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Nano Biosensor

Sang-Hyun Oh, Hatice Altug
Nanoplasmonic structures can tightly confine light onto a material's surface to probe biomolecular interactions not easily accessed by other sensing techniques. New and exciting developments in nanofabrication processes, nano-optical trapping, graphene devices, mid-infrared spectroscopy, and metasurfaces will greatly empower the performance and functionalities of nanoplasmonic sensors.
December 10, 2018: Nature Communications
Stefano Mariani, Valentina Robbiano, Lucanos M Strambini, Aline Debrassi, Gabriela Egri, Lars Dähne, Giuseppe Barillaro
Nanostructured materials premise to revolutionize the label-free biosensing of analytes for clinical applications, leveraging the deeper interaction between materials and analytes with comparable size. However, when the characteristic dimension of the materials reduces to the nanoscale, the surface functionalization for the binding of bioreceptors becomes a complex issue that can affect the performance of label-free biosensors. Here we report on an effective and robust route for surface biofunctionalization of nanostructured materials based on the layer-by-layer (LbL) electrostatic nano-assembly of oppositely-charged polyelectrolytes, which are engineered with bioreceptors to enable label-free detection of target analytes...
December 10, 2018: Nature Communications
Kai U Cormann, Meike Baumgart, Michael Bott
The development of biosensors for in vitro quantification of small molecules such as metabolites or man-made chemicals is still a major challenge. Here we show that engineered variants of the sensory PAS domain of the histidine kinase CitA of the thermophilic bacterium Geobacillus thermoleovorans represent promising alternatives to established biorecognition elements. By combining binding site grafting and rational design we constructed protein variants binding l-malate, ethylmalonate, or the aromatic compound phthalate instead of the native ligand citrate...
December 10, 2018: ACS Synthetic Biology
Amirkhosro Beheshti-Marnani, Abdolhamid Hatefi-Mehrjardi, Zarrin Es'haghi
A simple, low-cost and sensitive label-free aptasensor assembled with assisting reduced graphene oxide nanosheets as the signal amplifier was fabricated and applied for detecting ultra-low levels of Aflatoxin B1(AFB1) through a nano-bio interaction system. The conditions of different modified glassy carbon electrodes as the base of aptasensor were investigated by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The performance of the fabricated aptasensor was evaluated by FESEM, HRTEM and AFM images...
December 1, 2018: Colloids and Surfaces. B, Biointerfaces
Dalibor Breznan, Dharani D Das, Christine MacKinnon-Roy, Stéphane Bernatchez, Abdelhamid Sayari, Myriam Hill, Renaud Vincent, Prem Kumarathasan
Nanoforms of mesoporous silica (mSiNPs) are increasingly applied in medicine, imaging, energy storage, catalysis, biosensors and bioremediation. The impact of their physicochemical properties on health and the environment remain to be elucidated. In this work, newly synthesized mesoporous silica (sizes: 25, 70, 100, 170 and 600 nm; surface functionalization: pristine, C3- and C11-COOH moieties) were assessed for cytotoxicity and induction of inflammatory responses in vitro (A549, THP-1, J774A.1 cells). All toxicity endpoints were integrated to obtain simple descriptors of biological potencies of these mSiNPs...
November 26, 2018: ACS Nano
Min-Sik Kang, Kyu-Hyon Son, Tae-Hoon Lee, Sang-Mok Chang, Sung-Woong Han, Hoon-Kyu Shin
Amyloid β (A β ) is considered to be one of a potential biomarker to monitor Alzheimer's Disease (AD) not only for diagnostic purposes but for early detection. Here we describe a novel nano-biosensor for A β mediated by poly-L-lysine (PLL) which was used for the amplification of detection signal for A β . The indirect enzyme-linked immunosorbent assay (ELISA) method was modified using PLL for the amplification of the A β detection signal. A commercially available ELISA plate was modified by PLL using chemical agent and the amplified amino groups were activated by a chemical agent for the detection of A β ...
March 1, 2019: Journal of Nanoscience and Nanotechnology
Abbas Panahi, Ebrahim Ghafar-Zadeh, Sebastian Magiierowski, Mohammad Hossein Sabour
DNA sequencing is an essential process for determining the nucleotides on a DNA strands and this process is of high importance in medicine and biomedical research. Nanopore based DNA sequencing has been widely researched and analyzed during last years and this is very important to improve all parameters involved in the process of DNA translocating through these nanopours. Ionic current resolution is a key parameter in designing these nanopours for better measuring of this infinitesimal current that is in order of nano A or even pico A...
July 2018: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Ioana Voiculescu, Masaya Toda, Naoki Inomata, Takahito Ono, Fang Li
This review presents several sensors with dimensions at the nano- and micro-scale used for biological applications. Two types of cantilever beams employed as highly sensitive temperature sensors with biological applications will be presented. One type of cantilever beam is fabricated from composite materials and is operated in the deflection mode. In order to achieve the high sensitivity required for detection of heat generated by a single mammalian cell, the cantilever beam temperature sensor presented in this review was microprocessed with a length at the microscale and a thickness in the nanoscale dimension...
August 31, 2018: Micromachines
Sanni Kumar, Suryasnata Tripathy, Anupam Jyoti, Shiv Govind Singh
Sepsis is one of the leading causes of mortality among critically ill patients globally. According to WHO report 2018, it is estimated to affect beyond 30 million people worldwide every year. It causes loss of human lives, which arise from infection and inflammation and long term stay in intensive care unit (ICU) in hospitals. Despite the availability of satisfactory prognostic markers contributing to the diagnosis of sepsis, millions of people die even after admission to the hospitals. Correct and early diagnosis of sepsis leads to rapid administration of appropriate antibiotics can thus potentially avert the attainment to critical stages of sepsis, thereby saving human lives...
January 15, 2019: Biosensors & Bioelectronics
Aruna Chandra Singh, M H Asif, Gautam Bacher, Bengt Danielsson, Magnus Willander, Sunil Bhand
Advances in nanostructured materials have facilitated the development of novel sensitive techniques for detection of environmental and clinical analytes. There is immense need for development of devices that can detect analytes at concentrations as low as few pg mL-1 . The comparable size of nanostructured materials and biomolecules enabled the integration of biological systems with nanometer sized structures. Herein, we demonstrate a Zinc Oxide nanorods (ZnONRs) integrated ultrasensitive label-free biosensor with femtomolar (0...
October 18, 2018: Biosensors & Bioelectronics
Jun Zhu, Jian Lou
In order to improve the low temperature sensitivity of conventional sensors, a plasmonic multifunction temperature sensor with high sensitivity is proposed and investigated systematically in this paper. The sensor consists of two metal layers and two ethanol-sealed elliptical resonators connected to a straight waveguide by two rectangular tubes. We numerically analyzed the transmission characteristics of the Nano-device to assess its performance with the finite element method and achieved great optical properties...
October 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Nader Amini, Mohammad Bagher Gholivand, Mojtaba Shamsipur, Ali Akbar Moosavi Movahedi, Sara Farahi, Mehran Habibi-Rezaei, Afshin Maleki, Reza Rezaee, Kazhal Naderi
Amyloid fibrils were produced in a solution of bovine serum albumin (BSA) in buffer solution in the presence of fructose. The solution was incubated for 20 weeks in the dark. We used glycation induced bovine serum albumin in which fibrilogenesis (nano fibrils) followed by using fluorescence (Thioflavin T), Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) to achieve the size and morphology of fibrils. A novel electrochemical biosensor for the detection of hydrogen peroxide was developed based on immobilizing poly (alizarin yellow R) and amyloid nano-fibrils on glassy carbon electrode (PAYR/AMLNFibs/GCE)...
October 15, 2018: International Journal of Biological Macromolecules
Sunita Kumbhat, Rakhee Gehlot, Kavita Sharma, Urvasini Singh, Vinod Joshi
A surface plasmon resonance (SPR) based immunosensor is presented for highly sensitive and selective detection of 17β-estradiol by the indirect competitive inhibition immuno assay, employing anti-17 β-estradiol antibody as high molecular weight (HMW) interactant. Immobilization of estradiol-BSA conjugate onto the nano thin gold surface was accomplished by covalent amide linkage through self assembled monolayer. The proposed biosensor is simple to fabricate, reproducible and exhibit excellent sensitivity for estrogen (detection limit,1 pg mL-1 ) without any significant interference from structurally similar steroidal hormone, progesterone and non-steroidal compound bisphenol-A...
October 9, 2018: Journal of Pharmaceutical and Biomedical Analysis
Linbo Liu, Ke Chen, Nan Xiang, Zhonghua Ni
Nanomaterials manipulation using dielectrophoresis (DEP) is one of the major research areas that could potentially benefit the micro/nano science for diverse applications, such as microfluidics, nanomachine, and biosensor. The innovation and development of basic theories, methods or applications will have a huge impact on the entire related field. Specifically, for DEP manipulation of nanomaterials, improvements in comprehensive performance of accuracy, flexibility and scale could promote broader applications in micro/nano science...
October 5, 2018: Electrophoresis
Marianna Pannico, Anna Calarco, Gianfranco Peluso, Pellegrino Musto
In the present contribution the fabrication and characterization of functionalized gold nanospheres of uniform shape and controlled size is reported. These nano-objects are intended to be used as Surface Enhanced Raman Spectroscopy (SERS) sensors for in-vitro cellular uptake and localization. Thiophenol was used as molecular reporter and was bound to the Au surface by a chemisorption process in aqueous solution. The obtained colloidal solution was highly stable and no aggregation of the single nanospheres into larger clusters was observed...
October 4, 2018: Biosensors
Yibo Zhu, Zhaoyi Li, Zhuang Hao, Christopher DiMarco, Panita Maturavongsadit, Yufeng Hao, Ming Lu, Aaron Stein, Qian Wang, James Hone, Nanfang Yu, Qiao Lin
Optical devices are highly attractive for biosensing as they can not only enable quantitative measurements of analytes but also provide information on molecular structures. Unfortunately, typical refractive index-based optical sensors do not have sufficient sensitivity to probe the binding of low-molecular-weight analytes. Non-optical devices such as field-effect transistors can be more sensitive but do not offer some of the significant features of optical devices, particularly molecular fingerprinting. We present optical conductivity-based mid-infrared (mid-IR) biosensors that allow for sensitive and quantitative measurements of low-molecular-weight analytes as well as the enhancement of spectral fingerprints...
2018: Light, Science & Applications
Muhammad Adeel, Muhammad Bilal, Tahir Rasheed, Ashutosh Sharma, Hafiz M N Iqbal
Graphene-based nanomaterials have gained high research interest in different fields related to proteins and thus are rapidly becoming the most widely investigated carbon-based materials. Their exceptional physiochemical properties such as electrical, optical, thermal and mechanical strength enable graphene to render graphene-based nanostructured materials suitable for applications in different fields such as electroanalytical chemistry, electrochemical sensors and immobilization of biomolecules and enzymes...
December 2018: International Journal of Biological Macromolecules
Seung-Woo Lee, Tae-Hyung Kang, Sung Ku Lee, Ki-Young Lee, Hyunjung Yi
Realizing high-performance electrochemical biosensors in a simple contact-printing-based approach significantly increases the applicability of integrated flexible biosensors. Herein, an enzyme-sticker-based approach that enables flexible and multielectrochemical sensors via simple contact-transfer printing is reported. The enzyme sticker consists of an enzymatic conductive network film and a polymeric support. The enzyme-incorporated nanostructured conductive network showing an efficient electrical coupling was assembled via the hydrodynamic layer-by-layer assembly of redox enzymes, polyelectrolytes, single-walled carbon nanotubes, and a biological glue material, M13 phage...
October 24, 2018: ACS Applied Materials & Interfaces
Hendrikje R Neumann, Christine Selhuber-Unkel
The production of micrometer-sized structures comprised of nanoparticles in defined patterns and densities is highly important in many fields, ranging from nano-optics to biosensor technologies and biomaterials. A well-established method to fabricate quasi-hexagonal patterns of metal nanoparticles is block copolymer micelle nanolithography, which relies on the self-assembly of metal-loaded micelles on surfaces by a dip-coating or spin-coating process. Using this method, the spacing of the nanoparticles is controlled by the size of the micelles and by the coating conditions...
2018: Beilstein Journal of Nanotechnology
Farhana Sharmin Diba, Andrew Boden, Helmut Thissen, Mrinal Bhave, Peter Kingshott, Peng-Yuan Wang
The organization of matter into hierarchical structures is a fundamental characteristic of functional materials and living organisms. Binary colloidal crystal (BCC) systems present a diversified range of nanotopographic structures where large and small colloidal particles simultaneously self-assemble into either 2D monolayer or 3D hierarchical crystal lattices. More importantly, understanding how BCCs form opens up the possibility to fabricate more complex systems such as ternary or quaternary colloidal crystals...
November 2018: Advances in Colloid and Interface Science
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