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https://www.readbyqxmd.com/read/30465861/the-glycerin-associated-pain-gap-study
#1
Christopher A Coop, Karen Barker, Scott Dickson, Troy Baker, Kirk Waibel
No abstract text is available yet for this article.
November 19, 2018: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/30418619/sources-of-erroneous-sequences-and-artifact-chimeric-reads-in-next-generation-sequencing-of-genomic-dna-from-formalin-fixed-paraffin-embedded-samples
#2
Simon Haile, Richard D Corbett, Steve Bilobram, Morgan H Bye, Heather Kirk, Pawan Pandoh, Eva Trinh, Tina MacLeod, Helen McDonald, Miruna Bala, Diane Miller, Karen Novik, Robin J Coope, Richard A Moore, Yongjun Zhao, Andrew J Mungall, Yussanne Ma, Rob A Holt, Steven J Jones, Marco A Marra
Tissues used in pathology laboratories are typically stored in the form of formalin-fixed, paraffin-embedded (FFPE) samples. One important consideration in repurposing FFPE material for next generation sequencing (NGS) analysis is the sequencing artifacts that can arise from the significant damage to nucleic acids due to treatment with formalin, storage at room temperature and extraction. One such class of artifacts consists of chimeric reads that appear to be derived from non-contiguous portions of the genome...
November 12, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/30404015/tools-for-rapid-high-resolution-behavioral-phenotyping-of-automatically-isolated-drosophila
#3
W Ryan Williamson, Martin Y Peek, Patrick Breads, Brian Coop, Gwyneth M Card
Sparse manipulation of neuron excitability during free behavior is critical for identifying neural substrates of behavior. Genetic tools for precise neuronal manipulation exist in the fruit fly, Drosophila melanogaster, but behavioral tools are still lacking to identify potentially subtle phenotypes only detectible using high-throughput and high spatiotemporal resolution. We developed three assay components that can be used modularly to study natural and optogenetically induced behaviors. FlyGate automatically releases flies one at a time into an assay...
November 6, 2018: Cell Reports
https://www.readbyqxmd.com/read/30400159/use-of-foaming-disinfectants-and-cleaners-to-reduce-aerobic-bacteria-and-salmonella-on-poultry-transport-coops
#4
Carolee Hinojosa, David Caldwell, James Byrd, Robert Droleskey, Jason Lee, Phil Stayer, Erin Resendiz, Javier Garcia, Stephanie Klein, Denise Caldwell, Megan Pineda, Morgan Farnell
Transport coops are infrequently washed and have been demonstrated to cross-contaminate broiler carcasses. We hypothesized that peracetic acid or a chlorinated cleaner, commonly used within poultry processing plants, can also be used to disinfect transport coops when applied via a compressed air foam system (CAFS). A mixture of fresh layer manure and concentrated Salmonella Typhimurium (ST) was evenly applied to the floors of four pre-cleaned transport coops and allowed to dry for thirty minutes. Treatments consisted of a (1) water rinse only, (2) product application with a water rinse, (3) product application followed by power washing and (4) power washing followed by application of product...
November 2, 2018: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/30389971/remotely-sensed-productivity-clusters-capture-global-biodiversity-patterns
#5
Nicholas C Coops, Sean P Kearney, Douglas K Bolton, Volker C Radeloff
Ecological regionalisations delineate areas of similar environmental conditions, ecological processes, and biotic communities, and provide a basis for systematic conservation planning and management. Most regionalisations are made based on subjective criteria, and can not be readily revised, leading to outstanding questions with respect to how to optimally develop and define them. Advances in remote sensing technology, and big data analysis approaches, provide new opportunities for regionalisations, especially in terms of productivity patterns through both photosynthesis and structural surrogates...
November 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30389808/reconstructing-the-history-of-polygenic-scores-using-coalescent-trees
#6
Michael D Edge, Graham Coop
Genome-wide association studies (GWAS) have revealed that many traits are highly polygenic, in that their within-population variance is governed in part by small-effect variants at many genetic loci. Standard population-genetic methods for inferring evolutionary history are ill-suited for polygenic traits-when there are many variants of small effect, signatures of natural selection are spread across the genome and subtle at any one locus. In the last several years, several methods have emerged for detecting the action of natural selection on polygenic scores, sums of genotypes weighted by GWAS effect sizes...
November 2, 2018: Genetics
https://www.readbyqxmd.com/read/30388263/inflammation-and-vascular-permeability-correlate-with-growth-in-sporadic-vestibular-schwannoma
#7
Daniel Lewis, Federico Roncaroli, Erjon Agushi, Dominic Mosses, Ricky Williams, Ka-Loh Li, Xiaoping Zhu, Rainer Hinz, Ross Atkinson, Andrea Wadeson, Sharon Hulme, Helen Mayers, Emma Stapleton, Simon K L Lloyd, Simon R Freeman, Scott A Rutherford, Charlotte Hammerbeck-Ward, D Gareth Evans, Omar Pathmanaban, Alan Jackson, Andrew T King, David Coope
Background: Inflammation is hypothesized to be a key event in the growth of sporadic vestibular schwannoma (VS). In this study we sought to investigate the relationship between inflammation and tumor growth in vivo using the PET tracer 11C-(R)-PK11195 and dynamic contrast enhanced (DCE) MRI derived vascular biomarkers. Methods: Nineteen patients with sporadic VS (8 static, 7 growing and 4 shrinking tumors), underwent prospective imaging with dynamic 11C-(R)-PK11195 PET and a comprehensive MR protocol; including high temporal resolution DCE-MRI in fifteen patients...
November 2, 2018: Neuro-oncology
https://www.readbyqxmd.com/read/30311218/an-implementation-study-of-relationship-checkups-as-home-visitations-for-low-income-at-risk-couples
#8
Kristina Coop Gordon, James V Cordova, Patricia N E Roberson, Melanie Miller, Tatiana Gray, Katherine A Lenger, Matt Hawrilenko, Kerri Martin
Couples with the greatest need for relationship health maintenance and intervention are often least able to afford and access it; therefore, accessible, affordable, effective, and brief interventions are needed to improve relationship health for those who need it most. Consequently, this paper examined whether a brief relationship intervention could be effectively implemented with a low-income, underserved population. All enrolled participants (N = 1,312) received the Relationship Checkup, which consists of an assessment and a feedback session delivered in their homes or at a local clinic at their request...
October 11, 2018: Family Process
https://www.readbyqxmd.com/read/30291070/mobile-app-for-simplifying-life-with-diabetes-technical-description-and-usability-study-of-glucoman
#9
Kaspar S Schmocker, Fabian S Zwahlen, Kerstin Denecke
BACKGROUND: Patients with diabetes can be affected by several comorbidities that require immediate action when occurring as they may otherwise cause fatal or consequential damage. For this reason, patients must closely monitor their metabolism and inject insulin when necessary. The documentation of glucose values and other relevant measurements is often still on paper in a diabetes diary. OBJECTIVE: The goal of this work is to develop and implement a novel mobile health system for the secure collection of relevant data referring to a person's metabolis and to digitize the diabetes diary to enable continuous monitoring for both patients and treating physicians...
February 26, 2018: JMIR diabetes
https://www.readbyqxmd.com/read/30288459/tetrabutylammonium-bromide-promoted-metal-free-efficient-rapid-and-scalable-synthesis-of-n-aryl-amines
#10
Chad R Johnson, Mohd Imran Ansari, Andrew Coop
A rapid, transition metal-free, high-yielding, tetrabutylammonium bromide-promoted method of N-arylation is reported within. The optimized conditions tolerated a wide range of secondary amines and was equally effective with bromo- and chlorobenzene-including substituted aryl halides. The developed method is found to be effective for N-arylation when compared to earlier methods which involve harsh conditions, transition metals, lack of scalability, and long reaction times. Our method utilizes conventional heating only; it is readily scalable; and the products are facile to purify...
September 30, 2018: ACS Omega
https://www.readbyqxmd.com/read/30260293/do-we-prepare-patients-for-their-digital-legacy-a-survey-of-palliative-care-professionals
#11
Hazel Coop, Clare Marlow
No abstract text is available yet for this article.
September 27, 2018: Palliative Medicine
https://www.readbyqxmd.com/read/30248092/phylogeography-and-demographic-history-of-the-chagas-disease-vector-rhodnius-nasutus-hemiptera-reduviidae-in-the-brazilian-caatinga-biome
#12
Tatiana Peretolchina, Márcio G Pavan, Jessica Corrêa-Antônio, Rodrigo Gurgel-Gonçalves, Marli M Lima, Fernando A Monteiro
BACKGROUND: Rhodnius nasutus, a vector of the etiological agent Trypanosoma cruzi, is one of the epidemiologically most relevant triatomine species of the Brazilian Caatinga, where it often colonizes rural peridomestic structures such as chicken coops and occasionally invades houses. Historical colonization and determination of its genetic diversity and population structure may provide new information towards the improvement of vector control in the region. In this paper we present thoughtful analyses considering the phylogeography and demographic history of R...
September 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/30223636/preventing-human-salmonella-infections-resulting-from-live-poultry-contact-through-interventions-at-retail-stores
#13
Megin Nichols, Lauren Stevenson, Laura Whitlock, Kristy Pabilonia, Misha Robyn, Colin Basler, Tom Gomez
The number of outbreaks of Salmonella infections linked to live poultry contact increased from 1990 to 2016. In 2016, the number of human illnesses linked to live poultry was the highest reported, with more than 900 cases, including 209 hospitalizations and three deaths. Live poultry harboring Salmonella typically appear healthy but can intermittently shed bacteria in their droppings, contaminating their feathers, beaks, and the areas where they live and roam. Thus, both direct contact with poultry and indirect contact with anything in areas where animals live and roam can result in human Salmonella infection...
July 31, 2018: Journal of Agricultural Safety and Health
https://www.readbyqxmd.com/read/30138641/palmitate-treated-astrocyte-conditioned-medium-contains-increased-glutathione-and-interferes-in-hypothalamic-synaptic-network-in-vitro
#14
Ariadne de Almeida Branco Oliveira, Nayara de Freitas Martins Melo, Érica Dos Santos Vieira, Pedro Augusto Silva Nogueira, Andressa Coope, Lício Augusto Velloso, Rômulo Sperduto Dezonne, Carlos Ueira-Vieira, Francoise Vasconcelos Botelho, Juliana de Assis Silva Gomes, Renata Graciele Zanon
Excessive fat consumption increases the level of fatty acids (FAs) in the blood, which reach the hypothalamus and damage the circuit related to energy balance. In the present study, we used palmitate in a primary culture of purified astrocytes to mimic the fat-rich environment found in obesity. Our results showed increased glial fibrillary acidic protein (GFAP) reactivity in hypothalamic astrocytes compared to cortical astrocytes. In addition, palmitate-treated astrocytes showed no significant changes in cytokine expression and an upregulation of glutathione in the culture medium that may serve as an intrinsic neuroprotective property against excess FA...
November 2018: Neurochemistry International
https://www.readbyqxmd.com/read/30125763/lead-exposure-to-children-from-consumption-of-backyard-chicken-eggs
#15
Jessica H Leibler, Komal Basra, Thomas Ireland, Alyssa McDonagh, Catherine Ressijac, Wendy Heiger-Bernays, Donna Vorhees, Marieke Rosenbaum
Backyard chicken ownership is rapidly increasing in urban areas in the United States, largely as a way to provide eggs for household consumption. Despite elevated levels of environmental lead contamination in many US cities, the role of backyard chicken eggs as a pathway for lead exposure, particularly for children, has received limited scrutiny. To characterize lead exposure from consumption of backyard chicken eggs for children and predict related effects on blood lead level (BLL), we conducted a cross-sectional study of backyard chicken owners in the Greater Boston area (n = 51)...
November 2018: Environmental Research
https://www.readbyqxmd.com/read/30093601/an-update-of-wnt-signalling-in-endometrial-cancer-and-its-potential-as-a-therapeutic-target
#16
Amy Coopes, Claire E Henry, Estelle Llamosas, Caroline Elizabeth Ford
Endometrial cancer is the most common gynaecological malignancy in developed nations, and its prevalence is rising as women defer or decide not to have children and as obesity rises, both key risk factors. Despite this, treatment options remain limited, particularly for advanced or refractory disease. New genomic analyses have revealed distinct mutational profiles with therapeutic and prognostic potential. Wnt signalling, which is pivotal in embryogenesis, healing and homeostasis, is of importance in the endometrium and has been linked to carcinogenesis...
August 9, 2018: Endocrine-related Cancer
https://www.readbyqxmd.com/read/30081868/mhealth-versus-face-to-face-study-protocol-for-a-randomized-trial-to-test-a-gender-focused-intervention-for-young-african-american-women-at-risk-for-hiv-in-north-carolina
#17
Felicia A Browne, Wendee M Wechsberg, Paul N Kizakevich, William A Zule, Courtney P Bonner, Ashton N Madison, Brittni N Howard, Leslie B Turner
BACKGROUND: Disparities in the prevalence of HIV persist in the southern United States, and young African American women have a disproportionate burden of HIV as compared with young women of other racial/ethnic backgrounds. As a result, engaging young African American women in the HIV care continuum through HIV testing is imperative. This study is designed to reach this key population at risk for HIV. The study seeks to test the efficacy of two formats of a gender-focused, evidence-based, HIV-risk reduction intervention-the Young Women's CoOp (YWC)-relative to HIV counseling and testing (HCT) among young African American women between the ages of 18 and 25 who use substances and have not recently been tested for HIV...
August 6, 2018: BMC Public Health
https://www.readbyqxmd.com/read/30054213/human-parasitism-and-toxicosis-by-ornithodoros-rietcorreai-acari-argasidae-in-an-urban-area-of-northeastern-brazil
#18
Stefan Vilges de Oliveira, Karla Bitencourth, Ana Beatriz Pais Borsoi, Francisca Samya Silva de Freitas, Gerlene Castelo Branco Coelho, Marinete Amorim, Gilberto Salles Gazeta
The soft tick, Ornithodoros rietcorreai, is a parasite of the rodent, Kerodon rupestris, and, to a lesser extent, of bats living in rock cavities in Northeastern Brazil. This report describes the first recorded episodes of human parasitism by this argasid tick, reported to the Brazilian Ministry of Health in September 2017. We assessed outdoor environments, roofs, animal nests and chicken coops in five houses located in an urban area of Russas City, Ceará State, Brazil. Our results confirmed the presence of the tick in two of the assessed houses...
September 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/30045952/physical-work-demands-and-psychosocial-working-conditions-as-predictors-of-musculoskeletal-pain-a-cohort-study-comparing-self-reported-and-job-exposure-matrix-measurements
#19
Ida E H Madsen, Nidhi Gupta, Esben Budtz-Jørgensen, Jens Peter Bonde, Elisabeth Framke, Esben Meulengracht Flachs, Sesilje Bondo Petersen, Annemette Coop Svane-Petersen, Andreas Holtermann, Reiner Rugulies
OBJECTIVES: Determining exposure to occupational factors by workers' job titles is extensively used in epidemiological research. However, the correspondence of findings regarding associations to health between job exposure matrices (JEMs) and individual-level exposure data is largely unknown. We set out to examine the prospective associations of physical work demands and psychosocial working conditions with musculoskeletal pain, comparing JEMs with individual-level self-reported exposures...
October 2018: Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/30032145/frailty-and-associated-factors-among-centenarians-in-the-5-coop-countries
#20
Marie Herr, Bernard Jeune, Stefan Fors, Karen Andersen-Ranberg, Joël Ankri, Yasu Arai, Sarah Cubaynes, Brigitte Santos-Eggimann, Dina Zekry, Marti Parker, Yasuhiko Saito, François Herrmann, Jean-Marie Robine
BACKGROUND: The global number of centenarians is still strongly growing and information about the health and healthcare needs of this segment of the population is needed. This study aimed to estimate the prevalence of frailty among centenarians included in a multinational study and to investigate associated factors. METHODS: The 5-COOP study is a cross-sectional survey including 1,253 centenarians in 5 countries (Japan, France, Switzerland, Denmark, and Sweden)...
2018: Gerontology
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