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Xu Wang, Shuai Lu, Yankai Xiao, Ling Xu, Lingling Zhou, Junyan Hu, Bo Li, Chengwu Zeng, Yangqiu Li
Background: T cell immunodeficiency is a common feature in patients with different kinds of hematological disease such as T cell non-Hodgkin lymphoma (T-NHL), B cells NHL (B-NHL), NK/T cell NHL (NK/T-CL) and acute myeloid leukemia (AML). In our recent research, we found that significantly lower expression levels in MALT1 and NF-κB were related to suppression of T cell activation. Therefore, this study was conducted to further investigate the role of downregulating MALT1 in the development of immunodeficiency in T cells...
2018: ImmunoTargets and Therapy
Eun-Yi Ko, Soo-Jin Heo, Su-Hyeon Cho, WonWoo Lee, Seo-Young Kim, Hye-Won Yang, Ginnae Ahn, Seon-Heui Cha, Seung-Hae Kwon, Myeong Seon Jeong, Kang Pa Lee, You-Jin Jeon, Kil-Nam Kim
The anti-inflammatory effects of 3‑bromo‑5‑(ethoxymethyl)‑1,2‑benzenediol (BEMB) from Polysiphonia morrowii were evaluated in lipopolysaccharide (LPS)-induced RAW264.7 cells and zebrafish embryo. BEMB showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO) and reactive oxygen species (ROS), and the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in the LPS-activated RAW264.7 cells and zebrafish embryo without cytotoxicity. Moreover, BEMB suppressed the protein and mRNA expression levels of nuclear factor (NF)-κB (p65 and inhibitor of NF-κB [IκB]-A) in RAW264...
December 8, 2018: International Immunopharmacology
Hongmei Li, Guochun Ou, Yonglong He, Long Ren, Xiaohong Yang, Mei Zeng
TAK1 is closely associated with the NF-κB and MAPK signaling pathways. In the present study, we aimed to explore the relationship between TAK1 and gout as well as the effects of resveratrol on TAK1 activity and MSU crystal-induced inflammation. The expression levels of total TAK1 and phosphorylated TAK1 in gout patients were detected by western blotting. The influence of resveratrol on TAK1 activity and MSU crystal-induced inflammation was investigated in THP-1 cell and murine models of gout. The results showed that TAK1 and p-TAK1 were highly expressed in gouty arthritis patients...
December 7, 2018: International Immunopharmacology
Jie Yu, Yi Zheng, Xue Ping Han, Hao Peng, Li Juan Pang, Feng Li, Yunzhao Chen, Xiaobin Cui
Esophageal squamous cell carcinoma (ESCC) is a highly invasive disease with a poor long-term survival rate. Although there has been progress in understanding the pathogenesis of ESCC, there are currently no molecular biomarkers that are used in routine clinical practices to determine prognosis. Therefore, the aim of this study was to determine a small immunohistochemical (IHC) panel that could predict the prognosis of patients with ESCC. Phospholipase C epsilon-1 (PLCE1), IKKα, IKBα, p65, and p53 were highly expressed in ESCC tissues...
December 8, 2018: Human Pathology
Paulraj Kanmani, Hojun Kim
Intestinal epithelial cells (IECs) respond to intruders and their cellular molecules by displaying inflammatory state that can be abrogated by probiotics. However, the molecular mechanisms underlying the beneficial activity of probiotic strains have yet to be elucidated. This study was conducted to investigate whether probiotic strains have immunoregulatory effects in IECs, and how they respond to bacterial lipopolysaccharide (LPS) in vitro. Caco2 cells were stimulated with LABs and followed by LPS. The expression of different cytokines that involved in toll-like receptor (TLR) signaling was analyzed...
December 11, 2018: BioFactors
Weifeng Huang, Yongmei Cao, Yujing Liu, Feng Ping, Jiawei Shang, Zhongwei Zhang, Yingchuan Li
The balance between Ang II/AT1R and Ang-(1-7)/Mas plays a pivotal role in the development of lipopolysaccharides (LPS)-induced acute respiratory distress syndrome. However, the mechanisms underlying the balancing process still remain unclear. Here we investigated the roles of nuclear factor (NF)-κB and p53 in regulating AT1R and Mas expression. The results demonstrated that Ang II pretreatment resulted in downregulation of Mas and upregulation of AT1R, phosphorylated p65, and apoptosis in LPS-treated Human pulmonary microvascular endothelial cells (HPMVECs), but had no effect on p53 expression...
December 7, 2018: Journal of Cellular Physiology
Lijian Cheng, Zhenhong Chen, Lihua Wang, Yunping Lan, Lihua Zheng, Fangpu Wu
Peripheral inflammation in male C57BL/6 mice was induced by intraplantar injection of 20 μL complete freund's adjuvant (CFA) in the left hind paw. Mice were randomly divided into three groups: Sham, CFA, and propofol+CFA. Mechanical allodynia was assessed by von Frey analysis, and heat hyperalgesia was detected by exposure of the plantar surface to a beam of radiant heat. Propofol significantly attenuated the severity and duration of CFA-induced pain hypersensitivity, heat hyperalgesia, and paw edema. Propofol inhibited CFA-induced microglia activation, and markedly decreased CFA-induced ionized calcium binding adapter molecule 1 (IBA-1) expression...
December 9, 2018: Journal of Cellular Biochemistry
Xiumei Chen, Jianzhong Lin, Tingting Hu, Zhiyun Ren, Linnan Li, Irbaz Hameed, Xiaoyu Zhang, Chen Men, Yan Guo, Di Xu, Yiyang Zhan
Oxidized low-density lipoprotein (Ox-LDL)-induced endothelial cell injury plays a crucial role in the pathogenesis of atherosclerosis (AS). Plasma galectin-3 (Gal-3) is elevated inside and drives diverse systemic inflammatory disorders, including cardiovascular diseases. However, the exact role of Gal-3 in ox-LDL-mediated endothelial injury remains unclear. This study explores the effects of Gal-3 on ox-LDL-induced endothelial dysfunction and the underlying molecular mechanisms. In this study, Gal-3, integrin β1, and GTP-RhoA in the blood and plaques of AS patients were examined by ELISA and western blot respectively...
December 10, 2018: Journal of Cellular Physiology
Zhongbiao Wang, Dennis W Ashley, Lingwei Kong, Jing Kang, Don K Nakayama, Paul S Dale
BACKGROUND: Implantation of a retrievable vena cava filter (VCF) is an effective method for preventing pulmonary embolism. Retrieval of filters, however, may be difficult due to intimal hyperplasia and inflammation in the cava wall. The transcription factor nuclear factor-kappaB (NF-κB) plays an important role in regulation of numerous genes participating in the inflammatory and proliferative responses of cells. The present study was to determine whether VCF implantation resulted in activation of NF-κB in the venous neointima...
December 10, 2018: Cardiovascular and Interventional Radiology
Di Wang, Peng Liu, Yue Zhang, Hui-Ying Liu, Di Shen, Yi-Qun Che
Activated B-cell-like diffuse large B-cell lymphoma (ABC-DLBCL) is a common subtype of non-Hodgkin's lymphoma and is very likely to infiltrate the bone marrow. Over 30% of patients are converted to relapsed/refractory DLBCL after first-line rituximab combined with cyclophosphamide, doxorubicin, vincristine, and prednisone therapy, with a poor prognosis. Our aim was to identify molecular markers that might be utilized to predict relapsed/refractory ABC-DLBCL patients. Hence, we collected bone marrow aspirate smears from 202 patients with ABC-DLBCL and detected expression of bone marrow molecular marker proteins by immunocytochemistry...
2018: BioMed Research International
Xi Shi, Shiwei Qiu, Wei Zhuang, Caiji Wang, Shili Zhang, Na Yuan, Fukang Yuan, Yuehua Qiao
Background: Follicle-stimulating hormone (FSH) has multiple biological functions. It is currently considered that FSH can inhibit cervical cancer, and our aim was to explore the underlying molecular mechanisms. Materials and methods: An in vivo experiment using nude mice injected with HeLa cells was performed. Flow cytometry, western blotting, and real-time quantitative PCR analyses were done. Results: Twenty one days after injection of HeLa cells, the subcutaneous tumor mass was significantly lower ( P <0...
2018: OncoTargets and Therapy
Hong-Yun He, Lu Ren, Tao Guo, Yi-Hao Deng
Ischemic stroke often induces excessive neuronal autophagy, resulting in brain damage; meanwhile, inflammatory responses stimulated by ischemia exacerbate neural injury. However, interactions between neuronal autophagy and microglial inflammation following ischemic stroke are poorly understood. CX3CL1/fractalkine, a membrane-bound chemokine expressed on neurons, can suppress microglial inflammation by binding to its receptor CX3CR1 on microglia. In the present study, to investigate whether autophagy could alter CX3CL1 expression on neurons and consequently change microglial inflammatory activity, middle cerebral artery occlusion (MCAO) was established in Sprague-Dawley rats to model ischemic stroke, and tissues from the ischemic penumbra were obtained to evaluate autophagy level and microglial inflammatory activity...
February 2019: Neural Regeneration Research
Ayman Hyder
Nutrients may modulate immunity through their transcription factors that act on both metabolic and immunity genes. It has been shown that the transcription factor of lipid ligands PPARγ physically binds the gene promoter of the peptidoglycan recognition protein (PGlyRP3), which showed anti-inflammatory action in vitro. It is hypothesized in the present work that olive oil feeding protects against toxicity of DSS-induced colitis via activation of the lipid transcription factor PPARγ that stimulates the anti-inflammatory PGlyRP3...
December 7, 2018: International Immunopharmacology
Min Su, Chao Guo, Meizhen Liu, Xiaoliu Liang, Bin Yang
In our previous studies, vitamin C (VC) exerts potent pharmacological activities against liver injury (LI). Therefore, this report was designed to use network pharmacology-based strategy to predict therapeutic targets of VC against LI, and further to investigate the pharmacological molecular mechanisms. Pathological targets of LI were identified, followed by acquisition of verified targets of VC. After constructing target-functional protein interaction network of VC against LI, the core therapeutic targets of VC against LI were obtained...
December 5, 2018: International Immunopharmacology
Manabu Kakinohana, Eizo Marutani, Kentaro Tokuda, Kotaro Kida, Shizuko Kosugi, Shingo Kasamatsu, Aurora Magliocca, Kohei Ikeda, Shinichi Kai, Masahiro Sakaguchi, Shuichi Hirai, Ming Xian, Masao Kaneki, Fumito Ichinose
Delayed paraplegia complicates the recovery from spinal cord ischemia or traumatic spinal cord injury. While delayed motor neuron apoptosis is implicated in the pathogenesis, no effective treatment or preventive measures is available for delayed paraplegia. Hydrogen sulfide exerts anti-apoptotic effects. Here, we examined effects of hydrogen sulfide breathing on the recovery from transient spinal cord ischemia. Breathing hydrogen sulfide starting 23h after reperfusion for 5h prevented delayed paraplegia after 5min of spinal cord ischemia...
December 7, 2018: Free Radical Biology & Medicine
Yanru Fan, Liping Ou, Jiaxin Fan, Luo Li, Xiaohou Wu, Chunli Luo, Yingying Gao, Lingfang Niu
OBJECTIVE: To investigate how hepatocyte cell adhesion molecule (hepaCAM) regulates cancer energy metabolism through hypoxia-inducible factor (HIF-1α) in renal cell carcinoma (RCC). MATERIALS AND METHODS: The expression of hepaCAM and HIF-1α in RCC tissue samples was examined by immunohistochemistry. Glucose consumption and lactate production assays were used to detect metabolic activity in RCC cell lines. P65 and IκB kinase (IKKβ) mRNA and protein expression was detected using quantitative real-time polymerase chain reaction and western blotting, respectively...
December 4, 2018: Urology
Yan-Min Yang, Rong-Hua Shi, Chuan-Xiang Xu, Lei Li
The objective of this study was to investigate BRD4 expression in patients with essential hypertension (EH) and its effects on the blood pressure in spontaneously hypertensive (SHR) rats. BRD4 expression was detected by quantitative real-time polymerase chain reaction and western blot in 163 patients with EH and its relation to systolic blood pressure and diastolic blood pressure were analyzed accordingly. In vivo, rats were divided into WKY (Wistar-Kyoto rats), SHR (spontaneously hypertensive rats), SHR + JQ1 (BRD4 inhibitor), and SHR + Vehicle control...
November 22, 2018: Journal of the American Society of Hypertension: JASH
Xin Jin, Donglin Ding, Yuqian Yan, Hui Li, Bo Wang, Linlin Ma, Zhenqing Ye, Tao Ma, Qiang Wu, Daniel N Rodrigues, Manish Kohli, Rafael Jimenez, Liguo Wang, David W Goodrich, Johann de Bono, Haidong Dong, Heshui Wu, Runzhi Zhu, Haojie Huang
Aberrant expression of programmed death ligand-1 (PD-L1) in tumor cells promotes cancer progression by suppressing cancer immunity. The retinoblastoma protein RB is a tumor suppressor known to regulate the cell cycle, DNA damage response, and differentiation. Here, we demonstrate that RB interacts with nuclear factor κB (NF-κB) protein p65 and that their interaction is primarily dependent on CDK4/6-mediated serine-249/threonine-252 (S249/T252) phosphorylation of RB. RNA-seq analysis shows a subset of NF-κB pathway genes including PD-L1 are selectively upregulated by RB knockdown or CDK4/6 inhibitor...
December 3, 2018: Molecular Cell
Se-Ho Park, Seung-Su Lee, Myun-Ho Bang, Sung Kwan Jo, Kwang-Hwan Jhee, Seun-Ah Yang
This study was undertaken to determine the effects of enzyme-treated Zizania latifolia (ETZL) and of its major compound tricin on skin photo-aging and to investigate the mechanisms involved. It was found ETZL and tricin suppressed matrix metalloproteinase (MMP) production and increased type I-procollagen production in UVB-irradiated human dermal fibroblasts (HDFs). Furthermore, ETZL and tricin significantly up-regulated the expressions of the antioxidant enzymes HO-1 and SOD1, reduced UVB-induced reactive oxygen species (ROS) generation and mitogen-activated protein kinase (MAPK) induction by ROS and thereby attenuated activator protein-1 (AP-1) expression...
December 9, 2018: Bioscience, Biotechnology, and Biochemistry
Tabea Riedlinger, Robert Liefke, Johanna Meier-Soelch, Liane Jurida, Andrea Nist, Thorsten Stiewe, Michael Kracht, M Lienhard Schmitz
Increasing evidence shows that many transcription factors execute important biologic functions independent from their DNA-binding capacity. The NF-κB p65 (RELA) subunit is a central regulator of innate immunity. Here, we investigated the relative functional contribution of p65 DNA-binding and dimerization in p65-deficient human and murine cells reconstituted with single amino acid mutants preventing either DNA-binding (p65 E/I) or dimerization (p65 FL/DD). DNA-binding of p65 was required for RelB-dependent stabilization of the NF-κB p100 protein...
December 7, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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