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https://www.readbyqxmd.com/read/30551230/distribution-of-late-onset-neonatal-sepsis-pathogens-differs-in-inpatient-and-outpatient-settings
#1
Celeste G Black, Larissa Tavares, Anna Stachel, Adam J Ratner, Tara M Randis
OBJECTIVE:  We sought to examine pathogen distribution and clinical presentation of late-onset sepsis (LOS) at an urban tertiary care center. STUDY DESIGN:  We performed a retrospective review of all culture-confirmed cases of LOS presenting to our institution from 2013 to 2017. Medical records were evaluated for demographic information, sepsis risk factors, encounter location, and clinical outcome. RESULTS:  We identified 97 cases of LOS, with a median age at diagnosis of 25 days...
December 14, 2018: American Journal of Perinatology
https://www.readbyqxmd.com/read/30550945/etiology-and-epidemiology-of-community-acquired-pneumonia-in-adults-requiring-hospital-admission-a-prospective-study-in-rural-central-philippines
#2
Socorro Lupisan, Akira Suzuki, Noel Macalalad, Rutchie Egos, Lydia Sombrero, Michiko Okamoto, Clyde Dapat, Melisa Mondoy, Hazel Galang, Vicente Francisco Froilan Zeta, Flora de la Pena, Vicente Romano, Remigio Olveda, Hitoshi Oshitani
BACKGROUND: Community-acquired pneumonia (CAP) is a common cause of morbidity and mortality among adults worldwide. However, the distribution of etiology of CAP varies from one country to another with limited data from rural areas. METHODS: A prospective hospital-based study on adult CAP was conducted in Leyte, Central Philippines from May 2010 to May 2012. Blood, sputum, and nasopharyngeal samples obtained from patients were used to identify pathogens using standard microbiological culture methods and PCR...
December 11, 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/30550595/genome-survey-sequencing-for-the-characterization-of-genetic-background-of-dracaena-cambodiana-and-its-defense-response-during-dragon-s-blood-formation
#3
Xupo Ding, Wenli Mei, Shengzhuo Huang, Hui Wang, Jiahong Zhu, Wei Hu, Zehong Ding, Weiwei Tie, Shiqing Peng, Haofu Dai
Dragon's blood collected from the genus Dracaena is used as a renowned traditional medicine in various cultures worldwide. However, the genetics of the genus Dracaena and the formation mechanism of dragon's blood remain poorly understood. Here, we generate the first draft genome reference assembly of an elite Chinese Dracaena species, Dracaena cambodiana, from next-generation sequencing data with 89.46× coverage. The reads were assembled into 2,640,704 contigs with an N50 length of 1.87 kb, and a 1.05 Gb assembly was finally assembled with 2,379,659 scaffolds...
2018: PloS One
https://www.readbyqxmd.com/read/30550234/comparison-of-pcr-fluorescent-in-situ-hybridization-and-blood-cultures-for-detection-of-bacteremia-in-children-and-adolescents-during-antibiotic-therapy
#4
Tomasz W Źródłowski, Danuta Jurkiewicz-Badacz, Agnieszka Sroka-Oleksiak, Dominika Salamon, Małgorzata Bulanda, Tomasz Gosiewski
The gold standard in microbiological diagnostics of bacteremia is a blood culture in automated systems. This method may take several days and has low sensitivity. New screening methods that could quickly reveal the presence of bacteria would be extremely useful. The objective of this study was to estimate the effectiveness of these methods with respect to blood cultures in the context of antibiotic therapy. Blood samples from 92 children with sepsis were analyzed. Blood cultures were carried out in standard automated systems...
2018: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/30549995/direct-ms-based-bacterial-identification-of-blood-cultures-comparison-of-an-in-house-tritonx-protocol-with-bruker-sepsityper
#5
Maximilian Kittel, Anna Greulich, Peter Findeisen, Verena Haselmann, Achim Burrer, Christian Willem, Thomas Miethke, Parviz Ahmad-Nejad, Michael Neumaier
No abstract text is available yet for this article.
October 31, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/30549244/cell-templated-silica-microparticles-with-supported-lipid-bilayers-as-artificial-antigen-presenting-cells-for-t-cell-activation
#6
Brynn R Olden, Caleb R Perez, Ashley L Wilson, Ian I Cardle, Yu-Shen Lin, Bryan Kaehr, Joshua A Gustafson, Michael C Jensen, Suzie H Pun
Biomaterial properties that modulate T cell activation, growth, and differentiation are of significant interest in the field of cellular immunotherapy manufacturing. In this work, a new platform technology that allows for the modulation of various activation particle design parameters important for polyclonal T cell activation is presented. Artificial antigen presenting cells (aAPCs) are successfully created using supported lipid bilayers on various cell-templated silica microparticles with defined membrane fluidity and stimulating antibody density...
December 13, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/30549135/human-native-endocarditis-caused-by-streptococcus-canis-a-case-report
#7
Barbora Mališová, Petr Šantavý, Yvona Lovečková, Bořivoj Hladký, Iva Kotásková, Jiří Pol, Vladimír Lonský, Petr Němec, Tomáš Freiberger
We report a very rare case of Streptococcus canis native infective endocarditis in a 73-year-old woman living in close contact with her dog. Her echocardiography showed large calcifications in the mitral annulus, massive regurgitation below the posterior leaflet, and adjacent vegetation. Blood culture was positive for Streptococcus Lancefield group G. A coronary artery bypass and mitral valve replacement had to be done. Streptococcus canis was detected in a heart valve using a broad range PCR followed by 16S rRNA and confirmed by tuf gene sequencing, while tissue culture remained negative...
January 2019: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30548621/recipient-sepsis-caused-by-lactococcus-garvieae-contamination-of-platelets-from-a-donor-with-colon-cancer
#8
Shuichi Nakamura, Kenji Nakai, Momoko Sakata, Yoshio Nagaoka, Kozue Yoshida, Uichiro Katsumata, Toshimi Chiba, Keiji Matsubayashi
Lactococcus garvieae is a well-known fish pathogen that has low virulence in humans and is rarely isolated from the blood cultures of endocarditis patients. We describe herein the first reported case of transfusion-transmitted L. garvieae sepsis caused by a contaminated platelet concentrate from a donor who consumed raw octopus before blood donation. Retrospective examination of the laboratory results of the index donor revealed that his haemoglobin levels had been steadily decreasing, which led to the detection of a latent colon cancer...
December 12, 2018: Vox Sanguinis
https://www.readbyqxmd.com/read/30547754/leptotrichia-trevisanii-bacteremia-in-a-woman-with-systemic-lupus-erythematosus-receiving-high-dose-chemotherapy
#9
Hongyan Hou, Zhongju Chen, Lei Tian, Ziyong Sun
BACKGROUND: Leptotrichia species are aerotolerant, Gram-negative fusiform bacteria. Cases of bacteremia caused by Leptotrichia trevisanii in immunocompromised patients have been rarely reported. CASE PRESENTATION: A 33-year-old female with systemic lupus erythematosus (SLE) was admitted to the department of rheumatology with bleeding from a mucosal ulcer. One month previously, she had visited our hospital and begun to receive methotrexate therapy, but mis-dosed for nearly 1 month at home...
December 14, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/30547196/siu-icud-consultation-on-bladder-cancer-basic-science
#10
David J McConkey, Seth P Lerner
PURPOSE: To provide a condensed summary of the Basic Science chapter that was included in the Third International Consultation on Bladder Cancer. METHODS: World bladder cancer basic science experts used the published literature to create summaries of recent progress in their areas of expertise. RESULTS: The completion of several large-scale genomics projects coupled with a strong collaborative culture within the research community and the exciting clinical activity of immune checkpoint blockade have combined to transform the bladder cancer research landscape...
December 13, 2018: World Journal of Urology
https://www.readbyqxmd.com/read/30546901/levels-of-regulatory-t-cells-and-invariant-natural-killer-cells-and-their-associations-with-regulatory-b-cells-in-patients-with-non-hodgkin-lymphoma
#11
Mohamed-Rachid Boulassel, Zahra Al Qarni, Ikram Burney, Hammad Khan, Abeer Al-Zubaidi, Amal Al Naamani, Huda Al-Hinai, Amira Al-Badi, Rizwan Nabi Qureshi, Vinodh Panjwani, Khalil Al Farsi
Due to their immunoregulatory properties, several specialized cell subsets, including regulatory T (Treg), invariant natural killer T (iNKT) and regulatory B (Breg) cells, are involved in the pathogenesis of non-Hodgkin lymphoma (NHL). However, the interaction between various cells remains to be elucidated. The aim of the present study was to evaluate the levels of Treg, iNKT and Breg cell subsets and their interrelationships in the peripheral blood (PB) and bone marrow (BM) of patients with B-cell NHL who received rituximab-based regimens and achieved a complete remission...
December 2018: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/30546392/human-umbilical-cord-derived-mesenchymal-stem-cells-ameliorate-the-enteropathy-of-food-allergies-in-mice
#12
Nannan Yan, Jie Xu, Chuanxiang Zhao, Yi Wu, Fengwei Gao, Ci Li, Wenhui Zhou, Tengfei Xiao, Xiaoming Zhou, Qixiang Shao, Sheng Xia
Food allergy prevalence has steadily increased worldwide over the past decades and immunotherapeutic treatment strategies are gaining attention. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) exhibit similar immune regulatory properties to bone marrow-derived MSCs. hUC-MCSs can be prepared with fewer ethical constraints and are potential candidates for allergic disorder therapies. The current study aimed to investigate potential antiallergic properties of hUC-MSCs in mice with ovalbumin (OVA)-induced food allergy...
December 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30544476/incidence-and-clinical-features-of-patients-with-peritoneal-dialysis-peritonitis-complicated-by-bacteremia
#13
Chang-Chih Tsai, Chien-Chin Hsu, Kuo-Tai Chen
The standard treatment of peritoneal dialysis peritonitis (PD peritonitis) is intraperitoneal antibiotic therapy. In patients with PD peritonitis complicated by bacteremia, intraperitoneal antibiotics combined with elective removal of the infected intraperitoneal catheter may be inadequate.We collected data of all patients with PD peritonitis admitted to Chi-Mei Medical Center during a 4-year period. We reviewed the medical records of the study cohort and collected their in-hospital details. Patients with positive blood culture results were assigned to the bacteremia group, whereas those with negative blood culture results were assigned to the peritonitis-only group...
December 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/30544442/emergence-of-daptomycin-non-susceptible-coagulase-negative-staphylococci-in-patients-with-cardiovascular-device-infections-two-cases-report-investigated-by-whole-genome-analysis
#14
Hideharu Hagiya, Yo Sugawara, Keigo Kimura, Shigeto Hamaguchi, Isao Nishi, Masahiro Hayashi, Yukihiro Akeda, Kazunori Tomono
RATIONALE: Daptomycin (DAP) is a key drug for treating severe Staphylococcus infections. The emergence of DAP non-susceptible Staphylococcus aureus has been widely recognized in clinical situations, although the clinical status of DAP non-susceptible coagulase-negative Staphylococcus (CoNS) infections is unclear. We encountered 2 cases of cardiovascular device infections that were associated with DAP non-susceptible CoNS. PATIENT CONCERNS: The first case involved a 60-year-old woman with a pump pocket infection in a left ventricular assist device...
December 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/30544238/eimeria-challenge-adversely-affected-long-bone-attributes-linked-to-increased-resorption-in-14-day-old-broiler-chickens
#15
R Akbari Moghaddam Kakhki, Z Lu, A Thanabalan, H Leung, M Mohammadigheisar, E Kiarie
There is limited information on the effects of enteric pathogen on bone quality in rapidly growing broiler chicks. We examined tibia and femur attributes (length, diameter, relative weight of ash content [AC] to the BW, ash concentration [AP]) and serum bone-turnover markers including receptor activator of nuclear factor kappa-B ligand (RANKL) for resorption, alkaline phosphatase (ALP) for mineralization, and selected serum metabolites in 14-day-old broilers challenged with Eimeria. A total of 160 (80 males and 80 females) 1-day-old Ross × Ross 708 chicks were used...
December 13, 2018: Poultry Science
https://www.readbyqxmd.com/read/30544178/reporting-and-categorization-of-blood-culture-contaminants-in-infants-and-young-children-a-scoping-review
#16
Laura Chappell-Campbell, Hayden T Schwenk, Nicole Capdarest-Arest, Alan R Schroeder
Background: Blood cultures are obtained routinely for infants and young children for the evaluation for serious bacterial infection. Isolation of organisms that represent possible contaminants poses a management challenge. The prevalence of bacteremia reported in this population is potentially biased by inconsistent contaminant categorization reported in the literature. Our aim was to systematically review the definition and reporting of contaminants within the literature regarding infant bacteremia...
December 13, 2018: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/30543695/rapid-antibiotic-susceptibility-testing-from-blood-culture-bottles-with-species-agnostic-real-time-polymerase-chain-reaction
#17
Tucker Maxson, Candace D Blancett, Amanda S Graham, Christopher P Stefan, Timothy D Minogue
Development and implementation of rapid antimicrobial susceptibility testing is critical for guiding patient care and improving clinical outcomes, especially in cases of sepsis. One approach to reduce the time-to-answer for antimicrobial susceptibility is monitoring the inhibition of DNA production, as differences in DNA concentrations are more quickly impacted compared to optical density changes in traditional antimicrobial susceptibility testing. Here, we use real-time PCR to rapidly determine antimicrobial susceptibility after short incubations with antibiotic...
2018: PloS One
https://www.readbyqxmd.com/read/30543501/differences-in-microrna-expression-profile-between-vastus-lateralis-samples-and-myotubes-in-copd-cachexia
#18
Esther Barreiro, Antonio Sancho-Muñoz, Ester Puig-Vilanova, Anna Salazar-Degracia, Sergi Pascual-Guardia, Carme Casadevall, Joaquim Gea
Quadriceps muscle weakness and wasting are common comorbidities in COPD. MicroRNA expression upregulation may favor muscle mass growth and differentiation. We hypothesized whether the profile of muscle-enriched microRNAs in cultured myotubes differs between COPD patients of a wide range of body composition and healthy controls and whether expression levels of those microRNAs from COPD and controls differ between in vivo and in vitro conditions. Twenty-nine COPD patients (n=15 with muscle wasting, FFMI 15 kg/m2 and n=14 with normal body composition, FFMI 18 kg/m2 ) and 10 healthy controls (FFMI, 19 kg/m2 ) were consecutively recruited...
December 13, 2018: Journal of Applied Physiology
https://www.readbyqxmd.com/read/30542398/antimicrobial-resistance-in-bacterial-pathogens-among-hospitalised-patients-with-severe-invasive-infections
#19
Ovidiu Zlatian, Andrei Theodor Balasoiu, Maria Balasoiu, Oana Cristea, Anca Oana Docea, Radu Mitrut, Demetrios A Spandidos, Aristides M Tsatsakis, Gabriela Bancescu, Daniela Calina
The most severe infections are invasive infections, due to the fact that the germs can accumulate in multiple sites and produce a body-wide infection known as sepsis. Septic shock has the highest mortality rate among non-traumatic medical conditions. In this study, we aimed to evaluate the incidence and prevalence of invasive infections in a hospital environment. Another second objective was to establish the aetiology of invasive infections in our hospital and the antibiotic resistance profile of the germs involved, which are both important for determining the therapeutic approach for the treatment of these infections...
December 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30542386/the-expression-level-of-mir-155-in-plasma-and-peripheral-blood-mononuclear-cells-in-coronary-artery-disease-patients-and-the-associations-of-these-levels-with-the-apoptosis-rate-of-peripheral-blood-mononuclear-cells
#20
Duo Zhao, Jing Zhao, Jiangbin Sun, Yanling Su, Jinfeng Jian, Huaan Ye, Jiawang Lin, Zongda Yang, Jiatao Feng, Zhiping Wang
The aim of the study was to investigate the expression of miR-155 in plasma and peripheral blood mononuclear cells (PBMCs), the effects of miR-155 on the apoptosis rate of PBMCs and the extent of coronary stenosis in coronary artery disease patients. Seventy chest pain patients were divided into three groups by symptoms, signs, auxiliary examination and coronary arteriography: 21 cases in the acute myocardial infarction group (AMI), 23 cases in the Angor pectoris group (AP) and 26 cases in the control group (CT)...
December 2018: Experimental and Therapeutic Medicine
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