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Chun-Yu Lin, Yu-Han Chung, Ya-Fang Shi, Bor-Show Tzang, Tsai-Ching Hsu
Both human parvovirus B19 (B19V) and human bocavirus (HBoV) are known to be important human pathogens of the Parvoviridae family. Our earlier investigation demonstrated that both B19V-VP1u and HBoV-VP1u have a significantly disruptive effect on tight junctions (TJs) in A549 cells, implying the essential role of parvovirus in airway infection and lung injury. However, no direct evidence that B19V-VP1u and HBoV-VP1u induce lung injury exists. The present study further investigates the induction of lung injury by B19V-VP1u and HBoV-VP1u in naïve Balb/c mice following subcutaneous injection of PBS, recombinant B19V-VP1u or HBoV-VP1u...
2018: PloS One
Xinhua Wu, Zhangrong Chen, Ying Yang, Yu Dong, Hong Liu, Shiquan Kuang, Kailiang Luo
The aim of the present study was to investigate the impact of carbobenzoxy-Leu-Leu-leucinal (MG-132) on myocardial remodeling in rats with myocardial infarction (MI) and investigate the possible underlying mechanisms. The rat model of MI was established, followed by administration of MG-132 (MG group), pyrrolidine dithiocarbamic acid (PDTC group) or normal saline (MI group) for 28 days. The expression of nuclear factor-κB (NF-κB) p65, interleukin 1β (IL-1β) and matrix metalloproteinase 2 (MMP-2), as well as the total volume of collagen and the ratio of type I/III collagen were then detected...
August 2018: Experimental and Therapeutic Medicine
Katriana A Popichak, Sean L Hammond, Julie A Moreno, Maryam F Afzali, Donald S Backos, Richard D Slayden, Steven Safe, Ronald B Tjalkens
Inflammatory activation of glial cells promotes loss of dopaminergic neurons in Parkinson's disease (PD). The transcription factor, Nuclear Factor-kappa B (NF-κB), regulates the expression of multiple neuroinflammatory cytokines and chemokines in activated glial cells that are damaging to neurons. Thus, inhibition of NF-κB signaling in glial cells could be a promising therapeutic strategy for the prevention of neuroinflammatory injury. Nuclear orphan receptors in the NR4A family, including NR4A1 (Nur77) and NR4A2 (Nurr1), can inhibit the inflammatory effects of NFκB but there are no approved drugs that target these receptors...
August 15, 2018: Molecular Pharmacology
Zong-Chao Liu, Yan Jiang, Chen-Yi Huang, Yong Liu, Zhang-Chao Wei, Shi-Gui Liu, Zhi-Jiang Fu, Chuan Ma
This paper aimed to investigate the role of Duhuo Jisheng decotion (DHJSD) in delaying human disc degeneration and its possible molecular mechanism. The intervertebral disc specimens were divided into normal and degenerated groups according to Pfirrmann classfication. The expressions of TNF-α, IL-1β, MMP-3 and MMP-13 in intervertebral disc tissue were detected by Western blot and PCR. Then degenerated human primary NPCs were cultured in vitro, the viability of NPCs treated with stromal cell-derived factor-1 (SDF-1,10 μg·L⁻¹)and various concentrations of DHJSD was assessed by the CCK-8 assay, and the appropriate concentration was screened...
July 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Lijuan Chai, Kun Zhou, Shaoxia Wang, Han Zhang, Na Fan, Jie Li, Xiaofeng Tan, Limin Hu, Xiang Fan
BACKGROUND/AIMS: Psoralen and bakuchiol are the main active compounds found in the traditional Chinese medicine Psoralea corylifolia L., and have been used to treat osteoporosis. This study aims to investigate the anti-osteoporosis effects of these two compounds using osteoclasts precursor differentiation and bone absorption assays in vitro. METHODS: Primary mouse osteoclasts precursor cells were induced by M-CSF (macrophage colony stimulating factor) plus RANKL (receptor activator of nuclear factor kappa-B ligand) in vitro...
August 15, 2018: Cellular Physiology and Biochemistry
Chenglong Liu, Fei Zhang, Haihua Liu, Fang Wei
PURPOSE: To evaluate the role of CX3CL1 and NF-κB in the lumbar disc herniation induced neuropathic pain. METHODS: After LDH induced by implantation of autologous nucleus pulposus (NP) on the left L5 nerve root was established, mechanical thresholds and thermal hyperalgesia were tested at relevant time points during an observation period of 28 days. Expression of CX3CL1 and NF-κBin the dorsal root ganglion (DRG) were performed by using Western blotting and RT-PCR...
July 2018: Acta Cirúrgica Brasileira
Zhi-Zhun Mo, Zhi-Xiu Lin, Zi-Ren Su, Lin Zheng, Hui-Lin Li, Jian-Hui Xie, Yan-Fang Xian, Tie-Gang Yi, Shui-Qing Huang, Jian-Ping Chen
Angelica sinensis (AS, Danggui in Chinese) is an important herbal component of various traditional formulae for the management of asthenia and its tonic effects. Although AS has been shown to ameliorate cognitive damage and nerve toxicity in D-galactose (D-gal)-elicited senescent mice brain, its effects on liver and kidney injury have not yet been explored. In this work, mice were subjected to hypodermic injection with D-gal (200 mg/kg) and orally gavaged with AS (20, 40, or 80 mg/kg) once a day for 8 successive weeks...
August 15, 2018: Journal of Medicinal Food
Ken Shirato, Tomoko Koda, Jun Takanari, Junetsu Ogasawara, Takuya Sakurai, Hideki Ohno, Takako Kizaki
We recently reported that ETAS 50, a standardized extract from the Asparagus officinalis stem, exerted anti-inflammatory effects on ultraviolet-B- (UV-B-) irradiated normal human dermal fibroblasts (NHDFs) by inhibiting nuclear factor- κ B p65 nuclear import and the resulting interleukin-1 β (IL-1 β ) expression. To further elucidate the antiphotoaging potency of ETAS 50, we examined the anti-inflammatory effects on UV-B-irradiated NHDFs by focusing on the stress-activated mitogen-activated protein kinase (MAPK) and Akt signaling pathways...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Jie Liu, Yiming Hao, Ziyuan Wang, Fang Ni, Yu Wang, Lingxiao Gong, Baoguo Sun, Jing Wang
The characteristic constituent and anti-inflammatory activity of 5-n-alkylresorcinols (ARs) from 21 wheat bran samples in China were investigated in this study. The amount of ARs ranged from 697 μg/g to 1732 μg/g in the tested samples, which were composed of five different homologues. Among these homologues, C19:0 and C21:0 were the most abundant, followed by C17:0, C23:0 and C25:0. Moreover, the mRNA expression of IL-1β, IL-6 and TNF-α in LPS-activated RAW264.7 macrophage cells were significantly inhibited by ARs supplementation...
August 14, 2018: Journal of Agricultural and Food Chemistry
Xiaofang Sui, Shuqian Yu, Lin Dou, Xiehui Chen, Xuejie Li, Jun Yang, Yanan Su, Shuyue Wang, Fengling Wang, Jian Li
Endothelial dysfunction is an early marker of atherosclerosis. Previous studies have indicated that microRNA (miR)‑291b‑3p regulates the metabolism of lipids and glucose in the liver via targeting adenosine monophosphate‑activated kinase α1 and transcription factor p65. The present study investigated whether miR‑291b‑3p mediated H2O2‑mediated endothelial dysfunction. The level of apoptosis of EOMA mouse endothelial cells was analyzed by terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labelling staining...
August 10, 2018: International Journal of Molecular Medicine
Ruohua Zhang, Hongmin Li, Qin Guo, Lulu Zhang, Jie Zhu, Jin Ji
Propionibacterium acnes induces inflammatory and plays a vital role in the formation of comedones through activation of inflammatory cells, keratinocytes, and sebocytes. Sirtuin6 (SIRT6), along with ADP-ribosyltransferase and deacetylase, has been proposed to mediate various biological functions, including inflammation. Nevertheless, no strong experimental evidence has been provided to support the effect of SIRT6 in treatment of inflammatory situation. Therefore, this study addressed the inhibitory effect of SIRT6 against P...
August 13, 2018: Journal of Cellular Biochemistry
Jun Jiang, Yitong Xu, Hongjiu Ren, Muli Wudu, Qiongzi Wang, Xin Song, Hongbo Su, Xizi Jiang, Lihong Jiang, Xueshan Qiu
BACKGROUND: Makorin RING zinc finger-2 (MKRN2) belongs to the makorin RING zinc finger family and is a novel ubiquitin E3 ligase targeting the p65 subunit of NF-κB to negatively regulate inflammatory responses; however, the relationship between MKRN2 and tumorigenesis remains unclear. In this study, we clarified the role of MKRN2 in non-small cell lung cancer (NSCLC). METHODS: Tumor specimens collected from 261 NSCLC patients from 2013 to 2017 were retrieved from the Pathology Archive of the First Affiliated Hospital of China Medical University, and we performed assays to evaluate MKRN2 expression and to determine the impact of MKRN2 silencing and overexpression on NSCLC-cell migration and invasion...
August 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
M K Seo, E J Park, S Y Ko, E W Choi, S Kim
Kefir is a fermented product from yeast and lactic acid bacteria, and has been associated with various health benefits including relieving inflammatory bowel disease. Recently, it has been shown that gram-positive bacteria produce extracellular vesicles (EV). The EV could be appearing as potentially important mediators of cell to cell interaction. In this study, we explored the role of kefir grain Lactobacillus-derived EV in modulating inflammation responses via alleviating the production of inflammatory cytokines in tumor necrosis factor-α (TNF-α)-induced inflammation in Caco-2 cells and the 2,4,6-trinitrobenzene sulfonic acid-induced inflammatory bowel disease mouse model...
August 9, 2018: Journal of Dairy Science
Bin Wang, Yufan Zhang, Wei Jiang, Li Zhu, Kaili Li, Kai Zhou, Dongsheng Dai, Shan Chang, Min Fang
Protein glycosylation, attaching glycans covalently onto amino acid side chains of protein by various glycosyltransferase, is the most common post-translational modification. The UDP-GalNAc transferase 3 (GANLT3), encoded by Galnt3, transfers N-acetyl-d-galactosamine to hydroxyl groups of the side chains of Ser/Thr residues, initiating mucin type O-glycosylation of proteins. Most researches as yet focus on the involvement and abnormal expression of GALNT3 in various tumors. In this study, we found that GALNT3 was significantly decreased in the lungs after influenza A virus (IAV) infection in mice...
August 9, 2018: Biochemical and Biophysical Research Communications
Yan Zhang, Xiaoli Su, Fangfang Zou, Tengjuan Xu, Pinhua Pan, Chengping Hu
BACKGROUND: Obstructive sleep apnea (OSA)-associated chronic kidney disease is mainly caused by chronic intermittent hypoxia (CIH) triggered renal damage. This study aims to investigate the role of toll-like receptor-4 (TLR4) in underlying mechanism involved chronic intermittent hypoxia (CIH)-induced renal damage. METHODS: C57BL/6J mice with normal TLR4 (TLR4 WT) or deficient TLR4 (TLR4 KO) were divided into four groups and exposed to normal air (NA) and CIH: TLR4 WT + NA, TLR4 KO + NA, TLR4 WT + CIH, and TLR4 KO + CIH...
August 11, 2018: Sleep & Breathing, Schlaf & Atmung
Siyi Su, Xiaoyu Li, Siting Li, Pengfei Ming, Yingying Huang, Yanli Dong, Hongyan Ding, Shibin Feng, Jinchun Li, Xichun Wang, Yu Li, Jinjie Wu
Rutin, found widely in traditional Chinese medicine materials, is used to treat eye swelling and pain, hypertension, and hyperlipidemia. In the present study, a mouse mastitis model induced by lipopolysaccharide (LPS) was established to explore rutin's inhibitory mechanism on mastitis via nuclear factor kappa B (NF-κB) inflammatory signaling and the relationship between NF-κB signaling and endoplasmic reticulum (ER) stress. Mice were divided into six groups: Control group, LPS model group, LPS + rutin (25, 50, and 100 mg/kg) and LPS + dexamethasone (DEX) group...
August 11, 2018: Inflammopharmacology
Xiu-Li Gu
AIM: To investigate the regulation of microRNA-124 -(miRNA-124) on NF-κB pathway from H2O2-induced apoptosis and oxidative stress in human lens epithelial cells (hLEC). METHODS: The MTT (3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was used to detect hLEC -viability. HLECs were divided into Blank, H2O2, mimics (miRNA-124 mimics) + H2O2, NC+ H2O2, pyrrolidine dithiocarbamate (PDTC; NF-κB signaling pathway inhibitor) + H2O2, and inhibitors (miRNA-124 inhibitors) + PDTC + H2O2 groups...
August 10, 2018: Pharmacology
Ji Yeon Choi, In Jun Yeo, Ki Cheon Kim, Won Rack Choi, Jae-Kyung Jung, Sang-Bae Han, Jin Tae Hong
BACKGROUND: Alzheimer's disease, which is pathologically characterized by an excessive accumulation of amyloid beta (Aβ) fibrils, is a degenerative brain disease and the most common cause of dementia. In a previous study, it was reported that an increased level of CHI3L1 in plasma was found in AD patients. We investigated the inhibitory effect of 2-({3-[2-(1-cyclohexen-1-yl)ethyl]-6,7-dimethoxy-4-oxo-3,4-dihydro-2-quinazolinyl}sulfanyl)-N-(4-ethylphenyl)butanamide (K284-6111), an inhibitor of chitinase 3 like 1 (CHI3L1), on memory impairment in Aβ1-42 -infused mice, and microglial BV-2 cells and astrocytes...
August 11, 2018: Journal of Neuroinflammation
Kathiroli Sujithra, Subramani Srinivasan, Dhananjayan Indumathi, Veerasamy Vinothkumar
Therapeutic approaches based on dietary compounds obtained from food products to handle diabetes involving oxidative stress and inflammation. Garlic is a common spice and has a long history as a folk remedy. Allyl methyl sulfide (AMS) is a potential garlic-derived organosulfur compound displaying a substantial range of optimistic actions in various diseases. Herein, we investigated the potential role of AMS in ameliorating the effects of oxidative stress and inflammation in the liver of streptozotocin (STZ)-induced experimental rats...
August 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Wei Wang, Jiye Wang
BACKGROUND Toll-like receptor 4 (TLR4)-mediated signaling has been implicated in invasion, metastasis, and survival of various cancers. Activation of TLR4 can promote cyclooxygenase-2 (COX-2) and nuclear factor-κB (NF-κB). However, little is known about the effects of TLR4/COX-2 in prostate cancer (PCa). MATERIAL AND METHODS In our study, TLR4 and COX-2 expressions were detected by quantitative real-time reverse transcription PCR (qRT-PCR) in PCa tissues (n=34). Cell proliferation was measured by Cell Counting Kit-8 (CCK-8) and carboxyfluorescein succinimidyl ester (CFSE) assays...
August 11, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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