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Manel Vera, Sergi Torramade-Moix, Susana Martin-Rodriguez, Aleix Cases, Josep M Cruzado, Jose Rivera, Gines Escolar, Marta Palomo, Maribel Diaz-Ricart
BACKGROUND/AIMS: Accelerated atherosclerosis in chronic kidney disease (CKD) is preceded by endothelial dysfunction (ED), which exhibits a proinflammatory and prothrombotic phenotype and enhanced oxidative stress. In this study, the effect of several compounds with anti-inflammatory and/or antioxidant properties on uremia-induced endothelial dysfunction has been evaluated in an in vitro model. METHODS: Endothelial cells (ECs) were exposed to sera from uremic patients in the absence and presence of the flavonoids apigenin, genistein and quercetin, the antioxidant enzyme mimetics (AEM) ebselen (glutathione peroxidase mimetic), EUK-134 and EUK-118 (both superoxide dismutase mimetics), and the pharmacological drug N-acetylcysteine (NAC)...
November 27, 2018: Cellular Physiology and Biochemistry
Roser Guiteras, Anna Sola, Maria Flaquer, Anna Manonelles, Georgina Hotter, Josep M Cruzado
Alternatively activated macrophages (M2) have regenerative properties and shown promise as cell therapy in chronic kidney disease. However, M2 plasticity is one of the major hurdles to overcome. Our previous studies showed that genetically modified macrophages stabilized by neutrophil gelatinase-associated lipocalin (NGAL) were able to preserve their M2 phenotype. Nowadays, little is known about M2 macrophage effects in diabetic kidney disease (DKD). The aim of the study was to investigate the therapeutic effect of both bone marrow-derived M2 (BM-фM2) and ф-NGAL macrophages in the db/db mice...
November 8, 2018: Journal of Cellular and Molecular Medicine
Alba Santos García, Nicolás Macías Carmona, Almudena Vega Martínez, Soraya Abad Estébanez, Tania Linares Grávalos, Inés Aragoncillo Sauco, Ursula Verdalles Guzmán, Nayara Panizo González, Leonidas Cruzado Vega, Juan Manuel López Gómez
INTRODUCTION: The aim of this study is to compare molecule removal and albumin leakage in postdilution online hemodiafiltration with different high-flux dialyzers. METHODS: We studied seven high-flux dialyzers (Polyflux 210H®, Evodial 2.2®, FxCordiax1000®, Elisio21H®, TS-2.1SL®, XevontaHi20®, VitaPES 210-HF®) in 6 patients. The reduction ratio (RR) of small- and middle-sized molecules was calculated. Dialysate samples were collected to estimate the albumin leakage...
October 27, 2018: Hemodialysis International
Anna Manonelles, Roser Guiteras, Edoardo Melilli, Elena Lazzeri, Montse Goma, Elena Crespo, Oriol Bestard, Anna Sola, Paola Romagnani, Josep M Cruzado
Long-term kidney transplant outcomes have reached mild improvements recently. Parietal epithelial cells (PECs) are progenitor cells located along the Bowman's capsule that can be isolated in urine, and display the capability to replace podocytes, but in certain situations cause glomerulosclerosis. In this study, a cohort of stable kidney transplant recipients with 6 months protocol biopsy was divided in two groups depending on the presence (uPEC+; n = 41) or absence (uPEC-; n = 25) of PECs in urine and followed for 2 years...
2018: Frontiers in Physiology
Laura Martinez Valenzuela, Juliana Draibe, Maria Quero Ramos, Xavier Fulladosa Oliveras, Edoardo Melilli, Josep Maria Cruzado Garrit, Juan Torras Ambrós
BACKGROUND: Calprotectin is produced by neutrophils and macrophages, and released during the acute phase of the ANCA vasculitis. The aim of our study was to determine if serum and urine calprotectin are disease activity and prognosis biomarkers in ANCA vasculitis patients during remission. METHODS: Forty-two ANCA vasculitis patients were included. Twenty-seven patients were in remission phase under immunosuppressive therapy, and 15 patients were in the acute phase...
2018: PloS One
Carmen R Silva-Correa, José L Cruzado-Razco, María V González-Blas, Juan M García-Armas, Segundo G Ruiz-Reyes, Víctor E Villarreal-La Torre, César D Gamarra-Sánchez
OBJECTIVE. : To identify and determine the phytoconstituent structure of Tessaria integrifolia Ruiz & Pav. leaves with leishmanicidal activity. MATERIALS AND METHODS.: Fluid extract of leaves was prepared, concentrated to soft extract, and used to evaluate leishmanicidal activity in Mesocricetus auratus with experimental leishmaniasis, at the dose of 250 mg/kg of soft extract by intramuscular route for 15 days. Extract was fractionated in 45 cm column chromatography with a 2...
April 2018: Revista Peruana de Medicina Experimental y Salud Pública
Marta Jarque, Edoardo Melilli, Elena Crespo, Anna Manonelles, Nuria Montero, Joan Torras, Josep M Cruzado, Sergi Luque, Salvador Gil-Vernet, Josep M Grinyó, Oriol Bestard
BACKGROUND: Whether CMV-specific cell-mediated immunity (CMI) at prophylaxis cessation predicts D+/R+ kidney transplants (KTR) at risk of late-onset CMV infection after receiving distinct induction therapies is still not well characterized. METHODS: We prospectively assessed CMV-specific CMI predicting late-onset CMV infection at prophylaxis withdrawal and at earlier time-points, in 96 consecutive D+/R+ patients receiving either antiinterleukin 2-receptor antibody (anti-IL2RA; n=50) or rabbit antithymoglobulin (rATG; n=46)...
August 20, 2018: Transplantation
Juliana Draibe, Xavier Rodó, Xavier Fulladosa, Laura Martínez-Valenzuela, Montserrat Diaz-Encarnación, Lara Santos, Helena Marco, Luis Quintana, Eva Rodriguez, Xoana Barros, Rosa Garcia, Anna Balius, Josep M Cruzado, Joan Torras
Background: The closure of long-standing gaps in our knowledge of aetiological factors behind anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a major challenge. Descriptive and analytical epidemiological studies can improve our understanding of environmental influences. Reported seasonal variations in AAV, mainly related to Wegener's disease, have shown an increasing number of cases in the winter months, which could be related to an extrinsic factor underlying infection...
August 2018: Clinical Kidney Journal
Michaela Jung, Bernhard Brüne, Andreas von Knethen, Roser Guiteras, Josep Maria Cruzado, Georgina Hotter, Anna Sola
Macrophage-epithelial cross-talk regulates cell cycle progression and represents an important factor in rescuing epithelial cells from cell cycle arrest in order to maintain a healthy epithelial phenotype. However, the underlying mechanisms are still not well defined. We provide evidence that macrophage-secreted lipocalin-2 (Lcn-2) plays a key role during this process. In a co-culture setup using cell cycle arrested NRK52e renal epithelial cells and primary bone marrow-derived macrophages, Lcn-2 restores proliferation through inhibition of peroxisome proliferator-activated receptor (PPAR)-γ...
November 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
Sergi Luque, Marc Lúcia, Edoardo Melilli, Carmen Lefaucheur, Marta Crespo, Alex Loupy, David Bernal, Montse Gomà, Marta Jarque, Elena Crespo, Núria Montero, Anna Manonelles, Josep M Cruzado, Salvador Gil-Vernet, Josep M Grinyó, Oriol Bestard
Antibody-mediated rejection (ABMR) is defined by specific histopathological lesions and evidence of circulating donor-specific antibodies (DSA). While DSA are not always detectable, monitoring donor-reactive memory B-cells (mBC) could recognize at-risk patients of developing ABMR. Peripheral donor-reactive mBC using a novel HLA B-cell ELISpot assay, serum DSA and numbers of different B-cell subsets were assessed in 175 consecutive kidney transplants undergoing either for-cause or 6 and 24-month surveillance biopsies for their association with main histological lesions of ABMR and impact on allograft outcome...
August 7, 2018: American Journal of Transplantation
José Tinoco-González, Mercedes Rubio-Manzanares-Dorado, Ana Senent-Boza, Virginia Durán-Muñoz-Cruzado, Luis Tallón-Aguilar, Felipe Pareja-Ciuró, Javier Padillo-Ruíz
OBJECTIVES: To analyze the clinical presentation, management, and outcome of acute appendicitis (AA) in pregnant and nonpregnant women of childbearing age. MATERIAL AND METHODS: Descriptive study of 2 cohorts of women (pregnant -P- and nonpregnant -NP-). The women, who were matched according to risk factors, were included when they underwent an emergency appendectomy based on clinical suspicion of AA. We recorded age, medical history, clinical presentation, management, and outcome...
2018: Emergencias: Revista de la Sociedad Española de Medicina de Emergencias
Maria Meneghini, Edoardo Melilli, Jaume Martorell, Ignacio Revuelta, Elisabet Rigol-Monzó, Anna Manonelles, Nuria Montero, David Cucchiari, Fritz Diekmann, Josep M Cruzado, Salvador Gil-Vernet, Josep M Grinyó, Oriol Bestard
Introduction: Despite the different assays available for immune-risk stratification before living-donor kidney transplantation (LDKT), the precise type and number of tests to perform remain uncertain. Methods: In a cohort of 330 consecutive LDKT patients, all of which were complement-dependent cytotoxicity (CDC)-crossmatch negative, we retrospectively analyzed the impact on main clinical outcomes of most sensitive immunoassays (complement-dependent cytotoxicity-panel-reactive antibody [CDC-PRA], flow cytometry crossmatch [FC-XM], donor-specific antibodies [DSAs], and their complement-binding capacity DSA-C3d]), together with donor/recipient HLA eplet matching...
July 2018: KI Reports
Edoardo Melilli, Anna Manonelles, Nuria Montero, Josep Grinyo, Alberto Martinez-Castelao, Oriol Bestard, Josep Cruzado
Arterial stiffness is a biologic process related to ageing and its relationship with cardiovascular risk is well established. Several methods are currently available for non-invasive measurement of arterial stiffness that provide valuable information to further assess patients' vascular status in real time. In kidney transplantation recipients, several factors could accelerate the stiffness process, such as the use of calcineurin inhibitors (CNIs), the presence of chronic kidney disease and other classical cardiovascular factors, which would explain, at least in part, the high cardiovascular mortality and morbidity...
June 2018: Clinical Kidney Journal
Miguel Hueso, Josep M Cruzado, Joan Torras, Estanislao Navarro
Atherosclerosis (ATH) and coronary artery disease (CAD) are chronic inflammatory diseases with an important genetic background; they derive from the cumulative effect of multiple common risk alleles, most of which are located in genomic noncoding regions. These complex diseases behave as nonlinear dynamical systems that show a high dependence on their initial conditions; thus, long-term predictions of disease progression are unreliable. One likely possibility is that the nonlinear nature of ATH could be dependent on nonlinear correlations in the structure of the human genome...
June 12, 2018: International Journal of Molecular Sciences
D Perez-Cruzado, A I Cuesta-Vargas
BACKGROUND: There are different instruments to measure energy expenditure in general population, but we do not know whether these instruments can be used in people with intellectual disability. METHODS: A sample of 33 participants was invited to wear a triaxial accelerometer during seven consecutive days. Energy expenditure was measured with the International Physical Activity Questionnaire Short Version (IPAQ-S) to gather data regarding the participant's descriptions of the physical activity performed...
August 2018: Journal of Intellectual Disability Research: JIDR
David Perez-Cruzado, Antonio Cuesta-Vargas, Elisa Vera-Garcia, Fermín Mayoral-Cleries
Anti-psychotic medication has emerged as the primary medical treatment for people with severe mental illness, despite the great risks involved in the use of this medication. In addition, this population suffers from problems of obesity, sedentary lifestyle and poor physical fitness, which is aggravated by the use of this type of medication. The objective of this study is to explore the influence of the most commonly used antipsychotics in this population (Olanzapine and Risperidone) on physical activity and the physical fitness of people with severe mental illness...
September 2018: Psychiatry Research
Nuria Montero, Alexandre Favà, Eva Rodriguez, Clara Barrios, Josep M Cruzado, Julio Pascual, Maria Jose Soler
BACKGROUND: Hepatitis C virus (HCV)-associated mixed cryoglobulinaemia is the manifestation of an inflammation of small and medium-sized vessels produced by a pathogenic IgM with rheumatoid factor activity generated by an expansion of B-cells. The immune complexes formed precipitate mainly in the skin, joints, kidneys or peripheral nerve fibres. Current therapeutic approaches are aimed at elimination of HCV infection, removal of cryoglobulins and also of the B-cell clonal expansions. The optimal treatment for it has not been established...
May 7, 2018: Cochrane Database of Systematic Reviews
Ariadna Arbiol-Roca, Ariadna Padró-Miquel, Anna Vidal-Alabró, Miquel Hueso, Pere Fontova, Oriol Bestard, Ines Rama, Joan Torras, Josep M Grinyó, Pedro Alía-Ramos, Josep Maria Cruzado, Nuria Lloberas
Cardiovascular disease is the leading cause of morbidity and mortality in kidney transplant recipients. Several single-nucleotide polymorphisms (SNPs) in the ANRIL gene pathway have been associated with cardiovascular events (CE). The main objective was to ascertain whether ANRIL (rs10757278) and CARD8 (rs2043211) SNPs could mediate susceptibility to CE. This was an observational follow-up cohort study of renal transplant recipients at Bellvitge University Hospital (Barcelona) from 2000 to 2014. A total of 505 recipients were followed up until achievement of a CE...
May 2, 2018: Transplant International: Official Journal of the European Society for Organ Transplantation
D Perez-Cruzado, A I Cuesta-Vargas, E Vera-Garcia, F Mayoral-Cleries
BACKGROUND: Quality of life of people with severe mental illness may be decrease by the high occurrence of metabolic and cardiovascular diseases. Physical fitness emerges as a modifying factor in this population through physical activity and this modification could influence in the quality of life of this population. The aim of the present study is to determine the contribution of physical fitness to the quality of life of people with severe mental illness. METHODS: In the current study, a physiotherapist and an occupational therapist assessed 62 people with severe mental illness...
May 2, 2018: Health and Quality of Life Outcomes
Kirill Grigorev, Sergey Kliver, Pavel Dobrynin, Aleksey Komissarov, Walter Wolfsberger, Ksenia Krasheninnikova, Yashira M Afanador-Hernández, Adam L Brandt, Liz A Paulino, Rosanna Carreras, Luis E Rodríguez, Adrell Núñez, Jessica R Brandt, Filipe Silva, J David Hernández-Martich, Audrey J Majeske, Agostinho Antunes, Alfred L Roca, Stephen J O'Brien, Juan Carlos Martínez-Cruzado, Taras K Oleksyk
Solenodons are insectivores that live in Hispaniola and Cuba. They form an isolated branch in the tree of placental mammals that are highly divergent from other eulipothyplan insectivores The history, unique biology, and adaptations of these enigmatic venomous species could be illuminated by the availability of genome data. However, a whole genome assembly for solenodons has not been previously performed, partially due to the difficulty in obtaining samples from the field. Island isolation and reduced numbers have likely resulted in high homozygosity within the Hispaniolan solenodon (Solenodon paradoxus)...
June 1, 2018: GigaScience
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