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microvascular density

Valentin Demidov, Xiao Zhao, Olga Demidova, Hilary Y M Pang, Costel Flueraru, Fei-Fei Liu, I Alex Vitkin
Radiation therapy (RT) is widely and effectively used for cancer treatment but can also cause deleterious side effects, such as a late-toxicity complication called radiation-induced fibrosis (RIF). Accurate diagnosis of RIF requires analysis of histological sections to assess extracellular matrix infiltration. This is invasive, prone to sampling limitations, and thus rarely used; instead, current practice relies on subjective clinical surrogates, including visual observation, palpation, and patient symptomatology questionnaires...
October 2018: Journal of Biomedical Optics
Anna Adamska, Aleksandra Araszkiewicz, Stanislaw Pilacinski, Agnieszka Gandecka, Agata Grzelka, Katarzyna Kowalska, Agnieszka Malinska, Michal Nowicki, Dorota Zozulinska-Ziolkiewicz
In patients with diabetes, functional changes in microcirculation and subclinical vascular pathology precede clinical manifestation of microangiopathic complications. The objective of this study was to evaluate the association between established vascular risk factors and density, maturity, and reactivity of dermal blood vessels in adults with type 1 diabetes (DM1). We included 148 DM1 patients (87 men) with a median (IQR) age of 40.5 (30.5-49) years and a median diabetes duration of 21 (17-29.5) years. The control group consisted of 13 healthy volunteers (6 men) with a median (IQR) age of 36 (31-43)...
October 9, 2018: Microvascular Research
Julia Hafner, Sonja Karst, Stefan Sacu, Christoph Scholda, Eleonore Pablik, Ursula Schmidt-Erfurth
PURPOSE: The pathophysiology of diabetic neurodegeneration and microvasculopathy remains controversial. Neurosensory layer thickness and corneal nerve fibre loss represent potential biomarkers of neuropathy. The purpose of this cross-sectional study was to determine the correlation between these neurodegenerative features and their association with retinal microvascular integrity in patients with type II diabetes without retinopathy. METHODS: Nerve fibre length (NFL), density (NFD) and branch density (NBD) were assessed using corneal confocal microscopy...
October 11, 2018: Acta Ophthalmologica
Gilda Cennamo, Vittoria Lanni, Rosa Abbate, Nunzio Velotti, Federica Fossataro, Federica Sparnelli, Mario R Romano, Giuseppe de Crecchio, Giovanni Cennamo
AIM: Macular pigment density and microvascular density on optical coherence tomography angiography (OCTA) were measured in a cohort of type 1 diabetes mellitus (T1DM) patients with retinopathy in the attempt to shed light on the pathophysiology of this condition. METHODS: Eighty-two consecutive eyes of 59 patients with diabetic retinopathy examined at the Eye Clinic of the University of Naples Federico II from November 2016 to April 2017 were enrolled in this prospective study...
October 11, 2018: Ophthalmic Research
Qinqin Zhang, Kasra A Rezaei, Steven S Saraf, Zhongdi Chu, Fupeng Wang, Ruikang K Wang
Background: To implement an ultra-wide optical coherence tomography angiography imaging (UW-OCTA) modality in eyes with diabetic retinopathy (DR) with the aim of quantifying the burden of microvascular disease at baseline and subsequent clinic visits. Methods: UW-OCTA was implemented on a 1,060 nm swept source (SS) OCTA engine running at 100 kHz A-line rate with a motion tracking mechanism. A montage scanning protocol was used to capture a 100-degree field of view (FOV) using a 4×4 grid of sixteen total individual 6×6 mm2 scans...
September 2018: Quantitative Imaging in Medicine and Surgery
A A Oraevsky, B Clingman, J Zalev, A T Stavros, W T Yang, J R Parikh
Optoacoustic imaging, based on the differences in optical contrast of blood hemoglobin and oxyhemoglobin, is uniquely suited for the detection of breast vasculature and tumor microvasculature with the inherent capability to differentiate hypoxic from the normally oxygenated tissue. We describe technological details of the clinical ultrasound (US) system with optoacoustic (OA) imaging capabilities developed specifically for diagnostic imaging of breast cancer. The combined OA/US system provides co-registered and fused images of breast morphology based upon gray scale US with the functional parameters of total hemoglobin and blood oxygen saturation in the tumor angiogenesis related microvasculature based upon OA images...
December 2018: Photoacoustics
Zhenmin Jiang, Xiucun Li, Miao Chen, Laijin Lu, Xu Gong
BACKGROUND: Experimental evidence suggests that endogenous vascular endothelial growth factor (VEGF) may play a major role in the surgical delay phenomenon. The purpose of this study was to investigate the effect of the endogenous vascular endothelial growth factor on flap surgical delay. METHODS: A total of 82 adult male Sprague-Dawley rats with an average weight of 330g were used for these experiments. These experiments were then conducted in two parts. In part 1, thirty-two rats were used to assess the effectiveness of VEGF inhibitor through western blot assay and ELISA...
October 9, 2018: Plastic and Reconstructive Surgery
Yoichi Takakusagi, Sarwat Naz, Kaori Takakusagi, Masahiro Ishima, Hiroshi Murata, Keisuke Ohta, Masahiko Miura, Fumio Sugawara, Kengo Sakaguchi, Shun Kishimoto, Jeeva P Munasinghe, James B Mitchell, Murali C Krishna
Hypoxic zones in solid tumors contribute to radioresistance, and pharmacological agents that increase tumor oxygenation prior to radiation, including anti-angiogenic drugs, can enhance treatment response to radiotherapy. Although such strategies have been applied, imaging assessments of tumor oxygenation to identify an optimum time window for radiotherapy have not been fully explored. In this study, we investigated the effects of alpha-sulfoquinovosylacyl-1,3-propanediol (SQAP; a synthetic derivative of an anti-angiogenic agent) on the tumor microenvironment in terms of oxygen partial pressure (pO2), oxyhemoglobin saturation (sO2), blood perfusion, and microvessel density using electron paramagnetic resonance imaging, photoacoustic imaging, dynamic contrast-enhanced MRI with Gd-DTPA injection, and T2*-weighted imaging with ultrasmall superparamagnetic iron oxide (USPIO) contrast...
October 9, 2018: Cancer Research
Muzaffer Akkoca, Suzan Emel Usanmaz, Serhat Tokgöz, Cüneyt Köksoy, Emine Demirel Yilmaz
OBJECTIVE: The aim of this long-term follow-up study was to investigate the association of local and systemic cardiovascular complications with endothelium-dependent and-independent microvascular relaxations and blood biomarkers and biochemicals in patients with peripheral arterial disease (PAD) caused by atherosclerosis. METHODS: This prospective study included 67 patients with PAD who had not undergone any endovascular intervention, peripheral arterial surgery, or major amputation...
October 2018: Anatolian Journal of Cardiology
Barbara Ruaro, Andrea Casabella, Sabrina Paolino, Carmen Pizzorni, Massimo Ghio, Chiara Seriolo, Luigi Molfetta, Patrizio Odetti, Vanessa Smith, Maurizio Cutolo
The aim of this research was to determine any correlations between Dickkopf-1 serum levels (Dkk-1, a natural inhibitor of the Wnt signaling pathway promoting osteoclastogenesis) and the Trabecular Bone Score (TBS), in systemic sclerosis (SSc) and rheumatoid arthritis (RA) patients. It also aimed at determining any difference in Dkk-1 serum levels between SSc and RA patients and a control group (CNT) of healthy subjects. A prospective study was carried out in 60 SSc and 60 RA patients and 60 CNT. Dkk-1 serum levels were evaluated by the ELISA method (Quantikine Human Dkk-1 Immunoassay, R&D System, Minneapolis, USA)...
October 6, 2018: Clinical Rheumatology
Shameena Bake, Andre Okoreeh, Homa Khosravian, Farida Sohrabji
Our previous studies showed that Insulin-like Growth Factor (IGF)-1 reduced blood brain barrier pemeability and decreased infarct volume caused by middle cerebral artery occlusion (MCAo) in middle aged female rats. Similarly, cultures of primary brain microvessel endothelial cells from middle-aged female rats and exposed to stroke-like conditions (oxygen glucose deprivation; OGD) confirmed that IGF-1 reduced dye transfer across this cell monolayer. Surprisingly, IGF-1 did not attenuate endothelial cell death caused by OGD...
October 1, 2018: Experimental Neurology
Philipp M Kazmierczak, Neal C Burton, Georg Keinrath, Heidrun Hirner-Eppeneder, Moritz J Schneider, Ralf S Eschbach, Maurice Heimer, Olga Solyanik, Andrei Todica, Maximilian F Reiser, Jens Ricke, Clemens C Cyran
PURPOSE: To investigate αvβ3-integrin-targeted optoacoustic imaging and MRI for monitoring a BRAF/MEK inhibitor combination therapy in a murine model of human melanoma. MATERIALS AND METHODS: Human BRAF V600E-positive melanoma xenograft (A375)-bearing Balb/c nude mice (n = 10) were imaged before (day 0) and after (day 7) a BRAF/MEK inhibitor combination therapy (encorafenib, 1.3 mg/kg/d; binimetinib, 0.6 mg/kg/d, n = 5) or placebo (n = 5), respectively. Optoacoustic imaging was performed on a preclinical system unenhanced and 5 h after i...
2018: PloS One
Hong Guo, Houyi Kang, Haipeng Tong, Xuesong Du, Heng Liu, Yong Tan, Yizeng Yang, Sumei Wang, Weiguo Zhang
OBJECTIVES: Vessel size imaging (VSI) could reveal average microvessel diameter. The aim was to investigate microvascular characteristics and the efficacy of VSI in lower-grade glioma (LGG) grading and subtype differentiation based on 2016 classification of central nervous system tumours. METHODS: Fifty-seven LGG (grade II/III, 36/21) patients who received VSI examination before surgery were retrospectively analysed. The average (Rmean ) and maximum (Rmax ) vessel size indexes were obtained...
October 1, 2018: European Radiology
Emily Burrage, Kent L Marshall, Nalini Santanam, Paul D Chantler
Maintenance of adequate tissue perfusion through a dense network of cerebral microvessels is critical for the perseveration of normal brain function. Regulation of the cerebral blood flow has to ensure adequate delivery of nutrients and oxygen with moment-to-moment adjustments to avoid both hypo- and hyper-perfusion of the brain tissue. Even mild impairments of cerebral blood flow regulation can have significant implications on brain function. Evidence suggests that chronic stress and depression elicits multifaceted functional impairments to the cerebral microcirculation, which plays a critical role in brain health and the pathogenesis of stress-related cognitive impairment and cerebrovascular events...
April 2018: Brain Circulation
Marie Billaud, Jennifer C Hill, Tara D Richards, Thomas G Gleason, Julie A Phillippi
Human ascending aortic aneurysms characteristically exhibit cystic medial degeneration of the aortic wall encompassing elastin degeneration, proteoglycan accumulation and smooth muscle cell loss. Most studies have focused on the aortic media and there is a limited understanding of the importance of the adventitial layer in the setting of human aneurysmal disease. We recently demonstrated that the adventitial ECM contains key angiogenic factors that are downregulated in aneurysmal aortic specimens. In this study, we investigated the adventitial microvascular network (vasa vasorum) of aneurysmal aortic specimens of different etiology and hypothesized that the vasa vasorum is disrupted in patients with ascending aortic aneurysm...
2018: Frontiers in Cardiovascular Medicine
Chang Jiang, Yu-Hua Huang, Jia-Bin Lu, Yuan-Zhong Yang, Hui-Lan Rao, Bei Zhang, Wen-Zhuo He, Liang-Ping Xia
Purpose: Tumor vessels supported by perivascular cells have been implicated in the failure of some anti-angiogenic agents. The relationship between perivascular cell coverage (PC) and bevacizumab efficacy in metastatic colorectal cancer (mCRC) was analyzed. Patients and methods: A total of 284 consecutive mCRC patients who received first-line chemotherapy with or without bevacizumab from 2007-2014 in Sun Yat-Sen University Cancer Center were analyzed. Immunohistochemical double-stain for the perivascular cell marker alpha-smooth muscle actin and endothelial cell (cluster of differentiation 31) was performed to characterize the intratumoral microvascular density...
2018: Cancer Management and Research
Xiyang Sun, Ying Chen, Hui Zhao, Guanglei Qiao, Meiyang Liu, Chunlei Zhang, Daxiang Cui, Lijun Ma
Development of safe, efficient nanocomplex for targeted imaging and therapy of cancer stem cells in brain glioma has become a great challenge. Herein, a low-density lipoprotein receptor-related protein and a RNA aptamer bound CD133 were used as dual-targeting ligands to prepare dual-modified cationic liposomes (DP-CLPs) loaded with survivin siRNA and paclitaxel (DP-CLPs-PTX-siRNA) for actively targeting imaging and treating CD133+ glioma stem cells after passing through the blood-brain barrier. After being administrated with DP-CLPs-PTX-siRNA nanocomplex, DP-CLPs showed a persistent target ability to bind glioma cells and brain microvascular endothelial cells (BCECs) and to deliver drugs (PTX/siRNA) to CD133+ glioma stem cells...
November 2018: Drug Delivery
Jeremy A Antonyshyn, Meghan J McFadden, Anthony O Gramolini, Stefan O P Hofer, J Paul Santerre
Considerable effort has been directed toward deriving endothelial cells (ECs) from adipose-derived mesenchymal stem cells (ASCs) since 2004, when it was first suggested that ECs and adipocytes share a common progenitor. While the capacity of ASCs to express endothelial markers has been repeatedly demonstrated, none constitute conclusive evidence of an endothelial phenotype as all reported markers have been detected in other, non-endothelial cell types. In this study, quantitative phenotypic comparisons to representative EC controls were used to determine the extent of endothelial differentiation being achieved with ASCs...
September 30, 2018: Stem Cells Translational Medicine
Hironori Uehara, Timilai Lesuma, Parker Stocking, Nathan Jensen, Sangeetha Ravi Kumar, Mingyang Aaron Zhang, Susie Choi, Xiaohui Zhang, Bonnie Archer, Lara Carroll, Balamurali K Ambati
Optical coherence tomography (OCT) angiography is a dye-free and non-invasive angiography which allows visualization of retinal and choroid vascular flow, enabling observation of highly permeable and three dimensional vasculature. Although OCT angiography is providing new insights in human retinal and choroidal diseases, a few studies have been reported in experimental mice. In this study, to determine the potential of OCT angiography in experimental mice, we sought to examine whether OCT angiography can detect vascular change in type I diabetic mice...
September 27, 2018: Experimental Eye Research
Gabriella O Lopes, Paulo Farinatti, Karynne Grutter, Daniel J Medeiros-Lima, Cristiane Matsuura, Ricardo B Oliveira, Eliete Bouskela, Daniel A Bottino, Fabiana Mucillo, Eduardo Tibiriçá, Juliana P Borges
This study compared macro- and microvascular endothelial function and redox status in active vs. inactive HIV-infected patients (HIVP) under antiretroviral therapy. Using a cross-sectional design, macro- and microvascular reactivity, systemic microvascular density, and oxidative stress were compared between 19 HIVP (53.1±6.1 yr) enrolled in a multimodal training program (aerobic, strength and flexibility exercises) for at least 12 months (60-min sessions performed 3 times/week with moderate intensity) vs. 25 sedentary HIVP (51...
September 29, 2018: Scandinavian Journal of Medicine & Science in Sports
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