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https://www.readbyqxmd.com/read/30328721/immunoexpression-of-aquaporins-1-2-3-and-4-in-kidney-of-yak-bos-grunniens-on-the-qinghai-tibetan-plateau
#1
Y P Ding, H S Yu, J L Wang, B P Shao
Aquaporins (AQP) 1, 2, 3 and 4 belong to the aquaporin water channel family and play an important role in urine concentration by reabsorption of water from renal tubule fluid. Renal AQPs have not been reported in the yak (Bos grunniens), which resides in the Qinghai Tibetan Plateau. We investigated AQPs 1-4 expressions in the kidneys of Yak using immunohistochemical staining. AQP1 was expressed mainly in the basolateral and apical membranes of the proximal tubules and descending thin limb of the loop of Henle...
October 17, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/30304826/comprehensive-analysis-of-microrna%C3%A2-messenger-rna-from-white-yak-testis-reveals-the-differentially-expressed-molecules-involved-in-development-and-reproduction
#2
Quanwei Zhang, Qi Wang, Yong Zhang, Shuru Cheng, Junjie Hu, Youji Ma, Xingxu Zhao
Testis development is a vital and tightly regulated process in mammals. Understanding the biological mechanisms underlying testis development will benefit the animal reproduction industry. Expression changes in microRNA and messenger RNA in response to dynamic regulation effects have been associated with this process. However, very little is known about the roles of these molecules in yak development. Using whole-genome small RNA and messenger RNA sequencing, we performed a comprehensive analysis of the microRNA⁻messenger RNA interaction network expression in the testicles of Tianzhu white yaks during three developmental stages...
October 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30268611/short-communication-characterization-of-gene-expression-profiles-related-to-yak-milk-protein-synthesis-during-the-lactation-cycle
#3
Wei Xia, Johan S Osorio, Yuanxiao Yang, DongLiang Liu, Ming Feng Jiang
This research assessed the gene expression patterns related to the synthesis of milk in yak, which is characterized by high fat and protein content but low yield. The yak (Bos grunniens) is one of the most crucial domestic animals in Tibetan life; however, the genetic and molecular factors underlying yak milk protein synthesis remain understudied. Yak mammary biopsies harvested during late-pregnancy (d -15) through the end of subsequent lactation (d 1, 15, 30, 60, 180, and 240) were used to evaluate gene expression via real-time quantitative PCR...
September 26, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/30260685/microrna-125b-regulates-apoptosis-by-targeting-bone-morphogenetic-protein-receptor-1b-in-yak-granulosa-cells
#4
Yilong Yao, Jiaqiang Niu, Suolang Sizhu, Bojiang Li, Yun Chen, Rongyang Li, Qiangba Yangzong, Qifa Li, Yefen Xu
The intronic microRNA, miR-125b, plays a vital role in promyelocytic and hematopoietic stem cells, and in the development and apoptosis of cancer cells. In this study, we showed that miR-125b regulates granulosa cell (GC) apoptosis in the yak ovary. Bioinformatic analyses and luciferase reporter assays demonstrated that bone morphogenetic protein receptor type 1B (BMPR1B) is an miR-125b target. miR-125b overexpression induced apoptosis in yak GC, and affected the mRNA and protein expression of BMPR1B and the ratio of Bcl2/Bax...
September 27, 2018: DNA and Cell Biology
https://www.readbyqxmd.com/read/30254613/prevalence-risk-factors-and-genotypes-of-toxoplasma-gondii-in-food-animals-and-humans-2000-2017-from-china
#5
Hui Dong, Ruijing Su, Yaoyao Lu, Mengyao Wang, Jing Liu, Fuchun Jian, Yurong Yang
Toxoplasma gondii as a food-borne pathogen, the infection of it in food animals has relation with human toxoplasmosis, but the trends and epidemiological features of T. gondii infections in food animals are rarely studied in China. The aimed of this study was to assess the epidemiology and risks of T. gondii in sheep, goats, swines, chickens, yaks, cattle and humans from 2000 to 2017 and to explore prevention and control strategies. The overall seroprevalence of T. gondii infections in food animals is 23.7% (39,194/165,417, 95%CI, 23...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30250374/seasonal-changes-of-rumen-and-intestine-morphology-of-the-qinghai-yak-bos-grunniens
#6
Bao A Ding, Shuang Q Ma, Zong R Li, Xi L Li, Stephen R Madigosky
Aim: The aim of the current study was to investigate the effects of seasonal changes in grass quality on the ruminal and intestinal morphology of male Qinghai yaks. Materials and Methods: A total of four male yaks with the same age of 4 years old from each season (summer and winter) were randomly selected and slaughtered to determine the effect of different season on intestinal morphology of yak in the Qinghai-Tibetan Plateau. Results: The histological analysis shows that male yak has the longer and wider papillae in rumen in green season...
August 2018: Veterinary World
https://www.readbyqxmd.com/read/30229981/identification-and-diversity-of-y-chromosome-haplotypes-in-qinghai-yak-populations
#7
Z J Ma, X T Xia, S M Chen, X C Zhao, L L Zeng, Y L Xie, S Y Chao, J T Xu, Y G Sun, R Z Li, Z X Guanque, J L Han, C Z Lei
The aim of the present study was to perform a preliminary analysis of the characterization and diversity of Y-chromosome haplotypes/haplogroups in yak of Qinghai Province, China. A total of 322 male yaks from nine populations belonging to three officially recognized breeds (Gaoyuan, Huanhu and Datong) were sampled. Animals were genotyped using six previously reported Y-SNPs present in the SRY, USP9Y, UTY, AMELY and OFD1Y genes and four new Y-SNPs in the OFD1Y gene (g.569A>C, g.578A>C, g.608G>T and g...
September 19, 2018: Animal Genetics
https://www.readbyqxmd.com/read/30192403/further-evidence-for-sustainable-alternatives-to-replace-threatened-animal-horn-based-on-quantitative-proteomic-analysis
#8
Rui Liu, Qiong Huang, Zhenhua Zhu, Yan Zhang, Dawei Qian, Jin-Ao Duan
In the present study, a quantitative proteomic approach was used to analyze and compare the proteome in horns from endangered species (rhinoceros, Saiga antelope, and Tibetan antelope) and common species (yak, water buffalo, and goat) based on the isobaric tag for relative and absolute quantification techniques. In total, 591 proteins were identified, and 321 were quantified and categorized based on molecular function, cellular component, and biological process. Principal component analysis and hierarchical clustering analysis results based on differences in the amount of protein identified three major clusters, and proteins including transglutaminase, desmocollin, and elongation factors were selected as trait components from proteomic patterns of horn samples from different species...
September 7, 2018: Electrophoresis
https://www.readbyqxmd.com/read/30178622/molecular-cloning-and-expression-of-ghrelin-in-the-hypothalamus-pituitary-gastrointestinal-tract-axis-of-the-yak-bos-grunniens-in-the-qinghai-tibetan-plateau
#9
Yanping Ding, Na Zhang, Jialong Li, Yiran Jin, Baoping Shao
Ghrelin is a very important brain-gut peptide that modulates appetite and energy metabolism in mammals. The yak is the only large mammal that can adapt to the cold temperatures and hypoxia conditions present in the Qinghai-Tibet Plateau. However, there are no reports on ghrelin molecular characterization and expression in the hypothalamus-pituitary-digestive tract axis of the yak to date. In this study, the coding region sequence of the yak ghrelin, containing a complete ORF (351) encoding for 117 amino acids, was cloned...
September 3, 2018: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/30175852/egln1-gene-variation-in-chinese-native-cattle-and-yaks
#10
Lulan Zeng, Bizhi Huang, Fangyu Li, Ningbo Chen, Zhijie Ma, Kaixing Qu, Hong Chen, Chuzhao Lei
No abstract text is available yet for this article.
September 3, 2018: Animal Genetics
https://www.readbyqxmd.com/read/30136259/microbial-diversity-in-the-rumen-reticulum-omasum-and-abomasum-of-yak-on-a-rapid-fattening-regime-in-an-agro-pastoral-transition-zone
#11
Dan Xue, Huai Chen, Xiaolin Luo, Jiuqiang Guan, Yixin He, Xinquan Zhao
The ruminant digestive system harbors a complex gut microbiome, which is poorly understood in the case of the four stomach compartments of yak. High-throughput sequencing and quantitative PCR were used to analyse microbial communities in the rumen, reticulum, omasum, and abomasum of six domesticated yak. The diversity of prokaryotes was higher in reticulum and omasum than in rumen and abomasum. Bacteroidetes predominated in the four stomach compartments, with abundance gradually decreasing in the trend rumen > reticulum > omasum > abomasum...
August 22, 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/30134528/copy-number-variations-of-klf6-modulate-gene-transcription-and-growth-traits-in-chinese-datong-yak-bos-grunniens
#12
Habtamu Abera Goshu, Xiaoyun Wu, Min Chu, Pengjia Bao, Xuezhi Ding, Ping Yan
Copy number variation (CNV) is a significant marker of the genetic and phenotypic diversity among individuals that accounts for complex quantitative traits of phenotype and diseases via modulating gene dosage and disrupting coding regions in the genome. Biochemically, Kruppel-like factor 6 ( KLF6 ) genes plays a significant role in the regulation of cell differentiation and proliferation and muscle development. The aim of this study was to detect the distributions of KLF6 copy number variations (CNVs) in five breeds of domestic yak and to explore their effect on growth traits and gene expression...
August 21, 2018: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/30132847/regulation-by-hsp27-p53-in-testis-development-and-sperm-apoptosis-of-male-cattle-cattle-yak-and-yak
#13
Penggang Liu, Sijiu Yu, Yan Cui, Junfeng He, Qian Zhang, Juan Sun, Yufeng Huang, Xiaoqing Yang, Mixia Cao, Bo Liao, Junxing Ma
Heat shock protein 27 (Hsp27)/protein 53 (P53) plays an important role in testis development and spermatozoa regulation, but the relationship between Hsp27/P53 and infertility in cattle is unclear. Here, we focus on male cattle-yak and yak to investigate the expression and localization of Hsp27/P53 in testis tissues and to explore the influence of Hsp27/P53 on infertility. In our study, a total of 54 cattle (24 cattle-yak and 30 yak) were examined. The Hsp27 and P53 messenger RNA (mRNA) of cattle-yak were cloned, and amino acid variations in Hsp27 and P53 were found; the variations led to differences in the protein spatial structure compared with yak...
August 21, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30125429/postpartum-oestrous-cycling-resumption-of-yak-cows-following-different-calf-weaning-strategies-under-range-conditions
#14
Peipei Liu, Quanmin Dong, Shujie Liu, Allan Degen, Jiaojiao Zhang, Qiang Qiu, Xiaoping Jing, Zhanhuan Shang, Wenming Zheng, Luming Ding
Early weaning can improve body condition and reproductive performance of cows. The objectives of this study were to examine and compare oestrous cycling resumption, behaviour and blood parameters of yak cows following four different strategies of calf weaning. Twenty-six yak cows (4-8 years) and their calves (94.3 ± 2.4 days) were studied in which calves were: weaned naturally with free access to their mothers (NW; n = 13); weaned abruptly and separated permanently from their mothers (PW; n = 5); separated temporarily from their mothers for 15 days (TW; n = 5); and fitted with nose plates for 15 days, but allowed free access to their mothers (NP; n = 3)...
August 20, 2018: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/30120840/expression-and-localization-of-follicle-stimulating-hormone-receptor-in-the-yak-uterus-during-different-stages-of-the-estrous-cycle
#15
Ayimuguli Abudureyimu, Yong Cai, Shengdong Huo, Rui Ren, Rongxin Zang, Jutian Yang, Zhongren Ma, Yan Cui
Morphological changes of the uterus and alterations in its secretory activity under the influence of steroid hormones been well documented. The estrous cycle is also associated with significant changes in plasma follicle stimulating hormone (FSH), whose effects are mediated through its receptor (FSHR). Reports showed that in many mammals, FSHR were expressed in gonadal and extragonadal tissues including cervix, female reproductive tract, pituitary gland, etc. Follicle stimulating hormone (FSH) signals through endothelial FSHR directly stimulate angiogenesis and involved in the timing of birth in human, and the presence of FSHR in the placenta is essential for normal pregnance in mice...
August 18, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/30106465/distribution-of-egfr-bmp-2-and-p53-in-kidney-of-healthy-newborn-adult-and-old-highland-plateau-yaks
#16
Ting Wang, Yan Cui, Penggang Liu, Junfeng He, Qian Zhang
BACKGROUND: Kidney has long been thought to be a body's largest organ of elimination for maintain acid-base balance. In recent years, the research on kidneys has mainly focused on the structural characteristics of the kidney of single age group animals. In this Paper we used histological and immunohistochemical methods to observe and compare the structure characteristics of yak kidney and the expression of EGFR BMP-2 and p53 in the kidney of yaks of three different age groups. AIMS: To investigate histological characteristics of age-related changes in the kidney of yak and expression and localization of kidney related factors...
August 14, 2018: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/30094727/yak-and-tibetan-sheep-trampling-inhibit-reproductive-and-photosynthetic-traits-of-medicago-ruthenica-var-inschanica
#17
Hong Xiao, Zhen Peng, Chang Lin Xu, De Gang Zhang, Jin Long Chai, Tao Tao Pan, Xiao Jun Yu
Livestock grazing affects grassland stability, resilience, and productivity owing to trampling, foraging, and excretion. Over time, trampling influences a wide range of grassland components and can have lasting effects. Trampling helps maintain grassland health but may also cause its degradation. In a field experiment over two growing seasons, we simulated yak and sheep trampling at different intensities and investigated their effects on the reproductive and photosynthetic characteristics of Medicago ruthenica var...
August 9, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/30086187/warming-and-land-use-change-concurrently-erode-ecosystem-services-in-tibet
#18
Kelly A Hopping, Alan K Knapp, Tsechoe Dorji, Julia A Klein
Alpine meadows on the Tibetan Plateau comprise the largest alpine ecosystem in the world and provide critical ecosystem services, including forage production and carbon sequestration, on which people depend from local to global scales. However, the provision of these services may be threatened by climate warming combined with land use policies that are altering if and how pastoralists can continue to graze livestock, the dominant livelihood practice in this region for millennia. We synthesized findings from a climate warming and yak grazing experiment with landscape-level observations in central Tibet to gain insight into the trajectories of change that Tibet's alpine meadows will undergo in response to expected changes in climate and land use...
August 7, 2018: Global Change Biology
https://www.readbyqxmd.com/read/30081086/bacterial-communities-related-to-3-nitro-1-propionic-acid-degradation-in-the-rumen-of-grazing-ruminants-in-the-qinghai-tibetan-plateau
#19
Wei Guo, Sisi Bi, Jingpeng Kang, Ying Zhang, Ruijun Long, Xiaodan Huang, M N Shan, Robin C Anderson
The objectives of this current study were to characterize the overall rumen bacterial community in grazing yak and two sheep species (Tibetan and Small Tail Han sheep) reared in the unique environmental conditions of the Qinghai-Tibetan Plateau, as well as the bacterial community associated with the detoxification of a phytotoxin, 3-nitro-1-propionic acid (NPA), during in vitro culture with 4.2 mM NPA. Using 16S rRNA gene high-throughput sequencing, it was found that the yak rumen harbored populations showing a higher bacterial diversity compared to Tibetan sheep...
August 3, 2018: Anaerobe
https://www.readbyqxmd.com/read/30077795/survivin-expression-and-localization-in-different-organs-of-yaks-bos-grunniens
#20
Jun Liu, Yan Cui, Sijiu Yu, Yufeng Huang, Penggang Liu, Liangli Song, Juan Sun, Qian Zhang, Junfeng He
Yaks (Bos grunniens) have special physiological structures that help them adapt to high-altitude environments. Survivin is actively studied in cancer tissues, but less in normal tissues. Therefore, the aim of the present study was to analysis the relationship between survivin expression and apoptosis rate in yaks. A partial gene sequence of survivin was cloned and characterized using bioinformatics. The expression of survivin was investigated using real-time quantitative PCR (RT-qPCR) and western blot (WB) analysis and localized using immunohistochemistry (IHC)...
November 1, 2018: General and Comparative Endocrinology
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