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Measurable residual disease

Jun Young Hong, Xiaoyu Zhang, Hening Lin
Sirtuins are a class of enzymes that utilize nicotinamide adenine dinucleotide, NAD+ , to remove various acyl groups from protein lysine residues. They have important biological functions and regulate numerous biological pathways. Small molecules that can modulate sirtuin enzymatic activities are potential therapeutic candidates to treat various human diseases. This protocol describes a high-performance liquid chromatography (HPLC)-based method to measure the enzyme kinetics for SIRT2 and SIRT6's demyristoylase activities and SIRT5's desuccinylase activity...
2018: Methods in Molecular Biology
Rukshana Shroff, Aysun Bayazit, Constantinos J Stefanidis, Varvara Askiti, Karolis Azukaitis, Nur Canpolat, Ayse Agbas, Ali Anarat, Bilal Aoun, Sevcan Bakkaloglu, Devina Bhowruth, Dagmara Borzych-Dużałka, Ipek Kaplan Bulut, Rainer Büscher, Claire Dempster, Ali Duzova, Sandra Habbig, Wesley Hayes, Shivram Hegde, Saoussen Krid, Christoph Licht, Mieczyslaw Litwin, Mark Mayes, Sevgi Mir, Rose Nemec, Lukasz Obrycki, Fabio Paglialonga, Stefano Picca, Bruno Ranchin, Charlotte Samaille, Mohan Shenoy, Manish Sinha, Colette Smith, Brankica Spasojevic, Enrico Vidal, Karel Vondrák, Alev Yilmaz, Ariane Zaloszyc, Michel Fischbach, Franz Schaefer, Claus Peter Schmitt
BACKGROUND: Cardiovascular disease is prevalent in children on dialysis and accounts for almost 30% of all deaths. Randomised trials in adults suggest that haemodiafiltration (HDF) with high convection volumes is associated with reduced cardiovascular mortality compared to high-flux haemodialysis (HD); however paediatric data are scarce. We designed the haemodiafiltration, heart and height (3H) study to test the hypothesis that children on HDF have an improved cardiovascular risk profile, growth and nutritional status and quality of life, compared to those on conventional HD...
August 10, 2018: BMC Nephrology
Donghui Liu, Liang Ji, Mingming Zhao, Yang Wang, Yansong Guo, Ling Li, Dongmei Zhang, Liang Xu, Bing Pan, Jinzi Su, Song Xiang, Subramaniam Pennathur, Jingxuan Li, Jianing Gao, Pingsheng Liu, Belinda Willard, Lemin Zheng
Apolipoprotein A-I (apoA-I), the major protein compontent of high-density lipoprotein (HDL), exerts many anti-atherogenic functions. This study aimed to reveal whether nonenzymatic glycation of specific sites of apoA-I impaired its anti-inflammatory effects in type 2 diabetes mellitus (T2DM). LC-MS/MS was used to analyze the specific sites and the extent of apoA-I glycation either modified by glucose in vitro or isolated from T2DM patients. Cytokine release in THP-1 monocyte-derived macrophages was tested by ELISA...
August 6, 2018: Journal of Molecular and Cellular Cardiology
Simona Lattanzi, Francesco Brigo, Eugen Trinka, Claudia Cagnetti, Mario Di Napoli, Mauro Silvestrini
Spontaneous intracerebral hemorrhage (ICH) accounts for approximately 10 to 30% of all acute cerebrovascular events, and it is the type of stroke associated with the highest rates of mortality and residual disability. The inflammatory response is early triggered by hematoma components and can enhance the damage within the hemorrhagic brain. Assessment of peripheral biomarkers of inflammation could contribute to increase knowledge about some of the mechanisms involved in the ICH-induced injury and yield information on the disease course...
August 8, 2018: Translational Stroke Research
Mhd Firas Safadi
Background: Qat-induced intestinal obstruction is an acute manifestation of qat chewing which was not described previously in the literature. The aim of this case series is to describe the clinical presentation, the diagnostic and therapeutic measures, and the course of the disease. Methods: This retrospective case series included all patients who presented with qat-induced intestinal obstruction between July 1, 2013, and December 31, 2015. Results: The study included seven patients (five males and two females)...
July 2018: Avicenna Journal of Medicine
Bhavna Chawla, Shweta Chaurasia, Sanjay Sharma, Rajesh Pattebahadur, Fahmi Hasan, Rachna Seth, Seema Kashyap, Seema Sen
PURPOSE: Extraocular retinoblastoma with optic nerve invasion is treated by a multimodal protocol consisting of neoadjuvant chemotherapy, enucleation, and adjuvant therapy. This study was conducted to evaluate the performance of magnetic resonance imaging (MRI) used for tumor restaging in these children after systemic chemotherapy administration. METHODS: Contrast-enhanced MRI scan of orbits and brain was performed at diagnosis and patients were treated with neoadjuvant chemotherapy...
August 8, 2018: Ophthalmic Genetics
Vincent Delatour, Noemie Clouet-Foraison, François Gaie-Levrel, Santica M Marcovina, Andrew N Hoofnagle, Zsuzsanna Kuklenyik, Michael P Caulfield, James D Otvos, Ronald M Krauss, Krishnaji R Kulkarni, John H Contois, Alan T Remaley, Hubert W Vesper, Christa M Cobbaert, Philippe Gillery
BACKGROUND: Despite the usefulness of standard lipid parameters for cardiovascular disease risk assessment, undiagnosed residual risk remains high. Advanced lipoprotein testing (ALT) was developed to provide physicians with more predictive diagnostic tools. ALT methods separate and/or measure lipoproteins according to different parameters such as size, density, charge, or content, and equivalence of results across methods has not been demonstrated. METHODS: Through a split-sample study, 25 clinical specimens (CSs) were assayed in 10 laboratories before and after freezing using the major ALT methods for non-HDL particles (non-HDL-P) or apolipoprotein B-100 (apoB-100) measurements with the intent to assess their comparability in the current state of the art...
August 7, 2018: Clinical Chemistry
Dario Baratti, Shigeki Kusamura, Domenico Iusco, Christian Cotsoglou, Marcello Guaglio, Luigi Battaglia, Salvatore Virzì, Vincenzo Mazzaferro, Marcello Deraco
BACKGROUND: Survival improvements have been reported in selected patients affected by colorectal peritoneal metastases who were undergoing cytoreductive surgery with intraperitoneal hyperthermic chemotherapy. Treatment of peritoneal metastases associated with extraperitoneal disease is still controversial. OBJECTIVE: We assessed the prognostic impact of a history of extraperitoneal disease that was curatively treated either at the same time as or before the onset of peritoneal metastases...
September 2018: Diseases of the Colon and Rectum
Pengcheng Nie, Tao Dong, Shupei Xiao, Lei Lin, Yong He, Fangfang Qu
Thiabendazole (TBZ) is widely used in sclerotium blight, downy mildew as well as root rot disease prevention and treatment in plant. The indiscriminate use of TBZ causes the excess pesticide residues in soil, which leads to soil hardening and environmental pollution. Therefore, it is important to accurately monitor whether the TBZ residue in soil exceeds the standard. For this study, density functional theory (DFT) was used to theoretically analyze the molecular structure of TBZ, gold nanoparticles (AuNPs) were used to enhance the detection signal of surface-enhanced Raman spectroscopy (SERS) and the TBZ residue in red soil extracts was quantitatively determined by SERS...
August 5, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Antonio Daniele Pinna, Tian Yang, Vincenzo Mazzaferro, Luciano De Carlis, Jian Zhou, Sasan Roayaie, Feng Shen, Carlo Sposito, Matteo Cescon, Stefano Di Sandro, He Yi-Feng, Philip Johnson, Alessandro Cucchetti
OBJECTIVE: The aim of this study was to estimate probabilities of achieving the statistical cure from hepatocellular carcinoma (HCC) with hepatic resection (HR) and liver transplantation (LT). BACKGROUND: Statistical cure occurs when the mortality of a specific population returns to values of that of general population. Resection and transplantation are considered potentially curative therapies for HCC, but their effect on the residual entire life-expectancy has never been investigated...
August 3, 2018: Annals of Surgery
Alisdair M Boag, Michael R Christie, Kerry A McLaughlin, Harriet M Syme, Peter Graham, Brian Catchpole
Autoantibodies directed against the P450 side chain cleavage enzyme (P450scc) have been recently described in dogs affected with hypoadrenocorticism, consistent with an immune-mediated pathogenesis of this endocrinopathy. In human autoimmune Addison's disease, autoantibodies may have a predictive value, being detectable before clinical signs developing, and have been shown to persist for a period of time after diagnosis. Furthermore, an autoantibody positive status post-diagnosis has been associated with successful remission of Addison's disease following B-cell depletion, suggesting active immunopathology in these cases...
August 2018: Veterinary Immunology and Immunopathology
Ryuji Sakakibara
PURPOSE: Multiple sclerosis (MS), neuromyelitis optica (NMO), and related disorders are major immune-mediated central nervous system diseases affecting the spinal cord. We reviewed the occurrence of neurogenic lower urinary tract dysfunction (NLUTD) in these categories of diseases. METHODS: We systematically reviewed the literature regarding bladder dysfunction in MS, NMO spectrum disorder (NMOSD), and related disorders (acute disseminated encephalomyelitis, acute immune-mediated myelopathy, and meningitis-retention syndrome)...
August 3, 2018: Clinical Autonomic Research: Official Journal of the Clinical Autonomic Research Society
Xiao-Dong Mo, Yu Wang, Xiao-Hui Zhang, Lan-Ping Xu, Chen-Hua Yan, Huan Chen, Yu-Hong Chen, Ya-Zhen Qin, Kai-Yan Liu, Xiao-Jun Huang
BACKGROUND: RUNX1-RUNX1T1 transcript levels were established as a powerful marker for predicting relapse in patients with t(8;21) acute myeloid leukemia (AML). We aimed to identify the efficacy of minimal residual disease (MRD)-directed interferon-alpha (IFN-α) treatment in patients with t(8;21) AML who were positive for MRD after allogeneic hematopoietic stem cell transplantation (allo-HSCT; n =42). SUBJECTS, MATERIALS, AND METHODS: MRD-positive status was defined as a <4...
August 3, 2018: Oncologist
Niina Matikainen, Sanni Söderlund, Elias Björnson, Kirsi Pietiläinen, Antti Hakkarainen, Nina Lundbom, Marja-Riitta Taskinen, Jan Borén
AIMS/HYPOTHESIS: Subjects with type 2 diabetes and non-alcoholic fatty liver disease (NAFLD) exhibit considerable residual risk for cardiovascular disease (CVD). There is therefore increasing interest in targeting postprandial lipid metabolism and remnant cholesterol. Treatment with the glucagon-like peptide 1 (GLP-1) analogue liraglutide reduces CVD risk by partly unexplained mechanisms. Here we investigated the effects of liraglutide intervention on ectopic fat depots, hepatic lipogenesis and fat oxidation, postprandial lipid metabolism and glycaemia in humans with type 2 diabetes...
August 2, 2018: Diabetes, Obesity & Metabolism
Michael Nshanian, Carter Lantz, Piriya Wongkongkathep, Thomas Schrader, Frank-Gerrit Klärner, Anika Blümke, Clément Despres, Michael Ehrmann, Caroline Smet-Nocca, Gal Bitan, Joseph A Loo
Native top-down mass spectrometry (MS) and ion mobility spectrometry (IMS) were applied to characterize the interaction of a molecular tweezer assembly modulator, CLR01, with tau, a protein believed to be involved in a number of neurodegenerative disorders, including Alzheimer's disease. The tweezer CLR01 has been shown to inhibit aggregation of amyloidogenic polypeptides without toxic side effects. ESI-MS spectra for different forms of tau protein (full-length, fragments, phosphorylated, etc.) in the presence of CLR01 indicate a primary binding stoichiometry of 1:1...
July 30, 2018: Journal of the American Society for Mass Spectrometry
Jennifer T Saville, Belinda K McDermott, Janice M Fletcher, Maria Fuller
PURPOSE: Expanding treatments for the mucopolysaccharidoses-a family of genetic disorders-place unprecedented demands for accurate, timely diagnosis because best outcomes are seen with early initiation of appropriate therapies. Here we sought to improve the diagnostic odyssey by measuring specific glycosaminoglycan fragments with terminal residues complicit with the genetic defect resulting in precise diagnosis rather than the usual first-line, ambiguous total glycosaminoglycan determinations that return poor diagnostic yield...
July 31, 2018: Genetics in Medicine: Official Journal of the American College of Medical Genetics
Marie Viala, Akiko Chiba, Simon Thezenas, Laure Delmond, Pierre-Jean Lamy, Sarah L Mott, Mary C Schroeder, Alexandra Thomas, William Jacot
BACKGROUND: There has been interest in the potential benefit of vitamin D (VD) to improve breast cancer outcomes. Pre-clinical studies suggest VD enhances chemotherapy-induced cell death. Vitamin D deficiency was associated with not attaining a pathologic complete response (pCR) following neoadjuvant chemotherapy (NAC) for operable breast cancer. We report the impact of VD on pCR and survival in an expanded cohort. METHODS: Patients from Iowa and Montpellier registries who had serum VD level measured before or during NAC were included...
July 30, 2018: BMC Cancer
Faysal A Yafi, Linley Diao, Kenneth J DeLay, Ling DeYoung, Riad Talib, Laith Alzweri, Gerald Brock, Wayne J G Hellstrom, Georgios Hatzichristodoulou
OBJECTIVE: To compare in a prospective, non-randomized fashion the outcomes of Collagenase Clostridium Histolyticum (CCH) and surgical interventions (tunical plication [TP] and partial plaque excision and grafting [PEG]) in patients with PD. Intralesional injection of CCH is the only Food and Drug Administration-approved non-surgical intervention for Peyronie's disease (PD). MATERIALS AND METHODS: Between 6/2015 and 1/2017, 57 patients with PD and dorsal, dorsolateral and/or lateral penile curvatures 30-90 degrees were included in this study...
July 27, 2018: Urology
Li Zhang, Qi Zhou
Phase III trials have shown improved survival in ovarian cancer patients when the anti-vascular endothelial growth factor (VEGF) therapy bevacizumab is added to first-line chemotherapy. However, further evidence is needed regarding bevacizumab when used with dose-dense paclitaxel/carboplatin chemotherapy in advanced ovarian cancer patients. This single-arm trial enrolled 184 advanced-stage (III-IV) epithelial ovarian cancer patients following primary debulking. Enrollees were treated with dose-dense paclitaxel/carboplatin chemotherapy with bevacizumab administered on the first day of cycles 2 through 6...
July 27, 2018: European Journal of Pharmacology
Stepan Hrabovsky, Frantisek Folber, Jan M Horacek, Olga Stehlikova, Hana Jelinkova, Cyril Salek, Michael Doubek
BACKGROUND: Minimal residual disease (MRD) is an important prognostic maker in acute lymphoblastic leukemia (ALL). However, few data comparing the measurement of adult ALL MRD using different methods in daily practice are available. We conducted an analysis comparing the importance of flow cytometry (FCM) and real-time quantitative polymerase chain reaction (PCR) in the assessment of MRD in adult ALL. PATIENTS AND METHODS: Fifty-six consecutive adult patients with both Philadelphia-negative and -positive ALL treated according to an intensive protocol were enrolled in the study...
July 4, 2018: Clinical Lymphoma, Myeloma & Leukemia
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