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ovarian granulosa cell

Guo-Liang Zhang, Jun-Lin Song, Chuan-Liang Ji, Yu-Long Feng, Jie Yu, Charles M Nyachoti, Gong-She Yang
Zearalenone (ZEA) is a natural contaminant existing in food and feed products that exhibits a negative effect on domestic animals' reproduction. Donkeys possess high economic value in China and are at risk of exposure to ZEA. However, few information is available on ZEA-induced toxicity and no report on toxicity in donkeys can be found in scientific literature. We investigated the biological effects of ZEA exposure on donkey granulosa cells (dGCs) by using RNA-seq analysis. ZEA at 10 and 30 μM were administered to GCs within 72 h of in vitro culture...
2018: Frontiers in Genetics
Maria Éllida S Bezerra, Bruna B Gouveia, Ricássio S Barberino, Vanúzia G Menezes, Taís J S Macedo, Agnes Y P Cavalcante, Alane P O Monte, Jamile M S Santos, Maria Helena T Matos
We aimed to study the effects of resveratrol on the morphology, DNA fragmentation, follicular activation and cell proliferation after in vitro culture of ovine ovarian tissue, and to verify if PI3K pathway is involved in resveratrol action in the sheep ovary. Ovaries were collected and divided into fragments. One fragment was fixed for histology (fresh control). The remaining fragments were cultured for 7 days in control medium (α-MEM+ ) alone or with resveratrol (2, 10 or 30 µM). After culture, ovarian tissue was destined to morphological analysis...
August 13, 2018: Reproduction in Domestic Animals, Zuchthygiene
Islam M Saadeldin, Ayman Abdel-Aziz Swelum, Mona Elsafadi, Amer Mahmood, Musaad Alfayez, Abdullah N Alowaimer
The mammalian ovary is a highly dynamic organ, in which proliferation and differentiation occur constantly during the entire life span, particularly in camels that are characterized by a follicular wave pattern and induced ovulation. Granulosa cells are the main cells of mature follicles. Two distinct cell types, namely, the mural and cumulus granulosa cells are distinguished on the basis of antral fluid increase. The multipotency of follicular fluid and the luteinizing cell were recently demonstrated. However, reports regarding the plasticity of cumulus cells are lacking...
September 15, 2018: Theriogenology
Ai Ai, Ying Xiong, Beiling Wu, Jiajia Lin, Yongyi Huang, Yilin Cao, Te Liu
Tripterygium glycosides (TGs) are chemotherapeutic drugs and immunosuppressant agents for the treatment of cancer and autoimmune diseases. We have previously reported that TGs induces premature ovarian failure (POF) by inducing cytotoxicity in ovarian granulosa cells (OGCs). Hence, we report that TGs suppress the expression of the Hippo-YAP/TAZ pathway in murine OGCs in vitro and in vivo. We found that the expressions of miR-181b, miR-15a, and miR-30d, were elevated significantly in the POF. Luciferase reporter assays confirmed that miR-15a targets Lats1 through a miR-15a binding site in the Lats1 3'UTR...
August 6, 2018: Gene
Ketan Shrestha, Rina Meidan
During the periovulatory period the profile of FGF2 coincides with elevated PGE2 levels. We investigated therefore whether PGE2 can directly stimulate FGF2 production in bovine granulosa cells and if so, which PTGER type and signaling cascades are involved. We found that PGE2 temporally stimulated FGF2. Accordingly, endoperoxide-synthase2-silenced cells, exhibiting low endogenous PGE2 levels, had reduced FGF2. Furthermore, elevation of viable granulosa cell numbers by PGE2 was abolished with FGF2 receptor1 inhibitor, suggesting that FGF2 mediates this action of PGE2...
July 31, 2018: Endocrinology
Camila P Almeida, Márcia C F Ferreira, Camila O da Silveira, Jacira R Campos, Isabella T Borges, Pedro G Baeta, Felipe H S Silva, Fernando M Reis, Helen L Del Puerto
The quality of oocytes depends on interactions with surrounding granulosa cells. Granulosa cells are essential in normal follicular maturation process since they produce steroidal hormones and growth factors, and they play a crucial role in follicular atresia. The success in reproductive biology and medicine depends on reliable assessment of oocyte and embryo viability which presently mainly bases on oocyte and embryo morphology. Recent investigations have tried to establish an evaluation system for oocyte quality and to predict outcome of in vitro fertilization (IVF) based on the incidence of granulosa cells and cumulus cells apoptosis...
August 6, 2018: Cell Biology International
Kyosuke Kagami, Yohei Shinmyo, Masanori Ono, Hiroshi Kawasaki, Hiroshi Fujiwara
BACKGROUND: Recently, we demonstrated the three-dimensional (3D) localization of murine trophoblast giant cells in the pregnant uterus using a modified Clear Unobstructed Brain Imaging Cocktails and Computational analysis (CUBIC) tissue-clearing method and hybrid construct consisting of the cytomegalovirus enhancer fused to the chicken beta-actin promoter (CAG) conjugated enhanced green fluorescent protein (EGFP) transgenic mice. In this study, we applied this method to obtain a transparent whole-image of the ovary and observed the 3D localization of individual oocytes in the developing follicles...
August 2, 2018: Reproductive Biology and Endocrinology: RB&E
Fernanda R C L Almeida, Natasja G J Costermans, Nicoline M Soede, Annelies Bunschoten, Jaap Keijer, Bas Kemp, Katja J Teerds
BACKGROUND: Anti-Müllerian hormone (AMH) is expressed by granulosa cells of developing follicles and plays an inhibiting role in the cyclic process of follicular recruitment by determining follicle-stimulating hormone threshold levels. Knowledge of AMH expression in the porcine ovary is important to understand the reproductive efficiency in female pigs. RESEARCH AIM: In the present study we investigated the expression of AMH during follicular development in prepubertal and adult female pigs by immunohistochemistry, laser capture micro-dissection and RT-qPCR...
2018: PloS One
Sambit Roy, Divya Gandra, Christina Seger, Anindita Biswas, Vitaly A Kushnir, Norbert Gleicher, T Rajendra Kumar, Aritro Sen
Anti-Müllerian hormone (AMH) produced by ovarian granulosa cells (GCs) plays a crucial role in ovarian function. It is used as a diagnostic and/or prognostic marker of fertility as well as for pathophysiological conditions in women. In this study, we investigated the underlying mechanism for regulation of AMH expression in GCs using primary mouse GCs and a human GC tumor -derived KGN cell line. We find that growth differentiation factor 9 (GDF9) and bone morphogenetic factor 15 (BMP15) together (GDF9+BMP15), but not when tested separately, significantly induce AMH expression, in vitro and in vivo (serum AMH)...
July 27, 2018: Endocrinology
(no author information available yet)
[This corrects the article DOI: 10.4103/0366-6999.235864].
August 5, 2018: Chinese Medical Journal
G M Ishak, S T Bashir, G A Dutra, G D A Gastal, M O Gastal, C A Cavinder, J M Feugang, E L Gastal
BACKGROUND: In vivo studies involving molecular markers of the follicle wall associated with follicular fluid (FF) milieu are crucial for a better understanding of follicle dynamics. The inability to obtain in vivo samples of antral follicle wall (granulosa and theca cells) without jeopardizing ovarian function has restricted advancement in knowledge of folliculogenesis in several species. The purpose of this study in mares was to develop and validate a novel, minimally invasive in vivo technique for simultaneous collection of follicle wall biopsy (FWB) and FF samples, and repeated collection from the same individual, during different stages of antral follicle development...
July 28, 2018: Reproductive Biology and Endocrinology: RB&E
Warakorn Cheewasopit, Mhairi Laird, Claire Glister, Philip G Knight
Myostatin plays a negative role in skeletal muscle growth regulation but its potential role in the ovary has received little attention. Here, we first examined relative expression of myostatin (MSTN), myostatin receptors (ACVR1B, ACVR2B and TGFBR1) and binding protein, follistatin (FST), in granulosa (GC) and theca (TC) cells of developing bovine follicles. Secondly, using primary GC and TC cultures, we investigated whether myostatin affects steroidogenesis and cell number. Thirdly, effects of gonadotropins and other factors on MSTN expression in GC and TC were examined...
July 27, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
Zeeshan Ahmad Khan, Rajendra Kumar Labala, Thangal Yumnamcha, Sijagurumayum Dharmajyoti Devi, Gopinath Mondal, Haobijam Sanjita Devi, Chongtham Rajiv, Rupjyoti Bharali, Asamanja Chattoraj
The ALAN is drawing the attention of researchers and environmentalists for its ever-increasing evidence on its capacity of "desynchronization" of organismal physiology. Photoperiod and circadian cycles are critical parameters to influence the biology of reproduction in several animals, including fish. The present study is the first proof of the development of an ovarian tumour with the effect of light in zebrafish (Danio rerio), an excellent model for circadian-related studies. Results of three experimental conditions, continuous light for one week, LLW, one month, LLM, and for one year, LLY revealed a clear desynchronization of clock associated genes (Clock1a, Bmal1a, Per2, and Cry2a)...
July 1, 2018: Science of the Total Environment
Sarah Dallel, Igor Tauveron, Florence Brugnon, Silvère Baron, Jean Marc A Lobaccaro, Salwan Maqdasy
A close relationship exists between cholesterol and female reproductive physiology. Indeed, cholesterol is crucial for steroid synthesis by ovary and placenta, and primordial for cell structure during folliculogenesis. Furthermore, oxysterols, cholesterol-derived ligands, play a potential role in oocyte maturation. Anomalies of cholesterol metabolism are frequently linked to infertility. However, little is known about the molecular mechanisms. In parallel, increasing evidence describing the biological roles of liver X receptors (LXRs) in the regulation of steroid synthesis and inflammation, two processes necessary for follicle maturation and ovulation...
July 25, 2018: International Journal of Molecular Sciences
Alexandra Sanfins, Patrícia Rodrigues, David F Albertini
Understanding the physiology underlying the complex dialog between the oocyte and its surrounding somatic cells within the ovarian follicle has been crucial in defining optimal procedures for the development of clinical approaches in ART for women suffering from infertility and ovarian dysfunction. Recent studies have implicated oocyte-secreted factors like growth differentiation factor 9 (GDF-9) and bone morphogenetic protein 15 (BMP-15), members of the transforming growth factor-beta (TGFβ) superfamily, as potent regulators of folliculogenesis and ovulation...
July 23, 2018: Journal of Assisted Reproduction and Genetics
Saara Bryk, Eero Pukkala, Anniina Färkkilä, Markku Heikinheimo, Leila Unkila-Kallio, Annika Riska
OBJECTIVE: The aim of this study was to determine the incidence of new primary malignancies after adult-type granulosa cell tumor (AGCT) and the incidence of AGCT after breast and uterine cancer using nationwide population-based registry data. METHODS: We used the Finnish Cancer Registry to identify all patients diagnosed with AGCT in 1968 to 2013 (n = 986). The number of subsequent primary malignancies among women with AGCT and the number of AGCTs in women with previous breast or uterine cancer were compared with the expected number of cases and expressed as standardized incidence ratios (SIRs)...
July 20, 2018: International Journal of Gynecological Cancer
J Grosbois, I Demeestere
STUDY QUESTION: How does biochemical stimulation or inhibition of the PI3K/Akt/mTOR pathway affect the activation and the growth of human primordial follicles in vitro? SUMMARY ANSWER: The PI3K/Akt activators act synergistically with the Hippo signaling pathway, disrupted by tissue fragmentation, to accelerate primordial follicles recruitment while mTORC1 inhibitor partially prevents the massive spontaneous activation. WHAT IS KNOWN ALREADY: Hippo disruption caused by ovarian fragmentation and exposure to PI3K/Akt activators have been successfully used to activate resting follicles prior to grafting in patients with premature ovarian insufficiency...
July 19, 2018: Human Reproduction
Xiafei Fu, Yuanli He, Xuefeng Wang, Dongxian Peng, Xiaoying Chen, Xinran Li, Qing Wan
BACKGROUND/AIMS: Several miRNAs have been reported to be involved in the pathogenesis of polycystic ovarian syndrome (PCOS). However, the biological roles of miR-16 and its molecular mechanisms in PCOS development remain to be elucidated. METHODS: qRT-PCR was performed to detect the expression levels of miR-16 and programmed cell death protein 4 (PDCD4). GCs proliferation, cell cycle distribution and apoptosis were examined by MTT assay and flow cytometry analysis...
July 19, 2018: Cellular Physiology and Biochemistry
Alexander V Makarevich, Martina Földešiová, Juraj Pivko, Elena Kubovičová, Peter Chrenek
Follicle atresia in mammals is a universal phenomenon characteristic by degenerative morphological changes in granulosa and theca cells. The unfavourable effect of milk production in relation to fertility has been studied starting from the 70s of the last century; however, there is no unambiguous and persuasive data on association of ovarian atresia with milk yield of dairy cows. The aim of this study was to define histological signs of ovarian follicle atresia in dairy cows in relation to their milk production...
July 18, 2018: Anatomia, Histologia, Embryologia
Pengju Zhang, Jun Wang, Hongyan Lang, Weixia Wang, Xiaohui Liu, Haiyan Liu, Chengcheng Tan, Xintao Li, Yumin Zhao, Xinghong Wu
Cyclic AMP response element-binding protein 1 (CREB1), a member of the CREB family, is known to be involved in follicular growth, ovulation, and ovarian disease. However, the physiological function of CREB1 in mouse granulosa cells (mGCs) remains lagerly unknown. The aim of this study was to determine the role of CREB1 in mGCs by knocking down CREB1 expression. CREB1 knock-down in mGCs at the mRNA and protein levels, was confirmed by quantitative real-time polymerase chain reaction and western blot. Results of enzyme linked immunosorbent assay revealed that CREB1 knockdown significantly decreased the concentrations of estradiol (E2) and progesterone (P4) in mGCs...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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