to three weeks. This delay in diagnosis and further increase in detection complexity due to the emerging risks of XDR-TB (Extensively drug Resistant-TB) and MDR-TB (Multidrug Resistant-TB) are evoking interest of researchers in the field of developing rapid TB detection techniques such as biosensing and other point-of-care (POC) techniques...
September 15, 2018: Biosensors & Bioelectronics
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