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Francisca Taciana Sousa Rodrigues, Marcos Romário Matos de Souza, Camila Nayane de Carvalho Lima, Francisco Eliclécio Rodrigues da Silva, Deiziane Viana da Silva Costa, Cláudio Costa Dos Santos, Fábio Miyajima, Francisca Cléa F de Sousa, Silvânia Maria Mendes Vasconcelos, Tatiana Barichello, João Quevedo, Michael Maes, David F de Lucena, Danielle Macedo
Major depressed patients show increased bacterial translocation with elevated plasma levels of lipopolysaccharide (LPS), which may trigger immune-inflammatory and neuro-oxidative responses. Recently, an animal model based on chronic LPS administration was developed which was associated with long-lasting depressive-like and neuro-oxidative alterations in female mice. The aim of the current study was to investigate behavioral, neuroimmune and neuroprogressive alterations in female mice 6 weeks after LPS chronic exposure...
October 5, 2018: Journal of Psychiatric Research
Mélanie Pagès, Kristian W Pajtler, Stéphanie Puget, David Castel, Nathalie Boddaert, Arnault Tauziède-Espariat, Stéphanie Picot, Debily Marie-Anne, Marcel Kool, David Capper, Christian Sainte-Rose, Fabrice Chrétien, Stefan M Pfister, Torsten Pietsch, Jacques Grill, Pascale Varlet, Felipe Andreiuolo
Ependymoma with RELA fusion has been defined as a novel entity of the revised WHO 2016 classification of tumours of the central nervous system (CNS), characterised by fusion transcripts of the RELA gene and consequent pathological activation of the NFkB pathway. These tumours represent the majority of supratentorial ependymomas in children. The validation of diagnostic tools to identify this clinically relevant ependymoma entity is essential. Here, we have used interphase fluorescent in situ hybridisation (FISH) for C11orf95 and RELA, immunohistochemistry (IHC) for p65-RelA and the recently developed DNA-methylation based classification besides conventional histopathology and compared the precision of the methods in 40 supratentorial paediatric brain tumours diagnosed as ependymomas in the past years...
October 16, 2018: Brain Pathology
Yang Liu, Yuanhang Liu, Xuecheng Huang, Jingchang Zhang, Lihui Yang
OBJECTIVE: Coronary microembolization (CME) is a common complication during the percutaneous coronary intervention (PCI). CME-induced local myocardial inflammation and myocardial apoptosis are the primary causes of progressive cardiac dysfunction. Curcumin exerts a protective role in various cardiovascular diseases; however, its effects in CME are yet to be clarified. Therefore, the current study investigated the effects of curcumin on myocardial inflammatory responses, myocardial apoptosis, and cardiac dysfunctions induced by CME in rats...
October 15, 2018: Journal of Cellular Biochemistry
Vivek B Mathema, Wanna Chaijaroenkul, Kesara Na-Bangchang
OBJECTIVES: To evaluate the cytotoxic activity of atractylodin and its potential effects on heme oxygenase (HO)-1 production, STAT1/3 phosporylation and major NF-κB protein expression in the cholangiocarcinoma-associated cell line CL-6. METHODS: Standard MTT assay was used for accessing antiproliferative activity on CL-6 cells. Normal human embryonic fibroblast (OUMS) cell was taken as control cell line. Colony formation and wound healing assay were conducted to access the effects of atractylodin on cell proliferation and directional migration activity of CL-6 cells...
October 15, 2018: Journal of Pharmacy and Pharmacology
Jing Shen, Xin Sun, Bo Pan, Shoubo Cao, Jingyan Cao, Dehai Che, Fang Liu, Shuai Zhang, Yan Yu
Background: Tumor-associated macrophage (TAM) is emerging as one of the important complications in cancer promotion. Interleukin-17 (IL-17), a potent pro-inflammatory cytokine, plays an active role in promoting M2 macrophage differentiation (TAMs are M2-like phenotypes). In this study, we aimed to evaluate that IL-17 stimulates key phenotypic and functional signatures of M2 macrophages associated with cancer progression in non-small-cell lung cancer (NSCLC) patients. Patients and methods: The markers and cytokines of M2 macrophages were detected in THP-1-derived macrophages and mouse peritoneal macrophages treated with IL-17...
2018: Cancer Management and Research
Hiroki Yoshioka, Tsunemasa Nonogaki, Shiori Fukaya, Yoshimi Ichimaru, Akito Nagatsu, Masae Yoshikawa, Hirohisa Fujii, Makoto Nakao
BACKGROUND: The current study aimed to investigate the hepatoprotective effects of Sasa veitchii extract (SE) on carbon tetrachloride (CCl4 )-induced liver fibrosis in mice. METHODS: Male C57BL/6J mice were intraperitoneally injected with CCl4 dissolved in olive oil (1 g/kg) twice per week for 8 weeks. SE (0.1 mL) was administered orally once per day throughout the study, and body weight was measured weekly. Seventy-two hours after the final CCl4 injection, mice were euthanized and plasma samples were collected...
October 15, 2018: Environmental Health and Preventive Medicine
Ming Zhong, Cheng Zhong, Pei Hu, Wen Cui, Guanghui Wang, Huijei Gao, Chao Liu, Zhiqiang Liu, Zhihua Li, Chunxia Li, Eiichi Gohda
Dendritic cells (DCs) play key regulatory roles in tumor immunity: increased activity of DCs infiltrating tumor tissues leads to enhancement of tumor immunity. Functions of DCs are also modulated by tumor cell-derived factors. Here, we investigated the effects of low molecular weight oyster polysaccharide (LMW-OPS) on differentiation and function of bone marrow-derived DCs (BMDCs) exposed to a conditioned medium (CM) obtained from spheres of stemness-high colorectal cancer cell lines CMT93 and CT26. The CM containing a detectable level of TGF-β1 was found to down-regulate the surface expression of major histocompatibility complex class II of BMDCs and to inhibit the potency of BMDCs to stimulate T cells...
October 12, 2018: International Immunopharmacology
Li-Chun Zhang, Yu Wang, Wei Liu, Xue-Min Zhang, Miao Fan, Min Zhao
AIMS: To study the protective effects and mechanisms of human umbilical cord mesenchymal stem cells (hUCMSCs) and overexpression of antioxidant gene SOD2 on lung injury by establishing a rat model of paraquat (PQ)-induced lung injury. MAIN METHODS: The hUCMSCs cell line overexpressed SOD2 was established. After intraperitoneal injection of PQ solution (24 mg/kg) 3 h later, the different groups of hUCMSCs cell lines were injected through the tail veins of rats...
October 12, 2018: Life Sciences
Zhaoting Yang, Chengye Zhang, Wenbo Qi, Yanhua Xuan, Yan Cui
The role of Hedgehog (HH)/ glioma-associated oncogene homolog 1 (GLI1) pathway has been implicated in a variety of cancer entities, and the targeted pathway inhibition mediated by GLI1 is of therapeutic relevance. However, its oncogenicity and cross-talks with other cancer pathways including PI3K/Akt/NFκB, which modulates the HH/GLI1 signal strength, have rarely been explored in colorectal adenocarcinoma. We assessed the expression of GLI1 and its relationship with other cancer stemness genes, cell cycle markers, epithelial-mesenchymal transition (EMT), PI3K/Akt/NFκB signaling pathway genes, and HIF1α in 100 paraffin-embedded colorectal adenocarcinoma tissue samples using immunohistochemistry...
October 12, 2018: Experimental Cell Research
Milena Armacki, Anna Katharina Trugenberger, Ann K Ellwanger, Tim Eiseler, Christiane Schwerdt, Lucas Bettac, Dominik Langgartner, Ninel Azoitei, Rebecca Halbgebauer, Rüdiger Groß, Tabea Barth, André Lechel, Benjamin M Walter, Johann M Kraus, Christoph Wiegreffe, Johannes Grimm, Annika Scheffold, Marlon R Schneider, Kenneth Peuker, Sebastian Zeißig, Stefan Britsch, Stefan Rose-John, Sabine Vettorazzi, Eckhart Wolf, Andrea Tannapfel, Konrad Steinestel, Stefan O Reber, Paul Walther, Hans A Kestler, Peter Radermacher, Thomas Fe Barth, Markus Huber-Lang, Alexander Kleger, Thomas Seufferlein
Dysregulated intestinal epithelial apoptosis initiates gut injury, alters the intestinal barrier, and can facilitate bacterial translocation leading to a systemic inflammatory response syndrome (SIRS) and/or multi-organ dysfunction syndrome (MODS). A variety of gastrointestinal disorders, including inflammatory bowel disease, have been linked to intestinal apoptosis. Similarly, intestinal hyperpermeability and gut failure occur in critically ill patients, putting the gut at the center of SIRS pathology. Regulation of apoptosis and immune-modulatory functions have been ascribed to Thirty-eight-negative kinase 1 (TNK1), whose activity is regulated merely by expression...
October 15, 2018: Journal of Clinical Investigation
Yan Zhao, Sangyan Chen, Fang Shen, Dan Long, Ting Yu, Mengru Wu, Xuechi Lin
Our previous studies have identified that silencing oncoprotein 18 (Op18)/stathmin via RNA interference (RNAi) inhibited autocrine interleukin‑10 (IL‑10) and enhanced the sensitivity to Taxol in NCI‑H1299 non‑small cell lung cancer cells. In this study, whether autocrine IL‑10 regulates Op18/stathmin signaling in NCI‑H1299 cells was examined by neutralizing IL‑10 using a targeted antibody. In vitro neutralization of IL‑10 by anti‑IL‑10 antibody impaired the capacities of the NCI‑H1299 cells to proliferate, form colonies and migrate...
October 15, 2018: Oncology Reports
Xin Chen, Shujun Zhou, Hui Li
Viral and bacterial severe pneumonia are leading causes of mortality across the globe. Evodiamine (Evo), a botanical alkaloid, has anti‑inflammatory and antibacterial properties. In the present study, the effect of Evo on severe pneumonia induced by methicillin‑susceptible Staphylococcus aureus (MSSA) following cytomegalovirus (CMV) reactivation, and its mechanism, were evaluated. In vitro, the protein and mRNA expression levels of inflammatory cytokines were determined by enzyme‑linked immunosorbent assay and reverse transcription‑quantitative polymerase chain reaction analysis, respectively...
October 11, 2018: International Journal of Molecular Medicine
Yang Gao, Hui Liu, Hongzhi Wang, Hailong Hu, Hongjuan He, Ning Gu, Xiao Han, Qian Guo, Dong Liu, Shuang Cui, Hongjiang Shao, Chengjun Jin, Qiong Wu
The aim of the present study was to investigate the effect and therapeutic potential of baicalin in breast cancer. Baicalin is used to treat inflammatory diseases. The effects of baicalin were assessed in breast cancer MCF-7 and MDA-MB‑231 cells, and human breast cancer xenograft mice. Cells were treated with 0, 20 or 30 µM baicalin for 48 h, while xenograft mice were treated with intraperitoneal injection of 0, 100 or 200 mg/kg baicalin for 30 days. The results demonstrated that treatment with baicalin dose-dependently suppressed breast cancer cell invasion, migration and proliferation, and also induced G1/S-phase cell cycle arrest in vitro and in vivo...
October 12, 2018: International Journal of Oncology
Lei Ding, Huan Gu, Zhenwei Lan, Qingchun Lei, Wenxue Wang, Jiangfei Ruan, Min Yu, Jie Lin, Qinghua Cui
The prognosis of patients with colorectal cancer (CRC) remains poor owing to diagnosis typically occurring at advanced stages of the disease. The understanding of the molecular regulatory signatures of CRC may lead to the identification of biomarkers for the early detection, prevention and clinical intervention of CRC. Epidemiological studies have indicated that cyclooxygenase‑1 (COX‑1) serves an active function in colon carcinogenesis. However, the molecular mechanism underlying COX‑1 regulation in CRC remains unknown...
October 12, 2018: Oncology Reports
Sarah McKenna, Taylor Burey, Jeryl Sandoval, Leanna Nguyen, Odalis Castro, Suma Gudipati, Jazmin Gonzalez, Karim C El Kasmi, Clyde J Wright
Sepsis is a major cause of neonatal morbidity and mortality. The current paradigm suggests that neonatal susceptibility to infection is explained by an innate immune response that is functionally immature. Recent studies in adults have questioned a therapeutic role for IFNβ in sepsis; however, the role of IFNβ in mediating neonatal sensitivity to sepsis is unknown. We evaluated the transcriptional regulation and expression of IFNβ in early neonatal (P0) and adult murine models of endotoxemia (IP LPS, 5 mg/kg)...
2018: Frontiers in Immunology
Qiong Wang, Xin Zhou, Yongjian Zhao, Jun Xiao, Yao Lu, Qi Shi, Yongjun Wang, Hongyan Wang, Qianqian Liang
Background: Rheumatoid arthritis (RA) is a chronic autoimmune disorder, characterized by an increased number of M1-like macrophages in the joints. Polyphyllin I (PPI), one of the main components in the Rhizoma of Paris polyphyllin, displays a selective inhibitory effect on various tumor cells. Here we sought to investigate the anti-rheumatoid arthritis effects and mechanisms of PPI on macrophages in vivo and in vitro . Materials and Methods: In vitro , primary bone marrow-derived macrophages (BMMs) and peritoneal elucidated macrophages (PEMs) were stimulated by lipopolysaccharide (LPS) and Interferon (IFN)-γ and then treated with PPI...
2018: Frontiers in Immunology
Cheng Zheng, Jia-Feng Lin, Zhong-Hao Lin, Wei-Qian Lin, Saroj Thapa, Yuan-Zheng Lin, Hao Lian, Zhi-Rui Liu, Jia-Hui Chen, Xiao-Wei Li
With the chronic ischemia persisting after acute myocardial infarction, the accompanying low-degree inflammation and subsequent fibrosis result in progression of cardiac remodeling and heart failure. Recently, Sodium Houttuyfonate (SH), a pure compound extracted from Houttuynia cordata , has been confirmed exerting anti-inflammatory and anti-fibrotic effects under diseased situations. Here, we aimed to investigate whether SH could reverse the cardiac remodeling post-myocardial infarction by alleviating cardiac inflammation and fibrosis...
2018: Frontiers in Pharmacology
Lu Fan, Ye Fan, Li Liu, Weiwei Tao, Xin Shan, Yu Dong, Lin Li, Sen Zhang, Hanqing Wang
Chelerythrine (CH), is a kind of benzo[c] phenanthridine alkaloid isolated from plants such as Chelidonium, with pharmacological activities as antitumor, antibiosis and anti-inflammation. However, few studies have demonstrated whether CH could protect against lipopolysaccharide (LPS)-induced acute lung injury (ALI), and the underlying mechanism is also uncertain. The purpose of the present study was to investigate the anti-inflammatory effects of CH on LPS-induced ALI in mice and in RAW264.7 cells. In this study, we demonstrated that treatment with CH significantly ameliorated LPS-induced pathological changes in the lung...
2018: Frontiers in Pharmacology
Yao-Xing Dou, Jiang-Tao Zhou, Tong-Tong Wang, Yan-Feng Huang, Vicky Ping Chen, You-Liang Xie, Zhi-Xiu Lin, Jian-Sheng Gao, Zi-Ren Su, Hui-Fang Zeng
Background: Bruceine D (BD) is a major bioactive component isolated from the traditional Chinese medicinal plant Brucea javanica which has been widely utilized to treat dysentery (also known as ulcerative colitis [UC]). Methods: To improve the water solubility and absolute bioavailability of BD, we developed a self-nanoemulsifying drug delivery system (SNEDDS) composing of MCT (oil), Solutol HS-15 (surfactant), propylene glycol (co-surfactant) and BD. The physicochemical properties and pharmacokinetics of BD-SNEDDS were characterized, and its anti-UC activity and potential mechanism were evaluated in TNBS-induced UC rat model...
2018: International Journal of Nanomedicine
B F Zhang, M L Cheng, Y P Wang, Q Zhang, L Yu, X K Zhao, M Mu, Y Liu
Objective: To observe the effects of blueberry and nuclear expression of transcription factor-кb (NF-кb) p65 in an experimental rat model of liver fibrosis. Methods: Forty-five Sprague-Dawley rats were randomly divided into isotonic saline control group (A); model group (B); blueberry juice prevention group (C, 15 g/kg); dan-shao-hua-xian capsule prevention group (D, 1 g/kg); and blueberry juice + dan-shao-hua-xian capsule prevention group (E). Rat liver fibrosis model was established by covalent compound carbon tetrachloride (CCl(4))...
August 20, 2018: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
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